49 results about "Bezoar" patented technology

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a bezoar intermediate and a preparation method of bezoar. The preparation method comprises the following steps: mixing preparation raw materials and biochemical reaction liquid to obtain a mixed raw material solution; the preparation raw materials comprise bilirubin, organic active raw materials and vitamins, the biochemical reaction liquid is sterile purified water, and the bilirubin comprises bilirubin or biliverdin; and reacting the mixed raw material solution to obtain the bezoar intermediate. The content of combined bilirubin in the bezoar intermediate prepared by the preparation method provided by the invention is 75%-85%, the content of combined biliverdin is 73%-83%, and the yield is 83%-87%. According to the method, the poisoning and destroying effects of mycotoxin on the content, the yield, the biological activity and the oxidation resistance of the bezoar intermediate are effectively eliminated. The content, the yield, the oxidation resistance and the biological activity of the bezoar intermediate are greatly improved, the performance is stable, and the quality of bezoar is ensured.

This invention provides the application of Jiawei Xihuang Pill (MXHP) in the preparation of drugs for the prevention and / or treatment of malignant tumors. This invention also provides the application of Jiawei Xihuang Pill in combination with chemotherapy drugs for the prevention and / or treatment of malignant tumors, particularly glioblastoma multiforme (GBM), wherein the active ingredients of Jiawei Xihuang Pill are prepared from bezoar, musk, vinegar-processed frankincense, vinegar-processed myrrh, and Panax notoginseng root extract in a weight ratio of 14-16:14-16:545-555:545-555:245-255. MXHP can synergistically inhibit the growth of glioblastoma in situ and induce tumor cell apoptosis, indicating that MXHP may be a potential therapeutic drug. Furthermore, compared to XHP, MXHP significantly prolongs the median survival of GBM, exhibiting an enhanced anti-GBM effect.

The application belongs to the field of traditional Chinese medicines, and particularly relates to a more low-irritation analgesic ancient prescription microemulsion gel and application thereof in anti-arthritis drugs. The application finds that after water-soluble and fat-soluble components of black plaster prepared from raw frankincense, myrrh, eaglewood, dragon's blood, Strychnos nux-vomica, Panax notoginseng, Oldenlandia diffusa, Venenum Bufonis, bovine bezoar, Stachys jamaicensis, Platycodon grandiflorum, Hirudo nipponia, Tinea, Solenopsis geminata, Scorpio, scorpio, red lead and sesame oil are extracted, a more low-irritation analgesic ancient prescription microemulsion gel is prepared; compared with the black plaster itself, the analgesic effect of the microemulsion gel is significantly improved, and the microemulsion gel has good anti-inflammatory effect and can be used for preparing anti-arthritis drugs; and the microemulsion gel has reduced toxicity and is more convenient to use.

The invention belongs to the technical field of detection and analysis, and provides an HPLC detection method for taurocholic acid in snake gall bulbus fritilariae liquid, which comprises the following steps: collecting chromatograms of a test sample and a reference substance through HPLC, and judging whether a co-outflow phenomenon exists or not; if the co-outflow phenomenon occurs, interference proportion analysis is carried out, and main interferents are identified; the judged main interferents are removed; carrying out retention analysis to obtain a taurocholic acid retention rate, judging whether the taurocholic acid retention rate accords with expectation or not based on the taurocholic acid retention rate, and if not, judging whether a trailing signal is triggered or not; if the trailing signal is triggered, performing cause analysis to determine the cause of triggering the trailing signal; when the optimal methanol proportion is obtained, a comprehensive detection signal is triggered; if the comprehensive detection signal is triggered, performing comprehensive adaptation analysis of the HPLC detection method to obtain an adaptation index, and evaluating the comprehensive performance of the HPLC detection method according to the adaptation index; and the accuracy of HPLC taurocholic acid detection is effectively improved.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

This invention discloses a single-herb bezoar preparation based on an ancient prescription; an enteric-coated bezoar soft capsule and its preparation method. The enteric-coated bezoar soft capsule consists of a bezoar dispersion and an enteric-coated soft capsule shell. A sand mill is used to prepare bezoar powder into micron-sized particles with a particle size ≤100μm. The micron-sized bezoar powder is fully dispersed in a dispersion medium, thereby improving the solubility of bezoar. The single-herb bezoar preparation can improve the problem of inconsistent bezoar content in compound bezoar preparations. The process of preparing the ≤100μm micron-sized bezoar dispersion uses non-metallic grinding equipment, avoiding the catalytic reaction of metal ions during the preparation of micron-sized powder. Low-temperature grinding is also used to avoid denaturation of the heat-sensitive active substances in bezoar, better preserving its biological activity. The enteric-coated formulation prevents the drug from being released in the stomach until it reaches the small intestine, avoiding the precipitation of bilirubin and other bile acids in the acidic environment of the stomach, which would otherwise lead to insoluble solids and poor solubility.

