195 results about "Repetitive Sequences" patented technology

The invention discloses novel methods and compositions for the detection of a target nucleic acid molecule in a sample. In particular, the invention provides a method of producing a probe having removed repetitive sequences comprising: (a) providing a source nucleic acid molecule containing repetitive sequences; (b) providing a driver nucleic acid molecule attached to a label and containing repetitive sequences that hybridize with the repetitive sequences of the source nucleic acid molecule; (c) hybridizing the source nucleic acid molecule and the driver nucleic acid molecule in the presence of a molecule that binds the label of step (b) wherein the repetitive sequences of source nucleic acid molecule hybridize with the repetitive sequences of the driver nucleic acid molecule to form a product; (d) subtracting the hybridized repetitive sequences of the product of step (c) by extraction with a protein dissolving solution to remove the hybridized repetitive sequences from the product; and (e) recovering the probe having repetitive sequences removed therefrom.

This method consists in making a routing automaton (50) carry out verifications on the integrity of the packets arriving at the packet switch (1), their periods of stay in the packet switch (1), their matching with the virtual paths that they claim to take as well as the routings proper, within the packet switch (1), of the datagrams that have satisfactorily undergone the verification. The routing automaton (50) is provided with a random access memory of instructed values (60) containing a table of virtual path local descriptors. The processing time of the automaton is divided into a repetitive sequence of time slots individually allocated to the different input ports (21, 22, 23) of the packet switch (1).

Recombinant bacterial triple-helical collagen-like proteins comprising two or more repetitive sequences of Gly-Xaa-Yaa yielding high-stability polymeric constructs without the need for post-translational modifications and which may incorporate one or more functional domains of biological or structural importance. The polymers are capable of high-yield production for a variety of applications

The invention relates to the technical field of biology, and relates to a human X-chromosome composite amplification system composed of 20 short serially-connected repetitive sequences and applications thereof. The composite amplification system comprises 20 pairs of primers and is capable of amplifying 19 STR sites: DXS6795, DXS6803, DXS6807, DXS9907, DXS7423, GATA172D05, DXS101, DXS9902, DXS7133, DXS9810, GATA31E08, DXS6800, DXS981, DXS10162, DXS6809, GATA165B12, DXS10079, DXS10135, and HPRTB, and one sex recognition site: Amelogenin. Five-color fluorescence labeling is adopted by the system, the size of amplification products is in a range of 85 to 450 bp, the operation is simple, and the individual recognition performance is high. The sites provided by the provided composite amplification system are highly compatible to the conventional commonly-used X-STR kits, moreover, sites with high polymorphism information are provided, and the provided composite amplification system can be applied to paternity identification related with X-chromosome, individual recognition, and auxiliary detection of X-chromosome sex-linked hereditary diseases.

The invention belongs to the field of biotechnology, and discloses a FISH (fluorescence in situ hybridization) probe, a kit and a detection method for detecting BCR/ABL fusion gene free from repetitive sequence. A BCR gene and ABL gene are used as templates to perform polymerase chain reaction on non-repetitive sequence in the BCR gene and ABL gene, the amplification product is DNA (deoxyribonucleic acid) fragments with 350-800 different base-pairs, the repetitive sequences in the BCR gene and ABL gene are eliminated so as to form the product free from repetitive sequence; after the product is in fluorescence labeling, the BCR gene and ABL gene free from repetitive FISH are obtained for the BCR gene and ABL gene FISH detection. The BCR/ABL fusion gene FISH probe obtained by the invention can be used for eliminating the repetitive sequence, the non-specific background signal of the FISH probe can be obviously reduced, and the specificity of the FISH probe is improved.

The invention relates to a polypeptide, and a production method and the application thereof, belongs to the technical field of genetic engineering, and provides the polypeptide. The polypeptide comprises n repetitions of the sequence shown in SEQ ID No.1; the n is an integer which is greater than or equal 1; when the n is the integer which is greater than or equal to 2; various repetitive sequences are directly connected with each other. The polypeptide provided by the invention has an excellent adhesion effect, has high stability in an aqueous solution, and can remove endotoxin more easily.

