17 results about "Hirudo" patented technology

1. Name of the product in this design: Packaging Box (Hirudo leech freeze-dried powder). 2. Purpose of this design: For packaging Chinese herbal medicine slices. 3. The key design elements of this product are the combination of shape, pattern, and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

The invention relates to a traditional Chinese medicine composition for treating ankylosing spondylitis, a medicine and a preparation method, and relates to the technical field of traditional Chinese medicines. The invention provides a traditional Chinese medicine composition for treating ankylosing spondylitis. The traditional Chinese medicine composition is prepared from the following raw materials: garden balsam stem, herb of Chinese rabdosia, spina gleditsiae, pangolin scales, camellia oleifera, radix angelicae, sargentgloryvine stem, folium artemisiae argyi, herba asari, leech, ficus microcarpa, semiliquidambar cathayensis, radix gleditsiae, sarcandra glabra, fructus liquidambaris and senecio scandens. The traditional Chinese medicine composition has the effects of dredging tendons and activating blood circulation, can deeply permeate into the body of a patient, enables swollen and calcified muscles and bones to recover to be normal, enables the skin color of a diseased region to recover to be normal, has a very good treatment effect on ankylosing spondylitis, is a neutral medicine, can be accepted by wet, cold and damp-heat constitutions, and is free of toxic and side effects. And the method has wide acceptability.

1. The name of this design product: Packaging box (Chinese herbal medicine pieces). 2. Purpose of this design product: used for packaging of Chinese herbal medicines. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the key points of the design: Main view of Design 1. 5. Designate Design 1 as the base design. 6. Other situations that require explanation Other explanations: (1) Design 1 is used for the packaging of broken-wall slices of Herba Lysimachiae; (2) Design 2 is used for the packaging of broken-wall slices of Herba Cistanches; (3) Design 3 is used for the packaging of broken-wall slices of Herba Angelicae Sinensis; (4) Design 4 is used for the packaging of broken-wall slices of Herba Erigerontis; (5) Design 5 is used for the packaging of broken-wall slices of Herba Lysimachiae; (6) Design 6 is used for the packaging of broken-wall slices of Herba Astragali; (7) Design 7 is used for the packaging of broken-wall slices of Herba Ganodermae; (8) Design 8 is used for the packaging of broken-wall slices of Herba Dracaenas; (9) Design 9 is used for the packaging of freeze-dried powder of Herba Hirudo; (10) Design 10 is used for the packaging of freeze-dried bear bile powder.

The application discloses a kind of leech medicinal materials and its original identification thin layer chromatography method, the thin layer chromatography method includes the following steps: (1) the leech medicinal materials to be measured is pretreated to obtain leech medicinal materials test solution, leech medicinal materials are pretreated to obtain leech control medicinal solution;Wherein, the leech is Hirudinidae leech or leech worm;And (2) the leech medicinal materials test solution and the leech control medicinal solution are identified and analyzed by thin layer chromatography, to determine whether the leech to be measured is leech or leech worm;Wherein, the step of thin layer chromatography identification analysis adopts chloroform-methanol as developing agent, the volume ratio of chloroform and methanol is (6-10): about 1;Wherein, the leech to be measured is selected from one or more of the following: leech, leech worm, willow leaf leech, flatworm, light-rubbing golden line leech, eight-eye stone leech, Japanese leech and stick leech.The method is simple in operation, and the required instrument equipment cost is low, and the detection result is intuitive, accurate and easy to identify.

The invention belongs to the field of biological detection, and particularly relates to a marker polypeptide for leech and poecilobdella manillensis species identification, a kit and application of the marker polypeptide. The sequence of the marker polypeptide is as follows: Pep1: ISSIKNLFESDDDDYFFENK, and the sequence of the marker polypeptide is as follows: Pep1: And the Pep2 is QTDLDQVKR. According to the invention, two marker polypeptides for identifying Hirudo nipponia and Hirudo poecilobdella manillensis are obtained through screening, the specificity is strong, and the durability is good; a target ion pair can be stably detected; according to the method, a technical system for species identification and adulteration weight gain identification of the leech medicinal materials is established, the method is high in specificity and sensitivity, the problem of species confusion under the current pharmacopoeia standard is solved, a brand new technical means is provided for leech medicinal material quality supervision, and powerful technical support is provided for improving the quality control level of the leech medicinal materials.

