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2283 results about "Gel electrophoresis" patented technology

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.

Recombinase polymerase amplification

This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.
Owner:ABBOTT DIAGNOSTICS SCARBOROUGH INC

Truncated fragments of alpha-synuclein in Lewy body disease

ActiveUS20050198694A1Useful pharmacological activityBiocideNervous disorderGel electrophoresisC-terminus
The application identifies novel fragments of alpha-synuclein in patients with Lewy Body Disease (LBD) and transgenic animal models thereof. These diseases are characterized by aggregations of alpha-synuclein. The fragments have a truncated C-terminus relative to fill-length alpha-synuclein. Some fragments are characterized by a molecular weight of about 12 kDa as determined by SDS gel electrophoresis in tricine buffer and a truncation of at least ten contiguous amino acids from the C-terminus of natural alpha-synuclein. The site of cleavage preferably occurs after residue 117 and before residue 126 of natural alpha-synuclein. The identification of these novel fragments of alpha-synuclein has a number of application in for example, drug discovery, diagnostics, therapeutics, and transgenic animals.
Owner:TRUSTEES OF FLINDERS UNIV +2

Process for analyzing protein samples

Methods using gel electrophoresis and mass spectrometry for the rapid, quantitative analysis of proteins or protein function in mixtures of proteins derived from two or more samples in one unit operation are disclosed. In one embodiment the method includes (a) preparing an extract of proteins from each of at least two different samples; (b) providing a set of substantially chemically identical and differentially isotopically labeled protein reagents; (c) reacting the extract of proteins from different samples of step (a) with a different isotopically labeled reagent from the set of step (b) to provide two or more sets of isotopically differentially labeled proteins; (d) mixing each of the two or more sets of isotopically labeled proteins to form a single mixture of isotopically differentially labeled proteins; (e) electrophoresing the mixture of step (d) by an electrophoresing method capable of separating proteins within the mixture; (f) digesting at least a portion of one or more separated proteins of step (e) and (g) detecting the difference in the expression levels of the proteins in the two samples by mass spectrometry based on one or more peptides in the sample of labeled peptides. The analytical method can be used for qualitative and particularly for quantitative analysis of global protein expression profiles in cells and tissues, i.e. the quantitative analysis of proteomes.
Owner:DH TECH DEVMENT PTE +1

Aptamer-based methods for identifying cellular biomarkers

In this invention, a biomarker discovery method has been developed using specific biotin-labeled oligonucleotide ligands and magnetic streptavidin beads. In one embodiment, the oligonucleotide ligands are firstly generated by whole-cell based SELEX technique. Such ligands can recognize target cells with high affinity and specificity and can distinguish cells that are closely related to target cells even in patient samples. The targets of these oligonucleotide ligands are significant biomarkers for certain cells. These important biomarkers can be captured by forming complexes with biotin-labeled oligonucleotide ligands and collecting the complexes using magnetic streptavidin beads, whereupon the captured biomarkers are analyzed to identify the biomarkers. Analysis of biomarkers include HPLC-Mass Spectroscopy analysis, polyacrylamide gel electrophoresis, flow cytometry, and the like. The identified biomarkers can be used for pathological diagnosis and therapeutic applications. Using the disclosed methods, highly specific biomarkers of any kinds of cells, in particular cancer cells, can easily be identified without prior knowledge of the existence of such biomarkers.
Owner:TAN WEIHONG +1

Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

InactiveUS6881317B2Rapid fractionationSludge treatmentVolume/mass flow measurementDNA SolutionsPulsed field electrophoresis
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
Owner:THE TRUSTEES FOR PRINCETON UNIV

Recombinase polymerase amplification

This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.
Owner:ABBOTT DIAGNOSTICS SCARBOROUGH INC

Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis

The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
Owner:ADVANCED LIQUID LOGIC +1

Truncated fragments of alpha-synuclein in Lewy Body Disease

The application identifies novel fragments of alpha-synuclein in patients with Lewy Body Disease (LBD) and transgenic animal models thereof. These diseases are characterized by aggregations of alpha-synuclein. The fragments have a truncated C-terminus relative to full-length alpha-synuclein. Some fragments are characterized by a molecular weight of about 12 kDa as determined by SDS gel electrophoresis in tricine buffer and a truncation of at least ten contiguous amino acids from the C-terminus of natural alpha-synuclein. The site of cleavage preferably occurs after residue 117 and before residue 126 of natural alpha-synuclein. The identification of these novel fragments of alpha-synuclein has a number of application in for example, drug discovery, diagnostics, therapeutics, and transgenic animals.
Owner:PROTHENA BIOSCI LTD

