296 results about "Astrovirus gastroenteritis" patented technology

The invention relates to a Chinese traditional medicine and a preparation method thereof, in particular to a pure Chinese traditional medicine preventing preparation which can effectively treat outdated, superficial, and atrophic gastritis, ulcer, edema, and acute and chronic gastroenteritis, hepatocholangeitis, and malignancy, and can improve the immunity of the patients, and a preparation method thereof. The herbal preparation is composed of more than 100 pure Chinese traditional medicines according to the proportioning by weight: dzho milk made calcite, zuota, jade, coral, pearl, tabasheer, saffron, clove, nutmeg, cardamom, Fructus Tsaoko, bezoar, Phytolacca acinosa, yellow corydalis, iris, Panax pseudoginseng, coriander, rutabaga, double-blossom groundsel, red sal ammoniac, purple sal ammoniac, Sikkimese rock astragalus root, stenophyllous ground touching plum, Tibetan arris vegetable, Himalayas red Jessamine, caltrop, Nangjuguique, veronica, etc. The preparation method includes six procedures of crushing, mixing, making pill, polishing, and packaging. The Chinese traditional medicine of the invention has notable curative effect, simple medicament form, and simple production process, and is worthy to be popularized and applied.

The invention provides a method for preparing triple vaccine for preventing porcine transmissible gastroenteritis, porcine epidemic diarrhea and porcine rotavirus. The method comprises the following steps: inoculating a host-cell line with a 90 percent grown monostratum against a porcine transmissible gastroenteritis virus, a porcine epidemic diarrhea virus and a porcine rotavirus respectively, and adding a cell maintenance media into the host-cell lines respectively to be cultured at 37 DEG C; after cytopathic effect reaches over 75 percent, collecting viruses to be stored at 20 DEG C below zero for standby; mixing the viruses according to 10 TCID50 in 1:1:1, and simultaneously adding Freund's complete adjuvant and immunopotentiator into the mixture to inactivate the mixture by formaldehyde at 37 DEG C for 24 hours; and adding an oil adjuvant into the mixture to prepare a vaccine of water-oil-water preparation. The method can be used for preparing the triple vaccine for preventing the porcine transmissible gastroenteritis, the porcine epidemic diarrhea and the porcine rotavirus so as to solve the problem that the diseases do not have an effective medicine to treat currently.

The present invention relates to phage isolates having a strong lytic activity against pathogenic Enterobacteriaceae such as Escherichia coli and/or Salmonella strains and their use in various human or pet food products for the treatment or prevention of bacterial diseases caused by pathogenic Enterobacteriaceae such as Escherichia coli, in particular for phage therapy of pediatric gastroenteritis, or Salmonella infection. It also relates to human or pet food products prepared thereof.

The invention discloses a pure traditional Chinese medicine recipe for curing various animal diarrhoeal diseases and a preparation method of the pure traditional Chinese medicine recipe. The pure traditional Chinese medicine recipe comprises the following ingredients: 1 g of white paeony root, 1 g of atractylis ovata, 1 g of Chinese pulsatilla root, 1 g of rhizoma atractylodis, 2 g of dried orange peel, 1 g of folium isatidis, 1 g of cortex phellodendri, 0.75 g of fructus forsythiae, 3 g of dandelion, 1.5 g of ash bark, 5 g of garden burnet, 1 g of pomegranate rind and 1 g of tuckahoe. The recipe has an effect on curing various animal diarrhoeal diseases such as piglet's yellow-white dysentery, epidemic diarrhea and transmissible gastroenteritis; the preparation method is simple and convenient to operate; and suitability for popularization and application is obtained.

The invention provides a monoclonal antibody specially bound to TGEV (transmissible gastroenteritis of swine virus) and a kit prepared from the monoclonal antibody. The TGEV can be determined rapidlyand accurately, and strains can be widely determined. Pharmaceutical composition prepared from the antibody can be used for broad-spectrum prevention and treatment of different TGEVs.

The invention discloses a transmissible gastroenteritis virus (H) attenuated vaccine strain and the application. The microorganism preservation number of the attenuated vaccine strain of the invention is CCTCC-V200609. The safety of attenuated strain TGEV of the invention is excellent, which has excellent immune protective rate. The attenuated strain of the invention can be applied in preparing diagnostic reagent for diagnosing transmissible gastroenteritis, and also can be applied in preparing single vaccine or mixed vaccine (active or inactivated vaccine) and the like.

