431 results about "Emodin" patented technology

The present invention concerns the use of methods and compositions to provide an improved lipid:emodin formulation for the treatment of leukemias expressing bcr-abl and other cancer with elevated tyrosine kinase activity.

The invention relates to a pesticide compound for preventing plant disease, wherein said compound comprises emodin monomethyl ether and chrysophanol at 1:1-1:9 mass percentages; the compound can be obtained from Polygonaceae and soybean plant Chinese drug or made from manual purification or manual composition. Said compound added with other carrier and agent can be made into liquid, powder, emulsion, etc, whose effective component is 0.1-30%. The invention can generate sub metabolite with significant bacterial resistance.

A method for studying toxicity of drugs by using model organism zebra fish comprises the following steps of: selecting 100 to 200 adult zebra fishes, respectively disposing in bottles containing a solution of 30 to 50 mL, keeping the temperature in the bottles substantially constant at 22 to 25 DEG C, randomly grouping, each group containing 10 to 20 fishes, wherein the solution in one of the groups is pure water (blank), the solutions in other groups are medicinal solutions with different concentrations, and 0.5% to 2% dimethylsulfoxide (DMSO) can be added to assist dissolving a drug that has poor water solubility, when an additional solvent comparison group prepared by adding 0.5% to 2% DMSO into the pure water is needed; recording the death number of zebra fishes in the medicinal solutions of different concentrations within 24 h, and calculating the death rate (%); calculating median lethal dose-LD50 by Bliss method according to the experiment result; and representing the acute toxicity by the value of LD50. According to the method, the LD50s of zebra fish of triptolide, matrine and emodin are respectively 5.39*10<-3>, 112.3 and 1.08*10<3> mug / mL. The method can objectively reflect the toxicity of drugs.

The invention discloses a method for improving the content of resveratrol by converting giant knotweed materials through an immobilized enzyme method. The method comprises the steps of biologically converting a crude extract of the giant knotweed materials with a specific immobilized enzyme to convert resveratrol analogues therein into the resveratrol in a short time and then obtaining the resveratrol with a high purity by extraction and separation technologies. The method can increase the resveratrol content of the giant knotweed materials by 10 to 20 times, solves the problems of overlong conversion time, difficult control on catalytic time as well as increased cost caused by the fact that the enzyme cannot be recovered after the reaction in the similar techniques, and simultaneously obtains over 95 percent of resveratrol and a side-product archen with a certain purity by adopting the separation and refining techniques such as chromatography, crystallization and the like.

The invention relates to a process for extracting anthraquinone compounds from cassia seed which comprises, (1) portion extraction, (2) preparation or mixture non-soluble to 95% ethanol, (3) isolating and purifying the mixture with microporous resin columns, (4) isolating and purifying the ethanol eluate and anthraquinone compounds with silica gel columns, thus obtaining active antihyperglycemic ingredients including emodin-1-O-beta-gentiobioside, chrysophanol-1-O-beta-gentiobioside, physcion-8-O-beta-gentiobioside, and chrysophanol-1-O-beta-D-glucopyranosyl-(1-3)-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranoside.

The invention relates to an emodin derivative and the application in preparing a anti-cancer drug, and the chemical structural formula of the emodin derivative of the invention is as shown in formula I, wherein, R1, R2, R3 are defined as described in the specification; the emodin and the derivative thereon provided by the invention are compounds which are prepared in a way that natural emodin is taken as the matrix, and then structural modification is carried out in accordance with the principles of drug molecules to design out the highly active compounds; experiments proof that the emodin derivative has obvious inhibitory effect on various tumor cells and can be used in preparing cancer-treatment drugs.

A pharmaceutical composition for prevention and treatment of cancer, cardiovascular disease and antioxidation contains resveratol. The new method of process for producing resveratol is extracted resveratol from oil residue and stems of peanuts or stems of other cheap herbs. Derivates of resveratol include procyanidins, polydatin, peceid, anthraglycoside, emodin, chrysophanol and quercetin.

