649 results about "Biomaterial" patented technology

A system for preparation of an implant and ab interno insertion of the implant into an eye including a handle having one or more actuators and an elongated shaft having an outer sheath and an elongate member positioned within a lumen of the tubular outer sheath. The system includes a recess sized for holding a patch of material fixed relative to the handle and a cutting member movable relative to the handle and to the recess. The cutting member cuts the patch of material into an implant as the cutting member moves towards a cutting configuration. The implant, once cut, is axially aligned with the lumen of the tubular outer sheath. The inner elongate member is movable relative to the tubular outer sheath to advance the implant into a deployment position in the lumen of the tubular outer sheath for delivery into the eye. Related devices and methods are provided.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

An acellular product may be derived from human placenta and may be used in various scenarios for wound healing. Because the product may be acellular, the product may be processed for storage and transportation with minimal degradation. The product may include various scaffolding such as biomaterials or human tissue, and the scaffolding may be infused with various plasmas and agents. The cell-free treatment may maintain the biological activity of many therapeutic agents found within cells and may possess multiple structural components to support cellular attachment. The structural components or scaffolds may function as a reservoir of highly diffusible chemotactic and cellular-programming factors that may be useful to treat injury and disease.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention belongs to the technical field of medical biological materials, and particularly relates to a sodium hyaluronate gel regeneration filler for injection and a preparation method and application thereof.The sodium hyaluronate gel serves as a carrier to disperse acellular matrix particles, the sodium hyaluronate gel forms a micron-sized porous structure in the cross-linking process through a microbubbling technology, and the sodium hyaluronate gel is prepared into the sodium hyaluronate gel regeneration filler for injection and the preparation method and application of the sodium hyaluronate gel regeneration filler. A local cross-linked network is strengthened by combining a secondary cross-linking process, high elastic modulus is realized while the content of sodium hyaluronate is reduced, so that the porous structure has excellent instant filling and shaping capability and controllable degradation behavior, the porous structure can promote permeation of hyaluronidase, the degradation period of the hyaluronidase is matched with the tissue regeneration process, and the tissue regeneration effect is improved. And the acellular matrix particles can continuously induce adhesion, migration, proliferation and differentiation of endogenous cells of tissues, synergistically promote in-situ tissue regeneration, effectively reduce repeated injection requirements and reduce long-term complication risks, and have a good application prospect in soft tissue filling and repairing.

The present disclosure relates generally to methods of inhibiting a tripartite VAP-A, ORP3 and Rab7 (VOR) protein complex in multicellular organisms, to methods of identifying agents which inhibit such complex and to the medical use of those agents. Inhibition of the VOR complex causes interference with at least one mechanism of intercellular communication, wherein the intercellular communication is mediated by receptor-ligand interaction and / or EVs, and viral infection involving the transport of endocytosed biomaterials to the nucleus of recipient cells.

An endoscopic printing device comprising: a printing head having: a nozzle for outputting a biomaterial; a central conduit connectable to a source of the biomaterial and connected to the nozzle, the central conduit being flexible; ribs disposed around the central conduit and spaced apart from one, a rib of the ribs defining three or more guiding apertures extending axially through the rib, the ribs being interconnected to one another; and cables extending through the guiding apertures, ends of the cables attached to the central conduit, the cables configured to be engaged to an actuator for controlling motion of the endoscopic printing head; and an actuation system engaged to the cables, the actuation system including actuators each engaged to a respective one of the cables, the actuators operable to vary a tension in one or more of the cables relative to a remainder of the cable to bend the central conduit.

The present application relates to a kind of composite rotator cuff patch and its preparation method and application, belong to the field of biomaterials technology.The structure layer and biological layer are all biological medical materials, do not need to introduce crosslinking agent, and by the structure layer and biological layer alternate composite design, avoid the problem that traditional synthetic material is not degradable or short degradation period, with controllable degradation period, improve the tear resistance and mechanical support of patch, effectively reduce the risk of inflammatory response, improve the biocompatibility and safety of patch, finally realize the applicability and effect of improving composite rotator cuff patch in clinical application.

Methods and devices for capturing and analyzing aerosolized particles in exhaled breath characteristic of a respiratory disease to enable rapid, low-cost point of care assays for several diseases including respiratory tract diseases such as COVID-19 are disclosed. The disclosed methods and systems selectively capture aerosolized particles using a packed bed column. The captured particles are then eluted using solvents and analyzed using analytical devices including MALDI-TOFMS.

