1290 results about "Test solution" patented technology

The invention relates to a detection method for determining isotope abundance of a 15N or 18O labeled nitrate sample by using an organic mass spectrum. The detection method comprises the following steps: (1) dissolving the 15N or 18O labeled nitrate sample in a methanol aqueous solution; (2) taking the supernatant, filtering with a filter membrane, and diluting with a methanol aqueous solution to obtain a solution to be detected; and (3) detecting the solution to be detected by using a high-resolution liquid chromatograph-mass spectrometer, selecting a mass spectrum in a peak appearance time window, recording relative mass spectrum signal intensities with mass-to-charge ratios of 61.988 and 62.985, and calculating to obtain the 15N isotope abundance. Or recording the relative mass spectrum signal intensities with the mass-to-charge ratios of 61.988, 63.993, 65.997 and 68.001, and calculating to obtain the abundance of the < 18 > O isotope. Compared with the prior art, the method has the advantages that the sample pretreatment time is greatly shortened, the economic cost is low, and the test result is accurate.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention discloses a construction method of a gout compound decoction fingerprint as well as the fingerprint and application thereof, and belongs to the technical field of analysis and detection. The construction method of the fingerprint spectrum comprises the following steps: taking a test solution prepared from the gout compound decoction; the test solution is subjected to HPLC-SPD detection, so that the fingerprint spectrum of the gout compound decoction is obtained, and the HPLC detection conditions are that a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, a C18 chromatographic column is used, and a C18 chromatographic column is used for detecting the fingerprint spectrum of the gout compound decoction. An aqueous solution containing 0.1%-0.2% of phosphoric acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the flow velocity is 0.8-1 mL / min, the column temperature is 25 DEG C, the sample injection volume is 5-10 [mu] L, and a diode array detector is 190-400 nm full-wave band scanning. The chemical components in the gout compound decoction are comprehensively and systematically analyzed, and a basis is provided for quality control and in-depth study of a pharmacodynamic material basis.

The invention relates to the technical field of medicine analysis and detection, and particularly discloses a method for detecting the content of protein in a 3D-MLA adjuvant, which comprises the following steps: (1) preparing a test solution: firstly dissolving the 3D-MLA adjuvant by using a hydrochloric acid solution, performing 300-600W ultrasonic treatment for 2-5min, centrifuging 10000-13000g for 4-7min, and taking supernate; adding a compound enzyme into the supernate for enzymolysis; then adding concentrated hydrochloric acid, uniformly mixing, hydrolyzing for 20 + / -2 hours in a nitrogen atmosphere at 105-115 DEG C, blow-drying, and dissolving with a hydrochloric acid solution to obtain a test sample stock solution; adding a derivatization reagent into the test sample stock solution, and carrying out derivatization treatment, extraction and dilution to obtain a test sample solution; and (2) high performance liquid chromatography determination. According to the detection method provided by the invention, the residual quantity of the host cell protein in the 3D-MLA can be rapidly and accurately determined, and the quality control of the 3D-MLA is realized.

The invention discloses a thermal detection system and a chiral substance identification method, and relates to the technical field of micro-nano optics, the thermal detection system and the chiral substance identification method, based on the structure of the thermal detection system and the steps of the chiral substance identification method, a chiral substance to be detected is configured into a corresponding solution to be detected; a solution to be detected is sampled and placed in the absorber, the surface plasmon effect generated when the metal resonance layer receives chiral light irradiation is utilized, a corresponding thermal signal is output, then the thermal signal is captured and analyzed through the thermal detection system, and high-precision identification of chiral substances is achieved. Compared with a traditional circular dichroism and circular polarization fluorescence spectrophotometer device, the technical scheme provided by the invention is simple in structure, convenient to operate and relatively low in requirements on the concentration and the volume of a detected sample.

