19 results about "Quantitative PCR instrument" patented technology

1. Name of the product in this design: Real-time PCR instrument. 2. Purpose of this design: This design is used for qualitative or quantitative detection of target nucleic acids in human samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention belongs to the technical field of genetic engineering and diagnostic reagents, and particularly relates to a brucella recombinase polymerase amplification (RPA) detection kit and application, and the detection kit comprises a specific primer group and a probe. According to the invention, the RPA amplification product is detected by using the test strip, after amplification is carried out for about 10 minutes, the RPA amplification product is dropwise added into the test strip to read a result, and a PCR (Polymerase Chain Reaction) amplification instrument and a quantitative PCR instrument are not needed. The obtained method has the advantages of strong specificity, good sensitivity, simple operation process, rapid detection and the like, provides a possibility for the detection of brucellosis in the field, and also provides a method for the research of brucellosis which does not aim at the diagnosis and treatment of diseases.

The utility model discloses an intestinal flora detection instrument which comprises a fluorescent quantitative PCR instrument, a bearing plate is arranged in the fluorescent quantitative PCR instrument in a sliding mode, a baffle plate cover is fixedly connected to the front face of the bearing plate, a bearing seat is arranged at the top of the bearing plate and used for bearing and storing an intestinal flora bearing container, and an installation assembly is arranged at the top of the bearing plate and used for installing the intestinal flora bearing container. The mounting assembly is used for clamping and locking the bearing seat, the mounting shell is arranged on the side of the bearing seat, the locking unit is arranged on the bearing plate, and the locking unit is used for clamping and locking the mounting shell and the bearing seat. The fluorescent quantitative PCR instrument, the bearing plate, the bearing seat and the mounting assembly are used in a matched mode, when the bearing seat and the mounting shell are located on the bearing plate, the locking unit can lock the bearing seat and the mounting shell, and the mounting shell slides away from the bearing seat, so that the mounting shell drives the locking assembly to unlock, and the locking effect is good. Therefore, the bearing seat can be directly detached, cleaned and disinfected.

The invention discloses a multiple PCR screening method for CYP2C19 genotyping. The multiple PCR screening method comprises the following steps: DNA extraction: extracting a target DNA sample from blood through a lysis method; pCR amplification: mixing the DNA sample, a primer, nuclease, a reaction buffer solution, dNTPs, a fluorescent dye and a fluorescence labeling probe, carrying out PCR amplification, and carrying out electrophoresis detection; s2, putting the PCR reaction solution detected in S2 into a reaction tube, then putting the reaction tube into a fluorescent quantitative PCR instrument, and collecting a fluorescent signal at 75 DEG C; and judging a result. According to the scheme, the detection method is adopted for detection, the obtained detection result is high in stability, and the drug agency conditions corresponding to CYP2C19 genotypes in different human bodies can be rapidly and accurately judged, so that subsequent treatment is facilitated. Through setting of PCR amplification parameters in the scheme, the detection accuracy can be improved, meanwhile, an amplification result test is carried out after amplification, it is guaranteed that all products entering a tester are successfully amplified, and the experiment efficiency and accuracy are improved.

The utility model discloses a PCR module movable fluorescent quantitative PCR instrument which comprises a fluorescent quantitative PCR instrument body, a square groove is formed in one side of the front face of the fluorescent quantitative PCR instrument body, an ejection assembly is arranged in the square groove, a detection table is arranged at the top of the ejection assembly, and a fluorescent quantitative PCR module is arranged on the detection table. The two sides of the outer surface of the fluorescent quantitative PCR instrument body are fixedly connected with connecting pieces, and positioning assemblies used for improving stability penetrate through the middle threads of the top faces of the connecting pieces. According to the utility model, when the ejection assembly, the detection table and the clamping spring are used in cooperation, after a person detects a detected object, the person can press the clamping block to contract the clamping block into the square groove, and then the rolling ball slides along the inner wall of the square groove, so that the ejection spring is separated from the constraint; and then, the ejection spring can drive the placement plate to be automatically pushed out of the interior of the square groove, so that the personnel can conveniently and quickly take the detected object.

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) instrument which comprises a fluorescent quantitative PCR instrument body, an upper heating cover, a front end plate and a rear tail cover, the upper heating cover is mounted at an opening in the top end of the fluorescent quantitative PCR instrument body in a front-back sliding manner in the Y-axis direction, and the front end plate and the rear tail cover are fixed to the front end and the rear end of the upper heating cover. And a T-shaped electric heating plate is mounted in the multi-rod type auxiliary heater. According to the invention, the stability of a reaction system and the reliability of optical detection are remarkably improved by adopting the multi-rod auxiliary heater and the T-shaped electric heating plate and combining the structural design of the flow-concentrating turbine active gas pumping assembly, the laminated flow-dividing gas pipe group and the drying filter and by virtue of a precise temperature control and dynamic water-gas management mechanism.

