136 results about "Electrophoresis technique" patented technology

The present invention relates to a method for separating biological macromolecular component by using two-dimensional or multidimensional capillary electrophoresis and its interface. The biological macromolecular component undergone the process of one-dimensional capillary electrophoresis separation can be passed through the interface and fed into two-dimensional capillary electrophoretic separation operation to implement two-dimensional capillary electrophoresis, the small molecular substance can be diffused and freely permeated through the hollow fibre in the interface and can obtain balance in it, and when the described biological macromolecular component is flowed through the interface, the buffer solution containing low molecular substance can be on-line added / removed into the hollowfibre in the interface.

The invention discloses a quantum dot composition and a preparation method thereof. Ligands of quantum dot particles are protected by modifying the outsides of the quantum dot particles with a metal organic framework material and / or a covalent organic framework material, the preparation process is simple, and the stability of the quantum dot particles is improved. In addition, the invention also discloses a quantum dot patterning method and a patterned quantum dot solid film prepared by the quantum dot patterning method, so as to solve the problem of relatively low preparation efficiency in amethod for patterning quantum dots by utilizing an electrophoresis technology at present.

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The invention discloses a method for constructing a wheat SSR fingerprint, which belongs to the field of agricultural wheat breeding and application technologies. The method comprises the following steps: extracting DNA (deoxyribonucleic acid) of a wheat variety to be tested; carrying out PCR (polymerase chain reaction) amplification to the DNA Of the wheat variety to be tested by using 13 pairs of basic core SSR fluorescent dye primers with the ID No.1 to 26 and shown in a sequence table SEQ and 12 pairs of extended core SSR fluorescent dye primers with the ID No.27 to 50 and shown in the sequence table SEQ respectively; and detecting PCR amplification products by denaturing polyacrylamide gel electrophoresis or five-color fluorescent capillary electrophoresis, and constructing the SSR fingerprint of the wheat variety. The method provided by the invention can identify the wheat variety in a laboratory by using technologies of the PCR amplification, the gel electrophoresis and capillary electrophoresis according to the polymorphism of SSR sites, can greatly improve the test efficiency in time, and has the advantages of high speed, high accuracy, convenience for operation and the like; and the identification result is not liable to be affected by the environment.

The invention discloses a magnet-based capillary electrophoresis electrochemical detection electrode locating device and belongs to the technical field of capillary electrophoresis. The device comprises an electrode bracket containing a magnet, a detection electrode, a capillary, a detection pool, a capillary locating pipe, an electrode locating pipe, a screw rod and a micrometer head, wherein the micrometer head is fixed on a bottom plate through an installing sleeve, the outer diameter of the screw rod is the same as the diameter of a circular hole in the electrode bracket, and the top end of the screw rod is attached to the circular hole through the magnet and can rotate freely; the capillary locating pipe and the electrode locating pipe are respectively installed on two side walls of the detection pool and are concentrically aligned, the capillary is fixed in the locating pipe, the detection electrode is installed in the small hole of the electrode bracket and is aligned with the capillary after being inserted into the locating pipe, the electrode bracket drives the detection electrode to move back and forth by rotating a knob of the micrometer head so as to realize the precise adjustment on the space between an outlet of the capillary and the detection electrode.

Colored fluids for electrowetting, electro fluidic, or electrophoretic devices, and the devices themselves, are disclosed. The colored fluid can include a non not aqueous polar solvent having (a) a dynamic viscosity of 0.1 cP to 50 cP at 250C, (b) a surface tension of 25 dynes / cm to 55 dynes / cm at 250C, and (c) an electrowetting relative response of 40% to 80%. Such colored fluids further include a colorant selected from a pigment and / or a dye. In another embodiment, the colored fluid can include a non-polar solvent and an organic colorant selected from a pigment and / or a dye. Such colored fluids can be black in color and have a conductivity from 0 pS / cm to 5 pS / cm and a dielectric constant less than 3. The use of the colored fluids offers improvements in reliability, higher levels of chroma in the dispersed state, and the ability to achieve higher contrast ratios in display technologies.

The invention provides a rice fingerprint constructing method. The method includes: extracting rice genome DNA, screening SSR fluorescent primers with high polymorphism, subjecting the rice variety DNA to PCR amplification, detecting the PCR product by utilization of capillary gel electrophoresis, analyzing data to obtain a 0-1 matrix graph and converting the 0-1 matrix graph into a fingerprint mode mapping. By adoption of the characteristics of the SSR polymorphism and the high resolution of the capillary gel electrophoresis technology, the method can recognize two polymorphism bands with a band difference being 1 bp. The method is prone to automation, simple in operation, stable and reliable in results, and prone to large-scale multi-batch data integration and analysis, and is suitable for construction of a large number of rice variety fingerprints.

