562 results about "Nanopore" patented technology

The invention relates to a non-linear seepage experiment method for gas breaking through a cover layer based on dark field imaging. The method comprises the following steps: placing a microfluidic rock chip on an objective table of a dark field scattering microscope; the injection end of the microfluidic rock chip is connected with a fluid injection module, and the outlet end of the microfluidic rock chip is connected with a downstream outflow pipeline; injecting the formation water solution into the micro-fluidic rock chip until the micro-fluidic rock chip is filled with the formation water solution; opening a gas injection system, and adjusting the dark field scattering microscope until a clear image of a micro-fluidic rock chip pore structure and carbon dioxide displacement formation water is obtained; injecting carbon dioxide with different pressures into the micro-fluidic rock chip, and observing nonlinear seepage migration images of carbon dioxide and formation water in the micro-fluidic rock chip; when it is observed that carbon dioxide migrates to the outlet end of the rock chip, the breakthrough moment is obtained, and the upstream and downstream pressure difference value of the microfluidic rock chip is the breakthrough pressure. According to the method, the nonlinear seepage of the carbon dioxide displacement formation water in the nanopores can be accurately observed, and the breakthrough pressure can be determined.

The invention discloses a nanopore sensor based on insect olfactory receptor protein as well as a construction method and application thereof, and belongs to the field of biosensors. According to the invention, the insect olfactory receptor protein is used as a sensitive element to be integrated into a nanopore measurement system, so that the nanopore sensor is obtained. By measuring a nanopore current signal, the prepared sensor is applied to detection of target odorant molecules. According to the method, the recognition capability of the insect olfactory receptor protein on odorant molecules and the single molecule level detection capability of the nanopores are utilized, expensive large experimental instruments and complex experimental operation steps are not needed, and sensitive, rapid and direct detection on odorant substances can be realized.

The invention relates to a variation identification method of a nanopore single molecule sequencing current signal, which comprises the following steps of: dividing an original current signal generated by nanopore single molecule sequencing into continuous time periods according to a sequence that a basic group passes through a nanopore, calculating an average signal rhythm, constructing a standard time rhythm when a standard basic group sequence passes through the nanopore, and identifying the variation of the nanopore single molecule sequencing current signal. Comparing the offset difference between the actually measured average signal rhythm and the standard time rhythm, wherein a difference salient region is a potential variation region; current signals of front and back neighborhoods are extracted, a current signal disturbance difference value is quantized, and a region with the disturbance difference value having obvious direction asymmetry characteristics is a variation region to be confirmed; the range is expanded, current signals are extracted, disturbance intensity is calculated, and when the disturbance intensity presents a gradually-enhanced or gradually-weakened continuous change trend, it is judged that the variation area to be confirmed has physical rationality; and determining a theoretical current signal by using the corresponding non-variation sequence, comparing the theoretical current signal with an actually measured current signal, calculating a current difference, and determining that the current difference exceeds a normal difference range as a real variation region.

The invention relates to a target molecule identification and quantification method based on a solid nanopore detection technology, electronic equipment and a computer readable storage medium. The method comprises the following steps: (1) acquiring current signal data generated by a target molecule through a nanopore sensor within a period of time; (2) carrying out filtering processing on the current signal data; (3) identifying the filtered data to obtain a target via hole event set; (4) filtering the target via hole event set, and extracting signal data of the filtered events to obtain a target molecule recognition event; and (5) calculating the average interval time of occurrence of the target molecule recognition event, and obtaining the occurrence frequency of the target molecule based on the average interval time, namely the relative concentration of the target molecule. According to the method, automatic identification and quantitative analysis are realized aiming at mass signal data generated by a solid-state nanopore detection technology, and the analysis efficiency and accuracy are improved by optimizing a data identification method.

