96 results about "Viral disease" patented technology

The invention discloses a bovine I-type alpha interferon-ferritin fusion protein, a mutant thereof, a preparation method and an application of the bovine I-type alpha interferon-ferritin fusion protein. The bovine I-type alpha interferon is fused with a ferritin subunit, and interferon molecules are highly repeatedly and orderly displayed on the surface of a ferritin nanocage by utilizing the self-assembly characteristic of ferritin, so that the expression level, the structural stability and the antiviral activity of the interferon are remarkably improved. The fusion protein is further subjected to single-site or multi-site rational design mutation, and a mutant with significantly improved antiviral activity and stability is obtained. According to the invention, a silkworm or insect cell eukaryotic expression system is adopted to express the fusion protein or the mutant thereof, and the expression system is safe to operate, simple and convenient in procedure, low in cost and extremely beneficial to large-scale industrial production; the prepared fusion protein or mutant nanoparticles have application prospects in preparation of drugs or reagents for preventing or treating bovine viral diseases.

The present invention belongs to the technical field of biological prevention and treatment for viral diseases. Disclosed in the present invention are a tomato disease-resistant gene mutant, and the use thereof in the prevention and treatment of ToBRFV. It is found in the present invention that mutating the nucleotide at position 1927 of the coding region sequence of tomato Tm-22 gene from G to A, or mutating the amino acid at position 643 of the LRR domain of a protein that is encoded by tomato Tm-22 gene from glycine to arginine can remarkably reduce the accumulation level of ToBRFV capsid protein (CP). The gene (named Tm-22-Mut5) can serve as a novel ToBRFV resistant gene, and also retains the resistance to TMV, ToMV and ToMMV. The Tm-22-Mut5 and a pre-screened Tm-22-Mut3-1 mutant (tyrosine at position 767 of the LRR domain thereof is mutated to phenylalanine) undergo combinatorial mutagenesis to obtain a mutant Tm-22-Mut6. Analysis shows that the Tm-22-Mut6 can remarkably reduce the accumulation level of the ToBRFV capsid protein. Compared with the pFGCTm-22-Mut3-1 mutant obtained by screening in the previous research and the newly obtained mutant Tm-22-Mut5, the Tm-22-Mut6 has further improved resistance to ToBRFV.

A fusion protein comprising an anti-PD1 antibody moiety having a C-terminus, and an IL21 mutant protein conjugated to the anti-PD1 antibody moiety having a C-terminus wherein the IL21 mutant protein comprises at least one of two mutations relative to wild-type IL21: (1) one of a K72Y substitution, a K72M substitution and a K72Q substitution, and (2) a 75-80 deletion. In some examples, the anti-PD1 antibody may take the ETYY format and may comprise: a first heavy chain comprising a VH comprising the sequence of SEQ ID NO: 11; and a second heavy chain comprising a VH comprising a sequence selected from the sequences of SEQ ID NO: 8-10. Also provided are pharmaceutical compositions comprising the fusion protein, nucleic acids encoding the fusion protein, vectors comprising the nucleic acids, and methods of administering the fusion protein to treat diseases.

This application relates to pyrimidine derivatives described in formula (I-1), formula (I-2), formula (II-1), or formula (II-2), or their stereoisomers, tautomers, isotopic derivatives, hydrates, solvates, prodrugs, and pharmaceutically acceptable salts, which can be used as effective TLR7 and / or TLR8 receptor agonists and are characterized by good selectivity, high activity, and excellent safety. This application also provides a method for preparing the pyrimidine derivatives and a pharmaceutical composition thereof. The compounds of this application or their pharmaceutical compositions can be used in the preparation of pharmaceuticals for the prevention or treatment of infectious diseases, respiratory diseases, immune-related diseases, viral diseases, or tumors. TIFF2026528992000173.tif3891

