7380results about "Sugar derivatives preparation" patented technology

The invention relates to the technical field of oil exploitation, and discloses a compound containing an amide group as well as a preparation method and application thereof. The compound containing the amide group has a structure as shown in a formula (1). The polymer obtained by polymerizing the compound can improve the water-drive recovery efficiency of conventional oil reservoirs and common heavy oil reservoirs. # imgabs0 #

The invention relates to the field of preparation of novel compounds, and discloses a glycoside group-containing compound as well as a preparation method and application thereof. The glycoside group-containing compound has a structure as shown in a formula (1). The polymer obtained by polymerizing the compound can improve the water-drive recovery efficiency of conventional oil reservoirs and common heavy oil reservoirs. # imgabs0 #

This invention provides a halogenated compound for 5' end capping of nucleic acids and its applications, the compound being shown in formula (I). This invention also provides the uses and effects of the above-described compound on nucleic acid transcription and expression.

Among other things, the present disclosure provides oligonucleotide compositions and methods thereof. In some embodiments, the present disclosure provides structural elements including various chemical modifications of sugars, nucleobases and / or internucleotidic linkages that are useful for adenosine editing. In some embodiments, the present disclosure provides methods for preventing or treating conditions, disorders or diseases that can benefit from editing of an adenosine in a nucleic acid.

The invention provides a preparation method and application of salidroside. The preparation method comprises the following steps: culturing an engineering strain for synthesizing the salidroside by using a culture medium containing a mixed carbon source to obtain the salidroside, the mixed carbon source comprises glucose and glycerol. The problem that a salidroside preparation method in the prior art is not suitable for industrial large-scale production can be solved, and the method is suitable for the technical field of biosynthesis.

The invention discloses sesquiterpenoids as well as a preparation method and application thereof. The structure of the sesquiterpenoids is shown in the specification. The sesquiterpenoids of # imgabs0 can be used for preparing drugs for preventing and / or treating cancers, and the cancers comprise at least one of breast cancer, liver cancer, lung cancer, colorectal cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, cervical cancer, kidney cancer and skin cancer. According to the invention, various natural product extracts are subjected to activity screening, the sesquiterpenoids derived from the rattan chrysanthemum have excellent inhibitory activity on various different types of human cancer cells, and an optional scheme is provided for broad-spectrum anti-cancer drugs. The sesquiterpenoids applied in the invention are derived from rattan chrysanthemum, the source is wide, and the production cost is low.

Among other things, the present disclosure provides designed DMD oligonucleotides, compositions, and methods of use thereof. In some embodiments, the present disclosure provides technologies useful for repairing mutant DMD transcripts by skipping exon 51 or exon 53, so that the transcript can be translated into an internally truncated but at least partially functional Dystrophin protein variant. In some embodiments, the present disclosure provides technologies useful for modulating DMD transcript splicing. In some embodiments, provided technologies can alter splicing of a dystrophin (DMD) DMD transcript. In some embodiments, the present disclosure provides methods for treating diseases, such as muscular dystrophy, including but not limited to Duchenne muscular dystrophy, Becker's muscular dystrophy, etc.

Among other things, the present disclosure provides oligonucleotide compositions and methods thereof. In some embodiments, oligonucleotides comprise various chemical modifications. In some embodiments, the present disclosure provides technologies useful for manufacturing oligonucleotides.

The invention belongs to the technical field of biology, and particularly relates to an extraction method of polydeoxyribonucleotide, which comprises the following steps: carrying out homogenate treatment on pretreated in-vitro testis tissue, filtering the obtained homogenate, adding a pancreas extract into the filtrate, and stirring to obtain enzymatic hydrolysate; and precipitating protein in the enzymatic hydrolysate, removing the protein, adding sodium acetate for treatment, adding absolute ethyl alcohol for precipitation, centrifuging, washing the obtained precipitate, and drying to obtain the product. Common trypsin and lipase in fish pancreas are beneficial to release of DNA, the fish pancreas extract is applied to extraction of the polydeoxyribonucleotide, the extraction time can be remarkably shortened, the yield can be increased, meanwhile, nuclease in the fish pancreas extract can effectively degrade DNA, and small-fragment polydeoxyribonucleotide is obtained.

