322results about How to "High biosecurity" patented technology

The invention provides a tumor cell exosome, an anti-tumor immune drug delivery system and a preparation method and application of the anti-tumor immune drug delivery system, the tumor cell exosome is derived from starved and stimulated tumor cells, the tumor cell exosome is coated with nano drug particles for delivery, and the exosome has the effects of enhancing immune cell infiltration and remodeling an immune microenvironment; due to the homing effect of the exosome, the medicine and the exosome are more easily accumulated in the tumor, and the targeting property is improved; due to coating and camouflage of the biological membrane of the exosome membrane, the exosome membrane is prevented from being recognized, attacked and removed by an organism, and the biological safety is improved, so that the exosome and the nano-drug act on the tumor together to achieve a synergistic effect.

Compared with a porous microsphere in the prior art, the polymer material microsphere with the closed hollow structure is provided with the closed hollow structure or a gas chamber, the surface of the microsphere is smooth on the whole, and acute inflammatory reaction can be avoided. By adopting the microspheres prepared by the invention, the total material implantation amount can be reduced when the same regenerative stimulation point is implanted; and the preparation process is simple, and large-scale production is facilitated.

The invention discloses a grain specific promoter in the early stage of rice filling and application thereof, the promoter is named as proASP1, and the nucleotide sequence of the promoter is shown as SEQ ID NO: 1. The promoter has highly strict spatio-temporal expression specificity, the activation expression of the promoter is only limited in grains in the early stage of rice filling (4-12 days after flowering), especially the expression of genes is efficiently driven in endosperm and aleurone layers, and the expression is not leaked in other tissues such as roots, stems, leaves, leaf sheaths and glumes and grains in the middle and later stages of filling. The invention provides a basic and precious molecular tool for gene function research, quality improvement (such as protein and starch synthesis regulation) and bioreactor development in a key window period of rice grain development, and has important scientific research and application values.

The invention discloses arthrobacter gandavensis N-15 with high selenium resistance and application of the arthrobacter gandavensis N-15, the arthrobacter gandavensis N-15 with high selenium resistance is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242146, and the preservation date is October 9, 2024. The arthrobacter gandavensis N-15 has the advantages that the arthrobacter gandavensis N-15 can be used for preparing the selenium-resistant arthrobacter gandavensis N-15; the selenium resistance of the arthrobacter dabarangensis N-15 reaches 128 mmol / L, and the arthrobacter dabarangensis N-15 has stable adaptability to high selenium concentration and is convenient for biological preparation of nano-selenium. The arthrobacter dawlini N-15 can be used for reducing sodium selenite to generate nano-selenium, and the generated nano-selenium has certain ABTS free radical scavenging capacity and DPPH free radical scavenging capacity, that is, the nano-selenium has relatively good antioxidant capacity. The arthrobacter dawlini N-15 and the nano-selenium produced by the arthrobacter dawlini N-15 can be widely applied to the fields of medicine, food and agriculture.

The invention discloses a mesenchymal stem cell membrane coated kaempferol bionic nano-drug as well as a preparation method and application thereof, and relates to the technical field of targeted drug preparation, the bionic nano-drug is composed of nano-particles containing kaempferol and a mesenchymal stem cell membrane coated outside the nano-particles, the mesenchymal stem cell membrane is derived from umbilical cord mesenchymal stem cells; the particle size of the bionic nano-drug is 167.67 + / -1.84 nm, the PDI is 0.181 + / -0.027, the potential is-5.41 + / -0.74 mV, the encapsulation efficiency is 79.97 + / -0.19%, and the entrapment rate is 7.27 + / -0.02%. According to the invention, the water solubility of the kaempferol is enhanced, the specific enrichment of the kaempferol at the diseased region of the AS can be enhanced, the utilization efficiency of Kae is improved, and the defects of small molecular drugs are overcome; in addition, through nanocrystallization of Kae, direct contact of cells and tissues with Kae can be reduced, and cytotoxicity is reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to peptide slow-release temperature-sensitive hydrogel as well as a composition, a preparation method and application thereof. The composition consists of a hydrogel monomer polymer and biological peptide, wherein the biological peptide is Leucokinin VIII, and the biological peptide is Leucokinin VIII The hydrogel monomer polymer is prepared by mixing and heating polyethylene glycol, lactide and stannous zincate. The preparation method of the peptide slow-release temperature-sensitive hydrogel comprises the following steps: mixing polyethylene glycol, lactide and stannous zincate, and carrying out heating and stirring reaction to prepare a hydrogel monomer polymer; and then mixing the hydrogel monomer polymer with the Leucokinin VIII, so as to obtain the peptide slow-release temperature-sensitive hydrogel. The peptide sustained-release temperature-sensitive hydrogel has the advantages of excellent temperature-sensitive characteristic, injectability, drug sustained-release performance, biocompatibility and biological safety.

