39 results about "Emodin" patented technology

The invention relates to the technical field of veterinary drugs, and particularly provides a veterinary carprofen injection and a preparation method thereof, the veterinary carprofen injection comprises carprofen, emodin, galuteolin, coumarin, Radix Astragali, wolfberry, Schisandra chinensis, resveratrol and other components. According to the veterinary carprofen injection, emodin and luteoloside are added for compound anti-inflammation, the dosage of carprofen is reduced, the kidney burden is relieved, meanwhile, drug resistance is not likely to be generated, radix astragali, fructus lycii and fructus schizandrae extracts are added for protecting the kidney, regulating immunity and avoiding renal function damage, and finally the effects of compound anti-inflammation and renal function damage avoiding are achieved.

The invention relates to the technical field of pharmaceutical analysis, in particular to an HPLC (High Performance Liquid Chromatography) fingerprint spectrum construction method and application of a twelve-ingredient numbness pain liniment. The construction method comprises the following steps: preparing a test solution; preparing a reference substance solution; and HPLC determination. The HPLC fingerprint spectrum construction method of the twelve-ingredient numbness pain liniment is provided for the first time, and the method has the advantages of being high in precision, good in reproducibility, high in stability and good in durability. Meanwhile, the method disclosed by the invention can be used for detecting the contents of three components including polydatin, stilbene glucoside and emodin in the twelve-ingredient numbness pain liniment at one time. Technical support is provided for comprehensively improving the product quality standard of the twelve-ingredient arthralgia pain liniment and optimizing the preparation process, identifying true and false and carrying out follow-up material basic research of the twelve-ingredient arthralgia pain liniment.

The invention belongs to the technical field of pest control, and particularly relates to a method for efficiently controlling guava leaf rollers. The invention relates to an insecticidal composition. The insecticidal composition is prepared by binary compounding of an effective component I and an effective component II, the effective component I is pyridalyl, and the effective component II is emodin; the mass ratio of the effective component I to the effective component II is (1-100): (100-1). According to the prevention and control method, the insecticidal composition is selected for foliage spraying, meanwhile, the guava fruits are subjected to bagging treatment, and therefore the angutortria can be effectively prevented and controlled. When the effective components in the insecticidal composition are compounded, the insecticidal composition has a synergistic effect on psidium guajava leaf roller, and compared with a single effective component, the insecticidal composition can improve the prevention and control effect on the psidium guajava leaf roller, reduce the use dosage of pesticides and reduce the influence on the environment; in addition, generation and development of pesticide resistance can be delayed, the service life of the pesticide is prolonged, and comprehensive prevention and control of agricultural pests are facilitated.

The application discloses a composite photocatalytic bacteriostatic agent and a preparation method thereof, and belongs to the technical field of aquatic diseases. The emodin (Em) is loaded on sodium alginate (SA) to prepare the composite photocatalytic bacteriostatic agent Em / SA. Compared with the emodin alone, the composite photocatalytic bacteriostatic agent Em / SA can further improve the inactivation effect on Aeromonas hydrophila cells. The survival rate of the Aeromonas hydrophila with a bacterial concentration of 10 7 cfu / mL is reduced from 99.33% when the emodin is used alone to less than 21.65%. The preparation method of the photocatalytic bacteriostatic agent Em / SA is simple and green, the composite photocatalytic bacteriostatic agent Em / SA only contains the natural product emodin and sodium alginate, the reaction is carried out under light, no secondary pollution is caused to the environment, and the photoinactivation of the Aeromonas hydrophila can be efficiently realized under white light illumination for 30 min.

