18 results about "Gold nanorod" patented technology

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The application relates to a surface enhanced Raman detection technology, in particular to a spherical composite SERS substrate and a preparation method and application thereof. The method comprises the following steps: adopting a micro flow injection pump to drop a solution containing silver nanowires and gold nanorods into liquid nitrogen, and then performing freeze drying; wherein, a gun head of the micro flow injection pump is a hydrophobic gun head; the concentration of the silver nanowires in the solution containing the silver nanowires and the gold nanorods is 15-40 mg / mL. The spherical composite SERS substrate prepared by the method has a spherical structure formed by silver nanowires and metal nanorods, the spherical skeleton structure is formed by interweaving silver nanowires into a net, and the metal nanorods are loaded on the silver nanowires, and the spherical composite SERS substrate can realize detection of rhodamine 6G without introducing a modifier.

The present invention relates to: a biosensor for simultaneous multiplex detection of target nucleic acid molecules, the biosensor comprising gold nanorods with immobilized ssDNA and ssRNA; and a composition for diagnosing cancer using the biosensor. The present invention enables rapid simultaneous multiplex detection of cancer-associated cfDNA and miRNA, which are target nucleic acid molecules, to be performed with high accuracy and sensitivity, and thus can be advantageously used in the field of cancer diagnosis.

The embodiment of the application belongs to the technical field of immunological detection in vitro diagnostic reagents, and relates to a preparation method of a detection card for detecting a novel coronavirus, comprising the following steps: preparing a gold nanorod solution; mixing the gold nanorod solution with a novel coronavirus monoclonal antibody to prepare a gold nanorod-antibody complex solution; preparing a binding pad and a sample pad through the gold nanorod-antibody complex solution; performing antibody coating on a nitrocellulose membrane to obtain a reaction pad coated with antibodies; assembling the binding pad, the sample pad and the coated membrane to obtain a colloidal gold immunochromatography test strip; and assembling the colloidal gold immunochromatography test strip and a shell to obtain the detection card. The application effectively improves the sensitivity of detection.

The application discloses a preparation method of a flexible carbon paper / multitwin gold nanostructure surface Raman enhancement (SERS) substrate, which comprises the following steps: S1, a three-electrode system is built by taking dilute sulfuric acid as an electrolyte, gold nanorods as a counter electrode, Ag / AgCl as a reference electrode, and carbon paper as a working electrode; S2, gold on the counter electrode is deposited onto the surface of the working electrode by using a linear sweep voltammetry method to form a multitwin gold nanostructure; and S3, the working electrode on which the multitwin gold nanostructure is deposited is cleaned with ultrapure water, and after drying, a flexible carbon paper / multitwin gold nanostructure SERS substrate is obtained. The multitwin gold nanostructure with excellent SERS performance is generated by directly transferring gold from the gold nanorod counter electrode to the carbon paper working electrode through a direct electrotransfer method on the carbon paper, and is used as the SERS substrate.

A photoelectric conversion element including a thin film composed of a transition metal dichalcogenide and formed on a base material. In the present invention, a surface of the thin film composed of the transition metal dichalcogenide is modified with at least any nanorod particles of Au nanorod particles composed of Au and Ag nanorod particles composed of Ag. An average aspect ratio of the Au nanorod particles is 3.0 or more and 12.0 or less, and an average aspect ratio of the Ag nanorod particles is 3.0 or more and 13.0 or less. In the invention, the sensitivity to light having wavelengths in the near-infrared region improves by a sensitizing action attributed to localized surface plasmon resonance that is developed by the Au and Ag nanorod particles.

The application provides a kind of bionic nano immune base material and its preparation method and application, wherein the bionic nano immune base material includes PDMS@AgNPs@ZIF-67 bionic immune base and gold nanorod immune probe, its preparation method includes the following steps: (S10) the preparation of ZIF-67 nano material;(S20) the preparation of PDMS@AgNPs@ZIF-67 bionic polymer material;(S30) the preparation of PDMS@AgNPs@ZIF-67 bionic immune base material;And (S40) the synthesis of gold nanorod immune probe, by studying the construction of SERS base on MOFs modified bionic base, which has both synergistic effect, and has the ability of anchoring molecule, improve the efficiency and sensitivity of prostate cancer examination, it has important significance to improve the detection efficiency of PSA.