The invention discloses a functional osteochondral repair material which comprises a cartilage layer, a transition layer and a bone layer which are sequentially connected, the cartilage layer takes hyaluronic acid, I-type collagen and chitosan as base materials, the transition layer takes hydroxyapatite and a polylactic acid-glycolic acid copolymer as base materials, and the bone layer takes hyaluronic acid, I-type collagen and chitosan as base materials. The bone layer takes beta-tricalcium phosphate, hydroxyapatite and a polylactic acid-glycolic acid copolymer as base materials, the invention relates to the technical field of medical biological materials, by designing the organic-inorganic composite transition layer, the gradient connection cartilage layer and the bone layer, and the interfacial shear strength is greater than or equal to 0.5 MPa, the problem of interlayer stripping of a traditional layered material is effectively solved, and the medical material has the advantages of high mechanical strength, high mechanical strength and high mechanical strength. According to the selective laser sintering 3D printing material, the shearing stress and the compression stress during joint movement can be borne, mature medical material production equipment such as freeze drying, compression molding and sintering is adopted in the preparation process, large-scale batch production can be achieved, meanwhile, personalized preparation of selective laser sintering 3D printing is supported, and the requirements of different clinical scenes are met.

The invention discloses a magnesium oxide coaxial electrospun bone biological material tensile strength and elongation detection device, and belongs to the technical field of material detection.The magnesium oxide coaxial electrospun bone biological material tensile strength and elongation detection device comprises a clamping mechanism, loading mechanisms are connected to the two sides of the clamping mechanism, the device further comprises a detection mechanism for detecting the elongation, and the clamping mechanism comprises a first side plate and a second side plate; the lower side of the first side plate is rotationally connected with a guide rod, a guide sleeve is rotationally arranged on the second side plate, and the guide rod is slidably inserted into the guide sleeve; a first bearing plate and a second bearing plate are arranged on the first side plate and the second side plate, a first sliding way and a second sliding way which are symmetrically arranged are arranged on the first side plate and the second side plate correspondingly, a pressing rod is slidably connected into the first sliding way and the second sliding way, a first pressing block is fixedly connected to the lower side of the pressing rod, and a second pressing block is slidably connected to the pressing rod. And the upper end of the pressing rod is connected with a hydraulic driving rod. The device has the advantages of fast clamping and multi-angle composite loading.

The invention discloses a preparation method of a micron-sized cell-matrix co-polymer biological material. The preparation method comprises the following steps: firstly, inducing SHED cells in an ultra-low adsorption plate by using an alpha-MEM culture medium containing serum, GlutaMAXI, vitamin C and the like to form micron-sized spheres (SHED spheres); secondly, taking human dentin, and carrying out mechanical crushing, EDTA (Ethylene Diamine Tetraacetic Acid) demineralization, grinding and sterilization to prepare 1-100 microns of dentin matrix particles (DDMPs); and finally, by taking the GelMA hydrogel as a carrier, entrapping SHED spphere and DDMPs, and carrying out 405nm photo-crosslinking for 30 seconds to form the hydrogel. The co-polymer can significantly promote angiogenesis and osteogenesis coupling regeneration in a bone defect model, and is suitable for oral cavity and cranio-maxillofacial bone repair.

The utility model discloses an absorbable net-shaped device for fixing bone grafting materials, and relates to the technical field of medical oral cavity, the absorbable net-shaped device comprises a woven net and bone nails, the woven net is made of absorbable biological materials, tightening ropes are arranged at four corners of the woven net, and each tightening rope is correspondingly wound on one bone nail. The bone nail is rotationally nailed into the dentale, the woven net is tightened to fix the bone grafting material, meanwhile, the woven net is an absorbable biological material and does not need to be taken out through a special operation in the later period, and the pain of a patient and the medical burden are greatly relieved.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The invention relates to the technical field of oral biological materials, in particular to a self-adaptive anti-caries coating as well as a preparation method and application thereof. The coating is prepared from random and block polymers, and is specifically prepared by polymerizing acid radical-containing functional monomers, phenylboronic acid and derivatives thereof. Construction of the coating depends on specific coordination of a functional group and Ca < 2 + > in enamel, and stable anchoring on the tooth surface is achieved. The core action mechanism is to introduce phenylboronic acid groups to endow the coating with pH sensitive characteristics: in a cariogenic acid environment, biofilm formation is synergistically inhibited through a plurality of mechanisms such as physical barrier, electrostatic repulsion and inhibition of mucoprotein-mediated adhesion. According to the application, the Ca < 2 + > anchoring monomer, phenylboronic acid and the derivative thereof are copolymerized, design is carried out aiming at two key links of protein adsorption and bacterial adhesion, multi-mechanism synergy and environment dynamic response are integrated, and an integrated anti-caries effect from early intervention to long-term protection is realized.