The invention relates to the technical field of transmission membranes, in particular to a transmission membrane based on dithienylethene and a preparation method of the transmission membrane. The transmission film comprises a base film and light-operated molecules, the base film is coated with the light-operated molecules, and the light-operated molecules have light response groups. A detection result shows that after ultraviolet irradiation, the transmission film can quickly realize complete conversion from an open loop to a closed loop; after visible light irradiation is recovered, the transmission film can efficiently and reversibly recover to an open-loop state. In addition, the transport membrane has a high on-off ratio to five ions of sodium, potassium, lithium, magnesium and calcium. The transmission membrane based on dithienylethene, which is prepared by taking terminal amino-modified dithienylethene as a basic unit, not only has abundant nano-channels, but also is endowed with the characteristic of regulating and controlling the opening and closing of the nano-pore by a photoresponse functional group, and the closing and opening of an ion channel can be accurately regulated and controlled through the alternate irradiation of ultraviolet light and visible light, so that the transmission membrane has a good application prospect. And the controllability of light-operated ion transmission is finally realized.

The present invention relates to modified Dda helicases which can be used to control the movement of analytes such as polynucleotides. The modified Dda helicases are used in analyte detection and characterisation. The present invention also relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore.

A system for controlling a translocation speed of a molecule through a nanopore may include a fluid chamber containing a solution with a magnetic susceptibility that is different from the magnetic susceptibility of the molecule, a nanopore situated in the fluid chamber, and at least one magnetic component configured to create a magnetic field gradient within the solution to control the translocation speed of a molecule through the nanopore. A system for controlling a translocation speed of a molecule through a nanopore may include a nanopore at least one magnetic component situated to create a magnetic field that causes the molecule to experience a rotational torque as it passes through the nanopore.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sense a fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides.

The invention discloses a preparation method of a PVA / chitin porous nanosheet layered film for salinity gradient power generation, and belongs to the technical field of bio-based material functionalization. The preparation method comprises the following operation steps: firstly, preparing a chitin porous nanosheet by utilizing an acid steam combined ultrasonic strategy; modifying the surface of the chitin porous nanosheet with rich positive charges through quaternization reaction, and performing suction filtration and drying to obtain a porous nanosheet layered film; and finally, circularly freezing, thawing and packaging the porous nanosheet layered film by using polyvinyl alcohol hydrogel to prepare the PVA / chitin porous nanosheet layered film. According to the PVA / chitin porous nanosheet layered film, by means of combination of chitin nanosheet layer gap channels and micro-nano pore channels in nanosheet surfaces, ion transmission paths are increased, ion transmission resistance is reduced, the microstructure of the layered film is effectively stabilized jointly by means of the interlayer weak force effect and the hydrogen bond bonding effect between polyvinyl alcohol molecules, and therefore the film has the advantages of being capable of being used for preparing the composite film. And the optimal salinity gradient power generation output power density reaches 7.33 W / m < 2 >.

In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a cleavable cyclic loop between two positions of the nucleotide, wherein the cyclic loop comprises reporter moiety corresponding to the identity of the nucleotide. The reporter may be comprised of an amino acid sequence. The cyclic loop may further comprise modifications or arresting constructs for slowing or halting the polynucleotide translocation through a nanopore. Also disclosed are methods of detecting nucleotides and a kit for determining the sequence of nucleotides.

The invention provides a construction method of a bacterial pathogen detection model based on nanopore adaptive sampling and deep learning, and a method, equipment, a medium and a program product for detecting bacterial pathogens by applying the model, and relates to the technical field of nanopore sequencing. The bacterial pathogen detection method comprises the following steps: obtaining a water sample to be detected; and inputting a to-be-detected water sample into the detection model constructed in the invention to obtain a detection result of the to-be-detected water sample. The sensitivity and the accuracy of pathogen detection are obviously improved, and the improvement on prevention and control of water-borne diseases is facilitated.

The invention discloses an electrochemical biosensor of a light-driven nanopore channel and application of the electrochemical biosensor. According to the sensor, a photoresponse nanopore membrane is constructed, an outer surface aptamer probe is combined, asymmetric photothermal gradient driven ion transmission induced by near-infrared light is utilized, and ultra-sensitive detection on a target object is realized by regulating and controlling the charge density of the outer surface. According to the electrochemical biosensor provided by the invention, traditional voltage driving is replaced by asymmetric near-infrared light driving, so that the electrochemical biosensor has a relatively wide detection range and an extremely low detection limit, and high-sensitivity and specific detection on a target object can be realized. The electrochemical biosensor can be used for detecting microcystin-LR (MC-LR), the detection limit reaches 1 * 10 <-7 > mu g / L, the sensitivity is improved by 5 orders of magnitude compared with that of a traditional voltage-driven sensor, and the electrochemical biosensor can be applied to trace pollutant detection in the fields of environmental monitoring, food safety and the like.