This invention provides a fingerprint spectrum of Yishen Wufa Oral Liquid, its construction method, and its application, as well as a multi-index component detection method for Yishen Wufa Oral Liquid, relating to the field of pharmaceutical analysis and structural technology. The fingerprint spectrum of Yishen Wufa Oral Liquid constructed by this invention includes 20 characteristic peaks, including ferulic acid, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, astragaloside A, psoralen, isopsoralen, and emodin. The chromatographic peaks are abundant and attributed to the effective components of psoralen (roasted with black sesame seeds), angelica, poria cocos, wolfberry, fleeceflower root (processed with black bean wine), achyranthes bidentata, and astragalus complanatus. This provides a more comprehensive reflection of the overall chemical composition and quality of Yishen Wufa Oral Liquid, facilitating more effective guidance for feed addition and strict standardization of production operations. It also helps improve the safety and efficacy of Yishen Wufa Oral Liquid in clinical use, possessing high application value.

The present application relates to a kind of methyltransferase that can catalyze anthraquinone compound methylation, amino acid sequence is as shown in SEQ ID NO:2, also provide the application of this methyltransferase in the production of emodin-3-methyl ether and other methylated anthraquinone compounds.The present application digs a new methyltransferase, which can methylate anthraquinone compound, and can convert emodin into emodin-3-methyl ether.The present application constructs the engineering bacteria that can produce emodin-3-methyl ether using the coding gene of the enzyme, widens the methyltransferase library that can be used for emodin methylation, provides new way for emodin-3-methyl ether cell factory construction.

This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing benzimidazole. The pesticide composition's active ingredient is a compound of benzimidazole and fluazinam or emodin methyl ether. This invention, through indoor bioactivity tests, found that the compound of benzimidazole with fluazinam or emodin methyl ether, within a certain mass ratio range, has a synergistic effect against various plant fungal diseases such as Rhizoctonia solani, Curvularia crescentis, and Sclerotium sclerotiorum, helping to improve control efficacy. This is of great significance for the research and development of pesticides for controlling diseases such as cucumber damping-off, corn curvularia leaf spot, and peanut white mold.

This invention discloses an emodin-modified amine curing agent and its preparation method, as well as a long-lasting anti-corrosion coating based on bulk rust conversion, its preparation method, and its application. It relates to the field of anti-corrosion coating technology. The preparation method of the emodin-modified amine curing agent of this invention includes: subjecting raw materials including 2-hydroxyemodin, formaldehyde, and 1,5-pentanediamine to a Mannich reaction to obtain the emodin-modified amine curing agent. The emodin-modified amine curing agent used in this invention has a certain rust conversion ability and forms a stable chelate with iron ions, eliminating the need for rust conversion agents and allowing direct application to corroded surfaces. By using a suitable resin and curing agent system ratio, this invention can prepare a high-performance, low-surface-treatment, long-lasting anti-corrosion coating based on bulk rust conversion, applicable to manually cleaned surfaces (St2 / St3, ISO 8501-1), old coatings, high-pressure water jetting, and wet sandblasting substrate surfaces.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

The invention belongs to the field of biological information analysis and enzyme engineering, and particularly relates to application of methyltransferase in converting emodin into emodin monomethyl ether. The invention discloses an application of methyltransferase in converting emodin into physcion. The amino acid sequence of the methyltransferase comprises an amino acid sequence as shown in SEQ ID NO: 1-5 (particularly the methyltransferase as shown in SEQ ID NO: 1). The invention also provides a recombinant microorganism expressing the methyltransferase.

The application relates to the field of biological medicine, and discloses a protein-based co-crystal gel as well as a preparation method and application thereof. The protein-based co-crystal gel comprises gelatin, an antibacterial component and a deep eutectic solvent, wherein the components of the Gel-COS-Ed co-crystal gel comprise gelatin, chitosan oligosaccharide, emodin, glycerol and betaine. After the gelatin and the antibacterial component are heated and dissolved, the gelatin is coagulated into a gelatin gel; the gelatin gel is placed in the deep eutectic solvent to obtain the protein-based co-crystal gel. The tensile strength of the obtained Gel-COS-Ed co-crystal gel is 1.01 MPa, the breaking strain can reach 510%, there is no obvious phase change in the temperature range of-80 DEG C to 80 DEG C, the antibacterial rate on staphylococcus aureus reaches 95.7%, and a new solution is provided for the application of wound dressings and biological medical materials.

