2692results about How to "High biosecurity" patented technology

The invention discloses a method for preparing a medical porous tantalum material. The method comprises the following steps of: mixing a poly ethanol aqueous solution and tantalum powder to obtain slurry, wherein the mass concentration of the poly ethanol aqueous solution is 2 to 8 percent; injecting the slurry into an organic foam by vibrating and pressurizing, wherein the vibrating frequency is 20 to 80 times/min; drying; degreasing; sintering, namely raising temperature to 1,500 to 1,800 DEG C at the speed of 10 to 20 DEG C/min under the vacuum degree of 10<-4> to 10<-3>Pa, preserving heat for 120 to 240 minutes, cooling to 200 to 300 DEG C along with a furnace, raising temperature to 1,500 to 1,800 DEG C at the speed of 10 to 20 DEG C/min again, preserving heat for 180 to 240 minutes, raising temperature to 2,000 to 2,200 DEG C at the speed of 5 to 10 DEG C/min, and preserving heat for 120 to 360 minutes; cooling; and performing thermal treatment, namely raising temperature to 800 to 900 DEG C at the speed of 10 to 20 DEG C/min under the vacuum degree of 10<-4> to 10<-3> Pa, preserving heat for 240 to 480 minutes, cooling to 400 DGE C at the speed of 2 to 5 DGE C/min, preserving heat for 120 to 300 minutes, and cooling to room temperature along with the furnace. The porous tantalum prepared by the method is very suitable to be used for the medical implant material for replacing bearing bone tissues, and biocompatibility and the mechanical property can be guaranteed simultaneously.

The invention belongs to the technical fields of environmental engineering and bioengineering and particularly relates to a sphingomonas strain and applications thereof in the aspects of shortcut nitrification-denitrification of nitrogen-containing industrial waste water and domestic sewage and treatment of a polluted water source. The sphingomonas strain is separated from a Taihu water in China,is a local strain and has high safety; and the strain can be grown in a basic medium, wherein in the basic medium, CO2 is used as a carbon source and energy or CO2 and an organism are used as a mixedcarbon source and energy, and ammonia nitrogen or nitrate nitrogen is used as a nitrogen source. The bacterium liquid, the dormancy cell and the immobilized strain of the sphingomonas can decompose ammonia nitrogen into nitrite nitrogen and simultaneously can decompose the ammonia nitrogen into nitrogen, can be used as denitrification microorganism for converting the ammonia nitrogen into the nitrite nitrogen and the nitrogen, thereby achieving the shortcut nitrification-denitrification. The strain can rapidly decompose the ammonia nitrogen and is suitable for treating the nitrogen-containingindustrial waste water and domestic sewage as well as polluted water source.

The invention relates to a method for preparing composite biological medical material used to avoid adhering organisms, wherein said film is formed by two layers; the layer used as base material is formed by degradable composite macromolecule polylactic acid, glycolic acid, or their copolymer, treated by static spin technique, to make film fiber into nanometer level; then coats one layer of degradable natural macromolecule chitose or relative derivant on the base film, to be treated to obtain the degradable composite biological medical film; and the invention can add different amounts of medical elasticizer between two layers to adjust the degrade speed. The invention can improve immunity with haemostasis function, etc.

The invention discloses a polysaccharide-dopamine composite biogel and application of the biogel. A preparation method of the biogel comprises the following steps: dissolving 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide sodium in an MES (Methyl Ester Sulfonate) buffer solution with a pH value being 5-6 in an inert gas atmosphere so as to prepare an EDC (ethylcarbodiimide) mixed solution, subsequently adding the EDC mixed solution into a polysaccharide solution for reacting at 20-30 DEG C for 0.5-1 hour, then adding a dopamine solution for further reacting at 20-30 DEG C for 1-4 hours, dialyzing a reactant by taking distilled water as a dialyzate, taking trapped fluid for freezing and drying to obtain the polysaccharide-dopamine composite biogel. The polysaccharide-dopamine composite biogel is simple and effective in modification method and capable of increasing the surface viscidity of the cartilage tissue, reducing loss of therapeutic drugs or implanted cells and facilitating the repairing of the cartilage injury.

