40 results about "Paromomycin" patented technology

The invention discloses a tetrahymena transgenic carrier containing HSP70 promoter and GFP and preparation method and use thereof. Plasmid T-GFP is constructed by using plasmid rDNA-GFP in the methods such as enzyme cutting and PCR enzyme cutting site mutation; thermophile tetrahymena genome DNA is used for constructing plasmid T-HSP702 in the methods such as PCR augmentation and clone; Plasmids T-GFP, T-HSP702 and pD5H8 are used for construction and finally obtaining plasmid HSP702-GFP-pD5H8 by enzyme cutting and connection. The carrier contains HSP702 promoter, thereby being capable of realizing high-efficient expression of exogenous gene; the carrier contains replaceable exogenous gene gfp so that an exogenous gene expression system is realized for tetrahymena by replacing different exogenous genes; with the sieving effect of paromomycin medicine, the carrier replaces most rDNAs originally in the tetrahymena so that the exogenous genes are largely increased in the tetrahymena so as to realize genetic transformation of target genes. The method in the invention is easy and has convenient operation, and the carrier can be used for detection of environmental tributyl tin in transfection in tetrahymena.

A new class of paromomycin-derived aminoglycosides, which exhibit efficient stop-codon mutation suppression activity, low toxicity and high selectivity towards eukaryotic cells are provided. Also provided are chemical and chemo-enzymatic processes of preparing these paromomycin-derived aminoglycosides and intermediates thereof, as well as pharmaceutical compositions containing the same, and uses thereof in the treatment of genetic disorders.

The invention discloses a specific monoclonal antibody capable of identifying neomycin, amikacin and paromomycin and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting neomycin, amikacin and paromomycin. The monoclonal antibody is secreted by a hybridoma cell EDC/5G04 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201144. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples and the like. Compared with the prior art, the monoclonal antibody, the ELISA method and the kit have the following main advantages that the prepared monoclonal antibody can simultaneously identify neomycin, amikacin and paromomycin, thus improving the detection efficiency of the prior art; the animal tissue sample treatment method is simple and short in time; and the ELISA method and the kit also have the characteristics of high detection sensitivity, good precision and good accuracy.

The invention discloses a compound microbial fertilizer for preventing and treating take-all disease of wheat and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer consists of the following raw materials: streptomyces catenulae, streptomyces albolongus, bacillus flexus, bacillus aryabhattai and trichoderma brevicompactum. The strains of the invention, on the basis of the comprehensive utilization of microbial inter-species or intra-species antibiosis, competition, hyperparasitism and bacteriolysis effects or by inducing the wheat to generate disease resistance by virtue of secondary metabolites, can generate various antibiotics, such as proceomycin, neomycin E, catenulin, diparomomycin and the like, so as to inhibit pathogenic bacteria of the take-all disease of wheat; the five strains adopted by the invention are directly separated from soil and have a rhizosphere growth promoting effect; the strains can promote reproduction and growth on crop surfaces or in plants or soil, and meanwhile, the strains can take an effect on nitrogen fixation and can promote the directional secretion of some secondary metabolites from plant rhizosphere; and the strains can enhance nutrition absorption of plants and stimulate plant growth, so that the effects of the fertilizer are achieved.

This invention discloses a method of inducing expression of full-length collagen 7 in cells that contain nonsense mutations in the COL7A1 gene by treating the cell with an aminoglycoside such as G418, gentamicin, and paromomycin. Also provided is a method of treating DEB due to nonsense mutation in the COL7A1 gene by administering a composition containing an effective amount of an aminoglycoside. Also provided is a novel composition useful to treating conditions due to nonsense mutation in the COL7A1 gene, containing an aminoglycoside, a C7, a min-C7 or both; and a pharmaceutically acceptable carrier.

The invention discloses a method for improving output of tetraene macrolide antibiotics and belongs to the technical field of microorganisms. According to the method, 100mu g/mL paromomycin is added in a solid medium which is used for culturing Streptomyces diastatochromogenes D to obtain a Streptomyces diastatochromogenes mutant strain which has relatively good resistance to the paromomycin; the capacity of synthesizing the tetraene macrolide antibiotics of the mutant strain is stronger than that of the Streptomyces diastatochromogenes D.

