95 results about "Acid molecule" patented technology

The invention discloses a salicylic acid-cyclodextrin-amino acid ternary inclusion compound and a preparation method thereof. The salicylic acid-cyclodextrin-amino acid ternary inclusion compound is formed by salicylic acid, amino acid and a cyclodextrin derivative through non-covalent bond interaction, the cyclodextrin derivative is a cross-linked beta-cyclodextrin polymer, salicylic acid molecules are included in a cavity of the cross-linked beta-cyclodextrin polymer, and the cross-linked beta-cyclodextrin polymer is a cross-linked beta-cyclodextrin polymer. And meanwhile, guanidyl or carboxyl of an amino acid side chain interacts with hydroxyl outside the cross-linked beta-cyclodextrin polymer or an exposed part of an included salicylic acid molecule. According to the invention, a cross-linked beta-cyclodextrin polymer is used, arginine is introduced as a synergist, a salicylic acid-cross-linked beta-cyclodextrin polymer-arginine ternary clathrate compound is constructed, and through charge neutralization and hydrogen bond network synergistic effect, clathration efficiency is significantly improved, and water solubility of salicylic acid is improved.

The invention discloses a lipid compound for delivering a therapeutic agent as well as a preparation method and application of the lipid compound, and particularly discloses a compound shown in a formula I or pharmaceutically acceptable salt, stereoisomer, tautomer, solvate, chelate, non-covalent complex or precursor of the compound, the ionizable lipid compound can effectively deliver nucleic acid molecules, small molecule compounds and other drugs, and through comparison, the lipid nanoparticles are good in particle size distribution and high in encapsulation efficiency, the delivery effect can be obviously better than that of contrast lipid nanoparticles, and the requirement of in-vivo delivery can be met. (I)

The present invention provides a method for detecting and identifying molecular chirality based on a composite plasmon chiral nanostructure. The present invention constructs a composite plasmon chiral nanostructure using chiral nanofibers and gold nanorods as basic components, and achieves molecular chirality recognition and detection through plasmon chiral optical amplification. The excellent plasmon circular dichroism optical properties of the composite nanostructure can convert the host-guest chiral recognition interaction occurring at the fiber interface into an asymmetric plasmon chiral optical amplification signal. This technology can detect and identify the chiral enantiomers of amino acid molecules in about 1 minute, with a sensitivity of 10 ‑6 mol / L. This structure can also be used for quantitative analysis of molecular enantiomeric excess (ee), with a detection sensitivity of ±0.05. Furthermore, given that the chiral host-guest complexes involved in this technology are primarily based on hydrogen bonding interactions, the chiral fiber host can be used as a universal substrate for the detection of chiral amino acid molecules.

Short interfering nucleic acid (siNA) molecules, compositions, and methods containing modified nucleotides and their uses are described.

The invention discloses synthesis of 5-nucleotide phosphorodithioamide and application of the 5-nucleotide phosphorodithioamide in oligonucleotide. The oligonucleotide modified by the phosphorodithioamide comprises nucleotide structural units shown in a formula I or a formula II,..., the formula I,..., the formula II. According to the invention, through specific limitation of the structure of a nucleotide dithiophosphoramide monomer, the prepared oligonucleotide comprises a 5-nucleotide dithiophosphoramide structural unit. Compared with a traditional oligonucleotide molecule, the oligonucleotide molecule modified by nucleotide dithiophosphoramide shows better enzymatic degradation resistance and pharmaceutical stability. In addition, the binding performance between the modified oligonucleotide molecules and environmental ions can be improved through nucleotide dithiophosphamide modification, and a novel chemical modification strategy is provided for research, development and application of oligonucleotide drugs.

This tin alloy plating solution comprises: (A) a soluble salt that contains at least a stannous salt; (B) a soluble salt of a metal that is nobler than tin; (C) a leveling agent that is composed of an alkanesulfonic acid which contains 9-18 carbon atoms in each molecule or a salt thereof; (D) a free acid; (E) a nonionic surfactant that contains one or more phenyl groups in each molecule; and (F) an EO / PO block polymer that has an EO group at an end. The EO ratio in the EO / PO block polymer is within the range of 20 mol% to 50 mol% inclusive, and the molecular weight of the EO / PO block polymer is within the range of 1,500 to 3,500 inclusive.

