69 results about "Bezoar" patented technology

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a bezoar intermediate and a preparation method of bezoar. The preparation method comprises the following steps: mixing preparation raw materials and biochemical reaction liquid to obtain a mixed raw material solution; the preparation raw materials comprise bilirubin, organic active raw materials and vitamins, the biochemical reaction liquid is sterile purified water, and the bilirubin comprises bilirubin or biliverdin; and reacting the mixed raw material solution to obtain the bezoar intermediate. The content of combined bilirubin in the bezoar intermediate prepared by the preparation method provided by the invention is 75%-85%, the content of combined biliverdin is 73%-83%, and the yield is 83%-87%. According to the method, the poisoning and destroying effects of mycotoxin on the content, the yield, the biological activity and the oxidation resistance of the bezoar intermediate are effectively eliminated. The content, the yield, the oxidation resistance and the biological activity of the bezoar intermediate are greatly improved, the performance is stable, and the quality of bezoar is ensured.

The invention provides a Mongolian medicine composition for treating hepatitis B and a preparation method of the Mongolian medicine composition. The composition is prepared from the following components in parts by weight: 75 to 85 parts of saffron, 75 to 85 parts of trogopterus dung, 65 to 75 parts of gardenia jasminoides, 45 to 55 parts of cortex phellodendri, 45 to 55 parts of dianthus chinensis, 55 to 65 parts of gypsum, 55 to 65 parts of scabiosa, 55 to 65 parts of calamine, 55 to 65 parts of caulis akebiae, 25 to 35 parts of rhizoma coptidis, 25 to 35 parts of fructus gardeniae, 25 to 35 parts of calomel, 25 to 35 parts of bunge corydalis herb, 45 to 55 parts of elecampane, 45 to 55 parts of rose and 55 to 65 parts of calculus bovis factitius. The Mongolian medicine composition for treating hepatitis B is prepared from multiple raw materials, meanwhile, the added coptis chinensis and rose flowers are treated through a specific extraction mode and a specific fermentation mode, and the prepared Mongolian medicine composition for treating hepatitis B has an excellent hepatitis B treatment effect.

The application relates to the technical field of biological pharmacy, and discloses a method for preparing high-bionic bovine bezoar by using a 3D printing technology and application. The preparation raw material of the high-bionic bovine bezoar comprises the following components: fermented bovine bile, saturated calcium hydroxide, hydroxypropyl methyl cellulose, bilirubin, cholic acid, deoxycholic acid and taurine; wherein the fermented bovine bile is obtained by fermenting bovine bile by using escherichia coli. The high-bionic bovine bezoar prepared by using the preparation raw material to perform 3D printing can be comparable to natural bovine bezoar in terms of components, effects and appearance, the main pharmaceutical components can be adjusted according to actual requirements, the production cost of the bovine bezoar can be greatly reduced, and the demand of people for the bovine bezoar can be met.

This invention provides the application of Jiawei Xihuang Pill (MXHP) in the preparation of drugs for the prevention and / or treatment of malignant tumors. This invention also provides the application of Jiawei Xihuang Pill in combination with chemotherapy drugs for the prevention and / or treatment of malignant tumors, particularly glioblastoma multiforme (GBM), wherein the active ingredients of Jiawei Xihuang Pill are prepared from bezoar, musk, vinegar-processed frankincense, vinegar-processed myrrh, and Panax notoginseng root extract in a weight ratio of 14-16:14-16:545-555:545-555:245-255. MXHP can synergistically inhibit the growth of glioblastoma in situ and induce tumor cell apoptosis, indicating that MXHP may be a potential therapeutic drug. Furthermore, compared to XHP, MXHP significantly prolongs the median survival of GBM, exhibiting an enhanced anti-GBM effect.