The invention discloses a traditional Chinese medicine composition for treating the stomach diseases and aims at solving the problems that the stomach diseases generally comprise multiple types simultaneously, have the characteristics of diversity and complexity, the western medicine is mainly used for treating the symptoms, and is difficult to treat the stomach diseases thoroughly, but the existing Chinese patent medicine for treating the stomach diseases can not aim at the characteristics of diversity and complexity of the stomach diseases completely to cause no healing for long time and repeated attack of most patients. The traditional Chinese medicine composition comprises pericarpium citri reticulatae viride, pericarpium citri reticulatae, rhizoma cyperi, radix bupleuri, adenophora stricta, pinellia ternata, rhizoma atractylodis macrocephalae, pearl barley, fructus aurantii, mangnolia officinalis and fructus amomi and the like. The traditional Chinese medicine composition disclosed by the invention has the advantages that the effect for treating the stomach diseases is good, the treating effects for multiple stomach diseases (such as stomachache, gastric acid, gastrectasia, acute and chronic gastroenteritis, superficial gastritis, atrophic gastritis, erosive gastritis, gastroenteric ulcer and gastroenteric metaplasia) are good, the difficulties of no healing for long time and repeated attacks of the stomach diseases are thoroughly solved, and the traditional Chinese medicine composition has the characteristics of fast effect, good treating effect, thorough treatment and low possibility of relapse on clinical application and is popular with vast patients.

The invention relates to a process for preparing Chinese medicinal composition for the treatment of chronic enteritis, gastritis and bacillary dysentery, which is prepared from Chinese herbal materials including hairy euphorbia, Chinese knotweed and iron holly bark through steps of grinding, grilling, merging grilling liquid, concentrating into electuary, drying and granulating.

An immunoglobulin for preventing the infectious gastroenteritis, epidemic diarrhea and rotavirus of hog is prepared from their immunogens through cell culture, concentrating and proportionally mixing it with complete Frennd's adjuvant. It features that the glycoside-peptide injection is used as its immunopotentiator.

The invention discloses a healthcare snake feed capable of preventing snake gastroenteritis and a preparation method thereof. The healthcare snake feed capable of preventing snake gastroenteritis comprises the following raw materials in parts by weight: 15 to 17 parts of fish scale, 12 to 16 parts of shrimp head, 25 to 28 parts of earthworm, 12 to 16 parts of fish meat, 8 to 10 parts of chicken meat, 20 to 25 parts of soybean meal, 18 to 21 parts of needle mushroom root, 8 to 10 parts of corncob, 5 to 7 parts of rice husk, 25 to 30 parts of lotus root starch, 10 to 13 parts of fruit peel, 1 to 1.5 parts of tartaric acid, 3 to 5 parts of traditional Chinese medicine preparation, 0.8 to 1 part of additive, 0.002 to 0.003 part of bifidobacterium, 0.001 to 0.0015 part of lactobacillus, 0.003 to 0.0045 part of saccharomycetes and 0.002 to 0.003 part of bacillus aceticus. The healthcare snake feed capable of preventing snake gastroenteritis disclosed by the invention has the advantages that the snake feeding amount can be improved, the snake immunity is improved, the morbidity of snake gastroenteritis is reduced, thereby the snake skin disease is prevented and the production cost of the feed is reduced.

The invention provides a porcine epizootic diarrhea, transmissible gastroenteritis and porcine deltacoronavirus triple subunit vaccine, which consists of an antigen and a vaccine adjuvant, wherein theantigen includes porcine epizootic diarrhea virus S1 protein which is expressed by an X33-PEDV-S1 strain that preservation number of CGMCC No.14372, transmissible gastroenteritis virus S1 protein which is expressed by an X33-TGEV-S1 strain that preservation number is CGMCC No.14371 and porcine deltacoronavirus S1 protein which is expressed by an X33-PDCoV-S1 strain that preservation number is CGMCC No.14370. The porcine epizootic diarrhea, transmissible gastroenteritis and porcine deltacoronavirus triple subunit vaccine provided by the invention is high in safety and good in immunogenicity, and the triple vaccine, after being applied to immunization, can rapidly generate an antibody and can keep antibody titer at a relatively high level for a long time; the vaccine is long in preservationperiod and low in immunizing dose; the adopted adjuvant is easy to inject; and three major diarrhea-related viral diseases can be prevented and treated by conducting injection once, so that the survival rate of piglets is improved.

