565 results about "Succinimide" patented technology

The invention discloses a bacteria surface modification method based on atom transfer radical polymerization reaction mediation, obtained bacteria and application. The modification method comprises the following steps: carrying out acryloylation on the surfaces of the bacteria by using N-hydroxysuccinimide acrylate, and introducing double bonds on the surfaces of the bacteria to obtain acryloylated bacteria; the method comprises the following steps: by taking acrylamide, N-(3-aminopropyl) methacrylamide hydrochloride and N, N '-methylene bisacrylamide as monomers, carrying out polymerization reaction on the surfaces of acrylated bacteria in the presence of an initiator and a catalyst; the mass ratio of the acrylamide to the N-(3-aminopropyl) methacrylamide hydrochloride to the N, N '-methylene bisacrylamide is (11.9 to 17.5) to (0.125 to 1) to (0.1 to 0.5). According to the method, bacteria can be camouflaged to reduce immunogenicity and pathogenicity of the bacteria, the safety of bacteria treatment is improved under the condition that the viability and multiplication capacity of the bacteria are not affected, the resistance of the bacteria to environmental threats is enhanced, and the bacteria are endowed with no exogenous functions.

The invention relates to a preparation method of a high-hardness and high-sulfate-radical water quality scale inhibitor, and belongs to the technical field of water treatment.The preparation method comprises the steps that sodium carboxymethyl cellulose-sulfur quantum dots are prepared by using carboxymethyl cellulose as a stabilizer and then subjected to a copolymerization reaction with 2-acrylamido-2-methylpropanesulfonic acid and itaconic acid, and the high-hardness and high-sulfate-radical water quality scale inhibitor is obtained. The preparation method comprises the following steps: carrying out a reaction on sodium carboxymethyl cellulose and sulfur quantum dots to obtain modified sodium carboxymethyl cellulose-sulfur quantum dots, and carrying out a reaction on polysuccinimide, 4-amino phthalic acid and 2-aminoethanesulfonic acid to obtain a polyaspartic acid derivative; the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative are mixed to serve as the scale inhibitor, the scale inhibition rate of the scale inhibitor is remarkably increased through the synergistic effect of the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative, and meanwhile, due to the fact that a large number of new hydrogen bonds are generated between the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative, the scale inhibition rate of the scale inhibitor is increased. The stability of a polymer structure is improved, so that the temperature resistance of the scale inhibitor is improved. The scale inhibitor is green and environment-friendly, and has excellent scale inhibition efficiency on high-hardness and high-sulfate-radical water.

The invention discloses a polymer drug-loaded particle for targeted adhesion to an intestinal inflammation area and a preparation method and application thereof.The method includes the steps that degradable polymer drug-loaded particles are subjected to surface modification through polycations and then added into a monomer solution of an adhesion layer, particle surface in-situ polymerization is initiated through ultraviolet irradiation, and a PAN adhesion layer is formed; the adhesion layer modified polymer drug-loaded particles are obtained. Wherein the polycation is used as a middle layer, so that the PAN adhesion layer can be successfully modified. Carboxyl in the adhesion layer enables the particles to present negative charges, meanwhile, succinimide ester can react with amino naturally existing on the surface of inflammatory intestinal tissue to generate an amide covalent bond, and finally targeted adhesion of the intestinal inflammation part is achieved. According to the adhesion strategy, the PAN can be effectively applied to the micro-nano-scale drug-loaded particles so as to enhance the adhesion of the micro-nano-scale drug-loaded particles in an intestinal tract damaged area, and a brand new thought is provided for treating inflammatory intestinal tracts.