The invention discloses a method for culturing calculus bovis by utilizing flora, which is characterized in that cattle gall bladders obtained by slaughtering are used as reaction containers, so that the animal welfare problem caused by living body surgery is completely avoided. A plurality of key steps of bilirubin generation, cholesterol conversion, matrix formation and the like are synergistically completed by adopting functional complementary synthetic flora, and the components of the generated product are closer to a complex system of natural calculus bovis. The breeding is carried out in a closed bioreactor with controllable parameters, uncontrollable factors such as individual cattle difference and feeding environment are eliminated, and the product quality is highly stable and uniform. Automatic monitoring and feedback control are achieved in the whole cultivation process, and technological parameters are accurate and adjustable. The used strains are all non-pathogenic or food-grade safe strains, and the whole process is sterile, free of exogenous pathogen pollution and antibiotic residue risks, and high in biological safety.

The invention discloses nanometer calculus bovis gel and a preparation method thereof. The nanometer calculus bovis gel is prepared from calculus bovis and a gel material in a weight ratio of 1: (1000-4000), the calculus bovis is one of natural calculus bovis, calculus bovis cultured in vitro, calculus bovis cultured in vivo and artificial calculus bovis; the gel material is prepared from a gel matrix, a humectant, a pH regulator, a preservative and purified water in a weight ratio of 1: (1-10): (0-3): (0-0.2): (20-50). The preparation method comprises the following steps: S1, dissolving bezoar in a dimethyl sulfoxide solution as an organic phase, dropwise adding the organic phase into an emulsifier-containing water phase under a stirring condition, mixing, continuously stirring, and aging for a certain time to obtain a bezoar nanocrystal solution; and S2, performing high-speed centrifugation and washing on the calculus bovis nanocrystal solution for purification, adding a gel material, and uniformly stirring to obtain the nanometer calculus bovis gel. The chemical composition of the obtained nanometer calculus bovis is not changed in the preparation process, the particle size is uniform, the chemical property is stable, the nanometer calculus bovis is uniformly dispersed in a gel matrix, and the stability is good.

1. Name of the designed product: packing gift box (bezoar). 2. Use of the designed product: the designed product is used for packing medicine. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view.

This invention provides an in vitro cultured bezoar, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The preparation method of this invention involves obtaining a bile-resistant, high-β-glucuronidase-active *Escherichia coli* strain through bile gradient acclimation, which is then combined with *Enterococcus faecalis* to form a functional bacterial community. This community is then cultured in vitro through a three-stage process of proliferation, transformation, and crystallization, followed by gradient calcium ion-induced crystallization and vacuum freeze-drying to obtain in vitro cultured bezoar. This invention aims to solve the problems of low conversion efficiency, uneven crystallization, insufficient content of effective components, and unstable product quality in existing technologies. The obtained in vitro cultured bezoar product has significantly higher bilirubin and conjugated bile acid contents than the standards of the *Chinese Pharmacopoeia*, and the process is stable, controllable, and suitable for industrialization.

The invention discloses a traditional Chinese medicine formula for nourishing liver, improving eyesight, tonifying yang and tonifying kidney, and relates to the technical field of traditional Chinese medicines. The health food is prepared from the following raw materials: 8-18 parts of fructus lycii, 6-11 parts of semen cassiae, 5-8 parts of flos chrysanthemi, 5-7 parts of radix puerariae, 5-6 parts of semen hoveniae, 3-5 parts of fructus hippophae, 3-5 parts of seville orange flower, 3-5 parts of fructus citri sarcodactylis, 4-8 parts of oyster, 4-8 parts of fructus crataegi, 3-12 parts of poria cocos, 3-12 parts of fructus momordicae, 6-8 parts of radix glycyrrhizae and 8-12 parts of fructus corni. The formula covers various medicine properties and effects including nourishing liver to improve eyesight, nourishing kidney yang, benefiting essence and blood, clearing away heat and toxic materials and the like. And the dosage of each medicinal material can be flexibly adjusted according to specific symptoms and physique so as to achieve the optimal treatment effect.

