32 results about "Erythromycin" patented technology

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The invention provides a staphylococcus aureus drug resistance prediction method based on whole genome association analysis and machine learning, and belongs to the technical field of bioinformatics and artificial intelligence crossing. Five common antibiotics such as erythromycin, ciprofloxacin, penicillin, fusidic acid and methicillin are selected as modeling objects. Performing comparison and SNP site detection on a strain genome by using a reference genome, and constructing a variation matrix; then, a pyseer tool is adopted for GWAS analysis, and genetic loci significantly related to the drug resistance of each antibiotic are screened as modeling features; and for each antibiotic, respectively constructing an independent machine learning model, inputting the SNP features screened by the GWAS, and outputting a drug resistance / sensitivity classification label. Through cross validation and independent test set evaluation, the model has excellent accuracy and F1 score on multiple antibiotics. According to the method, drug resistance prediction can be achieved based on genome data without traditional drug sensitivity experiments, and the method has the remarkable time advantage, popularization value and scientific research significance.

This invention discloses a compound ointment for protecting the nasal mucosa and repairing skin damage, and its preparation method, belonging to the field of pharmaceutical technology. The compound ointment is formulated with Sophora japonica fruit extract, erythromycin ointment, vitamin B6, levofloxacin hydrochloride tablets, loratadine tablets, fluticasone propionate nasal spray, sesame oil, and honey in specific weight proportions. The components work synergistically, providing protection for the nasal mucosa, treatment of rhinitis, reduction of cold air and external pollutant intrusion, and prevention of colds. It can also treat hemorrhoids, scars, eczema, and other skin damage, achieving "one medicine for multiple uses." This compound ointment has a stable dosage form, is convenient to use, has high utilization rate, high safety, and no obvious side effects, making it suitable for various populations and possessing good industrialization prospects.

The application provides a multi-layer dry heat sterilization device for erythromycin eye ointment production, and belongs to the technical field of dry heat sterilization cabinets. The device comprises a dry heat sterilization cabinet, a shelf is arranged in the dry heat sterilization cabinet, a tray base is arranged on the shelf, a plurality of first avoiding grooves are symmetrically arranged on the outer walls of the two sides of the tray base, first guide plates are symmetrically and fixedly connected to the inner walls of the two sides of the tray base close to the top, connecting columns are fixedly connected to the outer walls of the two sides of the tray base close to the first avoiding grooves, first handles are fixedly connected to the ends of the connecting columns away from the tray base, an auxiliary assembly is arranged on the tray base, and the auxiliary assembly is used for improving the temperature rising speed of the aluminum pipe. The auxiliary assembly can shorten the temperature rising time of the aluminum pipe of the eye ointment and reduce the operation energy consumption of the dry heat sterilization cabinet when the dry heat sterilization cabinet works.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

This invention relates to the field of industrial process automation control and waste liquid resource utilization technology, specifically to a concentration regulation system for preparing liquid fertilizer from erythromycin waste liquid based on process control. The system includes: a data acquisition terminal acquiring fluid dynamics time-series data, concentration characterization time-series data, and actuator status data; an ideal reference reconstruction module reconstructing ideal concentration data based on the fluid dynamics time-series data and actuator status data; an interference simulation module injecting interference into the ideal concentration data based on preset fouling characteristics, rheological interference characteristics, and fluid dynamics time-series data to generate simulated concentration data; a coupling decision module generating actual concentration estimation data based on the concentration characterization time-series data, and accordingly generating regulation commands including closed-loop regulation, freeze regulation, cleaning, and temperature compensation commands; and a regulation execution module responding to the regulation commands and controlling the corresponding mechanisms. This invention ensures that the actions do not excessively rely on pollution observation values.

The utility model discloses a kind of erythromycin zinc acetate ointment production stirring devices, including support frame, stirring mechanism, dustproof mechanism and heating mechanism, the stirring mechanism includes stirring barrel, first stirring device, second stirring device and connecting assembly, the stirring barrel is set on support frame upper end face, the inlet pipe is provided on the upper end surface of the stirring barrel, the first stirring device includes first motor, main shaft and first stirring blade, the first motor passes through stirring barrel and is connected with main shaft, the first stirring blade is fixedly connected with main shaft, the second stirring device includes shaft, second stirring blade, large gear and pinion, the shaft is rotatably connected with connecting assembly, the large gear and the pinion are arranged in the inside of stirring barrel, one end of the shaft is fixedly connected with pinion, the large gear is fixedly connected with the upper of the inner wall of stirring barrel, the large gear and pinion are engaged, this stirring mechanism significantly improves mixing efficiency and mixing homogeneity.

