1096 results about "Acetylation" patented technology

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant staphylococcus aureus and application of the antibacterial peptide. The antibacterial peptide is obtained by performing N-terminal acetylation modification and C-terminal amidation modification on polypeptide as shown in SEQ ID NO.1. The invention further discloses a preparation method of the antibacterial peptide. The antibacterial peptide provided by the invention is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide to pathogenic bacteria staphylococcus aureus ATCC12600 and staphylococcus aureus ATCC33591 are respectively 4 [mu] g / mL and 32 [mu] g / mL, the biological safety is extremely high, and due to the characteristics, the antibacterial peptide shows extremely high medical drug antibacterial value, and can be used for preparing the antibacterial peptide. Alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The invention discloses a degradable bamboo fiber express packaging bag and a preparation method thereof, and belongs to the technical field of express packaging. The packaging bag is prepared from, by mass, 32-42 parts of pretreated bamboo fibers, 22-28 parts of poly (butylene succinate), 9-14 parts of modified nano calcium carbonate, 12-16 parts of polycaprolactone, 6-11 parts of acetylated chitosan and the like, and the key raw materials are all treated through an exclusive pretreatment or modification process. The preparation method comprises the steps of raw material plasma activation, segmented high-speed mixing, vacuum drying, precise-parameter twin-screw extrusion granulation, secondary drying, precise film blowing, high-precision heat-sealing bag making and the like, and the technological parameters of all the steps are clear. The product disclosed by the invention can be efficiently degraded in a natural environment, has excellent tensile resistance, puncture resistance and impact resistance, is stable and controllable in processing process, and is adaptive to actual requirements of express packaging and industrial batch production.

The invention provides a method for regulating and controlling the alcoholysis degree of polyvinyl alcohol, which comprises the following steps: carrying out acetylation reaction on a polyvinyl alcohol raw material and an acetylation reagent to obtain a polyvinyl alcohol product with the alcoholysis degree different from that of the polyvinyl alcohol raw material. According to the method for regulating and controlling the alcoholysis degree of polyvinyl alcohol provided by the invention, polyvinyl alcohol is taken as a raw material, acetyl is reversely inoculated through acetylation reaction, polyvinyl alcohol products with different alcoholysis degrees can be obtained, and the method is relatively high in stability, simple and suitable for large-scale production.

The present application discloses an acetylated glucomannan electrospun membrane, and a preparation method therefor and a use thereof. The preparation method for an electrospun membrane of the present application comprises: first adding glucomannan into a mixed solution of pyridine and acid anhydride, carrying out solid-liquid separation after the reaction, collecting a solid phase, adding ethanol for precipitation, and collecting the precipitate to obtain acetylated glucomannan; then dissolving the acetylated glucomannan in a mixed solution of chloroform and N,N-dimethylformamide, and carrying out ultrasonic treatment to obtain an electrospinning solution; and finally, using the electrospinning solution as a raw material, and carrying out electrospinning to obtain an acetylated glucomannan electrospun membrane.

The invention discloses a biological catalytic synthesis method of norambrox ether, which comprises the following steps: step 1, starting from trans-nerolidol, carrying out carroll rearrangement reaction to synthesize farnesyl acetone; 2, in the presence of a cofactor, converting farnesyl acetone into high farnesol acetate under catalysis of Bayer-Villiger monooxygenase; step 3, catalyzing the deacetylation of the high-farnesol acetate by using esterase to obtain EE-high-farnesol; and step 4, carrying out enzymatic conversion on farnesol by using the squalene hopaene cyclase mutant to obtain norambrox ether. The transformation process is safe and non-toxic, Bayer-Villiger monooxygenase which is new with a key enzyme, natural co-expressed esterase, alcohol dehydrogenase and squalene and hopaene cyclase mutants matched with the system are used for synergistic transformation, so that not only is the coenzyme for oxidation reaction promoted to be recycled, but also the conversion efficiency is improved; conversion from farnesyl acetone to norambrox ether can be efficiently and specifically catalyzed, and a final product can be directly obtained from a reaction system without separation and purification of an intermediate.