The invention provides an adenosine dihydroxysuccinate eutectic crystal and a preparation method and application thereof.In the adenosine dihydroxysuccinate eutectic crystal, the molar ratio of a dihydroxysuccinic acid molecule to an adenosine molecule is 1: (1-4), on the premise that the structure of adenosine is not changed, dihydroxysuccinic acid and adenosine are combined, the water solubility of adenosine is improved, and therefore the adenosine dihydroxysuccinate eutectic crystal is obtained. According to the preparation method, the adenosine which is difficult to dissolve in water is prepared into the water-soluble adenosine dihydroxysuccinate eutectic crystal, so that the water solubility of the adenosine dihydroxysuccinate eutectic crystal can reach 4%, the effects of the adenosine dihydroxysuccinate and the adenosine monomer are not damaged, and the adenosine dihydroxysuccinate and the adenosine monomer have a better synergistic effect; compared with a monomer and a simple mixture, the composition has better effects in the aspects of antibiosis, antioxidation, anti-aging, hair care, hair loss prevention and the like, and has a good application prospect in skin and hair care products.

The invention discloses a method for preparing sophorolipid by using candida mycoderma bacteria. The method comprises the following steps: inoculating a seed solution of candida mycoderma bacteria into a fermentation culture medium for fermentation, supplementing materials and the like. According to the method, a lactone-type-based product can be obtained, a high-purity product can be further obtained through a two-stage extraction process, alkali liquor is added according to downstream requirements subsequently for blending, and liquid products with different acid type proportions are obtained and used in the field of home care; in addition, the bacterium-containing wastewater obtained through separation can be subjected to spray drying to obtain a powdery solid, and the powdery solid can be used as a low-end product in the agrochemical direction.

The invention belongs to the technical field of organic chemical synthesis, and particularly relates to a gentiopicroside amide derivative, a synthesis method and application. A series of gentiopicroside amide derivatives are synthesized by taking gentiopicroside as a research object, carrying out structural modification on 6 '-site hydroxyl of gentiopicroside and introducing a benzoyl chloride group into a gentiopicroside structure. The synthetic method has the beneficial effects that on one hand, the synthetic method of the gentiopicroside amide derivative, which is mild in reaction condition and environment-friendly, is established; on the other hand, the 20 synthesized gentiopicroside amide derivatives have a remarkable inhibition effect on expression of LPS-induced THP-1 macrophage IL-6 and STAT3 protein content, the anti-inflammatory activity is remarkable, the action effect is superior to that of parent compound gentiopicroside, and a new thought is provided for preparation of STAT3 small-molecule inhibitors and anti-inflammatory drugs.

The invention belongs to the field of biological fermentation synthetic medicines, and particularly relates to tocopherol glucoside and a preparation process thereof. The synthesis method comprises the following steps: (1) preparing an enzyme catalyst; (2) carrying out enzymatic conversion reaction; (3) purifying ion resin; (4) removing impurities; the synthesis process provided by the invention is simple in route and strong in operability, the prepared tocopherol glucoside is high in purity, the total yield of the product is greater than 30%, and the purity is greater than 95.0%.

The invention discloses a nitroreductase response fluorescence and magnetic resonance bimodal probe NO2-Cy-Gxtb-Gd-Biotin designed on the basis of a gemcitabine prodrug as well as a synthesis method and application of the nitroreductase response fluorescence and magnetic resonance bimodal probe NO2-Cy-Gxtb-Gd-Biotin. The probe has relatively low fluorescence signals and magnetic resonance signals, and has relatively low biotoxicity. After the nitroreductase responds, on one hand, the gemcitabine anti-cancer drug is released, and tumor cell apoptosis is promoted; on the other hand, a molecular skeleton can generate a series of electron transfer to form an intermediate structure of methylquinone, and the intermediate structure of methylquinone is very high in reaction activity and is easily attacked by an external residue nucleophilic group of protein, so that the probe is anchored on nitroreductase, and a fluorescence signal and a magnetic resonance signal are remarkably enhanced; therefore, tumor enrichment is increased, an imaging time window is expanded, and early monitoring of tumors is facilitated. The medicine and the imaging probe are integrated, so that the treatment of monitoring tumors is facilitated.

