123 results about "Biochemical detection" patented technology

The present application relates generally to the field of biochemical detection. The invention provides a method and a kit for detecting an anti-IgE antibody in a biological sample. The method for detecting the anti-IgE antibody in the biological sample comprises the following steps: (a) contacting the biological sample with an acidolysis solution to dissociate the anti-IgE antibody from IgE so as to obtain an acidolysis sample; (b) contacting the acidolysis sample with a neutralization buffer solution to terminate acidolysis so as to obtain a to-be-detected sample; and (c) determining the anti-IgE antibody in the sample to be detected. The kit for detecting the anti-IgE antibody in the biological sample comprises an acidolysis solution and a neutralization buffer solution. The problem of background IgE interference in anti-IgE antibody detection is solved to a certain extent.

The utility model belongs to the technical field of biochemical analyzers, in particular to a probe cleaning device for a full-automatic biochemical analyzer, which comprises a loading plate, supporting legs are fixed at four corners of the bottom of the loading plate, a cleaning box is mounted at the top of the loading plate, and a water distribution pipe is mounted in the cleaning box; according to the utility model, through cooperative use of the installation pipe, the through hole, the elastic sheet, the fixed block, the limiting sheet and the sleeve, rapid positioning and stable fixation of the probe are realized, so that the purpose of synchronous cleaning of the inner wall and the outer wall is achieved, and the efficiency of the cleaning process and the thoroughness of probe cleaning are significantly improved. By means of the design, the cleaning process is more efficient and convenient, and an operator can easily master the cleaning device due to the simple structure. The device is low in maintenance cost, has relatively high economic applicability, and is suitable for being popularized and applied to various biochemical analyzers. By using the device, sample pollution caused by incomplete cleaning can be effectively avoided, so that the accuracy and reliability of biochemical detection data are ensured.

This invention belongs to the field of biochemical detection and discloses a portable hepatitis biomarker detection kit. The kit contains a hydrogel; the hydrogel is obtained by dispersing hollow manganese dioxide in water to obtain an H-MnO2 dispersion, mixing the H-MnO2 dispersion with sodium alginate solution to obtain a mixed solution, and then adding a calcium salt solution to obtain the hydrogel. The hollow manganese dioxide is obtained by etching manganese Prussian blue analog nanoparticles with sodium hydroxide. The manganese Prussian blue analog nanoparticles are prepared from potassium ferricyanide, polyvinylpyrrolidone, and manganese salts. This invention utilizes the color change of hollow manganese dioxide in relation to TMB to construct a method for detecting hepatitis biomarkers using a smartphone to capture signals. This kit reduces the detection cost of hepatitis biomarkers, does not require large instruments, and is highly portable and widely applicable, possessing great potential as a novel point-of-care testing device.

The invention firstly comprises a junction field effect transistor (JFET), namely a SiC JFET, which is completely made of SiC. The transistor is preferably manufactured by adopting a top-down method, and the manufacturing process is suitable for large-scale production. The electrical properties of the proposed SiC JFET have been evaluated under dry conditions and in a liquid medium. The concept feasibility of the SiC JFET as a biosensor 0 is verified through pH value measurement. The sensitivity of the SiC JFET provided by the invention is up to 495 mV / ph, that is, the sensitivity is nearly 100 times higher than the theoretical Nernst limit. Therefore, the device is suitable for various in-vitro and in-vivo biochemical detection applications because the device meets performance indexes generally required in the field, such as sensitivity and chemical stability, especially long-term stability.

1. The name of the design product: colorimetric cup. 2. The use of the design product: for biochemical detection instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention relates to a general butt joint module for a production-free integrated assembly line, and relates to the field of medical instruments. Comprising a bottom plate, a portal frame, a test tube transfer manipulator assembly, a universal transfer trolley for butt joint of biochemical sample racks and a universal transfer trolley for butt joint of immune sample racks, a biochemical sample rack universal temporary storage position is arranged in the middle of the bottom plate; immune sample rack universal temporary storage positions are arranged on the two sides of the bottom plate; a track I and a track II are respectively arranged on two sides of the bottom plate, the universal transfer trolley for butting the biochemical sample frame is arranged on the track I in a sliding manner, and the universal transfer trolley for butting the immune sample frame is arranged on the track II in a sliding manner; the portal frame is mounted on the bottom plate, and the test tube transfer manipulator assembly is mounted on the portal frame and is used for accurately carrying samples needing biochemical detection in the immune sample frame into the biochemical sample frame. The device is exquisite in design, can effectively coordinate differences of biochemical and immune sample racks in the aspects of tracks and hole numbers, and realizes smooth connection and efficient transmission of the sample racks.

