702 results about "Gold nanorod" patented technology

The invention discloses a top silver-cladding gold nanorod array and a preparation method and application thereof. In the array, a gold film is provided with a gold nanorod array of which the top end is cladded with silver nanoparticles with the diameters of between 60 and 90nm, and the bottom surface is provided with an organic matter substrate with the thickness of between 50 and 100 mu m. The method comprises the following steps of: evaporating the gold film on one side of a nano through-hole alumina template, and growing the gold nanorod array in the hole of the template, particularly coating a liquid organic matter on the gold film on one side on which the gold nanorod array does not grow; evaporating the gold film on one side after the liquid organic matter is cured; placing the alumina template of which the hole is deposited with the gold nanorod array and the gold film is coated with the organic matter in acid or alkali solution; etching off the template; and evaporating silver on the top of the gold nanorod array by film evaporating technology to obtain the top silver-cladding gold nanorod array. A trace amount of organic matter rhodamine or 2,3,3'-trichlorodiphenyl can be detected by using the surface enhanced raman scattering effect of the top silver-cladding gold nanorod array.

The invention discloses a method for preparing gold nano-rods. The method includes the steps: adding chloroauric acid solution and optional gold seed generating regulating agents into CTAB (cetyl trimethyl ammonium bromide) solution, adding silver nitrate solution, weak reducing agents and strong reducing agents into the CTAB solution and reacting at the constant temperature of 25-40 DEG C for 5-30min to obtain reaction liquid A; and adding silver nitrate solution, optional gold nano-rod growth regulating agent solution and water into the reaction liquid A to obtain reaction liquid B, and continuing reaction to obtain the gold nano-rods. The method is simple in process and operation and fine in reproducibility, the diameter of coverage of the prepared gold nano-rods is as small as 5nm to tens of nanometers, LSPR (localized surface plasmon resonance) peak value coverage in the length direction ranges from 630nm to 1010nm, counts in a TEM (transmission electron microscopy) graph indicate that more than 90% of rod products among obtained gold nano-particle products are high in rod yield, and the ratio of an LSPR peak value to a TSPR (transverse surface plasmon resonance) peak value is not lower than 2 in a UV-Vis (ultraviolet visible) absorption spectrogram. Raw materials used in the method are widely and easily obtained, and production cost is low.

The invention discloses a dual-mode optical coding probe and a preparation method thereof. The probe is in a three-layer core-shell structure, the first-layer core is a gold nano bar, the second-layer shell is silica, the third-layer shell is a cadmium telluride quantum dot, the second-layer shell is wrapped on the outer side of the first-layer core, the third-layer shell covers the outer side ofthe second-layer shell in a sticking mode, and the outer surface of the first-layer core is stuck with Raman molecules which are wrapped by the second-layer shell. The preparation method of the dual-mode optical coding probe comprises the following steps of: 1, preparing an original gold nano bar solution; 2, preparing gold nano bars marked by the Raman molecules; 3, preparing a gold nano bar andsilica metal medium composite nano ball solution; and 4, preparing the dual-mode optical coding probe. The dual-mode optical coding probe has the joint encoding capacity of fluorescence and SERS (Surface Enhanced Raman Scattering), and enhances the optical coding capacity. The preparation method of the dual-mode optical coding probe has a simple process and high repeatability.

The invention discloses a pH-responsive anti-tumor drug carrier material and a preparation and application of the pH-responsive anti-tumor drug carrier material. The preparation method comprises the following steps that (1) gold nanoparticles wrapped by mesoporous silica are prepared, and the gold nanoparticles comprise gold nanospheres or gold nanorods; (2) gold nanoparticles wrapped by carboxylation mesoporous silica are prepared; (3) water-soluble zinc oxide quantum dots are prepared; and (4) the pH-responsive anti-tumor drug carrier material is prepared. According to the pH-responsive anti-tumor drug carrier material and the preparation and application of the pH-responsive anti-tumor drug carrier material, the whole technological process design of the preparation method, the parameter conditions adopted by the reaction steps and the like are optimized, and the existing problems that drug releasing is uncontrollable, the drug loading capacity is low, and side effects are prone to being caused when the gold nanoparticles wrapped by the mesoporous silica serve as a drug carrier can be effectively solved.

