59 results about "Erythromycin" patented technology

The present invention discloses a method for simultaneously reducing the biological inhibition of antibiotics and thiocyanate in erythromycin wastewater, and relates to the technical field of industrial wastewater treatment. The specific treatment method includes: by regulating the water quality characteristics of the wastewater, adding a selective reaction reagent, and regulating the reaction temperature, time, and stirring rate, the simultaneous microbial toxicity reduction of antibiotics and thiocyanate in the erythromycin wastewater is achieved. The method of the present invention has a stable reaction process, easy-to-regulate process conditions, a short time consumption, a strong selectivity in the removal process, and no need to regulate or remove other substances in the wastewater; it effectively solves the problem that erythromycin wastewater has a strong inhibitory effect on anaerobic biological processes due to the presence of high concentrations of erythromycin residual titers and thiocyanate ions, making it difficult to enter the anaerobic biological treatment process for efficient organic matter removal, and has good industrial application prospects.

The invention discloses an efficient detoxification and nutrient recovery method for aerobic composting of fungus-enhanced erythromycin fermentation fungus residues, and belongs to the field of solid waste disposal and resource reutilization. The method comprises the following steps: mixing erythromycin fermentation mushroom dregs subjected to high-temperature sterilization treatment with corn straw powder, inoculating trichoderma asperellum, and carrying out aerobic composting fermentation. According to the method, aerobic co-compost of erythromycin fermentation mushroom dregs and crop straws is strengthened by inoculating an antibiotic degrading microbial agent taking trichoderma asperellum as a core, and an organic fertilizer which is high in nutrient content and is non-toxic and harmless is produced, so that an efficient, economical and environment-friendly solution is provided for solving the problem of resource utilization of the erythromycin fermentation mushroom dregs.

The invention discloses a method for preparing azithromycin by using a microchannel reactor. The method uses erythromycin as a starting material and prepares azithromycin through oximation, rearrangement, reduction and methylation reactions. During the oximation reaction, the contact time of the feed liquid in the microchannel reactor is very short, which greatly reduces the safety risk of the oximation reaction. The reduction reaction is carried out in the microchannel reactor, and neither explosive sodium (potassium) borohydride nor a high-pressure reactor is used, which reduces the safety risk. A tray layer of palladium carbon is laid on the reaction bed to achieve the hydrogenation target, while greatly improving the utilization efficiency of palladium carbon. In the rearrangement reaction, a milder leaving group, sodium 2-naphthalenesulfonate, is used to replace the existing leaving group. Water is used as a solvent and the reaction is carried out at room temperature. The method is green, safe, and has a high yield, and is suitable for industrial production.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The invention provides a staphylococcus aureus drug resistance prediction method based on whole genome association analysis and machine learning, and belongs to the technical field of bioinformatics and artificial intelligence crossing. Five common antibiotics such as erythromycin, ciprofloxacin, penicillin, fusidic acid and methicillin are selected as modeling objects. Performing comparison and SNP site detection on a strain genome by using a reference genome, and constructing a variation matrix; then, a pyseer tool is adopted for GWAS analysis, and genetic loci significantly related to the drug resistance of each antibiotic are screened as modeling features; and for each antibiotic, respectively constructing an independent machine learning model, inputting the SNP features screened by the GWAS, and outputting a drug resistance / sensitivity classification label. Through cross validation and independent test set evaluation, the model has excellent accuracy and F1 score on multiple antibiotics. According to the method, drug resistance prediction can be achieved based on genome data without traditional drug sensitivity experiments, and the method has the remarkable time advantage, popularization value and scientific research significance.

This invention discloses a compound ointment for protecting the nasal mucosa and repairing skin damage, and its preparation method, belonging to the field of pharmaceutical technology. The compound ointment is formulated with Sophora japonica fruit extract, erythromycin ointment, vitamin B6, levofloxacin hydrochloride tablets, loratadine tablets, fluticasone propionate nasal spray, sesame oil, and honey in specific weight proportions. The components work synergistically, providing protection for the nasal mucosa, treatment of rhinitis, reduction of cold air and external pollutant intrusion, and prevention of colds. It can also treat hemorrhoids, scars, eczema, and other skin damage, achieving "one medicine for multiple uses." This compound ointment has a stable dosage form, is convenient to use, has high utilization rate, high safety, and no obvious side effects, making it suitable for various populations and possessing good industrialization prospects.