The application belongs to the field of traditional Chinese medicines, and particularly relates to a more low-irritation analgesic ancient prescription microemulsion gel and application thereof in anti-arthritis drugs. The application finds that after water-soluble and fat-soluble components of black plaster prepared from raw frankincense, myrrh, eaglewood, dragon's blood, Strychnos nux-vomica, Panax notoginseng, Oldenlandia diffusa, Venenum Bufonis, bovine bezoar, Stachys jamaicensis, Platycodon grandiflorum, Hirudo nipponia, Tinea, Solenopsis geminata, Scorpio, scorpio, red lead and sesame oil are extracted, a more low-irritation analgesic ancient prescription microemulsion gel is prepared; compared with the black plaster itself, the analgesic effect of the microemulsion gel is significantly improved, and the microemulsion gel has good anti-inflammatory effect and can be used for preparing anti-arthritis drugs; and the microemulsion gel has reduced toxicity and is more convenient to use.

The invention belongs to the technical field of detection and analysis, and provides an HPLC detection method for taurocholic acid in snake gall bulbus fritilariae liquid, which comprises the following steps: collecting chromatograms of a test sample and a reference substance through HPLC, and judging whether a co-outflow phenomenon exists or not; if the co-outflow phenomenon occurs, interference proportion analysis is carried out, and main interferents are identified; the judged main interferents are removed; carrying out retention analysis to obtain a taurocholic acid retention rate, judging whether the taurocholic acid retention rate accords with expectation or not based on the taurocholic acid retention rate, and if not, judging whether a trailing signal is triggered or not; if the trailing signal is triggered, performing cause analysis to determine the cause of triggering the trailing signal; when the optimal methanol proportion is obtained, a comprehensive detection signal is triggered; if the comprehensive detection signal is triggered, performing comprehensive adaptation analysis of the HPLC detection method to obtain an adaptation index, and evaluating the comprehensive performance of the HPLC detection method according to the adaptation index; and the accuracy of HPLC taurocholic acid detection is effectively improved.

The invention provides a high-fidelity calculus bovis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. According to the technical scheme provided by the invention, the high-fidelity calculus bovis is accurately prepared by taking the mean value of the contents of various components of the natural calculus bovis as a reference and accurately measuring the contents of various components in the raw materials, fully utilizing the original components of the ox bile or ox gall powder, not adding other non-calculus bovis components and combining the existence forms of the components. The problems that existing artificial calculus bovis is greatly different from natural calculus bovis in efficacy, and toxic and side effects are relatively high are solved. The shape, the forming process and the components of the product are highly similar to those of natural calculus bovis; the medicine effect of the calculus bovis is improved, and the production cost is reduced.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

This invention discloses a single-herb bezoar preparation based on an ancient prescription; an enteric-coated bezoar soft capsule and its preparation method. The enteric-coated bezoar soft capsule consists of a bezoar dispersion and an enteric-coated soft capsule shell. A sand mill is used to prepare bezoar powder into micron-sized particles with a particle size ≤100μm. The micron-sized bezoar powder is fully dispersed in a dispersion medium, thereby improving the solubility of bezoar. The single-herb bezoar preparation can improve the problem of inconsistent bezoar content in compound bezoar preparations. The process of preparing the ≤100μm micron-sized bezoar dispersion uses non-metallic grinding equipment, avoiding the catalytic reaction of metal ions during the preparation of micron-sized powder. Low-temperature grinding is also used to avoid denaturation of the heat-sensitive active substances in bezoar, better preserving its biological activity. The enteric-coated formulation prevents the drug from being released in the stomach until it reaches the small intestine, avoiding the precipitation of bilirubin and other bile acids in the acidic environment of the stomach, which would otherwise lead to insoluble solids and poor solubility.

The invention discloses an oxgall-tolerant in-vitro calculus bovis culture transformation strain and application thereof, and belongs to the technical field of calculus bovis culture. The strain is obtained by separating and purifying fresh ox bile, is identified to belong to escherichia coli, has excellent ox bile tolerance, has the characteristics of beta-glucuronidase, high bezoar conversion rate and high safety, and can be used in the production of in-vitro artificial cultivation of bezoar. The strain is a brand new strain for in-vitro calculus bovis transformation, not only enriches a strain resource library of calculus bovis transformation bacteria, but also provides a new thought for deep research of subsequent in-vitro calculus bovis culture.

