235 results about "Neomycin" patented technology

The invention discloses a milk powder for calf, which comprises the following constituents (by weigh percent): whey powder 14-21%, whole milk powder 4-23.12%, puffed soybean 49-64%, concentrated soybean protein 1-3%, microelement premix 0.2-0.45%, vitamin premix 0.1-0.4%, calcium hydrogen phosphate 0.12-1.12%, table salt 0.03-0.06%, paunch accelerating agent 0.1-0.3%, intestinal tract protective agent 0.07-0.2%, neomycin 0.02-0.07%, terramicin 0.02-0.08%, anti-oxidizing agent 0.02-0.4%, lypressin 0.02-0.4%, hepionin 1-1.7%, tryptophan 0.5-0.75%, threonine 0.2-0.6%, stone powder 0.8-2% and fat 0.9-3%.

The invention discloses a medical composite alginate dressing containing antibacterial drugs and a preparation method thereof. In parts by weight, the medical composite alginate dressing comprises the following components: 50-99.9 parts of alginate and 0.05-10 parts of antibacterial drugs, The antibacterial drug is selected from gentamicin or its salt, neomycin or its salt, amikacin or its salt, fusidic acid or its salt, kanamycin or its salt, polycresol , mupirocin, sulfamethonium or its salt and clindamycin or its salt at least one. The medical composite alginate dressing of the present invention has strong ability to absorb exudate, has good water vapor transmission rate, can be biodegraded and has good biocompatibility, does not adhere to the wound and can quickly form gel after absorbing wound exudate , has strong moisturizing properties, can effectively promote wound healing, shorten wound repair time, can effectively prevent or treat sensitive bacterial infections, and can be widely used in acute and chronic wounds with exudate.

The invention discloses a lentiviral vector for preparation of CAR-T (chimeric antigen receptor T cells), a construction method of the lentiviral vector and an application of the lentiviral vector inanti-tumor cell product preparation. Meanwhile, the invention further provides a method for detecting virus titer with fluorescence quantitative PCR (polymerase chain reaction), and detection standardis provided for clinical application. The lentiviral vector is short, can stably and efficiently express a CAR gene in T cells and contains no genes such as GFP, Neomycin and the like irrelevant to treatment, and a high-titer lentivirus can be formed through packaging by the lentiviral vector in viral packaging cells such as HEK293T. Experiments prove that the lentiviral vector is suitable for research and clinical application of CAR-T.

The invention relates to a production method capable of increasing yield of neomycin. According to the production method, one or several of additives which are lysine, ferrous lactate, tyrosine, methionine, hydrolysis casein, vitamin B12, fructose, glucosamine, folic acid, riboflavin and tryptophan are added to a conventional fermentation medium comprising peanut meal, corn starch, rice flour, yeast powder, glucose, peptone, ammonium sulfate, sodium chloride, light calcium carbonate and KH2PO4-containign streptomyces fradiae. The production method has the advantages that the yield of the neomycin is increased by above 5-20%, the cost is reduced, and the fermentation efficiency is high. The neomycin is used for treating human diseases, widely used in animal husbandries and used as antibiotics.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol/m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.

This invention is for an improved process to co-encapsulate hydrophobic drugs and hydrophilic drugs in phospholipid liposomes. Non-toxic supercritical or near-critical fluids with/without polar cosolvents are utilized to solubilize phospholipid materials and hydrophobic drugs, and form uniform liposomes to encapsulate hydrophobic drugs and hydrophilic drugs.

DNA topoisomerase I (Top1) is the target of camptothecin, and novel Top1 inhibitors are in development as anticancer agents. Top1 inhibitors damage DNA by trapping covalent complexes between the Top1 catalytic tyrosine and the 3′-end of the broken DNA. Tyrosyl-DNA phosphodiesterase (Tdp1) can repair Top1-DNA covalent complexes by hydrolyzing the tyrosyl-DNA bond. Inhibiting Tdp1 has the potential to enhance the anticancer activity of Top1 inhibitors and to act as antiproliferative agents. It has been recently reported that neomycin inhibits Tdp1 more effectively than the related aminoglycosides paromomycin and lividomycin A. Inhibition of Tdp1 by neomycin is observed both with single- and double-stranded substrates but is slightly stronger with duplex DNA, which is different from aclarubicin, which only inhibits Tdp1 with the double-stranded substrate. Inhibition by neomycin can be overcome with excess Tdp1 and is greatest at low pH. Aminoglycoside antibiotics and the ribosome inhibitors thiostrepton, clindamycin-2-phosphate, and puromycin are the first reported pharmacological Tdp1 inhibitors. The development of Tdp1 inhibitors as anticancer agents can be envisioned as combinations of Tdp1 and Top1 inhibitors. Moreover, Tdp1 inhibitors might also be effective by themselves as anticancer agents. In addition, Tdp1 inhibitors might be valuable as anti-infectious agents.

