Gel electrophoresis dyeing device

Abstract

The invention discloses a gel electrophoresis dyeing device, which is characterized by comprising a liquid storage device (1), a dyeing box (2) and a drive device (4), wherein the liquid storage device is used for storing a liquid reagent used in the dyeing process and is provided with a liquid pouring port and a liquid outlet port; the top of the dyeing box is sealed by a dyeing box cover plate (20); a liquid outlet port is formed on the dyeing box cover plate (20); a gel plate fixing device (3) used for fixing a plurality of gel plates is arranged in the dyeing box (2); the drive device is connected with the dyeing box (2) to drive the dyeing box to shake; the liquid inlet port is connected with the liquid outlet port by a liquid inlet conduit; and a liquid inlet valve is installed on the liquid inlet conduit. By utilizing the gel electrophoresis dyeing device, the plurality of gel plates can be dyed simultaneously, loading and unloading of the gel plates are convenient, crush of the gel plates is avoided, contact of an operator with a hazardous substance is reduced simultaneously, and time and labor are saved. The gel electrophoresis dyeing device is safe and convenient.

Description

technical field [0001] The invention relates to the technical field of gel staining, in particular to a device for gel electrophoresis staining. Background technique [0002] Polyacrylamide gel electrophoresis, abbreviated as PAGE (Polyacrylamide gel electrophoresis) electrophoresis, is a commonly used electrophoresis technique using polyacrylamide gel as a supporting medium, with extremely high resolution. With the development of proteomics and molecular biology, polyacrylamide gel electrophoresis analysis technology has been applied more and more in the separation of proteins and small molecular weight PCR products, and has become indispensable in the analysis of protein and molecular markers. Technology, often used to separate proteins and screen single-stranded DNA base shifts, insertions or deletions and other genetic variations, can separate DNA molecules with a length difference of only 0.1%, suitable for RAPD, ISSR, SSR, AFLP, SRAP, cDNA -Detection of small fragment...

AI Technical Summary

Problems solved by technology

[0015] The traditional silver staining method can only be performed on a single gel plate for each staining and color development, which is inefficient and not conducive to the development of a large amount of analysis work; Putting the plate, pouring liquid, and adding liquid, in addition to causing pollution to the experimental console and the environment, frequent taking out and putting in operations may easily cause glass plates or gels to break; the operation is extensive and carried out in an open state. The dyeing box is an open rectangular plastic box. All kinds of dyeing solutions used in the dyeing process are directly poured into the plastic box. Because the formaldehyde and glacial acetic acid used in the dyeing process have a strong pungent smell, As a result, the entire operation site is filled with a strong and pungent smell, and the operators have to wear masks to operate; in addition, silver nitrate is easy to decompose when exposed to light, and the open dyeing environment is likely to cause the decomposition of silver nitrate during the dyeing process and make the dyeing incomplete; In addition, it will take a certain amount of time to take out, put in, and add liquid during the manual operation, prolonging the entire dyeing operation time; formaldehyde, polyacrylamide, etc. are toxic, and open liquid addition and non-stop The input operation will cause the operator to frequently contact with harmful and toxic substances; in addition, during the experiment, there will be differences in the amount of liquid added, the length of processing time, etc. due to different operators or the same operator in different operating steps, resulting in The difference in staining results between different batches of gels makes it difficult to guarantee the repeatability, which is not conducive to the comparative study between different batches of samples and reduces the accuracy of the experimental results; the traditional silver staining method has many steps, takes a long time, and requires There is a dedicated person to operate continuously, and the operation workload is heavy

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