Dispersion of carbon nanotubes by nucleic acids

Carbon nanotubes dispersed by a stabilized solution of nucleic acid molecules formed separated nanotube-nucleic acid complexes and were separated according to standard chromatographic methods, including gel electrophoresis, two phase solvent systems and ion exchange chromatography.
Owner:EI DU PONT DE NEMOURS & CO

Gel electrophoresis training aid and training kit

A gel electrophoresis training aid is provided. The training aid includes a substantially transparent elastomeric body having at least one series of spaced-apart wells. The elastomeric body is adapted with a plurality of spaced-apart bands extending in the same direction from the wells. Each of the spaced-apart bands are positioned substantially parallel to one spaced-apart well. A training kit including the training aid is also provided.
Owner:VON ENTERPRISES

Method for extracting keratin from feather

The invention provides a method for extracting keratin from feather, comprising the steps that after being crashed, clean feather is pretreated by ethanol and hydrochloric acid; by adopting mercaptoethanol reduction method, the pretreated feather is deacidized to obtain keratin solution of the feather, and then clean sand white keratin solid powder is obtained through acid coprecipitate, washing and drying. The extracted keratin is analyzed and represented by the methods such as ultraviolet-visible spectrum, infrared spectrum, SDS-gel electrophoresis, elementary analysis, etc. The results shows that the keratin extracted by the method mainly contains the elements such as C, H, N, O, S, the total content of which reaches over 99%, and the purity is higher; the molecular weight of the keratin mainly ranges from 5 to 20 kD and 40 to 80kD.
Owner:NORTHWEST NORMAL UNIVERSITY

Method for treating systemic bacterial, fungal and protozoan infection

The invention is directed to a treatment of diseases that are accompanied by quantitative and / or qualitative changes of blood extracellular DNA and, more particularly, to a treatment of systemic bacterial, fungal and protozoan infections. The inventive method comprises introducing a treatment agent into a circulating blood system of a patient diagnosed with systemic infection caused by bacteria, fungi or protozoa, wherein said treatment agent destroys extracellular DNA in said blood of said patient and wherein said treatment agent used to destroy said extracellular DNA is a DNase enzyme: said agent being administered in doses and regimens which are sufficient to decrease the average molecular weight of circulating extracellular blood DNA in the blood of said patient; such decrease in the average molecular weight can be measured by gel electrophoresis of extracellular blood DNA fraction from the blood of said patient. A DNase enzyme may be applied in a dose and regimen that provide a DNase DNA hydrolytic activity measured in blood plasma that exceeds 1.5 Kunitz units per 1 ml of blood plasma for more than 12 hours within a period of 24 hours.
Owner:CLS THERAPEUTICS

Cell line gene knock-out method for obtaining large fragment deletion through CRISPR/Cas9 system

ActiveCN107435051AEasy accessImprove knockout productivityVectorsStable introduction of DNAFluorescenceLarge fragment
The invention relates to a cell line gene knock-out method for obtaining large fragment deletion through a CRISPR / Cas9 system, and belongs to the field of genetic engineering and genetic modification. A pX458 vector is modified, so that the pX458 vector is provided with DsRed2 and ECFP (Enhanced Cyan Fluorescence Protein); then, a plurality of specific sgRNA sites are designed by aiming at a target gene and are connected into the modified vector; after the cell line transfection, cell groups are sorted by a flow cytometry; single cells subjected to genome editing can be very fast obtained; then, a single-cell DNA (Deoxyribonucleic Acid) sequence is subjected to PCR (Polymerase Chain Reaction) amplification; and single cells with large fragment deletion can be picked out from the single cells through gel electrophoresis. The CRISPR / Cas9 system, the flow cytometry single-cell sorting and fluorescent protein screening on the expression vector are combined, so that the positive monoclonal cells with the large fragment deletion can be obtained in a short time; and the work efficiency of the cell line gene knock-out is greatly improved.
Owner:XINXIANG MEDICAL UNIV