The invention discloses a gold nanorod-silicon dioxide core-shell structure nanometer material. The core structure of the core-shell structure nanometer material is a chiral gold nanorod side-by-side assembly with the circular dichroism optical activity. The shell structure of the core-shell structure nanometer material is silicon dioxide. The core of the chiral gold nanorod side-by-side assembly is coated with the silicon dioxide shell layer, so that the nanometer material with the circular dichroism optical activity is obtained. According to the nanometer material, the side-by-side assembly structure is stable, the solution state is stable, and the circular dichroism optical activity is obviously improved, wherein the circular dichroism optical activity is improved by 4-6 times compared with a pure chiral gold nanorod, and the stability is kept as long as 6 months. A preparation method of the gold nanorod-silicon dioxide core-shell structure nanometer material is simple and low in cost. The obtained chiral gold nanorod side-by-side assembly-silicon dioxide core-shell structure nanometer material has various compositions and properties, is wide in application range and has the large development space.

The invention relates to a saturable absorber based on a plasmon axial vibration mode and belongs to the technical field of pulse laser. The invention particularly relates to a novel saturable absorber which consists of gold nanorods and film forming agent and can be used for mode-locked fiber lasers. The saturable absorber based on the gold nanorods can be prepared by adopting the method which comprises the steps of mixing the gold nanorod water dispersion and film forming agent water solution according to a molar ratio of (8-133):1, and performing ultrasonic dispersion for 2-5hours; and finally spin-coating the mixed solution on surfaces of flat silicon wafers and naturally drying the silicon wafers in a vacuum closed container till films are formed. The output of pulse laser at a near-infrared band is realized by using the unique longitudinal surface plasma resonance absorption characteristic of the gold nanorods. The saturable absorber based on the plasmon axial vibration mode enables the types of saturable absorbers to be expanded and can be widely used for mode locking of lasers at a visible-near infrared band.

The invention relates to a platinum-based alloy structured nanorod simulation enzyme solution and application thereof in ELISA (Enzyme-Linked Immunosorbent Assay). The platinum-based alloy structured nanorod simulation enzyme solution contains a gold kernel-platinum-based alloy-antibody or antigen composite structure which consists of a cylindrical gold nanorod kernel, an island-shaped platinum-based alloy structure and an antibody or antigen, wherein the outside surface of the cylindrical gold nanorod kernel is coated with the island-shaped platinum-based alloy structure, and the outside surface of the cylindrical gold nanorod kernel is coated with the antibody or antigen. When applied to the ELISA, the platinum-based alloy structured nanorod simulation enzyme solution disclosed by the invention has the advantages that the response range is wide in detection on the antibody or antigen, the sensitivity is high, and the detection limit can reach the level of pg / mL. Particularly, when a gold kernel / copper-platinum alloy shell nanorod is used, the separation of binding sites and the catalysis sites can be realized, and coexisting protein has not obvious influence on the catalytic activity of nanostructures.

The invention provides a method for preparing surface enhanced Raman substrate, and relates to Raman spectrums. The method comprises the steps that a seed crystal growing method is used for synthetizing a gold nanorod solution, wherein gold nano seeds are synthetized first, then a growing solution is added, silver nitrate is used for adjusting the slenderness ratio of gold nanorods until reaction is completed, the needed gold nanorod solution is obtained, then centrifuging is conducted to remove supernatant liquor, signal molecules are added to be connected to a gold surface, then a polyethylene glycol dressing agent is added to induce the gold nanorods to conduct self-assembling, then a processed nano particle solution is stirred, a TEOS methanol solution is added for reacting, silicon dioxide layers are wrapped on the surfaces of the self-assembled gold nanorods, and the surface enhanced Raman substrate is obtained. Detecting can be directly conducted in the solution, due to self-assembling and the coupling effect of plasma on the surfaces of adjacent nano particles, the electromagnetic field between the nano particles is obviously enhanced, the Raman signals at the position are amplified in a quantity level mode, accordingly, the detecting sensitivity of surface enhanced Raman is greatly improved, and the stability is high.

