The invention discloses a method for preparing low molecular weight holothurian
polysaccharide. Sea cucumbers are cleaned, minced, heat-gelatinated, freeze-dried, coarse ground,
airflow crushed and nanometer crushed to produce nanometer
sea cucumber powder, then prolease is used to perform 1 to 3 times of enzymolysis, and then a low molecular weight holothurian
polysaccharide solution is obtained through the
centrifugation; and then a crude low molecular weight holothurian total
polysaccharide product is obtained through salt-adding
alcohol precipitation and
centrifugation, and finally is subjected to
hydrogen peroxide decolorization, salt-adding
alcohol precipitation and centrifugal
drying to obtain the low molecular weight holothurian polysaccharide. The obtained crude low molecular weight holothurian total polysaccharide product is dissolved, is added with
potassium salt and precipitates a holothurian glycosaminoglyan precipitate at low temperature, a supernatant fluid is a holothurian fucoidin solution, and the holothurian glycosaminoglyan precipitate and the holothurian fucoidin solution are separated through the
centrifugation, and are decolored respectively. Low molecular weight holothurian fucoidin is obtained through salt-adding
alcohol precipitation, separation and
drying; and the
potassium salt is added to precipitate at low temperature, and low molecular weight holothurian glycosaminoglyan is obtained through separation and
drying. The method utilizes gelatinated nanometer sea cucumbers to directly perform the low molecular weight polysaccharide, and the low molecular weight polysaccharide has low molecular weight, has good absorption of human bodies, greatly reduces the dosage, and obviously strengthens the effect.