The invention discloses D-type mannosylerythritol lipid as well as a preparation method and application thereof. According to the invention, the green and bio-friendly D-type mannosylerythritol lipid is used as a biological bacteriostatic agent and is independently used or combined with other bacteriostatic agents (cefaclor, gentamicin or erythromycin and the like); effective inhibition and even killing of various pathogenic microorganisms (methicillin-resistant staphylococcus aureus, bacillus subtilis or bacillus cereus and the like) are realized. The novel bacteriostatic strategy of the D-type mannosylerythritol lipid provided by the invention not only can effectively realize efficient bacteriostasis, reduce the dosage of an antibacterial agent and reduce the graded bacteriostatic concentration index to 0.09, but also shows good biocompatibility, has a therapeutic index as high as 44.81, and has a wide application prospect.

The application provides a multi-layer dry heat sterilization device for erythromycin eye ointment production, and belongs to the technical field of dry heat sterilization cabinets. The device comprises a dry heat sterilization cabinet, a shelf is arranged in the dry heat sterilization cabinet, a tray base is arranged on the shelf, a plurality of first avoiding grooves are symmetrically arranged on the outer walls of the two sides of the tray base, first guide plates are symmetrically and fixedly connected to the inner walls of the two sides of the tray base close to the top, connecting columns are fixedly connected to the outer walls of the two sides of the tray base close to the first avoiding grooves, first handles are fixedly connected to the ends of the connecting columns away from the tray base, an auxiliary assembly is arranged on the tray base, and the auxiliary assembly is used for improving the temperature rising speed of the aluminum pipe. The auxiliary assembly can shorten the temperature rising time of the aluminum pipe of the eye ointment and reduce the operation energy consumption of the dry heat sterilization cabinet when the dry heat sterilization cabinet works.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

This invention relates to the field of industrial process automation control and waste liquid resource utilization technology, specifically to a concentration regulation system for preparing liquid fertilizer from erythromycin waste liquid based on process control. The system includes: a data acquisition terminal acquiring fluid dynamics time-series data, concentration characterization time-series data, and actuator status data; an ideal reference reconstruction module reconstructing ideal concentration data based on the fluid dynamics time-series data and actuator status data; an interference simulation module injecting interference into the ideal concentration data based on preset fouling characteristics, rheological interference characteristics, and fluid dynamics time-series data to generate simulated concentration data; a coupling decision module generating actual concentration estimation data based on the concentration characterization time-series data, and accordingly generating regulation commands including closed-loop regulation, freeze regulation, cleaning, and temperature compensation commands; and a regulation execution module responding to the regulation commands and controlling the corresponding mechanisms. This invention ensures that the actions do not excessively rely on pollution observation values.

The utility model discloses a kind of erythromycin zinc acetate ointment production stirring devices, including support frame, stirring mechanism, dustproof mechanism and heating mechanism, the stirring mechanism includes stirring barrel, first stirring device, second stirring device and connecting assembly, the stirring barrel is set on support frame upper end face, the inlet pipe is provided on the upper end surface of the stirring barrel, the first stirring device includes first motor, main shaft and first stirring blade, the first motor passes through stirring barrel and is connected with main shaft, the first stirring blade is fixedly connected with main shaft, the second stirring device includes shaft, second stirring blade, large gear and pinion, the shaft is rotatably connected with connecting assembly, the large gear and the pinion are arranged in the inside of stirring barrel, one end of the shaft is fixedly connected with pinion, the large gear is fixedly connected with the upper of the inner wall of stirring barrel, the large gear and pinion are engaged, this stirring mechanism significantly improves mixing efficiency and mixing homogeneity.

The invention relates to application of an activating agent in promotion of uranium element discharge. Specifically, the activating agent is prepared from rifampicin, erythromycin, progesterone, aldosterone, bilirubin, cholate vinblastine, doxomicin, adriamycin, paclitaxel, bosentan, ambrisentan, digoxin, verapamil, tonavir, amprenavir, indinavir, hypericum perforatum juice, curcumin, atorvastatin beclomethasone, budesonide, divaricasone carbamazepine and caffeine. And phenytoin. The use dosage of the activating agent is 10 to 60 mg / kg. According to the method, the accumulation amount of uranium in important organs such as bones and kidneys can be remarkably reduced, so that the potential toxic influence on the key organs is reduced.