The invention discloses a method for rapidly preparing a gold nanorod, which belongs to the technical field of a nano material, and solves the problem that the existing gold nanorod seed induction growth method is long in consumed time. The method is characterized in that cationic surface active agent chloride quaternary ammonium salt is used as a protective agent, gold seeds which are obtained by reducing sodium borohydride through the reducing agent sodium oleate, ascorbic acid are added into a chloroauric acid solution under the assistant effect of ions and silver ions to obtain gold nanorods. According to the method, the synthesis of the gold nanorods can be completed in 15 minutes, so that not only can the synthesis time of the gold nanorods be greatly shortened and a material base be provided for the real application of the gold nanorods, but also the yield of the gold nanorods is high, and fewer spherical particles exist.

The invention discloses a method for preparing gold nanorods and belongs to the field of nano material preparing. The method includes the following steps that firstly, a mixed solution of quaternary ammonium bromide, oleate, chloroauric acid hydrated, silver nitrate, an acid solution and ascorbic acid is prepared, and a growth solution is obtained; secondly, in a cetyl trimethyl ammonium bromide surface modifying solution, strong reductant sodium borohydride is used for restoring the chloroauric acid hydrated to obtain a gold seed solution; thirdly, the gold seed solution is injected into the growth solution for reaction for 3-15 hours, and the gold nanorods are obtained. The method for preparing the gold nanorods has the advantages that the requirement for equipment is low, the method is easy to operate, conditions are gentle, reproducibility is high, and large-batch preparing can be conducted; the obtained gold nanorods are high in yield which can reach 99 percent or higher, consistency is high, the variable coefficient of length and the variable coefficient of width are within 10 percent, the length-diameter ratio can be regulated in the large range of 1.5-8, and a solid foundation is laid for practical use of the gold nanorods.

The invention relates to a method for performing supersensitive detection on fungaltoxin of a DTNB mark-based gold@silver core-shell nanorod, and belongs to the technical fields of food safety, environment monitoring and the like. The gold nanorod is prepared by a seed growing method, 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) is marked on the gold nanorod, meanwhile, the gold nanorod is coated with a silver shell layer to prepare a gold@DTNB@silver surface Raman enhanced substrate, a superparamagnetic magnetic material chitosan ferroferric oxide is prepared, a fungaltoxin aptamer complementary chain is coupled on the prepared Raman enhanced substrate, a fungaltoxin aptamer chain is coupled on the magnetic material, an enhancer and the magnetic material are combined and a Raman signal of a system is strongest when the fungaltoxin does not exist in the detection system, and the aptamer modified magnetic material is specifically and preferentially combined with the fungaltoxin and the Raman signal of the system is changed after an external magnetic field is separated when the fungaltoxin exists, so that the aim of quantitatively detecting the fungaltoxin is fulfilled.

A nanoagent includes at least one nanocomposite. The nanocomposite includes at least one gold nanorod, a silver layer coated on an outer surface of the gold nanorod, a Raman reporter molecule layer coated on the silver layer, a pegylated layer coated on the Raman reporter molecule layer, an active layer conjugated to the pegylated layer. the active layer includes at least one of a targeting molecule configured to bind to a target of interest, and a functional molecule configured to interact with the target of interest. The silver layer has silver nanoparticles. The Raman reporter molecule layer has Raman reporter molecules that are detectable by surface enhanced Raman spectroscopy (SERS). The pegylated layer has at least one of thiolated polyethylene glycol (HS-PEG), thiolated polyethylene glycol acid (HS-PEG-COOH) and HS-PEG-NHx.