An axially substituted silicon-phthalocyanin match and its composition are disclosed, which have high optical spectrum characteristics and can be used as the photosensitizer used for photodynamic therapy to tumor and the diseases other than cancer, photodynamic diagnosis and photodynamic disinfecting.

The invention discloses a bi-component quick haemostatic gel and an application thereof. The gel is composed of natural aldehyde polysaccharide solution in mass concentration of 1-20% and amino modified natural biomacromolecule solution in mass concentration of 1-20% at volume ratio of 1:(0.1-1). According to the invention, the stickiness of the gel can be increased and the gelling time can be reduced in the manner of increasing the aldehyde content in the natural polysaccharide and the concentration of the natural aldehyde polysaccharide solution; the gelling time can be reduced in the manner of increasing the grafting volume of amino group in the amino modified natural biomacromolecule solution and the concentration of the amino modified natural biomacromolecule solution. According to the invention, the modified biomacromolecule and the natural biomacromolecule are adopted, so that the biological safety is high; the use method is simple; the bi-component quick haemostatic gel can be applied to the medical haemostatic field and can form the gel for stopping bleeding within 10-600 seconds.

The invention provides artificial articular cartilage based on autologous cells and a preparation method thereof. The artificial articular cartilage comprises a nano bionic support and hydrosol adhered to the nano bionic support, wherein one or more cytokines are coated into the hydrosol. The invention also provides the method for preparing the artificial articular cartilage, which comprises the following steps: preparing an electrostatic spinning solution, a cytokine-containing hydrosol solution and a crosslinking agent solution; using the crosslinking agent solution to receive electrostatic spinning so as to prepare the nano bionic support; and using an ink jet printer to print the cytokine-containing hydrosol solution on the nano bionic support, and curing the hydrosol to obtain the artificial articular cartilage. The three-dimensional support adopted by the invention has ideal degradation speed and is degraded after the regeneration of an articular cartilage layer, can meet the requirement of actual clinical application, can be completely degraded, and has good abrasion resistance and lubricity so as to be capable of replacing the cartilage before the regeneration of the articular cartilage layer.

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

Belonging to the technical fields of biology and food, the invention relates to a preparation method of an active lactobacillus pickled vegetable. The method provided in the invention includes the technological steps of: A. loading a pickled vegetable liquor into a jar; B. filling the jar with vegetables; C. conducting fermentation at a constant temperature of 12-18DEG C; D. taking the fermented vegetables out of the jar, and carrying out blending and packaging. After a finished product is obtained by subjecting the pickled vegetable liquor to first round pickling, a direct vat set lactobacillus starter activating solution prepared from a direct vat set lactobacillus starter and glucose is added into the pickled vegetable liquor in the jar, and they are mixed uniformly for a second round of circulating pickled vegetable preparation. By adopting a reasonable vegetable proportion and a good flavor chilli vegetable fermented pickling liquor, the method provided in the invention well keeps the inherent flavor of the vegetable and the fermented flavor, and realizes standardized preparation of the active lactobacillus pickled vegetable, enhances the quality and homogeneity of the product, substantially improves the biosecurity of the product, and achieves a best flavor and a most suitable taste, thus further realizing upgrading and industrialized, large scale and standardized production of traditional active lactobacillus pickled vegetables.

The invention provides a cellulose/two-dimensional layered material composite hydrogel. The composite hydrogel comprises a cellulose three-dimensional network structure and a two-dimensional materialsupported in the cellulose three-dimensional network structure, wherein the two-dimensional layered material is a two-dimensional transition metal carbide, nitride, or carbonitride. According to the composite hydrogel provided by the invention, the two-dimensional layered material can be stably supported in the composite hydrogel system and is not easy to agglomerate; and the composite hydrogel has good compatibility with a biological fluid, and the advantages of full biodegradability and high biological safety, and can be used in the field of biomedicines. The invention also provides a preparation method of the composite hydrogel.

