52 results about "Quantitative PCR instrument" patented technology

The invention relates to the technical field of microfluidics, in particular to a microfluidic isothermal amplification method and system based on multichannel spectrum detection, and the method comprises the following steps: adding a sample and a reaction premixed solution into a microfluidic chip to form a reaction liquid; at least two fluorescent probes are added into the reaction premixed solution; carrying out isothermal amplification reaction on the reaction liquid, and collecting spectrum time sequence data in the reaction process; and performing spectral deconvolution on the spectral time sequence data to respectively obtain an independent amplification curve corresponding to each fluorescent probe, and performing quantitative analysis. Aiming at the problem that a reaction amplification quantitative system in the prior art depends on a high-precision quantitative PCR instrument and an optical assembly and is relatively high in cost, a spectral deconvolution mode is introduced to separate independent amplification curves of all fluorescent probes on a calculation level, so that an expensive PCR instrument is omitted, and the cost is reduced. And the requirements on color filtering and detection precision of the optical assembly are reduced, and the method can be applied to detection scenes with relatively wide wave bands.

The invention belongs to the technical field of biology, and particularly relates to a primer probe combination and a kit for identifying a mycobacterium tuberculosis and mycobacterium avium complex. The composition for detecting mycobacterium tuberculosis comprises: an upstream primer having a nucleotide sequence as shown in SEQ NO: 1 or SEQ NO: 2; the nucleotide sequence of the downstream primer is as shown in SEQ NO: 3 or SEQ NO: 4 or SEQ NO: 5; a probe having a nucleotide sequence as shown in SEQ NO: 6; the composition for detecting the Mycobacterium avium complex comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is shown as SEQ NO: 7 or SEQ NO: 8 or SEQ NO: 9; the nucleotide sequence of the downstream primer is as shown in SEQ NO: 10 or SEQ NO: 11 or SEQ NO: 12; the nucleotide sequence of the probe is as shown in SEQ NO: 13. A new primer probe combination is screened out and used for differential diagnosis of Mtb and MAC, the primer probe combination has good specificity, the result is analyzed by a real-time fluorescent quantitative PCR instrument, the result is visual and continuous, operation is easy and rapid, and the false positive rate is low.

The invention discloses a method for detecting the state of a microsatellite site BAT-26, and belongs to the field of biological medicine, the sequences of a primer and a probe are shown in SEQ ID No: 1 to SEQ ID No: 11, the primer and the probe are adopted for detecting the state of the microsatellite site BAT-26 through a quantitative PCR end point fluorescence signal ratio method, the method has the advantages of being easy and rapid to operate and objective in result interpretation, uncovering electrophoresis detection is not needed, the detection time is shortened, and the detection efficiency is improved. Meanwhile, an expensive sequencer does not need to be purchased, and analysis can be carried out only by one fluorescent quantitative PCR instrument, so that the method is convenient to popularize.

The utility model discloses a PCR (Polymerase Chain Reaction) tube and a fluorescent quantitative PCR instrument. The PCR tube comprises a dustproof plug, a temperature control tube and a tube body for containing a detection reagent, a first opening is formed in the upper end of the tube body; the dustproof plug is arranged on the periphery of the temperature control pipe in a surrounding mode, and the dustproof plug and the temperature control pipe are arranged in the first opening and contained in the pipe body. The temperature control pipe is provided with a liquid conveying pipeline and a liquid discharging pipeline; the liquid conveying pipeline is used for introducing a heat-conducting medium, and the liquid discharging pipe is used for discharging the heat-conducting medium, so that the temperature control pipe can heat or cool a detection reagent in the pipe body. According to the technical scheme, the reaction time of the detection reagent is shortened.