The invention discloses a cold-rolled silicon steel thin strip and a processing method thereof, and relates to the technical field of silicon steel strips. According to the method, the silicon steel plate is cold-rolled into the ultrathin silicon steel strip, then the ultrathin silicon steel strip is subjected to activating treatment through the activating solution and then coated with the anti-corrosion coating, and the prepared ultrathin silicon steel strip has good corrosion resistance. According to the invention, phytic acid and modified polyaspartic acid are added into an activating solution. According to the iron-based organic framework material, polysuccinimide is used as a raw material, dopamine is added for grafting modification, a polymer which takes polyaspartic acid as a framework and is grafted with dopamine is obtained, the dopamine has a catechol group, and the chelating property of the polyaspartic acid to metal ions on the iron-based organic framework material is further enhanced. And meanwhile, phytic acid is added into the activating solution, the phytic acid and the modified polyaspartic acid can form a nano-porous structure, loading of subsequent modified silica sol is facilitated, and therefore the corrosion resistance of the ultra-thin silicon steel strip is further enhanced.

The invention relates to an antibody endocytosis efficiency detection method based on fluorescence resonance energy transfer, and belongs to the technical field of antibody endocytosis detection. The method comprises the following steps: coupling succinimide ester with an amino-containing compound to obtain a quenching agent, incubating an antibody to be detected and a target cell together, adding a fluorescence-labeled secondary antibody to carry out endocytosis reaction, removing an extracellular fluorescence signal by using the quenching agent after the reaction is finished, and detecting an intracellular fluorescence signal to obtain the endocytosis percentage of the antibody to be detected. The detection method provided by the invention is based on a fluorescence resonance energy transfer principle, an intracellular signal direct detection method in the prior art is optimized, and an extracellular fluorescence signal is removed, so that a detection system has higher sensitivity. The quenching agent provided by the invention can also be combined with an acid eluent in the prior art to further reduce an extracellular signal background value and improve the sensitivity of a detection system, can be applied to high-throughput detection of a to-be-detected antibody, is high in sensitivity and low in detection cost, and has a good application prospect.

A lubricating composition includes an oil of lubricating viscosity, a phenolic antioxidant, a succinimide dispersant which is at least substantially free of boron, a detergent comprising an alkaline earth metal salicylate and a sulfurized organic compound.

The invention discloses injectable hydrogel with anti-inflammatory and drainage functions for promoting anal fistula healing and a preparation method of the injectable hydrogel. The preparation method comprises the following steps: adding acrylic acid-N-succinimide ester and acrylated beta-cyclodextrin into a gelatin solution; then adding a carbon nanotube grafted with poly (vinyl benzyl trimethyl ammonium chloride), stirring and carrying out ultrasonic treatment; adding ammonium persulfate, stirring and performing ultrasonic treatment to obtain a mixture A; and heating the mixture A, cooling, washing and mechanically crushing to obtain the GAA coated CNT-g-PVBTMA hydrogel. The hydrogel is novel quaternized molecular brush grafted injectable hydrogel, has excellent swelling resistance and a broken porous structure, can effectively fill a fistula, and is beneficial to discharge of exudate; the hydrogel can also capture inflammatory cytokines of the fistula through electrostatic interaction, and shows excellent anti-inflammatory characteristics.

The invention belongs to the technical field of biological materials and ultrasonic printing, and particularly discloses a biological adhesive for ultrasonic printing and preparation and application thereof. The biological adhesive comprises a heat-sensitive main body material and a chemical adhesion component, the heat-sensitive main body material comprises hydroxybutyl chitosan, and the chemical adhesion component comprises polyacrylic acid-N-hydroxysuccinimide ester. The bioadhesive has excellent ultrasonic responsiveness, formability and bioadhesion performance, directional deposition, in-situ solidification and tissue surface adhesion can be achieved in a non-contact and accurately-controlled ultrasonic printing system, and the bioadhesive is suitable for various biomedical scenes such as tissue repair, minimally invasive medicine, biological device construction and drug delivery interface printing.