The invention belongs to the technical field of animal virology and animal infectious diseases and specifically relates to a primer and a multiple RT-PCR method for identifying and detecting swine diarrhea coronavirus. The swine diarrhea coronavirus relates to classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis. The invention discloses a multiple RT-PCR primer and a detecting method for simultaneously detecting the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swinetransmissible gastroenteritis; the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis which seriously impair pig industry can be simultaneously detected and distinguished through once experiment; a new, quick and effective detecting method is supplied for identifying the viruses and diagnosing the clinic diseases; the detection time can be shortened; and the detection cost can be lowered.

The invention relates to a detection primer and reagent kit of four RT-PCR of porcine epidemic diarrheaviruses, transmissible gastroenteritis of swine, porcine rotaviruses and porcine deltacoronaviruses, and belongs to the technical field of molecular detection. The fourfold RT-PCR detection primer comprises detection primers PEDV F and PEDV R of porcine epidemic diarrhea viruses, detection primers TGEV F and TGEV R of the transmissible gastroenteritis of swine, detection primers PoRV F and PoRV R of the porcine rotaviruses and detection primers PDCoV-F and PDCoV-R of the porcine delta coronaviruses. The primer is high in specificity, has repeatability, is high in sensitivity and is high in clinical reliability.

The invention belongs to the technical field of fermenting beverages, and discloses a dried orange peel fermenting health-care beverage with an intestines and stomach improving function and a preparation method thereof. The dried orange peel fermenting health-care beverage is prepared from the following components in parts by weight: 20 to 30 parts of dried orange peel, 1 to 2 parts of liquorice root, 5 to 10 parts of seed of wild jujube, 5 to 10 parts of herba menthae, 1 to 2 parts of poria cocos, 0.8 to 1.2 parts of fructus amomi, 2 to 4 parts of yam, 1 to 2 parts of pogostemon cablin, 15 to 20 parts of bergamot fruit, 15 to 20 parts of passion fruit, 2 to 5 parts of honey, and 60 to 80 parts of water. The dried orange peel fermenting health-care beverage has the advantages that the effective ingredients in raw materials are sufficiently released by enzymolysis technique, and the toxic and side effect in traditional Chinese medicine is decreased by fermenting technique; the obtained dried orange peel fermenting health-care beverage has fragrances of passion fruit and herba menthae, the function of releasing discomfortability of intestines and stomach is good, and the function of assisting treatment on gastroenteritis caused by pathogenic bacteria, such as helicobacter pylori, is realized; the effects of tranquilizing and allaying excitement, resisting aging and cancers, enhancing immunity and the like are realized.

The invention discloses a Taqman real-time fluorescent PCR kit for detecting pig umbilical cord blood transmissible gastroenteritis virus and application thereof. The kit comprises a pair of primers and a specific fluorescent probe, the sequences of the primers are shown as SEQ ID NO:1 and SEQ ID NO:2, and the sequence of the specific fluorescent probe are shown as SEQ ID NO:3. The Taqman real-time fluorescent PCR kit can specifically detect the pig umbilical cord blood transmissible gastroenteritis virus, and has high sensitivity, strong capacity of resisting disturbance, strong universality and good repeatability. The kit can precisely evaluate and diagnose conditions of carrying and expelling of porcine transmissible gastroenteritis virus of sows as well as infection of piglets, can further implement purification and effect evaluation of pig transmissible gastroenteritis, and has a very high application value. Meanwhile, the kit has a simple and convenient detecting process, needed instruments and equipment are simple, and detecting time is short; the result is relatively reliable, and the kit is not prone to contamination; the kit has a low technological operation demand, and can be generally popularized in the grass root; quality control is relatively easy, and the kit is easy to standardize.