Truncated fragments of alpha-synuclein in Lewy body disease

The application identifies novel fragments of alpha-synuclein in patients with Lewy Body Disease (LBD) and transgenic animal models thereof. These diseases are characterized by aggregations of alpha-synuclein. The fragments have a truncated C-terminus relative to full-length alpha-synuclein. Some fragments are characterized by a molecular weight of about 12 kDa as determined by SDS gel electrophoresis in tricine buffer and a truncation of at least ten contiguous amino acids from the C-terminus of natural alpha-synuclein. The site of cleavage preferably occurs after residue 117 and before residue 126 of natural alpha-synuclein. The identification of these novel fragments of alpha-synuclein has a number of application in for example, drug discovery, diagnostics, therapeutics, and transgenic animals.
Owner:PROTHENA BIOSCI LTD

Methods for the isolation and analysis of cellular protein content

The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization / Time of Flight (MALDI / TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.
Owner:UNITED STATES OF AMERICA

Method for treating systemic DNA mutation disease

A treatment for systemic DNA mutation diseases accompanied with development of somatic mosaicism and elevation of blood extracellular DNA and, more particularly, to a treatment of diabetes mellitus and atherosclerosis. The inventive method consist from introducing a treatment agent into a circulating blood system of a patient diagnosed with systemic DNA mutation diseases when said treatment agent destroys extracellular DNA in said blood of said patient and wherein said treatment agent used to destroy said extracellular DNA is a DNASE enzyme: said agent might be administered in doses and regimens which sufficient to decrease number average molecular weight of circulating extracellular blood DNA in the blood of said patient; such decrease of number average molecular weight might be measured by gel electrophoresis of extracellular blood DNA fraction from the blood of said patient. A DNASE enzyme may be further applied in a dose and regime that provide a DNA hydrolytic activity measured in blood plasma exceeding 1.5 Kunitz units per 1 ml of blood plasma for more than 12 hours within a period of 24 hours.
Owner:CLS THERAPEUTICS

Crosslinked swellable polymer

ActiveUS20100234252A1Stable and labile crosslinkersAdvantageously employedFluid removalFlushingFlocculationPapermaking
The invention is directed to stable crosslinked water-soluble swellable polymers, methods for making same, and their various uses in the hygiene and medical arts, gel electrophoresis, packaging, agriculture, the cable industry, information technology, in the food industry, papermaking, use as flocculation aids, and the like. More particularly, the invention relates to a composition comprising expandable polymeric microparticles having labile crosslinkers and stable crosslinkers, said microparticle mixed with a fluid and an unreacted tertiary crosslinker that is capable of further crosslinking the microparticle on degradation of the labile crosslinker so as to form a stable gel. A particularly important use is as an injection fluid in petroleum production, where the expandable polymeric particles are injected into a well and when the heat and / or pH of the well cause degradation of the labile crosslinker and when the particle expands, the tertiary crosslinker crosslinks the polymer to form a stable gel, thus diverting water to lower permeability regions and improving oil recovery.
Owner:CONOCOPHILLIPS CO

Environment DNA identification method for fish community structure researching

The invention relates to an environment DNA identification method for fish community structure researching. The method is characterized by comprising the steps that 1, water samples are collected according to the size of the water area where a fish community is located; 2, the water samples are processed, and total environment DNA of the processed water samples is extracted; 3, a 16s r DNA sequence with nucleotide sequences being SEQ ID NO:1 and SEQ ID NO:2 is adopted as a universal primer to carry out PCR amplification on the total environment DNA, and a PCR product is obtained; 4, gel electrophoresis is carried out on the PCR product, and gel with DNA is obtained; 5, gel cutting and clone sequencing are carried out on the gel with the DNA, and then blast comparison is carried out through GENBANK so as to determine whether the DNA is a fish sequence or not; 6, after it is determined that the DNA is the fish sequence, the next-generation sequencing technology is adopted for analyzing composition conditions of the PCR product on a large scale, and therefore the environment DNA fish composition and community structure are determined. The environment DNA identification method is quite easy, convenient and efficient and has the practical value.
Owner:SHANGHAI OCEAN UNIV

