The present invention discloses a
highly sensitive cTnI detection kit and application based on the ROP
signal amplification strategy triggered by FFAPO, and the kit includes an
electrode, MPA, EDC, NHS, Ab1, BSA, Ab2, GA,
potassium methoxide, FFAPO, and LiClO4. The present invention adopts a sandwich structure to introduce cTnI, and connects the initiator
glycolic acid of the ROP reaction to Ab2 through an
esterification reaction, so that a large amount of
ferrocene electroactive
polymer is grafted to the
electrode surface through the ROP reaction, and successfully establishes a kit for detecting cTnI with high sensitivity and strong specificity. Experiments have shown that the
linear range of the detection of the present invention is 1pg / mL to 1μg / mL, the
correlation coefficient is 0.997, the
detection limit is 57.14fg / mL, the stability reaches 94.8% of the initial value after continuous storage for two weeks, and the anti-interference reaches 96.2% to 103.1%. At the same time, it has good selectivity and reproducibility, which lays a foundation for the later application of cTnI detection in the medical field and early diagnosis of acute
myocardial infarction.