The embodiment of the utility model provides a fluorescent quantitative PCR (Polymerase Chain Reaction) instrument and relates to the technical field of fluorescent detection equipment. The fluorescent quantitative PCR instrument comprises an instrument shell, a fluorescence detection subsystem and a metal bath subsystem, the fluorescence detection subsystem comprises a light source, a light filtering module, a rotary driving device, a reflection module and an imaging module, and the imaging module, the light filtering module and the imaging module are sequentially arranged; the filtering module is provided with a plurality of annularly arranged filtering channels, and the rotary driving device is connected with the filtering module; the metal bath subsystem comprises a heating module and a linear driving device, the rotary driving device is used for driving the light filtering module to rotate so as to switch different light filtering channels and transmit visible light emitted by the light source to the reflection module, and the reflection module is used for reflecting the visible light to a PCR plate placed on the heating module; and the fluorescent light generated in the holes of the PCR plate is reflected to the light filtering channel and is transmitted to the imaging module. The fluorescent quantitative PCR instrument is small in size and high in space utilization rate.

1. Name of the product in this design: Real-time PCR instrument. 2. Purpose of this design: This design is used for qualitative or quantitative detection of target nucleic acids in human samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention belongs to the technical field of genetic engineering and diagnostic reagents, and particularly relates to a brucella recombinase polymerase amplification (RPA) detection kit and application, and the detection kit comprises a specific primer group and a probe. According to the invention, the RPA amplification product is detected by using the test strip, after amplification is carried out for about 10 minutes, the RPA amplification product is dropwise added into the test strip to read a result, and a PCR (Polymerase Chain Reaction) amplification instrument and a quantitative PCR instrument are not needed. The obtained method has the advantages of strong specificity, good sensitivity, simple operation process, rapid detection and the like, provides a possibility for the detection of brucellosis in the field, and also provides a method for the research of brucellosis which does not aim at the diagnosis and treatment of diseases.

The utility model discloses an intestinal flora detection instrument which comprises a fluorescent quantitative PCR instrument, a bearing plate is arranged in the fluorescent quantitative PCR instrument in a sliding mode, a baffle plate cover is fixedly connected to the front face of the bearing plate, a bearing seat is arranged at the top of the bearing plate and used for bearing and storing an intestinal flora bearing container, and an installation assembly is arranged at the top of the bearing plate and used for installing the intestinal flora bearing container. The mounting assembly is used for clamping and locking the bearing seat, the mounting shell is arranged on the side of the bearing seat, the locking unit is arranged on the bearing plate, and the locking unit is used for clamping and locking the mounting shell and the bearing seat. The fluorescent quantitative PCR instrument, the bearing plate, the bearing seat and the mounting assembly are used in a matched mode, when the bearing seat and the mounting shell are located on the bearing plate, the locking unit can lock the bearing seat and the mounting shell, and the mounting shell slides away from the bearing seat, so that the mounting shell drives the locking assembly to unlock, and the locking effect is good. Therefore, the bearing seat can be directly detached, cleaned and disinfected.

The invention discloses a multiple PCR screening method for CYP2C19 genotyping. The multiple PCR screening method comprises the following steps: DNA extraction: extracting a target DNA sample from blood through a lysis method; pCR amplification: mixing the DNA sample, a primer, nuclease, a reaction buffer solution, dNTPs, a fluorescent dye and a fluorescence labeling probe, carrying out PCR amplification, and carrying out electrophoresis detection; s2, putting the PCR reaction solution detected in S2 into a reaction tube, then putting the reaction tube into a fluorescent quantitative PCR instrument, and collecting a fluorescent signal at 75 DEG C; and judging a result. According to the scheme, the detection method is adopted for detection, the obtained detection result is high in stability, and the drug agency conditions corresponding to CYP2C19 genotypes in different human bodies can be rapidly and accurately judged, so that subsequent treatment is facilitated. Through setting of PCR amplification parameters in the scheme, the detection accuracy can be improved, meanwhile, an amplification result test is carried out after amplification, it is guaranteed that all products entering a tester are successfully amplified, and the experiment efficiency and accuracy are improved.