The present invention discloses a chitosan nanoparticle suspension preparation method and application thereof. The chitosan nanoparticle suspension preparation method comprises the following steps of: (1) dissolving a chitosan into a glacial acetic acid aqueous solution to prepare a chitosan aqueous acetic acid; (2) under stirring, adding a tripolyphosphate sodium aqueous solution dropwise to obtain a coarse chitosan nano suspension; and (3) after filtering the coarse chitosan nano suspension, processing homogeneously the filtered suspension with high pressure to obtain a nano chitosan suspension with a uniform particle size distribution. The invention has the advantages of simple operation, good scale-up preparation reproducibility, and low production cost, and is applicable to industrial production. The prepared nanoparticles are of a uniform morphology, small in particle diameter and applicable to preparing products with functions of antibiosis, hemostasis and promoting tissue repair.

The invention relates to a medical nano-fiber sponge material, of which the porosity is 90-98 percent and the diameter of a nano-fiber is 50-1,000 nanometers. The medical nano-fiber sponge material consists of the following components in percentage by mass: 0.5-8.5 percent of bioactive glass fine particles, 65-88 percent of gelatin, 0.2-5.0 percent of hyaluronic acid, 0.2-5.0 percent of chitosan and the balance of water. A preparation method of the medical nano-fiber sponge material comprises the following steps of: adding bioactive glass fine particles and ethanol into a gelatin aqueous solution, and stirring uniformly; performing low-temperature phase separation and freeze drying to obtain nano-fiber sponge obtained by compounding gelatin and bioactive glass fine particles; and performing soaking, refrigerating and freeze drying with a hyaluronic acid solution and a chitosan solution in sequence under a negative pressure condition to obtain a porous composite sponge material in which nano-fibers are crosslinked and coated statically with hyaluronic acid and chitosan. The nano-fiber sponge has excellent bioactivity, bacteriostasis and mechanical property, and can be widely applied in the fields of regenerative repair of various skin wounds and skin tissue engineering.

The invention relates to a heptamethine indocyanine dye containing N-fatty esters or N-fatty amide side chains, a synthetic method thereof and applications thereof in tumor targeting imaging and treatment. The heptamethine indocyanine dye which possesses or individually possesses near infrared absorption, fluorography and antineoplastic activity allows present research and development ideas and treatment levels of tumor targeting treatment medicaments to be greatly improved, and has great significances to aspects of early discovery, control and the like of various tumors.

The invention discloses a method for synthesizing gemini long chain alkyl quaternary ammonium salts by enabling long chain alkyl tertiary amine mixture to be reacted with dibromo hydrocarbon or epoxy chloropropane and an application as a drag reduction and energy conservation agent of a circulating water system. The method comprises the following steps: stirring and dissolving the long chain alkyl tertiary amine mixture in an organic solvent, and heating to reflux temperature; slowly adding dibromoethane or epoxy chloropropane for generating mixture of asymmetric and symmetric gemini long chain alkyl quaternary ammonium salts; stopping the reaction when the tertiary amine conversion rate is above 75%, and performing vacuum evaporation for recovering the solvent; and using deionized water to dissolve distillation products, and oxidizing the non-converted raw material to tertiary amine oxide with hydrogen peroxide at the temperature of 60-80 DEG C. A gemini quaternary ammonium salt surfactant is used as main molecules of the drag reduction agent, an organic acid corrosion inhibitor is used as auxiliary ions, the molar ratio of the main molecules to the auxiliary ions is 1: (2.4-6.0), then the drag reduction agent which is applicable to the range of 5-95 DEG C is prepared, and the drag reduction efficiency of the drag reduction agent to the circulating water system achieves 40%-70%, and the corrosion inhibition efficiency achieves 60%-95%. By adopting the gemini long chain alkyl quaternary ammonium salt surfactant, the problems of strong corrosion, great irritation and a narrow range of applicable temperature of the traditional long chain alkyl quaternary ammonium salt surfactant can be overcome.

