1759 results about "Biomaterial" patented technology

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

Provided are a salt tolerance related protein GmXTH32 having an amino acid sequence as shown in sequence 2, and a related biomaterial and use thereof. The use comprises: improving salt tolerance of plants and / or preparing products for improving salt tolerance of plants; and improving the survival rate of plants under salt stress and / or preparing products for improving the survival rate of plants under salt stress. The salt tolerance of plants can be changed by regulating and controlling the expression level of a coding gene of the protein in the plants, and the salt tolerance of the plants can be improved by reducing the expression level of the coding gene of the protein.

The invention encompasses innovative AI-powered platforms that predict various parameters (e.g. compostability, sustainability, material mechanical properties, formulate solutions properties, economic factors, etc.) and generates formulations for sustainable materials, while accounting for the various physical and non-physical properties as wells the applications they serve. The invention encompasses systematic search algorithms and / or machine learning techniques for identification of biomaterials that are predicted to have applicable properties. This invention encompasses a powerful method and tool to streamline the design and development of materials that are sustainable. In various embodiments, by employing advanced data processing, polymer chemistry knowledge and predictive modeling, the embodiments of the present invention provides precise and efficient recommendations, enhancing the research and development of sustainable biomaterials. Leveraging evolutionary and machine learning methodologies, the embodiments of the present invention identify polymer materials that adhere to real-world renewable constraints and other parameters, emphasizing a multitude of pertinent physical and non-physical properties.

The invention belongs to the technical field of biological material engineering, and particularly relates to a fungal imitation leather material and a preparation method thereof. The method comprises the following steps: firstly, adopting wild fomes fomentarius subjected to 60Co irradiation mutagenesis as a production strain of a mycelium pad, and screening and optimizing components and content of a liquid culture medium to obtain a surface culture liquid culture medium suitable for the wild fomes fomentarius; secondly, by utilizing the strain and an optimized culture medium, the low-cost and large-scale production of the mycelium pad can be realized under the optimal culture condition; and the mycelium pad is subjected to various physical and chemical treatments such as plasticizing, cross-linking, hot-pressing compaction and the like, so that the mechanical strength of the mycelium pad is further improved, and the mechanical property requirements of leather products are met.

The invention provides cat serum albumin and application thereof, and relates to the technical field of protein engineering. According to the invention, mutation is carried out on the cat serum albumin for the first time to obtain a beneficial mutation site, so that the cat serum albumin mutant which can be highly expressed in a pichia pastoris system is obtained. In addition, the cat serum albumin mutant I31L also has the effect of treating ascites due to cirrhosis, and the treatment effect is obviously higher than that in the prior art. A new biological material is provided for application of the cat serum albumin, and a foundation is laid for genetic engineering modification of the cat serum albumin.

The invention discloses a recombinant XVII type collagen engineering bacterium as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering, and the recombinant XVII type collagen engineering bacterium is realized through three steps of designing and optimizing collagen, improving translation through codon optimization and selecting a vector for cloning. By optimizing the amino acid sequence and the codon, the expression efficiency and the stability of the recombinant XVII type collagen in engineering bacteria are improved. The optimization not only improves the production efficiency of the protein, but also reduces the production cost. The recombinant XVII type collagen has potential in the medical field, especially in the fields of skin repair, wound healing, aging resistance and the like. Meanwhile, in the fields of beauty and biological materials, collagen is widely applied to products such as masks and skin care products as a basic component, and can promote skin cell regeneration and enhance skin elasticity and repair capacity.

The invention provides a catechol functionalized gelatin microsphere and application of the catechol functionalized gelatin microsphere in preparation of a product for inhibiting formation of subcutaneous effusion and promoting tissue repair, and relates to the technical field of biological materials.According to the designed and prepared catechol functionalized gelatin microsphere, catechol groups are introduced into the gelatin microsphere, and the catechol functionalized gelatin microsphere is prepared; good liquid absorption performance and biodegradability are realized. Cell experiment results show that the catechol functionalized gelatin microspheres have super multiple interfaces and can promote cell adhesion and proliferation, and the loose structure of the catechol functionalized gelatin microspheres can promote cell migration. In-vivo rabbit chest wall defect repair experiment results show that the catechol functionalized gelatin microspheres not only can inhibit formation of subcutaneous effusion, but also can collect autologous cells in situ and promote proliferation of the autologous cells and secretion of extracellular matrixes (such as collagen). The results show that the catechol functionalized gelatin microsphere material has clinical application potentials of inhibiting formation of subcutaneous effusion and promoting healing of subcutaneous wounds.