This invention discloses a method for preparing TCPP / Emodin composite photocatalysts and their antibacterial applications, belonging to the field of pharmaceutical antibacterial medicine. Addressing the issues of drug resistance easily induced by traditional antibiotics in treating bacterial infections, performance defects in existing photocatalysts, and insufficient antibacterial capacity of single antibacterial materials, this invention leverages the advantages of tetracarboxyphenylporphyrin (TCPP)—strong visible light capture ability and low photogenerated electron-hole recombination rate—and complements emodin's π-conjugated system and its structural advantage of promoting carrier migration. The two are covalently linked through a chemical coupling mechanism involving EDC / NHS synergistic activation of the carboxyl group. Through steps such as dissolution, mixing reaction, dialysis purification, and freeze-drying, spherical TCPP / Emodin composite nanomaterials with a particle size of 100-500 nm are obtained. This composite material reduces the photogenerated electron-hole recombination rate and improves carrier migration efficiency, efficiently generating ROS under visible light irradiation, and exhibits excellent photodynamic antibacterial properties.

This invention belongs to the field of nanomedicine delivery systems, specifically relating to a dual-sensitivity co-loaded emodin and chlorambucil micelles, their preparation method, and applications. The polymer structure is as follows: Using polymethacrylate as the linking unit, this invention designs and synthesizes a dendritic polymer p(mPEG-co-HPBE-co-EMD) grafted with polyethylene glycol monomethyl ether, p-hydroxymethylphenylboronic acid pinacol ester, and emodin, and uses it for encapsulating chlorambucil. This co-loaded polymer micelle can be used for the co-delivery of emodin and chlorambucil. This co-loaded micelle improves the water solubility of the drug, has a suitable particle size, and dual ROS / pH sensitivity, enabling targeted release of emodin and chlorambucil at tumor sites, achieving synergistic treatment with chemotherapy and oxidative therapy. In the formula, n = 30-85, x:y:z = 1:2-5:1-3.

The invention discloses application of emodin in preparation of a medicine for treating cow foot rot, HE staining and transmission electron microscopy discover that emodin can effectively relieve damage of necrobacter to cow interdigital skin explants, and a new candidate medicine is provided for clinical treatment of cow foot rot.

The invention discloses an application of Danjie emodin in preparation of a product for inhibiting T cell depletion. It is found through in-vitro screening that the Danjie emodin can significantly inhibit T cell depletion. Mechanism research shows that the Danjie emodin does not influence the secretion of Granzyme B and slightly inhibits the secretion level of a cell factor IFN-gamma; by inhibiting an AKT phosphorylation signal channel, accumulation of protein aggregates in T cells is remarkably reduced, and the cell protein homeostasis is maintained, so that T cell depletion is inhibited. Animal experiments also prove that the T cells treated by the Danjie emodin can significantly inhibit the growth of melanoma after adoptive reinfusion. Therefore, the invention provides the application of the Danjie emodin in preparation of the product for inhibiting T cell depletion, and further provides a new small molecule metabolism intervention strategy for improving the solid tumor immunotherapy effect.

The present application belongs to the technical field of pesticides, and particularly relates to a biological pesticide for preventing and treating citrus red mite. The biological pesticide is prepared by compounding emodin with abamectin or osthole. When emodin is combined with abamectin or osthole, a synergistic effect is exhibited on the citrus red mite, the prevention and treatment effect on the citrus red mite can be improved, the problems caused by long-term use of the same type of pesticide or the same pesticide can be alleviated, the generation and development of the drug resistance of the citrus red mite can be delayed, and support is provided for the comprehensive management of the drug resistance. In addition, emodin, abamectin and osthole are all biological pesticide components, are environment-friendly and green, and can provide support for developing the biological pesticide for preventing and treating the citrus red mite.