The invention relates to a quality control method of fresh fleece flower root and prepared fleece flower root which comprises the steps as follows: A. standard crude drug powder of fresh fleece flower root or prepared fleece flower root is weighed and dissolved in acetone; the solution undergoes ultrasonic treatment and is then filtered, brought to constant volume and evaporated to dry; obtained dry powder is dissolved in methyl alcohol and the methyl alcohol solution is filtered by Millipore, thereby obtaining standard crude drug solution; fingerprint spectrum of the standard crude drug is then worked out by high performance liquid chromatography; the same method is then employed to establish fingerprint spectrum awaiting measurement of fresh fleece flower root or prepared fleece flower root to be measured; similarity between the fingerprint spectrum awaiting measurement and the standard fingerprint spectrum are then used for deciding whether the crude drug to be measured is qualified. B. contents of two index components, including 2, 3, 4', 5-tetrahydroxystilbene-2-O-beta-D-glucopyranoside and rheum emodin, are measured by high performance liquid chromatography. The quality control method has various judging evidences, thereby not only realizing qualitative detection of major chemical compositions of fresh and prepared fleece flower root, but also measuring contents of the two major components. The quality control method has reliable and precise measurement result, thereby reducing possibility of quality pass rate affected by manual treatment.

The invention relates to an emodin derivative and application thereof in preparing antibacterial agents. The chemical structural formula of the emodin derivative is represented as formula (I), wherein R1, R2, R3 and R4 are -F, -Cl, -Br, -I or -NO2; and R5, R6 and R7 are -H, -CH3, -CH2CH3 and -COCH3. The emodin derivative is a high-activity compound designed by performing structural modification on compounds with natural emodin as a matrix according to the principle of medical molecules. Experiments prove that the emodin derivative has good effects of resisting drug-resistance bacteria and superbacteria, the pharmaceutical effect of the emodin derivative is remarkably better than that of the emodin, and a new choice is provided for clinical medication.

The invention provides an establishment method of a traditional Chinese medicine composite fingerprint, and particularly relates to an establishment method of a Weilikang granule fingerprint. Pure water is added to Weilikang granules, so as to dissolve, and paeoniflorin, naringin, rutin, salvianolic acid B, rosmarinic acid, emodin-8-O-beta-D glucopyranoside and glycyrrhizic acid are used as reference substances, so as to establish the fingerprint by referring to high-performance liquid chromatography. The method is more comprehensive and objective than the current quality standard, and the current quality standard is increased and improved, and more information is provided for the internal quality control of the Weilikang granules.

The invention relates to a method for synthesizing hypericin. In the method, emodin serves as a raw material; emodin anthrone condensation reaction with low yield is optimized by a microwave-assisted synthesis method under the alkaline condition; the microwave heating temperature is 130 to 180 DEG C; and the reaction time is 0.2 to 1 hour. Under the conditions, the yield of the condensation reaction is increased by multiple times compared with that of the conventional method; reaction time is greatly saved; the yield of the reaction at each step is over 80 percent; and the reaction conditionscan meet the kilogram-grade production scale of a targeted compound. The synthetic route and method has the characteristics of high yield, short reaction route, mild reaction conditions, short reaction time, low synthetic cost and the like.

The invention relates to a combined extraction and purification technique for multiple active ingredients of a medicinal plant, in particular to a combined extraction and purification technique for multiple active ingredients of polygonum multiflorum. According to the method, stilbene glucoside, rheum emodin and chrysophanol in the polygonum multiflorum can be maximum extracted from the polygonum multiflorum into a solvent by using 70-80 percent of ethanol as an extraction solvent and are separated from starch in residual slag; water-soluble differences of the stilbene glucoside, the rheum emodin and the chrysophanol are used and water is used as a solvent so that the stilbene glucoside in alcohol extracts can be separated from the rheum emodin and the chrysophanol, and a crude product is obtained; the rheum emodin and the chrysophanol can be respectively separated from the alcohol extracts by successively using 5 percent of sodium carbonate and 5 percent of sodium hydroxide as solvents, and the rheum emodin and chrysophanol crude product can be obtained through an acid precipitation; and the polygonum multiflorum ethanol extract residual slag is washed by using water, and the starch with a purity of 85-87 percent can be obtained through precipitating water washing liquid. The invention has the characteristics of low cost, high efficiency, no pollution and high product purity.

