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40 results about "Hybridization probe" patented technology
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In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100–10000 bases long) which can be radioactively or fluorescently labeled. It can then be used in DNA or RNA samples to detect the presence of nucleotide substances (the RNA target) that are complementary to the sequence in the probe. The probe thereby hybridizes to single-stranded nucleic acid (DNA or RNA) whose base sequence allows probe–target base pairing due to complementarity between the probe and target. The labeled probe is first denatured (by heating or under alkaline conditions such as exposure to sodium hydroxide) into single stranded DNA (ssDNA) and then hybridized to the target ssDNA (Southern blotting) or RNA (northern blotting) immobilized on a membrane or in situ. To detect hybridization of the probe to its target sequence, the probe is tagged (or "labeled") with a molecular marker of either radioactive or (more recently) fluorescent molecules; commonly used markers are ³²P (a radioactive isotope of phosphorus incorporated into the phosphodiester bond in the probe DNA) or digoxigenin, which is a non-radioactive, antibody-based marker. DNA sequences or RNA transcripts that have moderate to high sequence similarity to the probe are then detected by visualizing the hybridized probe via autoradiography or other imaging techniques. Normally, either X-ray pictures are taken of the filter, or the filter is placed under UV light. Detection of sequences with moderate or high similarity depends on how stringent the hybridization conditions were applied—high stringency, such as high hybridization temperature and low salt in hybridization buffers, permits only hybridization between nucleic acid sequences that are highly similar, whereas low stringency, such as lower temperature and high salt, allows hybridization when the sequences are less similar. Hybridization probes used in DNA microarrays refer to DNA covalently attached to an inert surface, such as coated glass slides or gene chips, to which a mobile cDNA target is hybridized.
Methods and compositions for analyte detection using diluted readout signals
In some aspects, the present disclosure generally relates to methods and compositions for detecting a plurality of molecules of one or more analytes in a sample. In some aspects, the present disclosure relates to methods for determining the locations and identities of analytes in a biological sample using detectable probes. In some aspects, the present disclosure relates to methods for reducing the crowding of signals that are used for determining the locations of the analytes (e.g., spot-calling signals). The methods herein have particular applicability in the detection of spot-calling signals and barcode sequences in sequencing-by-hybridization (SBH) methods, including those relying on combinatorial labeling schemes and decoding of the barcodes by sequential cycles of spot-calling and / or decoding using hybridization probes.
Owner:10X GENOMICS INC
Suppression of non-specific signals by exonucleases in fish experiment
Disclosed herein is a composition for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a kit for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples. Also disclosed herein, is a method for nuclease treatment of hybridized probes in experiments to profile and analyze biological samples.
Owner:CALIFORNIA INST OF TECH
Liquid phase hybridization probe pool, kit and joint detection method for joint detection of multiple pathogens and drug-resistant genes
ActiveCN120843744AMicrobiological testing/measurementMicroorganism based processesHybridization probeDrug resistance
The invention discloses a liquid-phase hybridization probe pool for joint detection of multiple pathogens and drug-resistant genes. The liquid-phase hybridization probe pool is designed according to target areas of target pathogens and drug-resistant genes; the probe is a probe with unequal length of 30 to 100 nt; comprising a target specific sequence complementarily paired with a target region and a conjugate sequence complementarily paired between adjacent probes; the 5'terminal and the 3 'terminal of the probe are respectively modified with a conjugate sequence A and a conjugate sequence B, so that the adjacent probes can be specifically combined through terminal pairing; the conjugate sequence A is as shown in SEQ ID No. 601, and the conjugate sequence B is as shown in SEQ ID No. 602. According to the method, the constructed library and the specific probe pool are hybridized, so that the capture time is shortened to 30 minutes, and the method is obviously superior to a traditional method. Through optimization, the operation time and complexity are reduced, personal errors are reduced, meanwhile, high sensitivity and specificity are kept, target nucleic acid in a complex sample can be efficiently captured, a high-quality template is provided for subsequent sequencing, and combined detection of multiple respiratory pathogens and drug-resistant genes is supported.