The utility model relates to the field of injectors, in particular to a bezoar strain injector with a leakage-proof design. Comprising a needle cylinder, a piston is installed on the inner wall of the needle cylinder, a needle head is installed at one end of the needle cylinder, an adjusting structure is arranged on one side of the piston and comprises a connecting block, the connecting block is fixedly connected with the piston, a connecting plate is fixedly connected to one side of the connecting block, and a sliding groove is formed in the upper surface of the connecting plate; a positioning block is slidably connected to the inner wall of the sliding groove, a fixing hole is formed in the upper surface of the positioning block, a fixing rod is slidably connected to the inner wall of the connecting plate, and a limiting plate is fixedly connected to one end of the fixing rod. The calculus bovis strain injector with the leakage-proof design has the advantages that the position of the piston can be effectively locked, the piston is prevented from moving due to external factors in the operation, transportation or storage process, the safety of strains is ensured, and the leakage phenomenon is avoided.

The invention discloses application of a safflower liver-clearing thirteen-ingredient pill in preparation of a medicine for repairing liver injury, and belongs to the field of medicines. The safflower liver-clearing thirteen-ingredient pill consists of safflower, cape jasmine, clove, lotus seed, dwarf lilyturf tuber, costus root, medicine terminalia fruit, szechwan chinaberry fruit, red sandalwood, musk, buffalo horn concentrated powder, bezoar and vermilion. The lotus seeds and the costustoot in the safflower liver-clearing thirteen-ingredient pill play a role in repairing liver injury by activating HNF-4alpha, and the ratio of the lotus seeds to the costustoot is 4: (1-3). The research finds that the lotus seed and costus root components in the HHQG can activate the HNF-4alpha, the optimal proportion for activating the HNF-4alpha is preferably selected, and a scientific basis is provided for extracting useful components in medicines and developing natural active small molecular substances.

The utility model discloses a kind of biological cultivation devices of bionic bovine bezoar, comprising: strain separation and purification module, including constant-temperature culture unit and strain low-temperature storage unit;Primary fermentation module, including primary fermentation unit and primary centrifugal unit, the input end of primary fermentation unit is connected with strain low-temperature storage unit, the input end of primary centrifugal unit is connected with primary fermentation unit;Bile pretreatment module, including sterilization unit and bile low-temperature storage unit;Secondary fermentation and composite bilirubin calcium preparation module, including sequentially connected secondary fermentation unit, heating unit and secondary centrifugal unit, the input end of secondary fermentation unit is connected with primary centrifugal unit and bile low-temperature storage unit respectively;Bionic cultivation module, including sequentially connected mixing unit, promote unit and first freeze-drying unit, the input end of mixing unit is connected with secondary centrifugal unit;Bionic shaping module, including shaping unit, the input end of shaping unit is connected with first freeze-drying unit;Spraying module, including sequentially connected spraying unit and second freeze-drying unit, the input end of spraying unit is connected with shaping unit.The utility model simplifies the cultivation process of bionic bovine bezoar, reduces the production cost of bionic bovine bezoar.

The invention discloses an extracorporeal circulation type bovine gall-stone preparation device and method, and relates to the field of artificial calculus bovis preparation. The device comprises a bile storage tank, double peristaltic pumps, a three-way valve, a tubular reaction unit, a constant-temperature heating jacket, a pH adjusting unit, a controller, an optional concentration unit and the like. The method comprises the following steps: preparing bile reaction liquid containing a lithogenic precursor, initializing parameters, starting a device, and circulating the reaction liquid; adjusting the pH value, concentrating to form a supersaturated environment for nucleation, simulating pulse flow at a periodic variable flow rate to promote dynamic growth, and finally harvesting, cleaning and drying. The gallbladder environment is simulated, living body dependence is avoided, the stone forming period is short, the similarity of the product and natural calculus bovis is high, and automatic large-scale production can be achieved.