A Chinese medicine 'Tongsheng softgel' is prepared from 17 Chinese-medicinal materials including ledebouriella root, mint, rhubarb, capejasmine fruit, etc, matrix, suspending aid, and lubricant. Its preparing process and its quality control method are also disclosed.

The invention discloses an emodin di-n-octyl quaternary ammonium salt with anti-leukemia activity and a preparation method thereof. The emodin di-n-octyl quaternary ammonium salt is methyl di-n-octyl-[2-(4,5-dihydroxyl-7-methoxyl-9,10-anthraquinonyl) methyl] ammonium salt. Experiments prove that an emodin derivative, i.e. the emodin di-n-octyl quaternary ammonium salt has proliferation inhibition effects of different degrees on four leukemia cell lines K562, NB4, U937 and Jurkat and can be used for preparing a medicament for treating leukemia. The emodin derivative methyl di-n-octyl-[2-(4,5-dihydroxyl-7-methoxyl-9,10-anthraquinonyl) methyl] ammonium bromide has the advantages of simple preparation process, mild preparation conditions and high yield of reaching up to over 50 percent; and in-vitro cancer cell inhibition experiments show that the four leukemia cell lines K562, NB4, U937 and Jurkat can be effectively killed off.

Processes for the separation and purification of polyphenol trans-resveratrol and / or anthraquinone emodin from Polygonum cuspidatum and / or Rumex acetosa, by means of solvent selective extraction. Products obtained by this process present high level of purity; being therefore useful in the preparation of nutraceutical (pharmaceutical and / or food) compositions with antioxidant, anti-inflammatory, antiviral, cardioprotective, neuroprotective, chemoprotective activities, besides protecting against infections and ischemia, treating type 1 and 2 diabetes, reducing obesity and preventing aging. Useful phytomedicines for the same therapeutic activity and prepared from the roots and / or rhizomes of Rumex acetosa or from their fractions are also provided.

The invention relates to a chemical modification method capable of improving the anti-cancer activity of reactive oxygen species (ROS) through improving the producing ability of the ROS of rheum emodin. Experiments prove that a series of rheum emodin hyamines obtained by chemical modification to the rheum emodin have better anti-cancer activity on three kinds of cancer cells such as skin cancer A375, liver cancer HepG2 and stomach cancer AGS, and the anti-cancer activity and the producing ability of the ROS far exceeds those of the rheum emodin; moreover, experiments prove that the producing ability of the ROS and the anti-cancer activity of rheum emodin derivates are in positive correlation. The drug chemically modified by the chemical modification method has a certain selective killing effect on the cancer cells, and has a better prospect for development as an anti-cancer drug.

The invention relates to a preparation method and a quality control method for a compound indigowoad leaf preparation. The preparation method comprises a step of preparing a basic remedy and a step of preparing a corresponding preparation. The basic remedy in the basic remedy preparation comprises the following compositions in part by weight: 360 to 440 parts of indigowoad leaves, 180 to 220 parts of lonicera confusa or honeysuckle flower, 90 to 110 parts of incised notopterygium rhizome, 90 to 110 parts of bistort rhizome, and 90 to 110 parts of rhubarb; the medicinal materials are decocted twice with conventional amount of water for 1 hour each time; the decoctions are mixed and filtered; the filtrate is concentrated to the relative density of between 1.08 and 1.32 at 60 DEG C; ethanol is added into the filtrate to ensure that alcohol content reaches 50 to 60 percent, the mixture is stood and is filtered, the filtrate is subjected to ethanol reclamation and is concentrated to an extract with the relative density of between 1.17 and 1.43 at 60 DEG C for later use; and the corresponding preparation is prepared according to a drug specification. The quality control method comprises the following steps of the identification of contents and the content determination of the contained compositions including the identification of the indigowoad leaves, the identification of the lonicera confusa or honeysuckle flower, the identification of the rhubarb, the identification of the incised notopterygium rhizome, the total content determination of emodin and chrysophanol in the rhubarb, and the content determination of chlorogenic acid. The methods can be applied to preparation of medicinal preparations for treating cold, influenza, parotitis and acute viral hepatitis.