The invention discloses a method for culturing calculus bovis by utilizing flora, which is characterized in that cattle gall bladders obtained by slaughtering are used as reaction containers, so that the animal welfare problem caused by living body surgery is completely avoided. A plurality of key steps of bilirubin generation, cholesterol conversion, matrix formation and the like are synergistically completed by adopting functional complementary synthetic flora, and the components of the generated product are closer to a complex system of natural calculus bovis. The breeding is carried out in a closed bioreactor with controllable parameters, uncontrollable factors such as individual cattle difference and feeding environment are eliminated, and the product quality is highly stable and uniform. Automatic monitoring and feedback control are achieved in the whole cultivation process, and technological parameters are accurate and adjustable. The used strains are all non-pathogenic or food-grade safe strains, and the whole process is sterile, free of exogenous pathogen pollution and antibiotic residue risks, and high in biological safety.

The invention discloses fermentation equipment, and a preparation production line and a production process of in-vitro cultured calculus bovis, and relates to the field of fermentation equipment. According to the invention, the fermentation tank is provided with the biological detection analyzer capable of detecting the number and the state of the strains in the fermentation tank, and the oxygen supply amount of the oxygen inlet pipe to the fermentation tank can be adjusted according to the detected number and the state of the strains in the fermentation tank, so that the oxygen supply amount in the fermentation tank is always in a suitable range; the influence on the fermentation efficiency and the product quality due to the damage to microbial metabolic balance caused by excessive or insufficient oxygen supply is prevented; fermentation liquid is stirred through the stirring assembly, the gas-liquid contact area is increased, the oxygen transfer rate is increased, and the gas guide assembly and the gas distribution assembly which are communicated with the oxygen inlet pipe are arranged in the stirring assembly, so that when the stirring assembly rotates and stirs in the fermentation tank, the oxygen transfer rate is increased; oxygen conveyed by the oxygen inlet pipe can be uniformly distributed and conveyed into the fermentation tank through the gas guide assembly and the gas distribution assembly, so that the mixing uniformity of fermentation liquor and oxygen is improved.

The invention discloses nanometer calculus bovis gel and a preparation method thereof. The nanometer calculus bovis gel is prepared from calculus bovis and a gel material in a weight ratio of 1: (1000-4000), the calculus bovis is one of natural calculus bovis, calculus bovis cultured in vitro, calculus bovis cultured in vivo and artificial calculus bovis; the gel material is prepared from a gel matrix, a humectant, a pH regulator, a preservative and purified water in a weight ratio of 1: (1-10): (0-3): (0-0.2): (20-50). The preparation method comprises the following steps: S1, dissolving bezoar in a dimethyl sulfoxide solution as an organic phase, dropwise adding the organic phase into an emulsifier-containing water phase under a stirring condition, mixing, continuously stirring, and aging for a certain time to obtain a bezoar nanocrystal solution; and S2, performing high-speed centrifugation and washing on the calculus bovis nanocrystal solution for purification, adding a gel material, and uniformly stirring to obtain the nanometer calculus bovis gel. The chemical composition of the obtained nanometer calculus bovis is not changed in the preparation process, the particle size is uniform, the chemical property is stable, the nanometer calculus bovis is uniformly dispersed in a gel matrix, and the stability is good.

The invention discloses a bezoar quality improving method based on natural pasture environment optimization. According to the method, the internal quality of the calculus bovis is remarkably improved through multi-dimensional cooperative regulation and control. A natural feed basis with balanced nutrition is provided for cattle from soil environment baseline investigation to precise pasture proportioning, and by combining directional cultivation and implantation of genetic engineering strains, the metabolic activity of rumen microorganisms is effectively enhanced, and accumulation of key components for synthesis of calculus bovis is promoted. The linkage application of the intelligent monitoring system and the environment regulation and control device optimizes the living environment of cattle in real time, and cooperates with innovative intervention means such as lavender essential oil and music therapy, so that the stress reaction of cattle herds is remarkably reduced, and the efficient operation of liver metabolic pathways is guaranteed. The directional feeding of the functional lick bricks further enhances the conversion efficiency of the calculus bovis precursor substance, the whole-process data tracing ensures the controllability of the quality formation process, and finally the improvement of the content of effective active ingredients in the calculus bovis and the enhancement of the quality stability are realized.