The invention relates to chiral separation chromatographic and electrophoretic techniques. The aim of said invention is to obtain chiral stationary and mobile phases comprising an oligonucleotide which is specifically selected by a SELEX method against an enantiomer to be separated as a special-purpose chiral selector. Methods for separating enantiomers by the chiral stationary and mobile phases are also disclosed.

The invention provides a primer, a kit and a method for rapid identification of pigeon gender. The primer shown in the SEQ ID NO:1 and SEQ ID NO:2 has the advantages of high specificity, high accuracy, high resolution and the like in design of CHD gene sequence in pigeons; the amplification result is directly carried out by agarose gel electrohoresis, which provides higher identification so that the amplification result is intuitive and clear, and the pigeon gender can be easily distinguished. The invention further provides a molecular identification method of the pigeon gender by the aid of the primer. In the method, efficient nucleic acid lysate is further provided on the basis of the above the specific amplification of the primer, the process of obtaining DNA from feather pulp samples is greatly simplified, and time and economic cost in identification of the pigeon gender are reduced. The method and the kit for identification of pigeon gender with the PCR (polymerase chain reaction)-agarose electrophoresis technology and a genotyping method are simple in operation, rapid in identification, low in cost and abroad in the market utilization prospect.

The invention provides a kit using single cell gel electrophoresis for detecting cell DNA damage and a method. The method includes gel laying, splitting, unwinding, electrophoresis, neutralizing and washing, drying and dyeing, a fluorescence microscope is used for observing a dyeing result and taking photos, and the damage degree of cell DNA is analyzed and evaluated. As three layers of gel need to be laid on a glass slide during sample preparation, a traditional single cell gel electrophoresis technology has the disadvantages of being complex in operation and prone to degumming, and sample images are unclear; while only one layer of gel needs to be laid on a glass slide, the kit has the advantages of being simple in sample preparing process, low in degumming rate, easy to operate, low in experiment cost and the like.

The invention provides a genetic analysis method used for rice. According to the method, capillary gel electrophoresis is utilized to detect SSR-PCR amplification products, and the high resolution characteristic of the capillary gel electrophoresis technology is utilized to distinguish rice varieties with near genetic relationship, so that analysis results are more accurate. By adoption of the genetic analysis method based on capillary gel electrophoresis and SSR labeling, a dendrogram established by UPGMA clustering analysis is used for genetic relationship analysis of rice varieties. The genetic analysis method has advantages of high resolution, rapidness, high efficiency, accurate and reliable results, and the like. The method has characteristics of easy automation and convenience for integration and analysis of a large amount of data, which are beyond the grasp of traditional detection methods.

The invention relates to an Off-Adhesive free flow electrophoresis coupling chip and a making method thereof, belonging to the technical field of microfluidic chip manufacture. The chip comprises a chip substrate, an electrode and a separating chamber, wherein an immobilized pH gradient adhesive tape area and hard substrates for fixing and embedding of immobilized pH gradient adhesive are arranged at the tail end of the separating chamber. The method comprises the following steps: arranging a rectangular area for embedding of the immobilized pH gradient adhesive tape at the tail end of the separating chamber of the other substrate, then aligning the chip substrate with another substrate, carrying out sealing connection on the two substrates and the electrode according to a combined sealing method of the free flow electrophoresis chip to form a free flow electrophoresis chip, arranging the chosen immobilized pH gradient adhesive tape at the rectangular slotting area of the chip, and pressing and fixing the immobilized pH gradient adhesive tape by using the other hard substrate for using. The chip not only solves the defects of the free flow electrophoresis chip and Off-Adhesive electrophoresis technologies, but also inherits the advantages of the two technologies; and the chip has simple manufacturing method, and the immobilized pH gradient adhesive tape is replaceable.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

The invention discloses a micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and a method for preparing the system, and relates to the micro-fluidic chip electrophoresis technique. The system takes water as solvent, takes MES-Tris as background electrolyte, takes hydroxypropyl cellulose, hydroxypropylmethylcellulose, glycol cellulose, polyvinyl alcohol or polyoxyethylene and two to five compounds as sieving media, and takes glucose, glycerol, mannitol, or chitosan and two to four compounds as additives. The method for preparation comprises the following steps: firstly, preparing the background electrolyte, secondly, adding the sieving media, and thirdly, adding additives. The micro-fluidic chip electrophoresis non-glue sieving system of gene diagnosis and the method for preparing the system have convenient and safe use, high separation efficiency and excellent effect, and is suitable for separating PCR products of clinical diseases, thereby making reference for diagnosing diseases.