The invention relates to a content determination method of a traditional Chinese medicine composition fingered citron drink. The traditional Chinese medicine composition is prepared from the following medicinal raw materials: fingered citron, orange peel, malt, liquorice, Chinese date, purple perilla, citron, almond and donkey-hide gelatin. The content determination method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution and the test solution by high performance liquid chromatography; a mobile phase adopted by the high performance liquid chromatography is an acetonitrile and formic acid aqueous solution, and the elution mode is gradient elution. According to the content determination method, the content of various components can be simply, conveniently and rapidly determined, and richer chemical information and more comprehensive basis are provided for quality evaluation of the components.

This invention provides a testing device and method for corrosion and wear testing of sliding guide rails under corrosive environments. The device includes a support module, a drive module, a guide rail, a test slider, a tension sensor, a pull rod, and a test tank module. The test slider is slidably mounted on the guide rail and contains a counterweight to simulate a load. The drive module drives the pull rod to slide along the length of the guide rail. The tension sensor is connected between the pull rod and the test slider. The test tank module holds the test solution to simulate a corrosive environment. This invention features a compact overall structure, simple component installation, and a simple slider force transmission method. It can realistically simulate the corrosion and wear phenomena of the sliding guide rail moving pair under seawater conditions. Furthermore, the adjustable counterweight and replaceable test solution enable this invention to conduct corrosion and wear tests on moving pairs under different load conditions and corrosive environments.

The invention provides a traditional Chinese medicine formula granule spectrum similarity evaluation system, and relates to the technical field of traditional Chinese medicine, and the system comprises: a spectrum acquisition module, which is used for separating active components with similar polarity in a compound salvia miltiorrhiza formula granule test solution by gradient elution based on chromatographic conditions to obtain a characteristic spectrum; and the analysis processing module is used for carrying out analysis processing on overlapped chromatographic peaks in the characteristic spectrum, distinguishing characteristic signals corresponding to main active components and trace components, and realizing separation and identification of characteristic peaks of different components in the mixture so as to obtain a characteristic peak analysis result. According to the method, the standard characteristic chromatogram database is constructed through gradient elution and deconvolution processing, and the accuracy and standardization of quality evaluation of the traditional Chinese medicine formula granules are improved.

The invention provides a construction method and application of a fingerprint spectrum of a liver-warming decoction, and belongs to the technical field of medicine detection.The construction method includes the steps that a test solution is prepared through the liver-warming decoction, acetonitrile (A)-0.08-0.12 wt% formic acid aqueous solution (B) serves as a mobile phase, and the fingerprint spectrum of the liver-warming decoction is obtained through high performance liquid chromatography detection; and a standard fingerprint spectrum is established and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the liver-warming decoction. According to the construction method of the fingerprint spectrum of the liver-warming decoction, main medicinal material components in the liver-warming decoction can be detected through high performance liquid chromatography, so that the quality condition of the liver-warming decoction can be reflected more comprehensively.

The invention discloses a method for determining the content of phosphorus in activated carbon for wine, and relates to the field of detection of food processing assistants.The method comprises the steps that phosphorus standard solutions and phosphorus determination reagents are mixed to prepare phosphorus standard solutions with different concentrations, and phosphorus standard series working solutions are prepared; extracting phosphorus in the activated carbon by using aqua regia, preparing a to-be-detected solution by using the same method as the standard solution, and respectively determining absorbance of the phosphorus standard series working solution and the to-be-detected solution by using a spectrophotometer; and quantifying by adopting an external standard method, and calculating the phosphorus content in the activated carbon sample for filtering the wine. The method provided by the invention can realize accurate qualitative and quantitative analysis of the phosphorus content in the activated carbon for filtered liquor, effectively solves the problem that no standard method for detecting the phosphorus content in the activated carbon for filtered liquor exists in the liquor industry on the premise of meeting the analysis accuracy, and is simple and convenient, low in detection cost, good in repeatability of the analysis method and high in stability.