The invention belongs to the technical field of biochemical detection, and particularly relates to a detection kit, system and method for light-chain monoclonal immune globulin based on fluorescence labeling. According to the method, whether the light chain type monoclonal immune globulin exists or not and the concentration of the light chain type monoclonal immune globulin are determined by reducing and specifically marking free sulfydryl at the C tail end of a free light chain of the monoclonal immune globulin in a sample and combining electrophoresis and a fluorescence detection system. The detection rate of the light-chain monoclonal immune globulin is increased, the cost is low, the operation is simple and convenient, the method is suitable for large-scale screening and grassroots scenes, and the popularization of the light-chain monoclonal immune globulin detection technology is promoted.

The application relates to an intelligent control method and system of high-pressure atomization irrigation and a storage medium thereof, and aims to solve the problem that traditional water and fertilizer management of high-value crops cannot realize real-time sensing of internal physiological states of crops, leading to inconsistent quality. The intelligent control method divides an irrigation area into multiple management partitions, randomly selects a plurality of sacrifice samples in each management partition, destructively samples the sacrifice samples, obtains true value data of crops through physiological and biochemical detection, establishes and dynamically updates an irrigation rule library based on the true value data, generates variable control instructions for each management partition in combination with real-time environmental data, and finally controls a high-pressure atomization sprinkler irrigation system to accurately execute. The application also provides a system and a storage medium for realizing the method, realizes accurate regulation and control starting from the real demand of crops, effectively improves the consistency of crop quality and the resource utilization efficiency, and has self-learning and predictive prevention and control capabilities.

The utility model relates to the technical field of biochemical detection, and discloses a multi-station mechanism for rapid detection of biological reagents, which comprises a base, a motor I is fixedly connected in the base, a rotating shaft I is fixedly connected to the output end of the motor I, a turntable is fixedly connected to the outer wall of the rotating shaft I, a placement hole I is formed in the turntable, and a placement hole II is formed in the placement hole I; a clamping assembly is arranged on the upper surface of the rotating disc. The clamping assembly comprises a first threaded rod, the lower end of the first threaded rod is rotationally connected to the upper surface of the rotary disc, the driving assembly comprises a telescopic rod, the lower surface of an electric telescopic rod is fixedly connected to the upper surface of the base, and the output end of the electric telescopic rod is fixedly connected with a supporting rod. According to the utility model, test tubes with different specifications can be clamped and fixed through the matching of the test tube clamp and the positioning frame, a plurality of placing holes can be used for detecting at the same time, and mixed reagents can be better and uniformly mixed by the turntable to achieve better detection.

The invention provides a nucleic acid composition and a kit for identifying a rice variety rice flower No.2, and relates to the technical field of biochemical detection. The nucleic acid composition comprises a specific amplification primer pair and a specific detection probe. The nucleic acid composition locks the specific SNP site of the rice, and by utilizing the synergistic effect of the primer and the double probes, the rice flowery No.2 variety and the similar variety can be accurately distinguished, and the doping can be sensitively identified. The method is suitable for a real-time fluorescent PCR platform, electrophoresis and sequencing are not needed, rapid and high-sensitivity closed tube detection is achieved, and an efficient means is provided for authenticity identification and purity analysis.