The invention relates to a spider dragline silk protein optimized expression method, belonging to the field of bioengineering. The method comprises the following steps: constructing high-molecular-weight recombinant spider dragline silk protein grains in Escherichia coli, and carrying out expression production, wherein the protein yield is enhanced by lowering the culture temperature in the protein expression production process; and by using the combined strategy of high-density fermentation and culture temperature lowering on the fermentation tank level, implementing the double enhancement of the cell quantity and expression level. The method can effectively overcome the defects of low expression level of the highly-repetitive recombinant spider dragline silk protein in the production fermentation process, and provides favorable reference meanings for fermentation production study and application of spider silk proteins and similar highly-repetitive sequence proteins.

The present invention provides a high-throughput sequencing method for methylated DNA, and use thereof. Particularly, the present invention provides a high-throughput sequencing method for methylated DNA, which combines methylated DNA immunoprecipitation, removal of repetitive sequences, and bisulfite treatment. The site of sequencing library will be decreased, and the cost will be reduced by using the method disclosed in the present invention.

The current invention provides for methods and medicaments that apply an oligonucleotide comprising aninosine and/or an uracile and/or a nucleotide containing a base able to form a wobble base pair, said oligonucleotide being preferably RNAse H substantially independent and being complementary only to a repetitive sequence in a human gene transcript, for the manufacture of a medicament for the diagnosis, treatment or prevention of a cis-element repeat instability associated genetic disorders in humans. The invention hence provides a method of treatment for cis-element repeat instability associated genetic disorders. The invention also pertains to a modified oligonucleotide which can be applied in a method of the invention to prevent the accumulation and/or translation of repeat expanded transcripts in cells.

An industrial robot for tending a machine includes a machine part providing a repetitive sequence of movements. The robot includes a robot controller having a program storage for storing a path of programmed positions for the robot and a path of programmed positions for the machine part, and a motion planner configured to plan the motion of the robot and the motion of the machine part based on the programmed positions for the robot and the machine part such that the motion of the robot and the motion of the machine part are coordinated with each other.

An optical filter has a multilayer thin film comprising first to i-th layers stacked in alternate layers of high and low refractive indices on a transparent substrate. Respective odd-numbered layers (Sigh refractive index layers) and respective even-numbered layers (low refractive index layers) form repetitive sequences of layers each of which cyclically changes in optical thickness throughout the multilayer tin film. Each of k-th from-the-bottom alternate layers of high and low refractive indices has such an optical thicknesses as meet the following conditional expressions (1) and (2), concurrently:

n×d=(.c/4)×[1+sin{(k−1)×}×.]  (1)

0 . . . <1   (2)

where

• .c is the center wavelength of reflection band,
• n is the ref ion index of the layer for the d-line,
• d is the physical thickness of the layer,
• . is a factor of a pitch angle represented by 2./the number of layers a one layer-stack,
• . is the rate of amplitude.

The invention discloses a DNA electrochemical sensor based on target DNA repetitive sequence self enhancement and amplification signal. A signal probe is designed by a repetitive sequence clip of a detected target chain, thus, the volume of the signal probes combined with the target sequence is increased, and a compound is formed, when a capture probe on electrode surface is hybridized with the target sequence, the amplified signal probe is combined with the electrode surface, under the affinity function between the biotin treated by end labeling of the signal probe and the avidin modified on the horseradish peroxidase, multiple enzymes are combined with a 'sandwich' structure, finally, under the efficient catalytic action of the enzymes, H2O2 is catalyzed to oxidize the TMB, the oxidative product generates an amplified reduction current signal on the electrode, and the concentration of the target DNA is judged according to the magnitude of the current signal. In the experiment, based on the characteristics of self enhancement and amplification signal of the target chain of the repetitive sequence, the target chain with two sections of repetitive sequences is detected, and the method is high in sensitivity.

The invention relates to a mutation analysis method and device for cell-free DNA (cfDNA) sequencing data. The method comprises the following steps: 1, preprocessing original sequencing data; 2, comparing the sequencing data with a reference standard genome, identifying and eliminating PCR repetitive sequences, and correcting comparison errors of sequences to obtain an accurate sequence comparisonfile; 3, identifying tumor-related somatic cell variation sites, adopting a Sentieon software TNScope tool, setting parameters to ensure that cfDNA variation sites higher than 5/10000 variation abundance can be detected, and obtaining a potential tumor-related somatic cell variation site table; and 4, analyzing and identifying the highly credible low-frequency variation, and finally obtaining an expected positive variation analysis result. The method can achieve ultrahigh mutation sensitivity detection and high verification accuracy.