Disclosed herein are new salts and polymorphs of cethromycin for the treatment of diseases due to infection by bacteria and certain protozoans, including, for example, malaria, Babesosis, Toxoplasmosis, diarrheal disease, respiratory disease, sexually transmitted bacterial infections, and some bioterror bacteria, including, for example, plague, tularemia and post-inhalation anthrax. Also disclosed herein are new salts and polymorphs of cethromycin for the treatment of inflammatory diseases, including, for example, pelvic inflammatory disease, and peptic ulcer disease.

The invention discloses a bacillus amyloliquefaciens genetically engineered bacterium with high antibiotic resistance as well as a construction method and application thereof, the construction of the engineered bacterium comprises the following steps: bacillus amyloliquefaciens is taken as a starting strain, an RNA (Ribonucleic Acid) molecular chaperonin hfq coding gene is knocked out, and the nucleotide sequence of the RNA molecular chaperonin hfq is shown as SEQ ID NO.1. The RNA molecular chaperonin hfq is obtained through cloning, the tolerance of engineering bacteria to erythromycin and ampicillin is increased by 2.44 times and 3.79 times by knocking out the gene or reducing the expression of the gene, and the enhancement of the antibiotic resistance of bacillus is crucial to the threat of drug-resistant bacteria, the improvement of environmental adaptability and the development of biotechnology application. By strengthening the resistance gene, drug-resistant pathogens can be efficiently inhibited, the antibacterial curative effect can be improved, the metabolic activity can be maintained in a complex antibiotic environment, and the pollution repair and industrial fermentation efficiency can be guaranteed.

The invention discloses an MphR protein mutant and an erythromycin whole-cell biosensor constructed based on the MphR protein mutant. The amino acid sequence of the MphR protein mutant is as shown in SEQ ID NO: 5, SEQ ID NO: 7 or SEQ ID NO: 9. Experiments prove that the MphR protein mutant can be used for preparing erythromycin whole-cell biosensors of which the sensitivity is reduced by about 2.5 times, 10 times or even 100 times compared with the sensitivity of an original erythromycin whole-cell biosensor Top10 / pSense, and the erythromycin whole-cell biosensors can be used as high-throughput screening tools, and can be used for preparing a high-throughput screening kit. The method is applied to erythromycin detection or screening of erythromycin industrial strains with different initial yields, so that the screening flux and breeding speed of the erythromycin industrial strains are greatly improved. The method has an important application value.

The present invention provides a Bacillus subtilis strain selected from the group consisting of a) the strain deposited as DSM32324,b) the strain deposited as DSM32325, and c) a mutant strain of (a) or (b) which has sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol; and has inhibitory activity against E. coli and Clostridium perfringens. The invention further relates to Bacillus compositions comprising at least one Bacillus subtilis strain of the invention, preferably the Bacillus subtilis strain DSM32324 and / or the Bacillus subtilis strain DSM32325, as Direct Fed Microbial (DFM), premix, animal feed additive or animal feed. The invention provides a method of improving one or more animal performance parameters selected from the group consisting of i) increased weight gain (WG), ii) lower feed conversion ratio (FCR), iii) lower necrotic enteritis lesion scoring, iv) lower necrotic enteritis frequency, v) lower necrotic enteritis mortality, vi) increased European Production Efficacy Factor (EPEF), and vii) lower mortality, by feeding a strain or a composition according to the invention to an animal.

The application discloses a kind of fungus reinforced erythromycin fermentation microbial residue aerobic compost efficient detoxification and nutrient recovery method, belong to solid waste disposal and resource recycling field.The method includes that the erythromycin fermentation microbial residue is mixed after being treated with high-temperature sterilization with corn straw powder, inoculates and carries out aerobic compost fermentation with Trichoderma asperellum.The antibiotic degrading inoculum with Trichoderma asperellum as core is inoculated to reinforce the aerobic co-composting of erythromycin fermentation microbial residue and crop straw, produces nutrient content high and non-toxic harmless organic fertilizer, to provide an efficient, economical, environmentally friendly solution to solve the problem of resource utilization of erythromycin fermentation microbial residue.