The invention discloses a pathogenic microorganism detection system based on nanopore sequencing and intelligent interpretation. The system comprises six modules including a biomolecule electric signal acquisition module and a pathogen characteristic sequence extraction module. Ion current change signals are collected through a nanopore chip array, a pathogen characteristic sequence is extracted through differential coding, a hypergraph topological structure self-adaption building module is used for building a pathogen relation model, a multi-dimensional heterogeneous pathogen parameter fusion analysis module integrates morphological parameters, metabonomics parameters and other multi-dimensional parameters, and the multi-dimensional heterogeneous pathogen parameter fusion analysis module is used for analyzing the multi-dimensional parameters. The optimized hypergraph neural network reasoning module realizes pathogen category prediction, and finally, the nanopore sequencing result intelligent interpretation output module generates a detection report containing pathogen categories, subtypes and risk levels. The system is combined with nanopore sequencing and an intelligent algorithm, pathogenic microorganism detection can be efficiently and accurately completed, the accuracy, reliability and efficiency of detection are greatly improved, and the requirement for rapid and accurate detection of pathogens in practical application is met.

Provided herein are methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers having an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

A nanopore sensing device comprises a planar structure provided with plural fluidic passages extending between the first and second chambers. The planar structure supports nanopores in membranes across respective passages and sensor electrodes are arranged to sensea fluidic electrical potential in respective passages between the nanopores and the second chamber. The passages comprise planar fluidic resistor portions between the sensor electrode and the second chamber, the planar fluidic resistor portions extending in a planar direction of the planar structure and being configured to form a fluidic resistor.

Provided herein are methods of interrogating spatial gene expression in a sample using substrates having sequencing pores. The disclosed methods allow for spatial analysis of analytes from biological samples using a pore-based sequencing approach and without the need for a barcoded spatial array. In some embodiments, an analyte or intermediate agent thereof, is released from a biological sample and directly sequenced by traversing through pores of a sequencing array including a plurality of pores, e.g., nanopores. In some embodiments, light or other stimuli is used to release an analyte or intermediate agent thereof from a specific region of interest in the biological sample followed by sequencing using sequencing array including a plurality of pores.

Example systems and methods for de novo sequencing of DNA or DNA-like sequences using Viterbi-like correlation analysis are described. A sequencing system receives the read data for multiple copies of a DNA strand from a sequence reader, such as a nanopore reader. The sequencing system generates a convolutional matrix based on one copy and a reference matrix based on another copy and uses them to generate a correlation matrix. A most likely path through the correlation matrix is determined to identify and correct errors between the two copies.

The invention provides a biosensor based on heterostructure double holes and a preparation method and application thereof. The device comprises a silicon nitride chip fixed between two electrolyte-filled liquid storage tanks, a layer of graphene film is transferred on the silicon nitride chip in advance, one nanopore is etched on a graphene interface, and the other nanopore is etched on a silicon nitride interface. The two side liquid pools are respectively connected with the positive electrode and the negative electrode of the patch clamp amplifier. When a certain potential difference is applied to the two electrodes, the biological macromolecules in the liquid pool can be captured into the nanometer channel. When two ends of a molecule are captured by two different nanopores at the same time, due to different interaction between a silicon nitride-graphene heterogeneous interface and the molecule and the action of electric field force, the molecule can form a dragsaw phenomenon between the two pores, and the time of the molecule passing through the nanopores is prolonged. According to the invention, the rate of chain molecules such as DNA, protein and the like passing through the nanopore can be regulated and controlled, and accurate control of single molecules and even gene sequencing can be realized.

The invention provides a glass nanopore probe based on an aptamer and application of the glass nanopore probe in dopamine detection, and belongs to the technical field of electrochemical sensing and scanning probe microscopy. The sensor comprises: a glass nanopore probe, the inner wall of which is modified with a gold layer; a cDNA (complementary deoxyribonucleic acid) single chain is fixed on the gold layer through a gold-sulfur bond; a part of the sequence of the dopamine aptamer is hybridized and combined with the cDNA single chain; wherein the combination of the dopamine and the dopamine aptamer causes the change of an ion current rectification signal of the glass nanopore probe, so that the detection of the dopamine is realized based on the change of the ion current rectification signal of the glass nanopore probe. The invention provides a valuable scheme for developing an aptamer nanopore scanning electrochemical sensor for single cell analysis and researching neurotransmitter-related diseases.

