6939 results about "Polysaccharide" patented technology

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention discloses a probiotic-polyphenol nanoparticle colon-targeted co-delivery system as well as a preparation method and application thereof, and belongs to the field of biological medicines. The FCPs and hyaluronic acid (HA) are combined through non-covalent interaction, so that the hydrogel has colon targeting property and mucosal adhesion at the same time, and delivery of probiotics is facilitated. The hydrogel (HF) based on polysaccharide has good biocompatibility, and can be used for effectively encapsulating the probiotics and the natural products. Then, the PDA-TH NPs and BA are incorporated into the polysaccharide chain network of HF, and finally the BA-HF-PDAT targeted co-delivery system is constructed. The BA (at) HF-PDAT targeted co-delivery system can protect BA from serious gastrointestinal stress, and is jointly assembled with PDT-TH NPs to realize dual slow release of thymol (Thy), so that the treatment effect of BA and Thy is improved, and the BA (at) HF-PDAT targeted co-delivery system contributes to treatment of clostridium difficile infection (CDI).

The invention belongs to the technical field of catalysts, and particularly relates to an alumina-based catalyst for anthraquinone hydrogenation and a preparation method thereof.The preparation method comprises the following steps that 1, aluminum salt and PVP are added into deionized water, and an aluminum salt mixed solution is obtained; (2) adding ammonium salt and cyclic polysaccharide micromolecules into deionized water to obtain an ammonium salt mixed solution; (3) dropwise adding the aluminum salt mixed solution into the ammonium salt mixed solution while stirring, and performing aging, suction filtration, drying and calcination to obtain an alumina carrier; (4) impregnating the alumina carrier with a palladium salt solution; and (5) drying, roasting and reducing the impregnated alumina carrier to obtain the alumina-based catalyst for anthraquinone hydrogenation. Macromolecular PVP (Polyvinyl Pyrrolidone) and cyclic polysaccharide micromolecules are used as composite template agents for ammonium-aluminum coprecipitation, so that not only is an alumina carrier material with a high specific surface area prepared, but also the loading capacity on palladium is improved, the dispersion of palladium is promoted, and the hydrogenation efficiency is favorably improved.

The invention relates to double-layer integrated asymmetric viscous hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The invention relates to a preparation method of double-layer integrated asymmetric viscous hydrogel, which comprises the following steps: adding natural macromolecular polysaccharide and a polymeric monomer into a salt solution, stirring at room temperature, then adding an initiator and a cross-linking agent, stirring and polymerizing to obtain the double-layer integrated asymmetric viscous hydrogel. Through a one-pot method, a salt component is introduced into a hydrogel system, and the hydrogel has a double-layer structure with asymmetric viscosity under the salting-out action; in addition, the hydrogel is doped with a carbon nanotube with the surface modified by polydopamine and silver nanoparticles, so that the mechanical property and the conductivity of the hydrogel are remarkably improved. The preparation method provided by the invention is simple, is suitable for various hydrogel systems, has good universality and is easy to popularize and apply.

The invention relates to a poly-(2-oxazoline) epoxy derivative and application thereof, and belongs to the technical field of crosslinking. The invention provides a poly-(2-oxazoline) epoxy derivative, which is completely different from existing cross-linking agents such as 1, 4-butanediol diglycidyl ether (BDDE), divinyl sulfone, glycidyl methacrylate and the like in structure, and has the advantages that the structure is simple; the polymer can be used as a cross-linking agent for cross-linking natural high-molecular polymers such as polysaccharide (such as hyaluronic acid) and protein and synthetic high-molecular polymers such as polyethylene glycol and polyvinyl alcohol. Compared with an existing cross-linking agent, the poly-(2-oxazoline) epoxy derivative has the advantages that the poly-(2-oxazoline) epoxy derivative can be easily subjected to a cross-linking reaction with high-molecular polymers such as hyaluronic acid, the prepared cross-linked substances such as sodium hyaluronate gel are more stable and uniform, the performance of the prepared cross-linked substances is easier to regulate and control and the like, and the poly-(2-oxazoline) epoxy derivative has a great application prospect.