Owner:BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
Methods and compositions for enrichment and sequencing of expansion-specific RNA transcripts
PCT designated stage expiredWO2024263649A1Microbiological testing/measurementDiseaseHybridization probe
Methods of isolating and sequencing aberrant expansion-specific RNA sequences using antisense oligonucleotides (ASO) specific for repeat-containing RNAs have been established. The methods isolate repeat-containing RNAs from RNA using hybridization probes specific for repeat-containing sequences. Typically, RNA capture is performed by preferential hybridization of repeat-containing RNAs to a multiplicity of immobilized probes. The methods isolate expansion-specific RNAs and determine the sequences of aberrant splice variants associated with diseases and disorders. In other forms, the methods identify small molecules that selectively target the pathological splice variant transcripts. In some forms, the methods provide expansion-specific ASOs that specifically target and eliminate the pathological splice variant transcripts in vivo. Compositions of ASOs that specifically target and eliminate the pathological splice variant transcripts are also provided.
Owner:YALE UNIVERSITY
Nucleic acid assays using click chemistry bioconjugation
Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.
Owner:10X GENOMICS INC
Methods, compositions and systems for protein detection
PCT designated stageWO2025212672A1Microbiological testing/measurementAssay labelsMultiplexProtein detection
The present disclosure relates to proteomics, including the detection of immune cell target proteins as examples that the disclosed methods can be used for protein detection. Methods, compositions, and systems are described for identifying the presence of proteins, including detecting immune cell proteins from a sample using a combination of multiplex immuno-PCR with antibodies conjugated to oligonucleotides comprising an antibody-specific barcode flanked by PCR priming sites; detection of barcoded amplicons obtained from immuno-PCR with encoded padlock probes comprising codes specific for the barcodes / target protein; rolling circle amplification (RCA) of the circularized padlock probes; and determination of the sequence of the codes amplified by RCA by next generation sequencing or using fluorescently labeled hybridization probes.
Owner:PLENO INC
Specific cell population analysis and screening method based on RCA-FISH and application thereof
PendingCN119639872Aavoid restrictionseasy to transportMicrobiological testing/measurementIndividual particle analysisFluoProbesHybridization probe
The invention discloses a specific cell population analysis and screening method based on RCA-FISH and application of the specific cell population analysis and screening method. A target sequence region which is high in hybridization efficiency, high in specificity and free of an RNA advanced structure is screened out from an RNA sequence of a cell or tissue to be detected to design a primary hybridization probe, and the primary hybridization probe is composed of a lock-type probe and an RCA initiation probe; the method comprises the following steps: pre-treating a primary probe, hybridizing, carrying out a probe cyclization connection reaction, amplifying a signal by rolling circle amplification, and labeling cells by a fluorescent probe, so as to be used for flow cytometry analysis / sorting. The invention has the characteristics of simple probe preparation, high detection efficiency, strong specificity, strong signal amplification and the like.
Owner:HUAZHONG AGRI UNIV
Molecule beacon-based hybridization sensor for the detection of a single nucleotide variation in folded nucleic acids
PendingUS20250129409A1Microbiological testing/measurementFluorescence/phosphorescenceLow affinityHybridization probe
Hybridization probes have been used in the detection of specific nucleic acids for the last 50 years. Despite the extensive efforts and the great significance, the challenges of the commonly used probes include (1) low selectivity in detecting single nucleotide variations (SNV) at low (e.g., room or 37° C.) temperatures; (2) low affinity in binding folded nucleic acids, and (3) the cost of fluorescent probes. To address all three issues, a multicomponent hybridization probe, called OWL2 sensor, is introduced. OWL2 sensor uses two analyte binding arms to tightly bind and unwind folded nucleic acid analytes, and two sequence-specific strands that bind both the analyte and a universal molecular beacon (UMB) probe to form fluorescent ‘OWL’ structure. OWL2 sensor can differentiate single base variations in folded analytes in a temperature range of 5-38° C. The design is cost-efficient since the same optimized fluorescently labeled UMB probe can be used for the detection of any analyte sequence.
Owner:UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION INC
Fine-adjusted super-specific nucleic acid hybridization probe
PendingCN120945018ASugar derivativesMicrobiological testing/measurementHybridization probeFree energies
The present disclosure relates to finely modulated ultra-specific nucleic acid hybridization probes. The present invention provides compositions and methods for highly specific nucleic acid probes and primers. The probe system includes a complementary strand and a protective strand to partially form a double-stranded probe. The reaction standard free energy ([delta] G DEG rxn = [delta] G DEG t-TC-[delta] G DEG nh-PC + ([delta] G DEG v-TC-[delta] G DEG h-PC)) of hybridization between the probe and the target nucleic acid determined by formula 1 is about-4 kcal / mol to + 4 kcal / mol. Alternatively, the reaction standard free energy of hybridization between the probe and the target nucleic acid determined by Formula 1 is within 5 kcal / mol of the standard free energy determined by Formula 2 (-R [tau] ln (([P] 0-[C] 0) / [C] 0)), the term [P] 0 of Formula 2 being equal to the concentration of the protective strand, and the term [C] 0 of Formula 2 being equal to the concentration of the complementary strand. In addition, the present invention provides a method for the immediate fine modulation of a reaction using the probe of the present invention.
