1714 results about "Test solution" patented technology

The invention discloses a quality detection method for carpesium abrotanoides formula granules, and belongs to the technical field of traditional Chinese medicine detection. The detection method comprises the following steps: testing the total content of the specific chromatogram, neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid, and testing the content of carabrone. The method for testing the specific chromatogram and the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid comprises the following steps: a, preparing a neochlorogenic acid reference substance solution, a chlorogenic acid reference substance solution and a cryptochlorogenic acid reference substance solution; b, preparing a test solution; and c, carrying out liquid chromatography detection, and calculating the total content of neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid. The carabrone content test comprises the following steps: A, preparing a carabrone reference substance solution; b, preparing a test solution; and C, carrying out liquid chromatography detection, and calculating the carabrone content. The quality of the carpesium abrotanoides formula granules is effectively controlled, and the medication effectiveness and safety of the carpesium abrotanoides formula granules are guaranteed.

The invention discloses a method for detecting the hydrazine content in a medicine containing carbidopa. The invention provides a method for detecting hydrazine content in a medicine containing carbidopa, which comprises the following steps: (1) mixing a sample to be detected and an aldehyde substance in a solvent under the action of acid, deriving hydrazine in the sample to be detected and the aldehyde substance to obtain a test solution, and (2) detecting the content of hydrazine in the test solution. Compared with the prior art, the method has the advantages that the carbidopa does not need to be removed through pretreatment, hydrazine introduced in the determination process can be ignored, operation is simple, derivatization time and sample injection time do not need to be accurately controlled, repeatability is good, and quantification is accurate.

The invention belongs to the field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of tolperidone hydrochloride, methyl hesperidin and vitamin B6 in Naoluotong capsules, the method comprises three steps of preparation of a mixed reference solution, preparation of a test solution and content determination, and the chromatographic conditions of the content determination are as follows: a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column and a C18 chromatographic column; a mobile phase is methanol (A)-0.1% glacial acetic acid solution (B), gradient elution is carried out for 0-5 min, and 96% of B is obtained; 5-6 min, 96%-60% of B is selected; 6-20 min, the content of B is 60%-50%; the volume flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the detection wavelength is 291 nm; the sample size is 1 microliter. According to the method, synchronous detection of three components (tolperidone hydrochloride, methyl hesperidin and vitamin B6) with remarkable physicochemical property difference can be realized, the chromatographic condition is simple, and the detection speed is high.

The invention relates to a detection method for determining isotope abundance of a 15N or 18O labeled nitrate sample by using an organic mass spectrum. The detection method comprises the following steps: (1) dissolving the 15N or 18O labeled nitrate sample in a methanol aqueous solution; (2) taking the supernatant, filtering with a filter membrane, and diluting with a methanol aqueous solution to obtain a solution to be detected; and (3) detecting the solution to be detected by using a high-resolution liquid chromatograph-mass spectrometer, selecting a mass spectrum in a peak appearance time window, recording relative mass spectrum signal intensities with mass-to-charge ratios of 61.988 and 62.985, and calculating to obtain the 15N isotope abundance. Or recording the relative mass spectrum signal intensities with the mass-to-charge ratios of 61.988, 63.993, 65.997 and 68.001, and calculating to obtain the abundance of the < 18 > O isotope. Compared with the prior art, the method has the advantages that the sample pretreatment time is greatly shortened, the economic cost is low, and the test result is accurate.

