31 results about "Normal blood" patented technology

The application discloses a kind of thrombus suction control method and thrombus suction device, and thrombus suction device includes suction assembly, suction assembly includes the shell with pressure cavity, piston rod and suction catheter, thrombus suction device also includes the motor of piston rod movement and the controller connected with motor, control method is: suction catheter is positioned to the blood vessel without thrombus, make motor operate according to speed V0, calibrate the pressure value B in pressure cavity under normal blood condition;Suction catheter is positioned to the blood vessel with thrombus, make motor operate according to speed V1, V1≤V0, make dynamic pressure value A0 in pressure cavity: when A0≤B, make motor keep speed V1 operation;When A0>B, make motor increase from speed V1: when B<A0≤C, make motor increase to speed V2 operation;When C<A0≤D, make motor increase to speed V3 operation;When D<A0≤E, make motor increase to speed V4 operation.The control method thrombus removal efficiency is high, and blood loss can be reduced.

The invention relates to the technical field of underground safety monitoring and occupational health protection, and discloses an eye movement and multi-mode sensing underground protective glasses and an early warning method. And an elevation angle eye movement sensing module, an inertial sensor, a PPG and body temperature galvanic skin sensor, an acoustic component, a wireless communication module and a detachable glasses leg battery module are connected. The method comprises the steps that eye images and multi-source physiological data are collected in real time, and a multi-dimensional state judgment model is constructed; the logic contradiction between normal blood oxygen value and slow eye movement response is innovatively used for recognizing hidden carbon monoxide poisoning, accidental slipping and coma falling are distinguished through a posture and eye movement cooperation mechanism, and meanwhile the active noise reduction function is achieved. The problems that traditional equipment cannot recognize hidden poisoning and the falling false alarm rate is high are solved, and accurate sensing and early warning of physiological fatigue, poisoning symptoms and safety states of underground personnel are achieved.

According to the bleeding cage and the blood pump thereof, the blood mixing loss can be reduced, the supporting strength is good, the whole bleeding cage is in a circular tube shape, a far-end opening of the bleeding cage forms a blood inlet, a bleeding opening and a flushing outlet are formed in the outer wall of the bleeding cage, and the bleeding opening and the flushing outlet are formed in the axial direction at intervals. Normal blood pumped by the blood pump flows out from the blood outlet, and flushing fluid flows out from the flushing outlet after flushing the gap, so that energy loss caused by blood mixing is reduced, and the hydraulic performance of the blood pump is improved; the bleeding cage has a certain length in the axial direction, and the bleeding opening and the flushing outlet are arranged at an interval, so that the overall structural strength of the bleeding cage is ensured, and the bleeding cage is prevented from being deformed due to stress; meanwhile, the turbulence influence generated when blood flows through the bleeding cage is reduced, stable rotation of the magnet is improved, and then the rotation stability of the whole blood pump is guaranteed.

The utility model relates to the technical field of vein blood sampling, in particular to an anti-backflow vein blood sampling device. The utility model provides an anti-backflow vein blood sampling device which can effectively prevent blood from flowing back and ensure normal blood sampling work. An anti-backflow vein blood sampling device comprises a needle head, a connecting piece, a needle wing, a connecting pipe, a fixing assembly and the like, the connecting piece is connected to the left portion of the needle head, the needle wing is connected to the upper side of the right portion of the connecting piece, the connecting pipe is connected to the left side of the connecting piece, and the fixing assembly capable of being fixed to an arm is arranged on the connecting piece. After blood flows into the control cylinder, the blood flows into the conical thin film and the hard tube, the conical thin film and the hard tube are kept in linear communication and then flow out of the collecting head, and after the blood stops flowing into the conical thin film, the hard tube droops under the action of gravity to block the conical thin film, so that the blood can be effectively prevented from flowing back; and the effect of normal blood sampling work is ensured.