The present disclosure provides a time sequence control method for a dry biochemical analyzer, a control apparatus, and a storage medium. The method comprises: dividing one biochemical test into k time sequence control cycles each in the form of nA+B, wherein each time sequence control cycle consists of two parts: nA representing n light measurement preparation parts, and B representing one light measurement part, and multiple reagent card tests are performed in each time sequence control cycle; in a light measurement preparation part of a current time sequence control cycle, controlling a card feeding device to perform card feeding actions required for light measurement on n reagent cards in the current time sequence control cycle, and controlling a sample loading device to perform sample loading actions required for light measurement on n reagent cards in the previous time sequence control cycle; and according to a preset light measurement action required by a biochemical test method in each time sequence control cycle, controlling, in a light measurement part of each time sequence control cycle, a light measurement mechanism to perform light measurement actions on all reagent cards in a reagent card reaction device. The total test duration of multiple items or multiple reagent cards is shortened, thereby improving the test efficiency.

The utility model relates to a sample analyzer and relates to the technical field of biochemical analysis. The sample analyzer provided by the utility model comprises an optical module for performing optical detection on a sample reagent mixed solution in the cuvette. The optical module comprises a light source unit, the light source unit comprises a light source, the wavelength of light emitted by the light source can cover the wavelength required by biochemical detection, the wavelength of light emitted by the light source can cover the wavelength required by biochemical detection, and the light source comprises a plurality of light-emitting elements. The light emitted by the plurality of light-emitting elements jointly forms the light of each wavelength required by the detection unit; the sample analyzer further comprises a colorimetric assembly, and the colorimetric assembly is used for matching the sizes of front and rear light spots.

The utility model relates to the technical field of biochemical detection, in particular to an optical detection device for biochemical detection, which is characterized in that dismounting and replacing mechanisms are arranged on the inner wall of a fixed block and the surface of a detection tube, and clamping and fixing mechanisms are arranged on the inner wall of a test tube rack and the surface of a test tube body; and convenient adjusting mechanisms are arranged in the supporting table and on the surface of the moving plate. According to the utility model, when the optical detection device is used, the test tube body can be clamped and fixed on the surface of the test tube rack according to the size of the test tube body, so that the optical detection device is prevented from inclining during detection; according to the optical detection device, the positions of the test tube rack and the test tube body can be adjusted according to the position of the detection tube when the optical detection device is used, so that the detection effect of the optical detection device is better, a user can conveniently take and place the test tube body, and the user can conveniently take down the detection tube from the surface of the fixed block when the optical detection device is used; and a user can use the device conveniently.

Smell loss (hyposmia) is a common cognitive deficit perceived clinically as a nasal abnormality but, in reality, it is a deficit of complex biochemical detection and processing of externally generated chemical events. A major mechanism initiating hyposmia involves abnormalities of growth of human olfactory cells through altered growth factor stimulation of stem cell activity. While several growth factors are involved well known signaling substances generate and perpetuate this process; among these substances are cAMP and cGMP. We have hypothesized a role for cAMP in taste and olfaction as a growth factor necessary for stem cell maturation. We have thus measured cAMP and cGMP in parotid saliva of 19 normal subjects and 144 patients with hyposmia to evaluate roles for these signals in cognition. Results indicate that patients secrete significantly less cAMP (0.40+ / −0.03 pmol / mg protein (Mean+ / −SEM); patients; 0.67+ / −0.09, normals, p<0.005) and significantly less cGMP (0.072+ / −0.003, patients; 0.100+ / −0.007, normals, p<0.005) than do normals. While parotid gland secretions relate directly to taste bud growth factors many substances secreted in saliva are similar to those secreted in nasal mucus. The present results indicate aberrant signaling in saliva may serve both as a marker for hyposmia and as an indicator of a mechanism which interferes with olfactory system function.

This invention discloses a method and system for simultaneous analysis of multiple lipid indicators in fingertip capillary blood, relating to the field of chemical analysis. The method includes S1: micro-sample pretreatment of fingertip blood; S1.5: sample matrix interference pretreatment; S2: multi-channel parallel specific reaction; S3: multi-band spectral synchronous detection; S4: machine learning intelligent calibration and analysis; and S5: result output and quality control verification. This method and system achieves simultaneous detection of five core lipid indicators in fingertip blood samples in a short time, with low deviation and cross-interference rates between the detection results and venous blood biochemical detection, and high matrix interference inhibition rate. It also possesses advantages of portability, speed, and accuracy, solving the problems of low detection efficiency, limited applicability, and matrix interference affecting accuracy in existing technologies.