The present invention discloses a real-time fluorescence quantitative PCR instrument, comprising a housing, a thermal cycle module, a detection module, and a sample assembly, wherein the thermal cycle module is used to provide the real-time temperature required by the sample assembly during the detection process; the detection module is used to perform real-time detection on the sample assembly; an entrance and an exit are provided on the housing, and the sample assembly enters and exits the housing through the entrance and an exit; the sample assembly comprises a rotating disk and a reagent kit, wherein the reagent kit is placed on the rotating disk and is detachably connected to the rotating disk, the rotating disk is rotatably connected to the housing, and the reagent kit is used to place a reagent tube to be detected. The present invention can effectively improve the efficiency of thermal cycles and ensure that subsequent operations can be seamlessly connected, thereby improving the detection efficiency as a whole. At the same time, the detection structure of the present invention can make the product design more miniaturized.

The utility model discloses a PCR module movable fluorescent quantitative PCR instrument which comprises a fluorescent quantitative PCR instrument body, a square groove is formed in one side of the front face of the fluorescent quantitative PCR instrument body, an ejection assembly is arranged in the square groove, a detection table is arranged at the top of the ejection assembly, and a fluorescent quantitative PCR module is arranged on the detection table. The two sides of the outer surface of the fluorescent quantitative PCR instrument body are fixedly connected with connecting pieces, and positioning assemblies used for improving stability penetrate through the middle threads of the top faces of the connecting pieces. According to the utility model, when the ejection assembly, the detection table and the clamping spring are used in cooperation, after a person detects a detected object, the person can press the clamping block to contract the clamping block into the square groove, and then the rolling ball slides along the inner wall of the square groove, so that the ejection spring is separated from the constraint; and then, the ejection spring can drive the placement plate to be automatically pushed out of the interior of the square groove, so that the personnel can conveniently and quickly take the detected object.

The utility model discloses a real-time fluorescent convection PCR (Polymerase Chain Reaction) instrument, relates to the technical field of molecular diagnosis, and solves the technical problem of poor heat dissipation effect of the existing fluorescent quantitative PCR instrument. The device comprises a first host shell, a first upper-layer support and a first lower-layer support are installed in the first host shell, a fluorescence excitation module and a fluorescence detection module are installed on one side of the first upper-layer support, and a mainboard is arranged on the other side of the first upper-layer support. The main board is connected with the first upper-layer support through a multi-layer heat dissipation assembly, a circuit board and a power module are installed on the first lower-layer support, a second host shell is installed on one side of the first host shell, and a convection heating module used for containing a convection PCR reaction tube is installed in the second host shell. According to the utility model, the problems that the circuit module cannot work normally and errors are increased due to overheating can be prevented.

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) instrument which comprises a fluorescent quantitative PCR instrument body, an upper heating cover, a front end plate and a rear tail cover, the upper heating cover is mounted at an opening in the top end of the fluorescent quantitative PCR instrument body in a front-back sliding manner in the Y-axis direction, and the front end plate and the rear tail cover are fixed to the front end and the rear end of the upper heating cover. And a T-shaped electric heating plate is mounted in the multi-rod type auxiliary heater. According to the invention, the stability of a reaction system and the reliability of optical detection are remarkably improved by adopting the multi-rod auxiliary heater and the T-shaped electric heating plate and combining the structural design of the flow-concentrating turbine active gas pumping assembly, the laminated flow-dividing gas pipe group and the drying filter and by virtue of a precise temperature control and dynamic water-gas management mechanism.

1. The name of the design product: real-time fluorescent quantitative PCR instrument. 2. The use of the design product: for real-time fluorescent quantitative PCR detection. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses a multi-module fluorescent quantitative PCR instrument, and relates to the technical field of nucleic acid detection equipment; the following scheme is provided, which comprises a plurality of independent thermal cycle modules, each of which is provided with an independent uniform temperature plate and a heating and heat dissipation mechanism and is used for independently running a PCR program; further comprising a multi-module heat insulation seat, the multi-module heat insulation seat is provided with a plurality of independent cavities corresponding to the plurality of thermal cycle modules, and a heat insulation structure is arranged between adjacent cavities; a partition plate structure for separating the cavity into a heat management cavity and an exhaust management cavity is arranged in each independent cavity, and a ventilation opening is arranged between the heat management cavity and the exhaust management cavity; a movable air volume adjusting piece is arranged in the exhaust management cavity. Through the hollow region arranged between the adjacent cavities on the multi-module heat insulation seat, an effective physical heat insulation barrier is formed, heat conduction and thermal interference between adjacent thermal cycle modules are significantly reduced, and the accuracy and repeatability of PCR amplification results are improved.