1. Name of the designed product: packing gift box (bezoar). 2. Use of the designed product: the designed product is used for packing medicine. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view.

This invention provides an in vitro cultured bezoar, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The preparation method of this invention involves obtaining a bile-resistant, high-β-glucuronidase-active *Escherichia coli* strain through bile gradient acclimation, which is then combined with *Enterococcus faecalis* to form a functional bacterial community. This community is then cultured in vitro through a three-stage process of proliferation, transformation, and crystallization, followed by gradient calcium ion-induced crystallization and vacuum freeze-drying to obtain in vitro cultured bezoar. This invention aims to solve the problems of low conversion efficiency, uneven crystallization, insufficient content of effective components, and unstable product quality in existing technologies. The obtained in vitro cultured bezoar product has significantly higher bilirubin and conjugated bile acid contents than the standards of the *Chinese Pharmacopoeia*, and the process is stable, controllable, and suitable for industrialization.

The invention discloses a traditional Chinese medicine formula for nourishing liver, improving eyesight, tonifying yang and tonifying kidney, and relates to the technical field of traditional Chinese medicines. The health food is prepared from the following raw materials: 8-18 parts of fructus lycii, 6-11 parts of semen cassiae, 5-8 parts of flos chrysanthemi, 5-7 parts of radix puerariae, 5-6 parts of semen hoveniae, 3-5 parts of fructus hippophae, 3-5 parts of seville orange flower, 3-5 parts of fructus citri sarcodactylis, 4-8 parts of oyster, 4-8 parts of fructus crataegi, 3-12 parts of poria cocos, 3-12 parts of fructus momordicae, 6-8 parts of radix glycyrrhizae and 8-12 parts of fructus corni. The formula covers various medicine properties and effects including nourishing liver to improve eyesight, nourishing kidney yang, benefiting essence and blood, clearing away heat and toxic materials and the like. And the dosage of each medicinal material can be flexibly adjusted according to specific symptoms and physique so as to achieve the optimal treatment effect.

The utility model relates to the field of injectors, in particular to a bezoar strain injector with a leakage-proof design. Comprising a needle cylinder, a piston is installed on the inner wall of the needle cylinder, a needle head is installed at one end of the needle cylinder, an adjusting structure is arranged on one side of the piston and comprises a connecting block, the connecting block is fixedly connected with the piston, a connecting plate is fixedly connected to one side of the connecting block, and a sliding groove is formed in the upper surface of the connecting plate; a positioning block is slidably connected to the inner wall of the sliding groove, a fixing hole is formed in the upper surface of the positioning block, a fixing rod is slidably connected to the inner wall of the connecting plate, and a limiting plate is fixedly connected to one end of the fixing rod. The calculus bovis strain injector with the leakage-proof design has the advantages that the position of the piston can be effectively locked, the piston is prevented from moving due to external factors in the operation, transportation or storage process, the safety of strains is ensured, and the leakage phenomenon is avoided.

The invention discloses application of a safflower liver-clearing thirteen-ingredient pill in preparation of a medicine for repairing liver injury, and belongs to the field of medicines. The safflower liver-clearing thirteen-ingredient pill consists of safflower, cape jasmine, clove, lotus seed, dwarf lilyturf tuber, costus root, medicine terminalia fruit, szechwan chinaberry fruit, red sandalwood, musk, buffalo horn concentrated powder, bezoar and vermilion. The lotus seeds and the costustoot in the safflower liver-clearing thirteen-ingredient pill play a role in repairing liver injury by activating HNF-4alpha, and the ratio of the lotus seeds to the costustoot is 4: (1-3). The research finds that the lotus seed and costus root components in the HHQG can activate the HNF-4alpha, the optimal proportion for activating the HNF-4alpha is preferably selected, and a scientific basis is provided for extracting useful components in medicines and developing natural active small molecular substances.