The invention relates to the technical field of hemostatic materials, and provides chitosan-based in-situ self-gel powder as well as a preparation method and application thereof. The chitosan-based in-situ self-gel powder provided by the invention comprises modified chitosan-based powder and oxidized polysaccharide powder, the modified chitosan-based powder is prepared from poly (3-sulfopropyl methacrylate potassium salt) grafted chitosan and poly [2-(methylacryloyloxy)-ethyltrimethyl ammonium chloride] grafted chitosan. The chitosan-based in-situ self-gelling powder provided by the invention has an in-situ self-gelling function, can quickly absorb water to realize self-gelling under the triggering of body fluid or blood, has excellent wet tissue adhesion performance, is high in water absorption speed and good in hemostatic effect, is not influenced by a normal blood coagulation mechanism in a hemostatic process, and has good postoperative anti-adhesion performance; no chemical cross-linking agent is used in the preparation process of the chitosan-based in-situ self-gel powder, the chitosan-based in-situ self-gel powder has good biocompatibility and biodegradability, raw materials are easy to obtain, synthesis is simple, and the chitosan-based in-situ self-gel powder is economical and safe.

Provided are a branch artery reconstruction assist device and an SC branch-preferential aortic reconstruction system, the branch artery reconstruction assist device comprising a first stent body (20a), the first stent body (20a) comprising a bare stent portion (23a), and a cardiac proximal covered stent portion (21a) and a cardiac distal covered stent portion (22a) connected to both sides of the bare stent portion (23a), and a sealing membrane (219a, 229a) and at least one sealing membrane (229a) are provided inside both the cardiac proximal covered stent portion (21a) and the cardiac distal covered stent portion (22a). Two coated stents (211a, 212a, 221a) are provided, and the sealing membranes (219a, 229a) include first openings (213, 214, 222), which are connected to corresponding first ends of the coated stents (211a, 212a, 221a), and the second ends of the coated stents (211a, 212a, 221a) are connected to corresponding branch artery coated stents (216, 217, 218) to form branch channels, and pass through the bare stent portion (23a). The SC branch-priority aortic reconstruction system includes a branch artery reconstruction assist device and a second stent body (50a), and the second stent body (50a) and the first stent body (20a) cooperate to seal and isolate the lesion. This prevents end leaks from the stent, ensures smooth access to each artery, and ensures normal blood flow to the brain and / or organs during surgery, avoids blood flow blockage, and reduces ischemic complications.

The present invention provides non-human animals expressing human CFB protein or chimeric (e.g., humanized) CFB protein and methods for using the same. The present invention further provides a non-human animal genome, a humanized CFB gene, and cells, tissues, organs, or non-human animals containing the non-human animal genome and the humanized CFB gene. The non-human animals obtained in this application successfully express human CFB protein or humanized CFB protein, which can be cleaved by CFD, bind to C3b, and exert similar effects in the human body. Furthermore, the non-human animals obtained in this application have complete renal function, normal blood biochemical indicators, and, more remarkably, do not exhibit the potential diseases observed in other transgenic mice known in this art.

The present application relates to the technical field of platelet aggregation detection, and specifically discloses a clinical platelet aggregation detection carrier, which comprises an instrument body and further comprises: a detection assembly arranged inside the instrument body, which is used for detecting different types of samples of lipemia, anemia and normal blood and selecting centrifugal operation of the blood samples; the detection assembly can detect different types of samples of lipemia, anemia and normal blood, and can automatically indicate whether centrifugal operation of the blood is needed according to the types of the detected samples; through the sampling assembly, the blood samples at different layer depths can be automatically adjusted according to the lipemia, anemia and normal blood samples detected by the detection assembly; through the agent adding assembly, the amount of the added inducing agent can be automatically adjusted according to the sample amount taken by the sampling assembly, so that the concentration of the mixed inducing agent reaches the specified range, thereby improving the detection accuracy of the platelet aggregation degree.