The invention provides a polyarylester nanofiber and a preparation method thereof, and the preparation method comprises the following steps: firstly, carrying out acetylation reaction on 4-hydroxybenzoic acid (HBA) and 6-hydroxy-2-naphthoic acid (HNA) to obtain acetylated HBA and acetylated HNA, and then carrying out copolymerization on acetylated HBA and acetylated HNA and 4-hydroxybutyric acid (GHB) to obtain HBA / GSB / HNA copolymerized arylester granules B; the preparation method comprises the following steps: carrying out blending spinning on granules B and HBA / HNA copolymerized aromatic ester granules A obtained by copolymerizing acetylated HBA and acetylated HNA in the prior art, putting the obtained blended polyarylate fibers into an organic solvent containing a strong base, carrying out chemical etching and deprotonation, carrying out disassembly from top to bottom to obtain a polyarylate nanofiber suspension, and finally carrying out suction filtration and drying to obtain the polyarylate nanofibers. According to the preparation method disclosed by the invention, the blended polyarylester fiber containing an aliphatic chain is subjected to etching and deprotonation treatment through strong base to disassemble the polyarylester nanofiber, and a certain amount of-CH2-structure is introduced into a main chain, so that disassembly preparation of the nanofiber is facilitated, and the polyarylester nanofiber with uniform diameter and length and controllable length-diameter ratio is obtained.

The invention discloses an environment-friendly EVA (Ethylene Vinyl Acetate) material and belongs to the technical field of polymer composite materials. The environment-friendly EVA material is prepared from the following components: EVA resin, a bio-based dynamic cross-linking agent DBX-200, a natural fiber reinforced network, a hybrid flame-retardant filler, a bio-based plasticizer, zinc oxide, zinc stearate and an antioxidant. The bio-based dynamic cross-linking agent DBX-200 is introduced to replace a traditional toxic peroxide cross-linking system, pollution-free cross-linking is achieved, and the material is endowed with a self-repairing function. The natural fiber reinforced network is composed of silane coupling agent modified bamboo fibers and carboxylated nanocellulose, a multi-stage reinforced structure is formed, and the mechanical property is remarkably improved. The hybrid flame-retardant filler coats Mg (OH) 2 / HNTs through dopamine, so that synergistic flame retardance of a gas phase and a condensed phase is realized; the bio-based plasticizer is compounded by acetylated tributyl citrate and epoxidized cashew nut oil, and the plasticizing efficiency and the migration resistance are both considered. The material has the characteristics of high mechanical property, excellent flame retardance and environmental friendliness, and is suitable for the fields of shoemaking and the like.

The invention provides a full-degradable polymer composite material for tableware and a preparation method of the full-degradable polymer composite material, and belongs to the technical field of polymer materials. According to the invention, amylose corn starch, hydrophobic modified corn starch, cassava acetylated distarch phosphate, a polylactic acid-poly (butylene succinate) interpenetrating network structure material, a copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate, polycaprolactone, stearic acid modified micron titanium dioxide, chitosan quaternary ammonium salt and a composite plasticizer are adopted according to a specific ratio; the fully-degradable polymer composite material is obtained by compounding the composite heat stabilizer and stearic acid, has controllable and good biodegradability and good mechanical properties, enhances heat stability, improves processability, makes all the components easier to mix uniformly, facilitates molding processing of the material, and improves production efficiency and product quality.

The invention provides an acetylated surfactant capable of reducing the minimum miscible pressure of CO2 miscible displacement as well as a preparation method and application of the acetylated surfactant, and belongs to the technical field of CO2 oil displacement. The preparation method of the acetylated surfactant comprises the following steps: reacting a gluconic acid aqueous solution with n-caprylic alcohol under the action of a catalyst A to obtain n-octyl gluconate; under the action of a catalyst B, n-octyl gluconate and acetic anhydride react to obtain the acetylated surfactant for reducing the minimum miscible pressure of CO2 miscible flooding. The preparation method of the surfactant is simple, and the raw materials are cheap, wide in source, green and low in cost; the obtained surfactant can interact with CO2, has high CO2 affinity, and can effectively reduce the minimum miscible pressure of CO2 miscible flooding.