The invention provides a construction method of fingerprint spectrums of katsumadai medicinal material, standard decoction and formula granules thereof, which comprises the following steps: preparing a mixed reference solution from alpinetin, pinocembrin, cardamonin and alnudinone; preparing a test solution from the alpinia katsumadai medicinal material, the standard decoction or the formula granules; respectively detecting the reference solution and the test solution by adopting a high performance liquid chromatography, and constructing fingerprints of the alpinia katsumadai medicinal material, the standard decoction and the formula granules of the alpinia katsumadai medicinal material; wherein the conditions of the high performance liquid chromatography are as follows: a mixed solution of acetonitrile and methanol is taken as a mobile phase A, a phosphoric acid aqueous solution with the volume concentration of 0.08%-0.12% is taken as a mobile phase B, and a gradient elution procedure is adopted. The method has the advantages of being simple, stable and high in specificity, and a comprehensive, effective and rapid evaluation method is provided for quality control of alpinia katsumadai medicinal materials, standard decoction and formula granules of the alpinia katsumadai medicinal materials.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The present disclosure provides a method for determining content of olaflur in toothpaste by liquid chromatography-tandem mass spectrometry, comprising the following steps: obtaining to-be-tested toothpaste; dissolving the to-be-tested toothpaste in water and then adding methanol for dilution to obtain a to-be-tested solution; and placing the to-be-tested solution in a liquid chromatograph for determination under following chromatographic conditions: a C4 column; a mobile phase as a combination of phase A being methanol with 0.1% formic acid and phase B being acetonitrile, or a combination of phase A being methanol with 0.1% formic acid and phase B being water; and isocratic or gradient elution. The method can analyze the purity of organic olaflur in fluoride-containing anti-caries toothpaste and olaflur raw materials, and enables quantitative analysis of olaflur by an internal or internal standard method. Furthermore, the method can accurately and qualitatively identify olaflur through characteristic ions.

The invention provides a method for measuring silver content in boron-containing water, which comprises the following steps: preparing a blank standard solution and a standard working solution by using a first-stage diluent and a second-stage diluent, and respectively pretreating the blank standard solution, the standard working solution and a to-be-measured sample solution by using a third-stage diluent; the first-stage diluent comprises a nitric acid solution, the second-stage diluent comprises a boron solution, and the third-stage diluent comprises a nitric acid solution; and measuring the silver content of the to-be-measured sample solution by adopting inductively coupled plasma emission spectrometry. According to the determination method provided by the invention, the silver content in the boron-containing water of the nuclear power plant can be accurately and rapidly detected through pretreatment of the test solution, the problem of boron interference in the boron-containing water is solved, the detection accuracy of the silver content is improved, and support is provided for chemical control of nuclear power loop water.

The invention discloses a construction method of a gout compound decoction fingerprint as well as the fingerprint and application thereof, and belongs to the technical field of analysis and detection. The construction method of the fingerprint spectrum comprises the following steps: taking a test solution prepared from the gout compound decoction; the test solution is subjected to HPLC-SPD detection, so that the fingerprint spectrum of the gout compound decoction is obtained, and the HPLC detection conditions are that a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, and a C18 chromatographic column is used for detecting the fingerprint spectrum of the gout compound decoction. An aqueous solution containing 0.1%-0.2% of phosphoric acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the flow velocity is 0.8-1 mL / min, the column temperature is 25 DEG C, the sample injection volume is 5-10 [mu] L, and a diode array detector is 190-400 nm full-wave band scanning. The chemical components in the gout compound decoction are comprehensively and systematically analyzed, and a basis is provided for quality control and in-depth study of a pharmacodynamic material basis.

The invention relates to the technical field of medicine analysis and detection, and particularly discloses a method for detecting the content of protein in a 3D-MLA adjuvant, which comprises the following steps: (1) preparing a test solution: firstly dissolving the 3D-MLA adjuvant by using a hydrochloric acid solution, performing 300-600W ultrasonic treatment for 2-5min, centrifuging 10000-13000g for 4-7min, and taking supernate; adding a compound enzyme into the supernate for enzymolysis; then adding concentrated hydrochloric acid, uniformly mixing, hydrolyzing for 20 + / -2 hours in a nitrogen atmosphere at 105-115 DEG C, blow-drying, and dissolving with a hydrochloric acid solution to obtain a test sample stock solution; adding a derivatization reagent into the test sample stock solution, and carrying out derivatization treatment, extraction and dilution to obtain a test sample solution; and (2) high performance liquid chromatography determination. According to the detection method provided by the invention, the residual quantity of the host cell protein in the 3D-MLA can be rapidly and accurately determined, and the quality control of the 3D-MLA is realized.