The invention belongs to the field of drug-loaded nanoparticles, and provides a targeted drug-loaded nanoparticle with multiple responsiveness and a preparation method thereof. The targeted drug-loaded nano-particle with multiple responsiveness is prepared from poly (N-isopropylacrylamide-co-acrylic acid-co-N-acryloyloxy succinimide) nanogel with temperature and pH response characteristics, an anti-tumor drug and a folic acid layer capable of specifically targeting a folic acid receptor of a tumor cell, wherein the temperature and pH response characteristics are realized by the poly (N-isopropylacrylamide-co-acrylic acid-co-N-acryloyloxy succinimide) nanogel; the anti-tumor drug is loaded in the polymer network structure of the nanogel to form drug-loaded nanoparticles, the folic acid layer is composed of aminated folic acid molecules, the folic acid layer coats the drug-loaded nanoparticles, and amino groups of the aminated folic acid molecules are grafted on the surfaces of the drug-loaded nanoparticles. According to the invention, the preparation method of the existing chemotherapy nano-drug carrier can be simplified, the controllability of the preparation process is improved, the biocompatibility of the existing chemotherapy nano-drug carrier is improved, and the targeting property of the existing chemotherapy nano-drug carrier is enhanced.

The invention relates to the technical field of new material application, and discloses a magnetic bead material for enriching a sulfydryl peptide fragment and a preparation method thereof.The magnetic bead material prepared from micron carboxyl magnetic beads, dodecane dicarboxylic acid dihydrazide and SPSP has a long chemical reaction connecting arm, so that a reaction group has the better freedom degree, the reaction with sulfydryl is better facilitated, and the magnetic bead material can be used for enriching the sulfydryl peptide fragment. The enrichment efficiency is relatively high. When the magnetic bead material is prepared, micron carboxyl magnetic beads and dodecane dicarboxylic acid dihydrazide are based on hydrazine group and carboxyl condensation, the condensation reaction is more selective and more stable, the reaction is carried out under a milder condition, the reaction product is stable, and the reaction efficiency is higher at a lower reaction temperature. Dodecane dicarboxylic acid dihydrazide and SPSP are based on a reaction of N-succinimide and hydrazine, N-succinimide has high specificity to amino-containing molecules, side reactions are reduced, the reaction is generally carried out at room temperature, strong acid and alkali conditions are not needed, and protection of other sensitive groups is facilitated.

The invention belongs to the technical field of bone repair hydrogel, and discloses a hydrogel band-aid for bone repair and a preparation method and application thereof, and the preparation method comprises the following steps: mixing a polyethylene glycol succinimide succinate solution with an alendronate solution, dialyzing, and freeze-drying to obtain alendronate modified polyethylene glycol succinimide succinate; and uniformly mixing alendronate modified polyethylene glycol succinimide succinate, polyphenol, metal ions and water in proportion, carrying out solid-liquid separation, and drying the solid to obtain the hydrogel. The preparation process provided by the invention has the advantages of simplicity, convenience and rapidness, not only simplifies the production process, but also enhances the bone regeneration, angiogenesis and immunoregulation capabilities.

The invention discloses a hydrogel for gastric perforation repair, which belongs to the field of bioadhesives. The technical scheme is as follows: a hydrogel for gastric perforation repair is generated by chemical reaction of compound A and compound B; compound A is a polyethylene glycol derivative A with a terminal active group α; the terminal active group α is succinimidyl succinate; compound B is a polyethylene glycol derivative B with a terminal active group β; the terminal active group β is an amino group; when compound A reacts with compound B, the terminal active group α and the terminal active group β chemically react to form an amide bond. The beneficial effects of the invention are as follows: the hydrogel for gastric perforation repair provided by the invention has a fast gelation speed, can be gelled in situ, is easy to use, has strong adhesion to gastric tissue, has a low swelling rate in simulated gastric fluid and PBS solution, has a strong ability to resist simulated gastric fluid corrosion, has good compression resistance and anti-peristalsis performance, has good biocompatibility, and can be safely degraded.

The invention discloses a succinimide leaching agent suitable for co-production of light calcium carbonate through CO2 indirect mineralization of alkaline solid waste. The succinimide leaching agent is prepared from a main leaching agent succinimide and a solvent water; the concentration of the main leaching agent succinimide in the succinimide leaching agent is 5-25 wt%. The succinimide leaching agent suitable for CO2 indirect mineralization of alkaline solid waste is simple in composition, high in calcium ion leaching efficiency, high in selectivity, low in leaching agent viscosity and capable of being recycled, and loss and cost of the leaching agent are reduced; in the mineralization stage, CO2 is promoted to be dissolved from a gas phase to a liquid phase, so that the reaction rate of the mineralization reaction is favorably improved, the production efficiency of calcium carbonate is improved, and the process cost is reduced.