Identification method for animal derived materials

An identification method of animal source character component is provided, which relates to an identification method of food component. The invention overcomes that the current identification method of animal source character component has the defects of low testing sensitivity and unable to test the heat-processed manufactured meat. The animal source character component is identified by the following method: extracting the source character sample DNA of the animal for testing, PCR testing and gel electrophoresis, and then identifying the component of animal source character. The method in the invention has high testing sensitivity, and the identification number limit can be below 1 percent. The identification method in the invention can test the heat-processed manufactured meat, and mix manufactured meat and bone meal product. The identification number limit also can reach below 1 percent. The identification method in the invention doesn't use liquid nitrogen in identification process, which not only lowers the cost, but also improves the safety of the operation.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Gel electrophoresis dyeing device

The invention discloses a gel electrophoresis dyeing device, which is characterized by comprising a liquid storage device (1), a dyeing box (2) and a drive device (4), wherein the liquid storage device is used for storing a liquid reagent used in the dyeing process and is provided with a liquid pouring port and a liquid outlet port; the top of the dyeing box is sealed by a dyeing box cover plate (20); a liquid outlet port is formed on the dyeing box cover plate (20); a gel plate fixing device (3) used for fixing a plurality of gel plates is arranged in the dyeing box (2); the drive device is connected with the dyeing box (2) to drive the dyeing box to shake; the liquid inlet port is connected with the liquid outlet port by a liquid inlet conduit; and a liquid inlet valve is installed on the liquid inlet conduit. By utilizing the gel electrophoresis dyeing device, the plurality of gel plates can be dyed simultaneously, loading and unloading of the gel plates are convenient, crush of the gel plates is avoided, contact of an operator with a hazardous substance is reduced simultaneously, and time and labor are saved. The gel electrophoresis dyeing device is safe and convenient.
Owner:BEIJING FORESTRY UNIVERSITY

Anti-rabies virus monoclonal antibody and preparation method and application

InactiveCN101560255AExperimental costs are highEasy to operateImmunoglobulins against virusesTissue cultureImmunoblot AnalysisHybridoma cell
The invention discloses an anti-rabies virus monoclonal antibody and a preparation method and an application, belonging to the field of biomedicine and particularly relating to the preparation of a monoclonal antibody capable of identifying rabies virus and the application. The monoclonal antibody of the invention is screened by indirect Enzyme-linked immunosorbent assay (ELISA), and specificity and affinity thereof combined with antigen are identified by methods such as polyacrylamide gel electrophoresis analysis, speckle ELISA, immunoblot analysis and the like. The anti-rabies virus monoclonal antibody of the invention can be applied in multiple testing methods of antigen of rabies virus and can be also applied in the preparation of rabies virus detecting kit. The anti-rabies virus monoclonal antibody is secreted by anti-rabies virus monoclonal antibody hybridoma cell strain 2C5 with the preservation number being CGMCC No.3014.
Owner:NANJING MEDICAL UNIV +1

Distributed multi-segmented reconfigurable traveling wave grids for separation of proteins in gel electrophoresis

Various traveling wave grids and electrophoretic systems, and electrode assemblies using such grids, are disclosed. A configuration in which a voltage potential is used to load a biomolecule sample against a grid is disclosed. A unique strategy of using multiple, reconfigurable grids in such systems is also described. The strategy involves initially conducting a broad protein separation and then selectively tailoring one or more grids, and conducting one or more secondary processing operations. Related strategies and specific methods are additionally disclosed for separating samples of biomolecules and components thereof using the noted systems, assemblies, and grids.
Owner:PALO ALTO RES CENT INC

Deafness susceptibility gene screen test kit

The invention relates to a hereditable deafness susceptibility gene screen test kit. The kit uses mutation from C to T of 1,494 locus of a 12s rRNA gene, mutation from A to G of 1,555 locus of the 12srRNA gene, mutation from A to G of IVS7(-2) locus of an SLC26A4 gene and C(+ / -) of 235 locus of a GJB2 gene as detecting objects, designs and optimizes a set of specific primers respectively againsteach locus to be tested according to the PCR technical principle of a tetra-primer amplification refractory mutation system, amplifies the whole set of the primers in the same reaction tube, and performs primary multiple PCR amplification and primary gel electrophoresis on the four reaction tubes to obtain gene types of four loci simultaneously.
Owner:GENERAL HOSPITAL OF PLA