The invention discloses an MphR protein mutant and an erythromycin whole-cell biosensor constructed based on the MphR protein mutant. The amino acid sequence of the MphR protein mutant is as shown in SEQ ID NO: 5, SEQ ID NO: 7 or SEQ ID NO: 9. Experiments prove that the MphR protein mutant can be used for preparing erythromycin whole-cell biosensors of which the sensitivity is reduced by about 2.5 times, 10 times or even 100 times compared with the sensitivity of an original erythromycin whole-cell biosensor Top10 / pSense, and the erythromycin whole-cell biosensors can be used as high-throughput screening tools, and can be used for preparing a high-throughput screening kit. The method is applied to erythromycin detection or screening of erythromycin industrial strains with different initial yields, so that the screening flux and breeding speed of the erythromycin industrial strains are greatly improved. The method has an important application value.

The application discloses a kind of fungus reinforced erythromycin fermentation microbial residue aerobic compost efficient detoxification and nutrient recovery method, belong to solid waste disposal and resource recycling field.The method includes that the erythromycin fermentation microbial residue is mixed after being treated with high-temperature sterilization with corn straw powder, inoculates and carries out aerobic compost fermentation with Trichoderma asperellum.The antibiotic degrading inoculum with Trichoderma asperellum as core is inoculated to reinforce the aerobic co-composting of erythromycin fermentation microbial residue and crop straw, produces nutrient content high and non-toxic harmless organic fertilizer, to provide an efficient, economical, environmentally friendly solution to solve the problem of resource utilization of erythromycin fermentation microbial residue.

The invention relates to the technical field of microbial culture, in particular to a klebsiella pneumoniae chromogenic medium as well as a preparation method and application thereof. The klebsiella pneumoniae chromogenic medium is prepared from the following raw materials: a core nutrient component, a composite salt system, a synergistic compound chromogenic system, a targeted composite antibacterial agent, a function enhancer, agar and deionized water, the synergistic compound color developing system comprises a composite color developing agent and a color developing synergist, wherein the composite color developing agent is prepared from 5-bromo-4-chloro-3-indole-beta-D-galactoside, indole acetate and naphthol-AS-D-acetate according to the weight ratio of (3.5 to 4.5) to (2.5 to 3.5) to (2.5 to 3.5); the targeted composite antibacterial agent comprises penicillin antibiotics and erythromycin in a weight ratio of (5-8): (2-4). The comprehensive advantages of high separation positive rate, comprehensive infectious microbe inhibition, high color development specificity, outstanding anti-interference capability and excellent stability are realized.

This invention relates to the field of microbial technology, specifically disclosing a strain of *Exiguobacterium indicum* capable of degrading antibiotics and its applications. The *Exiguobacterium indicum* strain is named *Exiguobacterium indicum* WC54, and was deposited at the Guangdong Provincial Microbial Culture Collection Center on October 30, 2024, with accession number GDMCC NO.65371. This strain exhibits strong removal capabilities against five different types of antibiotics—tetracyclines, sulfacetamide, doxycycline, norfloxacin, and roxithromycin—in both farmland soil and livestock waste. Compared to traditional antibiotic treatment methods, this strain has fewer limitations in terms of application environment, is more convenient to use, lower in cost, and more environmentally friendly.

The invention belongs to the technical field of insect feeding and insect resistance identification, and particularly relates to an inter-field insect medium for cotton bollworms and application. The field insect prevention medium for cotton bollworms comprises the following raw materials: soybean meal, corn flour, wheat germs, wheat bran, beer yeast powder, konjaku flour, cane sugar, Webster salt, multivitamins, ascorbic acid, linoleic acid, potassium sorbate, streptomycin sulfate, erythromycin, methyl p-hydroxybenzoate, blighted grain hull particles and water. The field insect inoculation medium for the cotton bollworms is low in raw material cost, the preparation method is simple and rapid, application is convenient, and manual insect inoculation can be completed without any professional operation. The inter-field insect killing medium provided by the invention fully meets the nutrition required by the growth and development of cotton bollworms, and can improve the inter-field insect killing effect of the cotton bollworms.