Owner:WILLIAM MARCH RICE UNIVERSITY
Phosphorylation probe capture amplification method
The invention provides a phosphorylated probe capture amplification method. The method comprises the following steps: hybridizing a phosphorylated probe to genome DNA to form a hybridization probe, extending and connecting the hybridization probe to form a closed loop by using a bridging primer to form a closed loop PCR product, and carrying out PCR amplification on the obtained PCR product to obtain an amplified product which is an enriched target area. According to the application, phosphorylation is carried out by taking the probe as a skeleton, a sequence on an Illumina Tru Seq joint and a bridged complementary sequence are added, by adopting a mode of increasing primer bridging, products after probe amplification are connected end to end to form cyclization, and by adopting a mode of increasing EXO1 / EXO3 enzyme digestion, the formation of a dimer is reduced, so that the detection sensitivity is improved, and the detection sensitivity is improved. According to the invention, small-volume hybridization and small-volume amplification modes are adopted, so that the molecular collision probability is increased. The method has ultrahigh specificity and accuracy, each target site is independently amplified, the PCR preference is avoided, the coverage uniformity is superior to that of multiple PCR and hybrid capture, the operation is simple and convenient, the period is short, and the experimental process only needs several hours.
Owner:SHANGHAI HEYIN BIOTECHNOLOGY CO LTD
Chip capable of firstly capturing and then sequencing and sequencing method thereof
PendingCN119161968ABioreactor/fermenter combinationsBiological substance pretreatmentsEnzyme digestionHybridization probe
The invention belongs to the technical field of molecular biology, and particularly relates to a chip capable of firstly capturing and then sequencing and a sequencing method thereof. The sequencing chip comprises an upper substrate and a lower substrate, wherein the inner surfaces of the two substrates are connected with a P5 sequence and a P7'sequence with a hybridization probe; according to the chip disclosed by the invention, through library building cyclization, target sequence capture, target sequence enrichment through rolling circle amplification, target library sequence release through enzyme digestion, hybridization clustering and sequencing, the target variety is greatly increased, the specificity is improved, and meanwhile, the sequencing cost is reduced.
Owner:HANGZHOU YISU MICRO CONTROL GENE TECH CO LTD
Fluorescence in-situ hybridization probe group for rapidly distinguishing hexaploid oat chromosomes and application of fluorescence in-situ hybridization probe group
PendingCN120796548AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyIn situ hybridisation
The invention discloses a fluorescence in-situ hybridization probe group for rapidly distinguishing hexaploid oat chromosomes and application thereof, and belongs to the technical field of molecular biology. The fluorescent in-situ hybridization probe group provided by the invention comprises a probe oligo-356 and a probe oligo-898, wherein the nucleotide sequence of the probe oligo-356 is as shown in SEQ ID NO. 1; the nucleotide sequence of the probe oligo-356 is as shown in SEQ ID NO. 2; the provided fluorescence in-situ hybridization probe group can quickly and accurately identify different chromosomes of hexaploid oat through a fluorescence in-situ hybridization method, and has important scientific and practical values for hexaploid oat chromosome structure variation identification, oat genetic relationship identification, oat genetic map construction and oat variety improvement.
Owner:SICHUAN AGRI UNIV
A bone marrow smear FISH test kit and its application
PendingCN122357694AHybridization probeSignal interpretation
This application relates to the field of molecular pathology diagnostic technology, specifically disclosing a bone marrow smear FISH detection kit and its application. The kit includes a rapid hybridization buffer, a stabilization pretreatment solution, room temperature hybridization probes, probe dilution buffer, low-salt washing buffer, high-salt washing buffer, counterstaining solution, positive control samples, and negative control samples. The room temperature hybridization probes include broken probe pairs targeting the BCR-ABL, PML-RARA fusion genes, and IgH gene rearrangements. The detection method of this kit includes sample pretreatment, probe denaturation, room temperature hybridization, washing and counterstaining, and signal interpretation. Through the synergistic effect of the rapid hybridization buffer and the short-chain low-Tm value probes, rapid hybridization at room temperature is achieved, eliminating the need for a dedicated isothermal hybridization instrument. The stabilization pretreatment solution can complete cell permeation and RNA removal in one step, resulting in a high degree of operational standardization.