The invention discloses a specific primer and a method for rapidly identifying Chinese herbal medicine semen allii tuberosi and semen allii fistulosi. The specific primer comprises the following developed specific primer pairs: JP1-F and JP1-R; according to the present invention, the specific sequences of the semen allii tuberosi and the semen allii fistulosi can be amplified by using the fluorescent quantitative PCR instrument, the nucleic acid dyeing electrophoresis is not required, the rapid identification can be achieved by detecting the fluorescence signal, the whole identification period only needs 3 h, and the method has characteristics of simple operation, strong specificity, no toxic reagent, no influence of the sample appearance, the production place and other factors, and can be used for the rapid identification of the semen allii tuberosi and the semen allii fistulosi. And the method has a relatively strong application prospect.

The invention discloses a KASP molecular marker linked with a tobacco black shank resistance Ph introduction fragment and application of the KASP molecular marker. The invention discloses a KASP molecular marker linked with a tobacco black shank resistance Ph introduction fragment. The sequences of a primer pair of the KASP molecular marker are as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses application of the KASP molecular marker linked with the tobacco black shank resistance Ph introduction fragment in screening of tobacco with black shank resistance or application in breeding of tobacco varieties with black shank resistance. According to the KASP molecular marker, the simplicity of conventional PCR and the high sensitivity of fluorescence detection are fused, the experiment cost is greatly reduced, and the detection flux is improved. According to the method disclosed by the invention, only a small-scale breeding laboratory is usually provided with necessary instruments and equipment, electrophoretic analysis is not needed any more, and sample detection can be completed on a microplate reader or a quantitative PCR instrument by utilizing a 96-pore plate or a 384-pore plate.

The invention discloses a Klebsiella pneumoniae typing detection primer group based on multiple fluorescent quantitative PCR (Polymerase Chain Reaction), a kit and a detection method. The primer group comprises a klebsiella pneumoniae 16S upstream primer and a klebsiella pneumoniae 16S downstream primer as shown in SEQ ID NO.1-SEQ ID NO.14 in a sequence table; upstream and downstream primers of klebsiella pneumoniae K1 type; an upstream primer and a downstream primer of klebsiella pneumoniae K2 type; an upstream primer and a downstream primer of the Klebsiella pneumoniae K5 type; an upstream primer and a downstream primer of the klebsiella pneumoniae K20 type; an upstream primer and a downstream primer of the klebsiella pneumoniae K54 type; the invention discloses upstream and downstream primers of klebsiella pneumoniae K57. The kit has good specificity and sensitivity, and the result is analyzed by a real-time fluorescent quantitative PCR instrument and is visual and continuous. The detection scheme has the advantages that the operation is simple and rapid, the false positive rate is low, the detection of a large batch of samples can be met, the detection time is shortened, and the detection convenience is realized.

The present invention discloses a microfluidic chip that can be adapted to a fluorescent quantitative PCR instrument, which belongs to the field of molecular diagnosis technology and is used to solve the problems of uneven sample loading and complicated procedures in the prior art when testing large quantities of samples. The microfluidic chip includes a chip body with a plurality of visualized reaction pools, the shape of the chip body is adapted to the PCR instrument, and at least one bent microfluidic channel is provided in the chip body, and the lower convex parts of the microfluidic channel are connected to the reaction pools. The capillary bending area at the upper convex part of the microfluidic can prevent the liquid in the microfluidic channel from flowing due to centrifugal acceleration during centrifugal separation, so that the sample loading liquid flowing into the reaction pool is more uniform, thereby solving the problem of uneven sample loading of the sample loading liquid and improving the use effect.