The invention discloses a separation-free and signal-amplification-free quantitative analysis method for extracellular vesicles, which comprises the following steps: assembling a sulfydryl-modified aptamer on the surface of a gold nanoparticle through an Au-S bond to form a gold nanoparticle-aptamer compound; the aptamer is designed according to a specific membrane protein receptor on an extracellular vesicle; incubating the gold nanoparticle-aptamer compound and an extracellular vesicle to-be-detected solution, and after incubation, specifically binding the gold nanoparticle-aptamer compound with EVs to form a large-particle compound; under laser irradiation, recording the Tyndall effect of the to-be-detected liquid by using a camera, and reading the Tyndall effect intensity value by using image analysis software.

The invention discloses a method for detecting an intermediate acetylglucose of tetraacetyl by an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method. The method comprises the following steps: (1) preparing a reference substance solution, namely dissolving acetylglucose by adding a diluent, and diluting to prepare the reference substance solution; (2) preparation of a test solution: taking tetraacetyl, adding a diluent solvent, and diluting to prepare the test solution; and (3) detecting the content of acetyl glucose in the test sample by adopting an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and taking octadecylsilane chemically bonded silica as a filler in an adopted chromatographic column. According to the method disclosed by the invention, the intermediate acetyl glucose of the tetraacetyl substance is detected based on an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method, and CAD parameters, a chromatographic column, a mobile phase, a diluent and a sample concentration are selected, so that the aims of effectively separating and accurately detecting the acetyl glucose in the sample can be fulfilled; the method has the advantages of simplicity, sensitivity, high accuracy, high repeatability, high specificity, good durability, high precision and the like, and has certain guiding significance on carbohydrate compound detection.

The invention discloses a benzalkonium chloride microbial limit counting inspection method, which relates to the technical field of microbial inspection, and comprises the following steps: firstly, preparing a test solution; wetting the filter membrane with the flushing fluid to obtain a rinsed filter membrane; different amounts of test solutions are taken and added into the flushing fluid, and a first mixed solution and a second mixed solution are obtained respectively; respectively filtering the mixed solution I and the mixed solution II through a rinsed filter membrane to obtain a filtered filter membrane I and a filtered filter membrane II; respectively flushing the filtered filter membrane I and the filtered filter membrane II with flushing fluid, and counting and checking the total number of aerobic bacteria through the flushed filter membrane I; counting and checking the total number of mould and saccharomycetes through the flushed filter membrane II; according to the benzalkonium chloride microbial limit counting inspection method, the application range is expanded, the total flushing amount is smaller, the flushing frequency is smaller, the error possibility in the test process is reduced, and the result is real, accurate and effective.

The invention relates to a method for detecting perfluorinated compounds in water, which comprises the following steps: carrying out solid-liquid separation on a water sample to prepare a liquid to be detected; carrying out on-line solid phase extraction-liquid chromatography-mass spectrometry detection on the to-be-detected liquid, and calculating the content of the perfluorinated compounds in the to-be-detected liquid by an internal standard method. The detection method is high in automation degree, simple and rapid to operate and capable of accurately detecting the perfluorinated compounds in the water.

The invention belongs to the technical field of medicines, and particularly relates to an HPLC (High Performance Liquid Chromatography) detection method for LXH-1211 and impurities thereof. Respectively injecting the control solution, the impurity control solution, the blank solution and the test solution into a liquid chromatograph, recording chromatograms, calculating the content of LXH-1211 in the test solution according to a peak area normalization method, and calculating the content of impurities in the test solution according to a peak area according to an external standard method; wherein the impurities are one or more of ZZ1, ZZ2, ZZ3 or ZZ4. The method is good in reproducibility and stability and excellent in precision.

The invention discloses a CAD detection method for phosphoric acid and phosphorous acid in an alendronate sodium oral solution, and belongs to the technical field of pharmaceutical analysis. The method comprises the following steps: preparing a reference stock solution and a test solution; then setting high performance liquid chromatography detection conditions; a Shodex VN-504D chromatographic column is adopted, a CAD detector is adopted as a detector, a mixed solution of a mobile phase A and a mobile phase B is adopted as a mobile phase, the mobile phase A is ammonium formate, the mobile phase B is an acetonitrile solution, and gradient elution is performed by adopting the mobile phase; and respectively measuring the reference stock solution and the test solution, injecting into a liquid chromatograph, and recording a chromatogram. A CAD detector is adopted, the detection sensitivity is obviously improved, the quantitative concentration can reach 0.24 mu g / mL, and the detection concentration can reach 0.10 mu g / mL. The method is high in specificity, high in sensitivity, good in durability, rapid, simple, effective and reliable.