The invention discloses an external compound preparation for treating skin diseases as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation is prepared from the following raw materials in specific parts by weight: borneol, dipterex tablets, cantharides, huechys sanguinea, ephedra, calculus bovis, dragon's blood, centipedes and garter snakes. The preparation method comprises raw material pretreatment, mixing and blending, and sub-packaging and storage, and is simple in process and easy to operate; the ointment is externally applied to skin lesions every morning and evening, and is suitable for diseases such as eczema, psoriasis, neurodermatitis and the like. Through the synergistic effect of multiple components, the comprehensive curative effects of clearing heat, detoxifying, dredging collaterals, relieving itching and promoting tissue regeneration are achieved, the traditional Chinese medicine composition has the advantages of being rapid in effect taking, convenient to externally use and small in side effect, the problems that an existing skin disease treatment medicine is large in side effect and slow in effect taking are solved, and a new effective scheme is provided for clinical treatment of skin diseases.

This invention discloses a high-activity-content *Tai Sui* water. The invention involves immersing fresh *Tai Sui* in an aqueous solution of fructooligosaccharides, or further adding an appropriate amount of compound enzymes to the immersion solution for fermentation, and finally filtering and sterilizing to obtain the *Tai Sui* water of this invention. The preparation process of this invention is short-cycle, environmentally friendly, and yields *Tai Sui* water with a high content of active ingredients.

The present application relates to the technical field of medicine, in particular to a preparation method and application of Yixin pill. The preparation method comprises the following steps: crushing red ginseng, safflower, Fupian (black Shunpian) and Sanqi, and performing complex enzyme hydrolysis, inactivation, to obtain extract A; crushing toad venom and pearl powder, mixing with ox horn tip powder, performing papain hydrolysis, inactivation, to obtain extract B; combining extract A and extract B, adding beta-glucosidase for hydrolysis, inactivation, filtration, concentrating and drying the filtrate, to obtain extract C; fully dissolving artificial cow-bezoar, artificial musk, borneol and benzoin, adding sodium alginate solution and chitosan solution, stirring, freeze-drying, to obtain component D; mixing extract C and component D, adding starch, stirring, pill-making, drying, activated carbon coating, polishing, to obtain Yixin pill. The Yixin pill prepared by the preparation method has better effects of reducing blood pressure and blood lipid than Yixin pill prepared by the pharmacopoeia method and other comparative examples.

This invention belongs to the field of traditional Chinese medicine technology, specifically disclosing a topical traditional Chinese medicine composition, preparation method, and application for treating anorectal diseases. The topical traditional Chinese medicine composition is made from the following raw materials in parts by weight: 50-200 parts of Polygonum cuspidatum, 0.1-5 parts of musk, 0.1-5 parts of bezoar, 10-80 parts of borneol, 50-200 parts of pearl powder, 5-40 parts of alum, 1-30 parts of borax, 0.1-5 parts of pearl, and 0.1-5 parts of amber. The topical traditional Chinese medicine composition provided by this invention, through optimization of the formula, has good efficacy for anorectal diseases and postoperative complications. Furthermore, the preparation method is simple, controllable, and easy to scale up, showing good market application prospects.

The invention provides a yellow-causing bacterium capable of being used for in-vitro culture of calculus bovis and application of the yellow-causing bacterium, and relates to the technical field of microorganisms, the yellow-causing bacterium is obtained by separating and culturing natural yak bile, and not only has better yellow-producing capacity, but also has higher tolerance to bile. The yellow-causing bacteria are obtained by screening through an optimized screening strategy, gram-positive yellow-causing bacteria can be obtained through the screening strategy, and the screening range of potential yellow-causing bacteria is expanded. The xanthogenic bacterium can directly take bile of natural yaks, cattle, buffalo and other bovine animals as a raw material to perform in-vitro calculus bovis culture, so that the product is closer to the formation process of natural calculus bovis, has the potential of being developed into a natural calculus bovis substitute, and has a better application prospect in the aspect of in-vitro calculus bovis culture.