The invention provides a recombinant humanized elastin with high biological activity as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering and biological materials. 100% of the amino acid sequence of the elastin is derived from human elastin and is prepared by expression of genetically engineered bacteria; the protein has a typical beta-spiral hydrophobic structural domain and a lysine-rich hydrophilic structural domain, has a good bionic structure and functional characteristics, can significantly promote proliferation, adhesion and migration of human skin fibroblasts, also has excellent antioxidant, anti-wrinkle and anti-aging activities, and can be used for preparing a biomimetic protein. The hydrogel can be widely applied to the fields of skin repair dressings, injection implants, artificial skin, biomedical materials, functional skin care products and the like; in addition, the elastin is good in solubility, high in stability, mature in expression system and suitable for large-scale production.

The invention discloses a metal ion-mediated composite polysaccharide-based steady-state probiotic loading body as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. Sodium alginate and metal ions are coordinated and self-assembled through interaction of non-covalent supramolecules to form a metal ion-alginate skeleton network, functional guar gum is further filled, nano-scale aggregates are pushed to be spontaneously deposited on the surfaces of thalli, and a compound polysaccharide coating with excellent adhesion performance and steady-state performance is generated, so that the stability of the composite polysaccharide coating is improved. The independent coating of the probiotics is realized. The presence of metal ions increases the affinity of the biological material to the coated probiotics, and guar gum endows the probiotic delivery system with good adhesion characteristics. According to the probiotic loading body, the operation steps of an existing cell surface modification technology are simplified, the density is improved while the usage amount of biological materials is reduced, cells can be protected from being damaged by the gastrointestinal tract stress environment so as to maintain the activity of probiotics, and the colonization capacity and the biological activity of the probiotics in the intestinal tract are improved.

The invention provides a drug screening and curative effect evaluation method and system based on a glioma organ-like model, and relates to the technical field of biological material analys.The method includes the steps that a three-dimensional fluorescence image of the glioma organ-like model is collected, signal intensity analysis and compensation processing are carried out, a growth feature mapping model is constructed, and the three-dimensional fluorescence image of the glioma organ-like model is obtained; and phenotypic characteristics for representing the glioma organoid heterogeneity are obtained. Inputting the phenotypic characteristics into a drug response evaluation network, and calculating a drug sensitivity score; the drug molecular feature library and the drug collaborative analysis model are combined, the synergistic interaction index of different drug combinations is calculated, the optimal drug combination is screened, the drug administration dosage and the drug administration time sequence are determined in combination with phenotypic features, and finally an individualized treatment scheme is generated. According to the invention, the curative effect of the drug on the glioma organs can be effectively evaluated, and the optimal drug combination and administration scheme can be screened out, so that the accuracy and effectiveness of glioma treatment are improved.

A system for preparation of an implant and ab interno insertion of the implant into an eye including a handle having one or more actuators and an elongated shaft having an outer sheath and an elongate member positioned within a lumen of the tubular outer sheath. The system includes a recess sized for holding a patch of material fixed relative to the handle and a cutting member movable relative to the handle and to the recess. The cutting member cuts the patch of material into an implant as the cutting member moves towards a cutting configuration. The implant, once cut, is axially aligned with the lumen of the tubular outer sheath. The inner elongate member is movable relative to the tubular outer sheath to advance the implant into a deployment position in the lumen of the tubular outer sheath for delivery into the eye. Related devices and methods are provided.

