The invention relates to the technical field of
biochemical detection, in particular to a composite surfactant composition based on a synergistic effect and a preparation method and application of the composite surfactant composition. The composite surfactant composition comprises a Tween emulsifier, an OP emulsifier and a
polyoxyethylene alkyl ether emulsifier, and the Tween emulsifier can promote mutual
dissolution of the OP emulsifier and the
polyoxyethylene alkyl ether emulsifier through
hydrogen-bond interaction; a uniform
micelle structure with a
small particle size is formed, the
turbidity of a
system is reduced, a long-chain hydrophobic group of the
polyoxyethylene alkyl ether emulsifier can enhance the anchoring capacity to
chylomicron and cooperates with a hydrophilic
chain segment of the OP emulsifier, so that the lipid dispersion efficiency is improved, the interference inhibition rate is larger than or equal to 60%, and the stability of the
system is improved. Meanwhile, after the Tween emulsifier, the OP emulsifier and the polyoxyethylene
alkyl ether emulsifier are compounded,
chyle particles can be efficiently wrapped,
light scattering interference is reduced, and the anti-lipid interference efficiency is improved by more than or equal to 95% compared with that of a
single component, so that the effect of eliminating interference in a lipid /
chyle blood sample by adopting the composition is better.