The utility model discloses a kind of biological cultivation devices of bionic bovine bezoar, comprising: strain separation and purification module, including constant-temperature culture unit and strain low-temperature storage unit;Primary fermentation module, including primary fermentation unit and primary centrifugal unit, the input end of primary fermentation unit is connected with strain low-temperature storage unit, the input end of primary centrifugal unit is connected with primary fermentation unit;Bile pretreatment module, including sterilization unit and bile low-temperature storage unit;Secondary fermentation and composite bilirubin calcium preparation module, including sequentially connected secondary fermentation unit, heating unit and secondary centrifugal unit, the input end of secondary fermentation unit is connected with primary centrifugal unit and bile low-temperature storage unit respectively;Bionic cultivation module, including sequentially connected mixing unit, promote unit and first freeze-drying unit, the input end of mixing unit is connected with secondary centrifugal unit;Bionic shaping module, including shaping unit, the input end of shaping unit is connected with first freeze-drying unit;Spraying module, including sequentially connected spraying unit and second freeze-drying unit, the input end of spraying unit is connected with shaping unit.The utility model simplifies the cultivation process of bionic bovine bezoar, reduces the production cost of bionic bovine bezoar.

The invention discloses a bear gall powder preparation method, an instant high-temperature sterilization process and a vacuum freeze-drying process, and the bear gall powder preparation method has the beneficial effects that the traditional sterilization process can be replaced by the bear gall powder preparation method provided by the invention, and the instant high-temperature sterilization work is carried out by using an ultrahigh-temperature sterilizer; according to the method, the bear gall powder can be sterilized and dried on the premise that active ingredients are not damaged, the retention rate of the bezoar ursodesoxycholic acid is increased to be larger than or equal to 50% and the water content of the bezoar ursodesoxycholic acid is smaller than or equal to 2% under the condition that no additive is added, the original luster of the product can be reserved, and the food safety of the product can be improved.

The application discloses a kind of in-vivo cultivation of bovine calculus bezoar gel and preparation method thereof, it is related to in-vivo cultivation of bovine calculus bezoar technical field.A kind of in-vivo cultivation of bovine calculus bezoar gel, including following mass component: with mass percentage, 0.1%~5% drug depot, 8%~20% high molecular gel skeleton, 0.1%~2% crosslinking agent, 0.1%~2% crosslinking regulator, 50%~65% solvent, 10%~40% crosslinking agent solution;Drug depot exists in the form of β-glucuronidase sustained-release microsphere.The sponge-like porous gel prepared by the application can be injected into the gallbladder of a cow to form a sponge-like bovine calculus bezoar bed through crosslinking reaction, the bovine calculus bezoar bed can slowly release β-glucuronidase, promote the decomposition of conjugated bilirubin into free bilirubin, and finally the free bilirubin forms calcium bilirubinate, which, after combining with mucin, forms bovine calculus bezoar particles, and the particles are enriched by the bovine calculus bezoar bed to form bovine calculus bezoar with high yield and good quality.

The invention discloses an extracorporeal circulation type bovine gall-stone preparation device and method, and relates to the field of artificial calculus bovis preparation. The device comprises a bile storage tank, double peristaltic pumps, a three-way valve, a tubular reaction unit, a constant-temperature heating jacket, a pH adjusting unit, a controller, an optional concentration unit and the like. The method comprises the following steps: preparing bile reaction liquid containing a lithogenic precursor, initializing parameters, starting a device, and circulating the reaction liquid; adjusting the pH value, concentrating to form a supersaturated environment for nucleation, simulating pulse flow at a periodic variable flow rate to promote dynamic growth, and finally harvesting, cleaning and drying. The gallbladder environment is simulated, living body dependence is avoided, the stone forming period is short, the similarity of the product and natural calculus bovis is high, and automatic large-scale production can be achieved.