The present invention provides a compound emodin baicalein injection for curing acute pancreatitis and its preparation method. Said invention adopts the combination of ultrasonic wave method and conventional method to respectively extract effective component emodin and baicalein pure products from rhubarb and scutellaria root, then adopts the following steps: adding the emodin and baicalein pure products and required correspondent auxiliary material into hot injection water, using NaOH solution to regulate pH, adding active carbon, heating to foiling, cooling and filtering by using microporous filtration membrane, adding injection water to total quantity, filling and sterilizing for 30 min at 115 deg.C so as to obtain the injection, cold-storing said injection for 1 week, filtering, filling and freeze-drying, sealing so as to obtain the freeze-dried powder injection finished product.

The invention relates to a method for quantitatively detecting polytypes of chrysophanol and emodin in rhubarb and a compound preparation of rhubarb. The method is characterized by integrating methyl extraction and acid hydrolysis into one step; eliminating steps of extraction by poisonous solvents such as trichloromethane and the like, drying by vaporization and the like; and reducing the pre-processing time from original 10 to 15 hours to 0.5 hour, along with high efficiency, low cost and little pollution. By studying components and ratios of the flowing phase, acetonitrile-methyl alcohol-0.1 percent phosphoric acid (the mixing ratio is n 40-45:20-27:38-30) replaces methyl alcohol-0.1 percent phosphoric acid (the mixing ratio is 85:15) as the flowing phase, the problem of difficultly in identifying coating impurity peaks of the emodin is solved and the correctness of the detection result is ensured. The chrysophanol and the emodin are taken as detection indexes, the same flowing phase is used, and the chrysophanol and the emodin in total, ionization and combined anthraqunione in the preparation are detected at the same. The method has the advantages of combining the detection indexes and the efficacy of the preparation for the first time, improving the scientificity and rationality of the quality control indexes, and being suitably used for various preparations containing the rhubarb, along with simpleness, quickness and accuracy.

The invention belongs to the technical field of functional material preparation, and specifically relates to an emodin molecular imprinted titanium dioxide nanoparticle composite film and a preparation method and application thereof. The emodin molecular imprinted titanium dioxide nanoparticle composite film is prepared by taking a cellulose acetate membrane as a basement membrane, emodin as template molecules, dopamine as a functional monomer and a crosslinking agent and combining a nanoparticle surface modification technology and a molecular imprinting polymerization technology. The emodin molecular imprinted titanium dioxide nanoparticle composite membrane prepared by the method has high specific recognition ability and adsorption and separation ability to emodin, and the preparation method is safe, non-toxic, low in energy consumption and easy in operation. The composite membrane can be applied to the selective adsorption and separation of emodin in emodin analogs.

The invention discloses a dyeing method of water insoluble natural pigment on silk fabric. The water insoluble natural pigment is one of oil soluble curcumin, emodin, chrysophanol and oil soluble paprika oleoresin. The method comprises the following steps: step 1: dissolving the water insoluble pigment in an organic solvent to obtain a pigment solution; step 2: adding an emulsifier in the pigment solution to obtain a mixed solution; step 3: stirring the solution while adding deionized water into the mixed solution; after the addition, continuing to stir to obtain an oil-in-water pigment microemulsion liquid; and step 4: dyeing the silk fabric in the pigment microemulsion liquid. The invention overcomes the boundedness that water insoluble natural pigment is unable to dye the silk fabric in water, so as to realize uniform dyed fiber of silk fabric by water insoluble natural pigment, and high fastness.

The invention discloses a rheum emodin double-chain biquaternary ammonium salt with the anti-cancer activity and a preparation method of the rheum emodin double-chain biquaternary ammonium salt. The rheum emodin double-chain biquaternary ammonium salt is a mixture of a rheum emodin 1,3-site biquaternary ammonium salt and a rheum emodin 3,8-site biquaternary ammonium salt. The preparation method comprises the following steps: performing Williamson etherification reaction on rheum emodin and p-benzyl bromide in the presence of K2CO3 so as to generate a mixture of 1,3-site dibromomethylbenzyl rheum emodin and 3,8-site dibromomethylbenzyl rheum emodin, and further reacting the dibromomethylbenzyl rheum emodin mixture with tertiary amine so as to obtain the mixture of 1,3-site biquaternary ammonium salt and rheum emodin 3,8-site biquaternary ammonium salt, wherein the two biquaternary ammonium salts are isomerides which cannot be separated on a chromatographic column. The anti-cancer activity evaluation shows that the activity of the rheum emodin double-chain biquaternary ammonium salt disclosed by the invention is higher than that of monoquaternary ammonium salt, and the rheum emodin double-chain biquaternary ammonium salt can be used in a medicine for treating malignant tumor, is particularly applicable to treatment on liver cancer, has a relatively small inhibition effect on normal cells, and has relatively great application prospect.