The application relates to the technical field of medicines, and discloses application of a traditional Chinese medicine composition in serum neurotransmitters, which comprises the following steps: selecting 6-8-week-old mice to establish normal models, chronic fatigue syndrome models and functional dyspepsia models; blood sampling is conducted on the mice of the normal models, the chronic fatigue syndrome models and the functional dyspepsia models to obtain normal blood samples, CFS blood samples and FD blood samples respectively; protein precipitation operation is conducted to obtain normal deproteinized supernatants, CFS deproteinized supernatants and FD deproteinized supernatants; separation is conducted by using a reversed-phase chromatographic column, and then mass spectrometry is conducted to obtain normal serum neurotransmitter mass spectra, CFS serum neurotransmitter mass spectra and FD serum neurotransmitter mass spectra respectively; concentration value calculation and rhythm fluctuation analysis are conducted to obtain a neurotransmitter function index and rhythm stability quantitative score respectively. Therefore, the mechanism of the traditional Chinese medicine composition in serum neurotransmitters is clearly quantitatively characterized.

A method for measuring a titer of a coagulation factor inhibitor. The-method includes: preparing a mixed specimen containing a-subject blood specimen and a normal blood specimen; heating the mixed specimen and acquiring a coagulation reaction-curve for the heated mixed specimen; acquiring a-coagulation reaction curve for the mixed specimen that has-not been heated; calculating a parameter related to the-coagulation reaction curve of the mixed specimen that has-not been heated as a first parameter; calculating a-parameter related to the coagulation reaction curve of the-heated mixed specimen as a second parameter; and-calculating a titer of a coagulation factor inhibitor in-the subject blood specimen on the basis of the ratio or the difference between the first parameter and the second-parameter.

The present application provides a kind of hemodialysis internal fistula nursing hemostasis device, comprising: adjusting box, adjusting box is fixedly installed with bandage;Lifting assembly, lifting assembly includes sleeve, screw and threaded sleeve, sleeve is rotatably arranged in adjusting box, screw is threadedly arranged in the small hole diameter inside of sleeve, threaded sleeve is threadedly arranged in the large hole diameter inside of sleeve, rotating sleeve can adjust screw and threaded sleeve move up and down, and the moving distance of screw is less than the moving distance of threaded sleeve;Pressing assembly, pressing assembly includes pressing block and pressing ring, pressing block and pressing ring are respectively fixedly installed in the lower end of screw and threaded sleeve, and pressing block is located in the inside of pressing ring.It can solve the technical problems that the pressure applied by the existing internal fistula hemostasis device is usually uniformly distributed in the covered area of pressing plate, which easily leads to the long-term unnecessary excessive compression of the normal blood vessel wall around the puncture point, significantly hinders the internal fistula blood flow, and increases the risk of thrombosis and internal fistula acute occlusion.

The invention provides a controllable cerebral blood flow adjusting device for cerebral blood flow reconstruction surgery, and relates to the technical field of medical instruments, the controllable cerebral blood flow adjusting device comprises a connecting elastic piece, and the two ends of the connecting elastic piece are connected with clamping pieces; the clamping piece is of an arc-shaped plate structure, and a traction piece is mounted at the bottom of the clamping piece; the connecting elastic piece, the clamping piece and the traction piece are made of polylactic acid-glycolic acid copolymer. When the device is used, under the assistance of ultrasonic waves, a traction sheet is used on a blood vessel to set proper clamping force so as to artificially cause blood vessel constriction, the inner-layer clamping sheet with different polylactic acid-glycolic acid copolymer proportions is used, the degradation speed of the whole device can be controlled and adjusted, then along with ordered degradation of the device, the clamping force on the blood vessel is gradually reduced, and the blood vessel constriction effect is improved. The constriction degree of the blood vessel is gradually reduced, and the blood vessel is finally recovered to a normal blood vessel diameter, so that the problems of headache, dizziness and other discomfort of a patient caused by intracranial blood flow over-perfusion are solved.