The application discloses a biochemical detection device and belongs to the technical field of rapid diagnosis. The biochemical detection device comprises a substrate and is provided with a liquid channel, a calibration channel and a gas channel on the substrate. The liquid channel comprises a liquid inlet channel and a reaction channel which are communicated with each other. The calibration channel is communicated with the reaction channel, and calibration reagent can flow into the reaction channel through the calibration channel. The gas channel is communicated with the liquid inlet channel, and gas can drive the liquid in the liquid inlet channel to flow to the reaction channel through the gas channel. A detection test paper is arranged on the reaction channel. The gas channel and the liquid channel are communicated with each other, so that the sample can conveniently flow from the liquid inlet channel to the reaction channel under the driving action of the gas. The sample flow is driven in a pneumatic mode, and the operation is simple and convenient. Meanwhile, the manufacturing process of the biochemical detection device is simplified, and the cost is reduced.

The application discloses a reflective terahertz metasurface sensor, a biochemical detection system and application, and the sensor comprises a resonance unit with a preset period length, and the resonance unit comprises, from top to bottom, a first gold layer, a first chromium layer, a polyimide material layer, a second gold layer, a second chromium layer and a silicon dioxide material layer; wherein the width and length of the first gold layer have a preset ratio, the first gold layer and the second gold layer have a first preset thickness, and the polyimide material layer has a second preset thickness, and the application has the advantages of improved detection precision, effective differential detection of trace biochemical samples and the like.

This utility model relates to the field of biochemical detection technology, and discloses a test tube rack and a sampling system. The test tube rack includes a test tube rack body with several insertion hole units for placing test tubes. Each insertion hole unit includes outer insertion holes arranged opposite each other and two rows of inner insertion holes arranged inside the outer insertion holes. The outer and inner insertion holes are spaced apart along their length and are staggered. A first detection slot is provided on the outer circumferential surface of each outer insertion hole, and a second detection slot is provided on the outer circumferential surface of each inner insertion hole. A gap is provided between adjacent outer insertion holes, and the gap communicates with the second detection slot. An elastic clamping structure is provided on the insertion hole unit.

The invention relates to a radio frequency resonance sensing type fingerstall for quantitative biochemical detection, comprising: a fingerstall assembly comprising a first fingerstall, a second fingerstall, and a connecting part for connecting the first fingerstall and the second fingerstall, the first fingerstall and the second fingerstall being respectively used for sleeving a thumb and an index finger; the quantitative ring cutting device is arranged on the surface of the finger prominence position of the first fingerstall; the radio frequency resonance sensor is arranged on the surface of the finger prominence position of the second fingerstall; the radio-frequency signal acquisition circuit module is arranged at the finger root of the second fingerstall, is electrically connected with the radio-frequency resonance sensor and is configured to be used for transmitting a sweep-frequency radio-frequency excitation signal to the radio-frequency resonance sensor and receiving a reflected signal of the sweep-frequency radio-frequency excitation signal; when the thumb sleeved with the first fingerstall and the index finger sleeved with the second fingerstall stretch into the two sides of the sheet-shaped part of the plant to be detected and are kneaded, a sample with the fixed size is cut from the sheet-shaped part of the plant to be detected through the quantitative ring cutting device, and the cut sample directly makes contact with the surface of the radio frequency resonance sensor; therefore, the resonant frequency of the radio frequency sensor is shifted and / or the reflection coefficient is changed, so that the reflection signal of the radio frequency resonant sensor received by the radio frequency signal acquisition circuit module is changed. Thus, the flexible wearable fingerstall provided by the invention integrates an unmarked, non-modified and direct contact type radio frequency resonance sensor and a quantitative ring cutting device for quantitative sampling, can perform quantitative sampling and real-time detection on solid samples such as plant leaves and the like, and realizes an integrated operation process of'detection once pinching '; and the standardization, portability and field applicability of detection are remarkably improved.