The invention discloses a colloidal gold test strip, which is formed by laminating and sticking an adsorption pad, a cellulose nitrate film, a gold label pad and a sample pad on a plastic lining plate in sequence, wherein the cellulose nitrate film is provided with a detection line and a quality control line; the gold label pad is coated with a monoclonal antibody which can be used for identifying neomycin, amikacin and paromomycin of a colloidal gold label; the detection line is coated with a neomycin coating antigen; and the quality control line is coated with a goat or rabbit anti-mouse IgG (Immunoglobulin). The invention further discloses a use method of the colloidal gold test strip and an application of the colloidal gold test strip to the detection of neomycin residual. The colloidal gold test strip can be applied to rapid detection of neomycin residual in tissue samples of milk, chicken, pork, shrimp, beef, mutton, pork liver and the like, and has the minimum detection limit of 500mu.g/L, high specificity and high accuracy.

The invention provides a hybridoma cell line C1 capable of secreting an anti-paromomycin monoclonal antibody and an application of the hybridoma cell line C1 and belongs to the technical field of immunochemistry. A mouse monoclonal cell line C1 is prepared with a conventional cell fusion technology, and is preserved in the CGMCC (China General Microbiological Culture Collection Center) with the preservation number being CGMCC No. 12025. The monoclonal antibody secreted by the cell line is determined by indirect competitive enzyme-linked immunosorbent assay, and the IC50 (half maximal inhibitory concentration) for paromomycin and the IC50 for neomycin are 0.61 mu g / L and 2.43 mu g / L respectively. The recovery rate range for paromomycin and neomycin in animal food is 64.56%, 105.85% and 54.08%-100.55%. Raw materials are provided for immunoassay of paromomycin residues in animal food, and the monoclonal antibody has practical application value.

The invention provides novel application of paromomycin to preparation of medicine for treating trichomonas vaginitis.The application of paromomycin to preparation of medicine for treating trichomonas vaginitis is implemented by inhibiting trichomonas vaginalis.Compared with the prior art, the application of paromomycin to preparation of medicine for treating trichomonas vaginitis has the advantages that paromomycin of low concentration has a good killing action on trichomonas vaginitis, has an obvious effect on inhibition of trichomonas vaginitis and can be used for preparing medicine for treating trichomonas vaginitis so as to solve the problems of drug resistance and high side effect existing in an existing drug.

The invention discloses a Tetrahymena expression vector of chitinases and its application in expressing chitinases. The invention provides an expression cassette, which includes an HSP70-2gene promoter as shown in the 9th-1126th nucleotides from the 5' terminal of sequence 1, an encoding gene of chitinases in sequence 2 and an HSP70-1 gene termination signal as shown in the 3835th-4312th nucleotides from the 5' terminal of sequence 1. The invention also provides a recombinant plasmid containing the expression cassette. When transferred to a Tetrahymena through electroporation, the recombinantplasmid can, under the drug screening effect of paromomycin, replace most of the original rDNA in the Tetrahymena, and multiply an exogenous gene in the Tetrahymena massively, thus realizing genetic transformation of a target gene. Under heat shock conditions, the HSP70-2 strong promoter can start the high level expression of CBD3 gene, and the expressed chitinases have bioactivity (able to hydrolyze chitin). In conclusion, the expression vector of the invention has wide application prospects.

The invention discloses application of a chlamydomonas reinhardtii RFC1 gene in the regulation of cadmium tolerance of chlamydomonas reinhardtii and belongs to the fields of biotechnology and environment pollution control. A chlamydomonas reinhardtii mutant strain capable of resisting cadmium chloride obviously is acquired by randomly inserting an aphVIII segment (SEQ ID NO: 3) containing a paromomycin resistance gene into chlamydomonas, and further identification discovers that the cadmium resistance of the mutant strain is caused by inserting the aphVIII segment into the RFC1 gene (the CDS sequence is as shown in SEQ ID NO: 1). By reducing the expression of the chlamydomonas reinhardtii RFC1 gene, the RFC1 gene is deactivated or deleted so as to improve the cadmium tolerance of the chlamydomonas reinhardtii; the chlamydomonas reinhardtii strain with enhanced cadmium tolerance and acquired through a gene engineering method can be applied to the monitoring and treatment of a cadmium-polluted water area and provides a new direction for sewage treatment.

The invention discloses a method for increasing yield of toyocamycin through screening of united drug-resistant mutant strains and belongs to the field of microbial technologies. According to the method, Streptomyces diastatochromogenes D mutant strains generating drug resistance to low-concentration streptomycin, high-concentration streptomycin and paromomycin are obtained through screening of the united drug-resistant mutant strains, and the toyocamycin productivity of the obtained mutant strains is remarkably improved when compared with that of Streptomyces diastatochromogenes D.