A sample slide preparation device and method are disclosed, wherein the sample transport device transports samples including trace blood samples and normal blood samples. A trace blood sample mixing device drives the movement of the trace blood sample to mix it. A normal blood sample mixing device drives the movement of the normal blood sample to mix it. A control unit determines whether the sample slide preparation device operates in normal blood mode or trace blood mode based on sample type information, and selects different methods to complete sample mixing, sample collection, and movement according to the different modes, finally preparing a sample slide for subsequent detection and analysis.

The invention relates to the technical field of medical instruments, in particular to a vascular intima stripping surgical device, a blade is rotatably connected to the inside of a U-shaped frame through a rotating rod and a bearing, one side of the rotating rod penetrates through the bearing and the U-shaped frame and extends to the outside of the U-shaped frame, a first gear is fixedly connected to the outer rod wall of the rotating rod, a fixing groove is formed in one side of an operating handle, and the fixing groove is fixedly connected to the operating handle. The inner wall of the fixing groove is fixedly connected with a first stepping motor with a self-locking structure, the output end of the first stepping motor is fixedly connected with a second gear, the second gear is meshed with the first gear, and the lower end of the side, away from the cutting edge, of the blade is fixedly connected with a telescopic arm with an adjusting buckle. The device has the beneficial effects that the second gear is driven by the first stepping motor to be meshed with the first gear, the angle of the blade is accurately regulated and controlled, and the device can be flexibly matched with different operation scenes by cooperating with the telescopic arm with the adjusting buckle; meanwhile, the elastic protection ring below the blade can effectively prevent the instrument from directly damaging normal vascular tissues.

The application provides a matrix balloon expansion system, belonging to the technical field of balloon catheters; the system comprises a catheter, a balloon assembly, a filling medium storage part and a flow control part; the balloon assembly is arranged at a position of the catheter close to a distal end at a set distance; the balloon assembly has multiple columns of balloon units arranged along the axial direction of the catheter; each balloon unit has multiple sub-balloon bodies arranged in the circumferential direction of the catheter; each sub-balloon body is used for independent inflation or deflation; the filling medium storage part is connected to the proximal end of the catheter and is used for providing filling medium into each sub-balloon body; the flow control part is connected to the filling medium storage part and is used for controlling the inflation or deflation of each sub-balloon body according to the pressure in the balloon assembly; and the system is mainly used for solving the technical problems that the shape of the whole balloon cannot be flexibly adjusted, normal blood vessels are easily damaged, and patients are obviously uncomfortable in the prior art.

An implantable medical device for implantation within a patient includes one or more sensors configured to sense an optical signal. Processing circuitry of the implantable medical device or of an external device may determine a blood pressure of the patient based on the optical signal sensed after the patient undergoes an invasive surgery, determine, based on a normal blood pressure range or a normal rate of blood pressure change over a particular period of time, that the blood pressure of the patient is abnormal, and, in response to determining that the blood pressure of the patient is abnormal, output an alert indicative of the blood pressure of the patient being abnormal.

The application provides a microfluidic device for sorting circulating tumor cells and enriching exosomes. It comprises a fixed plate, a first sorting module for separating cells and exosomes, an enrichment module for enriching exosomes, and a second sorting module for sorting normal blood cells and circulating tumor cells. The inlet of the enrichment module is connected to the exosome outlet of the first sorting module, and the inlet of the second sorting module is connected to the cell outlet of the first sorting module. The inlet of the sorting module is also provided with a sheath liquid input module for inputting sheath liquid to the second sorting module. The microfluidic device can separate cells and exosomes by using synergistic Dean flow microfluidic inertial sorting, then realize efficient enrichment of exosomes by using a pit-assisted thermal field, and separate tumor cells and normal cells by using deterministic lateral displacement technology (DLD) combined with insulating dielectrophoresis technology (i DEP).