This invention relates to a chiral device for identifying enantiomers of amino acids, which can be used to distinguish different enantiomeric configurations of the same amino acid. Light is incident from a substrate along the z-direction, passing sequentially through a glass substrate and a gold nanorod composite structure. The gold nanorod composite structure consists of a double L-structure and disjointed tandem nanorods, making full use of gaps and structural asymmetric absorption to achieve a circular dichroism signal of 0.147 in the near-infrared 0.6–1.7 μm band. The refractive index sensitivity at the peak of the circular dichroism spectrum can reach 324 nm / RIU, enabling the identification of amino acid enantiomers.

This invention belongs to the field of biodetection technology and discloses a fluorescence detection method for the prostate cancer biomarker PCA3 based on gold nanorods and CRISPR Cas12a amplification. This invention provides a detection kit for the ultrasensitive detection of the long non-coding RNA PCA3, a tumor marker for prostate cancer. This kit utilizes the paracleavage property of CRISPR Cas12a activated by the target sequence to cleave the fluorescent reporter probe, releasing the fluorophore. This removes the signal quenching effect of the gold nanorods on the fluorophore, restoring the fluorescence signal and achieving ultrasensitive detection of the target sequence. The limit of detection (LOD) of this kit is as low as 1.65 pM, achieving highly selective quantitative detection of PCA3.

The disclosure relates to particle-based assays for the detection of analytes. Using various combinations of particular technologies, including gold nanorods, silver nanoparticles, gold / silver nanoshells, gold / silver nanocages and nanobubble detection, enhanced detection limits can be achieved across a large range of analytes.

The application belongs to the technical field of biological medicine and nanomaterials, and particularly relates to a nano system for targeted photothermal-chemotherapy synergistic treatment of abdominal aortic aneurysm as well as a preparation method and application thereof. The system is a mesoporous polydopamine-coated gold nanorod cluster (AuM) nanoparticle co-loaded with one or more traditional Chinese medicine ingredients and modified by c(RGDfK) cyclic peptide. The preparation comprises the following steps: taking chloroauric acid and dopamine hydrochloride as raw materials, synthesizing AuM in the presence of a reaming agent and a template agent by a one-step method; further loading a large amount of drugs in the mesopores to obtain drug-loaded nanoparticles; and finally grafting a targeting peptide on the surface of the nanoparticles to obtain the final product. The system has the characteristics of targeting macrophages and smooth muscle cells, NIR-Ⅱ photothermal conversion and photothermal response drug release. The in-vitro and in-vivo experimental results show that the system can realize local photothermal treatment and precise drug release under NIR-Ⅱ light, and inhibit aortic dilation through various cell behaviors, thereby providing a nano preparation with clinical transformation potential for precise treatment of abdominal aortic aneurysm.

The application discloses a gold nanorod enhanced photothermal elastic gas detection system and a detection method, which are used for improving the universality of the gas detection system. The system comprises an adjustable laser, a gas cell and a quartz tuning fork. The light receiving area of the quartz tuning fork is arranged at the corresponding position of the modulated laser output by the adjustable laser. The light receiving area is provided with a photoelectric conversion film coating. The photoelectric conversion film coating comprises gold nanorod material deposited on the surface of the quartz tuning fork. The longitudinal localized surface plasmon resonance absorption peak of the gold nanorod material is matched with the absorption wavelength band of the to-be-detected gas. When the modulated laser emitted by the gas cell irradiates the photoelectric conversion film coating, the gold nanorod material enhances the absorption of the modulated laser through the localized surface plasmon resonance effect and converts the modulated laser into heat energy. The heat energy generated by the photoelectric conversion film coating is transmitted to the quartz tuning fork, so that the quartz tuning fork generates thermal elastic deformation and excites the quartz tuning fork to vibrate and output a gas concentration electric signal.

This invention relates to a localized hydrogel dressing with glycemic control, antioxidant, photothermal antibacterial properties, and its preparation method and application. The composition of the localized glycemic control, antioxidant, photothermal antibacterial hydrogel dressing includes: a nanoscale dumbbell structure Au-CeO2 composed of gold nanorods and cerium oxide spheres at both ends, and glucose oxidase (GO). x And a reactive oxygen species (ROS) sensitive hydrogel matrix for encapsulating the Au-CeO2 and glucose oxidase.

