The invention belongs to the technical field of analysis and detection, and discloses a Boc-D-phenylglycinol isomer detection method which comprises the following steps: preparing a
raw material Boc-D-phenylglycinol into a
test solution, diluting the
test solution to obtain a control solution, and preparing a Boc-L-phenylglycinol control substance and a Boc-D-phenylglycinol control substance into a
system applicability solution; respectively injecting the
system applicability solution, the
test solution and the contrast solution into a high performance liquid chromatograph for detection, and calculating the content of Boc-L-phenylglycinol in the
raw material Boc-D-phenylglycinol according to a main component self-contrast method. The detection method disclosed by the invention is strong in specificity, good in
linearity, good in
repeatability and good in stability of a test solution, can be used for detecting two isomers, namely Boc-D-phenylglycinol and Boc-L-phenylglycinol, and can be used for controlling the quality of an initial
raw material and an intermediate of the oxaggolil
sodium, so that the quality of the oxaggolil
sodium is improved.