The invention discloses a method for detecting a chiral isomer of 6-hydroxy-8-chloro ethyl caprylate. The method comprises the following steps: carrying out gas chromatography detection on a test solution containing R-6-hydroxy-8-chloro ethyl caprylate and S-6-hydroxy-8-chloro ethyl caprylate, wherein the gas chromatography detection is carried out on the test solution containing R-6-hydroxy-8-chloro ethyl caprylate and S-6-hydroxy-8-chloro ethyl caprylate; the conditions of the gas chromatography detection are as follows: a chromatographic column is selected from a chiral chromatographic column containing beta-cyclodextrin; the chromatographic column adopts programmed heating in the detection process, the initial column temperature is 120-180 DEG C, the final column temperature is 190-220 DEG C, and the heating rate is 0.5-2.0 DEG C / min. The method is based on a gas chromatographic method, adopts a beta-cyclodextrin-containing chiral chromatographic column, and is combined with programmed heating at a specific initial column temperature heating rate, so that the two chiral isomers of 6-hydroxy-8-chloro ethyl caprylate can be separated and detected, the sensitivity is high, the specificity is good, and the separation degree meets the standard; the method has important significance on preparation of single-configuration lipoic acid.

The invention discloses a thermal detection system and a chiral substance identification method, and relates to the technical field of micro-nano optics, the thermal detection system and the chiral substance identification method, based on the structure of the thermal detection system and the steps of the chiral substance identification method, a chiral substance to be detected is configured into a corresponding solution to be detected; a solution to be detected is sampled and placed in the absorber, the surface plasmon effect generated when the metal resonance layer receives chiral light irradiation is utilized, a corresponding thermal signal is output, then the thermal signal is captured and analyzed through the thermal detection system, and high-precision identification of chiral substances is achieved. Compared with a traditional circular dichroism and circular polarization fluorescence spectrophotometer device, the technical scheme provided by the invention is simple in structure, convenient to operate and relatively low in requirements on the concentration and the volume of a detected sample.

The invention relates to a content determination method of a traditional Chinese medicine composition fingered citron drink. The traditional Chinese medicine composition is prepared from the following medicinal raw materials: fingered citron, orange peel, malt, liquorice, Chinese date, purple perilla, citron, almond and donkey-hide gelatin. The content determination method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution and the test solution by high performance liquid chromatography; a mobile phase adopted by the high performance liquid chromatography is an acetonitrile and formic acid aqueous solution, and the elution mode is gradient elution. According to the content determination method, the content of various components can be simply, conveniently and rapidly determined, and richer chemical information and more comprehensive basis are provided for quality evaluation of the components.

The invention relates to a method for detecting bacterial endotoxin in a melphalan hydrochloride bulk drug, and belongs to the technical field of drug analysis. The invention provides a test solution for detecting bacterial endotoxin in a melphalan hydrochloride raw material medicine, which comprises a test product melphalan hydrochloride raw material medicine, a 2-8 DEG C sulfobutyl beta-cyclodextrin sodium water solution and calcium and / or magnesium ions. The detection method disclosed by the invention is simple to operate, the sample can be dissolved in BET water without reanalysis, and the detection result is accurate; no organic solvent is used for preparing a test solution, so that the safety to operators is good, and bacterial endotoxin interference experiments prove that no interference effect exists; and the detection limit of the bacterial endotoxin in the melphalan hydrochloride bulk drug can reach 0.2 EU / mg.

The invention provides a heparin precursor content detection method and application thereof, and the detection method comprises the following steps: (S1) preparing a heparin precursor solid sample to be detected into a heparin precursor test solution, or diluting a heparin precursor liquid sample to be detected into the heparin precursor test solution; carrying out enzymolysis on the heparin precursor test solution or the heparin precursor reference substance solution by adopting heparinase III to obtain heparin precursor test solution enzymatic hydrolysate or heparin precursor reference substance enzymatic hydrolysate; determining the peak area of the enzymatic hydrolysate by adopting a high performance liquid chromatography method; (S2) performing linear regression by applying a least square method, and establishing a mathematical relationship between the peak area Y of the heparin precursor reference substance enzymatic hydrolysate and the concentration X of the heparin precursor reference substance solution, namely Y = kX + b; and calculating the concentration of the heparin precursor in the liquid sample or the content of the heparin precursor in the solid sample according to the established mathematical relationship. The liquid phase detection method provided by the invention is higher in accuracy and good in precision, and can provide a detection result more intuitively.