A method for screening and efficiently cultivating bezoar strains belongs to the field of bezoar strain screening, and comprises the following steps: step 1, inoculating a culture medium with low-pathogenicity Escherichia coli for culturing, comparing and observing, picking out single colonies with good growth vigor for subculture, and preserving the strains after culture; 2, taking the primarily screened preserved strain, reviving in four regions of an LB solid culture medium by marking lines, selecting a single colony, putting the single colony into a first gradient liquid bile culture medium, culturing, measuring OD600, and if OD600gt is detected, determining that the single colony is not detected; and 0.6, the strain can be placed in a liquid bile culture medium of the next gradient, and the strain which can grow in the three gradients can be inoculated into fresh oxgall for culture. The method has the advantages that the escherichia coli strain with high beta-glucuronidase activity for efficiently cultivating calculus bovis can be obtained, in-vitro cultivation verifies that the escherichia coli strain has bile resistance, calculus bovis analogues can be obtained, and the physicochemical properties of the calculus bovis analogues are similar to those of natural calculus bovis.

The invention discloses a method for in-vitro cultivation of natural-quality calculus bovis by adopting biological enzyme, and relates to the technical field of traditional Chinese medicine preparation, and the method comprises the following steps: S1, adding zinc-manganese alloy nanoparticles (the atomic ratio of zinc to manganese is 1: 0.5) and an EGCG-rosmarinic acid-tea polyphenol ternary compound (the mass ratio of the three components is 1: 1.2: 0.8) into fresh ox bile according to the mass ratio of 1000: (0.8-1.2): (3.0-4.5), and uniformly stirring; and magnetically stirring at 55-65 DEG C for 30-35 minutes to form a nanoscale redox microenvironment, thereby obtaining the pretreated bile. According to the invention, the first controllable, standardized and green calculus bovis in-vitro culture system in the world is constructed through three-dimensional technical innovation of nano material synergism, multienzyme engineering coupling and supercritical purification.

The utility model discloses a kind of integrated solid-liquid separation function's cow-bezoar antidotal tablet chinese herbal medicine circulation extraction device, including support two, two described support upper end between fixedly connected with device body, the device body top is fixed with pressurizing assembly, the device body both sides are fixed with circulation assembly, the circulation assembly is below pressurizing assembly, the pressurizing assembly includes hydraulic cylinder, the hydraulic cylinder outer wall is equipped with positioning sleeve.The utility model discloses a kind of integrated solid-liquid separation function's cow-bezoar antidotal tablet chinese herbal medicine circulation extraction device, by setting pressurizing assembly, utilize compression board to compress chinese herbal medicine, compared with traditional manual compression, can make chinese herbal medicine compression more sufficient and uniform, improve the efficiency and compression rate of extraction, more efficient and fast, by setting circulation assembly, the filtered extract that deposition in filter plate below can be extracted in multiple circulation, make extract more clean, greatly improve the efficiency and effect of filtration.

The invention belongs to the technical field of biological fermentation, and particularly discloses a fermentation device for in-vitro cultivation of calculus bovis, the fermentation device comprises a fermentation tank, the top of the fermentation tank is fixedly connected with a connecting column pipe, the top of the connecting column pipe is detachably connected with a buffer tank, the buffer tank can buffer a material, and the buffer tank is connected with the fermentation tank. The outer wall of the buffer tank is fixedly connected with a plurality of ultraviolet sterilizing lamps, the ultraviolet sterilizing lamps are used for sterilizing materials, the top of the buffer tank is provided with a power mechanism, the power mechanism provides power, the buffer tank is internally provided with a dredging and discharging mechanism, and the dredging and discharging mechanism provides a mounting position. Through the arrangement of the sealed discharging mechanism, the solid powder feeding mechanism and the liquid feeding mechanism, relative sealing of the interior of the fermentation tank can be kept no matter liquid materials or solid materials are added, so that the materials in the fermentation tank can be prevented from being polluted by infectious microbes, and the risk of fermentation failure is reduced; therefore, the in-vitro calculus bovis cultivation cost is reduced.

The present application belongs to the technical field of artificial cow-bezoar processing, and particularly relates to a horizontal spiral ribbon mixer for artificial cow-bezoar. The horizontal spiral ribbon mixer comprises a mixing cylinder and a stirring mechanism, wherein the stirring mechanism is provided with stirring teeth and functional teeth; the functional teeth have a first state parallel to the stirring axis and a second state perpendicular to the stirring axis; and a monitoring mechanism is arranged to acquire at least torque parameters of the stirring mechanism in a lower half zone phase, dust concentration parameters of an upper half zone of the mixing cylinder, and real-time material temperature parameters of the functional teeth. The problem of uneven mixing and material waste caused by the adhesion and suspension of light medicine powder is effectively solved.