The invention discloses a long-afterglow self-luminous material based on multi-element rare earth synergistic sensitization and a preparation method thereof. SrAlO is used as a matrix, trap level distribution is optimized through ternary rare earth synergistic doping according to the molar ratio of Eu to Dy to Nd being 1: (0.4-0.6): (0.2-0.4), and a ZnO nanorod array is grown on the surface of a light-emitting core in an in-situ epitaxial mode to improve the light extraction efficiency; and a hydrophobic modified SiO core-shell coating layer with an ordered mesoporous channel is combined, and oxygen diffusion is promoted by utilizing a nanopore channel so as to accelerate trap regeneration and synchronously inhibit non-radiative recombination. The afterglow time of the material is not less than 8 hours, and the afterglow retention rate after aging at 60 DEG C is not less than 80%. A solid-phase synthesis process and red mud / fly ash industrial solid waste raw materials are adopted, and the carbon emission intensity is smaller than or equal to 6 kgCO / kg. The material can be widely applied to the fields of green traffic infrastructures such as expressways and tunnels.

The invention discloses a species identification kit for animal mitochondrial bar code multiplex PCR amplification and nanopore sequencing, and relates to the technical field of species identification. The species identification kit comprises optimized primer pairs aiming at four hyper-variable genes (Cytb, COI, 16S rRNA and 12S rRNA) of mitochondria, by introducing R, Y, D and other degenerate bases at key sites, the binding capacity of the primers and template DNA of different species is remarkably improved, the problems that an existing universal primer is prone to mismatch, low in efficiency and even failed in amplification during cross-species amplification are solved, and the species identification kit has the advantages of being high in specificity, high in specificity and high in specificity. The method can be used for species identification of a mixed DNA sample containing at least two different animal species sources. The species identification primer combination is applied to preparation of an animal species identification kit, and the detection coverage range of the kit is expanded. According to the animal species identification method provided by the invention, a multiple PCR amplification system and a nanopore length reading sequencing technology are combined, congeneric sibling species with highly similar sequences can be distinguished, and the method has good accuracy.

Provided herein are methods and systems for sequencing proteins. One or more methods disclosed herein may use linkers having an amino acid-reactive group and an additional reactive moiety that may be used to couple a polymerizable molecule. The linker may couple to a polymerizable molecule and an amino acid of a peptide and a capture moiety via the polymerizable molecule, followed by cleavage of the amino acid from the peptide. Further processing and analysis may be conducted using, for example, nanopores or nanogaps.

A combination of lipid-binding molecules and / or lipid-binding proteins with lipid components (i.e., mispids) is provided for use in modifying the interaction between target molecules and lipid membranes. This includes, for example, the use of lipid-binding molecules and / or mispids to improve the sequencing efficiency and throughput of nanopore-based sequencing systems. To sequence target molecules such as nucleic acid sequences or nucleic acid substitute polymers derived therefrom, lipid-binding molecules and / or their mispids are combined with the target molecules. The mixture is then applied to a nanopore-based sequencing chip. The target molecules are then sequenced in the presence of lipid-binding molecules and / or nanodiscs, thereby improving the capture, arrival time, and effective concentration of the target molecules across the chip membrane. Such improved efficiency is particularly beneficial, for example, when the concentration of the target molecule is low.

The utility model discloses an integrated solid-state nanopore biological detector, which relates to the technical field of biological detection and comprises a fluid pool shell, a solid-state nanopore silicon nitride chip and a PCB (printed circuit board), a sample injection hole is arranged on the fluid pool shell, the solid-state nanopore silicon nitride chip is arranged in the fluid pool shell, and the solid-state nanopore silicon nitride chip is arranged in the PCB. A silicon nitride window corresponding to the solid-state nanopore silicon nitride chip is formed in the fluid pool shell, a PCB (Printed Circuit Board) is fixed in the fluid pool shell, an RFID (Radio Frequency Identification Device) storage chip, a magnetic connector and silver electrodes are arranged on the PCB, and the silver electrodes are positioned on two sides of the silicon nitride window. According to the utility model, the RFID storage chip is arranged, so that the key information of the biological detector is stored and traced, and the problem of information management disorder in the prior art is solved; the RFID storage chip can store key information of the biological detector for a long time, and the integrity and traceability of the data are ensured.

