247 results about "Nucleic Acid Probes" patented technology

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The invention relates to the field of nucleic acid detection, in particular to a multifunctional dumbbell type nucleic acid probe, a kit, a target detection method and application thereof. The multifunctional dumbbell type nucleic acid probe provided by the invention comprises two or more of the following functional areas: a promoter area used for being combined with RNA polymerase; the target recognition area is used for recognizing and combining a target sequence and opening the dumbbell type nucleic acid probe; the dumbbell type nucleic acid probe can be transcribed and combined with a rolling ring, a specific sequence is subjected to mass transcription, amplification of the specific sequence is achieved, finally, signal amplification is achieved during detection, and the dumbbell type nucleic acid probe has the advantages of being simple in reaction system, low in background signal and high in detection specificity; and the dumbbell type nucleic acid probe can be designed according to different target sequences, so that the controllable detection of different targets can be realized.

The invention discloses a universal nucleic acid probe design method based on hybridization thermodynamics and kinetics, which comprises the following steps: 1) determining a target amplification region according to a fragment interval, generating a primer pair which meets the length requirement and covers a target fragment, calculating the Gibbs free energy change of nucleic acid hybridization by adopting an intelligent nucleic acid hybridization algorithm, and calculating the Gibbs free energy change of the target fragment according to the Gibbs free energy change; by combining chemical thermodynamics, kinetic parameters, sequence complementarity and enzymatic reaction characteristics, generating an optimal primer pair under a multi-dimensional constraint condition; and 2) on the basis of the generated optimal primer pair, generating a probe combination meeting a free energy difference threshold as a candidate pool, screening the probe combination with the highest objective function value from the candidate pool through an iterative process, and updating the candidate pool step by step until the primer pair and the probe combination with the highest objective function value are output. By introducing an intelligent algorithm and combining a thermodynamic model, a dynamic model and specificity evaluation, an optimal primer and probe combination is automatically generated, and the stability and reliability of design are ensured.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The present invention provides a use of a Cas protein, and a method and a kit for detecting target nucleic acid molecules. The method for detecting target nucleic acid molecules comprises adding a guide RNA, a Cas12a, and a nucleic acid probe into a reaction system containing target nucleic acid molecules to be detected, and detecting it after the reaction is completed.

The present disclosure relates, in some aspects, to methods and compositions for analyzing a biological sample. In some aspects, the methods comprise use of a probe set comprising a first and second polynucleotide which hybridize to a target nucleic acid in the biological sample and form a circularized probe. In some aspects, the methods and compositions provided herein improve the detection of nucleic acids in a biological sample.

The invention relates to a kit for detecting the sensitivity of kidney cancer to a targeted drug, which comprises an RNA (Ribonucleic Acid) nucleic acid probe, and the sequence of the RNA nucleic acid probe is shown as SEQ ID NO: 4. The invention also provides a construction method of the specific annular RNA probe. The construction method comprises the following steps: S1, designing and constructing a recombinant plasmid containing a target gene; s2, constructing a tricRNA (Ribonucleic Acid) vector containing a reverse complementary sequence crossing a backspace junction site and a Broccoli sequence, wherein the reverse complementary sequence crossing the backspace junction site and the Broccoli sequence are subjected to reverse complementary sequence crossing the backspace junction site; s3, carrying out vector transfection on the HEK293T cell to express a tricRNA probe, and extracting the tricRNA probe; and S4, constructing a tricAGAP1 Probe-Broccoli probe. The method comprises the following steps of: constructing a tricAGAP1 probe; the invention provides application of a probe in preparation of a cell imaging probe and in preparation of a kit for detecting sensitivity of kidney cancer to a targeted drug. The invention not only provides a new method and technology for guiding the medication of sunitinib for patients with advanced kidney cancer, but also provides a technical example for the development of other circRNA detection technologies.

The invention discloses a dual-recognition-framework nucleic acid probe, a preparation method and application, the dual-recognition-framework nucleic acid probe comprises a DNA tetrahedron, and a dual-recognition module and a fuel amplification module which are connected to the vertex of the DNA tetrahedron through complementary pairing of bases, ATP recognition protection and dual-target response are introduced, and the dual-recognition-framework nucleic acid probe is obtained through sequential opening of internal and external locks. The occurrence of false positive conditions can be reduced, and the specificity is effectively improved; signal amplification is realized by virtue of abnormal APE1 expression of tumor cells, so that a fluorescence signal for miRNA detection is increased, and the sensitivity and the imaging signal-to-noise ratio are effectively improved; the preparation is easy; the use is convenient; complicated equipment is not needed. The method can be applied to a target nucleic acid detection reagent or an imaging or positioning reagent of cells, tissues or organs containing target nucleic acid.