The invention belongs to the field of veterinary biological products, and particularly relates to a chicken infectious laryngotracheitis recombinant subunit vaccine as well as a preparation method and application thereof. A core antigen of the vaccine is a recombinant gB trimer glycoprotein (gB-Trimer) modified by genetic engineering, and the protein successfully locks a prefusion conformation with the strongest immunogenicity of the gB protein through strategies of truncation, trimerization motif splicing, dual-stability mutation and the like. The vaccine prepared by the invention can stimulate rapid and lasting neutralizing antibody response, provide 100% clinical protection and effectively block replication and detoxification of the ILTV, has extremely high biological safety compared with a traditional live vaccine, completely eliminates risks of poison dispersion and enhancement, and provides a safe and efficient novel solution for prevention and control of the ILTV.

The invention provides a medical biological rare earth magnesium alloy material. The medical biological rare earth magnesium alloy material comprises magnesium, gadolinium, zinc, zirconium, dysprosium and scandium. According to the rare earth magnesium alloy material provided by the invention, different contents of rare earth gadolinium, dysprosium, scandium, zinc and zirconium are added to improve the casting performance and the processing performance of a melt, and the alloy structure is improved through solid solution strengthening, dispersion strengthening and grain refinement effects, so that the strength and the ductility are improved; meanwhile, the selection design of the alloy elements strictly conforms to the limitation of biotoxicity, and the biological activity of the alloy elements can not be influenced, so that excellent mechanical properties and processability are obtained while good biocompatibility is kept, and the alloy elements play roles in accelerating osteogenesis and promoting bone tissue growth while playing a fixing role; the non-toxicity, the degradability and the high therapeutic property are really realized.

The invention belongs to the technical field of thin film materials, and particularly relates to a transparent ultraviolet-proof composite film as well as a preparation method and application thereof. The transparent ultraviolet-proof composite film provided by the invention is prepared from the following raw materials: acetylated lignin, a halloysite nano material, chitosan and polyvinyl alcohol, the acetylated lignin is loaded on the halloysite nano material. The specific transparent ultraviolet-proof composite film disclosed by the invention has high transparency on the basis of ensuring that the passing rate of ultraviolet light with the wavelength of 200-400nm is less than 5%, the passing rate of visible light with the wavelength of 400-780nm reaches more than 60%, and meanwhile, the transparent ultraviolet-proof composite film also has high mechanical property, is capable of blocking moisture and oxygen, is good in biological safety and is degradable.

The invention discloses a membrane-rupturing antibacterial peptide and application thereof. On the basis of an artificial intelligence screening method, a membrane-rupturing antibacterial peptide KLAKLLKLLLWAQNELDQKK is identified from a proteome of toads, the membrane-rupturing antibacterial peptide has three positive charges, and 50% of amino acid is hydrophobic amino acid. Through experimental tests, the antibacterial peptide can inhibit the growth of 10 different strains including gram negative strains and gram positive strains; meanwhile, the semi-maximum hemolysis concentration and the semi-maximum cytotoxicity concentration of the antibacterial peptide are both greater than 128 mu g / mL, and the antibacterial peptide has good biological safety. The antibacterial peptide plays an antibacterial role by destroying cell membranes, has the characteristic that the drug resistance of bacteria is not easily caused, and is an ideal development object of antibacterial drugs and antibacterial products such as disinfectants, detergents and feed additives.

The application discloses a series of fluorescent phenanthroimidazole-amine copolymers, a preparation method thereof and antibacterial application. The series of compounds are converted into N,N-dimethyl-4-(1H-phenanthro[9,10-d]imidazol-2-yl)aniline with fluorescent properties by taking phenanthrenequinone as a mother body, then the N,N-dimethyl-4-(1H-phenanthro[9,10-d]imidazol-2-yl)aniline is connected with various different aliphatic amines and heterocyclic amines through different length alkyl connectors as lipophilic groups, and a series of fluorescent phenanthroimidazole-amine copolymers are synthesized. The phenanthroimidazole-amine copolymers prepared by the application show strong bacteriostatic effect on staphylococcus aureus and various methicillin-resistant staphylococcus aureus (MRSA), good water solubility and excellent biological safety, and have high yield, simple synthesis method, easy availability and suitability for large-scale production, and are expected to be further developed into semi-synthetic antibacterial drugs in clinical application.