The application discloses a kind of lipoprotein detection reagent and lipoprotein detection method, it is related to biochemical detection technical field, the lipoprotein detection reagent includes R1 reagent and R2 reagent, wherein, the R1 reagent includes anti apolipoprotein B antibody, the R2 reagent includes latex microsphere coated with lipoprotein specificity antibody. Wherein, anti apolipoprotein B antibody can preliminarily enrich target lipoprotein and form immune complex to amplify detection signal, and lipoprotein specificity antibody can specifically recognize target protein and form immune complex-lipoprotein specificity antibody-latex microsphere complex, further amplify detection signal, significantly improve the detection sensitivity of reagent.

The invention provides a method for screening and cultivating multi-substrate degrading bacteria and algae resistant to an extreme environment. The method comprises the following steps: sampling at least two habitats from an industrial wastewater pool, a compost fermentation heap and saline-alkali soil, and pretreating to obtain an initial bacteria and algae suspension; a composite carbon source culture medium containing cellulose, lignin and fat is adopted, a salinity, pH and temperature multi-dimensional gradient environment is set, and finally the culture medium is transferred to a more extreme culture medium for domestication; diluting the enriched bacteria and algae groups, injecting the diluted enriched bacteria and algae groups into a micro-fluidic chip containing a fluorescence labeled substrate, sorting single cells with the fluorescence decay rate of more than or equal to 80% by using a flow cytometer, and performing inoculated culture; extracting DNA for sequencing, and combining physiological and biochemical detection and product analysis to obtain potential bacteria and algae; and after compound mutagenesis, inoculating to a reactor to gradually increase the substrate concentration for domestication. According to the method disclosed by the invention, through an integrated technical chain of multi-source sampling pretreatment, carbon source gradient enrichment, high-throughput screening, multi-dimensional identification and directional breeding, high-activity stable bacteria and algae resources which are resistant to an extreme environment and can synchronously degrade various organic matters are rapidly obtained.

The invention belongs to the technical field of biological medicine, and provides a human umbilical cord mesenchymal stem cell preparation and application thereof, the preparation comprises human umbilical cord mesenchymal stem cells, ginsenoside Rg3 and a medicinal carrier; the medicinal carrier is a phosphate buffer solution; according to the application, 1 * 10 < 6 > HUCMSCs pretreated by Rg3 are subjected to caudal vein injection on a T2DM mouse model induced by high fat diet combined with STZ, the curative effect and mechanism are verified by combining OGTT, IPITT, serum biochemical detection, HE / PAS tissue staining, q-PCR and other technologies, and finally the purposes of improving glycometabolism of the T2DM mouse, repairing liver / kidney / pancreas target organ injuries, protecting pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions, reducing pancreas islet beta cell functions and the like are achieved. And a theoretical basis is provided for accurate intervention of T2DM by activating an IGF-1R / PI3K-AKT signal channel and regulating and controlling the expression of a glycometabolism-related gene.

A chip processing device integrated with a reagent kit, a gene sequencer, a gene sequencing apparatus, and a method of performing biochemical detection. The chip processing device includes a substrate extending in a first direction, and a reagent kit platform and a chip platform assembled side-by-side and adjacently on the substrate in a second direction transverse to the first direction. The chip platform has a chip receiving area for accommodating a chip, the reagent kit platform has an accommodating chamber, and the reagent kit is received in the accommodating chamber. The reagent kit has a first fluid transport structure, the chip platform has a second fluid transport structure located on the chip platform and at least partially overlap and communicate with the first fluid transport structure. The first fluid transport structure is in fluid communication with the chip via the second fluid transport structure.

The application belongs to the field of biochemical detection, and particularly relates to application of HNRNPA2B1 gene expression level detection substance in postpartum depression detection and a detection system. The application of the HNRNPA2B1 gene expression level detection substance in the preparation of a postpartum depression detection product, wherein the HNRNPA2B1 gene expression level detection substance comprises primers or a gene detection chip. The sequence of the primers is as follows: a forward primer: 5'-TAGAGCACTGAGTGCAGTAACG-3', and a reverse primer: 5'-CAGTAAGCTAGATCCACTGACGT-3'. The postpartum depression detection reagent provided by the application is applied to a postpartum depression detection system, and the detection system shows that the HNRNPA2B1 detection precision is more than 0.9, which is high precision, and is very suitable for being used as a postpartum depression early diagnosis reagent.

