463 results about "Erythromycin" patented technology

Several diterpene glycosides (e.g., rubusoside, rebaudioside, steviol monoside and stevioside) were discovered to enhance the solubility of a number of pharmaceutically and medicinally important compounds, including but not limited to, paclitaxel, camptothecin, curcumin, tanshinone HA, capsaicin, cyclosporine, erythromycin, nystatin, itraconazole, and celecoxib. The use of the diterpene glycoside rubusoside increased solubility in all tested compounds. The diterpene glycosides are a naturally occurring class of water solubility-enhancing compounds that are non-toxic and that will be useful as new complexing agents or excipients in the pharmaceutical, agricultural (e.g., solubilizing pesticides), cosmetic and food industries. Aqueous solutions by using rubusoside to increase the solubility of otherwise insoluble drugs will have several new routes of administration. In addition, aqueous solutions of therapeutic compounds with rubusoside were shown to retain the known pharmacological activity of the compounds.

A series of novel steroid derivatives are described. The steroid derivatives are antibacterial agents. The steroid derivatives also act to sensitize bacteria to other antibiotics including erythromycin and novobiocin.

Disclosed is a pharmaceutical composition for extended release of an erythromycin derivative in the gastrointestinal environment. The composition comprises an erythromycin derivative and a pharmaceutically acceptable polymer so that, when ingested orally, the composition induces statistically significantly lower Cmax in the plasma than an immediate release composition of the erythromycin derivative while maintaining bioavailability and minimum concentration substantially equivalent to that of the immediate release composition of the erythromycin derivative upon multiple dosing. The compositions of the invention have an improved taste profile and reduced gastrointestinal side effects as compared to those for the immediate release composition.

A series of novel steroid derivatives are described. The steroid derivatives are antibacterial agents. The steroid derivatives also act to sensitize bacteria to other antibiotics including erythromycin and novobiocin.

The present invention features rifamycin analogs that can be used as therapeutics for treating or preventing a variety of microbial infections. In one form, the analogs are acetylated at the 25-position, as is rifamycin. In another form, the analogs are deacetylated at the 25-position. In yet other forms, benzoxazinorifamycin, benzthiazinorifamycin, and benzdiazinorifamycin analogs are derivatized at various positions of the benzene ring, including 3′-hydroxy analogs, 4′-and / or 6′ halo and / or alkoxy analogs, and various 5′ substituents that incorporate a cyclic amine moiety.

A method of producing an N-demethyl-N-isopropyl-8,9-anhydroerythromycin A-6,9-hemiacetal or a salt thereof, characterized in that an N-demethylerythromycin A or a salt thereof is reacted with an isopropylating agent and subsequently treated under acidic conditions, and a method of producing a substantially pure crystal of an 8,9-anhydroerythromycin A-6,9-hemiacetal derivative represented formula (VI): wherein R1 and R2, whether identical or not, represent an alkyl having 1 to 6 carbon atoms, an alkenyl having 2 to 6 carbon atoms or an alkynyl having 2 to 6 carbon atoms; R3 represents hydrogen or a hydroxyl group; one of R4 and R5 represents hydrogen and the other represents a hydroxyl group, or R4 and R5 bind together to represent O=; R6 represents hydrogen or a hydroxyl group that may be substituted for; R7 represents hydrogen or a hydroxyl group; or a salt thereof, characterized in that a crude crystal of said 8,9-anhydroerythromycin A-6,9-hemiacetal derivative or a salt thereof is recrystallized as a solvation product from hydrated isopropanol.

The invention discloses a method for preparing azithromycin. The method comprises the following steps of: in acid aqueous solution and under an action of potassium borohydride, performing the reduction reaction of erythromycinA6, 9-imine ether represented by a formula II, then performing methylation reaction, and putting the reaction products in a mixed solvent of water and halogenated hydrocarbons for hydrolysis reaction so as to obtain the azithromycin represented by a formula I. In the method, the reducer is properly consumed, the yield is high, and the cost of the preparation of the azithromycin is obviously reduced. The invention also relates to a method for preparing the erythromycinA6, 9-imine ether and erythromycinA9-oxime.

