20 results about "Neomycin" patented technology

The application relates to the technical field of image processing, in particular to a new neomycin spectrum image data processing method based on a deep learning model, which comprises the following steps: obtaining initial new neomycin spectrum data, inputting each new neomycin spectrum into a target deep learning model to obtain corresponding image feature vectors; and obtaining new neomycin classification cluster data according to the image feature vectors; it is known that, by using the time sequence relationship degree between the image features of any two initial new neomycin images, the initial new neomycin images are classified based on the time sequence relationship degree, the time relationship between the new neomycin images can be analyzed, the data of the new neomycin changing with time is established, and the data quantity of the new neomycin images is improved.

The invention belongs to the technical field of medicines, and discloses a near-infrared fluorescent probe Cy-NEO-Leu for leucine aminopeptidase responsiveness as well as a preparation method and application of the near-infrared fluorescent probe Cy-NEO-Leu. The parent nucleus of the probe is of a heptamethyl cyanine structure, and the probe has high molar absorption coefficient, high fluorescence quantum yield and excellent phototherapy characteristics. The introduction of a neomycin group can increase the water solubility of the probe, and a neomycin multi-ammoniation structure exists in a polycation form under physiological conditions and can target negatively charged cell walls of gram-negative bacteria, so that the probe Cy-NEO-Leu is enriched around the bacteria, can be specifically recognized by leucine aminopeptidase and can be hydrolyzed and activated; and photo-thermal and photodynamic sterilization effects are achieved under laser irradiation. Due to the good biocompatibility, the remarkable active oxygen generation capacity, the excellent photo-thermal conversion efficiency and the unique property of accurately targeting bacteria, the Cy-NEO-Leu is expected to play an important role in a photo-thermal / photodynamic combined treatment scheme, and is used for resisting diseases caused by bacteria and biological membranes and accelerating the healing process of wounds.

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

Based on the surface plasmon resonance enhancement of gold triangle, the specific detection of neomycin is involved in the field of analysis and detection. The characteristics of the tip of the gold triangle are easy to gather charges, which can regulate the surface plasmon of silver sol and induce the adsorption orientation of neomycin on the surface of silver sol, causing the displacement of its Raman characteristic peak, realizing the identification and differentiation of its homologues, and achieving the specific SERS detection of neomycin. Under the optimal experimental conditions, the linear detection range of neomycin is 10 ‑5 ~10 ‑10 M, and the relative standard deviation of SERS intensity is less than 10%. The method is simple, accurate and sensitive, and does not require complex operation technology, which meets the requirements of large-scale and rapid analysis and detection.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

Herein, an exploration was made of the potential therapeutic effect of injecting neomycin into the lung immediately after traumatic brain injury (TBI). It was hypothesized that modulation of the pulmonary microbiome may help alleviate neuroinflammation and cognitive impairment following TBI. Traumatic brain injury induced neutrophil infiltration into the alveoli along with interstitial pulmonary edema, and reduced levels of caspase-1, IL-1β, and RAGE. TBI rats treated with neomycin exhibited a significant decrease in Acinetobacter levels in BAL and a decrease in α-diversity Shannon index compared to rats without neomycin. The neomycin-administered TBI rats exhibited a higher discrimination index in a novel object recognition test, indicating that cognitive impairment was alleviated. While serum inflammatory cytokines in injured mice tended to increase for up to 7 days, such increases were not observed from day 3 in neomycin-administered TBI rats. Injection of neomycin into the lung immediately after TBI effectively alleviated neuroinflammation and cognitive impairment through modulation of the pulmonary microbiome.

The invention discloses a multi-immunochromatography test strip with unified detection limit for detecting four aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a colloidal gold pad, a sample pad and a PVC (polyvinyl chloride) bottom plate, wherein a gold-labeled antibody is fixed on the surface of the colloidal gold pad, and when a sample flows through the colloidal gold pad, the gold-labeled antibody is dissolved and serves as a tracer molecule to migrate along with a liquid phase; four detection areas T1, T2, T3 and T4 and a quality control line area C are arranged on the detection pad, a kanamycin antigen, a gentamicin antigen, a neomycin antigen and a spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse antibody is sprayed on the quality control line area C. According to the present invention, the expensive reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting kanamycin, gentamicin, neomycin and spectinomycin, and the detection limits can achieve 5 ng / mL.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.

The invention relates to an aptamer for specifically detecting neomycin and application of the aptamer, and belongs to the technical field of detection. Firstly, a novel aptamer (the affinity reaches 1.17 + / -0.32 mu M) with good affinity to neomycin is designed, and the aptamer has no reaction to other interfering antibiotics such as chloramphenicol (CAP), oxytetracycline (OTC), enrofloxacin (ENR), azithromycin (AZT), roxithromycin (RXM) and ciprofloxacin (CIP) and has good specificity. Secondly, two fluorescence signal probes of MOF coated FITC and AgNCs are also synthesized, a ratio type fluorescence sensing system is constructed, high-precision and high-sensitivity detection of the target neomycin is realized through a double-signal ratio, and background signal influence caused by environmental interference and fluorescent dye leakage is effectively eliminated.

The present application discloses a genetically engineered strain for producing neomycin B0, which is obtained from a mutant strain of the starting strain G. lozoyensis ATCC 74030 by modifying specific genes, and has an improved yield of neomycin B0 compared with the starting strain, wherein the genetic modification comprises overexpression of GLcs gene and overexpression of GLhyd gene, and preferably, the genetic modification further comprises one or more genetic modifications selected from the group consisting of knockout of GL6ma gene, knockout of GLpyrE gene, overexpression of GLp450-1 gene, overexpression of GLp450-2 gene, and overexpression of GLhyp gene. The present application also discloses a method for preparing the genetically engineered strain, and a method for preparing neomycin B0.

