12 results about "Susceptibility testing" patented technology

The invention discloses a high-precision identification method for a culture medium drug sensitive paper sheet based on a mixed model, and the method comprises the steps: S1, image collection and preprocessing: carrying out the standardization processing of a collected culture medium image, including noise removal, illumination correction and contrast enhancement; s2, semantic segmentation model processing: carrying out pixel-level classification on the preprocessed image by adopting a deep learning network architecture, accurately positioning the positions and boundaries of all drug sensitive paper sheets in a culture medium, and generating a segmentation mask; and S3, classification model processing: extracting a paper sheet area based on a result of the step S2, and identifying a drug code and concentration on each paper sheet by using a CNN classification model. And S4, hybrid model fusion: intelligently fusing the data in the steps S2 and S3, and outputting a final drug sensitive paper identification result through confidence weighting and result verification. According to the method, semantic segmentation and classification models are fused, the difficulties of low recognition precision, poor environmental adaptability and the like of a traditional drug sensitive test are overcome, and high-precision automatic recognition of the drug sensitive paper sheets is realized.

The invention belongs to the field of biological medicine, and particularly relates to a mass spectrum tag probe for a rapid drug sensitive test as well as preparation and application thereof, and the mass spectrum tag probe mainly comprises three parts, namely a gold nanoparticle carrier, a pegylated antibiotic target head and a mass spectrum tag peptide fragment. The probe disclosed by the invention is low in preparation cost, good in stability and high in detection speed (only 4 hours), and compared with a traditional drug sensitive test, the probe disclosed by the invention can be used for directly detecting a complex bacterial sample and does not need to be purified and cultured; compared with biosensing and mass spectrometry technologies, the probe provided by the invention has higher detection sensitivity and is not easily influenced by a matrix; compared with a qPCR technology, the method does not need to know the sequence information of the drug-resistant gene in advance, can directly detect the apparent sensitivity / drug resistance of bacteria, and is wider in detection range; compared with biosensing, mass spectrometry, qPCR and other methods, the probe provided by the invention can realize detection of various pathogenic bacteria and detection of sensitivity of multiple antibiotic drugs.

The utility model discloses a metal hot crack sensitivity detection device, relates to the technical field of metal casting, and aims to solve the problem of lack of a metal hot crack sensitivity detection device for molten metal in the prior art. The casting cavity is communicated with a sprue, ring iron is arranged in the casting cavity, the ring iron and the casting cavity are concentrically arranged, the width of the ring iron is the same as that of the casting cavity, and the diameter of the ring iron is smaller than that of the casting cavity; a chilling block is arranged at the bottom of the casting cavity, and the upper surface of the chilling block and the bottom face of the casting cavity are arranged at the same height.

The utility model provides a kit for rapidly identifying and culturing drug sensitivity of salmonella, and relates to the field of salmonella culture. The salmonella rapid identification culture drug sensitivity kit comprises a kit body and a kit cover, a container used for culturing salmonella is fixedly installed in the kit body, a base plate is fixedly installed on the inner bottom wall of the container, a telescopic rod used for rotating is fixedly connected to the base plate, a filter screen and an arc-shaped plate are fixedly installed at the movable end of the telescopic rod, and the arc-shaped plate is fixedly connected with the filter screen. The filter screen is slidably connected with the container; the rapid identification and culture drug sensitivity kit for salmonella integrates sample separation, dynamic culture and drug sensitivity test, after culture is completed, a drug sensitivity reagent can be directly added for the drug sensitivity test, sample transfer is not needed, operation steps and time are reduced, rapid identification and drug sensitivity detection of salmonella are achieved, and the kit is suitable for large-scale popularization and application. And the detection efficiency and accuracy are improved.

A drug susceptibility testing device is provided. [Solution] Before starting the test device 1 and conducting the test, the incubation unit 3 is irradiated using a growth-identifying light source 4, and the light source detection module 41 receives growth information obtained from the original sample volume after irradiation to determine whether incubation is complete. The sample is diluted and placed in the reaction chamber of the reagent disk plate 2, and the entire plate is placed in the detection installation slot 11 to perform the test. After the reaction, the same reaction chamber is sequentially irradiated with a first detection light source 51 and a second detection light source 52, each with different wavelengths. Finally, the color information obtained from the AST-specific indicator is converted into wavelength information by the spectral processing module, resulting in the detection result. The sample growth status and detection results can be accurately determined in a simple and quick manner. The detection results are analyzed using two inspection light sources and the spectral processing module, eliminating manual judgment and allowing highly accurate results to be obtained by comparing them with each other.