Owner:SUZHOU YUANDE YOUQIN MEDICAL LAB CO LTD
A marker composition, product and use thereof for lung cancer detection or diagnosis
ActiveCN121109595BMicrobiological testing/measurementDNA/RNA fragmentationHybridization probeSpecific detection
This invention discloses a biomarker composition, product, and application for lung cancer detection or diagnosis, relating to the field of lung cancer diagnostic technology. The biomarker composition provided by this invention has the advantages of high specificity and high sensitivity; 100% specificity means a zero false positive rate, avoiding unnecessary anxiety, unnecessary radiation exposure, and further invasive examinations. Based on the lung cancer detection or diagnostic biomarker composition provided by this invention, lung cancer diagnostic products, such as reagents, kits, chips, hybridization probes, or sequencing libraries, can be further developed. This will help achieve rapid, highly sensitive, and highly specific detection or diagnosis of lung cancer.
Owner:JIAXING YUNYING MEDICAL INSPECTION CO LTD
Peripheral blood neutrophil molecular typing kit and its application in early diagnosis of lung cancer
This invention relates to a molecular typing kit for peripheral blood neutrophils and its application in the early diagnosis of lung cancer, belonging to the field of oncology technology. The kit comprises a target sequence hybridization probe and a fluorescent reporter probe; the nucleotide sequence of the target sequence hybridization probe is shown in SEQ ID NO: 11-25; the nucleotide sequence of the fluorescent reporter probe is shown in SEQ ID NO: 26. This invention designs the target sequence hybridization probe so that one part hybridizes with the target gene and the other part hybridizes with the fluorescent reporter probe. The fluorescence value of the fluorescent reporter probe can identify or differentiate target cells. Using this kit, neutrophils can be identified or classified into neutrophil subsets that are positive for CXCL8, LYZ, IL1R2, S100A12, and IFI16 genes. These gene-positive neutrophil subsets can be used to diagnose early-stage cancer.
Owner:SUN YAT SEN UNIV
Systems and methods for designing accurate fluorescence in-situ hybridization probe detection on microscopic blood cell images using machine learning
In some embodiments, a non-transitory processor-readable medium stores code representing instructions to be executed by a processor. The code includes code to cause the processor to receive a plurality of sets of images associated with a sample treated with fluorescence in situ hybridization (FISH) probes. Each image from that set of images is associated with a different focal length using a fluorescence microscope. Each FISH probe can selectively bind to a unique location on chromosomal DNA in the sample. The code further causes the processor to identify cell nuclei in the images. The code further causes the processor to apply a convolutional neural network (CNN) to each set of images. The CNN is configured to identify a probe indication from a plurality of probe indications for that set of images. The code further causes the processor to identify the sample as containing circulating tumor cells.
Owner:LUNGLIFE AI INC
Methods and systems for characterizing analytes from individual cells or cell populations
The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.
Owner:10X GENOMICS INC
Construction method and application of sequencing library without human nucleic acid
ActiveCN119753090AMicrobiological testing/measurementLibrary creationHybridization probeSingle strand
The invention discloses a sequencing library for removing human nucleic acid and a construction method and application thereof, and the construction method comprises the following steps: fragmenting nucleic acid, adding a sequencing joint, constructing a DNA or RNA library, or co-constructing a library by RNA and DNA, taking the library added with the sequencing joint as a template, carrying out denaturation annealing on the library and a specific human hybridization probe, digesting by using DSN enzyme, and carrying out sequencing on the human nucleic acid. In the renaturation process, the human nucleic acid with higher content is firstly annealed with the human hybridization probe to form double strands, the double strands are degraded by DSN enzyme, the pathogenic microorganisms with low content and slow renaturation still keep a single strand state and cannot be digested by the DSN enzyme, the human nucleic acid is effectively removed, and the pathogenic microorganism nucleic acid is further enriched through subsequent PCR (Polymerase Chain Reaction). According to the method, the proportion of human nucleic acid in the library can be effectively reduced, and nucleic acid information of pathogenic microorganisms can be reserved to the maximum extent.