The invention relates to the technical field of medical materials, in particular to a tissue-adhesion-resistant polypropylene biomedical monofilament and a preparation method and application thereof, and the tissue-adhesion-resistant polypropylene biomedical monofilament is composed of a polypropylene monofilament and a tissue-adhesion-resistant functional polymer coating; the tissue adhesion resistant functional polymer coating is formed by carrying out free radical copolymerization on N-acryloyloxy succinimide and a zwitterionic monomer. According to the invention, the polymer with the anti-tissue adhesion function is fixed to the surface of the polypropylene monofilament, and chemical bonding is formed on the surface of the monofilament, so that the problem of coating shedding is solved, and the long-term anti-tissue adhesion effect is maintained. After the monofilaments are woven into the patch, the biomedical patch capable of inhibiting tissue adhesion and inflammatory reaction can be obtained without secondary treatment. Compared with the existing patch modification technology, the method is more suitable for industrialization.

The invention belongs to the technical field of immunochemiluminescence, and relates to a PAMAM-mediated labeled small molecule compound and a synthesis method and application thereof.The PAMAM-mediated labeled small molecule compound is composed of small molecules, PAMAM and a marker, and the small molecules are provided with active groups or provided with derivative groups capable of reacting with amino under certain activation conditions to form covalent binding; the marker is provided with a succinimide group. The difference between a coupling product and a small molecular structure is reduced to the maximum extent through PAMAM mediation, the PAMAM dosage is extremely small and stable, compared with PEG, the molecular weight is more uniform, non-specific binding caused by hydrophobic interaction can be greatly reduced in immunoreaction, and good signals and separation can be obtained without purification of the product.

The invention discloses a high-performance clean type anti-wear hydraulic oil complexing agent which is prepared from the following substances in parts by mass: 30 to 60 parts of an antioxidant, 20 to 40 parts of an anti-wear agent, 2 to 5 parts of boronized polyisobutylene bis-succinimide, 2 to 7 parts of thiocarbamate, 4 to 10 parts of dodecenylsuccinic acid and 0.2 to 2 parts of graphene nanosheets. The lubricating oil is prepared from the following components in parts by weight: 0.5-1.5 parts of a metal deactivator, 0-2 parts of DISUPER S24 and 30-50 parts of base oil. The preparation method comprises the following steps: S21, heating the base oil, adding the boronized polyisobutylene bis-succinimide and the thiocarbamate, and stirring; s22, sequentially adding an antioxidant, an anti-wear agent, dodecenylsuccinic acid, DISUPER S24 and a metal deactivator into the solution obtained in the step S11, heating to 80 DEG C, and reacting for 2 hours; and S23, adding graphene nanosheets into the solution obtained in the step S22, and carrying out ultrasonic dispersion for 1 hour to obtain the product.

The invention discloses a non-phosphorus reverse osmosis scale inhibitor and a preparation method thereof, and relates to the technical field of industrial water treatment.The non-phosphorus reverse osmosis scale inhibitor is prepared from, by weight, 10 parts of purified humic acid, 40-60 parts of alkalized acrylic acid-succinimide-hydroxypropyl methacrylate copolymer, 4-8 parts of sodium polyepoxysuccinate and 5-20 parts of water. Compared with the prior art, the scale inhibitor is high in environment-friendly compliance, excellent in scale inhibition performance, scientific and efficient in raw material treatment and high in product scale inhibition rate, meets the environment-friendly emission standard, and is suitable for reverse osmosis membrane scale inhibition treatment in the industries of electric power, chemical engineering, seawater desalination and the like.