A method of treatment is disclosed, comprising administering a composition of Cyclodextrin and reduced, nanonized L-Glutathione to a patient in need of treatment, wherein the L-Glutathione molecule is non-acetylated, non-Esterified, and non-fatty acid attached.

The invention discloses a sodium alginate embedded iron-carbon microcapsule material for repairing cadmium and arsenic polluted soil and a preparation method of the sodium alginate embedded iron-carbon microcapsule material, and belongs to the technical field of soil heavy metal pollution repairing materials. The material comprises straw biochar, high-iron steel slag powder, a sulfydryl acetylated chitosan-iron monatomic cluster compound, a boron-doped graphitized carbon coated cobalt ferrite nanosheet, sodium alginate, calcium chloride and sodium carbonate. Wherein the sulfydryl acetylated chitosan is prepared by reacting chitosan with sulfydryl acetic anhydride, dialyzing and drying, and performing solvothermal reaction with ferric nitrate under high pressure. The preparation method comprises the following steps: pyrolyzing and drying the straw biochar, reacting the high-iron steel slag with sulfuric acid, adding the biochar, adjusting the pH value, adding sodium carbonate, mixing to obtain an iron-carbon-based passivation material, mixing with two modified compounds, mixing with a sodium alginate solution, dropwise adding a calcium chloride solution, and stirring and curing. The material can be applied to remediation of cadmium and arsenic polluted soil.

The application discloses an acyltransferase mutant with high acetylation activity and application thereof, and belongs to the technical field of enzyme engineering. The acyltransferase mutant provided by the application contains one or more mutation sites in the 62th and 64th positions on the basis of the acyltransferase mutant with the amino acid sequence shown in SEQ ID NO. 1. The acyltransferase mutant obtained by the application has higher acyltransferase activity and catalytic efficiency than the original enzyme before mutation, and can improve the yield of N-acetyl-trans-4-hydroxyproline. Therefore, the acyltransferase mutant of the application has important significance in the efficient biosynthesis of N-acetyl-trans-4-hydroxyproline, and has wide application prospects in the fields of biological materials, medicines or cosmetics.

The present invention relates to a high-temperature starch-type wetting agent and a preparation method and application thereof, wherein the wetting agent comprises an active ingredient and water, wherein the mass ratio of the active ingredient to the water is (0.05-0.15): 1, and the active ingredient comprises the following raw materials in parts by weight: 45-65 parts of a starch-based film-forming agent, 5-10 parts of a plasticizer, 15-20 parts of a lubricant, 1-2.5 parts of an emulsifier, 4-6 parts of a coupling agent, 0.5-1.5 parts of a preservative, and 0.02-0.05 parts of a defoaming agent; wherein the plasticizer is cardanol acetate, tributyl citrate, and mannitol in a mass ratio of 1:1:(1.5-2); and the starch-based film-forming agent is at least one of acetylated distarch adipate, oxidized starch, and hydroxypropyl starch. The glass fiber yarn products produced with the wetting agent have significantly reduced scattered silk defects on the cloth surface, and the amount of powder falling during the weaving process is also greatly reduced, which meets the high standard requirements for the production of printed circuit boards.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide cAMP-1116nc with a broad-spectrum antibacterial effect and application of the antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc is obtained by carrying out N-terminal acetylation modification and C-terminal amidation modification on a polypeptide as shown in SEQ ID NO. 1. The antibacterial peptide cAMP-1116nc is an antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc disclosed by the invention shows remarkable antibacterial activity in a plurality of gram-positive bacteria and gram-negative bacteria. The antibacterial peptide cAMP-1116nc can efficiently kill clinically common gram-negative pathogenic bacteria acinetobacter baumannii under the conditions that the pH is equal to 2, the pH is equal to 7.4 and the pH is equal to 8.4, has relatively low cytotoxicity, has the potential of preparing antibacterial drugs, and is suitable for developing antibacterial products with multiple purposes, including the antibacterial drugs, disinfectants and biological preservatives. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention discloses a lignin-based epoxy anticorrosive paint and a preparation method thereof. The anticorrosive paint is prepared by mixing a lignin-based epoxy anticorrosive paint component, a lignin-based phenolic amine curing agent and a diluent. According to the lignin-based epoxy anticorrosive paint disclosed by the invention, on one hand, a lignin epoxy resin acetylation modification method based on lipase is introduced, and the content of hydrophilic groups is reduced, so that formation of a compact lignin cross-linked network structure is promoted, and the barrier ability, heat resistance and aging resistance of an anticorrosive coating on a chemical medium are improved on the resin level; on the other hand, lignin and furfural are respectively used for partially replacing phenol and paraformaldehyde to prepare a lignin-based phenolic aldehyde amine curing agent with high chemical inertness, so that the protection effect of a coating in an extreme corrosion environment and the adhesive force to a metal base material are improved on the curing agent level. The lignin-based epoxy anticorrosive paint prepared by the invention has good corrosion resistance, aging resistance, thixotropy and adhesive force.