The invention discloses a method for detecting the purity of a phosphorus-containing flame retardant, and belongs to the technical field of flame retardant detection. The specific test method comprises the test steps of selecting a solvent, preparing a to-be-tested solution, determining the maximum absorption wavelength, determining absorbance, calculating the purity of a sample and the like, and analyzing and processing an experimental result. The solvent is filtered through a 600-mesh screen, the influence of impurities in the solvent on an experimental result is eliminated, and the scientificity of the experimental result is ensured by parallelly determining the average value recorded for three times; the testing method has the advantages of low cost, simplicity and convenience in operation, short detection period and the like, and can realize rapid detection of a plurality of samples based on multiple stations of an ultraviolet-spectrophotometer, so that the working efficiency is improved; meanwhile, the method for detecting the purity of the phosphorus-containing flame retardant provided by the invention can be used for comparing the quality of products from different manufacturers and the batch stability of the products at the same time, and is beneficial for enterprises to control the quality of the products.

The invention discloses a method for determining the content of bis (2-aminophenyl) disulfide in 2-aminothiophenol. The method comprises the following steps: preparing an acetonitrile solution of citric acid as a diluent; preparing a system applicability solution, a reference solution and a test solution by using a diluent; a diluent, a system applicability solution, a reference substance solution and a test solution are sequentially injected by adopting a liquid chromatography, and the content of bis (2-aminophenyl) disulfide in a 2-aminothiophenol sample is determined by adopting an external standard method. The antioxidant citric acid is added into the diluent, so that the generation of the disulfide compound is inhibited, the existing disulfide compound is not damaged, the solution stability is high, the operation is simple, the system applicability, specificity, accuracy, precision and durability completely accord with the guidance principle verified by the Chinese pharmacopoeia method, and the method is suitable for industrial production. The method can be used for quality control of 2-aminothiophenol.

The invention discloses a thin-layer chromatography rapid identification method for vigor-preserving decoction particles, and aims to solve the problems of complicated operation, more interference, low efficiency and the like of the existing method. According to the method, a test solution is prepared through methanol extraction and water-saturated n-butyl alcohol and ammonia test solution extraction, reference medicinal materials and seven reference solutions are matched, a petroleum ether-ethyl acetate-methanol-formic acid mixed solution is adopted as a developing solvent, and inspection is conducted after developing is conducted through a silica gel H thin-layer plate. The method can be used for accurately identifying seven key components in the vitality-preserving decoction granules at one time, is high in specificity, simple and convenient to operate, environment-friendly and high in efficiency, and provides rapid and reliable technical support for quality control of the vitality-preserving decoction granules.

The invention discloses a thin-layer identification method of a nux prinsepiae aqueous extract, the thin-layer identification method comprises the following steps: preparing a reference medicinal material solution and a test solution, the test solution comprises a formula granule test solution and a standard decoction test solution, respectively dropping the reference medicinal material solution and the test solution on the same thin-layer plate, ethyl acetate-methanol-formic acid is used as a developing solvent for developing, a color developing agent is sprayed for color development, and a multi-information nux prinsepiae thin-layer chromatogram is obtained. The method is easy and convenient to operate, good in chromatographic spot separation degree, low in detection cost, environmentally friendly and suitable for identifying the nux prinsepiae aqueous extract.