The invention discloses a preparation method and application of a mitochondrial targeting polypeptide modified carbon quantum dot probe, and relates to the technical field of nano biological materials and fluorescence imaging.The preparation method comprises the steps that citric acid and urea are dissolved in a dimethyl sulfoxide solution, mixed, heated, filtered, added with ethyl alcohol, centrifuged and freeze-dried, and carbon quantum dots are obtained; adding an MES buffer solution and an activating agent, reacting the polypeptide at room temperature, heating, reacting, centrifuging, dialyzing and freeze-drying to obtain polypeptide modified carbon quantum dots; the carbon quantum dots are synthesized from bottom to top, and the preparation method is simple. After polypeptide modification, the product has an obvious mitochondrial targeting effect, and the product is good in biocompatibility, so that the product can be used for imaging and dynamic tracking of living organelles. The probe developed by the invention shows obvious application potential in key cell events such as mitochondrial dynamic monitoring and the like.

A functionalized tamarind seed polysaccharide, and a process for its preparation. In particular, said functionalized tamarind seed polysaccharide is a TSP having a selected functionalization degree and molecular weight, showing improved mucoadhesive properties, as well as stability and compatibility with pharmaceutical ingredients. Therefore, the invention also relates to a pharmaceutical composition, as well as a biomaterial, comprising said f-TSP.

The application discloses a kind of magnetically responsive hydrogel optical fiber and its preparation and application, belong to medical biomaterial field.The optical fiber includes from inside to outside: polyethylene glycol diacrylate and acrylamide photocrosslinking light transmission core layer;Calcium alginate low refractive index skin layer;Doped nano-magnetic response layer of ferriferrous oxide;And polydopamine grafting polyvinylpyrrolidone lubricating layer.It is prepared by coaxial extrusion and light / calcium step-by-step crosslinking.The structure realizes the spatial isolation and anti-interference of light transmission and magnetic drive, has low background light loss and anti-bending stability, has biomechanics matching, ultra-low friction and magnetic field accurate active navigation ability, solves the pain point that extremely soft optical fiber is difficult to directional intervention.Suitable for deep tissue precise photothermal therapy, in vivo continuous optical sensing and minimally invasive diagnosis and treatment integration and other frontier medical fields.

By means of the method according to one aspect, in a process of enhancing biocompatibility of an acellular matrix by supercritical carbon dioxide treatment, immunogenicity of the acellular matrix can be reduced with effective efficiency while maintaining a content of a physiologically active substance and physiologically active ability. Accordingly, the acellular matrix prepared by the method according to one aspect, the composition including the same, and the biomaterial have low biotoxicity and excellent tissue regeneration energy, and thus can be usefully used in cosmetic or medical materials.

The application discloses a multifunctional biomaterial with light protection and repair functions, which comprises the following components in percentage by mass: 4-8% of methacrylated gelatin, 0.5-2% of nanomicelles of ferulic acid, 0.5-1% of adhesive peptides, 0.5-1.5% of hyaluronic acid and 0.5-1% of ekdromicin. The application further discloses a preparation method of the multifunctional biomaterial and application thereof. The multifunctional biomaterial realizes a trinity synergistic effect of 'protection-repair-regeneration', target enrichment of active ingredients to damaged parts, and can stimulate vascular regeneration, improve skin microcirculation, has antioxidant, anti-inflammatory and healing functions, and promotes repair of light-damaged skin.

This invention discloses a method for preparing and applying a shell-core composite flexible biomaterial. The flexible biomaterial consists of an outer PMMA electrospun layer, a middle PTFF or PE layer, and a core PEG hydrogel; both the PMMA electrospun layer and the middle layer are porous. This flexible material is soft and elastic, exhibiting good film-forming and osteogenic induction effects. Animal experiments have confirmed that, compared to traditional bone cement, the flexible composite bone cement-induced biofilm shows an increase in CD31-positive cells (representing angiogenesis) and M2 macrophages (representing tissue repair), exhibiting better film-forming properties. It is a promising composite biomaterial that can be widely applied in the treatment of soft tissue defects, bone defects, and bone infections, achieving better therapeutic effects.

An anti-CD122 antibody, and the preparation and use thereof. Provided is a CD122 antibody or an antigen-binding fragment thereof, comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises HCDR1-3 as set forth in SEQ ID NOs: 8-10, and the light chain variable region comprises LCDR1-3 as set forth in SEQ ID NOs: 11-13. Further provided are a biomaterial, pharmaceutical composition, kit and use related to the CD122 antibody. The antibody can effectively bind to a CD122 antigen and a cell expressing CD122 on the cell membrane, and can block the interaction between the cell expressing CD122 on the cell membrane and IL-15 or IL-2.