The present disclosure discloses a limb bone fixation system capable of automatically transforming from AO (Association for Study of Internal Fixation) to BO (Bio-logical Osteosynthesis) elastic fixation. The system includes a bone nail, a bone bridge, and connecting pieces attached to the bone bridge. The bone bridge is divided into a plate-shaped bone bridge and a rod-shaped bone bridge. A gasket made of a rigid degradable biomaterial is added between all connecting pieces at a broken end of one side of a fracture and the bone bridge, which can form rigid fixation for the fracture at initial fixation.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of droplet digital nucleic acid detection and biological materials, in particular to microgel based on acrylic anhydride gelatin and nucleic acid to be detected as well as preparation and application of the microgel. The preparation method comprises the following steps: firstly, dissolving a photoinitiator and acrylic anhydride gelatin, and uniformly mixing to obtain a pre-polymerized solution; uniformly mixing the pre-polymerized solution with a to-be-detected nucleic acid mixed solution to obtain a dispersion phase; oil incompatible with the dispersed phase is used as a continuous phase; building a constant-temperature device, and enabling the dispersed phase to form liquid drops by using a liquid drop microfluidic method; and finally, performing amplification treatment on the liquid drops, and then performing photo-initiation crosslinking to obtain the microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected. The prepared microgel based on the acrylic anhydride gelatin and the nucleic acid to be detected is further applied to detection of target nucleic acid.

Provided are a self-crosslinking recombinant humanized collagen polymer biomaterial and a preparation method therefor. Provided is a collagen self-assembled element, comprising (i) at least 21 consecutive amino acids at the C-terminus of the amino acid sequence as shown in SEQ ID NO. 1; or an amino acid sequence that is obtained by means of substitution, deletion or addition of one or more amino acids in the amino acid sequence as described in (i) and that retains the function of promoting the self-crosslinking of recombinant type III humanized collagen to form a triple helix structure; or an amino acid sequence that has at least 90%, 92%, 95%, 96%, 97%, 98% or 99% identity to the amino acid sequence as described in (i) and that retains the function of promoting the self-crosslinking of recombinant type III humanized collagen to form a triple helix structure. The element can effectively promote the self-crosslinking of recombinant type III humanized collagen to form a triple helix structure.

The present invention discloses a method for extracting a stable body from cyanobacteria, comprising the following steps: S1. Cultivating cyanobacteria; S2. Pretreating cyanobacteria; S3. Ultrasonic disruption; S4. Differential centrifugation; S5. Ultrafiltration; S6. Ultracentrifugation; S7. Density gradient centrifugation; S8. Removing peripheral proteins. The extraction method provided by the present invention has high extraction efficiency, stable and controllable process, no toxic by-products, is green and environmentally friendly, and does not require high temperature and high pressure equipment and large instruments and equipment, thereby expanding application scenarios and reducing application costs. The present invention can obtain high polyP n Stable, active granular biomaterials with high polymerization degree, concentration, and purity, as well as high bioactivity, can meet the stringent requirements of applications in the food and daily chemical sectors, as well as clinical medicine and scientific research. They possess significant practical application value and commercial prospects, and will address the technical challenges of preparing stable, active granular biomaterials in China.

The invention relates to the technical field of biological materials, in particular to silver nano-chlorogenic acid composite hydrogel as well as preparation and application thereof. According to the invention, the CGA and the silver nitrate are used for green synthesis of the nano-particles CGA-AgNPs, and the nano-particles have peroxide nano-enzyme activity and antibacterial activity; and the CGA-AgNPs and chitosan (CS) are cross-linked to form the silver nano-chlorogenic acid composite hydrogel CGA-AgNPs-CS, so that the uniform distribution of nano-particles in the hydrogel can be promoted. According to the invention, multi-functionalization is taken as a target, a novel hydrogel composition system is designed from the source, the provided preparation method is green, simple, rapid and environment-friendly, the obtained composite hydrogel has multiple functions, and a good foundation is laid for expansion and application of the composite hydrogel in the biomedical fields of wound repair, wearable equipment, nano antibacterial drugs and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a morinda officinalis-synanthrin composition and application thereof in renal fibrosis products. The radix morindae officinalis-synanthrin composition provided by the invention is prepared from radix morindae officinalis polysaccharide, synanthrin and water, the synergistic effect of the two polysaccharides can be exerted, the physical and chemical properties and biological activity of the radix morindae officinalis-synanthrin composition can be improved, the metabolic rate of the kidney to urea nitrogen creatinine can be remarkably increased, the fibrosis positive area can be remarkably reduced, and the bioavailability of the radix morindae officinalis-synanthrin composition is improved. The traditional Chinese medicine composition has a remarkable effect on treating renal fibrosis diseases. The raw materials of the composition are based on natural polysaccharides, so that the composition has good safety and degradability, and meets the high requirement of the medical field on the safety of biological materials. Compared with an existing single component, the compound design can achieve the multi-target effect, the treatment effect is remarkably improved, and the compound medicine has the large market competitive advantage.

