851 results about "Galactosucrose" patented technology

The invention discloses a scalp essence capable of controlling oil and removing dandruff and a preparation method thereof, and provides the scalp essence capable of controlling oil and removing dandruff. The formula consists of the following raw materials: water, saccharomycetes / rice fermentation product filtrate, butanediol, phenoxyethanol, caprylic acid / capric triglyceride, galactose yeast-like bacterium fermentation product filtrate, allantoin, a saccharomycetes / zinc fermentation product, nicotinamide, arginine, polysorbate-20, hydrogenated lecithin, acrylic acid (ester) / C10-30 and an alkanol acrylate cross-linked polymer. Hydrolyzed sodium hyaluronate and trace components. According to the oil-controlling and dandruff-removing scalp essence disclosed by the invention, through the synergistic interaction among the raw materials, the effects of replenishing water, preserving moisture, controlling oil and removing dandruff are increased, the effects of relieving, recovering oil-water balance, recovering skin elasticity and delaying senescence are also achieved, the effects of preventing hair loss and nourishing hair are also achieved, and meanwhile, the oil-controlling and dandruff-removing scalp essence has the advantage of being stable in storage.

The invention provides modified acidic polysaccharide of lithocarpus litseifolius. The modified acidic polysaccharide is prepared from natural acidic polysaccharide of lithocarpus litseifolius through a chemical method or an enzyme method. Every 100 mg of the modified acidic polysaccharide of lithocarpus litseifolius contains 89.75 mg + / -1.04 mg to 90.67 mg + / -1.46 mg of total polysaccharide. The total uronic acid content is 23.70 mg + / -1.58 mg to 71.74 mg + / -2.07 mg; total bound phenol: 2.06 mg of GAE + / -0.06 mg of GAE-4.56 mg of GAE + / -0.04 mg of GAE (equivalent weight of gallic acid); the esterification degree is 3.72% + / -0.90% to 34.98% + / -0.62%; the molecular weight is (0.562 + / -0.012) * 10 < 4 > Da-(4.268 + / -0.055) * 10 < 4 > Da; the proportion of the galacturonic acid glycan (HG) is 15.28% to 65.02%; the ratio of the type I rhamnogalacturonan (RG-I) is 28.47% to 72.37%; and the length of a side chain is 0.44 to 10.31. The invention further provides a preparation method and application of the lithocarpus litseifolius modified acidic polysaccharide. The lithocarpus litseifolius modified acidic polysaccharide prepared by the invention has remarkable immunoregulatory activity, and especially has better immunocompetence when being used for removing arabinose side chains and reducing galactose side chains.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention relates to the technical field of biology, in particular to a beta-1, 4-galactosyl transferase mutant and a method for biologically synthesizing lactose-N-neotetraose by using the beta-1, 4-galactosyl transferase mutant. The invention discloses a protein, which is a protein HpgalT-186 or a protein HpgalT-146-186, a protein HpgalT-146 and a protein HpgalT-146. The protein HpgalT-186 is a protein obtained by mutating an amino acid residue at the 186th site of a wild type HpgalT; the protein HpgalT-146-186 is a protein obtained by carrying out mutation on an amino acid residue at the 186 site and an amino acid residue at the 146 site of wild type HpgalT. On the basis, a series of escherichia coli is constructed and optimized, the yield of the LNnT synthesized through shake flask fermentation of the finally obtained strain reaches 1.73-4.1 g / L, the highest yield in a 5L fermentation tank reaches 20-45.2 g / L, and efficient synthesis of the LNnT is achieved.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention discloses a chromogenic culture medium for detecting gram-negative bacteria in bovine mastitis and a test plate thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the compound chromogenic enzyme substrate is red tetrazole, 5-bromo-6-chloro-3-indole-beta-D-galactoside, and 5-bromo-4-chloro-3-indole-beta-D-glucopyranoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme substrate, and the compound chromogenic enzyme substrate is 5-bromo-6-chloro-3-indole-beta-D-glucopyranoside. The chromogenic culture medium and the test board prepared from the chromogenic culture medium can effectively inhibit the growth of non-gram negative bacteria, have the characteristics of strong specificity, high sensitivity, short detection period, easy observation and low detection cost, and have huge development potential and wide application prospects in microbiological detection.

The invention relates to the technical field of daily chemicals, in particular to a skin care matrix with skin micro-ecology regulating and hair loss preventing effects and cosmetics thereof. The invention discloses a skin care matrix with effects of regulating skin micro-ecology and preventing hair loss. Comprising the following components in parts by weight: 60 to 70 parts of galactose yeast-like bacterium fermentation product filtrate, 0.05 to 0.5 part of Chinese arborvitae twig and leaf extract, 0.05 to 0.5 part of citrus fruit extract, 0.05 to 0.5 part of white willow bark extract, 0.05 to 0.5 part of officinal magnolia bark extract, 0.05 to 0.5 part of lotus leaf extract, 0.05 to 0.5 part of sea buckthorn fruit extract, 0.05 to 0.1 part of ceramide NS / ceramide NG and 0.05 to 0.5 part of Italian helichrysum extract. 1.0 to 5.0 parts of lactobacillus / soybean extract fermentation product filtrate, 0.5 to 1.0 part of vitex trifolia fruit extract, 0.05 to 0.3 part of hydrolyzed keratin and 0.05 to 0.3 part of hydrolyzed vegetable protein; according to the skin care matrix, the microbial fermentation product and the plant extract are used as skin probiotics, and ceramide and other components are used as auxiliary materials to generate a synergistic effect, so that the skin care matrix shows excellent effects of balancing scalp micro-ecology, repairing hair follicles, refreshing and preventing hair loss on the premise of ensuring safety and mildness.

