30 results about "Glycomics" patented technology

The invention belongs to the technical field of biology and relates to a method for multiply detecting a carbohydrate chain structure of glycoprotein through an antibody-assisted lectin liquid-phase suspension chip. According to the method, lectins are coupled to magnetic beads marked with different dyes, the magnetic beads which are coupled with different lectins are identified through a Bioplex detection system, and different glycoforms on the same protein can be simultaneously detected by adding the magnetic beads which are coupled with different lectins and have different serial numbers into a reaction, so that the detection flux of the carbohydrate chain structure is remarkably improved. According to the method, the carbohydrate chain structure on the glycoprotein can be quickly and specifically detected with high flux. The method can be further applied to detection of a clinical biological marker and provides an efficient way for research on disease glycomics.

Business methods useful for identifying, and marketing or commercializing glycomics-related diagnostic, therapeutic and/or imaging probe products are disclosed. Patient test samples are screened for the presence of glycan-binding moieties to produce binding data. Binding data is collected into a database. One or more bioinformatic algorithms are used to process the collected binding data to identify one or more diagnostic, therapeutic or imaging probe products. Identified products are collaboratively or independently, marketed or commercialized. Business methods are also provided for marketing or commercializing products for producing oligosaccharides, reactive antibody products, and monoclonal antibody cocktail products. Business methods of conducting glycomics-related cancer trials are also disclosed.

The invention belongs to the field of the glycoproteomics and glycomics, and relates to a method for rapid enzymolysis release, solid phase concentration and mass spectrometry of N-glycan. The methodcomprises the following steps of absorbing a solution containing a glycoprotein sample by using a degreasing cotton material, and performing high-temperature rapid PNGase F enzymolysis on the cotton to release N-glycan; adjusting solution environment after the enzymolysis is finished, and adsorbing free glycan on the degreasing cotton material by utilizing the high-density hydroxy and hydrogen bond of the glycan and like effects on the surface of the degreasing cotton material, and then cleaning and removing non-glycan molecules such as protein, polypeptide and like which are not bonded with the degreasing cotton material, and finally eluting the adsorbed glycan with the aqueous solution, and feeding into mass spectrometry. Through the method disclosed by the invention, the used material is cheap and easy to obtain, the preparation is easy, the operation is simple and convenient, the high-speed enzymolysis of the N-glycoprotein and the high-selective concentration and high-sensitive mass spectrometry of the N-glycan can be realized.

The invention belongs to the field of medicines, and relates to an application of a method for degrading all carbohydrate chains in the blood to be monosaccharide and detecting the monosaccharide in cancer detection. A sample in the method for degrading the blood to obtain the monosaccharide and detecting the monosaccharide is the blood, and the cancer includes lung cancer, gastric cancer, ovarian cancer, penis cancer, esophagus cancer, oral cancer, biliary duct cancer, breast cancer, adenocarcinoma perampullaire, rectal cancer and bladder cancer. The method has the characteristics of simplicity and easiness in operation steps, easiness in popularization, short detection time, low requirement on the instrument, low detection cost, less consumption of blood and the like. The result displays that based on the content of eight monosaccharides in the blood, not only can a normal person and a cancer patient be distinguished, but also different cancers can be distinguished. The method for simplifying glycomics knowledge and applying in the detection of the biomarker of blood is first created in the world.

The invention discloses isotope-labeled bionic sugar or sugar group and a preparation method and applications thereof. Specifically, the isotope-labeled bionic sugar or sugar group disclosed by the invention include a reducing alcohol hydroxyl located at the reducing end of the sugar chain and an isotope label, wherein the molecular weight of each bionic sugar chain is increased by 3 Daltons compared with that of corresponding unmodified sugar chain, and the bionic sugar (group) has the same sugar chain composition and similar sugar chain abundance as the unmodified sugar (group). The invention also provides a method for analyzing the sugar group in a sample by using the isotope-labeled bionic sugar or the sugar group, and the method comprises the step: carrying out mass analysis on the mixture of the isotope-labeled internal standard bionic sugar chain and the sample sugar chain which is not labeled by the isotope so as to carry out qualitative and/or quantitative analysis on the sugar group in the sample. The method has the advantages of both the simple operation of a non-isotope-labeled glycomics method and the accuracy of an isotope-labeled glycomics method, can be used for high-throughput detection, and has wide application prospect.

The invention relates to a preparation method for a monoclonal antibody library of tumor mucoprotein glycopeptide epitope. The method comprises the following steps of: extrapolating all possibly occurring tumor glycopeptide sequences according to an amino acid sequence of mucoprotein; then transfecting tumor cells by using mucoprotein genes to obtain immunogens for immuning a mice to prepare monoclonal antibodies; synthesizing all extrapolated glycopeptides by using a chemical synthesis method and labeling with biotins respectively to obtain the biotin-labeled glycopeptides for screening the monoclonal antibodies; and finally, immuning the mice by using the immunogens to prepare hybridoma, screening the hybridoma by using the biotin-labeled glycopeptides, and sorting out the monoclonal antibody aiming at each specific glycopeptide structure. In the invention, an immune glycomic method is used for the first time to extrapolate all possibly occurring epitopes of highly glycosylated tandem repeat polypeptide segments during cancerization. The glycopeptide antigens are obtained by using the chemical synthesis method; and then the synthesized glycopeptides are used for tagging the monoclonal antibodies.