The invention relates to a ternary organic-inorganic hybrid material for adsorbing antibiotics in a water body and a preparation method, and belongs to the technical field of water treatment. The preparation method comprises the following steps: dissolving a certain amount of chitosan quaternary ammonium salt in water, and uniformly mixing to obtain a solution A; dissolving tannic acid in water, and uniformly mixing to obtain a solution B; dissolving zirconium chloride in water, and uniformly mixing to obtain a solution C; adding the solution A into the solution B, and uniformly mixing; slowly dropwise adding the solution C, quickly stirring and uniformly mixing, and reacting at room temperature; precipitating the reacted sample with ethanol and washing to obtain a gelatinous solid sample; and drying the sample in an electrothermal constant-temperature blast drying box. The material can efficiently adsorb three antibiotics in a water body, including ofloxacin, tetracycline and erythromycin; the preparation process is green and mild, short in period, low in energy consumption and good in chemical stability.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The invention relates to an aptamer for specifically detecting neomycin and application of the aptamer, and belongs to the technical field of detection. Firstly, a novel aptamer (the affinity reaches 1.17 + / -0.32 mu M) with good affinity to neomycin is designed, and the aptamer has no reaction to other interfering antibiotics such as chloramphenicol (CAP), oxytetracycline (OTC), enrofloxacin (ENR), azithromycin (AZT), roxithromycin (RXM) and ciprofloxacin (CIP) and has good specificity. Secondly, two fluorescence signal probes of MOF coated FITC and AgNCs are also synthesized, a ratio type fluorescence sensing system is constructed, high-precision and high-sensitivity detection of the target neomycin is realized through a double-signal ratio, and background signal influence caused by environmental interference and fluorescent dye leakage is effectively eliminated.

The invention belongs to the technical field of bacteriostatic agents, and particularly relates to pyridine ether biquaternary ammonium salt as well as a preparation method and application thereof. The method comprises the following steps: taking 3-fluoropyridine as an initial raw material, reacting with triethylene glycol to obtain 1, 2-bis (2-(pyridine-3-oxy) ethyoxyl) ethane, and carrying out quaternization reaction on the 1, 2-bis (2-(pyridine-3-oxy) ethyoxyl) ethane and brominated alkane to obtain the pyridine ether biquaternary ammonium salt. The pyridine ether biquaternary ammonium salt type bacteriostatic agent has a good bacteriostatic effect on staphylococcus aureus in gram-positive bacteria, when the concentration is 10 g / mL, the bacteriostatic rate of the bacteriostatic agent exceeds that of antibiotic erythromycin with the concentration being 40 g / mL, and the efficient and low-dosage bacteriostatic effect is achieved. The molecular structure of the pyridine ether biquaternary ammonium salt type bacteriostatic agent provided by the invention simultaneously contains three structural units with bacteriostatic activity, namely the ether bond, the pyridine ring and the quaternary ammonium salt, so that a multi-component synergistic bacteriostatic effect can be realized without compounding, and the antibacterial property is remarkably improved.

The invention provides bacillus C367 as well as a method and application thereof. The bacillus C367 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36841; the bacillus C367 is separated from the Inner Mongolia cheese; the bacillus C367 can be used for preparing medicines for treating or preventing periodontal diseases; the invention provides a novel oral cavity probiotic bacillus C367, the bacillus C367 has an inhibition effect on periodontal disease bacteria, and the bacillus C367 is sensitive to antibiotics such as minocycline, amikacin, tetracycline, erythromycin, cefoperazone, streptomycin, kanamycin, gentamicin, vancomycin and ceftriaxone, and has good survival ability in an oral cavity.

The invention provides a method for synthesizing anthracycline compounds for treating chronic myelogenous leukemia by an enzyme method. The two anthracycline compounds are 10-decarboxyl-13-deoxyerythromycin and 10-hydroxy-13-deoxyerythromycin. The invention further provides a preparation method of the anthracycline compounds for treating the chronic myelogenous leukemia. The invention discovers and verifies that the 10-decarboxyl-13-deoxyerythromycin and the 10-hydroxy-13-deoxyerythromycin have obvious activity of resisting human chronic myeloid leukemia cells for the first time. And compared with adriamycin, the inhibition activity is improved by about 15 and 20 times. Therefore, the compound has the outstanding potential of being developed into an efficient new-generation anthracycline anti-tumor drug, and provides a new candidate drug choice for leukemia treatment.

