3 results about "Neomycin" patented technology

The present application belongs to the technical field of veterinary drug residue detection, and particularly relates to a detection method of neomycin and apramycin in veterinary drugs, wherein a hydroxylated silver nanowire dispersion liquid and a dopamine modified Ag NCs@TiO2 nanoparticle dispersion liquid are mixed in equal volume, then embedded in a PDMS flexible substrate material to obtain a flexible SERS substrate material, a spiked sample solution is dropped onto the surface of the flexible SERS substrate material, and then dried and subjected to SERS detection, a linear regression equation is fitted by taking the characteristic peak intensity of the antibiotic and the logarithmic value of the concentration of the spiked sample solution as variables, a sample solution to be detected is dropped onto the surface of the flexible SERS substrate material, and then subjected to SERS detection to calculate the residual concentration of the antibiotic in the sample solution to be detected. The present application adopts a surface enhanced Raman scattering technology (SERS) to detect the residual amounts of neomycin and apramycin in meat products, and the detection is faster and simpler.

The present application discloses a genetically engineered strain for producing neomycin B0, which is obtained from a mutant strain of the starting strain G. lozoyensis ATCC 74030 by modifying specific genes, and has an improved yield of neomycin B0 compared with the starting strain, wherein the genetic modification comprises overexpression of GLcs gene and overexpression of GLhyd gene, and preferably, the genetic modification further comprises one or more genetic modifications selected from the group consisting of knockout of GL6ma gene, knockout of GLpyrE gene, overexpression of GLp450-1 gene, overexpression of GLp450-2 gene, and overexpression of GLhyp gene. The present application also discloses a method for preparing the genetically engineered strain, and a method for preparing neomycin B0.

This invention relates to the field of microbial detection technology and discloses a partitioned antimicrobial susceptibility testing plate and its application. The plate comprises a plate body and a plurality of microwell reaction units disposed on the plate body. The plate body is spatially divided into at least two independent detection zones, including a first detection zone and a second detection zone. A first selective culture system is pre-installed within the microwell reaction units of the first detection zone. The first selective culture system includes neomycin and at least one antimicrobial drug concentration gradient targeting Gram-negative bacilli. The partitioned antimicrobial susceptibility testing plate provided by this invention enables direct and simultaneous antimicrobial susceptibility testing of mixed samples of Gram-negative bacilli and Gram-positive cocci.