This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

The invention relates to an extraction method of protein from animal tissues, in particular to an extraction method of total protein from a rumen epithelial tissue of a milk cow. The method comprises the steps of putting a rumen epithelial tissue sample of the milk cow in a precooled mortar, adding liquid nitrogen, grinding into powder, adding an acetone solution of precooled trichloroacetic acid, oscillating, eddying, uniformly mixing for overnight aging, centrifugally collecting sediment, freeze-drying the tissue sediment, adding lysate, incubating and eddying at intervals, and centrifugally collecting supernate, namely the total protein of the rumen epithelial tissue of the milk cow. For the total protein of the rumen epithelial tissue of the milk cow, extracted by the method, a total protein mixture can be separated by a two-dimensional gel electrophoresis technology according to an isoelectric point and molecular weight of the protein, the relative abundance of the protein is acquired, the isoelectric point, the molecular weight and a relative transcript level of each protein can be clearly and visually shown in a gel atlas, and the method has the advantages of more obvious integrity and visualization in comparison with the prior arts such as a high efficiency liquid chromatography separation method, an enzyme-linked immunosorbent assay and immunoblotting.

The invention provides a detection kit for an unstable state of a microsatellite. The kit comprises a primer group for detecting the unstable state of the microsatellite. The primer group is characterized in that upstream and downstream primers are designed for 10 microsatellite sites, namely NR21, D5S346, NR24, BAT25, D17S250, MONO27, BAT26, D2S123, Penta D and TPOX; base sequences of the primersare shown as SEQ ID NO.1 to 20; and the primers are high in specificity and wide in coverage range. The invention further provides a detection method of the unstable state of the microsatellite. Thedetection method is based on a multiplex fluorescence PCR technology and a capillary electrophoresis technology. The kit provided by the invention is used for detecting the unstable state of multiplemicrosatellite sites, and has the advantages of convenient and rapid operation, high specificity and sensitivity, good reproducibility, and accurate and reliable results.

The present invention relates to a detection method of gene promoter methylation. Said method includes the following steps: firstly, using freshly-prepared modification reagent to modify extracted DNA, after the modified DNA is passed through column, making purification and desulfurization reaction, then using absolute ethyl alcohol and salmon sperm DNA to enrich micro modified DNA, further selecting and using specific PCR GC Buffer to make multiple methylation specific PCR amplification, then adopting electrophoresis technique to analyze result.

The invention discloses a method for identifying whether wheat to be tested is Pina-D1b deficient wheat and applications thereof. The method according to the invention comprises the following steps: 1) using a specific primer pair for PCR amplification by taking genome DNA of the wheat to be tested as a template, wherein the specific primer pair is the primer pair composed of nucleotide sequences, which are shown in sequence 1 and sequence 2 of sequence table; and 2) detecting PCR-amplified product, wherein the wheat to be tested with 776bp strips in the amplified product is candidate Pina-D1b deficient wheat, and the Pina-D1b deficient wheat is the wheat having nucleotides, which are shown in sequence 3 of the deficient sequence table of the genome DNA. The specific primer pair according to the invention can take the place of traditional SDS-PAGE and two-dimensional electrophoresis technology and the detection for Pina-D1b type is directly carried out at molecular level, so accuracy and reliability are greatly enhanced, screening efficiency and accuracy for grain hardness genotype is remarkably quickened; in addition, the method plays an important role in improving gene engineering of wheat grain hardness, simultaneously aids in further research on genetics and molecular biology of wheat grain hardness and is also significant to the cultivation of high-quality wheat.