A packet detection technique is disclosed in which an average correlation signal is generated representative of the match between a repetitive sequence of symbols; an average power signal is generated representative of the average power in the sequence of symbols; a scaled magnitude of the average correlation signal scaled by a first predetermined scale factor is produced; and one of the average power signal and scaled magnitude of the average correlation signal are multiplied by the second scale factor and compared to determine whether there is a match between a repetitive sequence of symbols.

The invention supplies a fluorescence labeling verification system by compounding and expanding 14 short series repeating sequence locus to take personal identification and paternity test. The specific compounding method controls the expanding product in the range of 400 basic groups, and only labeling three fluorescence of the compounding expanding of the 14 locus would be realized. The invention takes a deep research to the gene characteristic of the 14 locus and the allele in five minorities in China, and supplies the compounding expanding verification system. The agent box suited for Chinese throng is researched.

The invention provides an AETCA (anchored-extension and telomeric complements amplification) detection reagent kit for telomerase and a detection method. The reagent kit comprises an anchored PCR (polymerase chain reaction) tube, a probe BT (SEQ ID No.2), PCR primer, denaturing lysis buffer, extension hybridization buffer, PCR buffer and washing buffer, wherein telomerase primer ATS (SEQ ID No.1) are fixed in the anchored PCR tube. By utilizing a part of probe sequence (the probe BT) complementary with a telomere repetitive sequence as a template, the probe is combined on the telomere repetitive sequence synthesized by the telomerase in a multicopy manner, and accordingly, amplification of the probe BT can be kept in efficient fixed length though the length of the telomere repetitive sequence is not constant, and the defects of the TRAP (tartrate resistant acid phosphatase) method are overcome. The detection method is convenient and easy to operate, sensitivity and specificity of activity detection of the telomerase can be improved, and the detection method is applicable to detection of various sources including cells or tissues of clinical samples such as sputum.

The invention provides a kit for realizing multiple amplification of 21 short tandem repeats by adopting degenerate primers, and belongs to a multiple amplification system for simultaneously analyzing a plurality of STR gene loci. 21 gene loci are amplified in a multiple amplification system simultaneously, and are divided into the following four groups: the first group: D19S433, TH01, D21S11, D18S51 and Penta E; the second group: D3S1358, D13S317, D7S820, D5S818, CSF1PO and Penta D; the third group: Amelogenin, vWA, D8S1179, TPOX, D6S1043 and D1S1656; and the fourth group: D16S539, D12S391, D2S1338 and FGA. Specific primers are respectively designed on flanks of a gene loci repetitive sequence, so that the balance of the peaks of the fragments inside the gene loci groups achieves 40% or above, the four gene loci groups are subjected to multiple amplification, and the concentration of the primer of each gene locus is regulated, so that the integral balance of the peaks of the gene loci achieves 30% or above. With the kit, a balanced and stable multiple amplification result is easily obtained, so that the kit belongs to a multiple amplification system with low cost and high efficiency.

Provided are an improved procedure and associated hardware to allow a satellite to switch antenna coverages according to predefined repetitive sequences, and to align switching of the antenna sequence with ground data sequence switching. The disclosed technique enables a beam hopping sequence to be changed without losing connectivity between the satellite and ground segment.

The present invention relates to a vertical, three-dimensional semiconductor device (10) comprising a source layer (102) on a substrate (104), a horizontal stack of layers (106) of a repetitive sequence on the source layer (102), each sequence comprising an electrically isolating layer (108) and an electrically conductive gate layer (110). An electrically isolating layer (108a) of the stack of layers (106) is in contact with the source layer (102), a vertical channel structure (112) extending through the horizontal stack of layers (106), a metal drain (114) being arranged above the horizontal stack of layers (106) and the vertical channel structure (112), the source layer (102) being arranged to inject charge carries into the vertical channel structure (112) and the metal drain (114) being arranged to extract charge carries from the vertical channel structure (112). The semiconductor device (10) may be junction-less and the vertical channel structure (112) may comprise a high mobility semiconductor material.