The invention relates to a fingerprint establishment method, a detection method and a content determination method of a traditional Chinese medicine composition mild drink. The traditional Chinese medicine composition comprises the following raw materials: lily, pawpaw, Chinese yam, poria cocos, cinnamon, orange peel, rhizoma polygonati, radix polygonati officinalis and mulberry. The fingerprint establishment method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution and the test solution by high performance liquid chromatography; a mobile phase adopted by the high performance liquid chromatography is an acetonitrile and formic acid aqueous solution, and the elution mode is gradient elution. According to the fingerprint spectrum of the traditional Chinese medicine composition and the content determination method, the traditional Chinese medicine composition can be effectively represented, the content of various components can be simply, conveniently and rapidly determined, and richer chemical information and more comprehensive basis are provided for quality evaluation of the traditional Chinese medicine composition.

The present invention provides a method for detecting the content of effective components in a polyamide epichlorohydrin wet strength agent, and belongs to the technical field of analysis and detection. The detection method comprises the following steps: (1) preparing a PAE test solution; (2) obtaining a reaction solution 1 through an acid hydrolysis reaction; (3) obtaining a reaction solution 2 through an esterification reaction; (4) using a gas chromatograph-mass spectrometer to test the response value of diethyl adipate in the sample to be tested; (5) drawing a standard curve to calculate the concentration of diethyl adipate in the sample to be tested; (6) excluding the concentrations of diethyl adipate and adipic acid in the test sample; (7) calculating the content of effective components in the polyamide epichlorohydrin wet strength agent sample to be tested by a formula. The method is used for detecting the content of effective components in a polyamide epichlorohydrin wet strength agent, can accurately calculate the content of PAE effective components in the wet strength agent, significantly improves the accuracy and reliability of the determination, reduces the detection cost and operation difficulty, and has wide applicability.

The invention relates to a fingerprint establishment method, a detection method and a content determination method of a traditional Chinese medicine composition fingered citron beverage. The traditional Chinese medicine composition is prepared from the following medicinal raw materials: fingered citron, orange peel, malt, liquorice, Chinese date, purple perilla, citron, almond and donkey-hide gelatin. The fingerprint establishment method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution and the test solution by high performance liquid chromatography; a mobile phase adopted by the high performance liquid chromatography is an acetonitrile and formic acid aqueous solution, and the elution mode is gradient elution. According to the fingerprint spectrum of the traditional Chinese medicine composition and the content determination method, the traditional Chinese medicine composition can be effectively represented, the content of various components can be simply, conveniently and rapidly determined, and richer chemical information and more comprehensive basis are provided for quality evaluation of the traditional Chinese medicine composition.

The invention discloses a rapid qualitative and quantitative detection method for quantitative analysis of multiple components by single marker of rhodiola rosea, astragalus membranaceus and safflower capsules, and belongs to a detection and analysis technology. According to the method, three traditional Chinese medicines, namely rhodiola rosea, astragalus membranaceus and safflower carthamus, are identified on a thin-layer plate by adopting a thin-layer chromatography method and a same test solution, the pretreatment of a sample and a reference medicinal material only needs 3 hours, 265 mL of a solvent is adopted, spots are clear, and the method is rapid; according to the established single-detection double-evaluation method for salidroside and calycosin-7-glucoside, only an ultraviolet detector is needed, detection is carried out under the wavelength of 270 nm, chromatographic peaks do not interfere with one another, single-detection double-evaluation is achieved, the efficiency is high, and the cost is low. The invention breaks through a traditional identification mode that N traditional Chinese medicine varieties are identified, N test solutions and N thin-layer plates are prepared, N times of development are needed, and N medicinal materials are identified, and establishes a rapid thin-layer identification method for three traditional Chinese medicines on one thin-layer plate by adopting the same test solution and each reference medicinal material supernatant subjected to methanol ultrasonic treatment.

The invention discloses a breast lump removing capsule / granule fingerprint construction method and a fingerprint thereof, and belongs to the field of traditional Chinese medicine quality standard research and application. Respectively injecting the test solution and the single reference solution into a high performance liquid chromatograph, carrying out gradient elution, and carrying out chromatographic analysis to obtain various chromatograms; performing mass spectrometry on the test solution to obtain a mass spectrometry result; taking chromatographic peaks existing in the chromatogram of the test solution as common peaks, and identifying chemical components of the chromatographic peaks in the breast lump removing capsule / particle fingerprint spectrum by combining the chromatogram of the single reference substance solution and the mass spectrum result of the test solution, so as to obtain the breast lump removing capsule / particle fingerprint spectrum. The detection method for the breast lump removing capsule / particle fingerprint spectrum has the advantages of being simple, convenient, stable, high in precision and good in reproducibility, the quality of the breast lump removing capsule / particle can be evaluated more comprehensively and scientifically, and the quality and the curative effect of a product are guaranteed.