The invention relates to a preparation method of fructus momordicae concentrated juice. The preparation method comprises the following steps of (1) selecting materials; (2) airing the selected materials; (3) crushing the aired materials; (4) performing cooking; (5) performing filtration; and (6) performing concentration. According to the preparation method of fructus momordicae concentrated juicedisclosed by the invention, the fructus momordicae concentrated juice is wide in raw material sources and low in production cost, the prepared fructus momordicae concentrated juice is good in mouth feel, sweet in flavor, clean, sweet and refreshing, and capable of clearing heat and quenching thirst, has the effects of nourishing throat, nourishing the lungs, resolving phlegm, relieving a cough, invigorating the spleen, regulating the flow of vital energy, promoting digestion, removing food stagnation, promoting appetite, neutralizing the effect of alcoholic drinks, reducing weight, reducing blood lipid, preventing constipation and the like, and has prevention and auxiliary treatment effects on laryngopharyngitis, gastroenteritis, high blood lipid, high blood pressure and heart diseases after being drunk for a long term.

The invention discloses a breeding method for breeding pigs, and relates to the technical field of pig breeding. In the breeding pig breeding process, the breeding pigs in different phases such as piglet phase, reserve swine phase and sow phase are subjected to immunization by various vaccines in different time stages. According to the breeding method, a normative immunization program is provided, so that swine fever, foot-and-mouth diseases, pseudorabies, porcine reproductive and respiratory syndrome, parvovirus, epidemic diarrhea, contagious gastroenteritis porcine rotavirus, epidemic encephalitis B, haemophilus parasuis, porcine circovirus and the like which are common in the piglets, the reserve swines and the sows can be effectively prevented.

The invention discloses Lepidogrammitis herb healthy tea, and is prepared from Lepidogrammitis herb, Utricularia bifida L., wintergreen barberry root and Kyllinga monocephala Rottb according to a certain weight ratio. The healthy tea has efficacies of clearing away heat and toxic materials, relieving exterior syndrome and abating fever, diminishing inflammation, clearing away the lung-heat, inducing mild diaphoresis and relieving a cough, is low in cost, good in effects, reasonable in formula, simple in preparation, easy to store, appropriate for the four seasons, and capable of treating diseases, has healthy effects when people have no diseases, and is convenient in drinking, and suitable for the people who have sore throats, tuberculosis cough, cold, gastroenteritis, diarrhea, pertussis and the like and have good effects, so that the healthy tea is an ideal healthy drink.

The invention discloses a kind of medicinal liquor for curing rheumatism and injuries from falls. The liquor at least includes root of pseudo-ginseng and garden burnet. The more the content of root of pseudo-ginseng and garden burnet, the shorter the marinating time; the contents are garden burnet 120 g, root of pseudo-ginseng 40g in 1000 ml distilled spirit. The medicinal liquor can cure fracture, rheumatoid arthritis, atrophic arthritis, nerve numb, muscle fiber inflammation, tendon injury, chronic gastroenteritis, emptysis, haematemesis, stool blood, hematuria, hemorrhoids bleeding, functional venter bleeding, diarrhea and so on.

The present invention discloses a preparation method and a new usage of a pharmaceutical preparation for treating enteritis. The preparation method is that: the Chinese mosla herb is distilled by using water vapor or is extracted the volatile oil by refluxing/supercritical extraction, the distilled liquid is preserved in another container for standby; the medicine residues, humifuse euphorbia herb, goldhair hedyotis herb, camphor root and maple leaves are treated by water extraction for 2 to 3 times, 1 to 4 hours for each time, the water extraction liquid is concentrated to the relative density of 1.04 to 1.35 at 80 DEG C, the excipients are added, then drying and smashing are carried out, the distilled liquid of Chinese mosla herb is used for granulation or the clear paste is dried at low temperature to prepare the dry extract, then the smashing is carried out, the excipients are added, the distilled liquid is used for granulation and drying, the volatile oil of the Chinese mosla herb and the camphor or camphor oil which is dissolved and diluted by ethanol are sprayed in, so as to prepare the granules by granulation or prepare the tablets by compression/ prepare the capsules. The present invention also discloses the new usage of the preparation for treating enteritis prepared by the method in the treatment of constipation.