The invention relates to the technical field of Brassica rapa polysaccharide detection, and discloses a quantitative detection method of Brassica rapa polysaccharide based on near infrared spectroscopy. The method comprises the following steps: acquiring near infrared spectrum data of a brassica rapa sample, and dividing detection stages by combining spectral characteristics to obtain a plurality of detection stage identifiers; performing differential mapping on the spectrum load spectrum according to the identifier to obtain an optical parameter set of each stage; constructing a staged near infrared spectrum quantitative model by using the set; performing importance weighting on the original spectral data according to the identifier to generate optimized spectral data; and extracting polysaccharide related characteristics from the optimized spectral data through a staged model, and outputting a predicted value of the content of the Brassica rapa polysaccharide. According to the method, through stage division, differential mapping and data weighting, the spectral data utilization rate and the model suitability are improved, a targeted technical path is provided for quantitative detection of Brassica rapa polysaccharide, and the method can be used for quality control of Brassica rapa related products.

The invention relates to the technical field of food processing, and discloses casein-tea polysaccharide-polyphenol composite nanoparticles and a preparation method of the casein-tea polysaccharide-polyphenol composite nanoparticles, and the preparation method comprises the following steps: mixing large yellow tea polysaccharide with a casein solution to obtain a CS / TPS mixed solution; performing glycosylation modification on casein in the CS / TPS mixed solution by adopting a Maillard reaction to prepare a casein-polysaccharide copolymer solution; the preparation method comprises the following steps: mixing a plurality of catechins, dissolving the catechins in ultrapure water to obtain a mixture stock solution, mixing the mixture stock solution and a casein-polysaccharide copolymer solution according to a volume ratio of 1: 16, stirring for 1 hour for self-assembly, pre-freezing the stirred solution at-80 DEG C, and freeze-drying for 48 hours to obtain CS-TPS-MIX nano-composite powder. According to the invention, a polyphenol delivery system with a dual antioxidant synergistic mechanism is constructed through a Maillard reaction mediated nano-composite technology, and the system is stable in structure, so that the release of polyphenol is more controllable.

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention relates to an application of sea cucumber intestine polysaccharide in preparation of uric acid lowering drugs. The preparation method of the sea cucumber intestine polysaccharide comprises the following steps: performing enzymolysis treatment on sea cucumber intestines by using papain, performing enzyme deactivation, performing centrifugation, and taking supernate; and carrying out graded alcohol precipitation on the supernate, centrifuging, taking precipitate, deproteinizing and freeze-drying. The preparation method is simple, green and environment-friendly. The sea cucumber intestine polysaccharide prepared by the method can effectively inhibit the activity of xanthine oxidase; the sea cucumber intestine polysaccharide has no toxic effect on HK-2 cells. The compound has a uric acid reducing effect on HK-2 hyperuricemia cells.

The invention belongs to the technical field of food processing, and particularly relates to a curcumin-loaded protein-polysaccharide nanoparticle delivery system, a preparation method thereof and application of the curcumin-loaded protein-polysaccharide nanoparticle delivery system in neuroprotection. The preparation method comprises the following steps: mixing and dissolving sodium caseinate with one of glucan and pectin, hydrating at 0-4 DEG C for 12-24 hours, and performing ultrasonic and microwave treatment to obtain a pretreatment solution; adjusting the pH value, and heating the pretreatment solution to obtain covalent grafted particles; the protein-polysaccharide covalent grafted particles obtained in the second step are encapsulated with curcumin through a pH shift method, and the protein-polysaccharide-curcumin nanoparticles are obtained. The invention provides a method for carrying out Maillard reaction on sodium caseinate-polysaccharide under the assistance of ultrasound and microwaves, reaction parameters are optimized, and a preparation method of an efficient and stable curcumin delivery system and application of the curcumin delivery system in neuroprotection are constructed.

