9 results about "Hirudo" patented technology

1. Name of the product in this design: Packaging Box (Hirudo leech freeze-dried powder). 2. Purpose of this design: For packaging Chinese herbal medicine slices. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

1.The name of the design product: packaging box (hirudo lyophilized powder). 2.The use of the design product: the design product is used for packaging products. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating diabetic nephropathy, which is prepared from the following raw material medicines in parts by weight: 9-45 parts of raw astragalus membranaceus, 9-30 parts of radix rehmanniae, 3-9 parts of radix aconiti lateralis preparata, 6-15 parts of cassia twig, 15-30 parts of mung bean coat, 9-30 parts of serissa serissoide, 9-18 parts of perilla leaf, 9-18 parts of gordon euryale seed, 6-15 parts of cherokee rose fruit, 3-9 parts of rhizoma zingiberis, 3-9 parts of raw rhubarb and 2-6 parts of leech. The invention further provides a preparation and application of the traditional Chinese medicine composition.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

The utility model is suitable for aquatic product breeding technical field provides a kind of leech breeding box, including breeding box;Fixing frame is fixedly installed on the breeding box, multiple hooks are fixedly installed on the fixing frame, multiple the hook is hung for the yarn cage of leech breeding, the yarn cage extends to the breeding box inside;Threaded cylinder, threaded cylinder is rotatably installed on the fixing frame, threaded rod is screwly installed on the threaded cylinder, the bottom end of threaded rod is equipped with cleaning toroid, the outer wall of cleaning toroid is attached with the inner wall of breeding box;Driving mechanism, the driving mechanism is located on the fixing frame, for driving cleaning toroid moves along the inner wall of breeding box.The leech breeding box provided in the scheme reduces artificial intervention, improves cleaning efficiency and reduces the stress reaction to leech, and provides suitable growth environment for leech.

The present invention relates to the technical field of genetic engineering, and in particular, relates to a method for constructing a recombinant hirudin expression vector using algae as chassis cells, and culture. The endogenous promoter beta2-tubulin and RBCS2 intron sequence constitute a key promoter, which is linked to a Hygromycin resistance gene; a 2A peptide self-cleavage sequence is added between a recombinant hirudin gene and the Hygromycin resistance gene, and an HA tag is connected at the terminus. Thus, an algal expression vector is constructed. In order to improve the safety and synthesis yield of the recombinant hirudin and reduce the production cost, an algal expression vector expressing the recombinant hirudin is successfully constructed. The expression vector can significantly improve the activity and yield of the recombinant hirudin.

The present invention belongs to the technical field of genetic engineering. Provided are a recombinant hirudin with tyrosine sulfation, a preparation method therefor and the use thereof. In order to improve the safety and synthesis yield of recombinant hirudin, and reduce the production cost, a recombinant hirudin with tyrosine sulfation is prepared by means of an algae expression vector.

The present invention relates to the field of fermentation production. Provided is a method for acquiring chlamydomonas reinhardtii source recombinant hirudin algae powder by means of a fermentation culture method. The method can effectively multiply the algae source recombinant hirudin.

The application discloses a characteristic spectrum construction method of medicinal leech and its close relative adulterants. The construction method comprises the following steps: a characteristic spectrum of medicinal leech and its close relative adulterants is established by high performance liquid chromatography, including extracting and preparing test sample solutions from medicinal leech and its close relative adulterants respectively; a control sample solution is prepared, and the test sample solution and the control sample solution are detected by high performance liquid chromatography, so that corresponding characteristic spectra are obtained, and medicinal leech and its close relative adulterants can be quickly distinguished. The medicinal leech control sample is selected from medicinal leech or leech of the Hirudinidae family; the close relative adulterants of the medicinal leech are selected from Limnatis humilis, Haemadipsa rills or Haemadipsa japonica. The method is simple and convenient, has the advantages of simple operation, good separation degree, high precision, good repeatability and good stability, and can realize rapid and accurate distinction of medicinal leech, leech and its close relative adulterants by HPLC.