The invention provides a composite gold nanorod carrier having photo-thermal / photodynamic therapy treatment performance and a preparation method thereof. The composite gold nanorod carrier uses a gold nanorod as a core and uses silicon dioxide as a shell layer, a near infrared fluorescent dye for photodynamic therapy is modified on the outer surface of the shell layer. The preparation method comprises the steps that a chloroauric acid solution and a cetyl trimethyl ammonium bromide solution are mixed, a sodium borohydride ice water mixed solution is added to obtain a nano gold seed solution A; a silver nitrate solution and a chloroauric acid solution are added to a binary surface active agent solution, a hydrochloric acid is added after reaction to obtain a solution B, then ascorbic acid and the solution A are added, and a gold nanorod is obtained after reaction; a tetraethoxysilane methanol solution and an aminopropyl trimethoxy silane methanol solution are added to a gold nanorod solution to obtain composite nanoparticles; 1-(3-dimethyl amino propyl)-3-ethyl carbodiimide hydrochloride is added to the near infrared fluorescent dye, then N-hydroxyl succinimide is added to obtain a solution C; the composite nanoparticle solution is mixed with the solution C to obtain a product.

The invention discloses a drug carrier, a preparation method, a pharmaceutical composition made from the drug carrier, and applications of the drug carrier and the pharmaceutical composition. The mesoporous silica-coated gold nanorod (Au@SiO2) carrier is characterized in that: gold nanorods are coated with a layer of mesoporous silica. According to the preparation method, the Au@SiO2 carrier is prepared by taking hexadecyl trimethyl ammonium bromide-coated gold nanorods as raw materials via hydrolytic polymerization with orthosilicate ester, can be used for coating a plurality of drugs and probe molecules, and delivering the drugs and the probe molecules to parts of diseases so as to realize targeting therapy. After absorption of near-infrared laser, the Au@SiO2 carrier, or drug-loaded Au@SiO2 nanoparticles are capable of emitting fluorescence and transforming a part of light energy into heat energy, so that the Au@SiO2 carrier and the drug-loaded Au@SiO2 nanoparticles can be used for bioimaging, light-dependent control drug release (chemotherapy), and thermotherapy. The preparation method of the Au@SiO2 carrier is simple; the Au@SiO2 carrier can be used for a plurality of diagnosis and treatment methods, and the plurality of diagnosis and treatment methods can be combined, so that it is beneficial for avoiding defects of a single diagnosis and treatment method, and the drug carrier and the pharmaceutical composition are especially suitable for tumor diagnosis and treatment with complex requirements.

The invention discloses a preparation method of a gold nanorod array. The method includes the steps that a gold nanorod aqueous solution modified by cetyl trimethyl ammonium bromide (CATB) is prepared; the final concentration of gold nanorods and the final concentration of the CATB are adjusted to be 0.2-0.6 nmol / L and 0.5-2 mmol / l respectively; sulfhydryl compounds are added and stirred, and a mixed solution can be obtained; the final concentration of the sulfhydryl compounds is 0.01-0.2 mmol / L; after being concentrated, the mixed solution is dripped to a hydrophilic planar substrate; and the gold nanorod array is obtained after natural drying. The preparation method of the gold nanorod array is easy to operate and mild in condition. The prepared gold nanorod array is regular in structure and at the macroscopic millimeter level; and evenly-distributed SERS hot spots are arranged on the surface of the gold nanorod array, and therefore the gold nanorod array can serve as an SERS enhanced substrate easily. The invention further provides the gold nanorod array and application thereof.

The invention provides a preparation method and an application of a graphene oxide-gold nano-rod composite nano-material. The method comprises the following steps: 1, sequentially adding silver ions, ascorbic acid (VC) and an auric chloride acid solution to synthesize gold nano-rods with colloidal gold nanoparticles as nucleation sites; 2, selecting a monolayer graphene oxide solution, further processing by using NaOH and sodium monochloroacetate, neutralizing an alkali residual after a reaction by using diluted hydrochloric acid, and modifying by an active carboxyl group to prepare sulfhydrylated graphene oxide (GO-SH); and 3, taking the gold nano-rods (GNRs) prepared in step 1 and the GO-SH prepared in step 2, stirring for full mixing, standing, centrifuging, and dispersing the obtained material in deionized water. The method has the advantages of simplicity, strong operationality and good reappearance, and the prepared nano-material has very good mono-dispersion and stability in an aqueous solution, and provides powerful safeguard for application in biological detection, sensing and chemical analysis.