Provided is a method of manufacturing a microfluidic channel with a membrane formed therein, the method including: preparing an apparatus for forming a membrane, the apparatus for forming the membrane including a first microfluidic channel, a second microfluidic channel being spaced apart from the first microfluidic channel, a bridge channel having a microchannel structure for communicating the first microfluidic channel and the second microfluidic channel with each other, and a control channel, which is partitioned by a gas permeable member from the bridge channel and through which gas flows; a fluid flowing operation in which a first fluid in a liquid state for moving first microparticles flows in the first microfluidic channel and a control gas in a gaseous state flows in the control channel; and forming a membrane having nanopores.

To provide a system including novel nanopores having different structures and recognition sites that improve sequencing accuracy and / or provide different error profiles.SOLUTION: Provided are systems relating to the field of nanopores and their use in various applications, e.g., analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through the nanopore, comprising a funnel-shaped proteinaceous nanopore comprising alpha-helical pore-forming toxins that are members from the actinoporin family of proteins, more particularly Fraga Ceatoxin C (FraC), a variant FraC, a FraC paralog or a FraC homolog.SELECTED DRAWING: None

The invention relates to the field of microbiological detection, in particular to a microbiological detection and identification device and identification method.The microbiological detection and identification device comprises a main cylinder, a bearing plate is slidably arranged in the middle of the main cylinder, a plurality of circular grooves are formed in the bearing plate, storage units are placed in the circular grooves, and a puncture assembly is arranged in a lower cavity; one side of the puncture assembly extends into the circular groove and the storage unit and is used for supplementing an extraction reagent into the circular groove and the storage unit, a limiting assembly used for limiting and plugging the storage unit is arranged at the upper end of the main cylinder, and a nanopore tester connected with the puncture assembly is mounted and inserted in the bottom of the main cylinder; through mutual cooperation of the storage unit, the puncture assembly and the adsorption assembly, when a microbial sample is detected, microbial DNA and RNA nucleic acid separation can be efficiently achieved, nucleic acid is secondarily extracted through the adsorption assembly, the purity of the nucleic acid sample is accurately improved, and the detection efficiency is improved. The problems of low extraction purity and more impurities in the traditional method are solved.

The invention relates to means and methods for analysis of target analytes using nanopore-based sensors, more in particular to methods, nanopore systems and devices for single-molecule profiling of polymers, e.g. polypeptide or polysaccharides. Provided is a method for translocating a non-nucleic acid based polymer analyte through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising adding the analyte to the cis side of and allowing for translocation, wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a net ionic current flow cis to trans, preferably wherein the cis to trans EOF results from a net ionic current flow cis to trans over total ionic current flow of greater than 0.2 or less than −0.2.

The invention discloses a method for detecting polypeptide sequence difference and post-translational modification thereof based on a nanopore system, the nanopore system is constructed in the method, and the nanopore system comprises an alpha-hemolysin nanopore and octa-amino modified gamma-cyclodextrin embedded in the nanopore; the amino acid sequence of the alpha-hemolysin nanopore comprises mutation sites M113R and T145R, and the alpha-hemolysin nanopore is characterized in that the alpha-hemolysin nanopore According to the detection method, polypeptide with negative charges enters a nanopore under the driving of external voltage, an ion current blocking signal is recorded, and the polypeptide sequence difference and post-translational modification are analyzed according to blocking amplitude, duration, noise characteristics and event frequency. The method can detect a peptide chain with the length of 2-10 amino acid residues, can distinguish the post-translational modification state in homopolypeptide composed of 4-10 amino acids and polypeptide by single amino acid resolution, and can be used for protein enzyme digestion product analysis, oligopeptide drug detection and rapid polypeptide identification under the condition of no mass spectrum.