The present invention is the use of a small group of purine nucleotide phosphoramidate disclosed herein or a pharmaceutically acceptable salt thereof in an effective amount for the treatment or prevention of the novel 2019 coronavirus disease (COVID-19) in a host, for example a human, in need thereof.

The invention discloses preparation of a monatomic graphene type photo-thermal catalyst and application of the monatomic graphene type photo-thermal catalyst in catalyzing aldose to produce ketose, and the preparation comprises the following steps: loading a monatomic precursor (X) on a graphene type carbon material (GT) by adopting a solution impregnation method, further reducing the monatomic precursor into a corresponding monatomic (Y) by adopting a continuous oxidation-reduction method, the monatomic graphene type photo-thermal catalyst is obtained, and the monatomic graphene type photo-thermal catalyst is expressed as Ysg / GT. The catalyst is used for catalyzing other different aldoses (maltose, glyceraldehyde, erythritol and xylose) to produce corresponding ketose, the catalyst shows good catalytic activity and photo-thermal performance, and the ketose yield is larger than 50%. Finally, in the reusability research of the catalyst, the catalyst is found to have good activity and structural stability, the chemical equilibrium limit of the reversible reaction is broken through, the reaction rate is increased, and the catalyst can help to develop an efficient and green catalyst to open up a new way for the reversible reaction process.

The invention provides a method for synchronously extracting tea seed oil and tea saponin from tea seeds, and belongs to the technical field of active substance extraction. According to the method, the extraction rate and the purity are remarkably improved by finely regulating and controlling the extraction conditions. The specific composite oil extraction auxiliary agent and the ultrasonic-assisted extraction technology are adopted, and the power, time and solvent ratio are optimized, so that the extraction efficiency and the product quality of the tea seed oil and the tea saponin are superior to those of the tea seed oil and the tea saponin prepared by a traditional method. Experimental data prove that the extraction rate of the tea seed oil reaches 27.22%, and the purity of the tea seed oil reaches 99.66%; the extraction rate of the tea saponin is 21.85%, and the purity of the tea saponin reaches 96.50%. By increasing the utilization rate of the raw materials and reducing the production cost, the market competitiveness of the product is improved, the high-quality tea seed oil and tea saponin meet the requirements of the market for natural products, and the tea seed oil and tea saponin have wide application prospects.

The present disclosure, among other things, provides technologies for synthesis, including reagents and methods for stereoselective synthesis. In some embodiments, the present disclosure provides compounds useful as chiral auxiliaries. In some embodiments, the present disclosure provides reagents and methods for oligonucleotide synthesis. In some embodiments, the present disclosure provides reagents and methods for chirally controlled preparation of oligonucleotides. In some embodiments, technologies of the present disclosure are particularly useful for constructing challenging internucleotidic linkages, providing high yields and stereoselectivity.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention discloses an arbutin derivative 6-O-Caffeoylbutin as well as a preparation method and application of the arbutin derivative 6-O-Caffeoylbutin. The structural formula of a 6-O-Caffeoylbutin compound is shown as a formula (I): # imgabs0. The invention also discloses application of the arbutin derivative (6-O-Caffeoylbutin) disclosed by the invention in preparation of an antioxidant preparation. According to the invention, the 6-O-Caffeoylbutin extracted for the first time shows excellent antioxidant activity, the ability of the 6-O-Caffeoylbutin to remove ABTB < + > free radicals is stronger than that of ascorbic acid and beta-arbutin, and a material basis is provided for development of novel antioxidants.

Disclosed are immunologically active saponin fraction composition, its method of manufacture and use in vaccine adjuvants, and in adjuvanted vaccines. Methods of preparation, vaccine and treatment kits comprising the adjuvants, and uses of vaccines and vaccine kits comprising the adjuvants are also disclosed.