The application discloses a multi-module fluorescent quantitative PCR instrument, and relates to the technical field of nucleic acid detection equipment; the following scheme is provided, which comprises a plurality of independent thermal cycle modules, each of which is provided with an independent uniform temperature plate and a heating and heat dissipation mechanism and is used for independently running a PCR program; further comprising a multi-module heat insulation seat, the multi-module heat insulation seat is provided with a plurality of independent cavities corresponding to the plurality of thermal cycle modules, and a heat insulation structure is arranged between adjacent cavities; a partition plate structure for separating the cavity into a heat management cavity and an exhaust management cavity is arranged in each independent cavity, and a ventilation opening is arranged between the heat management cavity and the exhaust management cavity; a movable air volume adjusting piece is arranged in the exhaust management cavity. Through the hollow region arranged between the adjacent cavities on the multi-module heat insulation seat, an effective physical heat insulation barrier is formed, heat conduction and thermal interference between adjacent thermal cycle modules are significantly reduced, and the accuracy and repeatability of PCR amplification results are improved.

The application provides a method for improving the detection rate of norovirus in aquatic products, which comprises the following steps: first, using a proteinase K solution to enzymolysis the digestive gland of shellfish, then adding a TGBE solution to elute, then adding enzymolysis corn starch to block activated carbon to remove the RT-qPCR inhibitor in the detection sample, taking the supernatant to extract nucleic acid, and using a fluorescent quantitative PCR instrument to detect. The detection method of the application reduces the inhibitor in the shellfish sample which interferes with the fluorescent quantitative PCR by using enzymolysis corn starch. In combination with the proteinase K enzymolysis, the TGBE elution and the RT-qRCR, the norovirus in the shellfish sample is detected, and the detection rate of the shellfish sample is increased by 10-20 times in 4h compared with the national standard method. The virus recovery rate and the detection sensitivity are greatly improved, and the method is suitable for shellfish samples, and has the characteristics of simple operation, high detection sensitivity and short time consumption.

1. The name of the design product: real-time fluorescent quantitative PCR instrument. 2. The use of the design product: for real-time fluorescent quantitative polymerase chain reaction instrument. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a bottled beverage detection method, device and equipment and a medium, and relates to the technical field of artificial intelligence. Comprising the steps of obtaining metal impurity data and weight data of a beverage sample based on a preset sensor; scanning the beverage sample based on a preset spectrum technology to obtain corresponding spectrum data, and determining component data of the beverage sample based on the spectrum data by using a preset artificial intelligence algorithm; the method comprises the following steps: amplifying and detecting a target microorganism gene segment in a beverage sample by using a fluorescent quantitative PCR instrument to determine whether a target bacterium exists in the beverage sample, and performing specific binding with the target bacterium by using a preset biological recognition element to obtain bacterium detection data of the beverage sample; and analyzing and processing the metal impurity data, the weight data, the component data and the fungus detection data by using a preset artificial intelligence algorithm to obtain a detection result of the beverage sample. Therefore, appearance defects, microbial contamination or foreign matter doping of bottled beverages can be quickly identified.

The invention relates to the technical field of detection instruments, in particular to a fluorescent quantitative PCR (polymerase chain reaction) instrument based on a sample multichannel detection device, which comprises a thermal cycle module and an optical detection system, the optical detection system consists of a plurality of independent detection units, each unit corresponds to a single reaction hole of the thermal cycle module, and the thermal cycle module is connected with the optical detection system. The device comprises an independent light source, a filtering assembly and a detector. A positioning frame is arranged between the thermal cycle module and the optical detection system, and the optical axis of each detection unit is forcibly aligned with the central axis of the corresponding reaction hole by the frame; a thermal expansion compensation mechanism is integrated at the bottom of the thermal cycle module and counteracts module flatness deviation caused by temperature change through reverse deformation. The method has the detection efficiency effects of high stability, high precision and high repeatability.