The disclosure relates, in part, to the use of compositions of poly N-acetylated glucosamine (PNAG), deacetylated PNAG (dPNAG), and antibodies and antibody fragments specific to PNAG in the prevention and treatment of filariasis.

The invention discloses a propolis extract-loaded sprayable bifunctional gel as well as a preparation method and application thereof. The preparation method comprises the following steps: synthesizing acetylated carboxymethyl chitosan by taking carboxymethyl chitosan and acetic anhydride as raw materials; the preparation method comprises the following steps: reacting acetylated carboxymethyl chitosan and aminophenylboronic acid serving as raw materials to synthesize a boric acid modified acetylated carboxymethyl chitosan gel precursor; the preparation method comprises the following steps: blending a boric acid modified acetylated carboxymethyl chitosan gel precursor with water, and self-assembling into A-CC-PBA hydrogel under the action of a hydrogen bond and a pi-pi bond; and adding the propolis extract into the A-CC-PBA hydrogel, so as to obtain the A-CC-PBA hydrogel. The gel prepared by the invention has good adhesion performance, can stably retain in the local part of the oral ulcer and can realize efficient targeted recognition and slow release of drugs to realize efficient sterilization and long-acting anti-inflammatory effects, the curative effect on the oral ulcer is obviously improved, and a new preparation type is provided for oral mucosa administration.

The invention discloses acyltransferase, a mutant and application in preparation of acetyl harpagide, the acyltransferase ancestor sequence capable of biologically catalyzing the acetylation of harpagide is firstly screened, then the acyltransferase is subjected to mutation modification, the enzyme activity is further improved, the harpagide is used as a substrate, and the acyltransferase and the mutant are synthesized into the acetyl harpagide. The ancestor acyltransferase and the mutant are used for acylation reaction, so that the application of the acyltransferase in the fields of medicines and the like is promoted, and certain help is provided for the aspect of drug acylation modification.

The invention belongs to the technical field of biology, and relates to a method for large-scale preparation of high-purity hydrolyzed corac acid or a salt thereof. The method comprises the following steps: 1) adding a solution containing corac acid or a salt thereof into a corac acid degrading enzyme containing an amino acid sequence as shown in SEQ ID NO: 1 or having at least 90% sequence identity with the SEQ ID NO: 1; terminating in the step 2) is achieved through a terminating agent, a boiling water bath and / or an ice bath, the terminating agent is selected from one or more of activated carbon, absolute ethyl alcohol, methyl alcohol, EDTA, trichloroacetic acid and sodium carbonate, and preferably, the activated carbon is used for achieving terminating in the step 2); and further obtaining the hydrolyzed corac acid or the salt thereof. The molecular structure of the hydrolyzed sodium currate prepared by the method provided by the invention has acetylation and pyruvic acid modification, the preparation condition is mild, the production cost is low, continuous production can be realized, and industrial amplification is easy.

Provided is method for producing a glycoside in which a phenolic compound is bonded to an anomeric position of a sugar, the method including: a step (1) of reacting a peracetylated sugar with the phenolic compound in the presence of an acid catalyst; and a step (2) of directly adding a deacetylating agent and a deacetylating solvent selected from the group consisting of water and an alcohol to a reaction mixture obtained in the step (1) to perform a deacetylation reaction.