The invention provides a collagen matrix freezing gel used for biomedical materials and a preparation method thereof. The collagen matrix freezing gel is the collagen extracted from skins or tendons of healthy domestic animals using the enzyme method with the molecular weight of 280 to 320kDa and a well maintained triple helical structure; the collagen reacts for 1 to 7 days in the condition of low temperature of 0 to minus 50 DEG C in a die after through cross linked and modified reaction with hydroformylation polysaccharide, then extrudes the die and is defrosted, thus forming the collagen matrix freezing gel. The preparation of the collagen matrix freezing gel of the invention in particular relates to the extraction of the collagen, the preparation of the hydroformylation polysaccharide and the synthesis of hydroformylation polysaccharide-collagen matrix freezing gel. The hydroformylation polysaccharide-collagen matrix freezing gel prepared by the invention improves the mechanical property, thermal stability and anti-enzyme degradation, etc., of pure collagen gels, and the freezing gel has the advantages of porosity, plasticity, hydrophilic property and non-toxicity, and can be used as biomedical materials such as bio-scaffold, cell cultivation, drug controlled release and biological dressings.

The biological material film with porous structure is medical film of polyamide or composite polyamide/nanometer osteolith material with through pores in gradually changed sizes, and has one compact side with fine pores in diameters of 0.01-30 microns and the opposite loose side with large pores in diameters of 30-300 microns. During its preparation, solvent with high volatility is used as the dispersant, medical polyamide or composite polyamide/nanometer osteolith material is heated and stirred to form the filming liquid, and film is formed and washed with deionized water to obtain the biological film. The porous biological film has controllable porous structure, and excellent mechanical performance and biological performance, and may be used widely as medial separating film, medicine slow releasing film, tissue regeneration inducing film, etc.

The invention provides an antineoplastic polypeptide nanometer drug, and a preparation method and application thereof. The antineoplastic polypeptide nanometer drug comprises amphipathic antineoplastic polypeptides and acid responsiveness functional molecules link-coupled with the amphipathic antineoplastic polypeptides. Amino acid is taken as the raw material to synthesize amphipathic polypeptide molecules, and then acid responsiveness functional molecules are introduced into the amphipathic antineoplastic polypeptide molecules to be subjected to self-assembling to form the antineoplastic polypeptide nanometer drug. The antineoplastic polypeptide nanometer drug provided by the invention has good biocompatibility, smaller toxic and side effects, acid responsiveness, high bioavailability and high biosecurity. The preparation method provided by the invention is simple, covalent bonds are not produced in the self-assembling process, reverse reaction is avoided, and the prepared antineoplastic polypeptide nanometer drug has a broad application prospect.

The invention discloses a graphene oxide/polymer composite antibacterial material and a preparation method thereof. The composite antibacterial material is characterized in that a polymer is subjected to surface modification and then is combined with graphene oxide dispersed in water, thus obtaining a graphene oxide antibacterial coating bonded on a polymer matrix, wherein the thickness of the antibacterial coating is 1 to 50nm. The preparation method of the graphene oxide/polymer composite antibacterial material comprises the following steps: firstly, performing ultrasonic cleaning on the polymer; secondly, performing surface modification on the polymer subjected to ultrasonic cleaning; thirdly, immersing an activated polymer matrix into a coupling agent solution and soaking; fourthly, taking out the soaked polymer, leaching with deionized water, then putting into a graphene oxide suspension liquid for 0.2 to 24 hours, taking out, leaching and naturally drying in air to obtain the graphene oxide/polymer composite antibacterial material. The invention designs and prepares the composite antibacterial material by taking graphene oxide as an antibacterial ingredient; the composite antibacterial material has a transparent antibacterial layer, is excellent in antibacterial property and is firmly bonded with the matrix.

The invention relates to chemical crosslinking type glucan hydrogel and a preparation method thereof, and belongs to the technical field of biomedical functional polymer materials. The preparation method comprises the steps that glucan is dissolved in water at room temperature and then placed in an ice-water bath, and sodium periodate is added to obtain a mixed solution; the mixed solution is stirred for reaction for 2-6 h on the room temperature and dark conditions; the stirred solution is dialyzed with a regenerated cellulose dialysis bag in deionized water, and after dialysis is completed, freeze drying is performed to obtain partial hydroformylation glucan solids with the hydroformylation degree of 40-60 percent; the obtained partial hydroformylation glucan solids are dissolved in the deionized water to obtain an aqueous hydroformylation glucan solution, a multi-amino crosslinking agent is added in the aqueous hydroformylation glucan solution to be mixed evenly at the room temperature, and then the glucan hydrogel is prepared. The chemical crosslinking type glucan hydrogel is prepared by taking natural glucan macromolecules with the good biosafety as a material basis through chemical crosslinking of the partial hydroformylation glucan and the multi-amino crosslinking agent.