The application provides a laryngology blowing sore powder and a preparation method and application thereof. The blowing sore powder is a three-stage slow-release microsphere with a core-shell structure. The preparation method comprises the following steps: S1. loading Fritillaria cirrhosa Hance, pollen typhae and radix angelicae dahuricae on modified hydroxyapatite-calcined borax composite micro-nuclei to form an inner long-acting slow-release core; S2. coating the inner micro-nuclei with an enzyme-responsive methacrylated gelatin / poly-epsilon-caprolactone double-network composite gel, and loading salt cortex phellodendri, rhizoma coptidis, rhizoma anemarrhenae, artificial bovine bezoar and natural borneol to form a middle medium-release drug layer; and S3. constructing an outer micro-needle anchoring layer on the surface of the microsphere by freeze-etching chitosan quaternary ammonium salt embedded with menthol. The microsphere realizes physical anchoring and electrostatic adsorption through the outer micro-needle, prolongs the retention time, the middle gel is degraded in response to the ulcer inflammation microenvironment, and releases anti-inflammatory and antibacterial components; and the inner micro-nuclei slowly release repair components to promote mucosal healing, so that targeted retention and graded release of drugs are realized, and the drug efficacy utilization rate is improved.

The invention relates to the technical field of livestock breeding, and particularly discloses a traditional Chinese medicine composition for improving semen quality of stud rams and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from raspberry, salted semen cuscutae, herba epimedii, wine-processed cistanche deserticola, fructus amomi, golden cypress, cinnamon and honey-fried licorice root according to a specific proportion. According to the traditional Chinese medicine composition provided by the invention, through continuous feeding, the ejaculation amount and sperm motility of the breeding rams can be remarkably improved, the semen quality and sexual desire performance are improved, and the service life of the breeding rams is prolonged; natural medicinal materials are adopted, endocrine disorder or drug resistance is not prone to being caused, and the composition is suitable for being popularized and used in large-scale farms and meets the requirements for green breeding and sustainable development.

The invention discloses an in-vivo calculus bovis cultivation method, and relates to the technical field of calculus bovis cultivation, and the method comprises the following steps: constructing a bile component and a physiological environment suitable for calculus bovis formation; carrying out seed nucleus induction in the gall bladder and regulating and controlling crystallization nucleation conditions; bile power regulation and systolic rhythm control are carried out; the calculus bovis is gathered according to the set direction and density by applying a directional deposition regulation and control means and a magnetic field guiding mode; based on maturity monitoring and structure evaluation results, the growth stage of bezoar is determined, and mild and lossless collection is carried out. According to the method, the proper bile biochemical environment is constructed, controllable nucleation is induced, the dynamic rhythm of the gall bladder is adjusted, oriented deposition is achieved by applying the magnetic field, accurate maturity evaluation is achieved in combination with multi-modal monitoring, controllable cultivation of calculus bovis in the body is achieved, the nucleation success rate and deposition uniformity can be remarkably improved, and the method is suitable for large-scale popularization and application. The forming direction, density and bedding structure of the calculus bovis are controllable, the growth period is greatly shortened, and the yield of high-quality calculus bovis is improved.

The invention discloses an efficient and stable in-vitro cultured calculus bovis preparation process, and belongs to the technical field of biological preparation of traditional Chinese medicines. The process comprises the five steps of genetic engineering compound enzyme liquid preparation, bile pretreatment, bionic enzymatic reaction, crystallization purification and quality regulation and control. The core innovation points are as follows: an engineering strain is constructed through a CRISPR-Cas9 technology to obtain a high-activity compound enzyme solution (the activity is greater than or equal to 80U / mL); an AI intelligent control system is adopted to realize accurate regulation and control, the stability fluctuation of the system is less than or equal to + / -0.5%, and the contamination rate is less than or equal to 0.03%; a composite anti-oxidation system and a supercritical COrefining process are matched, so that the oxidation loss of effective components is reduced. The production period is shortened to 3 days, the finished product bilirubin is larger than or equal to 38.2%, the cholic acid is larger than or equal to 6.6%, the product conforms to the 2025 edition pharmacopoeia standard, acute toxicity is extremely low, skin irritation and sensitization are avoided, the industrialization prospect is good, the product quality is high, safety is high, and natural calculus bovis can be effectively replaced to meet clinical requirements.