The invention discloses a method for manufacturing a three-dimensional member via local etching and a positioning tool. The method comprises: firstly, manufacturing the position tool according to the shape of a to-be-etched three-dimensional member; secondly, plating the surface, needing to be etched, of the to-be-etched three-dimensional member with a layer of photosensitive ink via electrophoresis technique; removing the photosensitive ink on a part, needing to be etched, of the surface of the to-be-etched three-dimensional member via laser etching technique to expose the part needing to be etched; thirdly, positioning the to-be-etched three-dimensional member on the positioning tool, putting the positioning tool in an etching machine, and performing corrosion of a set depth with a pre-set etchant solution; and finally, performing film removing, cleaning, and drying of the etched three-dimensional member to obtain a finished three-dimensional member. According to the invention, the positioning tool for manufacturing the three-dimensional member via local etching can enable positioning of the to-be-etched three-dimensional member to be realized via forming a containing groove according to the shape (size) of the to-be-etched three-dimensional member. The cost is saved; the precision of the three-dimensional member is relatively high; and the specification and dimension are comparatively accurate.

This invention relates to a determination method for wheat high molecular weight glutelin subunit, especially a method through technique of acid capillary electrophoresis. The key emphasis in current wheat breeding work is to improve the HMW-GS component, especially to improve the frequency of excellent high molecular weight glutelin subunit or subunit pairs The combination of current usual breeding method with label assistance selection techniques is the main means for wheat breeding improvement, wherein the rapid, microscale and accurate determination of excellent protein subunit for generation in the early hybrid time is the key to improve the breeding efficiency. The main task of this invention is to provide the buffer type and additives and condition parameter of acid capillary electrophoresis and clean-up procedures of capillary.

The invention discloses a method for two-dimensional electrophoresis extraction and separation of total protein of a rat and mouse testicular tissue sample. The method comprises rat and mouse testicular tissue treatment, a preparation method for a testicular tissue total protein sample, a two-dimensional electrophoresis technology system, gel dyeing and spectrum analysis. The method optimizes a tedious universal two-dimensional electrophoresis technology system with relatively poor stability, greatly reduces time and reagent consumption for finding out conditions, saves experiment cost, and obtains a satisfactory two-dimensional electrophoresis spectrum that is convenient for subsequent analysis. The method is more suitable for the extraction of the rat and mouse testicular tissue protein sample than a universal homogenate extraction method, a lysate extraction method, a lysate extraction / acetone precipitation method at present. The method not only reduces the degradation of the protein but also increases solubility of the protein without increasing cost of the reagent, so that the two-dimensional electrophoresis spectrum with relatively many protein sites, clear horizontal stripes and relatively good repeatability can be obtained.

The invention relates to an electrophoretic separation device and a technology used for laboratorial and large-scale separated and purified biological samples. The invention mainly relates to the electrophoretic separation device which is a device capable of being used for performing the non-denaturing electrophoretic preparation technology, and a method required for completing the electrophoretic preparation technology by the device. The device mainly comprises a cooling chamber which is provided with a good heat exchange structure, a vertical electrophoretic plate with non-denaturing polyacrylamide gel arranged in the cooling chamber, a concentrating and collecting chamber which is used for sample collection, and an electrophoretic cell. The technology mainly comprises screening of collection time of purified proteins and a method for collecting the purified proteins. The device and the technology have the characteristics of large processing capacity, high resolution, convenient operation and so on, can be used for preparing laboratorial and large-scale biological products and biological medicines, and have considerable economic benefit and social benefit.

The invention relates to a bilateral molecular marker identification technology of a melon wilt resistant variety. By the utilization of polymerase chain reaction and bilateral molecular markers ML430 and MR296 which are closely linked to a melon wilt resistance gene, specific DNA fragments are amplified. The size differences of amplified DNA fragments between a resistant variety and a susceptible variety can be displayed on different locations of a sepharose gel by electrophoretic technique. And the sizes and locations are compared with that of DNA fragments amplified from a standard resistant variety and a standard susceptible variety. Two varieties which have the same size or lie in the same location belong to the variety of the same type. When banding patterns of the two molecular markers both are resistance type, the variety is the resistant variety. The method provided by the invention has advantages of convenient sampling, precise identification, and no influence by plant growth and development and weather conditions. The technology provided by the invention is mainly used for the screening and identification of wilt resistance variety resources, and can also be used for molecular marker assisted breeding of melon.

The invention provides a primer combination for constructing a wolfberry DNA fingerprint spectrum, and applications and a method, belonging to the technical field of a DNA fingerprint spectrum. The method comprises the following steps: on the basis of wolfberry gene sequencing results, firstly developing and screening out 15 SSR primer pairs with polymorphism, wherein the 15 SSR primers can be used for constructing the wolfberry DNA fingerprint spectrum; and constructing the wolfberry DNA fingerprint spectrum by combining the capillary electrophoresis technology. The method has the characteristics of being high in electrophoresis resolution and sensitivity, portable in data analysis and processing and the like, thus providing a solid theory support for further application research of the DNA fingerprint spectrum on wolfberries.