The application discloses a composition for repairing meniscus, which comprises a growth differentiation factor and emodin, improves inflammation and oxidative stress in a microenvironment, and promotes cell chondrogenic differentiation process. It is verified that the "core-shell" co-delivery nano carrier provided by the application uses mesoporous silica nanoparticles to wrap two bioactive compounds, emodin and a growth differentiation factor, and the latter is used for promoting fibrocartilage differentiation. The dual-drug nano delivery system provides sustained release of emodin and GDF, synergistically regulates a harsh inflammatory and oxidative environment, and promotes fibrocartilage regeneration in vitro and in vivo. Benefited from this, the application also combines a three-dimensionally printed meniscus-shaped PCL frame with the composition by using a cartilage-specific matrix hydrogel, constructs a reinforced stability meniscus replacement model, has a synergistic regulation on a cartilage immune microenvironment, and realizes successful repair of a whole meniscus replacement operation of an animal (rabbit).

The application discloses a method for establishing a Qingre Mingmu tea fingerprint spectrum by using a high performance liquid chromatography method and application. By using the method, 17 fingerprint peaks can be confirmed, and three components of fried cassia seed, chrysanthemum and sweetleaf chrysanthemum in the Qingre Mingmu tea have corresponding attribution peaks. By comparison with reference substances and in combination with ultraviolet absorption spectrum characteristics, characteristic peaks of six chemical components, i.e., chlorogenic acid, rebaudioside A, stevioside, citranaxanthin, emodin and chrysophanol, are identified, wherein the rebaudioside A and the stevioside are characteristic peaks of the sweetleaf chrysanthemum, and the two characteristic peaks can distinguish the chrysanthemum and the sweetleaf chrysanthemum. The fingerprint spectrum detection method disclosed by the application can obtain a fingerprint spectrum with good separation degree and comprehensive chromatographic peaks, can more objectively and comprehensively evaluate the overall quality of the Qingre Mingmu tea, and lays a foundation for quality control of the Qingre Mingmu tea.

The invention belongs to the technical field of traditional Chinese medicine detection and analysis, and discloses a traditional Chinese medicine fingerprint spectrum detection method of lipid-lowering granules. 2, 3, 5, 4 '-tetrahydroxystilbene-2-O-beta-D glucoside, aurantio-obtusin, emodin, physcion and ursolic acid are taken as reference substances, a high performance liquid chromatograph is adopted for detection, a traditional Chinese medicine fingerprint spectrum similarity evaluation system analyzes chromatograms of all the samples, and a reference fingerprint spectrum is generated; and S3, calculating the similarity between the to-be-detected sample and the control fingerprint spectrum in a traditional Chinese medicine fingerprint spectrum similarity evaluation system according to the chromatogram obtained in the step S3, and when the similarity is not lower than 0.95, judging that the to-be-detected sample is a qualified lipid-lowering preparation. The method provided by the invention can comprehensively and accurately evaluate the overall quality of the lipid-lowering granules. The detection method is simple, good in reproducibility and stability and high in detection accuracy.

The application discloses a heart-protecting granule identification and quality control method based on a thin layer chromatography, and belongs to the technical field of drug detection. The thin layer chromatography identification method comprises the following steps: taking astragaloside as a control product to identify astragalus membranaceus, taking chrysophanol and emodin as control products to identify largeleaf rhubarb, taking hirudo medicinal materials as a control product to identify hirudo, taking glycyrrhizin and licorice medicinal materials as control products to identify licorice, taking catalpol as a control product to identify radix rehmanniae, taking salvianolic acid B and salvia miltiorrhiza medicinal materials as control products to identify salvia miltiorrhiza, and taking naringin and neohesperidin as control products to identify aurantii fructus. The application establishes the thin layer chromatography identification method of the heart-protecting granule, adopts a special developing agent and a color developing mode, realizes accurate identification according to the spot color, fluorescence characteristics and other characteristics of the corresponding positions of the test product chromatogram, the control product and the control medicinal material chromatogram, the method is simple in operation, low in detection cost, strong in repeatability and specificity, and can effectively control the quality of the heart-protecting granule production process.