1.The name of the design product: packaging box (hirudo lyophilized powder). 2.The use of the design product: the design product is used for packaging products. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best shows the design points: perspective view. 5.The design claimed contains color.

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating diabetic nephropathy, which is prepared from the following raw material medicines in parts by weight: 9-45 parts of raw astragalus membranaceus, 9-30 parts of radix rehmanniae, 3-9 parts of radix aconiti lateralis preparata, 6-15 parts of cassia twig, 15-30 parts of mung bean coat, 9-30 parts of serissa serissoide, 9-18 parts of perilla leaf, 9-18 parts of gordon euryale seed, 6-15 parts of cherokee rose fruit, 3-9 parts of rhizoma zingiberis, 3-9 parts of raw rhubarb and 2-6 parts of leech. The invention further provides a preparation and application of the traditional Chinese medicine composition.

The invention provides a high-activity hirudin freeze-drying preparation as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) introducing a hirudin gene to a chassis cell to construct a recombinant engineering strain; (2) inoculating the engineering strain into a prepared culture medium, carrying out fermentation culture at a set temperature, after the thallus density reaches a target density, switching to methanol induced expression, and after continuous induction is carried out for a preset time, extracting a fermentation liquid; (3) performing ultrafiltration concentration and chromatographic purification on the obtained fermentation liquor to obtain a purified hirudin solution; and (4) adding a stabilizer into the obtained hirudin solution, pre-freezing to completely cure the hirudin solution, and drying to obtain the target hirudin freeze-dried preparation. According to the invention, the high-activity, high-purity, high-stability and low-cost industrial production of the hirudin freeze-drying preparation is realized through collaborative optimization of genetic engineering strain construction, high-density fermentation, multi-step purification and freeze-drying processes.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to poecilobdella manillensis freeze-dried powder as well as a preparation method and application thereof. The preparation method of the poecilobdella manillensis freeze-dried powder comprises the following steps: (1) taking living poecilobdella manillensis or freezing and melting the living poecilobdella manillensis, and homogenizing to obtain a poecilobdella manillensis sample solution; (2) carrying out water extraction on the poecilobdella manillensis sample liquid; (3) performing fat extraction on the poecilobdella manillensis precipitate subjected to water extraction; and (4) combining the two extracts to prepare freeze-dried powder. The hirudin content of the poecilobdella manillensis freeze-dried powder prepared by combining a water extraction method with a fat extraction method is remarkably higher than that of a commercially available poecilobdella manillensis product, and the poecilobdella manillensis freeze-dried powder has a remarkable effect on treating thrombotic diseases and other vascular diseases.

The utility model is suitable for aquatic product breeding technical field provides a kind of leech breeding box, including breeding box;Fixing frame is fixedly installed on the breeding box, multiple hooks are fixedly installed on the fixing frame, multiple the hook is hung for the yarn cage of leech breeding, the yarn cage extends to the breeding box inside;Threaded cylinder, threaded cylinder is rotatably installed on the fixing frame, threaded rod is screwly installed on the threaded cylinder, the bottom end of threaded rod is equipped with cleaning toroid, the outer wall of cleaning toroid is attached with the inner wall of breeding box;Driving mechanism, the driving mechanism is located on the fixing frame, for driving cleaning toroid moves along the inner wall of breeding box.The leech breeding box provided in the scheme reduces artificial intervention, improves cleaning efficiency and reduces the stress reaction to leech, and provides suitable growth environment for leech.