The invention provides a viscosity response type fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of biochemical detection and analysis. The chemical structural formula of the fluorescent probe is shown in the specification, X represents halogen, and n is an integer between 0 and 10. The method can be applied to specific viscosity detection, targeted positioning of cell membranes and distinguishing of normal and tumor cells by utilizing the viscosity difference of the cell membranes. The probe synthesized by the invention can realize accurate detection of the viscosity change of the cell membrane.

The invention relates to the technical field of biochemical detection, in particular to a moving track control method of an automatic culture cabinet material conveying device. The moving track control method of the material conveying device comprises the following steps that after a telescopic mechanism is rotated and moved to a target storage layer of a target column storage rack, an inner ring rotating mechanism and an outer ring rotating mechanism are locked through a control module, and a telescopic rod enables an objective table to move to a first position from an initial state; and after the objective table reaches the first position, the objective table is lifted to a second position of the target storage layer through the lifting mechanism, and when the objective table carries a first material, after the vertical sensor sends a signal that the material conveying device obtains the first material, the objective table retracts to an initial state.

The invention relates to the technical field of biochemical detection, in particular to a pretreatment method of a propofol-containing sample, a propofol detection method and a kit. The pretreatment method of the propofol-containing sample comprises the following steps: providing the propofol-containing sample; the method comprises the following steps: adding porous polymer lipophilic magnetic beads into a sample, carrying out adsorption treatment, and then carrying out leaching treatment by using water and a methanol solution in sequence to obtain a propofol-magnetic bead compound; and eluting the propofol-magnetic bead compound by using an ammonia water methanol solution to obtain a sample to be detected. The pretreatment method provided by the invention is simple in process and low in cost, the to-be-detected sample containing propofol can be efficiently obtained, and the to-be-detected sample containing propofol can be subsequently used for high-sensitivity detection of propofol by an ion mobility spectrometry technology, so that the pretreatment method has a very good application prospect in the field of propofol detection.

The invention relates to a method and device for measuring and calculating the physiological age of a human endocrine system, electronic equipment and a storage medium. The method comprises the following steps: acquiring endocrine system indexes of a to-be-detected object, wherein the endocrine system indexes comprise a blood routine detection index, a blood biochemical detection index and a human whole body system scanner detection index of the to-be-detected object; acquiring a physiological age prediction model; and processing the endocrine system indexes by using the physiological age prediction model to obtain a target physiological age of the tested object. A physiological age analysis model is established based on blood endocrine related indexes and human whole body system scanner (eagle model) detection indexes, the physiological age of the human endocrine system is analyzed, and the aging degree of the body endocrine system is evaluated.

This utility model relates to the field of biochemical detection technology, and discloses a mechanism module and a modular biochemical analyzer. The mechanism module includes a reaction disk disposed in the center of the mechanism base, with several reaction cups arranged in a ring inside the reaction disk; a reaction disk drive module, the output end of which is connected to the reaction disk; a photoelectric colorimetric module disposed on the outside of the reaction disk; a sample dispensing mechanism for sample dispensing and cleaning; a reagent dispensing mechanism; a mixing mechanism; a cleaning mechanism for cleaning the reaction cups by dispensing and draining liquids; and a liquid path module. The sample dispensing mechanism, reagent dispensing mechanism, mixing mechanism, and cleaning mechanism are arranged sequentially along the rotation direction of the reaction disk. The mechanism base also includes an emergency tube holder, into which an emergency test tube rack is inserted, with several test tube-compatible insertion holes arranged in a ring on the emergency test tube rack.

The invention relates to a sample analyzer, and relates to the technical field of biochemical analysis. The sample analyzer provided by the invention comprises an optical module for performing optical detection on a sample reagent mixed solution in the cuvette. The optical module comprises a light source unit, the light source unit comprises a light source, the wavelength of light emitted by the light source can cover the wavelength required by biochemical detection, the wavelength of light emitted by the light source can cover the wavelength required by biochemical detection, and the light source comprises a plurality of light-emitting elements. The light emitted by the plurality of light-emitting elements jointly forms the light of each wavelength required by the detection unit; the light emitted by the plurality of light-emitting elements has the same optical path distance when reaching an object to be measured after being flattened, combined and expanded.