The application relates to a preparation method of an animal model of pediatric anorexia and application thereof, and belongs to the technical field of medicines. The model can be used for screening of medicines for treating children anorexia, pharmacological research and efficacy evaluation. The application first creatively adopts a modeling method of gradient increment gavage of erythromycin to successfully establish an animal model of children anorexia. The modeling method has the following advantages compared with a conventional high-fat high-protein fat feed feeding and central operation modeling method: modeling time is shortened; compared with other modeling methods, the modeling time cost is reduced by two weeks; economic cost is reduced; compared with high-fat high-protein feed which is relatively expensive, the cost of erythromycin medicine is 1 / 10 of that of the high-fat high-protein feed, and the reduction of time cost also reduces the cost of animal feeding; the operation technology is simple and convenient; compared with the preparation of a high-precision central anorexia animal model, the experimental operation technology is simple; the requirement for laboratory instruments and equipment is relatively low; the demand for experimental personnel is reduced, and the labor cost is reduced.

The invention belongs to the field of insect feeding and insect resistance identification, and particularly relates to an inter-field insect medium for armyworms and / or grassland spodoptera exigua and application. The invention provides an inter-field insect medium for armyworms and / or grassland spodoptera exigua, which comprises puffed corn flour, puffed soybean flour, corn leaf meal, wheat germ, beer yeast, milk powder, wheat bran, casein, ascorbic acid, multivitamin glucose, erythromycin, sodium sorbate, methyl p-hydroxybenzoate, propyl p-hydroxybenzoate, cholesterol, inositol and streptomycin sulfate. Vermiculite and water. The raw materials of the field insect inoculation medium are easy to obtain, the cost is low, preparation is easy, storage is convenient, the field large-scale artificial insect inoculation efficiency of armyworms and spodoptera frugiperda can be improved, the influence of natural factors on the insect inoculation effect is reduced, meanwhile, the stability and repeatability of the insect inoculation effect are guaranteed, and optimization of the economic cost is achieved.

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.

The invention relates to the technical field of biological medicine, and particularly discloses erythromycin nano-liposome as well as a preparation method and application thereof. Vitamin E acetate and resveratrol are adopted to construct a dual stable system, a high-pressure homogenization method is adopted to prepare the erythromycin nano-liposome, and the erythromycin nano-liposome has the advantages of high transdermal efficiency, excellent bioavailability, strong stability, high encapsulation efficiency, accurate targeting, medication safety and the like. On the basis, the invention further provides the erythromycin in-situ gel which is good in clinical efficacy, safe and comfortable in medication and good in stability and biocompatibility, meanwhile, the appearance smoothness after wound healing can be improved, and the dual requirements of patients for wound healing efficiency and healing quality can be better met. The invention can be applied to the field of external preparations.

This utility model discloses an emergency kit for children's nosebleeds, comprising: a nasal hemostatic agent, cotton swabs, erythromycin ointment, and a packaging box. The nasal hemostatic agent includes a reservoir stick, a sponge strip, a push rod, and a sealing strip. The reservoir stick includes a base plate, side walls, and a top sealing film. The sponge strip is disposed outside the reservoir stick, and its bottom is connected to the top sealing film. The push rod passes through the base plate, with a mesh support connected to one end of the push rod, which is disposed inside the reservoir stick. The other end of the push rod has a push handle disposed outside the reservoir stick. A sealing strip is provided on the base plate to prevent leakage between the base plate and the push rod. This utility model provides an emergency kit for children's nosebleeds that can simultaneously achieve hemostasis through medication and physical pressure, effectively addressing nosebleeds in children and preventing the residue of fibrous material. The operation is simple, and the kit is easy to use. It can reduce the rate of crust formation, inhibit inflammatory reactions, and help maintain good moisture in the nasal mucosa.

The utility model discloses a kind of ointment production vibration feeding mechanisms, it is related to medical relevant technical field.The utility model includes storage hopper, cloth shell, transfer shell, feeding inclined pipe and vibration motor, the outside of storage hopper is provided with cloth shell, the bottom of storage hopper is fixed with guide chute, the cloth shell side of each through slot is fixed with connecting shell, the bottom of cloth shell is fixed with transfer shell, and vibration motor is fixed on the outside of the one side of transfer shell far from storage hopper;The bottom of transfer shell is fixed with equidistantly distributed feeding inclined pipe, and the bottom of each feeding inclined pipe is fixed with feeding arc pipe.The utility model is through being arranged storage hopper, transfer shell, cloth shell, vibration motor, feeding inclined pipe, feeding arc pipe, connecting shell, guide chute, solve the problem that the bridging phenomenon is easily generated when erythromycin is continuously fed in small amount and stops feeding, and the feeding height of erythromycin cannot be changed.