The application discloses a kind of imitated plant leaf hydrogel composite material and preparation method thereof, belong to bionic composite material field.The application first by colorant compounding preparation printing paste, and fabric is printed, obtain the green fabric that can realize to plant leaf " green peak " and " red edge " spectral characteristic simulation, and with green fabric simulation leaf structure in fence organization and skeleton;Then with biomaterial polyvinyl alcohol (PVA) as base material, join with strong hygroscopic monomer and crosslinking agent, initiator blend preparation hydrogel prepolymer solution;Finally, hydrogel prepolymer solution is poured into the mold containing green fabric and copolymerization, it directly forms hydrogel in fabric surface and pore interior, obtains imitated plant leaf hydrogel composite material.The material gets rid of the dependence on hygroscopic salt, has water absorption and simulation effect stable, durability is good and the like advantages.

The invention discloses an extracellular matrix-imitated anti-inflammatory nerve repair-promoting conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of high polymer materials. The preparation method of the conductive hydrogel comprises the following steps: preparing an MXene nanosheet dispersion liquid, and sequentially adding polyphenol and magnesium ions into the MXene nanosheet dispersion liquid to obtain a modified nanosheet solution; mixing the modified chitosan with polyvinyl alcohol to obtain a solution A; mixing and reacting the modified nanosheet solution with phenylboronic acid modified gelatin to obtain a solution B; mixing the solution A and the solution B for reaction, refrigerating at low temperature, and unfreezing to obtain the conductive hydrogel. The hydrogel prepared by the invention has excellent conductivity, and the obtained conductive hydrogel has the functions of resisting inflammation and promoting nerve repair. The bionics method not only improves the diversity and functionality of biological materials, but also provides new possibility for future tissue engineering and regenerative medicine.

The invention belongs to the technical field of biological materials and ultrasonic printing, and particularly discloses a biological adhesive for ultrasonic printing and preparation and application thereof. The biological adhesive comprises a heat-sensitive main body material and a chemical adhesion component, the heat-sensitive main body material comprises hydroxybutyl chitosan, and the chemical adhesion component comprises polyacrylic acid-N-hydroxysuccinimide ester. The bioadhesive has excellent ultrasonic responsiveness, formability and bioadhesion performance, directional deposition, in-situ solidification and tissue surface adhesion can be achieved in a non-contact and accurately-controlled ultrasonic printing system, and the bioadhesive is suitable for various biomedical scenes such as tissue repair, minimally invasive medicine, biological device construction and drug delivery interface printing.

The invention discloses photo-thermal conversion wood-based hydrogel and a preparation method thereof in the technical field of nano materials and biological materials, and the preparation method comprises the following steps: synthesizing a conjugated polymer CP, dissolving the CP in THF, and adding DSPE-PEG2000 and hemin to obtain CP-Hemin nano powder; and compounding the CP-coated Hemin nano powder, flexible wood and PVA (Polyvinyl Alcohol) to obtain the wood-based hydrogel. The wood-based hydrogel provided by the invention has high photothermal conversion efficiency, can be rapidly heated under laser induction of a near-infrared second region, releases reactive oxygen free radicals at the same time, can be applied to photothermal therapy and photodynamic therapy, and has a prospect of being applied to the fields of catalysis, medical treatment and the like.

