11 results about "Neomycin" patented technology

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

Herein, an exploration was made of the potential therapeutic effect of injecting neomycin into the lung immediately after traumatic brain injury (TBI). It was hypothesized that modulation of the pulmonary microbiome may help alleviate neuroinflammation and cognitive impairment following TBI. Traumatic brain injury induced neutrophil infiltration into the alveoli along with interstitial pulmonary edema, and reduced levels of caspase-1, IL-1β, and RAGE. TBI rats treated with neomycin exhibited a significant decrease in Acinetobacter levels in BAL and a decrease in α-diversity Shannon index compared to rats without neomycin. The neomycin-administered TBI rats exhibited a higher discrimination index in a novel object recognition test, indicating that cognitive impairment was alleviated. While serum inflammatory cytokines in injured mice tended to increase for up to 7 days, such increases were not observed from day 3 in neomycin-administered TBI rats. Injection of neomycin into the lung immediately after TBI effectively alleviated neuroinflammation and cognitive impairment through modulation of the pulmonary microbiome.

The invention discloses a multi-immunochromatography test strip with unified detection limit for detecting four aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a colloidal gold pad, a sample pad and a PVC (polyvinyl chloride) bottom plate, wherein a gold-labeled antibody is fixed on the surface of the colloidal gold pad, and when a sample flows through the colloidal gold pad, the gold-labeled antibody is dissolved and serves as a tracer molecule to migrate along with a liquid phase; four detection areas T1, T2, T3 and T4 and a quality control line area C are arranged on the detection pad, a kanamycin antigen, a gentamicin antigen, a neomycin antigen and a spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse antibody is sprayed on the quality control line area C. According to the present invention, the expensive reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting kanamycin, gentamicin, neomycin and spectinomycin, and the detection limits can achieve 5 ng / mL.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.

The invention relates to an aptamer for specifically detecting neomycin and application of the aptamer, and belongs to the technical field of detection. Firstly, a novel aptamer (the affinity reaches 1.17 + / -0.32 mu M) with good affinity to neomycin is designed, and the aptamer has no reaction to other interfering antibiotics such as chloramphenicol (CAP), oxytetracycline (OTC), enrofloxacin (ENR), azithromycin (AZT), roxithromycin (RXM) and ciprofloxacin (CIP) and has good specificity. Secondly, two fluorescence signal probes of MOF coated FITC and AgNCs are also synthesized, a ratio type fluorescence sensing system is constructed, high-precision and high-sensitivity detection of the target neomycin is realized through a double-signal ratio, and background signal influence caused by environmental interference and fluorescent dye leakage is effectively eliminated.

The present application discloses a genetically engineered strain for producing neomycin B0, which is obtained from a mutant strain of the starting strain G. lozoyensis ATCC 74030 by modifying specific genes, and has an improved yield of neomycin B0 compared with the starting strain, wherein the genetic modification comprises overexpression of GLcs gene and overexpression of GLhyd gene, and preferably, the genetic modification further comprises one or more genetic modifications selected from the group consisting of knockout of GL6ma gene, knockout of GLpyrE gene, overexpression of GLp450-1 gene, overexpression of GLp450-2 gene, and overexpression of GLhyp gene. The present application also discloses a method for preparing the genetically engineered strain, and a method for preparing neomycin B0.

The invention provides a related medicine for treating senile diseases and application, the related medicine is used for inhibiting or removing methylmalonic acid in a body, and the related medicine is selected from at least one of coenzyme A, hydroxycobalamin, levocarnitine, betaine, carglutamic acid, metronidazole, neomycin, ACSF3 protein or an activator. According to the invention, the level of methylmalonic acid in vivo can be significantly reduced by using a removal technology (hemodialysis or application of a methylmalonic acid scavenger); after the methylmalonic acid is removed, the condition of senescence diseases can be obviously improved; mSC rejuvenation can be significantly increased by inhibiting methylmalonic acid.

The method comprises the following steps: adsorbing, washing and desorbing neomycin fermentation liquor through an adsorption system, and then concentrating to obtain desorbed concentrated liquor; the preparation method comprises the following steps: washing with low-concentration ammonia water, then dynamically adsorbing to cationic resin after dilution, washing with low-concentration ammonia water, then connecting weak positive resin and anion resin in series, washing with medium-concentration ammonia water, then desorbing with high-concentration ammonia water, and finally, concentrating, carrying out salt conversion and decoloration, and carrying out spray drying to obtain the Feramectin sulfate. According to the method, the conventional thought of primary adsorption and gradient washing of resin is broken through, weak positive and low-particle-size resin is selected for secondary dynamic adsorption and desorption, the better separation degree is achieved, the production cost is reduced, and the high-purity fralamectin sulfate is prepared.

This invention relates to the field of microbial detection technology and discloses a partitioned antimicrobial susceptibility testing plate and its application. The plate comprises a plate body and a plurality of microwell reaction units disposed on the plate body. The plate body is spatially divided into at least two independent detection zones, including a first detection zone and a second detection zone. A first selective culture system is pre-installed within the microwell reaction units of the first detection zone. The first selective culture system includes neomycin and at least one antimicrobial drug concentration gradient targeting Gram-negative bacilli. The partitioned antimicrobial susceptibility testing plate provided by this invention enables direct and simultaneous antimicrobial susceptibility testing of mixed samples of Gram-negative bacilli and Gram-positive cocci.