31 results about "Glycomics" patented technology

Systems and methods of quantifying a glycomic parameter, a genomic parameter, a proteomic parameter, a metabolic parameter, and / or a lipidomic parameter of a biological sample; obtaining a clinical parameter associated with a subject from which the one or more biological samples originated; determining one or more relationships between one or more of: (i) one or more of the quantified glycomic parameters, genomic parameters, proteomic parameters, metabolic parameters, and lipidomic parameters, (ii) a predetermined range associated with one or more of the quantified glycomic parameters, genomic parameters, proteomic parameters, metabolic parameters, and lipidomic parameters, and (iii) an obtained clinical parameter; identifying one or more biomarkers based on one or more of the determined relationships satisfying a predetermined significance criteria; and / or determining a wellness classification state of a wellness classification, the determination of the wellness classification state determined based on the one or more identified biomarkers.

Methods and reagents for labeling molecules of interest in a plurality of samples, and then combining and selecting labeled molecules away from unlabeled molecules for use in simultaneous co-assaying analysis. The reagents comprise labeling means of distinguishable radioactive isotopes which remain with the labeled molecules. Additionally, the reagents also comprise selection means which can be affinity tags, beads, or immobilized surface which may remain or be cleaved off through cleavable linkers. A set of labeling reagent can be used to label a plurality of samples, combine them before or after selecting / enriching for labeled molecules and co-assay together for reliable comparison. This invention has many applications in comparing and panning for differentially abundant molecules or differential modification of molecules for proteomics, glycomics, phospho-proteomics, metabolomics, epi-genomics . . . studies.

The invention relates to a preparation method for a monoclonal antibody library of tumor mucoprotein glycopeptide epitope. The method comprises the following steps of: extrapolating all possibly occurring tumor glycopeptide sequences according to an amino acid sequence of mucoprotein; then transfecting tumor cells by using mucoprotein genes to obtain immunogens for immuning a mice to prepare monoclonal antibodies; synthesizing all extrapolated glycopeptides by using a chemical synthesis method and labeling with biotins respectively to obtain the biotin-labeled glycopeptides for screening the monoclonal antibodies; and finally, immuning the mice by using the immunogens to prepare hybridoma, screening the hybridoma by using the biotin-labeled glycopeptides, and sorting out the monoclonal antibody aiming at each specific glycopeptide structure. In the invention, an immune glycomic method is used for the first time to extrapolate all possibly occurring epitopes of highly glycosylated tandem repeat polypeptide segments during cancerization. The glycopeptide antigens are obtained by using the chemical synthesis method; and then the synthesized glycopeptides are used for tagging the monoclonal antibodies.

The invention belongs to the technical field of analytical chemistry and particularly relates to an N-sugar chain relative quantitation method based on a 18O mark, concretely comprising the following steps of: mixing an N-sugar chain marked by an isotope 18O produced by catalysis of endoglycosidase with an unmarked N-sugar chain produced by catalysis of the endoglycosidase into a solution according to an equal molar ratio, detecting the peak intensity by biological mass spectrometry, and carrying out relative quantitation after eliminating isotope peak overlapping through calculation. The invention successfully solves the problem of isotope peak overlapping of the N-sugar chain marked by the isotope 18O and the unmarked N-sugar chain in the mass spectrometry, obtains good linearity and lower variation coefficient within a two magnitude order dynamic range, and supplies an efficient revise and calculation method to application of the method for marking the N-sugar chain by the isotope through catalysis of the endoglycosidase in quantitative glycomics.

The invention belongs to the field of sugar proteomics and glycomics analysis, and relates to a method for using phosphoro-amidate for deriving an N-carbohydrate chain and using Ti4+modifiaiton magnetic nanoparticles for enriching the derived N-carbohydrate chain. The method comprises the steps that firstly, phosphate radicals are introduced to the N-carbohydrate chain derived from the phosphoro-amidate at the reducing end of the N-carbohydrate chain, then the Ti4+modifiaiton magnetic nanoparticles Fe3O4@Ti4+ are placed into a derived carbohydrate chain solution, the carbohydrate chain is fixed to the magnetic nanoparticles through a chelation reaction between the phosphate radicals in the Ti4+ and the carbohydrate chain, non-carbohydrate chain molecules (such as protein, polypeptide, inorganic salt and the like) not combined with the nanoparticles are cleaned away and removed, and finally the captured carbohydrate chain is disengaged from the materials on the alkaline condition and fed into a mass spectrometry carbohydrate chain. The method is simple in steps, convenient to implement, rapid, efficient and capable of achieving high-sensitive and high-selective mass spectrometry of the N-carbohydrate chain.