The embodiment of the invention provides a connecting unit for chemical modification of a nucleic acid end group, a preparation method of the connecting unit and a nucleic acid probe. The connecting unit for chemical modification of the nucleic acid end group has a triethylene glycol structure, and the connecting unit has the following structure. Therefore, the connecting unit with the triethylene glycol structure is relatively simple in synthesis process, relatively high in yield, efficient in synthesis, good in nucleic acid end group chemical modification effect, applicable to connection of various types of probe molecules and nucleic acid molecules, capable of simply and efficiently preparing nucleic acid probes and wide in application range.

The present invention belongs to the technical field of immunochromatography. Provided are a streptavidin multimer, and a preparation method therefor and the use thereof. The prepared streptavidin multimer has a strong affinity for a biotin / fluorescein nucleic acid probe. A nucleic acid test strip prepared with the prepared streptavidin multimer can realize rapid and high-sensitivity detection of a sample to be detected. Results can be obtained in 5 minutes, the false positive rate can be reduced, and the cost of the nucleic acid probe is reduced, which is convenient for non-professional technicians to easily and accurately judge the results.

The invention discloses detection test paper and a detection kit for detecting telomerase activity and application, the detection test paper comprises a nitrocellulose membrane, the surface of the nitrocellulose membrane is provided with a test area, and a first detection line and a second detection line are printed in the test area; wherein the first detection line and the second detection line are respectively formed by printing a first capture probe sequence and a second capture probe sequence of streptavidin-biotinylation, the first capture probe is used for capturing a DNA neck ring structure extending after telomerase reaction, and the second capture probe is used for capturing a DNA sequence with a fluorophore replaced after telomerase reaction. Meanwhile, the invention discloses a detection kit, which contains a colloidal gold nucleic acid probe solution, can realize rapid dual visual detection of telomerase activity through colloidal gold color development and fluorescence detection, and has the advantages of sensitive and rapid response to telomerase extension products and high sensitivity. The problem of false positive rate in telomerase activity detection can be greatly reduced.

The invention provides a primer / probe composition for capillary electrophoresis analysis, a detection method and application. The primer / probe composition comprises a forward primer, a reverse primer and a nucleic acid probe, the 5'end of the nucleic acid probe carries a fluorescent label, and both the forward primer and the reverse primer do not carry the fluorescent label. Different targets are identified by detecting the nucleic acid probe, so that the length of an amplification product is not limited, only the nucleic acid probe carries a fluorescence signal without generating a non-specific fluorescence signal, the generation of a supersaturated fluorescence signal can be effectively prevented by using the nucleic acid probe with a specific concentration, the fluorescence signals among different targets do not interfere with each other, and the detection sensitivity is high. And multicolor fluorescence can be used at the same time.

The invention provides a nucleic acid probe, a primer group, a kit, a detection method and application, and relates to the technical field of biology. The nucleotide sequence of the nucleic acid probe is as shown in SEQ ID NO. 1; wherein the 3'end of the nucleic acid probe is modified with MGB, and locked nucleic acid is modified at a base position corresponding to the difference between the swine fever wild strain and the swine fever vaccine strain in the nucleotide sequence. According to the nucleic acid probe, a specific sequence is utilized to precisely target a swine fever wild strain, and locked nucleic acid modification is introduced at a differential site, so that the recognition capability on single-base mismatch is remarkably enhanced, non-specific binding to a vaccine strain is thoroughly blocked, and false positive interference is eliminated; and 3 '-terminal MGB modification is matched to improve the binding stability and sensitivity, so that the efficient and accurate identification of the swine fever wild strain under the immune background is ensured.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The present invention generally relates to systems and methods for imaging or determining nucleic acids in cells or other samples. In some cases, the transcriptome of a cell may be determined. Certain embodiments are generally directed to determining nucleic acids and other targets in a sample at relatively high resolutions. For instance, nucleic acid probes may be applied to sample, and binding of the nucleic acid probes to a target may be amplified using primary and secondary amplifier nucleic acids. In some cases, there is a maximum number of amplifier nucleic acids that can be bound to a target, e.g., the binding is saturatable, and cannot grow indefinitely, even in the presence of abundant reagents. This may be advantageous, for example, for controlling the brightness of each binding event, controlling the size of the amplified regions (e.g., during imaging), and / or for limiting the degree of amplification noise (i.e. the final variation in amplified signal from molecule to molecule), etc. In addition, in some embodiments, the primary and / or secondary amplifier nucleic acids may be formed from only 3 of the 4 naturally-occurring nucleotides, which may result in less secondary structure, faster binding rates, etc. These properties can in some cases facilitate the rapid design of multiple orthogonal amplification sequences, allowing the extension of such an approach to many distinct molecular targets.