The invention discloses a fluorescent detection kit for detecting 12 deafness predisposing genes simultaneously. The kit can detect 12 mutational hotspots in the most common deafness associated genes of the Chinese in 3 hours. The kit comprises reagents before amplification and reagents after amplification, wherein the reagents before amplification comprise a polymerase chain reaction (PCR) buffer solution, a reaction mixture of MgCl2 and deoxyribonucleoside triphosphates (DNTPs), Taq DNA polymerase, ultrapure water, and a primer mixture for amplifying loci of detection sites at high specificity; and the reagents after amplification comprise a genotyping standard and an internal standard. Deafness gene loci are simultaneously detected at high sensitivity and high specificity by combining a fluorescent labeling technology, a linolenic acid (LNA) nucleoside monomer doping-primer modification technology and a capillary electrophoresis technology for the first time, manpower and material resources and time are greatly saved, and pollution due to multi-step operation is prevented.

The invention provides preparation of dopamine-coated sea urchin-shaped manganese dioxide hollow microspheres, which comprises the following steps: completely dissolving manganese dioxide hollow microspheres in a tris (hydroxymethyl) aminomethane hydrochloride solution, then adding dopamine hydrochloride, carrying out ultrasonic treatment for 10-25 minutes, centrifuging, washing and drying to obtain the dopamine-coated manganese dioxide hollow microspheres. The hollow microspheres have the advantages of thermal stability, easiness in synthesis, porous structure, low density, short diffusion path, high specific surface area, rich active sites and the like, and moreover, the formed aminated manganese dioxide hollow microspheres are beneficial to immobilization of acetylcholin esterase, so that the aminated manganese dioxide hollow microspheres serving as a novel enzyme immobilization carrier have a good application prospect. After the acetylcholin esterase is immobilized, the capillary electrophoresis technology is combined, and the acetylcholin esterase inhibitor is screened from traditional Chinese medicines, so that the method has the characteristics of high separation efficiency, short analysis time, low sample consumption, low operation cost and the like, and a technical support is provided for discovery of new medicines for treating Alzheimer's disease.

The invention discloses a construction method of a corylus plant variety SSR molecular marker fingerprint spectrum. The construction method comprises the following steps of: (1) extracting the corylusplant DNA of a variety which participates in selection; (2) using 25 pairs of core SSR molecular marker primers to independently carry out multiple PCR amplification on a variety to be tested; (3) grouping an amplification product in (2), and then, carrying out mixing according to groups to carry out capillary electrophoresis detection; and (4) carrying out data analysis on a detection result, and constructing the fingerprint spectrum. In the construction method, a core primer is high in polymorphism, data can be easily read, each species of a corylus plant is compatible, and a distinguishingfunction is powerful. Through a primer mixing PCR technology, one-time PCR can obtain the products of two groups of primers, and one-multiple PCR cost is saved. Through a PCR product mixing capillaryelectrophoresis technology, the data information of four to eight groups of primers can be simultaneously obtained through one-time electrophoresis, three-to-seven-multiple capillary electrophoresisexpense is saved, and therefore, the purposes of saving cost for an experiment operation and being economic and quick is realized.

The invention provides a preparation method of a bone repair and tumor inhibition material with photothermal conversion performance and a functional coating and the material obtained by the method. The preparation method comprises the following process steps: (1) taking polyether-ether-ketone powder and conductive and photothermal conversion material powder as raw materials, uniformly mixing the two kinds of powder, and processing the formed mixed powder into a required shape, so as to obtain a matrix with conductivity and photothermal conversion performance; (2) grafting an active functionalgroup on the surface of the matrix with conductivity and photothermal conversion performance, then putting the matrix with the surface grafted with the active functional group as an electrode into a dispersion liquid, and forming a functional coating on the surface of the matrix through an electrophoresis technology, the dispersion liquid being composed of a liquid conductive medium, an osseointegration promoting substance and a dispersing agent; and (3) cleaning the material with the functional coating after electrophoresis with deionized water, and drying the material to remove water on thesurface, thereby obtaining the bone repair and tumor inhibition material with the photothermal conversion performance and the functional coating.