This invention can produce a co-encapsulated combination drug product consisting of a topoisomerase 1 inhibitor such as camptothecins including neat camptothecin and its derivatives irinotecan, topotecan and other derivatives, and a tyrosyl-DNA phosphodiesterase (Tdp1) such as aminoglycoside antibiotics including neomycin and tetracycline, and the ribosome inhibitors thiostrepton, clindamycin-2-phosphate, and puromycin.

The invention discloses a specific monoclonal antibody capable of identifying neomycin, amikacin and paromomycin and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting neomycin, amikacin and paromomycin. The monoclonal antibody is secreted by a hybridoma cell EDC/5G04 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201144. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples and the like. Compared with the prior art, the monoclonal antibody, the ELISA method and the kit have the following main advantages that the prepared monoclonal antibody can simultaneously identify neomycin, amikacin and paromomycin, thus improving the detection efficiency of the prior art; the animal tissue sample treatment method is simple and short in time; and the ELISA method and the kit also have the characteristics of high detection sensitivity, good precision and good accuracy.

The invention discloses a molecular engram integral cylinder and its process method for detecting gentamicin, streptomycin and neomycin residue in animal food.The method adopts gentamicin, streptomycin and neomycin as molecular modular and combining in original position to process gentamicin, streptomycin and neomycin molecular engram integral cylinder. The stuff in cylinder is a crosslinked polymers of determined range functional group with fixed hole size and shape, which has memory function for modular molecular spatial structure of gentamicin, streptomycin and neomycin.

The invention relates to the microbial field, and discloses corynebacterium glutamicum strains and application thereof. A corynebacterium glutamicum MHZ-0510 strain has a preservation number CGMCC No.7939; a corynebacterium glutamicum MHZ-0511 strain has a preservation number CGMCC No.7940; the two strains have significantly improved ability of fermentation for generating L-glutamine, compared with that of the original strain of wild type of corynebacterium glutamicum B498, and can be widely applied to fermentation production of L-glutamine. The corynebacterium glutamicum strains involved in the invention are obtained by screening aminoglycoside antibiotics, such as streptomycin and neomycin, have resistance to the aminoglycoside antibiotics including streptomycin and neomycin, and have improved ability to synthesis of L-glutamine, thus increasing the yield of L-glutamine. Therefore, aminoglycoside antibiotics can be widely applied to screen of L-glutamine.

The invention relates to an enzyme-linked immunodetection method of aminoglycoside antibiotics in animal-derived foods, belonging to the immnunoassay field. The invention utilizes a carbodiimide method to synthesize immunogen (NM)L-(KM)m-(GM)n-(SM)k-BSA with multiple antigen determinants, such as aminoglycoside antibiotics, by four steps, utilizes a glutaric dialdehyde method to synthesize the envelope antigen of the aminoglycoside antibiotics and establish the enzyme-linked immunodetection method of the aminoglycoside antibiotics in the animal-derived foods; a polyclonal antibody with the specificity of aminoglycoside cluster is adopted as a detection reagent; gentamicin, neomycin, kanamycin or streptomycin is adopted as a standard substance, the four antibiotics and OVA conjugate are adopted as the envelope antigens, and the establishment of an ELISA method for detecting multi-residue of the aminoglycoside antibiotics provides a detecting means with high speed and high efficiency for the multi-residue detection of the aminoglycoside antibiotics in the animal-derived foods. The invention has the advantages of simple pre-processing, high sensitivity, high precision and the like.

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

The invention discloses a neomycin semi-quantitative gold-labeled rapid detection kit, and a detection method and an application thereof, and belongs to the field of immunology. The kit includes a sample diluent, an enzyme label plate and neomycin gold-labeled rapid detection strips; each detection strip is composed of a sample pad, a gold-labeled antibody combination pad, a nitrocellulose film and a water absorption pad; the gold-labeled antibody combination pad is a neomycin monoclonal antibody labeled by colloidal gold; two detection lines and a quality-control line are coated at the middle part of the nitrocellulose film; a reaction of the neomycin antibody and an neomycin antigen is showed in a colored reaction mode through a colloidal gold immunochromatography technology, and during the detection process, whether neomycin residues exist and a residual amount range in dairy products are determined through changes of colors of the two detection lines. The detection kit can achieve semi-quantitative detection of the residues, has the advantages of simple detection method, short detection time, low detection cost, wide detection range, accurate and reliable detection results, and can be widely applied to neomycin semi-quantitative detection in food.

