The invention relates to a formula of a Chinese medicine for preventing and treating chemotherapeutic toxic and side reaction and a preparation method for Chinese medicinal pills. The Chinese medicine consists of donkey-hide gelatin, ginseng, cassia bark, Szechuan lovage rhizome, prepared rehmannia root, tuckahoe, largehead atractylodes rhizome, astragalus, Chinese angelica, white paeony root, south dodder seed, medlar, medicinal cyathula root, tangerine peel, glossy ganoderma, desertliving cistanche, hairy antler, musk, stalactite, medicinal indianmulberry root, prepared common monkshood daughter root, sea cucumber, liquoric root, deerhorn glue and yellow wine in a certain weight ratio. The preparation method comprises the following steps of: weighing the deerhorn glue in the weight ratio, adding into the yellow wine in the weight ratio, decocting, and cooling to room temperature to obtain glue solution; weighing the rest medicinal materials in the weight ratio, mixing uniformly, and crushing to form medicinal powder; and adding the medicinal powder into the glue solution, mixing uniformly, and pelletizing to form pills, wherein each pill is 5 grams. Clinical tests prove that the Chinese medicine can prevent and treat chemotherapeutic toxic and side reaction safely and effectively; and the preparation method for the pills is simple, convenient, easy and environment-friendly, and is suitable for production and clinical application.