Belonging to the technical fields of biology and food, the invention relates to a preparation method of an active lactobacillus pickle pickling liquor. The method includes: taking a fermentation pickling solution of pickled pepper, a fermentation pickling solution of pickled capsicum frutescens and cold boiled water as raw material water, subjecting the pickling solutions to rough filtration, and mixing them with the cold boiled water uniformly, then carrying out cooling and high speed centrifugation degerming, and adding a direct vat set lactobacillus starter, thus obtaining the pickling liquor. The method provided in the invention realizes standardized preparation of pickling liquor, ensures the viable count of lactobacilli in the pickling liquor, endows the newly prepared pickling liquor with the flavor of aged pickling liquor and better flavor. At the same time, the use problem of the pickling solutions of pepper and capsicum frutescens is solved, the environmental pollution caused by emission and the potential safety hazard caused by repeated use in the next year can be avoided, so that the biological safety, quality and homogeneity of the product are significantly improved. Further, upgrading and industrial, large-scale and standardized production of traditional active lactobacillus pickles can be realized.

The invention belongs to the new technical field of a drug preparation and in particular relates to a precursor liposome containing a glycyrrhetinic acid and a method for preparing the same. The precursor liposome containing the glycyrrhetinic acid consists of glycyrrhetinic acid, phospholipids, cholesterol, a surfactant and a water-soluble material; a suspension solution of the glycyrrhetinic acid liposome is prepared by an ethanol injection method or a thin-film dispersion method; and solid liposome powder is prepared by a freeze drying method or a spray drying method. The precursor liposome has simple process and good repeatability, is suitable for industrialized production; through drug administration by intravenous injection, the precursor liposome has long-circulating function, can remarkably reduce the toxicity of drug and achieve the function of prolonging the drug effect; and through oral administration, a solid preparation of the liposome can increase absorption and improve bioavailability by three to five times.

The invention discloses an oral composition containing Ectoine and hyaluronic acid and application of the oral composition. The oral composition is prepared from, by mass, 0.1-5% of high molecular weight hyaluronic acid or salt thereof, 0.1-5% of low molecular weight hyaluronic acid or salt thereof, 0.01-1% of tea polyphenol, 1-3% of xylitol, 0.1-1% of Ectoine and the balance water, and the totalpercentage is 100%. Accordingly, the Ectoine, different molecular weights of hyaluronic acid or salt thereof, tea polyphenol and xylitol are compounded, the bio-safety is high, the synergetic effect is achieved, the effects of nursing wound surfaces, diminishing inflammation, promoting healing and the like are achieved, and the oral composition is applicable to oral care application such as oral care after cancer chemotherapy and oral ulcer care and can also be applied to the field of medical products, medical equipment and disinfection products.

The invention provides a targeted photothermal black phosphorus nano-preparation, which is prepared from a black phosphorus nano-slice, polyethylene glycol absorbed on the surface of the black phosphorus nano-slice by means of electrostatic attraction, and folic acid connected to the polyethylene glycol by means of an amide bond, wherein one end of the polyethylene glycol is amino, and the other end of the polyethylene glycol is imino; nitrogen atom of the imino and carbonyl carbon atom of the folic acid are connected with each other so as to form the amide bond; the folic acid is exposed at the outermost layer of the nano-preparation. The targeted photothermal black phosphorus nano-preparation is high in stability, good in target recognition ability and excellent in light-heat property, is capable of effectively killing cancer cells, and can be used for performing targeted photothermal treatment on cancers at the cellular level. The invention also provides a preparation method and application of the targeted photothermal black phosphorus nano-preparation.