The invention discloses a bionic self-crimping material and application in the nerve field, and relates to the technical field of nano materials. The preparation method comprises the following steps: firstly, carrying out ultraviolet photopolymerization on acrylic acid and acrylic acid-N-hydroxysuccinimide, adding chitosan and glutaraldehyde to form hydrogel, and introducing epigallocatechin gallate into a chitosan-acrylic acid network. The material is rapidly and autonomously curled into a tube shape after being in contact with wet nerves, glutaraldehyde is directionally and orderly diffused by adopting a three-channel micro-fluidic chip, and the fineness is improved, so that the matching degree of nerve broken ends with different thicknesses is increased; the surface active groups of the material and the natural component chitosan cooperate to realize suture-free fixation; the plant phenol antioxidant removes free radicals at nerve injury parts; the degradation period is synchronous with natural healing of nerves, and secondary operation for taking out is not needed. The electroneurographic signals using the material are transmitted more smoothly, the target muscle atrophy degree is reduced, and the limb movement function is recovered to be close to the normal level.

The invention belongs to the technical field of water treatment, and particularly discloses a green scale inhibitor for barium sulfate and a preparation method thereof, the scale inhibitor is a product generated by esterification reaction of polysuccinimide and hydroxyethyl methylacrylate under alkaline conditions, and a copolymer formed by graft copolymerization of aconitic acid and sodium lignin sulfonate, wherein the mass ratio of polysuccinimide to hydroxyethyl methylacrylate to aconitic acid to sodium lignin sulfonate is 2: 4: (1-6): (1-4). The scale inhibitor is a composite copolymer formed by polymerizing and grafting a product obtained after esterification of polysuccinimide / hydroxyethyl methylacrylate, aconitic acid and sodium lignin sulfonate, has carboxyl, hydroxyl, amino, sulfonic acid and other groups at the same time, and can generate strong interaction with key ions or sites in the BaSO4 crystal growth process, so that the scale inhibitor can be used for inhibiting the growth of BaSO4 crystals. The normal growth and aggregation of BaSO4 crystals are prevented, so that the formation of BaSO4 scales is effectively inhibited.

The invention discloses a preparation method of deuterated methylamine hydrochloride and a solid-phase continuous flow synthesis system, and relates to the technical field of deuterated synthesis. The preparation method comprises the following steps: adding out-of-phase polykadexide into methyl trifluoromethanesulfonate and 1, 2-dichloroethane for heating reaction, then adding methanol for quenching reaction, washing and drying to obtain an out-of-phase polykadexide type methyl reagent; the method comprises the following steps: adding a heterophase polysulfide-type methyl reagent into a mixed solvent of a polar solvent and heavy water, and carrying out alkali treatment to complete deuteration conversion, so as to obtain a heterophase polysulfide-type deuterated methyl reagent; the preparation method comprises the following steps: dissolving o-phenylsuccinimide in acetonitrile, and adding a heterophase polysulfide deuterium methyl reagent and inorganic alkali to react, so as to obtain N-(1, 1, 1-trideuterium methyl) benzo-succinimide; the preparation method comprises the following steps: hydrolyzing N-(1, 1, 1-trideuterium methyl) benzo-succinimide and hydrochloric acid in an aqueous solvent to obtain deuterated methylamine hydrochloride. The preparation method is simple, the deuterium source is cheap, the atom utilization rate is high, the deuteration rate is high, and the production cost is low.

The invention provides a wound sealing and repairing hydrogel patch and a preparation method thereof. The hydrogel patch is provided with an independent adhesion layer and an adhesion layer and anti-adhesion layer combination. The adhesive layer is prepared from gelatin, N-acryloylated amino acid, acrylic acid-N-succinimide ester, 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone and water through ultraviolet light crosslinking; the anti-adhesion layer is prepared from methacryloyl ethyl sulphobetaine, polyethylene glycol diacrylate, 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone and water through ultraviolet light crosslinking. The hydrogel patch can be used for sealing and repairing wounds of various tissues (such as skin tissues, liver tissues and stomach tissues), the healing time is shortened, the healing effect is improved, the healing time of a skin incision can be shortened from 14 days to 10 days, the hydrogel patch is superior to other treatment modes according to macroscopic photos and related slices, and the hydrogel patch has a good application prospect. And no-injury healing can be realized.