This invention discloses a closed-loop acid-base recovery system and method for the resource recovery of weak organic acid salt wastewater. The front-end neutralization unit neutralizes fresh organic acid wastewater with alkali solution to generate a weak organic acid salt solution to be treated, which is then fed into a diffusion dialysis unit. The diffusion dialysis unit is divided into a diffusion liquid channel and a receiving liquid channel by a diffusion dialysis anion exchange membrane, achieving pre-enrichment of organic acid ions through concentration difference. The bipolar membrane electrodialysis unit, driven by a DC electric field, breaks down the weak organic acid salt into organic acid and alkali solution. The inlet of the acid chamber is connected to the diffusion liquid outlet of the diffusion dialysis unit, and all the produced dilute organic acid solution is transported to the diffusion dialysis unit as an acid receiving liquid for recycling. All the regenerated alkali solution produced in the alkali chamber is transported to the front-end neutralization unit for recycling. The receiving liquid outlet of the diffusion dialysis unit is connected to an acid product collection unit. This invention fundamentally inhibits the back diffusion of weak acid molecules, achieving efficient recovery of organic acids and recycling of alkali solution.

The present invention relates to a nucleic acid conjugate and methods of making and using the same, comprising: a first nucleic acid molecule, a second nucleic acid molecule, and a linker, the first nucleic acid molecule being linked to the second nucleic acid molecule by the linker, the linker linking the first nucleic acid molecule and the second nucleic acid molecule by a phosphodiester (p) or phosphorothioate (s) linkage, the linker being selected from the structures shown in Formula (I):

The invention relates to the technical field of biological medicine, and particularly discloses synthesis and application of oligonucleotide with threose nucleic acid 3-oxygen at the tail end indirectly connected with thiophosphoric acid. The chemical modification strategy comprises the following steps: O < 3->-oxygen of threose nucleic acid is connected with thiophosphoric acid through a spacer group, and O < 2->-hydroxyl is combined with a nucleotide monomer of phosphoramidite; carrying out solid-phase synthesis on the threose nucleic acid O2 '-phosphoramidite monomer to construct an oligonucleotide molecule; the oxygen of the threose nucleic acid at the terminal of the oligonucleotide is linked to the thiophosphoric acid through a spacer group, and since the type of phosphonic acid does not belong to a substrate of phosphatase, the modified oligonucleotide can resist exonuclease degradation and improve its in vivo biological activity.

The present invention relates to a siRNA conjugate and a preparation method and use thereof, the siRNA conjugate comprises: a first nucleic acid molecule, a second nucleic acid molecule and a linker, the first nucleic acid molecule is connected to the second nucleic acid molecule through the linker, the linker is respectively connected to the first nucleic acid molecule and the second nucleic acid molecule through phosphate (p) or thiophosphate (s), the linker is selected from at least one of A1, A2, A3, A4, A5, A6 or A7, or any combination thereof.

The embodiment of the invention discloses a controllable preparation method of an alpha-Fe2O3 nanostructure, which comprises the following steps: S1, stirring and mixing amino acid and a NaOH aqueous solution to obtain a first mixed solution; s2, mixing and stirring a FeCl3. 6H2O aqueous solution and the first mixed solution to obtain a second mixed solution; s3, mixing and stirring a urea aqueous solution, a polyethylene glycol-1500 aqueous solution and the second mixed solution to obtain a third mixed solution; and S4, carrying out a hydrothermal reaction on the third mixed solution, and separating a reaction product to obtain the alpha-Fe2O3 nanostructure formed by regulating nanowire assembly through amino acid molecules. According to the method, amino acid molecules are used for regulating and controlling the growth process of the nanowires, the alpha-Fe2O3 nanostructure is assembled, morphology regulation and control of the alpha-Fe2O3 nanostructure can be achieved through the concentration of the amino acid, and the nanostructures in different shapes such as cubes, nanowires and oval particles are obtained.