The present application relates to the technical field of microfluidics, and particularly relates to a microfluidic isothermal amplification method and system based on multi-channel spectral detection, comprising: adding a sample and a reaction premix to a microfluidic chip to form a reaction liquid; at least two fluorescent probes are added to the reaction premix; performing isothermal amplification reaction on the reaction liquid, collecting spectral time series data during the reaction process; performing spectral deconvolution on the spectral time series data to obtain independent amplification curves corresponding to each fluorescent probe and perform quantitative analysis. In view of the problem that the reaction amplification quantitative system in the prior art relies on high-precision quantitative PCR instruments and optical components, and the cost is high, the spectral deconvolution method is introduced to separate the independent amplification curves of each fluorescent probe at the calculation level, thereby eliminating the expensive PCR instrument, and reducing the requirement for the color filter and detection accuracy of the optical component, and the method can be suitable for a wide range of detection scenarios.

1. The name of the design product: fluorescent quantitative PCR instrument (YZ9048). 2. The use of the design product: for detecting samples by fluorescent quantitative PCR. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

The invention discloses a full-automatic sample adding fluorescent quantitative PCR instrument based on biological testing, and relates to the technical field of biomedical engineering.The full-automatic sample adding fluorescent quantitative PCR instrument comprises an analysis mechanism, a processing mechanism is arranged in a shell and comprises a connecting block, and two processing tanks are arranged on the upper side of the connecting block; a flow guide shell and a connecting shell are additionally arranged on the upper side of the connecting block, a fan is additionally arranged between the interior of the flow guide shell and the interior of the connecting shell, the remaining two air inlet ends of a communicating pipe communicate with first electric valves, and two air outlets in the top of the other end of the connecting block communicate with second electric valves; by arranging the treatment mechanism, key areas in the instrument can be comprehensively covered and disinfected, meanwhile, disinfection gas left in the instrument can be treated, the disinfection effect is good, and the disinfection effect is good. Therefore, the use efficiency of the full-automatic sample adding fluorescent quantitative PCR instrument based on the biological test is improved.

1. The name of the design product: fluorescent quantitative PCR instrument. 2. The use of the design product: the design product is suitable for qualitative or quantitative detection of target nucleic acid in human samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

1. The name of the design product: real-time fluorescent quantitative PCR instrument (InCycle Mini). 2. The use of the design product: for clinical qualitative and quantitative detection of target nucleic acid (DNA / RNA) from the sample to be tested. 3. The design points of the design product: the combination of shape and color. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The design claimed contains color.

The invention discloses a method for detecting gelatin in sea cucumber based on a fluorescent PCR (Polymerase Chain Reaction) technology. The method comprises the following steps: determining a target and designing a primer and a probe; determining a target gene: selecting a mitochondrial gene aiming at the DNA which is easy to degrade after processing, and designing a primer and a probe for amplifying a short fragment; obtaining a specific sequence: obtaining target gene sequences of the target sea cucumber and common adulterated species from an NCBI gene database, and finding a unique specific sequence region of the sea cucumber through comparison; synthesizing a primer and a probe: designing and synthesizing the primer and the TaqMan probe on the basis of the specific region; preparing a reference substance; sample pretreatment and DNA extraction; preparing a fluorescent PCR reaction system and adding a sample; running a fluorescent PCR amplification program: placing the reaction tube in a real-time fluorescent quantitative PCR instrument; result analysis and interpretation: quality control inspection and sample result interpretation. According to the invention, a primer and a probe which are only complementarily combined with sea cucumber DNA are designed, and a fluorescence signal is generated in PCR amplification, so that specific detection can be realized.

The invention provides an optical crosstalk correction method of a fluorescent quantitative PCR instrument and application of the optical crosstalk correction method, and belongs to the field of biological detection instruments. Through combination of hardware modular design and an intelligent algorithm, synchronous acquisition and accurate correction of multi-channel fluorescence signals are realized, the problems of large detection error and large equipment size are solved, and the high-throughput and high-precision requirements of multi-pathogen nucleic acid detection are met.

The invention relates to the technical field of medical detection, and discloses a schistosoma japonicum katsurada rapid detection method based on enzyme-mediated double-index amplification, and the method comprises the following steps: designing 6 upstream DNA primers, 6 downstream DNA primers and 6 RNA probes for a schistosoma japonicum katsurada CNUS0000103598 gene segment; collecting a to-be-detected sample; constructing a 20 [mu] L reaction system, and reacting for 30 minutes at a constant temperature of 42 DEG C; and finally, detecting a fluorescence signal in the reaction process by using a fluorescence quantitative PCR (Polymerase Chain Reaction) instrument. According to the present invention, the technical effect that the detection sensitivity reaches 14 pg / [mu] L is achieved through the dual index amplification of the EmDEA technology, i.e., DNA amplification + RNA signal amplification, and is far higher than the traditional PCR and LAMP technology, the schistosoma japonicum katsurada nucleic acid in the early infection or low-load sample can be detected, and the false negative rate is effectively reduced.