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The invention provides a composition combining probiotics and prebiotics as well as a preparation method and application of the composition, and relates to the technical field of biological medicines. The composition disclosed by the invention comprises the following components: compound probiotics and compound prebiotics, the compound probiotics are lactobacillus crispatus and plant lactobacillus in a mass ratio of (0.5-2): (3-8); the preservation number of the lactobacillus crispatus is CGMCC (China General Microbiological Culture Collection Center) No.26680, and the preservation number of the plant lactobacillus is CGMCC No.26679; the composite prebiotics are galactooligosaccharide, isomaltooligosaccharide, raffinose, spirulina and inulin, and the mass ratio of the galactooligosaccharide to the isomaltooligosaccharide to the raffinose to the spirulina to the inulin is (2.5-5): (2-4): (2-4): (0.5-3): (0.5-1.5). The prebiotics with specific components and proportions are compounded, so that the activity of the compound probiotics is protected, and the effects of treating inflammation and inhibiting harmful bacteria of vaginitis are improved.

The invention relates to a targeted drug delivery carrier based on mesoporous polydopamine nanoparticles and application thereof.The targeted drug delivery carrier is galactose modified mesoporous dopamine coordinated with manganese ions, lysine is adopted in the carrier synthesis step to provide an alkaline environment needed by dopamine polymerization, the polymerization process is simplified, and the drug delivery efficiency is improved. Meanwhile, lysine can be introduced into the mesoporous polydopamine; and the drug loading capacity of the carrier is improved. The carrier gathers multifunctionality of drug delivery, tumor imaging and treatment, provides a comprehensive new method for tumor treatment, and enhances individuation and precision of treatment.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The present invention the invention concerns bifunctional compounds of Formula (I) A-T-L1-AG, Formula (II) A-L1-Con(L2-AG)n or Formula (III) Ak´-Th-L3 ii-AGj' wherein A is a protein that binds to the hinge region of galactose deficient IgA1 antibodies or a protein that binds to autoantibodies of galactose deficient IgA antibodies and AG is an asiaglycoprotein receptor binding moiety (ASGPR binding moiety). L1, Con and L2 are part of a linker between A and AG. The A has the ability to bind to antibodies that are a cause of IgA nephropathy and the asiaglycoprotein receptor binding moiety binds to hepatic cells thereby initiating endocytosis and removal of the antibodies that are a cause of IgA nephropathy from the blood stream.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

The invention discloses a haemonchus contortus subunit vaccine, and belongs to the field of veterinary vaccines. The subunit vaccine comprises an antigen protein MEP3, an antigen protein AP1, an antigen protein AP8, an antigen protein H11-2, an antigen protein H11-4 and an antigen protein AP5 of galactosylated and modified haemonchus contortus, and the antigen protein MEP3, the antigen protein AP1, the antigen protein AP8, the antigen protein H11-2, the antigen protein H11-4 and the antigen protein AP5 of galactosylated and modified haemonchus contortus. The galactosylation modification of the antigen protein is realized by jointly expressing glycosyl transferase HcGALT of haemonchus contortus and one or more of the proteins in eukaryotic cells. The antigen protein modified by galactosylation in the subunit vaccine is closer to the glycosylation modification of natural protein, and can provide effective immune protection after the goat is infected with haemonchus contortus, and the egg laying rate is reduced by 81.43%. The invention lays a foundation for development of haemonchus contortus subunit vaccines, provides an effective technical means for prevention and treatment of the haemonchus contortus subunit vaccines, and has important application value and popularization prospect.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention provides a soluble dietary fiber composition. The soluble dietary fiber composition comprises galactomannan, kelp dietary fibers, oat beta glucan, galactooligosaccharide, polydextrose, stachyose and xanthophyll. The invention also provides application of the soluble dietary fiber composition in functional food or constipation treatment medicines.

The invention belongs to the technical field of skin care, and discloses a soothing and moisturizing composition, a preparation method, application and a skincare product, the soothing and moisturizing composition is obtained by taking jujube fruits, Chinese wolfberry fruits and centella as fermentation substrates and fermenting saccharomycetes and / or galactose yeast-like bacteria in the fermentation substrates, the weight ratio of the jujube fruits to the Chinese wolfberry fruits to the centella asiatica is (1-5): (2-8): (1-2). The soothing and moisturizing composition obtained by combined fermentation of the jujube fruits, the Chinese wolfberry fruits and the centella asiatica as fermentation substrates can generate a synergistic effect, so that the skin soothing, moisturizing and antioxidant effects of the soothing and moisturizing composition are improved.