Systems and methods of quantifying a glycomic parameter, a genomic parameter, a proteomic parameter, a metabolic parameter, and/or a lipidomic parameter of a biological sample; obtaining a clinical parameter associated with a subject from which the one or more biological samples originated; determining one or more relationships between one or more of: (i) one or more of the quantified glycomic parameters, genomic parameters, proteomic parameters, metabolic parameters, and lipidomic parameters, (ii) a predetermined range associated with one or more of the quantified glycomic parameters, genomic parameters, proteomic parameters, metabolic parameters, and lipidomic parameters, and (iii) an obtained clinical parameter; identifying one or more biomarkers based on one or more of the determined relationships satisfying a predetermined significance criteria; and/or determining a wellness classification state of a wellness classification, the determination of the wellness classification state determined based on the one or more identified biomarkers.

The invention relates to the technical field of bioglycomics analysis, and discloses a general low-cost quaternary ammonium salt sugar chain isotope labeling reagent and a synthesis method thereof. The reagent comprises a labeling reagent deuterated 7-4-methyl-1-(2-hydrazino-2-oxoethyl)-pyridinium bromide in a heavy isotope form and a labeling reagent 4-methyl-1-(2-hydrazino-2-oxoethyl)-pyridinium bromide corresponding to the labeling reagent deuterated 7-4-methyl-1-(2-hydrazino-2-oxoethyl)-pyridinium bromide in a light isotope form. The reagent can be used for mass spectrum high-sensitivity and high-accuracy relative quantitative analysis of biological reducing sugar chains of different molecular weight segments; according to the reagent provided by the invention, the mass spectrometric detection sensitivity of the sugar chain can be greatly improved; meanwhile, the method is suitable for mass spectrum relative quantitative analysis of reducing sugar chains with relatively small and large molecular weights; the synthesis raw materials are low in cost, and the defect that hydrazide quaternary ammonium salt stable isotope labeling reagents synthesized based on deuterated isoquinoline are high in cost is overcome.

The invention belongs to the technical field of glycomics, and discloses an N-sugar chain structure identification Denovo method and system based on mass spectrometric data, and the method comprises the following steps: extracting structure and composition information of sugar chain fragment ions in the mass spectrometric data, and carrying out N-sugar chain identification based on a basic peak, a cross peak and a generalized monosaccharide dictionary, and reducing the search space of the identification result candidate structure by using a pruning strategy to obtain an N-sugar chain structure corresponding to the mass spectrum. On the basis of mass spectrum data of the N-sugar chain and the idea of Denovo, the structure and composition information of sugar chain fragment ions in the mass spectrum data is extracted, and the N-sugar chain structure corresponding to the mass spectrum is identified. In the identification process, basic peaks and cross peaks are introduced, and N-carbohydrate chain identification is carried out based on the basic peaks and the cross peaks; a generalized monosaccharide dictionary is introduced, the spectrogram quality is improved, and the robustness of the identification method to noise in mass spectrum data is improved; and reducing the search space of the identification result candidate structure by using a pruning strategy. According to the invention, the mass spectrum identification quality is improved.

Creating synthetic biological data for a subject may include: (a) receiving a real biological data tag derived from a biological sample of the subject; (b) creating an input vector based on the real biological data tag; (c) inputting the input vector into a machine learning platform; (d) generating a predicted biodata tag for the subject based on the input vector, wherein the predicted biodata tag comprises synthetic biodata specific to the subject; and (e) preparing a report comprising the synthetic biological data of the subject. The biological pathway activation tag may be genomic, transcriptomic, proteomic, metabonomic, lipidomic, glycomic, methylomic, or secretomic. A potential code of the input vector in a potential space of the machine learning platform is adjusted with at least one constraint of an attribute of the subject such that the predicted biodata tag is based on the at least one constraint.

The invention discloses a sugar chain relative quantitative isotope labeling mass spectrum derivatization reagent, and particularly relates to a d0-DPBOC reagent and a d5-DPBOC reagent. The structureof the reagent contains an N-hydroxysuccinimide activated carboxyl structure so that a sugar chain of glycoprotein digested by streptavidin E can be labeled under an alkaline condition, and the retention of the labeled sugar chain in a reversed-phase chromatographic column is enhanced. Besides, the reagent contains a d0/d5 stable light and heavy isotope benzoyl (d0/d5-Benzoyl acyl) structure, relative quantitative analysis of the sugar chain can be carried out based on an isotope ratio (d0/d5) by utilizing a liquid chromatography-mass spectrometry technology, and a sugar chain standard substance is not needed. A novel high-sensitivity isotope mass spectrum derivatization reagent is provided for research of differential glycomics and screening of new biomarkers of disease carbohydrate chains.

A sample preparation workflow for promoting deep N-glycomics analysis of human serum by capillary electrophoresis (CE-LIF) with laser-induced fluorescence detection adapts to higher sample concentration requirements of electrospray ionization mass spectrometry (CE-ESI-MS) linked to capillary electrophoresis. A temperature gradient denaturation scheme is applied to amine functionalized magnetic bead dispensed glycoproteins to avoid precipitation. This also results in a significant reduction in free sugar content in serum, which results in the release of PNGase F mediated N-linked carbohydrates. The released oligosaccharide is labeled with aminopyrene-trisulfonate using an improved evaporation labeling scheme. The workflow provides an appropriate amount of material, for example, for CE-ESI-MS analysis performed in an anionizing mode.