The invention provides an antibody-drug conjugate as well as a preparation method and application thereof. The antibody-drug conjugate comprises an antibody, an effective load with cytotoxicity and a connexon shown in a formula I, and the connexon shown in the formula I is in thioether bond connection with a sulfydryl part reduced by a disulfide chain between antibody chains through a succinimide group; the carbonyl group in the ester group of the linker is connected with the amino group in the effective load. The antibody-drug conjugate can be used for preparing a therapeutic agent for diagnosing, preventing and treating tumor diseases.

The invention belongs to the technical field of environmental protection, and relates to a blocking agent composition, a blocking foaming agent, a preparation method of the blocking foaming agent, soil remediation foam and a remediation method. The blocking agent composition comprises modified protein and a foaming agent; wherein the modified protein is obtained through reaction of natural protein and fatty acid succinimide ester, and based on the total weight of the blocking agent composition, the content of the modified protein is 10-99 wt%, and the content of the foaming agent is 1-90 wt%. The barrier foaming agent at least has the following beneficial effects: the barrier foaming agent provided by the invention has good foaming capacity and stability, and can play a role in barrier and repair for a long time; compared with the reported protein foam composition, the protein compound provided by the invention is simple, easy to obtain and low in cost; the components of the barrier foaming agent are green and degradable, secondary pollution is avoided, and energy conservation and environmental protection are achieved.

The invention discloses a soft polishing antifouling ceramic tile and a preparation method thereof, and belongs to the technical field of ceramic tiles. The soft polishing antifouling ceramic tile sequentially comprises a green body layer, a ground glaze layer, a pattern layer, a protective glaze layer and an antibacterial protein coupling antifouling coating from bottom to top, the coating is formed by combining N-succinimide-3-(2-pyridyl dithio) propionate with antibacterial protein to form a stable amido bond, polyethylene glycol methacrylate is added to introduce a polyethylene glycol chain, and the antibacterial protein coupling antifouling coating is formed by combining the N-succinimide-3-(2-pyridyl dithio) propionate with the antibacterial protein. And finally, an ethanol solution is added, so that the prepared coating can effectively resist various stains. The soft polishing antifouling ceramic tile disclosed by the invention has a good soft light effect and antibacterial and antifouling properties.

The present invention relates to a microneedle-based transdermal delivery patch fabricated from a hydrogel composed of polyvinyl alcohol (PVA), arginine-modified chitosan, and a crosslinker. The arginine-modified chitosan is prepared by reacting a chitosan solution with an arginine solution preactivated with N-hydroxy succinimide and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide at a specific pH. The hydrogel's formulation further comprises a PVA concentration ranging from 10% to 12% (w / v). arginine-modified chitosan and PVA solution are mixed in a 1:2 ratio by volume and are crosslinked using trimethyl orthoformate (TMOF). The patch is further loaded with curcumin, with a concentration of 120μg per patch, demonstrating a 70% release over 48 hours in a controlled manner. This invention represents the first application of PVA crosslinked arginine-modified chitosan hydrogel loaded with curcumin specifically for animal wound healing, providing enhanced wound healing and antibacterial efficacy.

The invention relates to responsive hydrogel loaded with bionic nano-vesicles as well as a preparation method and application of the responsive hydrogel, and belongs to the technical field of medical hydrogel. The method comprises the steps that odontogenic mesenchymal stem cells are stimulated by a chemical vesicle stimulant and then mixed with melatonin mother liquor for extrusion, the bionic nano vesicles are obtained, the chemical vesicle stimulant contains a buffer solution, a dithiothreitol solution and a paraformaldehyde solution, and the buffer solution contains 8.5-9 g / L of sodium chloride, 0.2-0.24 g / L of calcium dichloride and 2.3-2.4 g / L of 4-hydroxyethyl piperazine ethanesulfonic acid; 6-arm-PEG-pp-NH2 coupled with polypeptide capable of being cut by matrix metalloproteinase, six-arm polyethylene glycol succinimide glutarate and the bionic nano-vesicle are prepared into a first precursor solution and a second precursor solution respectively, the first precursor solution and the second precursor solution are mixed and subjected to standing to form gel, and the responsive hydrogel is prepared. According to the invention, the responsive hydrogel capable of obviously enhancing bone regeneration capacity is obtained.