The present invention provides a primer probe set for detecting yellowish Neisseria, a kit and an application thereof, and belongs to the field of gene detection technology. The present invention designs a pair of yellowish Neisseria-specific amplification products and a probe, which can quickly and accurately identify saliva stains. The primer probe set for detecting yellowish Neisseria provided by the present invention has high specificity for saliva stain samples, and the accuracy rate in body fluid identification can reach 100%. The primer probe set provided by the present invention has high detection sensitivity, and can be detected in samples as low as 0.625 copies / μL. The kit for detecting yellowish Neisseria provided by the present invention can detect in samples stored for 10 days, with a detection rate of 100%, and can partially detect in samples stored for more than 3 years, with a detection rate of 20%, which is of great significance in actual forensic identification work. The detection process does not require an amplifier, an electrophoresis instrument and a quantitative PCR instrument, and can be used for rapid testing at the forensic site.

The application provides a method for improving the detection rate of norovirus in aquatic products, which comprises the following steps: first, using a proteinase K solution to enzymolysis the digestive gland of shellfish, then adding a TGBE solution to elute, then adding enzymolysis corn starch to block activated carbon to remove the RT-qPCR inhibitor in the detection sample, taking the supernatant to extract nucleic acid, and using a fluorescent quantitative PCR instrument to detect. The detection method of the application reduces the inhibitor in the shellfish sample which interferes with the fluorescent quantitative PCR by using enzymolysis corn starch. In combination with the proteinase K enzymolysis, the TGBE elution and the RT-qRCR, the norovirus in the shellfish sample is detected, and the detection rate of the shellfish sample is increased by 10-20 times in 4h compared with the national standard method. The virus recovery rate and the detection sensitivity are greatly improved, and the method is suitable for shellfish samples, and has the characteristics of simple operation, high detection sensitivity and short time consumption.

1. The name of the design product: real-time fluorescent quantitative PCR instrument. 2. The use of the design product: for real-time fluorescent quantitative polymerase chain reaction instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a bottled beverage detection method, device and equipment and a medium, and relates to the technical field of artificial intelligence. Comprising the steps of obtaining metal impurity data and weight data of a beverage sample based on a preset sensor; scanning the beverage sample based on a preset spectrum technology to obtain corresponding spectrum data, and determining component data of the beverage sample based on the spectrum data by using a preset artificial intelligence algorithm; the method comprises the following steps: amplifying and detecting a target microorganism gene segment in a beverage sample by using a fluorescent quantitative PCR instrument to determine whether a target bacterium exists in the beverage sample, and performing specific binding with the target bacterium by using a preset biological recognition element to obtain bacterium detection data of the beverage sample; and analyzing and processing the metal impurity data, the weight data, the component data and the fungus detection data by using a preset artificial intelligence algorithm to obtain a detection result of the beverage sample. Therefore, appearance defects, microbial contamination or foreign matter doping of bottled beverages can be quickly identified.

The utility model discloses a portable small fluorescent quantitative PCR (polymerase chain reaction) instrument, which belongs to the field of medical instruments and comprises a cover body, a shell, a heating module and an amplification detection module, the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigeration sheet, a cooling sheet and a fan, and the amplification sample block is arranged in the shell. The amplification sample block is used for placing a PCR test tube, the amplification sample block and the cooling fin are detachably connected with the fan, and the refrigeration sheet is clamped between the amplification sample block and the cooling fin. The device is free of moving parts inside, small in size, light in weight, good in damping effect and convenient to carry; the requirement for a user is low, operation is easy, automatic detection is achieved when a power switch is turned on, additional operation is not needed, and the requirements of professionals and family use can be met at the same time; the heat dissipation effect is good, the heating sample block is small in size, the temperature circulation speed is high, the detection time is shortened, and rapid detection is facilitated.