The invention belongs to the technical field of geological sample analysis and detection, and particularly discloses a method for measuring the amount of each element of lithium ore in a closed acid-soluble system, and the method comprises the following steps: pretreating the lithium ore; the pretreated lithium ore is weighed and put into a closed sample dissolving device, hydrofluoric acid and purified nitric acid are sequentially added, and the closed sample dissolving device is closed; carrying out heat preservation on the closed sample dissolving device twice to obtain a test solution; and measuring the test solution by using an inductively coupled plasma mass spectrometer to obtain a working curve, and calculating the content of the analysis element in the sample solution by using the working curve. The method for determining the amount of each element in the lithium ore by adopting the closed acid-soluble system has the characteristics of simplicity in operation, high efficiency and good stability, realizes simultaneous determination of the amount of lithium, rubidium, cesium, beryllium, strontium, niobium, tantalum, zirconium, hafnium and rare earth elements in the large-batch lithium ore, and further provides a reference for determination of main trace elements in the lithium ore.

The invention belongs to the technical field of chemical component detection, and particularly relates to a method for detecting residues of 1, 3-dichloro-2-propanol, 2, 3-dichloro-1-propanol and 3-chloro-1, 2-propylene glycol in dihydroxypropyl theophylline. The detection method comprises the following steps: (1) adding a solvent into diprophylline for dilution, and uniformly shaking to prepare a test solution; (2) detecting the liquid to be detected by adopting a gas chromatography-tandem mass spectrometry method, and calculating the content of 1, 3-dichloro-2-propanol, the content of 2, 3-dichloro-1-propanol and the content of 3-chloro-1, 2-propylene glycol in the diprophylline according to an external standard method; the solvent is a mixture of formic acid and DMF (Dimethyl Formamide). The method provided by the invention is good in specificity, high in sensitivity, good in repeatability and high in accuracy, and can be used for simultaneously detecting 1, 3-dichloro-2-propanol, 2, 3-dichloro-1-propanol and 3-chloro-1, 2-propylene glycol in the diprophylline.

The invention relates to the technical field of environmental monitoring, in particular to a method for determining two organophosphate compounds in water. The two organic phosphate ester compounds are tri-(2, 3-dibromopropyl)-phosphate ester and xylenol phosphate ester; the method comprises the following steps: 1, putting a water sample to be detected into a centrifugal tube, adding sodium chloride, uniformly mixing, adding acetonitrile as an extracting agent, uniformly mixing by vortex, standing for layering, and filtering supernatant liquid by a needle filter to obtain liquid to be detected; 2, mixing the prepared tri-(2, 3-dibromopropyl)-phosphate standard intermediate solution with the prepared xylenol phosphate standard intermediate solution, and diluting with acetone to obtain a standard working solution; the to-be-detected liquid obtained in the first step is placed in a liquid chromatography-triple quadrupole mass spectrometry combined system to be analyzed; the method provided by the invention can realize simultaneous determination of tri-(2, 3-dibromopropyl)-phosphate and xylenol phosphate, and fills the blank of combined detection of two specific organophosphate compounds in the prior art.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a method for determining the content of five flavonoid components in a hypericum perforatum medicinal material. The content determination method comprises the following steps: preparing rutin, hyperoside, isoquercitrin, quercitrin and quercetin into a mixed reference solution; extracting a hypericum perforatum medicinal material by using an extraction solvent to prepare a test solution; the mixed reference substance solutions and the test solution with different volumes are subjected to high performance liquid chromatography respectively, a mobile phase A is acetonitrile, a mobile phase B is a phosphoric acid aqueous solution with the volume concentration of 0.08-0.12%, and elution gradient is adopted. The content determination method has the advantages of high sensitivity, simplicity and convenience in operation, low cost and high detection speed.