Owner:JIANGSU BIOPERFECTUS TECH CO LTD
Hybrid probe mixed solution for radiation biological dose detection and preparation method and product thereof
PendingCN122081478AMicrobiological testing/measurementDNA/RNA fragmentationHybridization probeHigh radiation
The invention relates to a hybridization probe mixed solution for radiation biological dose detection as well as a preparation method and a product thereof, and belongs to the field of molecular cell genetics. The technical problem to be solved is to provide a hybridization probe mixed solution with significantly improved chromosome distortion recognition accuracy and sensitivity, higher detection efficiency and better repeatability. According to the key point of the technical scheme, the hybridization probe mixed solution is composed of a probe and a hybridization buffer solution, the volume ratio of the probe to the hybridization buffer solution is 1: (50-1000); the hybridization buffer solution is a 40%-70% formamide solution containing 10 to 20 mM of Tris-HCl or PBS (Phosphate Buffer Solution). When the hybridization probe mixed solution is used for radiation biological dose detection, the distortion identification accuracy and sensitivity are high, the radiation dose estimation precision is high, and the operation is convenient.
Owner:NAT INST FOR RADIOLOGICAL PROTECTION & NUCLEAR SAFETY CHINESE CENT FOR DISEASE CONTROL & PREVENTION
Systems, methods, and compositions for digital drop-off assays
The present disclosure provides methods, systems, and compositions for the multiplexed detection and quantification of multiple analytes from a sample. Analytes may be nucleic acid analytes. Detection of analytes may comprise contacting one or more samples with primers and / or hybridization probes to generate cumulative signal measurements. The methods may comprise digital PCR or may comprise partitioning a sample into multiple partitions.
Owner:CHROMACODE INC
Design and application method of plant generic genome exon chip
PendingCN120099156AMicrobiological testing/measurementHybridisationGenomic sequencingHybridization probe
The invention provides a design and application method of a plant generic genome exon chip, and aims to reduce the genome sequencing cost of wheat and other complex genome crops and improve the sequencing efficiency. The technology comprises the following steps: 1, designing an efficient liquid phase capture probe through an iterative algorithm, and ensuring that the probe can cover more than 99% of exon regions of a target genome; 2, calculating an original probe template for grouping and synthesizing the exon chips by adopting an algorithm; 3, preparing a liquid phase capture probe by using an ultramicro amplification method; 4, performing experimental hybridization through a grouping gradient hybridization method to ensure efficient capture of a target area; and 5, performing high-throughput sequencing on the captured sample by using a next-generation sequencing platform to obtain high-quality exon region data. The technology can be applied to the fields of genetic research, breeding, whole genome selection and the like of wheat and other complex genome crops. By optimizing probe design, hybridization probe preparation and capture methods, the sequencing cost of plants with complex genomes is remarkably reduced, the coverage degree and data accuracy of important regions of the genomes are improved, and the method is suitable for large-scale plant genomics research, germplasm resource evaluation and identification and large-scale mutant library digitization.
Owner:CHENGDU TIANCHENG SMART AGRICULTURAL TECHNOLOGY CO LTD
Methods, systems and compositions for detection of multiple analytes
The present disclosure provides methods, systems, and compositions for the multiplexed detection and quantification of multiple analytes from a sample. Analytes may be nucleic acid analytes. Detection of analytes may comprise contacting one or more samples with primers and / or hybridization probes to generate cumulative signal measurements. The methods may comprise digital PCR or may comprise partitioning a sample into multiple partitions.
Owner:CHROMACODE INC
Preparation method of fluorescence in situ hybridization kit based on hybridization chain reaction (HCR)
PendingCN120796445AMicrobiological testing/measurementDNA/RNA fragmentationHybridization probeParaformaldehyde
The invention belongs to the technical field of molecular biology, and discloses a preparation method of a fluorescence in situ hybridization kit based on a hybridization chain reaction (HCR), the core components of the kit comprise a hybridization probe, an amplification probe, a washing liquid, a confining liquid, protease K, HRP-mouse anti-digoxin, and a TSA developing liquid 520 nm. According to the FISH detection system combined with the HCR signal amplification technology, when a hybridization probe and an amplification probe are simultaneously combined to a position adjacent to a target sequence, a small section of sequence at the top end can trigger a branched HCR reaction to form a macromolecular nucleic acid aggregate. Signal amplification of HCR is achieved through cascade hybridization reaction, detection sensitivity and specificity can be remarkably improved, and meanwhile the problem of background noise caused by a traditional enzymatic method is avoided. The kit disclosed by the invention is wide in sample application range, and can be used for positioning analysis of target RNA of samples such as paraformaldehyde or formalin-fixed animal and plant tissues, paraffin or frozen sections, cells and the like, and high-sensitivity detection of the single cell level of non-coding RNA.