This invention discloses a Raman immunoassay probe based on gold nanorods, comprising gold nanorods, a signal labeling molecule modified on the gold nanorods, and a microcystin-LR (MC-LR) specific monoclonal antibody molecule. The inventors also established a method for preparing the corresponding Raman immunoassay probe. Based on this, the inventors developed a test strip for MC-LR microcystin detection based on a gold nanorod SERS immunoassay probe. It uses gold nanorods as the SERS labeling substrate, enhancing the Raman signal to produce a stronger and more stable signal, significantly improving detection sensitivity and signal reproducibility. Simultaneously, the combination of highly specific immunorecognition elements ensures the method's anti-interference ability and accuracy in complex matrices, thus providing a practical solution for achieving highly sensitive, stable, and quantitative rapid detection of MC-LR, suitable for rapid on-site screening and quantitative detection of trace MC-LR in water and aquatic products.

This invention discloses chiral gold nanoflowers, their preparation method, and applications. The method includes the following steps: S1, preparing a growth solution; adding deionized water, chloroauric acid aqueous solution, and ascorbic acid aqueous solution to a hexadecyltrimethylammonium bromide aqueous solution and mixing to obtain a growth solution; S2, introducing chiral molecules; injecting chiral molecules L-cysteine ​​or D-cysteine ​​into the growth solution and mixing to obtain a chiral induced growth system; S3, seed-induced growth; adding a gold nanorod solution to the chiral induced growth system and mixing; allowing the reaction to stand, and growing to obtain crude chiral gold nanoflowers. The preparation method uses gold nanorods as seed crystals, and the excessive growth of gold nanoflowers is induced in situ by chiral cysteine ​​molecules. The reaction conditions are mild, the operation is simple and easy to control, the product exhibits significant chiral characteristics, and high reproducibility. Chiral gold nanostructures can expand applications in catalysis, biosensing, chiral recognition, optical devices, and other fields, showing good application prospects and practical application value.

The application discloses a controllable assembly method of nanoparticles. The method is realized by complexing carboxyl-modified pillar[5]arene (CP[5]A) and pyrene butyric acid grafted organic imidazole salt through host-guest interaction. Through a two-step simple substitution reaction, 1-methyl-3-(10-(4-pyrene-1-butanoic acid)-chrysene-1H-imidazole bromide and derivatives containing different carbon numbers are synthesized, which are mixed with the reported CP[5]A to obtain the assembly system. By adjusting the concentration of the host and guest organic matters and the chain length of the guest organic matter, AuNPs can be controllably assembled and form well-defined one-dimensional and two-dimensional structures, especially the fine assembly of AuNPs can be realized. The assembly system has high stability and can exist stably for months. Meanwhile, the assembly system has good universality and can be used to assemble different types of nanoparticles, such as silver nanoparticles, silver nanosheets, iron gold nanoparticles and gold nanorods, and is a simple and universal assembly platform.

The application belongs to the field of material chemistry and molecular electronics, and discloses a preparation method of gold nanorod assembly. The method comprises the following steps: providing a dispersion system containing a plurality of gold nanorods in a liquid medium; introducing a heterocyclic assembly molecule anchored on the surface of the gold nanorod into the dispersion system, wherein the heterocyclic assembly molecule contains a structural rigid aromatic heterocycle, a sulfur-containing anchoring group forming a coordination bond with the gold surface, and a group or atom capable of forming a hydrogen bond; the gold nanorod assembly contains at least two gold nanorods and the heterocyclic assembly molecule on each gold nanorod, and the two gold nanorods are connected by the hydrogen bond supramolecular interaction between the complementary functional groups of the heterocyclic assembly molecules. The preparation of the gold nanorod assembly with high yield and linear orientation is realized by using the heterocyclic assembly molecule.

This invention discloses a photothermal-triggered reusable drug delivery microneedle patch based on phase change materials, its preparation method, and its applications, relating to the field of pharmaceutical technology. The microneedle patch comprises several microneedles, each containing a drug-loaded matrix, a therapeutic drug, and photothermal nanoparticles. The drug-loaded matrix comprises a phase change material with reversible solidification-melting properties, capable of melting under photothermal triggering conditions and reverting to solidification after the photothermal triggering conditions disappear. The melting point of the phase change material is between 38°C and 45°C. The phase change material includes higher fatty acids and polyethylene glycol derivatives, and the photothermal nanoparticles include gold nanorods, graphene oxide, and polypyrrole nanoparticles. This invention proposes a microneedle patch using near-infrared light as a trigger signal and a phase change material with reversible solidification-melting properties. Through the melting process of the same phase change material under photothermal triggering, reusable drug delivery is achieved, overcoming the technical limitations of existing microneedles that only allow for "single-use, continuous drug release."