The invention provides a nano rod and a preparation method and application thereof. The preparation method includes: in the presence of solvent, allowing soluble platinum salt, soluble copper salt and reducing agent to be in contact with a gold nano rod, wherein for each mol of gold atom, 0.1-0.5mol of soluble platinum salt is used, 0.01-3mol of soluble copper salt is used, and 2-20mol of reducing agent is used. The nano rod prepared by the method has CuPt alloy nano island structures, selectivity on substrate catalyzing and high catalyzing efficiency are achieved, the catalytic activity, on an OPD, TMB+H2O2 system and H2O2, of the nano rod is evidently higher than that of the gold nano rod having only deposited platinum on the surface, and the nano rod prepared by the method is applicable to the fields of chemical and biological catalysis. In addition, the CuPt alloy nano island structures gather to two ends of the gold nano bar when the use amount of the soluble copper salt and the soluble platinum salt is increased, and accordingly nano rods of different morphologies and components can be obtained by the method.

The invention provides a method for manufacturing a plasma chiral ligand sensor for mercury ions, and belongs to the technical field of analytical chemistry and the technical field of food safety. The method mainly comprises the following steps of: (1) synthesizing gold nanorods; (2) performing directional nucleic acid functionalization on surfaces of the gold nanorods; (3) constructing the plasma chiral ligand sensor by using the gold nanorods after the surfaces of the gold nanorods are subjected to the directional nucleic acid functionalization; (4) representing the plasma chiral ligand sensor; (5) measuring the specificity of the plasma chiral ligand sensor; and (6) measuring an actual sample of the plasma chiral ligand sensor. Along with increase of Hg2+ concentration, the number of the gold nanorods in a gold nanorods side surface assembly body is increased, and the Mohr ellipticity of the assembly body is also increased. The linear relation between the Mohr ellipticity and the concentration of the mercury ions is set up, and the plasma chiral ligand sensor which is simple and sensitive and is high in specificity is manufactured and is used for detecting the concentration of the mercury ions.

The invention discloses a simply synthesized gold nanorod-CdS-gold nanoparticle composite photocatalyst with visible and near-infrared light response. The photocatalyst is used for organic synthesis in a water phase and prepared from two Au nanostructures (gold nanorods and gold nanoparticles) different in morphology and one-dimensional semiconductor CdS nanowires by a simple electrostatic self-assembly method. For the first time, the simply synthesized gold nanorod-CdS-gold nanoparticle composite photocatalyst is used for photocatalysis in the water phase to realize selective reduction for substitution of nitrobenzene. The photocatalyst has high catalysis efficiency and high selectivity. In addition, the preparation process is simple, selective reduction for substitution of the nitrobenzene is realized by taking the visible and near-infrared light as driving energy, water as a solvent and nitrogen as a shielding gas, and sustainable development of environment and energy is benefited.

The invention relates to an electrochemiluminescence immunosensor for detecting beta-amyloid protein and construction of the electrochemiluminescence immunosensor. The sensor comprises a magnetic glassy carbon electrode and Fe3O4-expoxy, a mesoporous carbon @Ru(bpy)32+ / beta-amyloid protein antibody composite nanometer composite material, beta-amyloid protein to be detected with different concentrations and a gold nanorod-beta- beta-amyloid protein aptamer nanometer composite material, wherein the Fe3O4-expoxy, the mesoporous carbon @Ru(bpy)32+ / beta-amyloid protein antibody composite nanometer composite material, beta-amyloid protein to be detected with different concentrations and the gold nanorod-beta- beta-amyloid protein aptamer nanometer composite material are synthesized on the surface of the magnetic glassy carbon electrode in sequence. Compared with the prior art, the electrochemiluminescence immunosensor has the advantages that the constructing method is simple, detection on Alzheimer's disease markers (beta-amyloid protein and the like) is high in speed, high in sensitivity, high in specificity, low in detection limit, wide in detection range and the like, and the application of the electrochemiluminescence immunosensor provides a new thought for early diagnosis of the Alzheimer's disease and detection of markers of other diseases.