The invention relates to the technical field of plant growth regulation, in particular to application of berberine based on ACAA targets in resisting plant viruses and promoting plant growth. The invention reveals that berberine plays a role by specifically targeting acetyl coenzyme A acyltransferase and up-regulating the expression of the acetyl coenzyme A acyltransferase for the first time. The clear compound-target-pathway mechanism overcomes the defect that the existing plant source product mechanism is unknown. As the target spot is clear, the application dosage can be accurately controlled, so that the stability and predictability of field antiviral and growth promoting effects are ensured, and a foundation is laid for large-scale agricultural application. An ACAA target is activated by berberine, and an antiviral defense mechanism and an energy metabolism growth pathway of a plant can be synchronously started, so that the plant growth is effectively promoted while virus diseases are prevented and treated. The disease resistance-growth negative correlation problem existing in agricultural production for a long time is solved, and the dual requirements of modern agriculture for precise prevention and control and yield increase and quality improvement are met.

The invention discloses bacillus subtilis subsp.deserticola, nano-selenium synthesis application of the bacillus subtilis subsp.deserticola and application of the bacillus subtilis subsp.deserticola to prevention and treatment of plant virus diseases, and belongs to the technical field of microorganisms. The bacillus subtilis subsp. Deserticola KBD-4 has better selenium tolerance and can reduce selenium salt into nano-selenium, and meanwhile, a bacterial solution and a nano-selenium active bacterial solution which are generated after the strain is fermented have a better inhibition effect on plant viruses such as TMV, CMV, PVY and TSWV, so that the plant virus diseases can be better prevented and treated. Besides, a biological source nano-selenium potassium fertilizer can be prepared by adding a high-potassium trace element additive into the nano-selenium active bacterial liquid, after root irrigation, the disease-resistant and growth-promoting effects on the tomatoes are better, the tomato yield is increased, the nutritional quality of the tomatoes is further improved, selenium-rich agricultural products meeting the national standard are produced, and the method is worthy of popularization and application. The method has good practical application value in production.

The invention discloses a pH-responsive isoGPBG hydrogel and an application of the pH-responsive isoGPBG hydrogel in miRNA delivery, and belongs to the technical field of biopesticides. The pH-responsive isoGPBG hydrogel disclosed by the invention is formed by deprotonating isoguanosine and guanosine under an alkaline condition and cross-linking the deprotonated isoguanosine and guanosine with a boric acid group of PBA to form a pH-sensitive phenylboronic acid ester bond. The hydrogel delivery system can break through plant miRNA transmembrane transport obstacles, and efficient delivery and controllable release of antiviral nucleic acid drugs are achieved. When the hydrogel delivery system is used for delivering miRNA, in-vitro degradation of miRNA can be prevented, and the hydrogel delivery system has an important application prospect in prevention and treatment of plant viral diseases and bacterial diseases.

The invention discloses preparation and application of furoic acid and a bactericidal composition thereof, and relates to the technical field of biopesticides, the furoic acid comprises furoic acid, the chemical structural formula of the furoic acid is shown in the specification, the purity is greater than or equal to 98%, and the furoic acid is prepared by the following steps: mixing furfural, a sodium hydroxide aqueous solution and copper oxide, introducing oxygen at 50-60 DEG C, stirring and reacting for 4-6 hours, and filtering to obtain a filtrate; extracting, acidifying, recrystallizing and drying to obtain furoic acid; according to the preparation and application of the furoic acid and the bactericidal composition thereof, the furoic acid with a specific structure is scientifically compounded with screened specific biopesticides (kasugamycin, boscalid and ningnanmycin), and the ratio range (for example, the ratio of furoic acid to kasugamycin is 1: 10-10: 1) with a remarkable synergistic effect is optimized and determined; according to the composition disclosed by the invention, the components are effectively complemented on the action mechanism, and the defects that the traditional single pesticide is single in action target and easy to induce drug resistance are overcome, so that the overall control effect of the composition on various plant diseases (including bacteria, fungi and virus diseases) is remarkably improved.

Disclosed herein are compositions and methods for improving pancreatitis through pharmacological manipulation of calcium signaling. Such compositions and methods can be used to improve symptoms of acute or chronic pancreatitis or to reduce the likelihood or severity of pancreatitis in individuals at risk for the condition. In other embodiments, disclosed herein are compositions and methods for improving viral disease through pharmacological manipulation of calcium signaling. In further embodiments, disclosed herein are compositions and methods for improving Thl7-induced disease through pharmacological manipulation of calcium signaling.