The invention relates to the technical field of somatic variation detection, and discloses a somatic variation detection method and device based on whole exon sequencing data of normal blood, and the method comprises the following steps: obtaining variation sites of blood whole exon sequencing data from normal blood; sequentially carrying out filtering, haploid typing and haplotype classification pretreatment on the variation sites; and inputting the preprocessed variation sites into the somatic variation prediction model to obtain somatic variation in normal blood. According to the method, variation sites in whole exon sequencing data from a normal blood sample are adopted, are sequentially subjected to filtering, haploid typing and haplotype classification preprocessing and then are input into the somatic cell variation prediction model, so that the somatic cell variation detection result is obtained, and the somatic cell variation detection accuracy can be improved.

The utility model relates to a puncture-proof blood taking needle device which comprises a first puncture unit, a blood transfusion tube unit, a second puncture unit and a first protection unit. The blood sampling device has the advantages that the first protection unit is in sliding connection with the first puncture unit, the first puncture unit can be protected by pressing and sliding the first protection unit through the thumb, and the first puncture unit is exposed to be matched with the blood transfusion tube unit and the second puncture unit for blood sampling; the first protection unit is arranged at the end away from the needle head of the first puncture unit, the hand of a medical worker does not make contact with the first puncture unit, occupational exposure is reduced, the acupuncture risk is reduced, the length of the first puncture unit is not affected, and normal blood sampling can be achieved.

The invention relates to the technical field of medicines, in particular to an inhalable pharmaceutical composition containing a soluble guanylate cyclase (SGC) receptor stimulant and application of the inhalable pharmaceutical composition, and clinical adverse reactions of medicines can be remarkably reduced. According to the pharmaceutical composition, the administration dosage is reduced, on the premise that the drug effect is kept unchanged, the hypotension side effect caused in the administration process is reduced, and the normal blood pressure level is rapidly recovered after administration. The pharmaceutical composition provided by the invention needs to be combined with matched instruments for use, wherein the pharmaceutical composition comprises an SGC receptor stimulant drug and other inhalable pharmaceutic adjuvants. The lung deposition rate can reach more than 40% under the combination of a specific prescription and instruments. Through inhalation of the pharmaceutical composition, an SGC receptor stimulant can enter blood more quickly, and pharmacokinetic parameters such as Tmax are improved.

The application relates to the technical field of medical devices, and particularly provides a blood vessel covered stent, which comprises an expansion stent and an artificial blood vessel cover. The expansion stent is inserted into a branch blood vessel through a branch opening of a blood vessel. The expansion stent is provided with an entering part, a main body part and an outflow part from the branch opening to the deep part of the branch blood vessel. The artificial blood vessel cover is attached to the expansion stent. The blood vessel is expanded by the expansion stent, the blood vessel channel is prevented from being narrowed, the normal blood circulation in the blood vessel is maintained, the expansion stent and the inner wall of the blood vessel are separated by the artificial blood vessel cover, the inner wall of the blood vessel is prevented from proliferating into the inner cavity of the expansion stent, and the blood channel in the expansion stent is maintained unobstructed.

This invention belongs to the field of biomedical technology and discloses an antitumor composition and its application of an engineered bacterium expressing hemolysin and a bisphosphonate compound. The composition comprises an engineered bacterium that targets and colonizes tumors and secretes hemolysin, and a bisphosphonate compound. The engineered bacterium specifically induces tumor hemorrhage; the secreted hemolysin lyses red blood cells, which are then phagocytosed by macrophages. The hemolysin then self-assembles with the bisphosphonate compound intracellularly, generating a large amount of reactive oxygen species, thereby remodeling the antitumor phenotype of tumor-associated macrophages and achieving an antitumor effect. Furthermore, the engineered bacterium does not induce bleeding in normal blood vessels and therefore has no effect on normal cells.