The invention discloses a synthetic method of a clarithromycin preparation promoted by ionic liquid and the clarithromycin preparation. The synthetic method comprises an etherification reaction, a silanization reaction and a methylation reaction. The etherification reaction comprises the following steps: adding erythromycin A-9-oxime into a reaction container, and adding ionic liquid [Bmim] BF4 as a reaction solvent. The silanization reaction comprises the following steps: adding 1, 1, 1, 3, 3, 3-hexamethyl disilazane and imidazole into a reaction container containing an etherification reaction result feed liquid of the [Bmim] BF4 reaction solvent. The methylation reaction comprises the step of adding methyl bromide and potassium hydroxide into a reaction container containing silanization reaction result feed liquid of the [Bmim] BF4 reaction solvent. The three-step reaction of etherification, silanization and methylation is carried out in the same reactor, and products in all steps do not need to be separated, so that a continuous process is realized. The ionic liquid as a reaction solvent is non-volatile and non-flammable, and is an important path for realizing green synthesis and clean production.

The application discloses application of bicyclic phloroglucinols in preparation of antibacterial drugs, and relates to the technical field of medicines.Bicyclic phloroglucinols (BPs) synthesized based on the skeleton of natural phloroglucinols have universal inhibitory activity on gram-positive bacteria, and the BPs can effectively reduce the effective concentration of common antibiotics (vancomycin, norfloxacin, meropenem, erythromycin and rifampicin) on bacteria, enhance the antibacterial effect of the antibiotics, and expand the antibacterial spectrum of the antibiotics. S. aureus The BPs involved in the application have the advantages of brand-new structure, high antibacterial activity, synergistic effect when combined with antibiotics, and the like, have great potential to be prepared into antibacterial drugs, and have high commercial potential.

The invention relates to the technical field of pharmaceutical preparation detection, in particular to a method for rapidly detecting microbial limit of an erythromycin ointment preparation, which comprises the following steps: S1, pretreating and demulsifying a sample; s2, performing enzymolysis and ultrasonic unwrapping; s3, amphiphilic neutralization: adding a polyoxyethylated castor oil-cysteine copolymer neutralizer into the system treated in the step S2, incubating, and removing oil phase residues; step S4, nano fluorescence sensitization; step S5, filtering and enriching; and step S6, bioluminescence detection: adding a luminescent reagent into the microorganisms enriched in the step S5 for incubation, detecting a relative luminescence unit, and calculating the concentration of the sample microorganisms in combination with the standard curve. By constructing a six-step innovative process of pretreatment, enzymolysis, neutralization, sensibilization, enrichment and detection, key parameters such as the concentration of the composite demulsifier and enzymolysis time are optimized, the detection limit reaches 10 CFU / mL, the detection time is smaller than or equal to 1 h, and the microbiological detection accuracy and efficiency of the erythromycin ointment are remarkably improved.

The invention belongs to the technical field of microorganisms, and particularly relates to a Bifidobacterium longum subsp. Longum FMBL B250365 MQ which is preserved in the China Center for Type Culture Collection on September 18, 2025, has the preservation number of CCTCC M 20252060, can utilize oligosaccharides such as fructo-oligosaccharide, galactooligosaccharide, maltooligosaccharide, soybean oligosaccharide and the like, and can be used for preparing a feed additive, a feed additive and a feed additive. Good tolerance to acid and cholate is achieved; the compound is sensitive to ampicillin, streptomycin, tetracycline, chloramphenicol, vancomycin, erythromycin, clindamycin and gentamicin; the drug resistance to kanamycin is realized; the caenorhabditis elegans has antioxidant and bacteriostatic capabilities, and the average life of caenorhabditis elegans is prolonged by 18.65%; the compound can be used for preparing anti-aging and life-prolonging medicines, and has a wide application prospect.

The invention relates to a hybridoma cell strain capable of secreting a tildipirosin monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain provided by the invention has good sensitivity and specificity to the tildipirosin, wherein the IC50 value to the tildipirosin is 20 ng / mL. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of tildipirosin, such as erythromycin, streptomycin, azithromycin and spiramycin, and has good specificity, so that low-concentration tildipirosin can be accurately detected.