The invention relates to a compound preparation for preventing and treating tobacco mosaic disease and application thereof. Aiming at the problems that chemical control easily causes pesticide residues, biological control takes effect slowly and is unstable, the threshold of a gene editing technology is high and the like in the existing tobacco mosaic disease control technology, the invention provides a medicament compounded by azelaic acid and beta-aminobutyric acid according to a molar ratio of 1: (2-10). According to the agent, antioxidant enzyme systems such as SOD (superoxide dismutase), CAT (catalase) and POD (peroxidase) in tobacco plants are synergistically activated, and meanwhile, SAR (synthetic aperture radar) and SA / ABA double-signal channels are triggered, so that the self-defense capability of the plants is Experiments show that the SOD activity is improved by more than 40% after transplanting for 30 days after treatment by the medicament, and the CAT activity is improved by more than 125% after 60 days, so that the morbidity of the tobacco mosaic disease is obviously reduced, the disease index in 30 days is lower than 1.0, and the morbidity in 60 days is reduced by more than 10% compared with that of a control group. The method is easy and convenient to operate, environmentally friendly and free of residual risk, achieves the dual effects of early-stage rapid inhibition and long-term continuous control, and is efficient and safe in tobacco virus disease prevention and treatment.

The application provides a kind of 3, 4-dichloroisothiazole benzamide derivatives and preparation method and purposes thereof, specifically relates to a kind of derivatives, and the chemical structure general formula is seen formula I: the application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, acaricide, fungicide, anti-plant virus agent, plant activator purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, acaricide, fungicide, anti-plant virus agent, plant immune activator in the prevention and treatment of agricultural, forestry, horticultural plant pest, mite damage, disease, viral disease purposes and preparation method.

The present invention relates to a fusion protein comprising an anti-PD-1 antibody moiety (having a C-terminus) and an IL-21 variant bound to the C-terminus of the anti-PD-1 antibody moiety. The IL-21 variant contains at least one of the following mutations relative to wild-type IL-21: (1) any of K72Y substitution, K72M substitution, or K72Q substitution, and (2) a 75-80 amino acid deletion. For example, the anti-PD-1 antibody may employ an ETYY format, with the first heavy chain containing the VH sequence shown in SEQ ID NO: 11, and the second heavy chain having a VH containing any of the sequences in SEQ ID NOs: 8-10. Furthermore, the present invention also provides a pharmaceutical composition containing the fusion protein, a nucleic acid encoding the fusion protein, a vector containing the nucleic acid, and a method for treating a disease using the fusion protein.

The present invention relates to pharmaceutically acceptable co-crystals and salts of 8-chloro-N-(4-(trifluoromethoxy)phenyl)quinolin-2-amine, to pharmaceutical compositions comprising them, and to their use as medicaments, and more particularly for use in the prevention and / or treatment of inflammatory diseases, viral diseases, and / or cancer or dysplasia. The present invention also relates to methods for preparing such pharmaceutically acceptable co-crystals and salts.

The invention belongs to the technical field of disease prevention and control in aquaculture, and particularly relates to a fluorescent aptamer sensor capable of specifically recognizing white spot syndrome virus as well as a preparation method and application of the fluorescent aptamer sensor. The fluorescent aptamer sensor is composed of a nucleic acid aptamer capable of specifically recognizing the white spot syndrome virus and an aggregation-induced emission material, and the sequence of the nucleic acid aptamer capable of specifically recognizing the white spot syndrome virus is as shown in SEQ ID NO. 1. The fluorescent aptamer sensor realizes specific recognition of the white spot syndrome virus, is high in sensitivity, rapid and efficient in detection, accurate and reliable in result and low in cost, and remarkably improves the diagnosis efficiency of viral diseases in aquaculture.