The present invention relates to the field of medical biomaterial technology, and particularly, to an in-situ formed injectable hydrogel material and use thereof in preparing a tissue repair formulation. The in-situ formed injectable hydrogel material of the present invention comprises two fluid components: component A and component B, wherein component A is an activated linker solution, and component B is a collagen and / or gelatin fluid. The hydrogel material of the present invention is prepared during use. First, a cross-linking activator is used to activate the linker to give component A. During this process, a portion of the cross-linking activator is consumed, thus reducing the amount of cross-linking activator in contact with tissues. Subsequently, component A and component B are mixed, and the macromolecules in the components undergo chemical cross-linking to form a gel. A neutralizing agent is introduced into component B to consume the cross-linking activator in the material during the chemical cross-linking of the macromolecules and a period thereafter, so as to further reduce the residual amount of cross-linking activator in contact with tissues and enhance the biocompatibility of the hydrogel material.

The invention provides a high-modulus strong-adhesion injectable hydrogel, and belongs to the field of biological materials, the hydrogel is the injectable hydrogel with the highest modulus at present, the modulus is up to 10.7 MPa, the modulus is adjustable, the adhesion strength also reaches 160kPa, and the hydrogel can well simulate tissues with different moduli such as skin and cartilage; meanwhile, the hydrogel can also resist swelling and lubricate. The invention provides a preparation method of the hydrogel, which comprises the following steps: mixing a silk protein solution of the wild silkworm silk and the modified polysaccharide in proportion, pre-crosslinking, and further crosslinking by utilizing temperature, so as to obtain the hydrogel with high modulus and strong adhesion. According to the hydrogel, firstly, fibroin and polysaccharide are mixed to form water-insoluble fluid gel, the water-insoluble fluid gel can be used for underwater injection in arthroscopic surgery, and then body temperature triggers assembly to form tough hydrogel in situ. In addition, the invention also proves that the hydrogel can be used for suture-free skin repair and can effectively avoid wound disintegration.

Biocompatible and biodegradable polymers are provided herein as compositions, methods, and systems for making the compositions. These polymers can be adjustably stable and used in medical applications as adhesives and sealants, and in non-medical applications. Radical polymerization protocols, and two-step cationic and radical polymerization protocols, are provided for the creation of stable, polydisulfide polymers, which can be done in aqueous solution and at ambient and in vivo temperatures ranging, for example, from 0° C. to 37° C. The compositions are biocompatible and can be used as fast curing, biocompatible and biodegradable adhesives and sealants suitable for use in the consumer, industrial, military and medical arts, namely as a functional biomaterial, sustainable plastic, surgical superglue, bone adhesive, pressure-sensitive adhesive, structural adhesive, and other relevant sustainable materials with closed-loop recycling.

The invention relates to the field of biological materials, in particular to a thioketal bond modified 17-type collagen dopamine drug-loaded nanoparticle composite microneedle patch and a preparation method and application thereof, and the microneedle patch is composed of dopamine drug-loaded nanoparticles, thioketal bond modified recombinant 17-type collagen and a polymer substrate. The thioketal bond modified 17-type collagen and polyvinyl alcohol are subjected to physical and chemical double-crosslinking to form hydrogel, then the hydrogel is loaded with drug-loaded nanoparticles, and the hydrogel and a polymer substrate form the ROS and pH value microenvironment double-response collagen microneedle patch loaded with bioactive substances. When the collagen hydrogel is applied to the skin, the tip of the microneedle reaches the area where the hair follicle stem cells exist in a painless and minimally invasive mode, and due to the change of microenvironment molecules in the skin tissue, the breakage of thioketal bonds in the collagen hydrogel can be promoted, so that active oxygen is removed, and the wrapped IGF-1 is released into the hair follicle stem cells; and a potential treatment strategy is provided for androgenetic alopecia.