The invention relates to the technical field of gel materials, and discloses whitening gel based on biological polysaccharide and a preparation method thereof, the whitening gel comprises the following raw materials: hyaluronic acid, deionized water and thunberg fritillary bulb polysaccharide microcapsules; wherein the bulbus fritillariae thunbergii polysaccharide microcapsule is prepared by taking konjac glucomannan, chitosan and perillaldehyde modified hyaluronic acid as a shell layer and embedding bulbus fritillariae thunbergii polysaccharide and glabridin as a core material; the perillaldehyde modified hyaluronic acid is prepared by reacting alkenyl hyaluronic acid with perillaldehyde under the action of an initiator; the alkenyl hyaluronic acid is prepared by reacting hyaluronic acid with glycidyl methacrylate under the action of a catalyst. The prepared whitening gel has excellent whitening, moisturizing, anti-oxidation and anti-aging effects, effective components are not prone to loss, and the whitening gel can be applied to the skin surface for a long time, plays a whitening role and delays the aging process of the skin.

The invention discloses a polygonatum sibiricum polysaccharide extracting solution concentration scraper, and belongs to the technical field of concentration scrapers. Comprising a hollow rod, a mounting box is fixedly connected to the top end of the hollow rod, a connecting plate is fixedly connected to the upper surface of the mounting box through a plurality of bolts, and a hollow main shaft is fixedly connected to the center of the upper surface of the connecting plate; air is supplied to the fixing box through the air conveying pipe, the U-shaped pipe, the communicating pipe and the hose, the piston plate is pushed to drive the supporting rod and the scraper body to extend outwards, initial close contact between the scraper and the inner wall of the evaporation barrel is achieved, and meanwhile a second spring in the supporting rod can adjust contact pressure in a self-adaptive mode to ensure that the supporting rod is tightly attached to the inner wall of the evaporation barrel; meanwhile, the first spring is matched to drive the scraper body to be stably retracted when gas is stopped, the problem of gaps after long-term use is thoroughly solved, uneven film formation caused by missing scraping is avoided, materials are prevented from being retained in the gaps and being overheated and carbonized, and the purity and activity of the polygonatum sibiricum polysaccharide are guaranteed.

The invention discloses a compound feed for raising plateau live pigs and a preparation method thereof, and relates to the technical field of feed processing. The compound feed for raising the plateau live pigs is prepared from manganese-selenium compound modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles, aspergillus oryzae, soybean meal powder, corn flour, wheat bran, mountain flour, calcium hydrophosphate, table salt and multivitamins. Manganese-selenium composite modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles and aspergillus oryzae are subjected to co-fermentation culture to form a uniform, stable, loose and porous three-dimensional network fermentation substrate composite system, so that the damage of gastric acid to components is reduced, the targeted release of intestinal tracts is realized, and the bioavailability is improved. The composite functional additive which is high in nutrition, easy to absorb, anti-oxidation, anti-anemia, anti-ultraviolet radiation and the like is obtained, and the adverse effects of low temperature, oxygen deficit and ultraviolet factors on animal growth and feed oxidative rancidity are effectively reduced.

The present invention relates to the field of pharmaceutical technology, and more particularly to the use of Morinda officinalis polysaccharide in preparing a product for improving renal fibrosis. The preparation method of the Morinda officinalis polysaccharide comprises the following steps: (1) extracting Morinda officinalis from water, filtering, and obtaining a supernatant; (2) subjecting the supernatant to alcohol precipitation to obtain a precipitate; and (3) dialyzing and removing protein from the precipitate. The Morinda officinalis polysaccharide has a significant therapeutic effect in improving renal fibrosis.

The invention belongs to the technical field of adhesives, and particularly relates to a preparation method of a polysaccharide-based reversible bonding adhesive for constructing a dynamic covalent bond network. The preparation method comprises the following steps: by taking lipoic acid and sodium alginate as reactants and ferric chloride as a cross-linking agent, carrying out cross-linking reaction under a stirring condition to obtain a lipoic acid adhesive, namely the polysaccharide-based reversible bonding adhesive. According to the invention, renewable biomass resources such as sodium alginate, hyaluronic acid, chitosan and the like are used as main raw materials, so that the adhesive is expected to replace a traditional synthetic adhesive depending on a petroleum base, accords with a sustainable development strategy, contributes to reducing consumption of fossil resources, avoids release of toxic substances such as formaldehyde and the like in the synthesis and use processes, and has a good application prospect. And the method is more environment-friendly. The bio-based polysaccharide raw material has good biocompatibility and degradability, and an ideal base material is provided for developing a green and safe biomedical adhesive.