Methods for identifying bacterial species in biofluid samples (e.g., whole blood samples) are described. The methods rely on optical spectroscopy, and enable rapid detection and identification of bacteria directly from whole blood. Not only can LSS-based techniques detect and identify bacteria in biofluids such as whole blood, but that species-level identification can potentially be made based on a small number of bacterial cells, without the need for observing entire colonies or performing susceptibility testing. The methods may comprise illuminating the biofluid sample with input light, detecting scattered light produced by the biofluid sample in response to the illuminating, generating first data indicative of a measured scattering spectrum associated with the biofluid sample using the detected scattered light, and identifying whether at least one of the bacterial species is present in the biofluid sample using the first data.

The invention relates to the technical field of data processing, and discloses a combined drug sensitive test data processing method, device and equipment and a storage medium, and the method comprises the following steps: obtaining a combined drug sensitive plate optical density value matrix output by a microplate reader and a set growth inhibition threshold; based on the optical density value matrix of the combined drug sensitive plate and the growth inhibition threshold, respectively judging the growth state corresponding to each hole in the combined drug sensitive plate; calculating a corresponding partial bacteriostatic concentration index according to the hole with the growth state in growth inhibition; and determining the combined effect of the two drugs based on the calculated minimum partial bacteriostatic concentration index. According to the invention, the accuracy and reliability of the combined drug sensitive test result can be improved.

A sealing microlid (novel microplate sealing lid) designed to evaluate the biological effects of volatile agents in vapour phase in combination with a microplate is disclosed. The sealing microlid includes a plate having a surface, a plurality of flanges extending from the surface, and a protruding opening tab. The flanges of the sealing microlid serve as reservoirs for growth medium containing organisms or cells, which will grow on it, and, at the same time, generate an airtight condition with the well of the microplate necessary for leakage-prevention of the volatile agents tested. The apparatus of the present invention allows quantitative, rapid, simple, labour-and cost-effective susceptibility testing of organism or cell cultures to volatile agents in vapour phase.

The invention provides a bacterial drug resistance prediction method and system based on a time sequence electronic health record, and relates to the technical field of drug resistance prediction, and the method comprises the steps: extracting patient features from the electronic health record; performing two-dimensional and three-dimensional feature extraction on the drug molecules to obtain drug features; extracting bacterial characteristics according to the bacterial genome sequence; extracting drug sensitivity characteristics according to a drug sensitivity test of microorganisms; respectively aligning other features with the drug sensitive features; initializing a modal shared space, fusing the drug sensitive test time difference into a query vector, and calculating the attention of the shared space to each modal feature; aggregating all the features into a shared space according to the attention to obtain comprehensive features of the drug sensitive test; and inputting the comprehensive features into a pre-constructed prediction model to obtain a prediction result. According to the method, the problems that time features are not utilized in drug resistance early warning, interaction between patient features is ignored, and modeling of drug molecules and bacteria is lacked are solved, and the accuracy of drug resistance prediction is improved.

To provide a structure of a reagent disk plate in a drug susceptibility test that simplifies the procedure for advance preparation of the test, distributes specimens uniformly and appropriately, prevents cross-infection of specimens, and prevents damage and contamination of the medium due to excessive gas. The device comprises a test medium 1, at least two support restriction sections 112, a central injection section 2, multiple microchannels 3, multiple reaction channels 4, multiple preset reagents 41 specifically for AST, multiple overflow channels 5, multiple overflow channels 6, multiple converging sections 51, and multiple exhaust holes 7. Before the test medium is sealed, freeze-dried preset reagents are placed in the reaction channels. When the user injects a sample into the central injection section, the test medium is placed in the testing device. Upon activation, the sample flows uniformly from the microchannels to the reaction channels under the action of centrifugal force. If the sample content in the reaction channel exceeds its capacity, the sample flows into the overflow channel due to the converging section of the overflow channel, and gas generated during the reaction is discharged through the exhaust hole.

Methods are presented which use impedance flow cytometry for rapid susceptibility testing of antimicrobial agents including phage, antimicrobial peptides and rapid analysis of antimicrobial mediated serum bactericidal assays.

This invention discloses a pan-drug-resistant strain of *Pseudomonas aeruginosa* PAS96 and its applications. *Pseudomonas aeruginosa* PAS96 has the accession number GDMCC No: 64767. This bacterium is resistant to levofloxacin, ciprofloxacin, piperacillin, piperacillin / tazobactam, ticarcillin / clavulanic acid, ceftazidime, meropenem, and imipenem. This invention confirmed the resistance of *Pseudomonas aeruginosa* to the aforementioned antibiotics through Kirby-Bauer (K-B) susceptibility testing.