The application discloses a heart-protecting granule identification and quality control method based on a thin layer chromatography, and belongs to the technical field of drug detection. The thin layer chromatography identification method comprises the following steps: taking astragaloside as a control product to identify astragalus membranaceus, taking chrysophanol and emodin as control products to identify largeleaf rhubarb, taking hirudo medicinal materials as a control product to identify hirudo, taking glycyrrhizin and licorice medicinal materials as control products to identify licorice, taking catalpol as a control product to identify radix rehmanniae, taking salvianolic acid B and salvia miltiorrhiza medicinal materials as control products to identify salvia miltiorrhiza, and taking naringin and neohesperidin as control products to identify aurantii fructus. The application establishes the thin layer chromatography identification method of the heart-protecting granule, adopts a special developing agent and a color developing mode, realizes accurate identification according to the spot color, fluorescence characteristics and other characteristics of the corresponding positions of the test product chromatogram, the control product and the control medicinal material chromatogram, the method is simple in operation, low in detection cost, strong in repeatability and specificity, and can effectively control the quality of the heart-protecting granule production process.

The present invention relates to the technical field of genetic engineering, and in particular, relates to a method for constructing a recombinant hirudin expression vector using algae as chassis cells, and culture. The endogenous promoter beta2-tubulin and RBCS2 intron sequence constitute a key promoter, which is linked to a Hygromycin resistance gene; a 2A peptide self-cleavage sequence is added between a recombinant hirudin gene and the Hygromycin resistance gene, and an HA tag is connected at the terminus. Thus, an algal expression vector is constructed. In order to improve the safety and synthesis yield of the recombinant hirudin and reduce the production cost, an algal expression vector expressing the recombinant hirudin is successfully constructed. The expression vector can significantly improve the activity and yield of the recombinant hirudin.

1. The name of the design product: mask packaging box (hirudin tricholoma face mask). 2. The use of the design product: used for containing and packaging single piece packaged mask. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

The present invention belongs to the technical field of genetic engineering. Provided are a recombinant hirudin with tyrosine sulfation, a preparation method therefor and the use thereof. In order to improve the safety and synthesis yield of recombinant hirudin, and reduce the production cost, a recombinant hirudin with tyrosine sulfation is prepared by means of an algae expression vector.

The present invention relates to the field of fermentation production. Provided is a method for acquiring chlamydomonas reinhardtii source recombinant hirudin algae powder by means of a fermentation culture method. The method can effectively multiply the algae source recombinant hirudin.

The application discloses a characteristic spectrum construction method of medicinal leech and its close relative adulterants. The construction method comprises the following steps: a characteristic spectrum of medicinal leech and its close relative adulterants is established by high performance liquid chromatography, including extracting and preparing test sample solutions from medicinal leech and its close relative adulterants respectively; a control sample solution is prepared, and the test sample solution and the control sample solution are detected by high performance liquid chromatography, so that corresponding characteristic spectra are obtained, and medicinal leech and its close relative adulterants can be quickly distinguished. The medicinal leech control sample is selected from medicinal leech or leech of the Hirudinidae family; the close relative adulterants of the medicinal leech are selected from Limnatis humilis, Haemadipsa rills or Haemadipsa japonica. The method is simple and convenient, has the advantages of simple operation, good separation degree, high precision, good repeatability and good stability, and can realize rapid and accurate distinction of medicinal leech, leech and its close relative adulterants by HPLC.

The application discloses a gel electrophoresis identification method for leeches and leech worms and their close relatives and application of the method, and relates to the technical field of traditional Chinese medicine identification. The method comprises the following steps: (1) pre-treating a leech sample to be detected to obtain a sample solution of the leech sample to be detected, and pre-treating a control medicinal material to obtain a solution of the control medicinal material; wherein the control medicinal material is a leech, a leech worm and / or a close relative and fake product thereof; (2) performing sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel imaging analysis on the sample solution of the leech sample to be detected and the solution of the control medicinal material to obtain a gel electrophoresis spectrum of the leech sample to be detected and a gel electrophoresis spectrum of the control medicinal material; and (3) comparing the above spectra, and determining whether the leech sample to be detected is a leech, a leech worm or a close relative and fake product thereof according to a comparison result; and the leech sample to be detected is selected from one or more of the following: a leech, a leech worm, a limnatis filamentaria, a haemopis japonica and a haemopis sanguinea. The method is intuitive, accurate and easy to identify.