The invention discloses a multi-immunochromatography test strip with unified
detection limit for detecting four
aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a
colloidal gold pad, a sample pad and a PVC (
polyvinyl chloride) bottom plate, wherein a gold-labeled
antibody is fixed on the surface of the
colloidal gold pad, and when a sample flows through the
colloidal gold pad, the gold-labeled
antibody is dissolved and serves as a tracer molecule to migrate along with a
liquid phase; four detection areas T1, T2, T3 and T4 and a
quality control line area C are arranged on the detection pad, a
kanamycin antigen, a
gentamicin antigen, a
neomycin antigen and a
spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse
antibody is sprayed on the
quality control line area C. According to the present invention, the expensive
reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting
kanamycin,
gentamicin,
neomycin and
spectinomycin, and the detection limits can achieve 5 ng / mL.