The invention discloses a method for ultra-low temperature preservation and detoxification of
pinellia ternata, comprising material culture, loading treatment,
vitrification treatment, ultra-
low temperature treatment, thawing and warming treatment, unloading treatment and regeneration
plant culture. The
pinellia ternata
vitrification ultra-low temperature preservation
technology system established by the invention can be applied to the preservation of
pinellia ternata
germplasm resources. Compared with field preservation and delayed growth preservation technology, the technology can greatly save manpower and
material resources, is easy to operate, is not easily attacked by natural disasters, does not need to transfer
plant materials regularly, occupies a small space, has low preservation cost, long preservation time, is genetically stable, and can preserve
pinellia ternata germplasm for a long time; the technology can also be applied to the detoxification of
pinellia ternata plants. After detoxification of
test tube tubers of second-generation diseased plants of
pinellia ternata, it was found through experimental
verification that the detoxification rate of
soybean mosaic virus (SMV) was 85.7%, and the detoxification rate of
cucumber mosaic virus (CWV) was 57.1%, and the detoxification effect was better than that of commonly used heat treatment and stem tip detoxification.