The utility model discloses a sample adding device for a fluorescent quantitative PCR instrument, which comprises a sample adding table, an equipment frame is fixedly connected to the center of the top of the sample adding table, belt pulleys are arranged at the two ends of the top of the sample adding table, the outer walls of the two belt pulleys are jointly connected with a conveying belt through a belt, and the outer side wall of the conveying belt is fixedly connected with a storage mechanism. A driving motor corresponding to the belt pulley is fixedly connected to one end of the top of the equipment frame, and a transverse frame is fixedly connected to the center of the top of the equipment frame. According to the quantitative sample adding device, the object placing mechanism and the material adding mechanism are matched with each other, a sample to be added is pumped into the quantitative cylinder through the piston, the sample is directly pushed into a PCR tube during sample adding, the total quantity of pre-loaded samples in the quantitative cylinder is visual and transparent in the process, and even if multiple groups of different quantities of samples are added at the same time, the samples are added through the outside of the quantitative cylinder. A worker still can visually monitor the sample adding condition, so that the worker can better control the sample adding process of the PCR tube.

The invention provides a detection method of XPV / POLH single base mutation and application of the detection method. The detection method comprises the following steps: by taking nucleic acid of a sample to be detected as template DNA, carrying out real-time fluorescence collection through PCR reaction, drawing an amplification curve and a melting curve, and carrying out result interpretation; the interpretation of the result comprises interpretation of mutation sites of four missense mutations and three nonsense mutations of the POLH gene. On the basis of a nucleic acid extraction method, detection of missense mutation and nonsense mutation of the POLH gene by using a melting curve is established, and the method has the advantages that operation is simple, operation can be carried out on the basis of PCR, consumed time is short, the whole process only needs about 2-3 hours, special equipment is not needed, and the method is suitable for large-scale popularization and application. A conventional fluorescent quantitative PCR instrument can realize interpretation of a detection result, the detection method is simple, the cost is low, the real-time fluorescent quantitative PCR method is equivalent, and a reliable and accurate basis can be quickly provided for auxiliary diagnosis of XP diseases.

The invention relates to the technical field of detection instruments, in particular to a fluorescent quantitative PCR (polymerase chain reaction) instrument based on a sample multichannel detection device, which comprises a thermal cycle module and an optical detection system, the optical detection system consists of a plurality of independent detection units, each unit corresponds to a single reaction hole of the thermal cycle module, and the thermal cycle module is connected with the optical detection system. The device comprises an independent light source, a filtering assembly and a detector. A positioning frame is arranged between the thermal cycle module and the optical detection system, and the optical axis of each detection unit is forcibly aligned with the central axis of the corresponding reaction hole by the frame; a thermal expansion compensation mechanism is integrated at the bottom of the thermal cycle module and counteracts module flatness deviation caused by temperature change through reverse deformation. The method has the detection efficiency effects of high stability, high precision and high repeatability.

The present application relates to the technical field of microfluidics, and particularly relates to a microfluidic isothermal amplification method and system based on multi-channel spectral detection, comprising: adding a sample and a reaction premix to a microfluidic chip to form a reaction liquid; at least two fluorescent probes are added to the reaction premix; performing isothermal amplification reaction on the reaction liquid, collecting spectral time series data during the reaction process; performing spectral deconvolution on the spectral time series data to obtain independent amplification curves corresponding to each fluorescent probe and perform quantitative analysis. In view of the problem that the reaction amplification quantitative system in the prior art relies on high-precision quantitative PCR instruments and optical components, and the cost is high, the spectral deconvolution method is introduced to separate the independent amplification curves of each fluorescent probe at the calculation level, thereby eliminating the expensive PCR instrument, and reducing the requirement for the color filter and detection accuracy of the optical component, and the method can be suitable for a wide range of detection scenarios.

1. The name of the product of this design: Fluorescence quantitative PCR instrument. 2. Purpose of the product of this appearance design: to conduct fluorescent quantitative PCR detection on the test samples. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

1. The name of the design product: fluorescent quantitative PCR instrument (YZ9048). 2. The use of the design product: for detecting samples by fluorescent quantitative PCR. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.