The invention provides application of an emodin anthraquinone derivative in preparation of anti-hepatocellular carcinoma drugs. The emodin anthraquinone derivative is a 1, 4-dimethyl-6, 8-dimethoxy-9, 10-anthraquinone. The derivative not only can well inhibit proliferation of hepatocellular carcinoma cells, induce their apoptosis, and also has very small toxicity to normal cells. The derivative can be used in conjunction with 5-FU, cis-platinum and other chemotherapy drugs to increase the sensitivity of the chemotherapy drugs, thus achieving the effect of inhibiting hepatocellular carcinoma cell growth. Therefore, the emodin anthraquinone derivative can be used for preparing anti-hepatocellular carcinoma drugs or adjuvant chemotherapy drugs.

A quality test method for the Chinese medicine used to treat ophthalmopathy, myopia and eye strain features that a qualitative discrimination is used for paeonol, chrysophanol, emodin, ararobinol, paeoniflorin, danshensu sodium and icariin, and a high-effect liquid-phase chlomatography is used to test the content of berberine hydrochloride.

The invention discloses application of emodin derivatives 3-acetic emodin and 1,8-dihexanoyl emodin in preparation of anti-HIV-1 medicine, and belongs to the field of anti-virus medicines. 3-acetic emodin and 1,8-dihexanoyl emodin are prepared by means of chemical synthesis, the effect of inhibiting replication of HIV-1 viruses in vitro of 3-acetic emodin and 1,8-dihexanoyl emodin is discovered, the number of virus inclusion bodies in infected human macrophages is remarkably reduced, and the 3-acetic emodin and 1,8-dihexanoyl emodin do not have toxic or side effect on the human macrophages. Along with the increasing concentration of the 3-acetic emodin and 1,8-dihexanoyl emodin, the inhibition effect of the 3-acetic emodin and 1,8-dihexanoyl emodin on replication of HIV-1 in vitro is enhanced, expression of Gag genes and p24 protein is gradually reduced; meanwhile, the longer drugs acts, the more remarkable the effect of inhibiting HIV-1 infection is; and the 3-acetic emodin and 1,8-dihexanoyl emodin can be used for preparing anti-HIV-1 medicines.

The invention discloses a method for extracting bioactive ingredients in rhizoma polygoni cuspidati. The method includes the following steps that rhizoma polygoni cuspidati is taken, ground, screened with a 18-22-mesh sieve and put into a reflux tank; 75-85% ethyl alcohol with the weight 6-10 times that of rhizoma polygoni cuspidati is added, and reflux is carried out for 2.5-3.5 h at 55-65 DEG C; saturated ammonium sulfate is added into reflux liquid till precipitate is generated, and filtering is carried out to remove precipitate; ethyl alcohol is recycled, and alcohol extraction liquid is obtained; concentration is carried out, and concentrated extract is obtained; water precipitation is carried out, and supernatant liquid is taken; concentration is carried out again, and concentrated extract is obtained; alcohol dissolution is carried out, and supernatant liquid is taken; the supernatant liquid is subjected to plate ultrafiltration with SPES, and precipitate and supernatant liquid are collected; separation and purification of rheum emodin are carried out, wherein the purity of rheum emodin is 99.8%; the supernatant liquid is adsorbed with macroporous resin; separation and purification of polydatin are carried out, wherein the purity of polydatin is 99.5% or above; separation and purification of resveratrol are carried out, wherein the purity of resveratrol is 99.2% or above. According to the method, adopted equipment is simple, the process is simple and feasible, and industrial production is convenient; an adopted solvent is free of toxins or residues and does not cause environmental pollution.