The invention discloses silanized hyaluronic acid as well as a synthesis method and application thereof, and belongs to the technical field of biological material synthesis. The synthesis method of the silanized hyaluronic acid comprises the following steps: dissolving hyaluronic acid in a bio-based solvent to form a hyaluronic acid solution; mixing the hyaluronic acid solution with an amino-containing silane coupling agent, and carrying out a first reaction to form an intermediate reactant; mixing the intermediate reactant with a catalyst, and carrying out a second reaction to form silanized hyaluronic acid; the bio-based solvent is glycerol and / or hexafluorophosphate ionic liquid; the catalyst is an ionic liquid loaded metal oxide. The invention provides the environment-friendly and efficient synthesis method of the silanized hyaluronic acid, and the synthesized silanized hyaluronic acid has higher silicon content, is a water-soluble macromolecule and can meet the application requirements of the silanized hyaluronic acid in cosmetics, health care products and medical auxiliary materials.

The invention discloses a lactobacillus brevis with broad-spectrum bacteriostatic activity and a separation method and application thereof, and belongs to the technical field of microorganisms, in particular to a lactobacillus brevis AW04 with broad-spectrum bacteriostatic activity, which is characterized in that the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, and the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection. The address is Wuhan University Campus, Wuchang District, Wuhan City, Hubei Province; the postcode is 430072; the preservation number of the strain is CCTCC M 20241970; the preservation date is September 13, 2024. The strain provides a novel, safe and efficient strain resource for developing microecological preparations, feed additives or veterinary drugs for preventing and treating bacterial diarrhea of animals such as calves and the like, and has important value for reducing the use of antibiotics in the breeding industry and coping with bacterial drug resistance crisis. The strain can be produced on a large scale by using the existing fermentation technology and is convenient to apply.

The application discloses a novel fullerene hydrogel and a preparation method and application thereof. The fullerene hydrogel is formed by chemical cross-linking of a fullerene derivative shown in formula I and oxidized hyaluronic acid (oxHA). The fullerene-hyaluronic acid hydrogel material provided by the application has a porous framework, a large pore size and a cell loading potential.

The invention relates to a hemostatic adhesive as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The hemostatic adhesive comprises an aldehyde group modified polyethylene glycol high-molecular polymer, an amino high-molecular polymer and a thickening agent, the mass ratio of the aldehyde group modified polyethylene glycol high-molecular polymer to the amino high-molecular polymer to the thickening agent is 1: (0.2-20): (0.1-10). The hemostatic adhesive prepared by the invention is obtained through in-situ polymerization, is easy to inject, and can flow into and fill tissue gaps after injection to complete hemostatic packing; the hemostatic adhesive is high in gel forming speed, can be quickly adhered to the surface of a wound surface when acting on the wound surface, and does not fall off; the hemostatic adhesive physically isolates a wound from the external environment in the wound healing process, prevents wound infection, can be gradually degraded in vivo within 7-10 days, and is non-toxic and harmless.

This invention discloses a highly efficient chiral viral-like nanomedicine carrier for overcoming the gastrointestinal physiological barrier and its construction method, wherein the chiral viral-like nanomedicine carrier is a chiral viral-like mesoporous silica drug-loaded carrier. The construction method of the highly efficient chiral viral-like nanomedicine carrier for overcoming the gastrointestinal physiological barrier includes: using indomethacin as a model drug, and loading indomethacin IMC into a series of mesoporous silica spherical MSNs, viral mesoporous silica VSNs, and chiral viral-like mesoporous silica CVSNs. The chiral viral-like mesoporous silica of this invention has good degradability and biosafety. Its viral topology and chiral modification can enhance the surface and interfacial activity of the carrier and exhibit certain nanoscale chiral recognition capabilities, showing significant advantages in various processes of crossing the intestinal barrier. It can enhance its functionality as an oral drug carrier, has important research value and clinical translation prospects, and provides a reference for the surface and interfacial design of future drug carriers.