The application discloses a method for obtaining morphological parameters of saccular aneurysms based on a tumor neck curved surface, a computer device and a readable storage medium. The method comprises the following steps: obtaining a three-dimensional saccular aneurysm blood vessel model; constructing a corresponding normal blood vessel according to the three-dimensional saccular aneurysm blood vessel model; using a magnification coefficient to isometrically enlarge the normal blood vessel to obtain an enlarged normal blood vessel; performing a subtraction operation on the three-dimensional saccular aneurysm blood vessel model and the enlarged normal blood vessel to obtain a tumor neck curved surface; segmenting to obtain a saccular aneurysm model; and further obtaining morphological parameters of the saccular aneurysm, wherein the morphological parameters comprise a tumorization rate; and the magnification coefficient is obtained in the following manner: continuously adjusting the magnification coefficient until a minimum change value of the tumorization rate is obtained. The tumor neck curved surface obtained by the application is more accurate and more in line with the actual situation than a tumor neck plane, has better applicability, and the morphological parameters of the saccular aneurysm model obtained by the application are more accurate.

Provided is a method for estimating a factor of coagulation abnormality in a blood sample. The method comprises the following steps: 1) calculating a parameter Ps related to a coagulation reaction of a specimen S and a parameter Pm related to a coagulation reaction of a specimen M; 2) inferring, on the basis of Ps, whether the specimen S is positive for a coagulation factor inhibitor; and 3) inferring, on the basis of Pm, or Ps and Pm, that a lupus anticoagulant is positive or a coagulation factor is deficient, from the specimen (S) for which the coagulation factor inhibitor is not inferred to be positive in step 2). The specimen S is a blood specimen to be tested having a prolonged coagulation time, the specimen M is a mixed specimen of the specimen S and the specimen N, and the specimen N is a normal blood specimen. Ps is calculated on the basis of the measurement point or time at which the coagulation reaction curve or the coagulation velocity curve of the specimen S reaches a prescribed value, and Pm is calculated on the basis of the measurement point or time at which the coagulation reaction curve or the coagulation velocity curve of the specimen M reaches a prescribed value.

The utility model discloses a prone position ventilation pillow capable of preventing pressure sores, and relates to the technical field of medical equipment. The adjusting mechanism comprises a clamping and locking assembly, a supporting assembly and a soft cushion assembly, the clamping and locking assembly comprises a limiting groove formed in the fixing plate, the limiting groove is in sliding connection with a limiting block, the limiting block is fixedly connected with a spring, the spring is fixedly connected with a bearing block, and the bearing block is fixedly connected with the supporting assembly. The bearing block is fixedly connected with a clamping block. Through the adjusting mechanism, a person can turn over the turning plate by pressing the clamping blocks to drive the ventilation pillow to incline to a proper angle and clamp the two clamping blocks into the specific clamping holes to complete limiting by utilizing the elastic acting force of the spring, so that a patient can conveniently lie on the ventilation pillow in a prone position, and the legs, the head and the abdomen are mutually inclined; the two arms can also annularly hang down the two sides of the ventilation pillow and the turnover plate, normal blood circulation of the whole body of the patient is ensured, and the practicability of the device is improved.

The present application relates to the technical field of medical equipment, and discloses a gradient expansion coronary expansion balloon and a use method, which comprises a main balloon body, the side surface of the main balloon body is respectively provided with a front end auxiliary balloon body and a rear end auxiliary balloon body along the axial direction thereof, the front end auxiliary balloon body is used for forming support for the normal blood vessel at the distal end of the lesion segment, and the rear end auxiliary balloon body is used for forming support for the normal blood vessel at the proximal end of the lesion segment, so as to realize gradient support in cooperation with the main balloon body, and a multi-cavity delivery catheter extending along the axial direction of the main balloon body is arranged on the main balloon body. The present application can form gradient support for the blood vessel at the lesion segment and the proximal end and the distal end of the lesion segment, avoids the rapid elastic retraction of the blood vessel wall to the lesion segment after the balloon is deflated, increases the effective surface area of the drug carrying, realizes the precise local delivery of high-dose drugs, and can independently adjust the inflation pressure of the main balloon body, the front end auxiliary balloon body and the rear end auxiliary balloon body.