The present invention features rifamycin analogs that can be used as therapeutics for treating or preventing a variety of microbial infections. In one form, the analogs are acetylated at the 25-position, as is rifamycin. In another form, the analogs are deacetylated at the 25-position. In yet other forms, benzoxazinorifamycin, benzthiazinorifamycin, and benzdiazinorifamycin analogs are derivatized at various positions of the benzene ring, including 3′-hydroxy analogs, and / or various fused ring systems with the benzene ring at the 4′,5′ or 5′,6′ positions.

A process for the use of low concentration levels of Erythromycin to eliminate or control the growth of unwanted or undesirable bacteria (contaminating bacteria) in the fermentation production of alcohols without inhibition of the growth or replication of the yeast.

The invention discloses water, ethanol and ethanol aqueous extracts of traditional Chinese medicine pithecellobium clypearia and application of corresponding petroleum ether, ethyl acetate, normal butanol and a water extractants in preparation of medicines for treating methicillin-resistant staphylococcus aureus (MRSA) and antibiotics anti-MRSA sensitization medicines. Meanwhile, the invention further discloses a preparation method of the extracts or extractants. Experimental results show that water, 10% ethanol, 30% ethanol, 60% ethanol, 95% ethanol extracts of pithecellobium clypearia and corresponding ethyl acetate, normal butanol and water extractants have stronger anti-MRSA effect, wherein the activity of the ethyl acetate extracting part of the 60% ethanol extract of pithecellobium clypearia is the strongest, and the ethyl acetate extracting part of the 60% ethanol extract of pithecellobium clypearia has sensitization effect on erythrocin, ceftriaxone sodium, levofloxacin for treating MRSA.

Compounds of formula (I) wherein A is and R1, R2, R3, and R4 are as defined herein, are prokinetic agents and can be used to treat disorders of gastric motility.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

A micropill-type enteric tablet of erythromycin is proportionally prepared from erythromycin, filler, adhesive, lubricant, flowing aid, isolating coating and enteric coating.

The invention discloses an engineering bacterium through anthracene nucleus antibiotic and appliance, which is characterized by the following: inserting or replacing restriction enzymes site of dnmV gene nucleic acid order of sky blue rufus streptomycete with antibiotic against property gene and surface cerubidin synthetic gene; blocking-up engineering bacterium of dnmV gene; or transforming expressing carrier of anthracene nucleus antibiotic synthetic gene to lead streptomycin (S.lividans)TK24; extracting expressing plasmid from S.lividans TK24; leading-in sky blue rufus streptomycete gene only with antibiotic against property gene to block dnmV gene; blocking-up abrupt change bacterium. This invention can produce cerubidin and / or analogue, such as anthracene nucleus antibiotic.

The invention discloses a method for preparing a magnetic molecularly-imprinted adsorbent and belongs to the technical field of preparation of environment-friendly functional materials. The method comprises the following steps of dispersing nanometer chitosan particles serving as stable particles in a solution as a water phase; mixing toluene serving as an oil-phase solvent and a pore-forming agent, methyl methacrylate serving as a functional monomer, ethylene glycol di(methacrylate) serving as a crosslinking agent, 2,2'-azobisisobutyronitrile serving as an initiator and magnetic carrier oil acid-modified nanometer ferriferrous oxide and molecularly-imprinted erythromycin to obtain a mixture serving as an oil phase; mixing the oil phase and the water phase to form a stable Pickering emulsion, preparing magnetically imprinted polymer microspheres by virtue of heat-induced reaction and applying the magnetically imprinted polymer microspheres in the adsorption and separation of erythromycin in an aqueous solution. The product has the imprinted hole and magnetic characteristics and is conducive to specific adsorption of target molecules, the magnetic carrier is conducive to the separation of the adsorbent and internal imprints can prevent the automatic falling and magnetic flux leakage of the target molecules after the target molecules are adsorbed and a better absorption effect is achieved.