The invention discloses a high-valued graded utilization method of marine shells, and belongs to the technical field of comprehensive utilization of biological resources and preparation of functional materials. The method comprises the following steps: cleaning, air-drying and superfine grinding marine shell wastes such as abalone shells, and extracting water-soluble organic components in the marine shell wastes by adopting mild water extraction, so that the obtained extract is rich in protein, polysaccharide or small molecule peptide, has good biological activity, and can be used for preparing functional materials such as biomimetic mineralization, tissue repair and the like; extracted shell powder residues are subjected to heat treatment or chemical activation treatment and then compounded with natural polymer materials such as chitosan and sodium alginate, and the composite adsorbent which is loose in structure and large in specific surface area is prepared and can be used for removing dye, heavy metal, antibiotics and other water pollutants. According to the invention, separate utilization of organic components and inorganic framework materials in the marine shells is realized, and the method has the characteristics of environmental protection, efficient resource conversion and multifunctional application, and has a good industrial popularization prospect.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The invention belongs to the field of detection and analysis, and relates to a method for separating and detecting enantiomers in (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride. According to the method, HPLC is adopted for separation and detection, a polysaccharide derivative chiral chromatographic column is selected as a chromatographic column, a mobile phase A is a weak base aqueous solution, and a mobile phase B is acetonitrile and / or methanol. The method is strong in specificity, high in separation degree, high in accuracy, stable, reliable and high in sensitivity, and a simple, accurate, rapid and reliable detection method is provided for control of impurities in a starting material of (S)-2-amino-3-[2-(tert-butoxy)-2-oxoacetamido] tert-butyl propionate hydrochloride.

The invention relates to a special fertilizer suitable for saline-alkali soil and a preparation method thereof. The fertilizer is granular and has a multi-layer structure which is sequentially arranged from inside to outside: a nutrition core layer, a controlled release film layer, a soil improvement fast exchange layer and a microorganism attaching layer. The nutrition core layer contains nitrogen, phosphorus, potassium and medium trace elements, and meets the basic nutrient requirements of crops; the controlled release film layer is self-assembled layer by layer by adopting alginate / chitosan, a humic acid-ferrous complex and a breakable cross-linking point are inserted into the film, and starch is sprayed on the outer layer, so that an ionic strength / alkalinity dual-response controlled release system is formed; the soil improvement quick exchange layer contains microcrystalline gypsum and ferrous sulfate; the microbial epiphytic layer fixes salt-tolerant phosphate solubilizing bacteria, potassium bacteria and exopolysaccharide-producing bacillus by using a charcoal-zeolite carrier, and the bacteria are distributed on the surfaces of the particles in a dotted manner. The fertilizer realizes organic combination of soil improvement, fertilizer supply and microbial regulation and control, can significantly reduce the soil salinity and alkalinity in a saline-alkali soil environment, and improves the fertilizer utilization rate and the crop growth performance.

The invention relates to the technical field of biology, in particular to a polyvalent pneumococcal polysaccharide-protein conjugate composition for preventing pneumococcal infection and VZV infection as well as a preparation method and application of the polyvalent pneumococcal polysaccharide-protein conjugate composition. According to the composition containing the multivalent pneumococcal polysaccharide-protein conjugate, an adopted carrier protein is a VZV recombinant protein with immunogenicity; the pneumococcal polysaccharide is connected with the VZV recombinant protein through a covalent bond; the VZV recombinant protein is selected from gE protein, and the amino acid sequence of the VZV recombinant protein is shown as SEQ ID NO: 3 or SEQ ID NO: 4. Aiming at the main epidemic condition of specific serotype pneumococcus in China, serotype combinations of 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F are creatively selected, so that the composition containing the multivalent pneumococcus polysaccharide-protein conjugate, which is designed aiming at the epidemic condition of the specific serotype pneumococcus in China, is developed.