The invention discloses application of Chlamydomonas reinhardtii g3280.t2 gene for regulating and controlling cadmium tolerance of Chlamydomonas reinhardtii, and belongs to the fields of biotechnologyand environmental pollution control. A Chlamydomonas reinhardtii mutation strain obviously sensitive to cadmium is obtained by inserting the aphVIII fragment (SEQ ID NO:3) containing a paromomycin resistance gene into chlamydomonas at random, and further identification discovers that the cadmium sensitivity of the mutation strain is caused by the fact that the aphVIII fragment is inserted into the g3280.t2 gene (CDS sequence is shown as SEQ ID NO: 1). By reducing the expression of the Chlamydomonas reinhardtii g3280.t2 gene, the g3280.t2 gene is inactivated or the cadmium tolerance of Chlamydomonas reinhardtii can be reduced by losing the gene; and the cadmium sensitive Chlamydomonas reinhardtiis train obtained through gene engineering means can be applied into water environments for monitoring and detection of cadmium pollution through biological means, and then a novel biological material is provided for sewage detection.

The invention discloses application of chlamydomonas reinhardtii VMPL1 gene to the regulation of the cadmium tolerance of chlamydomonas reinhardtii, and belongs to the fields of biotechnology and environmental pollution control. According to the invention, an aph VIII segment (SEQ ID NO:3) containing paromomycin resistance gene is randomly inserted into chlamydomonas, chlamydomonas reinhardtii mutant strain obviously sensitive to cadmium is obtained, and through further identification, the fact that the cadmium resistance of the mutant strain is caused because the aph VIII segment is insertedinto the VMPL1 gene (CDS sequence is as shown in SEQ ID NO:1) is discovered. By reducing the expression of the chlamydomonas reinhardtii VMPL1 gene to enable the VMPL1 gene to be inactivated or deleted, the VMPL1 gene can improve the cadmium tolerance of chlamydomonas reinhardtii; the chlamydomonas reinhardtii strain with enhanced cadmium tolerance obtained through a gene engineering method can beapplied to the mentoring and control of cadmium polluted waters. According to the invention, a novel thought and a biological material are provided for sewage treatment.

The invention discloses an application of Chlamydomonas reinhardtii Cre01.g046237.t1.1 gene in regulating cadmium tolerance of Chlamydomonas reinhardtii. Through inserting aph VIII segment containinga paromomycin resistance gene in Chlamydomonas at random, the Chlamydomonas reinhardtii mutated strain capable of resisting cadmium chloride obviously is acquired, the further identification finds that the cadmium resistance of the strain is caused by inserting the aph VIII segment (SEQ ID NO: 3) in Cre01.g046237.t1.1 gene. Through reducing the expression of the Chlamydomonas reinhardtii Cre01.g046237.t1.1 gene (the CDS sequence is shown as SEQ ID NO: 1), the deactivated or deficient gene can improve the cadmium tolerance of Chlamydomonas reinhardtii; the Chlamydomonas reinhardtii strain withenhanced cadmium tolerance acquired through gene engineering method can be applied to the monitoring and governing of a cadmium polluted water area, and provides a new direction for sewage governing.

Disclosed is the continuous supplementation of poultry feeding stuffs with paromomycin and the resulting effects of prophylaxis against histomoniasis, ensuing decrease of mortality and the reduction of horizontal spreading of the disease. Also observed are improved zoo-technical performances, increase in weight gain and feed efficiency, both in healthy as well as diseased birds.

The invention discloses application of a chlamydomonas reinhardtii VPS9 gene in regulating and controlling cadmium resistance of chlamydomonas reinhardtii, and belongs to the technical field of biologics and the field of sewage improvement. According to the application, an aphVIII fragment (SEQ ID NO:3) with a paromomycin resistance gene is randomly inserted into chlamydomonas, then a chlamydomonas reinhardtii mutant alga strain which is remarkably sensitive to cadmium is cultured, and further identification shows that the cadmium sensitivity of the mutant alga strain is caused since the aphVIII fragment is inserted into a VPS9 gene (a CDS (Cadmium Sulfide) sequence is shown in SEQ ID NO:1 in the specification). By reducing expression of the chlamydomonas reinhardtii VPS9 gene, deactivating the VPS9 gene or by deleting the VPS9 gene, the cadmium resistance of the chlamydomonas reinhardtii can be degraded; a cadmium-sensitive chlamydomonas reinhardtii strain obtained by means of geneticengineering can be applied to monitoring and detection on cadmium polluted water areas, and novel ideas and biological materials can be provided for sewage treatment by using a biological method.