The invention belongs to the technical field of pharmaceutical compositions, and particularly relates to an aloin composition for inhibiting growth of desulfurization vibrio and generation of hydrogen sulfide as well as a screening method and application of the aloin composition. The barbaloin, the flavone, the hippophae rhamnoides extract, the sappan wood extract and the erigeron breviscapus extract are uniformly mixed to prepare a raw material mixture, and the obtained composition is light yellow powder and has good effects of inhibiting growth of sulfate reducing bacteria and desulfurization vibrio and reducing generation of hydrogen sulfide; the method is especially suitable for solving the problem of hydrogen sulfide emission caused by sulfate reducing bacteria such as desulfurization vibrio. The method for inhibiting growth of sulfate reducing bacteria and desulfurization vibrio and reducing generation of hydrogen sulfide into natural compounds through virtual screening is novel, the composition preparation method is easy to operate, raw materials are easy to obtain, cost is low, repetition is easy, and the method has important significance on preparation of products for inhibiting growth of desulfurization vibrio and generation of hydrogen sulfide.

The application relates to the technical field of kidney treatment, and particularly discloses a method for extracting emodin and application of the emodin in renal ischemia-reperfusion injury. The method comprises the following steps: S1, crushing traditional Chinese medicine rhubarb raw materials to obtain traditional Chinese medicine rhubarb powder; S2, mixing the traditional Chinese medicine rhubarb powder with ethanol and performing ultrasonic treatment to obtain a primary extraction solution; S3, adding cellulase to the primary extraction solution to obtain an enzymatic solution; S4, performing supercritical CO2 extraction on the enzymatic solution, and collecting an extraction phase; S5, adding ethyl acetate to the extraction phase for extraction, and then separating an organic phase; S6, crystallizing the organic phase, and filtering to obtain emodin crude products; and S7, recrystallizing the emodin crude products with methanol, and drying to obtain finished products. The method for extracting emodin can be used for preparing medicines for treating kidney diseases, in particular, renal ischemia-reperfusion injury, and has the advantages of high extraction efficiency, high product purity and a green and environment-friendly process.

This invention proposes a method for establishing a high-performance liquid chromatography (HPLC) fingerprint spectrum of a traditional Chinese medicine compound preparation and its application. The method includes: (1) taking an appropriate amount of the traditional Chinese medicine compound preparation, adding a solvent, and sonicating it to obtain a test solution; (2) performing HPLC detection on the test solution obtained in step (1). The detection conditions are: using a C18 column; using acetonitrile as mobile phase A and 0.15% phosphoric acid aqueous solution as mobile phase B for gradient elution; flow rate 0.8-1.2 mL / min, column temperature 28℃-35℃, injection volume 8-12 μL, and detection wavelength 290-300 nm. The HPLC fingerprint spectrum determined by the method described in this application can rapidly and effectively identify six components: chlorogenic acid, cryptochlorogenic acid, polygalactoside, strychnoside, emodin-8-O-β-D-glucoside, and emodin, and has the advantages of stability and high precision.

The present invention provides a preparation method of a Fuyanxiao oral solid preparation, belonging to the field of pharmaceutical technology. The Fuyanxiao oral solid preparation is made from seven medicinal materials including Oxalis corniculata, Patrinia scabiosaefolia, Trichosanthes kirilowii Maximowicz, Rheum officinale, Paeonia suffruticosa, Atractylodes lancea, and Lindera aggregata. The preparation method is to coarsely crush 1 / 2 weight portion of the Rheum officinale medicinal material with a 4-cm diameter sieve plate into coarse granules with a particle size of 4 cm, conduct moist heat sterilization and drying, and then superfine pulverize it into nanoscale fine powder for standby. Then, take the remaining Rheum officinale medicinal material and the other medicinal materials to extract and prepare dry extract powder for standby. Finally, select appropriate excipients according to the oral solid preparation to be prepared and mix them to make the corresponding dosage form. Compared with the prior art, the present invention solves the problem of unstable emodin content by changing the addition method of raw Rheum officinale powder, and at the same time provides preparation methods for various oral solid preparations, providing more medication options for patients.