The invention discloses an oral care composition containing ampelopsis japonica polysaccharide and application of the oral care composition in inhibiting gingival bleeding. The oral care composition comprises ampelopsis japonica polysaccharide and an orally acceptable carrier, wherein the pH (Potential of Hydrogen) range of the oral care composition is 5.9 to 7.7. The invention also discloses an application of the ampelopsis japonica polysaccharide in the oral care composition for inhibiting gingival bleeding. The invention discloses application of silicon dioxide with a high oil absorption value in an oral care composition for improving the gingival bleeding inhibiting effect of ampelopsis japonica polysaccharide. The combination of the high-oil-absorption-value silicon dioxide and the low-oil-absorption-value silicon dioxide is applied to the oral care composition for improving the gingival bleeding inhibiting effect of the ampelopsis japonica polysaccharide; the invention further discloses application of sorbitol in the oral care composition for improving the effect of inhibiting gingival bleeding by the ampelopsis japonica polysaccharide. The oral care composition has a hemostatic effect and can effectively solve the problem of gingival bleeding.

The invention discloses organic nano composite hydrogel as well as a preparation method and application of the organic nano composite hydrogel, and belongs to the technical field of biological medicine delivery. The polysaccharide-based nano prodrug in the composite hydrogel can be subjected to surface protonation under the stimulation of a tumor extracellular slightly acidic environment, so that cellular uptake is promoted, low-pH / high-concentration glutathione in tumor cells is responded, intracellular aggregation and controllable release of the nano drug are realized, the retention time of the drug in the cells is prolonged, and the bioavailability of the drug is improved. The technical effect of killing tumor cells through combined chemotherapy is achieved. According to the preparation method disclosed by the invention, the release of the polysaccharide-based nano prodrug from the hydrogel and the tissue infiltration capacity are accelerated by adopting fluorinated orthoester, the infiltration of the polysaccharide-based nano prodrug to cells is increased, and then the drug-loaded compound is encapsulated by using gellan gum, so that the slow-release effect of the polysaccharide-based nano prodrug is enhanced; therefore, the efficient enrichment of the medicine at the tumor part is realized.

The present invention relates to a biological polysaccharide hydrogel, a preparation method therefor and an application thereof. The biological polysaccharide hydrogel of the present invention is formed by means of the mixing and reaction of two solutions; the first solution is a polysaccharide derivative solution having an aldehyde on a side chain; the second solution is a polysaccharide derivative solution having a primary amine on a main chain or a side chain, or a small molecule compound solution having two or more primary amines. The preparation method of the present invention comprises preparing the first solution and the second solution, and then mixing the first solution and the second solution to form a transparent biological polysaccharide hydrogel. The biological polysaccharide hydrogel of the present invention can be applied as a vitreous substitute.

The invention provides a method for efficiently extracting tea polyphenol and tea polysaccharide and application, and belongs to the technical field of plant extraction.The extraction method comprises the following steps that tea leaves are pre-cooled and rolled and cut to obtain pretreated tea leaves, then the pretreated tea leaves are added into water to be mixed, biological enzyme is added for ultrasonic enzymolysis, filtering is conducted, and filtrate and tea residues are obtained; adding tea residues into water, mixing, adding an auxiliary agent, and carrying out low-temperature plasma treatment and filtration to obtain a filtrate; combining the filtrates, concentrating, precipitating, centrifuging, and collecting precipitate and supernate; washing and drying the precipitate to obtain a tea polysaccharide extract; and concentrating, extracting, adsorbing, eluting, re-concentrating and drying the supernate to obtain the tea polyphenol extract. The extraction method disclosed by the invention can be used for efficiently extracting the tea polyphenol and the tea polysaccharide in the tea leaves, the product yield and purity are high, and the tea polyphenol and the tea polysaccharide can be used for preparing health-care products for relieving diabetes and polyuria.

The invention discloses an application of a polysaccharide monomer separated from schisandra chinensis in preparation of a PD-L1 + TAMs activator. A large number of experiments show that the polysaccharide monomer schisanan B separated from schisandra chinensis can effectively activate PD-L1 + TAMs cell subsets, so that the phagocytosis of TAMs is enhanced, and the proliferation of tumor cells is inhibited; therefore, it is determined that the polysaccharide monomer schisanan B separated from schisandra chinensis can serve as a natural PD-L1 + TAMs cell subset activator to be used for treating cancers such as non-small cell lung cancer, intestinal cancer, melanoma, urothelial carcinoma or liver cancer, and the polysaccharide monomer schisanan B has the advantages of being free of toxic and side effects, low in price, wide in adaptive patient population and the like.