This invention provides a testing device and method for corrosion and wear testing of sliding guide rails under corrosive environments. The device includes a support module, a drive module, a guide rail, a test slider, a tension sensor, a pull rod, and a test tank module. The test slider is slidably mounted on the guide rail and contains a counterweight to simulate a load. The drive module drives the pull rod to slide along the length of the guide rail. The tension sensor is connected between the pull rod and the test slider. The test tank module holds the test solution to simulate a corrosive environment. This invention features a compact overall structure, simple component installation, and a simple slider force transmission method. It can realistically simulate the corrosion and wear phenomena of the sliding guide rail moving pair under seawater conditions. Furthermore, the adjustable counterweight and replaceable test solution enable this invention to conduct corrosion and wear tests on moving pairs under different load conditions and corrosive environments.

The invention provides a traditional Chinese medicine formula granule spectrum similarity evaluation system, and relates to the technical field of traditional Chinese medicine, and the system comprises: a spectrum acquisition module, which is used for separating active components with similar polarity in a compound salvia miltiorrhiza formula granule test solution by gradient elution based on chromatographic conditions to obtain a characteristic spectrum; and the analysis processing module is used for carrying out analysis processing on overlapped chromatographic peaks in the characteristic spectrum, distinguishing characteristic signals corresponding to main active components and trace components, and realizing separation and identification of characteristic peaks of different components in the mixture so as to obtain a characteristic peak analysis result. According to the method, the standard characteristic chromatogram database is constructed through gradient elution and deconvolution processing, and the accuracy and standardization of quality evaluation of the traditional Chinese medicine formula granules are improved.

The invention discloses a headspace gas chromatography detection method for simultaneously determining multiple residual solvents in pharmaceutical adjuvant hydroxypropyl methylcellulose. The headspace gas chromatography detection method comprises the following steps: preparing a reference solution containing the residual solvents; preparing a test solution of hydroxypropyl methylcellulose; respectively injecting the reference solution and the test solution into a headspace gas chromatograph for testing, and calculating the content of each residual solvent by peak area according to an external standard method; through accurate control of gas chromatography conditions, the method can accurately, rapidly and simultaneously detect the contents of residual methanol, isopropanol, dichloromethane, trichloromethane, benzene, trichloroethylene, dioxane and toluene in the pharmaceutical adjuvant hydroxypropyl methylcellulose, and has the advantages of simplicity and convenience in operation, high accuracy and sensitivity, good reproducibility and the like.

The invention provides a construction method and application of a fingerprint spectrum of a liver-warming decoction, and belongs to the technical field of medicine detection.The construction method includes the steps that a test solution is prepared through the liver-warming decoction, acetonitrile (A)-0.08-0.12 wt% formic acid aqueous solution (B) serves as a mobile phase, and the fingerprint spectrum of the liver-warming decoction is obtained through high performance liquid chromatography detection; and a standard fingerprint spectrum is established and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the liver-warming decoction. According to the construction method of the fingerprint spectrum of the liver-warming decoction, main medicinal material components in the liver-warming decoction can be detected through high performance liquid chromatography, so that the quality condition of the liver-warming decoction can be reflected more comprehensively.

The invention discloses a KLHL11 antibody high-precision detection method based on an enzyme-linked immunosorbent assay. The detection method comprises the following steps: preparing a microwell plate pre-coated with an anti-human KLHL11 antibody, dividing micropores into a plurality of detection groups, and adding KLHL11 protein or peptide fragment solutions with different concentrations, a to-be-detected solution with unknown concentration and a contrast solution into each detection group. The detection precision of a current detection instrument, the operation capability level of an operator and the reliability of the current detection instrument and the operation capability level are evaluated by executing enzyme-linked immunosorbent assay operation steps including sample filling, incubation, washing, color development and optical density measurement, obtaining the optical density value of a detection group and calculating the mean value, the standard deviation and the variation index of each group. By establishing a standard curve of optical density and concentration, the concentration of an unknown sample can be calculated, and the performance of an instrument in different concentration ranges can be verified. In addition, the problems of drifting and cross contamination of the instrument are further detected by setting a detection group with a relatively large time interval and a high-low concentration gradient experiment.