The invention discloses a gold nanorod immunoprobe applied to various mediums, and a preparation method and application thereof. The gold nanorod immunoprobe is a product obtained by performing the preparation of gold nano seeds, the growth and concentration of a gold nanorod, antibody modification and sealing. The immunoprobe is subjected to specific recognition in various mediums, such as buffer solution, serum, blood plasma or urine, saliva and the like, and is finally qualitatively and quantitatively analyzed by reading the offset of longitudinal plasma absorption peaks of the gold nanorod. When used for detecting corresponding antigens, the immunoprobe has the advantages of simple operation, high detection sensitivity, high specificity, fewer required instruments and devices, and the like. Moreover, as nano composites are well adapted to external mediums by the special modification mode of the immunoprobe, the immunoprobe also has an expanded application range and is possibly popularized to clinical application.

The invention discloses a silver-gold porous nanorod array, a preparation method and a purpose of the silver-gold porous nanorod array. The array comprises porous gold nanorods coated with 3-15nm silver films and arranged on a methyl methacrylate substrate coated with a gold film, wherein the nanorods are 150-250nm in length, and 50-70nm in diameter; and holes are 5-20nm in diameter. The method comprises the steps of placing an aluminum oxide template coated with a gold film in a mixed electrolytic solution for electro-deposition, obtaining an aluminum oxide template coated with the gold film and provided with a gold-silver alloy nanorod array deposited in holes, placing the aluminum oxide template in a nitric acid solution for reaction to obtain an aluminum oxide template coated with the gold film and provided with a porous gold nanorod array in holes, coating the other side of the gold film with liquid methyl methacrylate, solidifying, then sequentially placing the aluminum oxide template in a silver nitrate electrolytic solution for the electro-deposition and aqueous alkali for removing the aluminum oxide template, or sequentially placing the aluminum oxide template in aqueous alkali for removing the aluminum oxide template, coating a silver film by an ion sputtering method, and obtaining the objective product. The array can serve as an SERS (Surface Enhanced Raman Scattering) activity substrate for detecting trace organic matters.

The invention discloses a small-size gold nanorod and a preparation method and applications thereof. The growth reaction is adjusted by utilization of cationic-anionic surfactants as a mixed growth template, and synthesis of the small-size gold nanorod with the diameter of 5-9 nm is achieved through the non-gold-seed one-step method. The diameter and the length-diameter ratio of the small-size gold nanorod are adjusted through changes of the mole ratio of the chloroauric acid to the cationic-anionic surfactants to the silver nitrate. Operation is simple, consumed time is short, repeatability is good, and the small-size gold nanorod good in monodispersity and uniform in size can be obtained with no ultrasound in the preparation process. The diameter of the small-size gold nanorod is 5-9 nm, the size is small, and the ratio of the absorption value of a longitudinal surface plasma resonance absorption peak to the absorption value of a transverse surface plasma resonance absorption peak of the small-size gold nanorod is 2.5-5.5. The prepared small-size gold nanorod can be applied to the fields of preparation of photo-thermal treatment drugs, optical mark drugs, sensors and the like.

The invention discloses a preparation method of a sandwich type lung cancer tumor marker immune sensor and the application of the sandwich type lung cancer tumor marker immune sensor to lung cancer tumor marker detection, belonging to the fields of novel nanometer functional materials and a biosensor. According to the preparation method, a precious metal nanometer material, namely a gold @ silver core-shell nanorod is taken as biosimulation enzyme, a second antibody is taken as a marker after being incubated, and nanometer materials including a gold nanorod and reducing graphene are taken as electrode modifiers, so that the sandwich type electrochemical immune sensor which is used for detecting the lung cancer tumor marker and is low in cost, high in sensitivity, good in specificity, rapid in detection and simple in preparation can be prepared.

