46 results about "Porcine Transmissible Gastroenteritis" patented technology

The invention discloses a medicinal composition for treating infectious gastroenteritis of a piglet. The medicinal composition includes the following constituents: 50-70 parts of pericarpium granati, 50-70 parts of ciliatenerve knotweed roots, 50-70 parts of flos hibisci, 40-60 parts of pulsatilla chinensis, 40-60 parts of sargentodoxa cuneata, 40-60 parts of patrinia villosa juss, 40-60 parts of fried coix seeds, 30-50 parts of dendranthema indicum, 30-50 parts of acalypha australis, 20-40 parts of agrimonia pilosa, 3-7 parts of berberine powder, 3-7 parts of piglet special bacteria and 3-7 parts of vitamin B complex. Through organic combination, dialectical compatibility and mutual assistance of the constituents, the functions of the medicinal composition for clearing heat, drying dampness and curing diarrhea is strengthened, and the purpose of curing diarrhea of the piglet is achieved; the medicinal composition can effectively prevent the piglet from suffering infectious gastroenteritis, is safe and nontoxic with a definite curative effect, and can improve the immunity of animals and the breeding benefit. Clinical trials prove that the cure rate of the medicinal composition for piglet diarrhea is 93.15%, with an effective rate of 98.97%.

The invention discloses a preparation method of a recombinant antimicrobial peptide extracted from a small intestine of a pig and an application thereof. The preparation method comprises the following steps: mashing, homogenizing and repeatedly freezing and thawing the small intestine tissue of the pig with a meat grinder and a colloid mill, and preliminarily crushing cells; then performing the water boiling heat treatment, and extracting with acetic acid solution to obtain a large amount of antimicrobial peptides; centrifuging to enable the quantity of other proteins to be reduced greatly, filtering the supernate obtained through centrifuging by a tangential flow micro-filtration membrane and a tangential flow ultrafiltration membrane sequentially, adjusting the pH value, filtering the supernate by a tangential flow nanofiltration membrane, concentrating and dialyzing to desalt, and disinfecting after adjusting the osmotic pressure to obtain the recombinant antimicrobial peptide extracted from the small intestine of the pig. According to the invention, the application of the recombinant antimicrobial peptide adopts effects of the antimicrobial peptide on aspects of mucosal immunity, immunopotentiators, novel antibiotics and the like, and the mucosal immunity character of the antimicrobial peptide can play an important role in the prevention and control of transmissible gastroenteristis, epidemic diarrhea and mycoplasma gallisepticum; the antimicrobial peptide replaces traditional antibiotics, and is remarkable in effect.

The invention provides a triple antigen and antibody complex for preventing and controlling porcine viral diarrhea and a preparation method of the triple antigen and antibody complex. The triple antigen and antibody complex comprises an antigen and an antibody, wherein the antigen is a triple virus liquid obtained by mixing porcine transmissible gastroenteritis viruses, porcine epidemic diarrhea viruses and porcine rotavirus, and the antibody is a triple egg yolk antibody prepared on the basis of the antigen. The triple antigen and antibody complex for preventing and controlling porcine viral diarrhea is convenient and quick to use and capable of safely and effectively preventing and controlling the porcine viral diarrhea. The triple antigen and antibody complex can effectively reduce the body immunological stress response, increases the protection production speed, rapidly increases the body antibody titer and is prevented from the virus diffusion risk in production and use processes.

The invention provides a porcine epizootic diarrhea, transmissible gastroenteritis and porcine deltacoronavirus triple subunit vaccine, which consists of an antigen and a vaccine adjuvant, wherein theantigen includes porcine epizootic diarrhea virus S1 protein which is expressed by an X33-PEDV-S1 strain that preservation number of CGMCC No.14372, transmissible gastroenteritis virus S1 protein which is expressed by an X33-TGEV-S1 strain that preservation number is CGMCC No.14371 and porcine deltacoronavirus S1 protein which is expressed by an X33-PDCoV-S1 strain that preservation number is CGMCC No.14370. The porcine epizootic diarrhea, transmissible gastroenteritis and porcine deltacoronavirus triple subunit vaccine provided by the invention is high in safety and good in immunogenicity, and the triple vaccine, after being applied to immunization, can rapidly generate an antibody and can keep antibody titer at a relatively high level for a long time; the vaccine is long in preservationperiod and low in immunizing dose; the adopted adjuvant is easy to inject; and three major diarrhea-related viral diseases can be prevented and treated by conducting injection once, so that the survival rate of piglets is improved.

The invention provides a detection kit containing two anti porcine epidemic diarrhea virus monoclonal antibodies PEDV-McAB1 and PEDV-McAB2, two anti porcine transmissible gastroenteritis virus monoclonal antibodies and two anti porcine rotavirus monoclonal antibodies; the kit can be applied for simultaneous detection of porcine epidemic diarrhea viruses, porcine transmissible gastroenteritis viruses and / or porcine rotaviruses with non-diagnostic purpose, moreover, the detection sensitivity of the kit for simultaneous detection of two or three kinds of viruses is higher than that of single detection of one kind of virus, and false positive results are avoided.