The invention discloses a preparation method and application of sheep brain cephalin and
lecithin. The method comprises the following steps: washing a fresh sheep brain to
remove blood stains and impurities from the surface, freeze-
drying the brain, crushing the freeze-dried sheep brain, sieving the brain, adding an
organic solvent for extraction by stirring, combining the extracts, filtering the brain, and naturally air-
drying the precipitate until the
organic solvent is completely volatilized; concentrating the filtrate, adding
anhydrous ethanol for
reflux, filtering the filtrate, allowing the filtrate to stand overnight until stratification, filtering the filtrate, concentrating the filtrate in a vacuum or freeze-
drying process to obtain
lecithin; adding
acetone for
cholesterol removal to obtain pure
lecithin; adding
calcium chloride to the precipitate, stirring and extracting the precipitate, and centrifuging the precipitate to obtain a crude cephalin extract; extracting the crude extract by stirring with
anhydrous ethanol, discarding the supernatant, dissolving the precipitate with
ethanol, filtering the precipitate, and vacuum-drying or freeze-drying the precipitate to obtain a refined cephalin product. The method is simple, low-cost, efficient, and has a short extraction time and simple operation. The method provides raw materials and
technical support for the development of drugs, preparation of health products, or application in the field of
cosmetics based on active compounds such as lecithin and cephalin.