The invention discloses a fermentation method of erythromycin fermentation liquid, comprising the following steps of: inoculating streptomyces erythreus (producing bacterium) spores, culturing in a slant culture medium, performing three-stage seed intermediate culture, then inoculating into a sterilized culture medium, introducing sterile air and stirring, adding nutrient substances, and culturing. According to the invention, by optimizing the culture medium and improving the fermentation method, the utilization ratios of raw material such as glucose, soybean meal, corn steep liquor and the like are improved, the kinds and consumption of raw and auxiliary materials for fermentation are reduced, and the bioavailability of the raw and auxiliary materials is high.

The invention relates to a method for producing an organic fertilizer by using erythromycin waste residue and tetracycline urea double salt mother liquor. The method is characterized by comprising the following steps: evenly mixing erythromycin waste residue hydrolysate obtained by heating and hydrolyzing under an acid condition with dialysate of the tetracycline urea double salt mother liquor separated by an organic membrane in a manner of nanofiltration separation at a volume ratio of (2-3):1; then adding 5-10% of ammonium humate and 10-15% of bentonite based on total volume; evenly stirring, detecting that the total nutrient (N+P2O5+K2O) is greater than or equal to 2.0%, the organic matter is greater than or equal to 20% and the dry matter is greater than or equal to 35%; spraying and pelletilizing when the PH is 5.5-8.5, drying until the moisture is smaller than or equal to 20%, and sieving, so as to obtain the product, namely the organic fertilizer. By adopting the method, the organic fertilizer is prepared from the hydrolyzed erythromycin waste residue hydrolysate obtained by hydrolytic degradation and the dialysate of the tetracycline urea double salt mother liquor separated by the organic membrane by nanofiltration separation in a mixing manner, so that the problem of environmental pollution of antibiotic residues is ended; reutilization of resources is taken in; new economic benefits are brought about for enterprises; furthermore, the method is sufficient in raw materials, and low in manufacturing cost.

The invention discloses a preparation method of azithromycin intermediate, in particular to a preparation method of azithromycin intermediate 9- deoxidation-alpha- heterocyclic nitrogen-9a-erythromycin A, belonging to the field of pharmaceutical chemical synthesis. The preparation method comprises the steps of: in acid aqueous solution, under the action of potassium borohydride or sodium borohydride, carrying out reduction reaction on erythromycin A 6, 9-imine ether, adding organic saccharic acid for hydrolysis reaction, and obtaining the 9-deoxidation-alpha-heterocyclic nitrogen-9a-erythromycin A. By adopting the method, the hydrolysis of boric acid ester is thorough, the products of acid degradation are less, the yield is high, the production cost of azithromycin is remarkably reduced, and the industrialized production can be very smoothly carried out.

The invention discloses gene recombination mediated on the basis of phiC312 and inheritance reformation for an erythrocin producing bacterium. A target gene can be stably integrated on a locus pre-constructed by a host cell genome by a method of gene recombination mediated by a phiC312 locus specificity integration mechanism. The invention also relates to an application of the method on the inheritance reformation of the erythrocin producing bacterium.

The invention provides an erythromycin enteric-coated capsule and the preparation method. The invention is characterized in that: the component ratio of the enteric-coated liquid of the enteric-pellets capsules contained in the enteric-coated capsules counted by weight percentage is that: polyacrylic resin: II: 1, diethyl phthalate: 0.07 to 0.1, Tween-80:0.08 to 0.12, castor oil: 0.08 to 0.12, ethanol whose concentration is bigger than or equal to 95%: 12 to 14; all are counted as weight percentage. The invention conquers defects in the prior art, elevates the product quality, keeps the releasing degree of the product stable and guarantees the quality as well as healing effect of the product during the period of validity, and also the preparation is simple and convenient, the work efficiency is high and the quality of the products is quite well.