A dPSA-binding agent conjugate, dPSA binding agent, and methods of use to treat cancer and kill cancer cells.

The invention discloses bioactive gel for accelerating wound healing. The gel is prepared from the following raw materials in percentage by weight: 0.8%-1% of bomer 980, 5%-5.5% of glycerol, 15%-20% of white vaseline, 51.7%-55% of phosphate buffer, 1.8%-2% of nano royal jelly acid, 2%-3% of recombinant III type human collagen, 0.4%-0.5% of sodium hyaluronate, 1%-3% of sodium alginate, 20%-25% of polyethylene glycol-400, 1.5%-3% of dopamine-methacrylamide copolymer, 0.5%-0.8% of nano silver and 0.3%-0.5% of phenoxyethanol. The nano royal jelly acid and the nano silver have a synergistic antibacterial effect, so that the inhibition effect on drug-resistant bacteria such as MRSA and pseudomonas aeruginosa is remarkably improved, and meanwhile, the wound epithelization can be accelerated and scar formation can be reduced through the dual effects of the recombinant III type collagen and the acetylated sodium hyaluronate. The defects that a traditional dressing is narrow in antibacterial spectrum, single in repairing effect, poor in environmental adaptability and the like are overcome, and the dressing is particularly suitable for complicated wounds such as diabetic foot ulcer and second-degree burn.

The invention relates to a preparation method of triethylene glycol diacetate, and belongs to the technical field of organic synthesis. The method comprises the following steps: adding triethylene glycol, an acetylation reagent and a multifunctional catalyst into a reaction kettle provided with a stirrer, a thermometer and a reflux condensing tube, and stirring and heating for reaction under the protection of nitrogen; after the reaction is finished, cooling reaction liquid to room temperature, and filtering and recovering the catalyst; performing reduced pressure distillation and silica gel column purification on the filtered reaction liquid to obtain triethylene glycol diacetate; the multifunctional catalyst is prepared from mesoporous silicon dioxide microspheres, 3-mercaptopropyltrimethoxysilane, 4-vinyl benzo-18-crown ether, sodium ethoxide, absolute ethyl alcohol, an ethanol solution of tetrabutyl titanate and chlorosulfonic acid. The preparation method comprises the following steps: preparing the mesoporous silicon dioxide microspheres, 3-mercaptopropyltrimethoxysilane, 4-vinyl benzo-18-crown ether, sodium ethoxide, absolute ethyl alcohol, the ethanol solution of tetrabutyl titanate and chlorosulfonic acid; the triethylene glycol diacetate prepared by the method has high yield and high purity, and can meet industrial production and market requirements.

The invention discloses a peracetylated polysaccharide derivative mixing accelerator as well as a preparation method and application thereof, and relates to the technical field of polysaccharide derivatives. Alkyl polyglucoside and glycidyl ether are subjected to etherification reaction to obtain polysaccharide derivatives; and adding acetic anhydride and an organic weak base catalyst into the polysaccharide derivative, and carrying out esterification reaction to obtain the peracetylated polysaccharide derivative. The obtained peracetylated polysaccharide derivative can be used for reducing the minimum miscible pressure of CO2 in an offshore oilfield. The polysaccharide derivative provided by the invention is simple in synthesis process, and the molecular activity of the polysaccharide derivative can be regulated and controlled by changing the number of carbon dioxide-philic groups and the length of oleophylic unsaturated hydrocarbon chains, so that the polysaccharide derivative can be used in the CO2 oil displacement process of oilfield exploitation, and the minimum miscible pressure in the CO2 oil displacement process is effectively reduced.

Disclosed herein is a modified ribonucleotide comprising a nucleoside comprising 2′-O-acetylated ribose, and polyribonucleotides comprising the same. Also provided herein are compositions comprising a polyribonucleotide of the present disclosure and methods of making and using the same.