The invention discloses a preparation method of a chiral dendronized diacetylene acid molecule and a supramolecular chiral assembly of the chiral dendronized diacetylene acid molecule, and relates to the field of intelligent chiral materials.The preparation method comprises the steps that firstly, tree-shaped alkoxy ether is combined with PCDA through lysine, the tree-shaped alkoxy ether serves as a hydrophilic element, lysine serves as a chiral source and can provide a large number of hydrogen bonds, the PCDA serves as a hydrophobic element, and the supermolecular chiral assembly of the chiral dendronized diacetylene acid molecule is obtained; chiral dendronized diacetylene acid molecules are designed and synthesized; then, supermolecule chiral assembly is carried out through driving of non-covalent interaction, and supermolecule chiral assembly is regulated and controlled through temperature and light; furthermore, the supramolecular chiral assembly is subjected to optical topological polymerization reaction to prepare the valence-donating chiral polymer. The invention opens up a new way for creating a novel intelligent chiral nano material with a special structure and excellent characteristics.

The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and / or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.

The application provides a modeling method, device, computer readable storage medium and processor. The method comprises the following steps: determining an initial number of acid molecules according to the concentration of a photoacid generator and a Poisson generator, determining an initial number of base molecules according to the concentration of a base quencher and a Poisson generator, and determining an initial number of protected groups in a polymer resin according to the concentration of the polymer resin and a Poisson generator; in the determining step, determining the number of protected groups at the next moment according to the number of protected groups at the current moment, determining the number of acid molecules at the next moment according to the number of acid molecules at the current moment, and determining the number of base molecules at the next moment according to the number of base molecules at the current moment; the determining step is repeated for multiple times until the next moment of the current time is the ending moment of the photoresist baking. The method solves the technical problem that the randomness of molecules is ignored in the prior art when performing nanoscale photoetching modeling.

The application discloses a reversible single-molecule switch based on local cation regulation and belongs to the technical field of molecular electronics. The application is based on the crack junction technology of an electrochemical scanning tunneling microscope. The distribution of local cations in an interface double electric layer is changed by controlling an electric potential, so that the state of a carboxylic acid molecule is influenced. The contact action of the carboxylic acid molecule and a gold needle tip is adjusted, and a single-molecule switch is realized. The electric potential is-0.5-0V. The application proves the influence of local cations in an electrochemical interface on carboxyl molecules, and opens up a new way for realizing the practical application of a reversible single-molecule switch through a gate electrode.

The present invention relates to a method for determining the sequence of a nucleic acid molecule. In particular, the present invention provides: i. a nucleic acid molecule comprising a 5' region and a 3' region, wherein the 5' region and the 3' region are covalently linked by a nucleotide sequence to which a primer can bind, and the base recognition in one of the 5' region or the 3' region and the base recognition in the other region together independently provide information regarding the base recognition at the corresponding locus in the original nucleic acid molecule, and the molecule further comprises: - one adapter at the 5' end of the molecule; - one adapter at the 3' end of the molecule; ii. sequencing the molecule provided in step (i) using at least two different primers, e.g., at least three different primers, preferably at least four different primers, wherein at least two different primers, e.g., at least three different primers, preferably at least four different primers, bind to at least three different regions, preferably at least four different regions in the nucleic acid molecule provided in (i): 1. At least one of the primers binds at least partially to at least a portion of the adapter at the 5' end of the molecule, thereby sequencing at least a portion of the 5' region of the nucleic acid molecule provided in (i); 2. The present invention provides a method comprising: 3. At least one of the primers at least partially binds to a region of a nucleotide sequence covalently linking the 5' and 3' regions of the nucleic acid molecule provided in (i), thereby enabling sequencing of at least a portion of the 3' region of the nucleic acid molecule provided in (i); 4. At least one of the primers at least partially binds to at least a portion of the adapter at the 3' end of the molecule, thereby enabling sequencing of at least a portion of the 3' region of the nucleic acid molecule provided in (i); and / or 5. At least one of the primers at least partially binds to a region of a nucleotide sequence covalently linking the 5' and 3' regions of the nucleic acid molecule provided in (i), thereby enabling sequencing of at least a portion of the 5' region of the nucleic acid molecule provided in (i).

This application provides a glycosyl-modified fusion protein, a nucleic acid molecule, an expression vector, a host cell and use thereof. A first aspect of this application provides a glycosyl-modified fusion protein including a murine Fc variant and a polypeptide antigen, where the murine Fc variant is obtained by subjecting a murine Fc fragment to amino acid mutation and non-mammalian glycosylation-modification; the murine Fc variant includes at least one of alanine at position 223, alanine at position 228, alanine at position 230, leucine at position 330 and glutamic acid at position 332; the non-mammalian glycosylation-modification excludes sialic acid-modification; the position numbering is performed according to the EU numbering system; the murine Fc variant enhances the binding of the murine Fc fragment to DC cells and DC activation, including the proliferation and activation of specific T cells.