The invention discloses a formula of an artificial feed for dichocrocis punctiferalis, and a manufacturing method of the artificial feed and belongs to the field of artificial feeds for insects. The feed comprises Chinese chestnut powder, maize meal, peach powder, soybean flour, beer yeast powder, multivita-glucose, cholesterol, casein, ascorbic acid, erythromycin, agar powder, methylparaben, methyl parahydroxybenzoats and water. The invention further provides a manufacturing method of the artificial feed for dichocrocis punctiferalis. According to the artificial feed, the Chinese chestnut powder, the maize meal, the soybean flour, the beer yeast powder and the like are added and are in coordination with a plurality of trace elements, so that on the premise of improving the survival rate, the fecundity and the like of dichocrocis punctiferalis, the raising cost is lowered effectively, and the problem that when the conventional artificial feed is used for feeding dichocrocis punctiferalis, larvae refuse feed, the survival rate is low, and the development is not uniform can be solved.

The invention relates to method for purifying erythromycin A from fermented liquid by adopting a resin adsorption method, which includes the following steps: (1) adsorption, (2) washing of impurities, (3) desorption, (4) salifying of eluent and the like. The method overcomes the defects of lower elution rate of 1BV erythromycin, more impurity contents in products and the like in the existing resin adsorption and separation technology. In addition, the invention also provides a resin regeneration method which includes the following main steps: mixed solution consisting of 0.1 mol / L-1.0 mol / L NaOH aqueous solution and a water soluble or partially water soluble organic solvent is adopted for washing resin to be regenerated, so as to solve the problem of poor resin regeneration effect in the prior art.

The invention discloses a method for measuring residual erythromycin in an environment by using a hydrophilic ionic-liquid aqueous two-phase system. The method comprises the following steps of: forming the ionic-liquid aqueous two-phase system by using 1.5 grams of 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4) and 11 grams of NaH2PO4, at least extracting the solution for 2 hours once by using the extracting system, standing the solution after extraction, measuring the concentration of the residual erythromycin in the water phase after the split phases are clear, and calculating the extraction rate of the erythromycin by using the erythromycin content of the water phase before and after the extraction. According to the method, the extraction rate of the erythromycin can reach 99.31 percent. The method has the characteristics of wide linear range, low detection limit, small relative standard deviation and high measuring yield of a sample. The method can meet the national detection requirement for the residual erythromycin, is simpler to operate, and is suitable for quantitative analysis of residual trace erythromycin in food.

The invention relates to a high efficiency liquid phase chromatogram method for detecting erythromycin in cosmetics, which is characterized in that: a C8 column or a C18 column with octadecylsilane chemically bonded silica as fillers is adopted; an ultraviolet detector or a diode array detector is used to conduct detection at the position of 190-380nm, ammonium dihydrogen phosphate solution-acetonitrile buffer solution or ammonium dihydrogen phosphate solution-methanol buffer solution is used as mobile phase. The sample is extracted by dried methyl alcohol or acetonitrile, and then extracted by ultrasonic wave for 20 min, and then centrifuged for 5 min at the speed of 12000rpm / min; supernatant solution is taken out as the liquid for test, and can be filtered for later use by means of 0.45 Mu m filter membrane if necessary; qualitative analysis is conducted according the keeping time of the sample and the spectrogram, and quantitative analysis can be performed according to the peak area. The invention has the advantages of high definition and sensitivity, good reproducibility and selectivity, and simple and quick pre-processing method of sample; therefore; the detection method is applicable to the microanalysis of erythromycin in cosmetics.

The invention relates to a derivative of tetra cyclic anthroquinones antibiotics with anti-cancer activity, namely, the compound shown by formula (I). The derivative of the tetra cyclic anthroquinones antibiotics has the activity equal to or even higher than that of the known medicines such as doxorubicin, daunorubicin and the like at cell level and simultaneously has better tolerance than the doxorubicin on animal bodies.