The invention belongs to the field of liquid crystal polyarylester synthesis, and particularly relates to a liquid crystal polyarylester synthesis method and application of a high-boiling-point imidazole catalyst in synthesis of the liquid crystal polyarylester, and the synthesis method comprises the following steps: under the protection of nitrogen, mixing a monomer, an acylation reagent and a catalyst according to a certain proportion, heating, and carrying out acetylation and melt polycondensation to obtain a liquid crystal polyester melt; and cooling to obtain a liquid crystal polyarylester finished product. According to the liquid crystal polyester synthesis method provided by the invention, the specific high-boiling-point imidazole catalyst is selected and can be continuously and efficiently used for synthesizing liquid crystal polyarylester, so that acetylation reaction can be effectively catalyzed, melt polycondensation reaction can be continuously and efficiently catalyzed, the reaction time of polycondensation is greatly shortened, and side reactions are reduced.

The present invention provides a chemical synthesis method of EPS-G7, wherein β-cyclodextrin A is acetylated to obtain β-acetylcyclodextrin B, B is ring-opened in a mixed solution of acetic anhydride / concentrated sulfuric acid to obtain a chain acetylated heptose C, p-nitrophenol is condensed with intermediate C using boron trifluoride acetonitrile to obtain a key intermediate p-nitrophenyl (G1)-O-acetyl-D-maltoseptaglycoside D with α as the dominant configuration, D is removed from the acetyl group under the action of sodium methoxide to obtain pNP-G7 (p-nitrophenyl (G1)-D-maltoseptaglycoside), pNP-G7 is in the presence of p-toluenesulfonic acid and dimethoxyethane, and acetal protection is formed between the hydroxyl groups on C4 and C6 of the terminal monosaccharide to obtain EPS-G7. The reaction raw materials used in the method of the present invention are cheap and readily available, and the chemical synthesis of α-amylase detection reagent EPS-G7 is realized, which is a simple, economical and efficient synthesis method.

The invention discloses a galactose-masked aminoquinoline compound and application thereof, the structural formula of the galactose-masked aminoquinoline compound is # imgabs0, and R is acetyl, propionyl or butyryl. After being subjected to hydrolysis deacetylation, propionylation or butyrylation by esterase in cells, the compound can be activated by senescence-related beta galactosidase in senescence cell lysosome to release aminoquinoline (NCQ) so as to inhibit the survival of senescence cells in vivo or in vitro.

The invention discloses multifunctional nano-enzyme composite hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: synthesizing molybdenum disulfide MoS2 by taking hexaammonium heptamolybdate tetrahydrate and thiourea as raw materials; the preparation method comprises the following steps: carrying out stirring reaction on MoS2 and cysteine to obtain MoS2-LC; the preparation method comprises the following steps: mixing galangin with MoS2-LC, carrying out ultrasonic treatment, and carrying out a stirring reaction to obtain MoS2-LC (at) Gal; the preparation method comprises the following steps: adding acetic anhydride into a carboxymethyl chitosan aqueous solution to obtain N-acetylated carboxymethyl chitosan N-CC; the preparation method comprises the following steps: adding 1-butyl-3-methylimidazole chlorine salt into N-CC to react, and carrying out alcohol precipitation to obtain N-CC-IL; a cross-linking agent, MoS2-LC (at) Gal and N-CC-IL are subjected to a blending reaction, and SNCI (at) MLG is obtained. Pharmacodynamic results show that the multifunctional nano-enzyme composite hydrogel prepared by the preparation method disclosed by the invention can play a role in inhibiting ferroptosis while having an antibacterial effect, and a new possibility is provided for periodontal local treatment.

The invention provides application of a deacetylase inhibitor or a derivative thereof and application of a pharmaceutical composition, and belongs to the technical field of biology. The application of the deacetylase inhibitor or the pharmaceutically acceptable derivative thereof comprises the application of the deacetylase inhibitor for preparing a medicine for treating osteoporosis; wherein the deacetylase inhibitor is used for inducing histone acetylation in a cell nucleus. By inducing acetylation of histone in a cell nucleus, the differentiation direction of the bone marrow mesenchymal stem cells is accurately and efficiently controlled, and a treatment scheme for osteoporosis related diseases is developed.