The invention aims to provide an animal semen preservative with biological activity and a preparation method of the animal semen preservative. The animal semen preservative with the biological activity contains oligoprocyanidine so that the animal semen preservative has an anti-oxidation effect, can prolong the preservation time of semen and keeps the activity of animal sperms. The animal semen preservative is prepared by mixing 1000mL of a common medium and 16mg of oligoprocyanidine; the common medium is prepared by mixing 6 parts of glucose, 0.37 part of EDTA (Ethylene Diamine Tetraacetic Acid), 0.375 part of sodium citrate dihydrate, 0-0.12 part of sodium bicarbonate, 0.1 part of neomycin and 100 parts of distilled water; the neomycin is independently added before the semen is diluted. The formula contains the oligoprocyanidine so that the animal semen preservative has the anti-oxidation effect and can prolong the preservation time of the semen; the degradation of a semen sample can be relieved by the oligoprocyanidine and the oligoprocyanidine has no negative effects on the activity of the sperms, so that the activity of the animal sperms is kept; particularly, the survival rate of the sperms preserved at a low temperature is obviously improved.

The invention discloses a time resloved fluoroimmunoassay (TRF) kit for detecting Galectin-3.The kit comprises: a coating buffer, a blocking solution, a cleaning solution, a loading buffer, a enhancement solution, a assay buffer, coated antibody (monoclonal anti-human Galectin-3 antibody), detection antibody (biotinylated anti-human Galectin-3 antibody), Eu<3+> labeled streptavidin and neomycin, a standard substance (recombinant human Galectin-3). The kit is characterized in that: the kit adopts a double antibody sandwich method and TRF principle for detecting a concentration of the Galectin-3 in a sample. The method is characterized by: coating an ELISA plate through the coated antibody; adding the sample to be detected to the ELISA plate to carry out a incubation after blocking; washing the plate, then adding the detection antibody to the plate to carry out the incubation; washing the plate again, followed by adding the Eu<3+> labeled streptavidin to carry out the incubation; washing the plate, followed by adding the enhancement solution; oscillating for 5 minutes, followed by exciting through light having a excitation wavelength of 337nm; detecting a fluorescence value of the sample at a emission wavelength of 615nm after delaying 200 microseconds; drawing a standard curve through the concentration of the standard substance and the corresponding fluorescence value, such that the concentration of the Galectin-3 in the sample to be detected can be calculated through the fluorescence value and the standard curve.

The invention discloses a method for producing neomycin by replacing part of fermenting raw materials with soybean meal. The method consists of the following steps: (1) culturing bevel spores; (2) culturing a bevel spore suspension; (3) performing seeding tank culturing: putting the bevel spore suspension into a seed culture tank for culturing; (4) performing fermentation tank culturing: feeding a culture solution subjected to the seed tank culturing into a fermentation culture tank for culturing, wherein the culture solutions in the seed tank and the fermentation tank include soybean meal of which the mass content is 2.0-5.0 percent the mass of the culture solution in the fermentation tank, and the culture solution in the fermentation tank does not contain peptone, yeast powder and peanut powder; (5) terminating fermentation, diluting a fermentation solution, neutralizing till the pH value becomes 6.4-7.0 to obtain a diluted fermentation solution, thereby finishing the fermentation step. According to the method for producing the neomycin by replacing part of the fermenting raw materials with the soybean meal, the problems of complex raw material formula and high cost of the culture solution are solved, the formula is simplified effectively, the cost is reduced, and the production of the neomycin is guaranteed.

The invention relates to a culture medium for producing neomycin by utilizing fermentation of streptomyces fradiae. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein soy peptone, biological fish meal, millet meal, zinc lactate, ammonium molybdate and other substances are added into the culture medium, so that metabolism of strains can be benefited, fermentation starting titer can be improved, the aging speed of hyphae can be delayed, the neomycin production capability can be improved, the fermentation cycle can be shortened, the fermentation cost can be reduced, and high-efficiency production of neomycin can be realized.

An expression carrier for effectively screening target protein is prepared through introducing an internal ribosome to the eukaryotic expression carrier containing CMV promoter and dhfr amplification system, linking the fusion gene of anti-erbB2-seFv-Fc gene and IL-2 to the neomycin-resistant gene, site mutation to Neo gene by PCR, and configuring the expression carrier pCMV-HFI-mNeo.