The invention discloses a method for determining the content of phosphorus in activated carbon for wine, and relates to the field of detection of food processing assistants.The method comprises the steps that phosphorus standard solutions and phosphorus determination reagents are mixed to prepare phosphorus standard solutions with different concentrations, and phosphorus standard series working solutions are prepared; extracting phosphorus in the activated carbon by using aqua regia, preparing a to-be-detected solution by using the same method as the standard solution, and respectively determining absorbance of the phosphorus standard series working solution and the to-be-detected solution by using a spectrophotometer; and quantifying by adopting an external standard method, and calculating the phosphorus content in the activated carbon sample for filtering the wine. The method provided by the invention can realize accurate qualitative and quantitative analysis of the phosphorus content in the activated carbon for filtered liquor, effectively solves the problem that no standard method for detecting the phosphorus content in the activated carbon for filtered liquor exists in the liquor industry on the premise of meeting the analysis accuracy, and is simple and convenient, low in detection cost, good in repeatability of the analysis method and high in stability.

The invention discloses a separation-free and signal-amplification-free quantitative analysis method for extracellular vesicles, which comprises the following steps: assembling a sulfydryl-modified aptamer on the surface of a gold nanoparticle through an Au-S bond to form a gold nanoparticle-aptamer compound; the aptamer is designed according to a specific membrane protein receptor on an extracellular vesicle; incubating the gold nanoparticle-aptamer compound and an extracellular vesicle to-be-detected solution, and after incubation, specifically binding the gold nanoparticle-aptamer compound with EVs to form a large-particle compound; under laser irradiation, recording the Tyndall effect of the to-be-detected liquid by using a camera, and reading the Tyndall effect intensity value by using image analysis software.

The invention provides a test device and method for evaluating chlorine resistance of a material. The test device comprises a water treatment unit; a chlorine dioxide generating unit; the storage tank is used for mixing the purified water and the chlorine dioxide therein to form a test solution; the test system comprises a water tank and a test unit, the test unit comprises at least one sample rack and a test device, the sample rack is used for fixing a test sample, and the test device is used for monitoring the performance of the test sample; the circulating system comprises a circulating pipeline and a circulating pump, the circulating pipeline is used for communicating the storage tank with the water tank to form a circulating path, and the circulating pump is used for enabling the test solution to circularly flow in the circulating path; and the wastewater treatment unit is communicated with the circulating pipeline and is used for reducing the test solution separated from the circulating pipeline and discharging the test solution out of the device. The test device is provided with the circulating system, so that various parameters of the test solution can be kept stable, and meanwhile, the operability, the safety and the environmental protection are obviously improved.

The invention relates to the technical field of medicine analysis and detection, in particular to a method for qualitatively analyzing small molecule compounds in Xinnaoning capsules by using a UPLC-MS (Ultra Performance Liquid Chromatography-Mass Spectrometry) technology. The small molecule compounds comprise polar small molecule compounds and weak polar small molecule compounds. The method comprises the following steps: preparing a test solution; carrying out UPLC / Q-TOF MS detection; and performing qualitative analysis. Through qualitative analysis of polar and weak polar small molecule compounds in the Xinnaoning capsule, a material basic component library is established, and a theoretical basis and technical support are provided for more comprehensive and effective control of product quality.

The invention discloses a method for detecting an intermediate acetylglucose of tetraacetyl by an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method. The method comprises the following steps: (1) preparing a reference substance solution, namely dissolving acetylglucose by adding a diluent, and diluting to prepare the reference substance solution; (2) preparation of a test solution: taking tetraacetyl, adding a diluent solvent, and diluting to prepare the test solution; and (3) detecting the content of acetyl glucose in the test sample by adopting an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and taking octadecylsilane chemically bonded silica as a filler in an adopted chromatographic column. According to the method disclosed by the invention, the intermediate acetyl glucose of the tetraacetyl substance is detected based on an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and CAD parameters, a chromatographic column, a mobile phase, a diluent and a sample concentration are selected, so that the aims of effectively separating and accurately detecting the acetyl glucose in the sample can be fulfilled; the method has the advantages of simplicity, sensitivity, high accuracy, high repeatability, high specificity, good durability, high precision and the like, and has certain guiding significance on carbohydrate compound detection.