The invention relates to the technical field of microbial culture, in particular to a klebsiella pneumoniae chromogenic medium as well as a preparation method and application thereof. The klebsiella pneumoniae chromogenic medium is prepared from the following raw materials: a core nutrient component, a composite salt system, a synergistic compound chromogenic system, a targeted composite antibacterial agent, a function enhancer, agar and deionized water, the synergistic compound color developing system comprises a composite color developing agent and a color developing synergist, wherein the composite color developing agent is prepared from 5-bromo-4-chloro-3-indole-beta-D-galactoside, indole acetate and naphthol-AS-D-acetate according to the weight ratio of (3.5 to 4.5) to (2.5 to 3.5) to (2.5 to 3.5); the targeted composite antibacterial agent comprises penicillin antibiotics and erythromycin in a weight ratio of (5-8): (2-4). The comprehensive advantages of high separation positive rate, comprehensive infectious microbe inhibition, high color development specificity, outstanding anti-interference capability and excellent stability are realized.

This invention relates to the field of microbial technology, specifically disclosing a strain of *Exiguobacterium indicum* capable of degrading antibiotics and its applications. The *Exiguobacterium indicum* strain is named *Exiguobacterium indicum* WC54, and was deposited at the Guangdong Provincial Microbial Culture Collection Center on October 30, 2024, with accession number GDMCC NO.65371. This strain exhibits strong removal capabilities against five different types of antibiotics—tetracyclines, sulfacetamide, doxycycline, norfloxacin, and roxithromycin—in both farmland soil and livestock waste. Compared to traditional antibiotic treatment methods, this strain has fewer limitations in terms of application environment, is more convenient to use, lower in cost, and more environmentally friendly.

The invention belongs to the technical field of insect feeding and insect resistance identification, and particularly relates to an inter-field insect medium for cotton bollworms and application. The field insect prevention medium for cotton bollworms comprises the following raw materials: soybean meal, corn flour, wheat germs, wheat bran, beer yeast powder, konjaku flour, cane sugar, Webster salt, multivitamins, ascorbic acid, linoleic acid, potassium sorbate, streptomycin sulfate, erythromycin, methyl p-hydroxybenzoate, blighted grain hull particles and water. The field insect inoculation medium for the cotton bollworms is low in raw material cost, the preparation method is simple and rapid, application is convenient, and manual insect inoculation can be completed without any professional operation. The inter-field insect killing medium provided by the invention fully meets the nutrition required by the growth and development of cotton bollworms, and can improve the inter-field insect killing effect of the cotton bollworms.

The invention relates to a ternary organic-inorganic hybrid material for adsorbing antibiotics in a water body and a preparation method, and belongs to the technical field of water treatment. The preparation method comprises the following steps: dissolving a certain amount of chitosan quaternary ammonium salt in water, and uniformly mixing to obtain a solution A; dissolving tannic acid in water, and uniformly mixing to obtain a solution B; dissolving zirconium chloride in water, and uniformly mixing to obtain a solution C; adding the solution A into the solution B, and uniformly mixing; slowly dropwise adding the solution C, quickly stirring and uniformly mixing, and reacting at room temperature; precipitating the reacted sample with ethanol and washing to obtain a gelatinous solid sample; and drying the sample in an electrothermal constant-temperature blast drying box. The material can efficiently adsorb three antibiotics in a water body, including ofloxacin, tetracycline and erythromycin; the preparation process is green and mild, short in period, low in energy consumption and good in chemical stability.

The present invention relates to a method for rapidly detecting lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water, belonging to the technical field of analytical detection. By means of specific fully automated on-line solid-phase extraction conditions in combination with specific high performance liquid chromatography-tandem mass spectrometry conditions, the present invention realizes sample enrichment, purification, separation and determination, and fully automatically analyzes lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water. The present invention realizes fully automated operation, and the total determination time only needs 11 min. The detection limit reaches the ng / L level. By washing the solid-phase extraction column with an ammonium bicarbonate aqueous solution, the problem of matrix effect commonly encountered in the analysis of complex matrix samples is overcome, meeting the requirements for ultra-trace detection of lincomycin, clindamycin, erythromycin, clarithromycin and roxithromycin in water.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The utility model provides an oculentum auxiliary material sterile treatment device which comprises an auxiliary material sterile treatment tank body, a feed port is formed in one side of the top of the auxiliary material sterile treatment tank body, and a fixing cylinder penetrates through the upper portion of the top of the auxiliary material sterile treatment tank body. Compared with the prior art, the auxiliary material sterile treatment tank has the following beneficial effects that the electromagnetic bearing in the liquid level leveling assembly and the motor output end clockwise rotation switch are started, the electromagnetic bearing drives the push plate to perform clockwise revolution in the auxiliary material sterile treatment tank body through the bending frame, and meanwhile, the threaded sleeve can drive the rotating push plate to move downwards, so that the auxiliary material sterile treatment tank body can be leveled; according to the technical scheme, the push plate can uniformly push the piled sterile erythromycin raw materials to be flat and uniformly cover the top of the light liquid paraffin below the push plate, so that the contact area between the sterile erythromycin raw materials and the light liquid paraffin is effectively enlarged, and the speed of infiltration of erythromycin by the light liquid paraffin is accelerated; therefore, the aseptic treatment processing efficiency of the oculentum auxiliary materials is effectively improved.