The invention belongs to the technical field of biology, and discloses an application of emodin-8-glucoside in resisting tembusu virus. An in-vitro experiment is carried out by using a recombinant duck tembusu virus stably expressing EGFP (Enhanced Green Fluorescent Protein), the emodin-8-glucoside is proved to be capable of efficiently inhibiting virus replication, the median effective concentration (EC50) of the emodin-8-glucoside is as low as 1.935 mu M, the median cytotoxic concentration (CC50) of the emodin-8-glucoside is as high as 511.3 mu M, and the calculated therapeutic index (SI) is 264.24, so that the compound has the outstanding characteristics of high efficiency and low toxicity, and can be used for preparing a medicine for preventing and treating the virus replication. The clinical application safety is high; duck infection model experiments prove that the emodin-8-glucoside can effectively inhibit the duplication of DTMUV in duck bodies, and has a definite treatment effect; meanwhile, the invention also provides a medicine, a feed and a feed additive containing the emodin-8-glucoside.

The present application relates to a kind of regulating gastrointestinal function of the composition of food and medicine and its preparation method and application.The present application uses the medicinal material of food and medicine, such as Cistanche, Cassia seed, Codonopsis, hawthorn, immature fruit of Citrus aurantium, angelica and licorice, reasonable compatibility as main effective component, further can increase seasoning, functional auxiliary material black sesame, walnut, Chinese yam, jujube, dietary fiber or honey, and be made into suitable product of taste.The efficacy application result verifies that the composition has good spleen and kidney, blood and qi, moistening intestine and defecation effect, can significantly regulate gastrointestinal function, improve the treatment effect on acute constipation and chronic constipation;After increasing functional auxiliary material, not only have the effect of preparation forming and improving taste, but also have the function of significantly improving intestinal peristalsis, secretory water, reducing the adverse effects of long-term use of emodin-containing traditional Chinese medicine, etc.The regulating gastrointestinal function of the composition of food and medicine of the present application is suitable for long-term constipation population, especially suitable for weak old people.

The invention discloses a preparation method and application of an emodin derivative with an effect of relieving hepatic fibrosis, and belongs to the technical field of medicine synthesis. According to the technical scheme, the emodin derivative molecule has the structure, wherein R1 is a hydrogen atom or R is hydroxyl or X is a phenyl derivative or a benzyl derivative. The invention designs and synthesizes an emodin derivative with a novel structure, and the compound can regulate and control copper ions in a targeted manner so as to have an effect of relieving hepatic fibrosis.

This invention belongs to the field of agricultural biological control technology, specifically relating to *Trichoderma harzianum* IVF1 for controlling fungal diseases and its application. The *Trichoderma harzianum* (… Trichoderma harzianum The preservation number of this invention is CGMCC No. 42324. The *Trichoderma harzianum* strain of this invention can effectively inhibit gray mold in cucumbers and wilt disease in cowpeas. Under inactivated gray mold induction conditions, the antibacterial active substances produced by *Trichoderma harzianum* of this invention are increased, further enhancing the control efficacy. Transcriptome results show that differentially expressed genes are significantly enriched in pathways related to secondary metabolite biosynthesis, antibiotic synthesis, and amino acid synthesis. Metabolome results show that the production of important biologically active organic acids such as citric acid, benzene ring compound emodin, organic heterocyclic compound 6-methylnicotinic acid, and *Trichoderma diol* is significantly increased.

The present application provides a kind of content determination method of Fuyanxiao preparation, belong to the technical field of drug detection, the Fuyanxiao preparation is made of seven medicinal materials such as Oxalis corniculata L., Herba Patriniae, Radix Trichosanthis, Radix et Rhizoma Rhei, Cortex Moutan, Atractylodes, Linder, etc., the content determination method is using high performance liquid chromatography method same chromatographic condition is carried out content determination to 14 kinds of components in product, such as gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, emodin, chrysophanol, physcion.The present application increases the content determination of gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, chrysophanol, physcion compared with prior art, improves the requirement of product quality detection, is favorable for guaranteeing drug quality and clinical efficacy.