This invention discloses a nanoRNA delivery system and its application in the control of Sclerotinia sclerotinia disease, relating to the field of biotechnology. The preparation method of the nanoRNA delivery system includes the following steps: mixing chitosan, sucrose, and aspartic acid uniformly and then subjecting them to a hydrothermal reaction to obtain a quantum dot-chitosan degradation product complex; mixing the quantum dot-chitosan degradation product complex with double-stranded RNA to obtain the nanoRNA delivery system; the double-stranded RNA is used to silence Sclerotinia sclerotinia virulence-related transcription factor genes. The nanoRNA delivery system provided by this invention can effectively protect RNA and exhibits a very high inhibitory effect on Sclerotinia sclerotinia in plants, providing a novel technical solution for the green and precise control of Sclerotinia sclerotinia disease.

The present invention relates to the use of beta-hydroxy-beta-methylbutyric acid (HMB) or a pharmaceutically acceptable salt thereof as an inhibitor of Gasdermin D (GSDMD) in the manufacture of a medicament for the treatment of pyroptosis. HMB modulates pyroptosis in macrophages by blocking palmitoylation of GSDMD and has the potential to be a novel therapeutic agent for the treatment of inflammatory diseases driven by pyroptosis disorders.

This invention belongs to the field of antimicrobial peptide technology, and particularly relates to a broad-spectrum antimicrobial peptide with low hemolytic activity and its applications, as well as a broad-spectrum antibacterial drug. The antimicrobial peptide is named AY6, and its amino acid sequence is shown in SEQ ID NO.1. Antimicrobial peptide AY6 exhibits significant antibacterial effects against both Gram-positive and Gram-negative bacteria, with a bactericidal rate far exceeding that of traditional antibiotics, enabling highly effective responses to bacterial infections. AY6 has low hemolytic activity and good biocompatibility, making it suitable for medical anti-infective applications. The antimicrobial peptide provided in this application has a short sequence, is easy to synthesize chemically, can save on large-scale production costs, and has broad application prospects.

The invention relates to the technical field of microorganisms and the field of food, in particular to lactobacillus plantarum for inhibiting brown stain of soybean paste and application of lactobacillus plantarum. The lactobacillus plantarum is screened from traditional naturally-fermented peasant soybean paste in northeast China, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 30, 2025, and the preservation number is CGMCC No.35048. The lactobacillus plantarum has an obvious browning inhibition effect and can be widely applied to fermented food production.

The application belongs to the field of medical materials, and particularly relates to a metal core enhanced double-barrier piezoelectric nano system and a preparation method and application thereof. The preparation method comprises the following steps: first, a core-shell structure of'metal@mesoporous titanium dioxide@mesoporous barium titanate' is prepared through a step-by-step reaction, and then the target nanoparticles are obtained through lipid wrapping. The system innovatively constructs a double-barrier structure, can self-trigger active oxygen under a physiological pressure of 4 mmHg, realizes endogenous pressure self-driven treatment of malignant ascites, has the advantages of low pressure, high catalytic efficiency, long-term structural stability and good biocompatibility, solves the problems that existing treatment methods are highly invasive and depend on exogenous stimulation, and provides a new safe and efficient technical scheme for the treatment of malignant ascites.

This invention discloses 5α-reductase degrading molecules, their preparation methods, and applications. Four 5α-reductase degrading molecules can induce proteasome-dependent degradation of 5α-reductase, achieving complete clearance of the enzyme protein rather than merely inhibiting its activity, thus obtaining a more durable therapeutic effect. Under equal-dose conditions, the 5α-reductase degrading molecules are more effective than the traditional inhibitor finasteride in treating benign prostatic hyperplasia (BPH). These four 5α-reductase degrading molecules can effectively degrade 5α-reductase protein and significantly inhibit the development of BPH, exhibiting good biocompatibility. This provides a new strategy and candidate drugs for the treatment of BPH, prostate cancer, androgenetic alopecia, acne, and other diseases related to 5α-reductase.

The present invention belongs to the field of genetic engineering technology, and discloses a pSFV-p54 replicon particle and a preparation method and application thereof. The preparation method of the pSFV-p54 replicon particle comprises the following steps: (1) using pSFV-sp6-EGFP plasmid and flag-p54 gene as materials, constructing a pSFV-sp6-p54 plasmid; the flag-p54 gene is as shown in SEQ ID NO: 1; (2) linearizing the SFV-helper auxiliary plasmid and the pSFV-sp6-p54 plasmid respectively and performing in vitro transcription, and co-electrotransferring the obtained mRNA into BHK cells or Vero cells to obtain pSFV-p54 replicon particles. The present invention uses Semliki Forest virus as a vector, inserts the African swine fever p54 gene, and the prepared pSFV-p54 replicon particles can efficiently express the African swine fever virus p54 protein, and have high biosafety, and can be used to develop African swine fever related vaccines.