The invention discloses an external compound preparation for treating skin diseases as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation is prepared from the following raw materials in specific parts by weight: borneol, dipterex tablets, cantharides, huechys sanguinea, ephedra, calculus bovis, dragon's blood, centipedes and garter snakes. The preparation method comprises raw material pretreatment, mixing and blending, and sub-packaging and storage, and is simple in process and easy to operate; the ointment is externally applied to skin lesions every morning and evening, and is suitable for diseases such as eczema, psoriasis, neurodermatitis and the like. Through the synergistic effect of multiple components, the comprehensive curative effects of clearing heat, detoxifying, dredging collaterals, relieving itching and promoting tissue regeneration are achieved, the traditional Chinese medicine composition has the advantages of being rapid in effect taking, convenient to externally use and small in side effect, the problems that an existing skin disease treatment medicine is large in side effect and slow in effect taking are solved, and a new effective scheme is provided for clinical treatment of skin diseases.

The invention discloses a coagulation method for in-vitro enzyme-bred calculus bovis, and belongs to the technical field of preparation of traditional Chinese medicine raw materials. According to the method, after repeated research and testing, the key point of'core coagulation 'is implanted into'enzyme breeding', so that the yellowing time of the in-vitro enzyme-bred calculus bovis is shortened, complete transfer of components in bile is ensured, and the yield is greatly improved and can reach 1.5-2.0%. The technical problems that in the prior art, the settling speed is low, the enzyme breeding time is long, the yield is low, and effective components are not transferred thoroughly are solved. The invention aims to ensure the completeness of components contained in the in-vitro enzyme-cultured calculus bovis, so that the medicine property and the medicine effect of the calculus bovis are closer to those of natural calculus bovis.

This invention discloses a high-activity-content *Tai Sui* water. The invention involves immersing fresh *Tai Sui* in an aqueous solution of fructooligosaccharides, or further adding an appropriate amount of compound enzymes to the immersion solution for fermentation, and finally filtering and sterilizing to obtain the *Tai Sui* water of this invention. The preparation process of this invention is short-cycle, environmentally friendly, and yields *Tai Sui* water with a high content of active ingredients.

1. Name of the designed product: packaging box (Mushixiangniuhuang pills). 2. Use of the designed product: for packaging medicine. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

The present application relates to the technical field of medicine, in particular to a preparation method and application of Yixin pill. The preparation method comprises the following steps: crushing red ginseng, safflower, Fupian (black Shunpian) and Sanqi, and performing complex enzyme hydrolysis, inactivation, to obtain extract A; crushing toad venom and pearl powder, mixing with ox horn tip powder, performing papain hydrolysis, inactivation, to obtain extract B; combining extract A and extract B, adding beta-glucosidase for hydrolysis, inactivation, filtration, concentrating and drying the filtrate, to obtain extract C; fully dissolving artificial cow-bezoar, artificial musk, borneol and benzoin, adding sodium alginate solution and chitosan solution, stirring, freeze-drying, to obtain component D; mixing extract C and component D, adding starch, stirring, pill-making, drying, activated carbon coating, polishing, to obtain Yixin pill. The Yixin pill prepared by the preparation method has better effects of reducing blood pressure and blood lipid than Yixin pill prepared by the pharmacopoeia method and other comparative examples.

This invention belongs to the field of traditional Chinese medicine technology, specifically disclosing a topical traditional Chinese medicine composition, preparation method, and application for treating anorectal diseases. The topical traditional Chinese medicine composition is made from the following raw materials in parts by weight: 50-200 parts of Polygonum cuspidatum, 0.1-5 parts of musk, 0.1-5 parts of bezoar, 10-80 parts of borneol, 50-200 parts of pearl powder, 5-40 parts of alum, 1-30 parts of borax, 0.1-5 parts of pearl, and 0.1-5 parts of amber. The topical traditional Chinese medicine composition provided by this invention, through optimization of the formula, has good efficacy for anorectal diseases and postoperative complications. Furthermore, the preparation method is simple, controllable, and easy to scale up, showing good market application prospects.