The invention relates to an aptamer for specifically detecting neomycin and application of the aptamer, and belongs to the technical field of detection. Firstly, a novel aptamer (the affinity reaches 1.17 + / -0.32 mu M) with good affinity to neomycin is designed, and the aptamer has no reaction to other interfering antibiotics such as chloramphenicol (CAP), oxytetracycline (OTC), enrofloxacin (ENR), azithromycin (AZT), roxithromycin (RXM) and ciprofloxacin (CIP) and has good specificity. Secondly, two fluorescence signal probes of MOF coated FITC and AgNCs are also synthesized, a ratio type fluorescence sensing system is constructed, high-precision and high-sensitivity detection of the target neomycin is realized through a double-signal ratio, and background signal influence caused by environmental interference and fluorescent dye leakage is effectively eliminated.

The invention belongs to the technical field of bacteriostatic agents, and particularly relates to pyridine ether biquaternary ammonium salt as well as a preparation method and application thereof. The method comprises the following steps: taking 3-fluoropyridine as an initial raw material, reacting with triethylene glycol to obtain 1, 2-bis (2-(pyridine-3-oxy) ethyoxyl) ethane, and carrying out quaternization reaction on the 1, 2-bis (2-(pyridine-3-oxy) ethyoxyl) ethane and brominated alkane to obtain the pyridine ether biquaternary ammonium salt. The pyridine ether biquaternary ammonium salt type bacteriostatic agent has a good bacteriostatic effect on staphylococcus aureus in gram-positive bacteria, when the concentration is 10 g / mL, the bacteriostatic rate of the bacteriostatic agent exceeds that of antibiotic erythromycin with the concentration being 40 g / mL, and the efficient and low-dosage bacteriostatic effect is achieved. The molecular structure of the pyridine ether biquaternary ammonium salt type bacteriostatic agent provided by the invention simultaneously contains three structural units with bacteriostatic activity, namely the ether bond, the pyridine ring and the quaternary ammonium salt, so that a multi-component synergistic bacteriostatic effect can be realized without compounding, and the antibacterial property is remarkably improved.

The invention provides bacillus C367 as well as a method and application thereof. The bacillus C367 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36841; the bacillus C367 is separated from the Inner Mongolia cheese; the bacillus C367 can be used for preparing medicines for treating or preventing periodontal diseases; the invention provides a novel oral cavity probiotic bacillus C367, the bacillus C367 has an inhibition effect on periodontal disease bacteria, and the bacillus C367 is sensitive to antibiotics such as minocycline, amikacin, tetracycline, erythromycin, cefoperazone, streptomycin, kanamycin, gentamicin, vancomycin and ceftriaxone, and has good survival ability in an oral cavity.

The invention provides an engineering cell for heterologous synthesis of erythromycin, a synthesis method and application. According to the invention, essential genes for heterologous synthesis of erythromycin are integrated on a prokaryotic cell chromosome; on the basis of the gene, a group of genes are knocked out in a targeted manner, so that the yield of erythromycin A is greatly improved. The invention provides a new way for breeding and industrial application of the Escherichia coli strain with high erythromycin yield.

The invention provides a method for synthesizing anthracycline compounds for treating chronic myelogenous leukemia by an enzyme method. The two anthracycline compounds are 10-decarboxyl-13-deoxyerythromycin and 10-hydroxy-13-deoxyerythromycin. The invention further provides a preparation method of the anthracycline compounds for treating the chronic myelogenous leukemia. The invention discovers and verifies that the 10-decarboxyl-13-deoxyerythromycin and the 10-hydroxy-13-deoxyerythromycin have obvious activity of resisting human chronic myeloid leukemia cells for the first time. And compared with adriamycin, the inhibition activity is improved by about 15 and 20 times. Therefore, the compound has the outstanding potential of being developed into an efficient new-generation anthracycline anti-tumor drug, and provides a new candidate drug choice for leukemia treatment.