The invention discloses an erythromycin A derivative and a preparation method thereof. R1 and R2 are independently H, C1-C6 alkyl groups, substituted or unsubstituted C6-C10 aromatic groups, or substituted or unsubstituted C4-C8 heteroaryls; or R1 and R2 are connected to form (CH2)n, wherein n is 2-7; substitutional groups of the aromatic groups or the heteroaryls are one or more of C1-C6 alkoxyl groups, the C1-C6 alkyl groups and the C6-C10 aromatic groups; a hetero atom in the heteroaryls is S, O or N; R is H or groups shown in the description; R3 is the C1-C6 alkyl groups substituted by the substituted or unsubstituted C6-C10 aromatic groups; the substitutional groups of the C6-C10 aromatic groups are nitro groups; and R4 is carbonyl groups or H substituted by the C1-C6 alkyl groups. The compound disclosed by the invention has stronger antibiotic activity, particular for gram positive bacteria.

The invention provides an animal nutrition pelletized feedstuff and preparation method thereof, wherein the feed stuff is prepared from rice straw stem, maize stalk, banana stems, red radish, soya bean waste, pachyrhizus, cassava, sugar-cane, rank grass, beef-wood, oxytetracycline, honeysuckle flower, wild chrysanthemum flower, erythrocin, lycine, banthionine, threonine, yeasts, sea fish meal, and fresh water fish.

The invention discloses a streptomycete constitutive promoter and applications thereof, and provides an DNA fragment. The DNA fragment is any one selected from the following DNA molecules in 1)-3): 1) an DNA molecule represented by the sequence 1 in a sequence table; 2) an DNA molecule with promoter functions and hybrid with the DNA molecule shown in 1) under strict conditions; 3) an DNA molecule with more than 90% of homology with the DNA molecule shown in 1) and with promoter functions. The experiments shows that a strong constitutive promoter kasO*p is screened out, and the promoter and the erythromycin promoter ermE*p are compared in representation of transcriptional level and expression level in streptomyces coelicolor, streptomyces avermitilis and streptomyces venezuelae, and the results show that the modified promoter kasO*p is better than the the erythromycin promoter ermE*p.

The invention relates to a culturing medium and fermentation method for producing erythrocin through fermentation of streptomyces erythreus. A primary seed medium, a secondary seed medium and a fermentation medium for producing erythrocin through fermentation of streptomyces erythreus all contains sucrose and a corn steep liquor. According to the invention, the corn steep liquor, urea, sucrose, and other intermediate-acting carbon sources and nitrogen sources are adopted to substitute original slow-acting carbon sources and nitrogen sources; moreover, the corn steep liquor, urea, and sucrose are soluble in water, so that the viscosity of the fermentation liquor is lowered, the dissolved oxygen is increased, the utilization speed is increased, especially during the later period of fermentation, the solid residue at the end of the fermentation is reduced, the bacteria dregs and the BOD content and the COD contend in the waste water are all greatly reduced, energy-reserving and consumption-lowering are both realized, and the good effects of treatment from the source and clean production are achieved.

The invention relates to the cloning, sequencing, analyzing, functional study of biological synthetic gene cluster of erythromycin produced by streptomycete in tetrahydroisoquinoline alkaloid family which has the antineoplastic activity and application thereof. The whole gene cluster contains 30 genes: 3 nonribosome polypeptide synthetase genes, 4 antecedent molecule synthetic genes of 3-hydroxy-5-methyl-O-methyl-tyrosine, 2 peptid ring skeleton synthetic genes, 7 erythromycin modifying genes, 5 genes related to S-adenosine methionine synthesis, 3 regulation genes, 2 resistance genes and 4 genes with uncertain function. Genetic manipulation of the genes can block the synthesis of erythromycin, change the output and get analogue of erythromycin. The gene cluster can be used for gene engineering, protein expression, enzymic catalytic reaction, etc. of tetrahydroisoquinoline alkaloid family and finding out compounds or genes being suitable for medicine, industry or agriculture.