The invention provides a high-porosity and anti-adhesion tendon repair material, and relates to the field of medical biomaterials.The high-porosity and anti-adhesion tendon repair material is prepared through the following steps that animal tendons are obtained, degreased and rinsed; decellularization and rinsing are carried out; performing disinfection; performing rinsing; stretching and freeze-drying: clamping two ends by using a clamp, stretching and fixing, and freeze-drying to form pores in the tendon; soaking adsorption: taking out the freeze-dried tendon, putting the tendon into a tubular mold, pouring the anti-adhesion hydrogel precursor solution into the tubular mold, and performing soaking treatment for 12-24 hours; and performing irradiation crosslinking: performing irradiation treatment on the tubular mold filled with the tendon and the anti-adhesion hydrogel precursor solution to obtain the tendon repair material. The tendon repair material not only can effectively prevent the tendon from adhering to normal tissues, but also can control the pore size of the tendon, is beneficial to infiltration and proliferation of fibroblasts and tendons, promotes blood vessel ingrowth and metabolite exchange, and provides a good basis for tissue repair and regeneration.

The application discloses an anticoagulant biomaterial for blood purification and a preparation method and application thereof, and comprises the following steps: providing an amphiphilic copolymer P(AA-MMA); blending polyethersulfone, P(AA-MMA) and a solvent, stirring at a set temperature to obtain a casting solution, and preparing a blending membrane from the casting solution; soaking the blending membrane in a 2-morpholine-4-yl ethanesulfonic acid buffer solution containing argatroban, 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide and hydroxy succinimide, and reacting at room temperature to obtain an argatroban grafted blending membrane, namely the anticoagulant biomaterial. The method improves the hydrophilicity of the membrane, reduces the albumin adsorption of the membrane, reduces the adhesion of platelets on the membrane, prolongs the coagulation time APTT, PT and TT of the membrane, and is better in biocompatibility and enhanced in anticoagulation performance.

The application relates to the field of biomaterials, in particular to a preparation method of injectable acellular matrix powder and application thereof. The preparation method of the injectable acellular matrix powder is obtained through two discontinuous crosslinking steps of once crosslinking and twice crosslinking after crushing of acellular basement membrane. The obtained injectable acellular matrix powder with a unique crosslinking network structure is adapted to the natural skin pH value, greatly enhances the biocompatibility, simultaneously inhibits excessive inflammatory reaction and accelerates tissue healing through the induction of M2 polarized macrophage activation, effectively relieves the local tissue compression risk possibly caused by high cohesive force due to amino acid modification and introduction of a double crosslinking agent, realizes dynamic balance of mechanical stability and physiological friendliness, continuously stimulates the generation of skin collagen by using controllable moderate inflammatory reaction, and promotes gradual reconstruction and improvement of tissues.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The application belongs to the technical field of medical biomaterials, and particularly relates to an interpenetrating network polymer based on phosphorylcholine polymer and a preparation method and application thereof. The application provides a sequential interpenetrating network method, which comprises the following steps: preparing a monomer infiltration solution containing phosphorylcholine monomer, polyethylene glycol methacrylate, dimethylaminoethyl methacrylate, butyl methacrylate, hydroxyethyl methacrylate, ethylene glycol methacrylate, a crosslinking agent and an initiator; dipping a high-molecular polymer matrix into the monomer infiltration solution, and performing ultrasonic oscillation treatment to promote the infiltration of monomers into the interior of the matrix; and performing a polymerization reaction to obtain a final product after washing; the material prepared by the application has excellent thermal stability, excellent swelling performance, intelligent pH response drug release capacity and biocompatibility, and is suitable for the fields of biological medicine and cosmetics.

This invention relates to the field of metal nanomaterials technology, and discloses a MgTA@MnO2 nanozyme, which uses manganese dioxide nanoparticles as the core, with a metal phenolic network coating formed by the coordination of tannic acid and magnesium ions on the surface of the manganese dioxide nanoparticles; its preparation method; and the application of the MgTA@MnO2 nanozyme in the preparation of biomaterials for bone tissue repair or regeneration. This invention is the first to propose a nanozyme with manganese dioxide nanoparticles as the core, coated with a metal phenolic network coating formed by the coordination of natural polyphenols tannic acid and magnesium ions on the surface of the MnO2 core. This provides a multifunctional integrated bioactive material that can simultaneously and synergistically address the complex pathological microenvironment of bone repair. The overall process is simple, the conditions are mild, no complex equipment is required, it has good reproducibility, and it is easy to scale up production.

The invention discloses an instant plugging agent as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The instant plugging agent comprises a solution A and a solution B, wherein the solution A is an organic solution containing lipoic acid; the solution B is an aqueous solution containing metal ions. The preparation method comprises the following steps: dissolving lipoic acid in an organic solvent to prepare a solution A; dissolving salt of metal ions in water to prepare a solution B; the application refers to the application in preparation of products for stopping bleeding of human bodies or animal bodies and / or sealing tissue defects. The plugging agent has the characteristic of instant curing, and can tolerate bursting pressure exceeding 450 mm Hg; the device is not only suitable for general wound errhysis, but also can effectively deal with acute hemorrhage and emergency plugging of tissue defects in high-pressure and high-flow-rate states.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.