The invention discloses a method for preparing cellobiose and comprehensively utilizing a by-product of cellobiose. The method is characterized by specifically comprising the following steps: (1) dilution, (2) enzymolysis, (3) flocculation and carbon explosion, (4) decoloration, (5) continuous ion exchange, (6) evaporation and concentration, (7) simulated moving bed chromatographic separation, (8) concentration, (9) finished product preparation and (10) raffinate utilization. The method effectively solves the problems of low preparation purity and high production cost of cellobiose.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The invention belongs to the field of preparation of medical intermediates, and particularly relates to a preparation method of an On-DNA sulfinic acid structure compound. The method for preparing the On-DNA sulfinic acid structure compound comprises a step of oxidizing On-DNA thiol (phenol) into the On-DNA sulfinic acid structure compound under alkaline and heating or illumination conditions, a solvent used in the reaction step is a mixed solution of water, dimethyl sulfoxide, N, N-dimethylformamide, acetonitrile, ethanol or tetrahydrofuran, the reaction temperature is not more than 80 DEG C, and the reaction time is not more than 2 hours. And the illumination wavelength ranges from 365 nanometers to 535 nanometers. The On-DNA sulfinic acid structure compound obtained in the invention can be directly used for constructing a structure compound with a sulfonyl group, the reaction condition is mild and convenient, a new method and a new idea are provided for construction of a DNA coding compound library, and the On-DNA sulfinic acid structure compound has a very good application prospect in lead drug development.

The invention discloses modified polystyrene resin as well as a preparation method and application thereof, and belongs to the technical field of medicine purification. The modified polystyrene resin contains phenolic hydroxyl groups and sulfonic acid groups at the same time, electrostatic interaction and hydrogen-bond interaction can be generated on pyridine, the two actions are complementary, and pyridine molecules in different protonation states can be captured; moreover, a benzene ring of the modified polystyrene resin can be combined with an aromatic ring of pyridine through pi-pi stacking, and the introduction of a sulfonic acid group and a phenolic hydroxyl group further polarizes an electron cloud of the benzene ring of polystyrene, so that the conjugation effect can be enhanced, and the bonding strength of the benzene ring and the aromatic ring of pyridine is improved. The modified polystyrene resin prepared by the method has nanoscale pores and ultrahigh specific surface area, can form a'pore capture-group immobilization 'synergistic path when pyridine is adsorbed, and can effectively remove residual pyridine in a reaction system.

The invention provides a method for preparing lactulose from immobilized beta-galactosidase, and belongs to the technical field of functional sugar preparation.The method comprises the following steps that an immobilized enzyme column is prepared, specifically, macroporous alkalescent styrene anion exchange resin is soaked and washed with an ethanol solution, a NaCl solution, an HCl solution and a NaOH solution in sequence; the pretreated resin and beta-galactosidase liquid are mixed, a buffer solution is added, shaking table adsorption is performed, and standing is performed after adsorption; adding a glutaraldehyde solution for cross-linking, and discarding the supernatant to obtain an immobilized enzyme column; adding a magnesium ion solution into a mixed solution of lactose and fructose, and then performing conversion through an immobilized enzyme column to obtain a lactulose conversion solution; the lactulose conversion liquid is sequentially subjected to decoloration, ion exchange, vacuum concentration and continuous simulated moving bed chromatographic separation to obtain a lactulose solution, by means of the method, the lactulose conversion rate is larger than or equal to 60%, the lactulose yield is larger than or equal to 92%, the preparation process is simple, and the cost is low.

The invention provides a stationary phase for DNA synthesis and a preparation method and application thereof. The preparation method of the stationary phase for DNA synthesis, provided by the invention, comprises the following steps: S1, preparing nano magnetic ferroferric oxide particles, and coating the surfaces of the nano magnetic ferroferric oxide particles with SiO2 layers to obtain a nano magnetic composite material Fe3O4-SiO2; s2, carrying out azide group modification on the surface of the nano magnetic composite material Fe3O4 (at) SiO2 to form a surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3; and S3, loading the initiator to the surface of the surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3 through Click reaction to obtain the stationary phase for DNA synthesis. The stationary phase provided by the invention can be used for DNA synthesis, and has the characteristics of high bonding strength, good stability, difficult shedding of initiators, high DNA synthesis yield and the like.