Provided herein are compositions and methods for amplifying long nucleic acid molecules. In particular, provided herein are compositions and methods for amplifying long nucleic acid molecules that are mixed in complex samples, for example, complex biological samples.

This invention discloses an interchain disulfide bond Cys A24 -Cys B23 H2Relaxin derivatives with thioether bonds as alternatives and their synthetic methods, wherein the structural formula of the H2Relaxin derivatives is shown below: This invention first utilizes diaminodioic acid molecules to synthesize interchain disulfide bonds (Cys) in a single solid-phase process using an N-fluorenemethyloxycarbonyl (Fmoc) solid-phase polypeptide synthesis method. A24 -Cys B23 The folded refolding precursor of H2Relaxin derivatives with thioether bonds as substitutes was then efficiently obtained via one-step oxidative folding and refolding to yield interchain disulfide bonds (Cys). A24 -Cys B23 The H2Relaxin derivative is replaced by a thioether bond.

The invention discloses a Co-Ni bimetal sandwich polyacid crystalline material as well as a preparation method and application thereof. The molecular formula of the material is [Ni (L) 2 (H2O) 3] 2 [Co2Ni2 (GeMo10O36) 2 (H2O) 4]. 7.5 H2O. L is 1-(4-formyl benzyl)-[4, 4 '] bipyridine chloride; the crystal system is triclinic; the space group is P-1; the cell parameters are as follows: alpha is equal to 103.9140 (10) degrees, beta is equal to 91.5310 (10) degrees, gamma is equal to 119.2830 (10) degrees, and Z is equal to 1. The preparation method comprises the following steps: 1, dissolving germanium oxide, ammonium molybdate, a cobalt salt, a nickel salt and a ligand L in water, and regulating the pH value of the solution with an acid regulator to obtain a reaction solution; and 2, reacting the reaction liquid in the step 1 in a high-temperature closed environment, and cooling to room temperature after the reaction is finished, so as to obtain the Co-Ni bimetal sandwich polyacid crystalline material. According to the crystalline material synthesized by the method, polyacid molecules are directly connected with Co-Ni bimetal sites, electrons are directly transferred to the metal sites, and loss of the electrons is avoided, so that the catalytic performance is greatly improved.

The invention proposes a device for analyzing nucleic acid molecules (M), comprising: —a bead (20), on which one molecule can be anchored at one end, —a surface (520), on which the molecule can be anchored at the other end, —an actuator (30), adapted to cause the bead to move relative to said surface in one direction, —a sensor (50), adapted to measure a distance between the bead and the surface, the device further comprising a well (11), having an axis (X-X) extending along the direction of motion of the bead and a bottom (110) formed by said surface, said well being filled with electrically conductive solution (40), and receiving the bead, the sensor being adapted to measure an impedance of the well, depending on a distance between the bead and the surface, to determine, the distance between the bead and the surface.

The application discloses a kind of nanometer thickness square acid MOF film and preparation method thereof, and preparation method includes: preparation cationic surfactant, square acid, sodium hydroxide, metal salt is dissolved in water respectively and is matched into 1mg / mL solution;Square acid MOF Langmuir film is assembled using Langmuir film instrument, and square acid MOF film is transferred to solid substrate using Langmuir-Blogget method, to obtain nanometer thickness square acid MOF film;The application is induced by cationic surfactant to interface aggregation of water-soluble negative square acid molecule, interface pressure is reasonably controlled using Langmuir film preparation technology, adjust the distance between surfactant and interface charge density, then control the interface density of square acid ligand molecule and film thickness induced by it, prepare film thickness 2nm, size is centimeter level, defectless MOF film material.

Disclosed are methods for depleting unmethylated nucleic acid molecules, thereby enriching for target sequences of interest in a sample (e.g., a sample obtained from a subject). Such methods are useful for enriching for a signal in a sample, such as a signal informative for determining presence or absence of cancer in the sample.

