Isotope-labeled bionic sugar or sugar group and preparation method and applications thereof

Abstract

The invention discloses isotope-labeled bionic sugar or sugar group and a preparation method and applications thereof. Specifically, the isotope-labeled bionic sugar or sugar group disclosed by the invention include a reducing alcohol hydroxyl located at the reducing end of the sugar chain and an isotope label, wherein the molecular weight of each bionic sugar chain is increased by 3 Daltons compared with that of corresponding unmodified sugar chain, and the bionic sugar (group) has the same sugar chain composition and similar sugar chain abundance as the unmodified sugar (group). The invention also provides a method for analyzing the sugar group in a sample by using the isotope-labeled bionic sugar or the sugar group, and the method comprises the step: carrying out mass analysis on the mixture of the isotope-labeled internal standard bionic sugar chain and the sample sugar chain which is not labeled by the isotope so as to carry out qualitative and / or quantitative analysis on the sugar group in the sample. The method has the advantages of both the simple operation of a non-isotope-labeled glycomics method and the accuracy of an isotope-labeled glycomics method, can be used for high-throughput detection, and has wide application prospect.

Description

technical field [0001] This application belongs to the fields of biotechnology, analytical chemistry technology and medicine. Specifically, this article relates to isotope-labeled biomimetic sugars or glycans, their preparation methods and applications, especially a simple, high-throughput, and accurate stable isotope internal standard glycan analysis method. Background technique [0002] Glycosylation is a ubiquitous post-translational modification that not only affects protein structure, solubility, and stability, but also involves multiple biological processes, such as protein folding, cell recognition, and receptor-ligand binding. Studies have reported that about 50% of mammalian proteins undergo glycosylation modifications. Abnormal glycosylation of glycoproteins is closely related to many diseases, including arthritis, congenital diseases, and tumor development and metastasis. [0003] In recent years, considering the advantages of body fluid samples, people have beg...

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Problems solved by technology

[0009]However, the current quantitative methods of isotope labeling have their own disadvantages: enzymatic hydrolysis to introduce isotope labeling can only introduce a molecular weight difference of 2 Da, which requires additional deconvolution calculations; The introduction of isotope labels is only applicable to cell samples, and the experimental cost is high; while most of the current chemical derivatization methods for introducing isotope labels are cumbersome, all samples need to undergo the same derivatization treatment, and samples of the same group usually need to be mixed together , and then compared with another group of mixed samples, it is impossible to quantitatively analyze each sample individually

However, due to the heterogeneity, broad molecular weight range, and abundance of abundance in natural glycan structures, it is ideal to provide an internal standard that possesses a glycan composition and abundance similar to the glycans to be analyzed features, but currently this remains a challenge

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