Methods and systems for detecting the presence of a target nucleic acid sequence in one or more samples of a plurality of samples are described. The methods may comprise the use of linear barcoded nuc

The invention discloses a multi-nucleic acid probe rapid modification and multi-target detection device based on electric field regulation and control. An instrument main body in the device is integrated with an automatic liquid path system and a micro electrochemical workstation module, and is used for realizing programmable reagent and sample conveying and multi-channel electrochemical measurement. The detachable sensing chip is formed by bonding a microelectrode array chip and a micro-fluidic chip, and is switched through a PCB (Printed Circuit Board) to realize plug and play. According to the present invention, the positive suction-negative discharge strategy of the electric field is utilized, the preferred positive potential is applied to the target electrode to efficiently attract the probe, and the preferred negative potential is applied to the non-target electrode to inhibit the non-specific adsorption so as to achieve the spatial selective rapid fixation and the accurate density regulation and control of the multi-nucleic acid probe in the extremely short time; the device can be used for quantitatively detecting various body fluid disease markers in a plurality of urine samples at the same time in single operation, and an efficient, accurate and portable multi-marker comprehensive analysis solution is provided for early screening and instant diagnosis of tumors.

The invention relates to a streptavidin polymer as well as a preparation method and application thereof, and belongs to the technical field of immunochromatography. The prepared streptavidin polymer has strong affinity with a biotin / fluorescein nucleic acid probe, nucleic acid test paper prepared from the prepared streptavidin polymer can realize rapid and high-sensitivity detection on a detected sample, a result can be obtained within 5 minutes, the false positive rate can be reduced, the cost of the nucleic acid probe is reduced, and the method is suitable for popularization and application. And non-professional technicians can conveniently, easily and accurately judge the result.

The invention belongs to the technical field of biochemical detection, and particularly relates to a micro-fluidic chip PSMA prostate cancer circulating tumor cell immunoenrichment detection kit and a preparation method thereof. According to the kit, an EpCAM antibody, a Vimentin antibody and a double-site PSMA capture combination are fixed in a chip channel through a streptavidin-biotin system, double enrichment of epithelial and mesenchymal CTCs is achieved, PSMA targets are recognized through combination of an aptamer and the antibody, and the detection specificity and sensitivity are improved. Competitive oligopeptides are further introduced to block non-specific binding sites on the surfaces of leukocytes and reduce false positive. By combining a multiple fluorescent chromosome system and a double-antibody nucleic acid probe signal amplification strategy, accurate recognition and quantification of CTC and PSMA positive cells are realized. The scheme is suitable for early screening, curative effect evaluation and relapse monitoring of prostate cancer, and has a good clinical application prospect.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The utility model discloses an electrophoresis gene chip, which relates to the technical field of gene chips and comprises a substrate and a cover plate, nucleic acid probes are fixed at a plurality of positions on the upper surface of the substrate, a conductive coating is arranged on the lower surface of the substrate, and the substrate is used as a first electrode; the cover plate is positioned above the substrate; a plurality of strip electrodes are regularly arranged on the upper surface of the cover plate, and each strip electrode serves as a second electrode; the first electrode and the plurality of second electrodes are connected with an external power supply controller through leads; the first electrode and the plurality of second electrodes are used for alternately carrying positive and negative electricity under the control of the power supply controller, so that magnetic particles in the to-be-detected solution between the substrate and the cover plate move in a three-dimensional space between the substrate and the cover plate and are sequentially enriched in a corresponding area of each second electrode on the substrate; the to-be-detected solution contains sample nucleic acid combined with the magnetic particles. By adopting the electrophoresis gene chip, sample nucleic acid in a solution to be detected can be driven to move in a three-dimensional direction.