Compact cell clamp for slab gel plate assembly

Parallel plate slab gel enclosures (slab gel cassettes) used for vertical slab gel electrophoresis are secured to a frame to form a chamber for an upper buffer solution, and the securement is achieved by a pair of clamps that compress the cassettes against the frame. The clamps are preferably mounted to the frame in a pivotal connection that enables the user to easily rotate the clamps in and out of their clamping positions. An additional preferred feature is a pin protruding from each clamp in a position causing the pint to move upward against the bottom of the cassette and push the cassette upwards as the clamp is being engaged. This is particularly useful for cassettes formed of plates of unequal height where the shorter plate fits under an inverse shoulder on a gasket which thereby seals against both plates.
Owner:BIO RAD LAB INC

Crosslinking of swellable polymer with pei

InactiveUS20140144628A1Stable and labile crosslinkersResist erosionFluid removalFlushingFlocculationPapermaking
The invention is directed to stable and labile crosslinked water swellable polymeric microparticles that can be further gelled, methods for making same, and their various uses in the hygiene and medical arts, gel electrophoresis, packaging, agriculture, the cable industry, information technology, in the food industry, papermaking, use as flocculation aids, and the like. More particularly, the invention relates to a composition comprising expandable polymeric microparticles having labile crosslinkers and stable crosslinkers, said microparticle mixed with a fluid and an unreacted tertiary crosslinker comprising PEI or other polyamine based tertiary crosslinker that is capable of further crosslinking the microparticle on degradation of the labile crosslinker and swelling of the particle, so as to form a stable gel. A particularly important use is as an injection fluid in petroleum production, where the expandable polymeric microparticles are injected into a well and when the heat and / or pH of the well cause degradation of the labile crosslinker and when the microparticle expands, the tertiary crosslinker crosslinks the polymer to form a stable gel, thus diverting water to lower permeability regions and improving oil recovery.
Owner:UNIVERSITY OF KANSAS +1

Truncated fragments of alpha-synuclein in Lewy body disease

The application identifies fragments of alpha-synuclein in patients with Lewy Body Disease (LBD) and transgenic animal models thereof. These diseases are characterized by aggregations of alpha-synuclein. The fragments have a truncated C-terminus relative to full-length alpha-synuclein. Some fragments are characterized by a molecular weight of about 12 kDa as determined by SDS gel electrophoresis in tricine buffer and a truncation of at least ten contiguous amino acids from the C-terminus of natural alpha-synuclein. The site of cleavage preferably occurs after residue 117 and before residue 126 of natural alpha-synuclein. The identification of these novel fragments of alpha-synuclein has a number of application in for example, drug discovery, diagnostics, therapeutics, and transgenic animals.
Owner:TRUSTEES OF FLINDERS UNIV +2

Transilluminator base and scanner for imaging fluorescent gels, charging devices and portable electrophoresis systems

Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed. Also disclosed are visible light transillumination systems for viewing a pattern of fluorescence emitted by fluorophores comprising a cassette housing fluorophore-containing material and a base unit to support the cassette. In some aspects the base unit that includes a power supply also houses a light source having output in the visible wavelength region and a filter placed between the light source and the fluorophores. The system is constructed and arranged such that patterns of fluorescence emitted by the fluorophores are viewable. Also described are charging devices for providing charge to gel electrophoresis systems, portable gel electrophoresis systems and methods of use thereof.
Owner:LIFE TECH ISRAEL

Method used for detecting HLA-B*5801 alleles

The invention belongs to the field of pharmacogenomics and genetic diagnosis, and relates to a method used for detecting HLA-B*5801 alleles. The method comprises following steps: a DNA sample to be detected is taken, three pairs of specific primers and a pair of internal primers are taken, amplification of DNA segments is realized by using sequence specific primer method, and then the results of the amplification are analyzed by agarose gel electrophoresis; or sample DNA is extracted, a pair of specific primers, a pair of internal primers and three fluorescence probes are taken, amplification of DNA segments is realized by Taqman probe method using a fluorescence ration PCR instrument, and then the amplification curve is analyzed so as to obtain results. Results analysis methods such as agarose gel electrophoresis, high resolution melting curve and SYBRGreen fluorogenic quantitative PCR are employed in the method. The method has advantages of speediness, convenience, flexibility, high resolution and no contamination; is suitable for detection of HLA-B*5801 alleles in samples such as peripheral blood and hair; and can be used for determining the probability of severe skin adverse reaction of patients with gout or hyperuricemia caused by taking of allopurinol.
Owner:安徽同科生物科技有限公司
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