Owner:HANGZHOU BORRICK BIOTECHNOLOGY CO LTD
Composition and methods for hybridization
The present invention relates to a composition for use in hybridization, the composition comprising: (a) at least one hybridization probe (“component (a)”); (b) at least one polar protic or polar aprotic solvent (“component (b)”) having a cyclic molecule structure; and (c) at least one carboxylic acid amide and / or salts thereof (“component (c)”), in an amount of more than 10 vol %, based on the composition. The invention furthermore relates to a use of this composition, and to methods for detecting nucleic acids and / or chromosomal aberrations in a biological sample by way of hybridization, using the composition.
Owner:42 LIFE SCI GMBH & CO KG
Methods and systems for digital multiplex analysis
The present disclosure provides methods, systems, and compositions for the multiplexed detection and quantification of multiple analytes from a sample. Analytes may be nucleic acid analytes. Detection of analytes may comprise contacting one or more sample subsets with hybridization probes to generate cumulative signal measurements. The methods may comprise digital PCR or may comprise partitioning a sample into multiple partitions.
Owner:CHROMACODE INC
Method to predict the pattern of locomotion in horses
PendingUS20250122573A1Microbiological testing/measurementBiological material analysisHybridization probeGenetics
The present invention provides methods for predicting the pattern of locomotion in a horse including the ability of a horse to use different gaits and the ability to trot at a fast speed. The methods comprise determining in a sample of DNA obtained from a horse the presence or absence of at least one genetic marker, wherein said at least one genetic marker is located on horse chromosome 23, said marker being associated with the ability to use different gaits. The invention further provides primers that amplify markers being associated with the ability to use different gaits and hybridization probes to detect markers being associated with the ability to use different gaits and the ability to trot at a fast speed.
Owner:CAPILET GENETICS
Hybridization probes containing fluorinated carbon chains and related methods
In one aspect, disclosed herein are new hybridization probes that contain fluorinated carbon tags (F), methods of making these hybridization probes, and methods for using these hybridization probes for affinity capture of the probes both in purification during production and in the enrichment process using fluorous substrates. In certain embodiments, the hybridization probe comprises a) a polynucleotide having a 3′ end and a 5′ end and comprising about 20 to about 200 nucleotide units and b) one or more fluorinated affinity tags, wherein each affinity tag comprises one or more polyfluorinated carbon chains each comprising 3-30 carbon atoms; wherein the polynucleotide comprises a sequence complementary or substantially complementary to a target sequence within a target nucleic acid.
Owner:UNIV OF UTAH RES FOUND +1
Nucleic acid assays using click chemistry bioconjugation
Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.
Owner:10X GENOMICS INC
Methods and compositions for genomic target enrichment and selective DNA sequencing
ActiveUS12492423B2Microbiological testing/measurementDNA preparationDNA methylationSequence analysis
It has been established that one or more large double stranded DNA fragments (each 2,000 to 40,000 base pairs in size) can be captured and isolated from genomic DNA fragments using sequence specific PNA hybridization probes. Compositions and methods for enrichment of a multiplicity of long DNA sequences selected from the genome of any eukaryote are provided. Capture is performed using multiple PNA molecules with gamma-modified chiral backbones, comprising a mixture of neutral and positive chemical groups. Two or more PNA probes with covalently bound haptens, preferably biotin, target each DNA domain of interest for capture, isolation, and subsequent sequencing analysis of the multiplicity of enriched targets, including DNA methylation sequencing. The methods include enhancement of probe-DNA binding specificity through single strand binding proteins (SSB).
Owner:PETAOMICS INC
Method for rapidly identifying chromosome ploidy of cucumber horticultural variety
PendingCN120829988AMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyHybridization probe
The invention discloses a method for rapidly identifying chromosome ploidy of a cucumber horticultural variety. The method mainly comprises the following steps: preparing cucumber somatic cell interphase nuclear flakes; preparing a 5S rDNA (ribosomal deoxyribonucleic acid) probe / centromere sequence (Type-III) probe; and carrying out FISH hybridization. FISH hybridization signals of the 5S rDNA probe / centromere sequence (Type-III) probe are very stable in the whole cell cycle of the cucumber and are convenient to count, so that the chromosome ploidy of the cucumber variety can be accurately identified no matter whether the cell is in an interphase or a division phase. The invention provides a rapid and accurate identification method for chromosome ploidy identification of cucumber haploids or some growing point materials difficult to obtain root tips and the like.
Owner:NANJING AGRICULTURAL UNIVERSITY