The invention belongs to the field of medical instrument coloring agents, and provides a natural anthocyanin endoscope coloring agent. According to the invention, the innovative design of combining a ternary color-stabilizing compound formed by anthocyanin, a co-chromophore and hydroxypropyl beta cyclodextrin with a chitosan sodium alginate polysaccharide system and a composite buffer system is adopted, and the viscosity is controlled to be 200-320 millisecond, the particle size of spray droplets is 40-60 microns, and the thickness of a thin film is 10-30 microns through specific molar ratio compatibility and a genipin crosslinking technology. Through accurate regulation and control, the excellent performance that a double-network thin layer is formed under the calcium ion environment that the absorbance retention rate at 520 nanometers is not lower than 90% and the elution rate is not lower than 90% under the irradiation of hundred thousand lux white light is achieved, the technical problems that a traditional endoscope coloring agent is poor in safety, low in stability and insufficient in mucous membrane contrast ratio are solved, and the wide clinical application value is achieved.

The invention belongs to the technical field of bioengineering technologies, and particularly relates to heparanase sulfate as well as a preparation method and application thereof. The amino acid sequence of the heparanase sulfate (HPSE) is as shown in SEQ ID NO. 2. The HS carbohydrate chain substrate capable of being specifically degraded by the heparan sulfate enzyme (WJS HPSE) provided by the invention is white jade snail polysaccharide (WJS PS) containing an IdoA2S-GlcNAc disaccharide repeating unit, heparin or HS. In addition, the heparan sulfate enzyme (WJS HPSE) provided by the invention is high in thermal stability and has optimal enzyme activity at the temperature of 57-67 DEG C and the pH value of 4.0, and the stability and reutilization efficiency of the heparan sulfate enzyme in a catalytic process are remarkably improved, so that the application value of the HPSE is improved.

The invention discloses a saline-alkali soil improvement and disease resistance double-effect controlled release fertilizer and a preparation method thereof. The fertilizer comprises 18-22% of biochar, 12-18% of furfural residues, 8-12% of cow dung compost and 6-10% of potassium humate. 5-7% of aluminum sulfate, 4-6% of sulfur and 3-5% of zeolite; the microbial inoculum comprises the following components in percentage by weight: 1.5 to 2.5 percent of saline-alkaline bacillus, 0.8 to 1.2 percent of bacillus subtilis and 0.8 to 1.2 percent 8-12% of a sodium polyacrylate coating, 4-6% of a sulfur coating and 1-3% of polyglutamic acid; 0.8 to 1.2 percent of humic acid chelated iron, 0.8 to 1.2 percent of algal polysaccharide and 0.8 to 1.2 percent of urease inhibitor; 0.8 to 1.2 percent of zinc sulfate, 0.5 to 1.0 percent of manganese sulfate and 0.5 to 1.0 percent of borax. The fertilizer can reduce the pH and conductivity of soil, increase the organic matter content of the soil and effectively improve the physicochemical properties of the soil, meanwhile, by adding the microbial agent, the disease resistance of crops can be effectively improved, the morbidity and disease index of the crops can be reduced, and nutrient release of the fertilizer has a sustained and controlled release characteristic; the initial release rate and the cumulative release rate of nitrogen are both lower than those of common chemical fertilizers, and the cumulative release rate of nitrogen is moderate within 60 days, so that the nutrient requirements of crops in different growth stages can be met.

The invention relates to the technical field of functional cosmetic raw materials, in particular to a cactus polysaccharide composition as well as a preparation method and application thereof. According to the cactus polysaccharide composition disclosed by the invention, the cactus polysaccharides with specific number-average molecular weight are combined according to a specific proportion, so that the cactus polysaccharide composition has a more comprehensive anti-irritation effect and has the characteristics of high stability and better skin feeling and solubility. The cactus polysaccharide can be prepared through a simple process, exogenous components do not need to be introduced, after the cactus aqueous extract is subjected to enzymolysis through amylase, the cactus polysaccharide with the specific number-average molecular weight is screened and combined according to the specific proportion, the anti-irritation effect requirement can be met, and safety is better. The cactus polysaccharide composition can be applied to cosmetics / pharmaceuticals as an anti-irritation raw material.