3 results about "Paromomycin" patented technology

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

This invention discloses a method for constructing an engineered algal strain of high-intensity sweet protein variants derived from *Chlamydomonas reinhardtii* and its application. First, the sweeteners Thaumatin and Brazzein are genetically modified. Then, through codon optimization based on *Chlamydomonas reinhardtii* preference, the encoding genes for CrThaumatin and CrBrazzein are synthesized and cloned into the expression vector pGM6. The recombinant expression vector is introduced into wild-type *Chlamydomonas reinhardtii* sp. algal strains. Using the paromomycin resistance marker carried by the expression vector, the engineered algal strains are successfully obtained. The engineered algal strains are fermented to produce dried, stable *Chlamydomonas reinhardtii* powder rich in the target sweet protein. Finally, oral administration tests after protein purification show that its sweetness is 3-5 times that of natural Thaumatin and Brazzein proteins.

This invention discloses a method for constructing an engineered algal strain of high-concentration sweetness-inducing protein variants from *Chlamydomonas reinhardtii* and its applications. First, the protein coding sequences of the sweetness-inducing proteins Miraculin and Neoculin are modified and optimized to obtain optimized CrMiraculin and CrNeoculin gene sequences. These sequences are then cloned into the expression vector pGM6 to construct a recombinant expression vector. This recombinant expression vector is introduced into wild-type *Chlamydomonas reinhardtii* strains, and the transformed algal strains are selected using a paromomycin resistance marker carried by the vector. The obtained engineered algal strains can stably inherit and efficiently express the target sweetness-inducing proteins. The engineered algal strains are then fermented to obtain *Chlamydomonas reinhardtii* algal powder rich in CrMiraculin and CrNeoculin sweetness-inducing proteins. Finally, after protein purification, mixing with acidic substances can induce a sweet taste, thus demonstrating significant application potential in the food, condiment, or health product fields.