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Automatic detection method for mycoplasma culture, identification, counting and drug sensitivity results

A technology for automatic detection and mycoplasma, applied in biochemical equipment and methods, methods based on microorganisms, measurement/inspection of microorganisms, etc., can solve problems such as inaccurate errors, a large amount of manpower input, strong subjectivity, etc., and achieve accurate analysis and processing, The effect of liberating manpower and time investment

Inactive Publication Date: 2016-02-03
ZHEJIANG UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0004] At present, mycoplasma culture, identification, counting and drug susceptibility testing in microbiology laboratories are generally carried out using kits (colorimetric method). The reading, sorting and analysis of the results requires a lot of manpower input, and not only cannot achieve rapid automation of the results Read and analyze, and the original picture information of the results cannot be saved in the clinical laboratory information system (LIS) for result query and verification. At the same time, errors will inevitably occur when manually reading and analyzing large batches of results and cannot be stored in the computer LIS system Find out in time through review during audit
To sum up, the current results of mycoplasma culture, identification, counting, and drug susceptibility testing are time-consuming and labor-intensive to read and analyze, subjectivity is strong, errors are large, automatic analysis and processing have not been achieved, and the original pictures of the results have not been realized in the computer system auto save

Method used

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  • Automatic detection method for mycoplasma culture, identification, counting and drug sensitivity results
  • Automatic detection method for mycoplasma culture, identification, counting and drug sensitivity results
  • Automatic detection method for mycoplasma culture, identification, counting and drug sensitivity results

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Embodiment Construction

[0016] The present invention is illustrated by taking Mérieux as an example (the application of the present invention is not limited to related products of this company). According to Mérieux Diagnostic Products (Shanghai) Co., Ltd. Mycoplasma Culture, Identification, Counting and Drug Sensitivity Test Kit (colorimetric method) instruction manual, the test result judgment standard is as follows: Table 1:

[0017]

[0018] Note: In the table, Uu stands for Ureaplasma, Mh stands for Mycoplasma hominis, doxycycline is abbreviated as DOT, josamycin is abbreviated as JOS, ofloxacin is abbreviated as OFL, erythromycin is abbreviated as ERY, and tetracycline is abbreviated as TET, ciprofloxacin is abbreviated as CIP, azithromycin is abbreviated as AZI, clarithromycin is abbreviated as CLA, and protomycin is abbreviated as PRI. The numbers below antibiotics represent low and high concentrations, respectively, in milligrams per liter. After 24 hours, read only reaction cup 4 (Uu≥10 4 ), ...

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Abstract

The invention discloses an automatic detection method for mycoplasma culture, identification, counting and drug sensitivity results. The method comprises the following steps: based on a visual image acquisition system and corresponding automatic analysis software, converting an optical signal of an image into a digital signal by using the visual image acquisition system; displaying images of mycoplasma culture, identification, counting and drug sensitivity results on a computer screen; allowing the automatic analysis software to automatically analyze permutation and combination of different colors according to preset conditions with the colors of the images and color collocation and combination as indexes; and displaying qualitative identification results of ureaplasma (Uu) and / or Mycoplasma hominis (Mh), semi-quantitative counting results of Uu and / or Mh and drug sensitive test results of antibiotics. The method provided by the invention can overcome problems in automatic monitoring of mycoplasma culture, identification, counting and drug sensitivity results, improves automatic storage of original pictorial information so as to facilitate retrospective query and check of an original result database and can rapidly, timely and accurately analyze and process large-batch original result data.

Description

Technical field [0001] The invention relates to a commonly used technology for collecting, transmitting, reading and storing picture information of mycoplasma culture, identification, counting and drug sensitivity results, and a system for intelligently analyzing the results of different combinations, which can be applied to the hospital microbiology room Automated analysis and report of mycoplasma culture, identification, counting and drug sensitivity results. Background technique [0002] Mycoplasma is a type of prokaryotic microorganism that lacks cell walls. The main mycoplasmas related to human diseases are Ureaplasma Urealyticum (Uu), Mycoplasma Hominis (Mh) and Mycoplasma Pneumoniae (Mp), among which Uu and Mh are internationally recognized causes The pathogen of genitourinary tract infection has the highest incidence rate among sexually transmitted diseases. Both Uu and Mh are pathogens parasitizing the genitourinary tract and often cause genitourinary tract diseases. No...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/18C12R1/35
Inventor 叶光勇朱宇宁梁宇
Owner ZHEJIANG UNIV
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