The application discloses a bovine type I alpha interferon-ferritin fusion protein, a mutant thereof, and a preparation method and application thereof. The bovine type I alpha interferon is fused with a ferritin subunit, the self-assembly characteristics of the ferritin are utilized, the interferon molecules are highly repeated and orderly displayed on the surface of the ferritin nanocage, and the expression level, structural stability and antiviral activity of the interferon are significantly improved. The fusion protein is further subjected to rational design mutation at a unit point or multiple points, and a mutant with significantly improved antiviral activity and stability is obtained. The fusion protein or the mutant thereof is expressed by using a silkworm or insect cell eukaryotic expression system, the expression system is safe in operation, simple in procedure, low in cost, and extremely beneficial to large-scale industrial production, and the prepared fusion protein or mutant nanometer particle has application prospects in the preparation of medicines or reagents for preventing or treating bovine viral diseases.

This invention belongs to the field of aquaculture technology and relates to a novel use of capsaicin, particularly its use in the preparation of drugs and / or feed additives for the prevention and treatment of fish viral infections. The core of this invention lies in the significant protective effect of capsaicin against carp spring viremia virus (SVCV), grass carp reovirus (GCRV), and carp herpesvirus type 2 (CyHV-2) infections, effectively inhibiting the transcription of SVCV, GCRV, and CyHV-2 viral genes and the expression of viral proteins. Feed additives and / or drugs containing capsaicin can be used for the prevention and treatment of fish viral diseases, offering advantages such as high protective efficiency and environmental friendliness.

The invention discloses lactobacillus mucosa with porcine virus resisting activity and application of the lactobacillus mucosa, and relates to the technical field of microorganism application and virus prevention and control. It is found for the first time that the lactobacillus mucosa has broad-spectrum antiviral activity, not only has a remarkable inhibition effect on porcine reproductive and respiratory syndrome virus type 2 (PRRSV2) strains, but also can further resist porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV); according to the present invention, the Lactobacillus mucosa is adopted to culture the supernatant, such that the supernatant does not have toxicity on Marc-145 cells; according to the PRRSV2 strain treated by using the supernatant, the expression level of virus RNA and protein and the filial generation virus titer are remarkably reduced, so that the infection ability is weakened; the culture supernatant directly acts with virus particles to block infection and replication of the virus particles; the product disclosed by the invention is derived from natural probiotics, is high in safety, environment-friendly and not easy to resist drugs, can be used for preparing a broad-spectrum anti-porcine virus biological preparation, provides a new strategy for preventing and controlling porcine virus diseases, and has a good industrial application prospect.

This invention discloses a green control method for tomato viral diseases based on the synergistic effect of Solanaceae and Asteraceae weeds, belonging to the field of agricultural plant crop protection technology. This invention uses the Solanaceae weed *Solanum nigrum* and the Asteraceae weed *Chrysanthemum indicum* as indicator plants for tomato viral diseases. By arranging the indicator plants in a grid pattern, comprehensive monitoring of major viral disease species within the facility can be achieved. By monitoring the viral disease symptoms of the indicator plants, early warnings can be issued 7-10 days in advance, allowing more time for tomato disease control and improving the timeliness and proactivity of prevention and control. Furthermore, based on the warnings issued by the indicator plants, an extract of *Eupatorium fortunei*, an Asteraceae weed that has a significant inhibitory effect on tomato viral disease symptoms, is applied, thus constructing an integrated "monitoring-blocking" green control system, achieving early warning and efficient control of viral diseases.

The invention belongs to the field of biological medicine, and particularly relates to novel application of a functional regulator of xylosyltransferase 1 (XYLT1) in preparation of drugs for resisting human immunodeficiency virus (HIV) infection and / or resisting tumor immunotherapy. In-vitro experiments prove that up-regulation of expression or activity of XYLT1 can inhibit HIV virus replication (such as reduction of p24 antigen and virus infectivity) and enhance the curative effect of a broad-spectrum neutralizing antibody (such as 3BNC117); meanwhile, the XYLT1 can mediate the degradation of PD-L1 protein, relieve the inhibition on T cells and enhance the anti-tumor killing activity of CD8T cells. The invention provides a new target spot and a combined treatment strategy for immunotherapy of viral diseases and tumors.