The invention provides a related medicine for treating senile diseases and application, the related medicine is used for inhibiting or removing methylmalonic acid in a body, and the related medicine is selected from at least one of coenzyme A, hydroxycobalamin, levocarnitine, betaine, carglutamic acid, metronidazole, neomycin, ACSF3 protein or an activator. According to the invention, the level of methylmalonic acid in vivo can be significantly reduced by using a removal technology (hemodialysis or application of a methylmalonic acid scavenger); after the methylmalonic acid is removed, the condition of senescence diseases can be obviously improved; mSC rejuvenation can be significantly increased by inhibiting methylmalonic acid.

The application discloses a method for increasing the number of early embryo implantation and promoting embryo development. The research result of the application shows that after superovulation, the endometrial receptivity is damaged, the embryo implantation site is increased, but the embryo development is slow, the sizes of the embryos are different, and the number of abnormal embryos is increased. The research of the application finds that neomycin can increase the number of embryo implantation after superovulation, and can significantly promote embryo development and reduce the number of abnormal embryos, thereby providing a new method for the production of livestock, poultry and pets.

The present invention relates to the field of medicine, in particular of oncology. Especially, it provides new compounds useful in the treatment of various cancers, such as glioblastoma, colorectal or breast cancers. The present disclosure also relates to pharmaceutical compositions containing the disclosed compounds.

The method comprises the following steps: adsorbing, washing and desorbing neomycin fermentation liquor through an adsorption system, and then concentrating to obtain desorbed concentrated liquor; the preparation method comprises the following steps: washing with low-concentration ammonia water, then dynamically adsorbing to cationic resin after dilution, washing with low-concentration ammonia water, then connecting weak positive resin and anion resin in series, washing with medium-concentration ammonia water, then desorbing with high-concentration ammonia water, and finally, concentrating, carrying out salt conversion and decoloration, and carrying out spray drying to obtain the Feramectin sulfate. According to the method, the conventional thought of primary adsorption and gradient washing of resin is broken through, weak positive and low-particle-size resin is selected for secondary dynamic adsorption and desorption, the better separation degree is achieved, the production cost is reduced, and the high-purity fralamectin sulfate is prepared.

This invention relates to the field of microbial detection technology and discloses a partitioned antimicrobial susceptibility testing plate and its application. The plate comprises a plate body and a plurality of microwell reaction units disposed on the plate body. The plate body is spatially divided into at least two independent detection zones, including a first detection zone and a second detection zone. A first selective culture system is pre-installed within the microwell reaction units of the first detection zone. The first selective culture system includes neomycin and at least one antimicrobial drug concentration gradient targeting Gram-negative bacilli. The partitioned antimicrobial susceptibility testing plate provided by this invention enables direct and simultaneous antimicrobial susceptibility testing of mixed samples of Gram-negative bacilli and Gram-positive cocci.

The present disclosure relates to a compound of Formula (I): (I), or a pharmaceutically acceptable salt or solvate thereof, where A, A1, A2, R, R1, R2, R3, R4, k, and m are as described above. The present disclosure also relates to a method of treating a fungal infection in a subject and a method of inhibiting the growth of yeast types of fungi or filamentous types of fungi using a compound of Formula (I) or a combination therapeutic comprising a compound of Formula (I) and an antifungal agent.

The invention belongs to the technical field of cell culture, and particularly relates to a black glue worm scavenger as well as a preparation method and a use method of the black glue worm scavenger. The concentration of the levofloxacin in the black glue worm scavenging agent is 25 to 50 [mu] g / ml; the mass ratio of the neomycin to the levofloxacin is (2: 1) to (1: 1). Compared with the prior art, the invention solves the problems of damage to normal cells, introduction of pollutants or byproducts, complex use method and long onset period of the black glue worm scavenger in the prior art. According to the black glue worm scavenger, the use mode and the onset period of the scavenger are simplified, and black glue worms and eggs of the black glue worms in cell culture can be efficiently removed under the condition that cells are not damaged.

The invention discloses a bacteriostatic composition for controlling staphylococcus aureus pollution and application thereof, and relates to the field of food safety, the bacteriostatic composition comprises a bacteriostatic part and a synergist; the antibacterial part is aminoglycoside antibiotics and / or disinfectants, and the synergist is 4-nitrobenzaldehyde. The 4-nitrobenzaldehyde provided by the invention can obviously enhance the bacteriostatic activity of various aminoglycoside antibiotics (such as gentamicin, kanamycin, tobramycin, neomycin and streptomycin) and common disinfectants (such as hydrogen peroxide, nisin and sodium hypochlorite) on staphylococcus aureus, and has broad-spectrum synergistic capacity.

The invention discloses a preparation method of an antibody for detecting ovalbumin and vancomycin in a biological product, the ovalbumin antibody is obtained by directly immunizing an animal, the vancomycin antibody is prepared by carrying out nucleophilic substitution reaction on vancomycin and N-(P-maleimidophenyl) isocyanate to obtain a vancomycin hapten, and the vancomycin hapten is used for detecting the ovalbumin and vancomycin in the biological product. The vancomycin immunogen is obtained by coupling the vancomycin with carrier protein, and then animals are immunized to obtain the vancomycin immunogen. The antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting ovalbumin contained in a biological product, the detection limit is 200 g / L; when the kit is used for detecting vancomycin contained in a biological product, the detection limit is 0.5 g / L. The detection result is high in accuracy, especially in a vancomycin antibody specificity experiment, the detection result shows that the detection result has remarkable specificity on vancomycin, and the detection result is not interfered by drugs such as polymyxin E, kanamycin, streptomycin, gentamicin, penicillin, tetracycline, chloramphenicol, neomycin, aureomycin, oxytetracycline and doxycycline.