The invention discloses a preparation method and application of multifunctional antibacterial gel, and relates to the technical field of biomedical injectable hydrogel materials with antibacterial activity. The cationic guar gum (CG) is used as a cross-linked network and is loaded with a water-soluble Fe (II)-based metal organic metal cage (Fe-1SO4), and through in-situ chemical dynamic therapy (CDT) and photodynamic therapy (PDT) induced under irradiation of 660 nm laser, rapid healing of surface wounds is promoted through synergistic antibiosis. Fe-1SO4 is a water-soluble metal cage which takes Fe (II) as a metal node and 5, 10, 15, 20-tetra (4-aminophenyl)-21H, 23h-porphyrin (TAPP) as an organic ligand, and can be quickly degraded within 5 days in a biological environment. According to the present invention, with the application of the fenton-like reaction in the preparation of the antibacterial agent, the obvious PDT effect can be provided under the irradiation of 660 nm laser so as to generate a large amount of singlet oxygen (1O2), and the fenton-like reaction can convert H2O2 generated by bacterial infection into toxic hydroxyl radicals (OH) in situ, such that the excellent antibacterial activity can be provided in vivo and in vitro, and the wound healing can be promoted. The invention provides a simple strategy for the design of the rapidly degradable antibacterial hydrogel dressing, and is an important step for the clinical application of biological materials.

The invention provides bismuth oxyiodate composite piezoelectric hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel materials. The bismuth oxyiodate composite piezoelectric hydrogel disclosed by the invention is prepared from the following raw materials in percentage by mass: 0.125 percent to 0.5 percent of first self-assembled oligopeptide, 0.125 percent to 0.5 percent of self-assembled stem cell bone marrow homing peptide, 0 to 0.02 percent of BiOIO3 nanosheets and 98.99 percent to 99.75 percent of a solvent. BiOIO3NSs are integrated into self-assembled stem cell bone marrow homing peptide, the injectable and degradable piezoelectric hydrogel with ultrasonic responsiveness is constructed, BiOIO3NSs shows remarkable piezoelectric catalysis performance, generation of active oxygen is promoted, tumor cell apoptosis is induced, and the piezoelectric hydrogel has the advantages of being capable of being used for preparing the bone marrow homing peptide, capable of being used for preparing the bone marrow homing peptide and capable of being used for preparing the bone marrow homing peptide. The bismuth oxyiodate composite piezoelectric hydrogel disclosed by the invention is a biological material capable of inhibiting the growth and recurrence of tumors and recovering the structure and function of bones to the greatest extent.

The invention belongs to the technical field of biological medicine, and particularly relates to morinda officinalis polysaccharide-synanthrin composite gel as well as a preparation method and application thereof. The radix morindae officinalis polysaccharide-synanthrin composite gel provided by the invention is prepared from radix morindae officinalis polysaccharide, synanthrin and water. The radix morindae officinalis polysaccharide-synanthrin composite gel has good biocompatibility and multifunctionality, can intervene in the intestinal microenvironment and renal fibrosis at the same time, and fills the blank of existing treatment methods. The polypeptide has potential curative effects in the aspects of intestinal barrier repair, inflammation regulation and control, microorganism regulation and renal fibrosis inhibition, and a new treatment choice is provided for clinic. The raw materials of the composite gel are based on natural polysaccharides, have good safety and degradability, and meet the high requirements of the medical field on the safety of biological materials. Compared with the existing single-component hydrogel, the composite design of the hydrogel can realize a multi-target effect, the treatment effect is remarkably improved, and the hydrogel has a relatively great market competitive advantage.

The invention relates to the technical field of tissue engineering, in particular to a 3D printing platform integrated with holographic optical tweezers and a bionic tissue construction method.The printing platform comprises a holographic optical tweezers module used for forming an optical trap array in an objective lens focal region of the holographic optical tweezers module; the charging barrel is used for storing biological materials and can move between a storage position and a working position; the printing base can move between the feeding station and the optical tweezer control station; the objective table is installed on the printing base and can move in the horizontal direction and the vertical direction; the light curing device is fixed on the objective table and is used for emitting light to induce the biological material to be cured; the cell culture plate is fixed on the objective table and stacked on the photocuring device; the cell culture plate is provided with a printing chamber and a cell chamber, and the printing chamber and the cell chamber are communicated through a channel. A macroscopic ordered three-dimensional structure can be constructed, and ordering and patterning of microscale organisms in the macroscopic three-dimensional structure are also realized.