The invention relates to the field of detection, in particular to a method for detecting phenolic stine compounds in food, which comprises the following steps: taking a sample in a centrifugal tube, and adding methanol for dissolving to obtain a sample solution; target ultrasonic parameters are determined according to the response surface model, and ultrasonic extraction is carried out according to the target ultrasonic parameters; centrifuging the sample solution subjected to ultrasonic extraction; centrifuging, taking supernate, and filtering to obtain a solution to be detected; preparing a standard series of working solutions; and detecting the sample solution and the standard series of working solutions by using a liquid chromatography-tandem mass spectrometer to obtain the content of the phenolic stine compounds in the sample. According to the application, the response surface model can determine the highest recovery rate of the sample after the sample is treated by the ultrasonic parameters, so that the sample is subjected to ultrasonic treatment according to the determined ultrasonic parameters, and the phenol-tin in the sample can be fully released into the solution; and thus, a more accurate detection result can be obtained when the solution is detected.

The invention relates to the field of analysis and detection, and discloses a method for detecting multiple chelating agents in daily chemical products, which comprises the following steps: methylating a to-be-detected sample by using a diazomethane methylation reagent to obtain a to-be-detected solution; carrying out liquid chromatography-mass spectrometry detection on the to-be-detected liquid by adopting a specific elution program to obtain a chromatogram; and performing qualitative and / or quantitative analysis on the chelating agent in the to-be-detected sample according to the chromatogram. By adopting the detection method disclosed by the invention, efficient detection of various chelating agents (including DTPA, DTPMP, EDTMPA, HDTEPA, EGTA, HEDP, EDTA, NTA, GLDA, PBTC, MGDA, IDS, HEEDTA, HPAA, DTPA-OH, CA and ATMP) can be realized, meanwhile, interference of other components in daily chemical products on detection can be avoided, and relatively high detection sensitivity and accuracy of the chelating agents can be realized.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for rapidly identifying non-volatile components in ledum palustre and a preparation thereof and application, the method comprises the following steps: taking a ledum palustre sample, adding an extraction solvent A, soaking, boiling, distilling, filtering, concentrating an extracting solution, and freeze-drying to obtain ledum palustre A extract freeze-dried powder; drying and crushing filter residues, adding an extraction solvent B, performing reflux extraction, recovering the solvent B, and performing freeze drying to obtain ledum palustre B extract freeze-dried powder; respectively taking the ledum palustre A extract freeze-dried powder and the ledum palustre B extract freeze-dried powder, adding an extraction solvent C, carrying out ultrasonic and centrifugal treatment, and taking supernate to obtain a test solution; injecting the test solution and the reference solution into an ultra-high performance liquid chromatograph and a high-resolution mass spectrometer for determination; and analyzing the collected data. 118 non-volatile chemical components are identified, 102 non-volatile chemical components are unreported components in ledum palustre, and reference is provided for ledum palustre pharmacodynamic substance research, quality control and product development.

The invention provides a method for detecting the contents of collagen and regenerated silk fibroin in a composite material, which comprises the following steps: (1) hydrolyzing the composite material for a certain time, and neutralizing to obtain a solution to be detected; (2) preparing a tyrosine reference solution; and (3) detecting the peak areas of the solution to be detected and the tyrosine reference substance solution by adopting high performance liquid chromatography, and calculating to obtain the content of regenerated silk fibroin in the composite material, and (4) detecting the total nitrogen content of the composite material by using a Kjeldahl method, and calculating to obtain the collagen content in the composite material. According to the method, the high performance liquid chromatography is combined with the Kjeldahl determination method, accurate determination of two components can be realized without derivatization treatment, the operation is simple and convenient, and the detection cost is low; the limit of quantitation of the method reaches 10.35 mu g / mL (S / N is greater than or equal to 16.59), the precision RSD is less than 1%, and the method has high sensitivity and accuracy.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a Kangfugu anti-inflammatory suppository characteristic spectrum, which comprises the following steps: 1, preparing a reference substance solution; 2, preparing a test solution; 3, determining conditions; and 4, importing the chromatogram into a traditional Chinese medicine fingerprint spectrum to generate a control characteristic spectrum. According to the characteristic method, 13 characteristic peaks are defined, the method is good in repeatability and high in precision, the chemical component characteristics of the Kangfugu anti-inflammatory suppository are fully and integrally displayed, and the medicine quality of the Kangfugu anti-inflammatory suppository is integrally controlled.