The present application provides a kind of containing isochromene pyridone derivatives and its preparation method and purposes, specifically related to a kind of derivatives, its chemical structure general formula see formula I: The present application discloses the general formula of the structure of the above compound, synthesis method and as insecticide, fungicide, anti-plant virus agent purposes, it is combined with agricultural acceptable adjuvant or synergist and with commercial insecticide, fungicide, anti-plant virus agent, miticide in the prevention and treatment of agricultural, forestry, horticultural plant pest, disease, viral disease purposes and preparation method.

The present invention relates to phenyl amino pyrimidine compounds which are inhibitors of protein kinases including JAK kinases. In particular the compounds are selective for JAK2 kinases. The kinase inhibitors can be used in the treatment of kinase associated diseases such as immunological and inflammatory diseases including organ transplants; hyperproliferative diseases including cancer and myeloproliferative diseases; viral diseases; metabolic diseases; and vascular diseases.

Pharmaceutical compositions of the invention comprise functionalized benzamide derivatives useful as cyclic GMP-AMP synthase-Stimulator of interferon gene (cGAS-STING) pathway agonists, and useful for treating viral diseases and boost antitumor immunity.

The invention is directed to methods and systems for early detection of viral diseases, and more specifically to systems and methods for early detection of viral diseases that are capable of detecting very low viral loads, such as for example and not limitation, SARS-CoV-2 loads.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention provides a compound containing a general formula I as well as a preparation method, a pharmaceutical composition and application thereof. Specifically, the invention provides a compound with a structure as shown in a general formula I, and a raceme, an R-isomer, an S-isomer, pharmaceutically acceptable salt or a mixture thereof. The compound has good inhibitory activity on the PL protease, can be used for treating, preventing and relieving diseases related to the PL protease, and particularly can be used for treating viral diseases with the PL protease, such as viral diseases caused by SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-OC43 and the like.

The invention discloses a virus disease resistance gene and application of excellent natural variation of the virus disease resistance gene, and belongs to the technical field of biological breeding and vegetable production. The anti-ToCV function of the SlAVE3 gene is identified for the first time, the antiviral mechanism of the SlAVE3 gene for targeted degradation of virus p59 protein through a UPS system and regulation and control of transcription positive feedback circulation is clarified, and the blank of tomato ToCV resistance gene identification is filled. The invention provides a mutation form of the SlAVE3, clarifies the influence of key site mutation on the function of the SlAVE3, and provides a target for optimizing the antiviral activity of the SlAVE3 through a gene editing technology. The excellent haplotype SpAVE3 derived from wild tomatoes is explored, the ToCV resistance of the excellent haplotype SpAVE3 is obviously superior to that of SlAVE3 of cultivated tomatoes, and a precious gene resource is provided for disease-resistant breeding of tomatoes.

The present invention provides di(dispirodiazatripiperazine)-triazine compounds and / or pharmaceutical compositions, such compounds and / or compositions for use in methods of treating or preventing diseases, in particular, viral diseases or infections, and methods for manufacturing such compounds and compositions.

A method for reducing mortality resulting from viral infection, reducing viral load resulting from viral infection, or increasing immunity to viral infection in a subject is described. The method comprises administering a hybrid protein comprises a first domain comprising a sequence encoding a surface protein of an enveloped RNA virus and a second domain comprising a sequence encoding an ectodomain of a type 2 transmembrane domain protein, wherein the second domain is located at the C-terminal of the first domain. The hybrid protein or an mRNA encoding such protein can be used as a vaccine against the infection of the enveloped RNA virus.

The present invention provides a method for treating, reducing the incidence of, suppressing or inhibiting a viral infection, disease, disorder or symptom thereof in a subject in need thereof, comprising administering to the subject a selective SIR agonist. In another aspect, the viral disease is COVID-19 and the selective SIR agonist is pridopidine or a pharmaceutically acceptable salt thereof.