The invention provides an osteogenic induction method of periodontal ligament stem cells (PDLSCs), which comprises the following steps: by using a third molar or premolar of a healthy patient as a raw material, separating and culturing the PDLSCs; and inoculating the PDLSCs in an osteogenic induction differentiation culture medium with the inoculum size of 2.0*10<4> / cm<2>, putting in a 37-DEG C cell culture box, and carrying out induction differentiation for 2 weeks, wherein the osteogenic induction differentiation culture medium is prepared by adding 10<-3> mol / L icariin, 10<-6> mol / L emodin, 10<-5> mol / L puerarin, 10<-6> mol / L berberine, 10<-5> mol / L encommiol extract, 2 mg / L drynaria total flavone, 4 mg / ml psoralen, 4 mg / ml oleanolic acid and 10<-6> mol / L Chinese teasel root saponin VI into a basal culture medium. The method can successfully implement osteogenic induction of the PDLSCs without influencing the stem cell characteristics and immunologic characteristics of the PDLSCs.

The invention discloses a method for simultaneous determination of six active components consisting of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride in a Niuhuang Ninggong tablet through HPLC. Chromatographic conditions employed in the invention are as follows: a chromatographic column is TC-C18 (4.6 mm * 250 mm, 5 [mu]m); detection wavelength is 280 nm; a mobile phase is methanol-0.05% phosphoric acid; gradient elution comprises three parts, i.e., elution with methanol with a concentration varying in a range of 10 to 80% in the time period from 0 min to 35 min, then elution with methanol with a concentration of 80% in the time period from 35 to 50 min, and finally elution with methanol with a concentration varying in a range of 80 to 10% in the time period from 50 to 60 min; flow velocity is 1.0 mL / min; column temperature is 25 DEG C; and sample size is 10 [mu]L. Under the above-mentioned chromatographic conditions, chromatographic peaks are perfectly separated, and concentrations and peak areas of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride show good linear relation. The method is simple, rapid and accurate, has good repeatability and can provide quality bases for comprehensive evaluation and control of the Niuhuang Ninggong tablet.

The invention relates to a method for measuring effective ingredients of shenshuanning tablets. The method includes the following steps of 1, preparing reference substance solutions, wherein proper quantities of reference substances including salvianic acid A sodium, salvianolic acid B, hesperidin, berberine hydrochloride, aloe-emodin, rheum emodin, rheinic acid, chrysophanol and emodin monomethyl ether are taken and precisely weighed, and methyl alcohol is added to prepare solutions; 2, preparing reference medicinal material solutions, wherein radix pseudostellariae powder, coptidis rhizome powder, rhizoma pinelliae powder, dried tangerine powder, poria cocos powder, rheum officinale powder, powder of the root of red-rooted salvia, powder of the root of bidentate achyranthes, safflower powder and licorice root powder are taken and precisely weighed, and methyl alcohol is added to prepare solutions; 3, preparing a solution for a test product, wherein sample powder is taken and precisely weighed, and methyl alcohol is added to prepare the solution; 4, carrying out a measuring method, wherein 5 microliters to 15 microliters of the solutions are respectively taken and respectively injected into a liquid chromatograph for testing, and chromatograms are recorded and compared with standard fingerprints.

The invention discloses an in-situ adipocyte composite inhibitor for the local weight loss and body care of human bodies and a preparation method thereof. The compound (composite inhibitor) comprises the following bulk drugs: anti-NPY monoclonal antibodies, alpha-aminoethanesulfonic acid, mannitol, enol phosphatidylcholine, sodium deoxycholate, trans-10, cis-12conjugated linoleic acid, docosahexenoic acid, berberine, emodin, sodium bicarbonate and injection water. The compound of the invention can synchronously inhibit the propagation and the differentiation of preadipocytes, accelerate and promote the decomposition and the conversion of fats in mature adipocytes, effectively reduce the quantity of in-situ and newborn mature adipocytes, regulate the expression, the synthesis and the secretion of relevant adipogenic genes and adipocyte growth factors from the gene level and the like, and control the propagation and the fat synthesis of the adipocytes at the source; and, additionally, the absorption utilization rate of the compound is improved through local administration, so the compound has the rapid and efficient weight loss effect.