This utility model relates to the field of respiratory masks, specifically a simple oxygen nebulizer mask for bronchoscopy. It includes a mask body connected to an oxygen tubing, a breathing port and a bronchoscope inlet, a connecting strap connecting the mask body to a breathing cap, an airtight plug connected to the breathing cap, the airtight plug being detachably connected to the breathing port, a connecting strip connecting the breathing cap to an inlet cap, a sealing plug connected to the inlet cap, and a detachably connected sealing plug to the bronchoscope inlet. The mask body has a sliding groove, a slider slidably connected to the side wall of the groove, a spring connected to the slider, and a sealing strip connected to the slider, which is slidably connected to the mask body. This utility model can prevent oxygen leakage from the bronchoscope inlet, ensuring the patient can continuously inhale a sufficient concentration of oxygen, maintain normal blood oxygen saturation, reduce risks during the examination, and provide basic support for the smooth conduct of the examination.

The invention discloses a stent device with a folded skirt and a heart valve, the stent device comprises a stent and is further provided with a flexible skirt, and the skirt has a spreading state in which the skirt axially extends and surrounds the periphery of the stent before release; and in the stacking state, the annular peripheral leakage plugging part is driven by deformation when the stent is released and is folded and stacked in the axial direction of the released stent to form the annular peripheral leakage plugging part. By means of the peripheral leakage prevention technology, the interventional stent is more matched with the inner wall of a blood vessel, the stent is not prone to displacement and is more stable, the range of applicable people is widened, additional risks of operations are reduced, and complications such as peripheral leakage and thrombus are prevented and treated. Better hemodynamic performance is provided, the host endothelial cell climbing and covering function is enhanced, the occurrence probability of endocarditis is reduced, and the normal blood supply function of heart and blood vessels is recovered.

The application provides a portable non-closed oxygen inhalation mask, and relates to the field of medical auxiliary devices; the oxygen inhalation mask comprises: a flexible mask used for being worn on the head and face of a user, which is provided with a connecting port at a position opposite to the mouth and nose of the user; a supporting framework is arranged in the flexible mask; a cup cover is arranged on the side of the flexible mask away from the face of the user, and the open end of the cup cover is in sealing connection with the connecting port; an air inlet for being connected with an oxygen supply pipe is arranged on the side wall or the bottom of the cup cover; wherein the volume of the cup cover can be adjusted, and the overall weight is relatively light to avoid sliding. The application can improve the oxygen concentration around the mouth and nose of the user to improve the oxygen utilization rate; reduce the damage of the oxygen flow to the nasal mucosa and the face, thereby improving the oxygen inhalation comfort; improve the carbon dioxide concentration around the mouth and nose, maintain the relatively normal blood carbon dioxide partial pressure through carbon dioxide reabsorption to promote the red blood cells to release oxygen to tissue cells, and improve the internal respiration efficiency of each organ of the body and the effect of oxygen inhalation treatment.

This invention belongs to the fields of biotechnology and precision oncology, and discloses a serum-free amplification method for circulating tumor cells (CTCs) based on apoptosis selectivity, along with a dedicated culture medium. This addresses the problems of existing CTC technologies, which rely on physical capture leading to impaired cell viability and low culture success rates. The invention involves mild density gradient centrifugation and erythrocyte lysis of anticoagulated whole blood from patients to obtain a mixed population of leukocytes containing CTCs. This population is then seeded into a serum-free, selective apoptosis medium for three-dimensional culture. The medium contains apoptosis inhibitors and specific growth factors. Utilizing the difference between tumor cells' anti-apoptosis and normal blood cells' susceptibility to apoptosis, leukocyte apoptosis is induced within 4-7 days, while simultaneously supporting CTC survival, adhesion, and clonal proliferation, enabling the amplification of individual CTCs into cell clusters. This method preserves the original activity and heterogeneity of CTCs, is simple to operate, and has good reproducibility, providing a highly active cell source for CTC molecular identification, drug sensitivity testing, and metastasis mechanism research.