A nanoagent for surface enhanced Raman spectroscopy (SERS) detection of a target of interest, includes one or more nanocomposites. The target of interest may include at least one tumor cell or at least one pathogen. Each nanocomposite includes at least one gold nanorod, a silver layer coated on a surface of the gold nanorod and having silver nanoparticles, a Raman reporter molecule layer assembled on the silver layer and having Raman reporter molecules, a pegylated layer coated on the Raman reporter molecule layer, and an antibody layer conjugated to the pegylated layer. The different nanocomposites of the nanoagent can be represented by different colors when SERS images are collected upon the nanoagent.

The invention provides a modified gold nanorod. The surface of the modified gold nanorod is wrapped by gold nanocluster which is wrapped by bovine serum protein (BSA), so as to form a novel nanometer composition. The composition is exposed under the irradiation of near infrared light and used for exploring the photothermal performance and photothermal stability. After irradiation of near infrared light for many times, the optical property and shape of the gold nanocluster-gold nanorod composition have no obvious change, and the temperature is obviously increased. As a comparison, the identical gold nanorod and the bovine serum protein wrapped gold nanorod are sequentially used as stability control experiments. In addition, the photothermal performance and the glue stability of the gold nanocluster-gold nanorod composition have extremely good reproducibility. Therefore, the nano composition capable of improving the photothermal property and the photothermal stability is very suitable for further biological applications, such as remote sensing, bioimaging and photothermal therapy.

The invention discloses an ordered porous gold nanorod array and a preparation method and application thereof. The array is that the porous gold nanorod array is arranged on a methyl methacrylate substrate coated with a gold film. Length of porous gold nanorods on the gold film is 150-250nm, diameter of the nanorods is 50-70nm, and diameter of upper holes of the nanorods is 5-20nm. The method includes: an alumina template with one surface plated with a gold film is placed in mixed electrolyte to perform electrolytic deposition, the alumina template with the gold film plated on one surface and gold-silver alloy nanorods arrays deposited in holes is obtained, the alumina template is placed in a salpeter solution to react to obtain the alumina template with the gold film plated on one surface and porous gold nanorod arrays placed in the holes, liquid-state methyl methacrylate is coated on the other surface of the gold film of the alumina template with the gold film plated on one surface and porous gold nanorod arrays placed in the holes, the alumina template with the gold film plated on one surface and porous gold nanorod arrays placed in the holes is placed in an alkali solution to remove the alumina template after the liquid-state methyl methacrylate is solidified, and a target product is obtained. The ordered porous gold nanorod array can be used as a surface enhanced Raman scattering (SERS) active substrate to detect trace rhodamine.

The invention provides a preparing method and an application of self-assembly structures of gold and silver core-shell particles and gold nanometer rods and belongs to the field of analytic chemistry. The preparing method comprises the synthesis of the gold and silver core-shell nanometer particles, the synthesis of the gold nanometer rods, the nucleic acid modification of the gold and silver core-shell nanometer particles, the fluorochrome and aptamer modification of the gold nanometer rods, the self-assembly of dimers of the gold and silver core-shell particles and the gold nanometer rods, Raman beacon modification and the bimodal quantitative detection of dopamine. The self-assembly structures integrate SERS (surface-enhanced Raman scattering) with fluorescence signals, and the dimers of the gold and silver core-shell particles and the gold nanometer rods, combining the SERS and fluorescence signals are formed. With the variation of distances between the gold nanometer rods and the gold and silver core-shell particles and the regulation of the surface plasma resonance properties of the assembly structures, the dopamine can be accurately quantified by monitoring the variation of the strength of the SERS and fluorescence signals.