The invention discloses a method for ultrasensitive electrochemical detection of port carp spring viremia virus based on gold nanoparticle signal amplification, comprising the following steps: S1. preparing MBs-probe 1; S2. preparing AuNPs-probe 2; S3. preparing AuNPs-probe 4; S4. gold-plating the surface of the screen-printed electrode and covalently modifying the nucleic acid probe on the surface, then adsorbing hexaamine ruthenium, performing electrochemical determination, and obtaining the concentration of SVCV. The present invention establishes an ultrasensitive portable electrochemical detection method for SVCV based on the principle of gold nanoparticle signal amplification adsorbed by hexaamine ruthenium by combining high-affinity probes, which can amplify the signal geometrically, greatly improve the sensitivity, and the detection limit of SVCV is 0.0646nM, the recovery rate is 95%-102%, the specificity is high, the stability and reproducibility are good, the operation is simpler, the time required for detection is shortened, and the detection efficiency is improved.

A method of detecting the presence of Neisseria gonorrhoeae in a sample. The method involves detecting a first target sequence taken from the NGO1642 gene and / or a second target sequence taken from the NGO1012 gene. The method may involve a step of amplifying the target sequence and may involve hybridising the target sequence to a nucleic acid probe and identifying hybridisation. The method may involve simultaneous detection of other target sequences, e.g., from other pathogens.

The invention relates to the technical field of molecular diagnosis and graphic processing, in particular to an electronic microarray chip dot matrix negative and positive judgment method based on an ISFET sensor. A chip hole site corresponds to an ISFET sensor, and after a hole bottom specific nucleic acid probe and a target sequence are hybridized and extended, the voltage of the sensor is changed. The method specifically comprises the following steps: data preprocessing: filtering median values of multi-frame electric signals of a hole site and taking a mean value; the dot matrix identification comprises the steps of threshold demarcation, Canny edge detection, circular Hough transform, optimal circle screening by Kdtree, array numbering and abnormal deletion processing to position a dot matrix; and calculating a result, extracting a signal difference value of the D1 stage and the D4 stage, evaluating numerical values of the positive quality control array and the to-be-detected sample array through a numerical value of the negative quality control array, and judging a final calculation result. According to the method, response changes are rapidly analyzed through image recognition, false detection and missing detection caused by numerical value differences in different arrays are reduced through multiple times of detection, and the detection accuracy is improved by selecting the most possible area through tickets.

The present disclosure is generally directed to anchoring bridge nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid, contacting the anchored bridge probes with primary probes that hybridize with the bridge probes and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

The invention discloses a preparation method of a space chip, the space chip and application of the space chip, the preparation method comprises the following steps: hybridizing: connecting a single-stranded nucleic acid molecule to the surface of a solid-phase substrate, the single-stranded nucleic acid molecule comprises a first fixed sequence, a space bar code sequence, a sequencing primer binding sequence and a second fixed sequence, the surface of the solid-phase substrate of the chip substrate is connected with a first probe, and the first probe comprises a first fixed sequence complementary sequence and a second fixed sequence complementary sequence; carrying out ring closing to enable the single-stranded nucleic acid molecules to form annular nucleic acid molecules; carrying out rolling circle amplification; sequencing to obtain sequence information of the space bar code sequence; synthesizing single-stranded nucleic acid molecules by taking the rolling circle amplification product as a template to obtain a single-stranded nucleic acid molecule library; fixing and clustering: fixing the single-stranded nucleic acid molecule library on the surface of a solid-phase substrate to form a single-stranded nucleic acid molecule cluster. According to the invention, the chip with the nucleic acid probe carrying spatial position coordinate information can be prepared.

The present disclosure relates to finely modulated ultra-specific nucleic acid hybridization probes. The present invention provides compositions and methods for highly specific nucleic acid probes and primers. The probe system includes a complementary strand and a protective strand to partially form a double-stranded probe. The reaction standard free energy ([delta] G DEG rxn = [delta] G DEG t-TC-[delta] G DEG nh-PC + ([delta] G DEG v-TC-[delta] G DEG h-PC)) of hybridization between the probe and the target nucleic acid determined by formula 1 is about-4 kcal / mol to + 4 kcal / mol. Alternatively, the reaction standard free energy of hybridization between the probe and the target nucleic acid determined by Formula 1 is within 5 kcal / mol of the standard free energy determined by Formula 2 (-R [tau] ln (([P] 0-[C] 0) / [C] 0)), the term [P] 0 of Formula 2 being equal to the concentration of the protective strand, and the term [C] 0 of Formula 2 being equal to the concentration of the complementary strand. In addition, the present invention provides a method for the immediate fine modulation of a reaction using the probe of the present invention.