The invention provides a protein phosphorescent material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving arylboronic acid molecules in water, mixing with a protein solution and an alkaline substance to obtain a mixed solution, carrying out dehydration condensation reaction on the mixed solution to obtain phosphorescent ink, and preparing the phosphorescent ink into the protein phosphorescent material. The preparation conditions are mild, the reaction is rapid, the protein provides a rigid environment to limit molecular thermal power such as vibration and rotation of luminescent molecules so as to inhibit non-radiative transition, and long-life organic room-temperature phosphorescence is realized. Based on the excellent processability and degradability of the fibrous protein, the prepared phosphorescent material can be processed into various material forms and is environment-friendly. In addition, phosphorescence emission is adjusted through stimulation of external conditions such as water, methanol, UV and temperature, and display and hiding of phosphorescence patterns are achieved.

A method for processing polynucleic acids is described, comprising: a) providing a solid support containing ligands, wherein at least a portion of the ligands contain a cyclic amine group having more than six ring members; exposing the solid support to polynucleic acid molecules in a buffer having a pH less than 5.5 to bind at least a portion of the polynucleic acid molecules to the ligands; exposing the solid support having the bound polynucleic acid molecules to a pH greater than 6 to release a portion of the bound polynucleic acid molecules from the ligands of the solid support, optionally retaining a portion of the polynucleic acid molecules bound to the solid support; and utilizing the released portion of the bound polynucleic acid molecules and / or the retained portion of the polynucleic acid molecules bound to the solid support, or a suspension thereof. A solid support containing ligands bound to a solid support (e.g., magnetic beads), wherein at least a portion of the ligands contain a cyclic amine group having more than six ring members, and a kit containing such a solid support are also described.

This invention belongs to the field of novel glycoconjugates and provides a method for preparing a sialic acid-gold nanomaterial composite. The method includes the following steps: S1: synthesizing a sialic acid ligand functional molecule; S2: synthesizing a PC molecule; S3: replacing the stabilizing molecule on the surface of the gold nanomaterial with a modified molecule under the action of a promoter to obtain a surface-modified material intermediate; S4: activating the surface carboxyl groups of the surface-modified material intermediate, reacting it with the PC molecule and the sialic acid ligand functional molecule, and connecting them through amide bonds. This invention provides a method for creating a sialic acid-gold nanocomposite material with a novel surface structure. The sialic acid molecule is obtained by modifying the structure of natural sialic acid molecules and can specifically recognize and bind to the SARS-CoV-2 S protein. The superhydrophilic properties of the PC group are utilized to reduce non-specific interference of complex environments on the material, while maintaining the biological effects of the sialic acid-gold nanocomposite material.

The application discloses a high-stability small-particle-size nucleic acid-gold nanocomposite and a synthesis method thereof, and belongs to the technical field of functional nanomaterial preparation, and the synthesis method comprises the following steps: under the conditions of heating and stirring, a reducing agent solution is added into a boiling chloroauric acid aqueous solution in batches, a solution of a functional nucleic acid molecule with a PolyA sequence at the terminal is added after boiling again, an acetic acid solution is added when the color of the reaction system is changed from light pink or light purple to dark, and the high-stability small-particle-size nucleic acid-gold nanocomposite is prepared after the reaction is completed; the application provides a brand-new "one-pot" synthesis strategy, discards complicated multi-step separation operation, realizes the synthesis of gold nanoparticles and the surface nucleic acid functionalization of the gold nanoparticles in one step in the same reaction system, the preparation process is simple, the product is uniform in particle size, good in dispersity, free from a plasmon resonance absorption peak, and has excellent long-term storage stability without depending on any exogenous polymer protective agent.

To provide methods and systems for analyzing nucleic acid molecules.SOLUTION: Processes and materials to detect cancer from a biopsy are described. In some cases, cell-free nucleic acids can be sequenced, and the sequencing result can be utilized to detect sequences derived from a neoplasm. Detection of somatic variants occurring in phase can indicate the presence of cancer in a diagnostic scan and a clinical intervention can be performed. The present disclosure provides methods and systems for analyzing cell-free nucleic acids (e.g., cfDNA, cfRNA) from a subject. Methods and systems of the present disclosure can utilize sequencing results derived from the subject to detect cancer-derived nucleic acids (e.g., ctDNA, ctRNA) for, e.g., disease diagnosis, disease monitoring, or determining treatments for the subject.SELECTED DRAWING: None