To provide the hydraulic pressure actuating oil composition that has excellent wear resistance and sludge generation suppressing effect even when used under a high pressure condition, and has also excellent brake performance and clutch performance.SOLUTION: The hydraulic oil composition comprises a base oil (A), an antioxidant (B) containing an amine antioxidant (B1) and a phenolic antioxidant (B2), and a succinimide compound (C), wherein the hydraulic oil composition satisfies a following requirement (I) and is used for hydraulic equipment having an operating pressure of 30 MPa or more, and the hydraulic equipment has at least one of a wet brake and a wet clutch, and is selected from construction machines, general industrial machines, and generators. The requirement (I) is a coefficient of static friction (μs) after 1000 cycles of 0.100 to 0.162 which is measured according to a test of No. 2 of SAE described in JCMAS P.047: 2004.SELECTED DRAWING: None

The invention provides injectable chondroitin sulfate-based hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical polymer biomaterials. The hydrogel is prepared by mixing a succinimide functionalized chondroitin sulfate solution and a carboxymethyl chitosan solution and then gelating. The chondroitin sulfate-based hydrogel provided by the invention has good biocompatibility, safety, injectability and stability, and can be used independently or used for filling tissue wounds and promoting rapid healing of the wounds in a drug-loaded manner. Moreover, the chondroitin sulfate-based hydrogel provided by the invention is simple in preparation method, low in cost and wide in potential application range.

The invention provides a preparation method of Fmoc-Lys (Ivdde)-OH, which comprises the following steps: reacting dimedone with isovaleric acid under the action of a condensing agent to generate an intermediate compound, then reacting with N-alpha-tert-butoxycarbonyl-L-lysine under the action of organic alkali to obtain an intermediate, then reacting with acid to obtain an intermediate, and finally, separating and purifying the intermediate to obtain the Fmoc-Lys (Ivdde)-OH. Finally, under the action of inorganic alkali, the Fmoc-Lys (Ivdde)-OH reacts with fluorenylmethoxycarbonyl succinimide, and Fmoc-Lys (Ivdde)-OH is obtained. According to the method provided by the invention, the diketone is taken as a starting raw material, Fmoc-Lys (Ivdde)-OH is synthesized through four steps of reaction including two-step condensation reaction, Boc removal reaction and Fmoc feeding reaction, the process is simplified, the operation is simple, the reaction condition is mild, the industrial production difficulty is reduced, expensive raw materials are not involved, the production cost is favorably controlled, and the obtained product is high in purity and high in yield.

The invention discloses a probe Cvs-B-NHS based on a coumarin derivative as well as a preparation method and application of the probe Cvs-B-NHS. According to the fluorescent probe Cvs-B-NHS of the coumarin derivative, a coumarin mother nucleus and succinimide ester are organically combined, the excellent fluorescence characteristic of coumarin is reserved, efficient protein labeling capacity is achieved, high-sensitivity fluorescence signal output and specific labeling of protein are achieved, and the fluorescent probe Cvs-B-NHS has the advantages of being high in sensitivity and high in sensitivity. And the accuracy and reliability of protein detection and imaging are improved. The invention discloses a TDP-43 protein phase separation labeling method based on a Cvs-B-NHS probe, which provides a new means for researching the action mechanism of a TDP-43 (G335D) mutant in neurodegenerative diseases, and is beneficial to promoting the research progress in the field of neurodegenerative diseases. The application of the Cvs-B-NHS probe in evaluating the polarity change of the microenvironment in the protein liquid-liquid phase separation process in the cytoplasm environment caused by mitochondrial signal disturbance provides a powerful tool for the research of the protein phase separation microenvironment.