The invention relates to recombinant escherichia coli and a method for synthesizing sialic acid lactose-N-tetrasaccharide c, and belongs to the technical field of microbial metabolism engineering. According to the research, engineering escherichia coli BAZ for producing LNTri II is taken as an initial strain, two key glycosyl transferases, namely beta 1, 4-galactosyl transferase and alpha 2, 6-sialyltransferase, and three CMP-Neu5Ac pathway enzymes, namely NeuC, NeuB and NeuA, are introduced, and related competitive genes are knocked out; under the conditions of shake-flask culture and fed batch culture, the LST c yield of 1.718 g / L and the LST c yield of 9.745 g / L are achieved through the engineering bacterium respectively, and the huge potential of a microbial cell factory in the aspect of efficient production of complex breast milk oligosaccharides is shown.

The invention discloses a method for improving in-vivo and in-vitro antioxidant activity of pomegranate peel polyphenol extract through combined enzymolysis of tannase and beta-glucosidase, and relates to the technical field of food science and biology. The pomegranate peel polyphenol extract provided by the invention is obtained from pomegranate peel powder through solvent extraction. According to the present invention, tannase and beta-glucosidase are adopted to perform single or combined enzymolysis on the pomegranate peel polyphenol extract, and the determination on the in vitro antioxidant activity of the pomegranate peel polyphenol extract shows that the antioxidant activities of DPPH and ABTS after the combined enzymolysis are improved by 29.84% and 29.30% compared with the antioxidant activities of the untreated polyphenol extract; the UPLC-ESI-QTOF-MS is used for identifying and analyzing the components of the enzymatic hydrolysate, the content of macromolecular ellagic tannin is obviously reduced, and the content of micromolecular ellagic acid and gallic acid is increased. The enzymolysis polyphenol is applied to D-galactose induced oxidative stress mice, and the result shows that the oxidative damage relieving effect of the enzymolysis polyphenol on the mice is better than that of untreated polyphenol.

The invention discloses a prebiotic composition capable of proliferating bifidobacterium and application of the prebiotic composition. The prebiotic composition comprises polydextrose, isomaltooligosacharide and galactooligosaccharide. Wherein the mass ratio of the polydextrose to the isomaltooligosacharide to the galactooligosaccharide is (0-2): (3-5): (4-5). The prebiotic composition provided by the invention can increase the content of total bifidobacteria in intestinal tracts, especially can specifically proliferate bifidobacteria longum and bifidobacteria breve, and can accurately regulate and control intestinal flora, thereby accurately intervening in host health.

The present disclosure is directed to human IgE antibodies and fragments thereof binding to galactose-α-1,3-galactose, a known red meat allergen, and methods for use thereof.

Provided herein are compositions, methods, and uses involving antibodies that immunospecifically bind the Galactin-3 (LGALS3) carbohydrate binding domain (CBD). Also provided herein are uses and methods for managing, treating, or preventing disorders, such as cancer.

The invention discloses a composition for resisting porcine viral diarrhea and application of the composition. The composition for resisting porcine viral diarrhea is prepared from the following components in parts by mass: 2 parts of phloretin, 1 part of N-acetyl-D-galactosamine and 100 parts of proline. The composition can further comprise one or more auxiliary materials of absolute ethyl alcohol, propylene glycol, dimethyl sulfoxide, edible oil, water, tween-80, lactose, defatted rice bran, corncob powder, soluble starch, bran, silicon dioxide, montmorillonite, vermiculite, medical stone, zeolite powder, white carbon black and the like. The composition can significantly inhibit the proliferation of PEDV, TGEV and PoRV, and inhibit the infection of PEDV, TGEV and PoRV on cells, and further, the composition can be used for preparing drugs or feed additives for resisting porcine diarrhea diseases caused by viruses such as PEDV, TGEV and PoRV.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses a composition for proliferating animal bifidobacterium subsp. Lactis and application of the composition, and belongs to the field of probiotics, and the composition comprises a prebiotic composition and a traditional Chinese medicine composition, wherein the mass ratio of the prebiotic composition to the traditional Chinese medicine composition is (5-10): (1-5); the prebiotic composition comprises at least three of mannose oligomer, cotton seed oligosaccharide, isomaltooligosaccharide and galactooligosaccharide; the traditional Chinese medicine composition comprises a Chinese prescription and ginseng powder, and the Chinese prescription comprises at least two of astragalus membranaceus, liquorice and ginger and cinnamon. The composition capable of efficiently proliferating the animal bifidobacterium subsp.lactis is screened out, the proliferation speed and the proliferation amount are both considered, the probiotic effect of probiotics is enhanced, the composition can help proliferation of the animal bifidobacterium subsp.lactis, the advantages of rapidness and high growth are both achieved, and more health effects are achieved.

The invention relates to a three-cluster galactose compound, a conjugate as well as a preparation method and application of the three-cluster galactose compound. Specifically, the invention relates to compounds of formula (I) and conjugates of the compounds and drug molecules. The invention also relates to a preparation method of the compound, and application of the compound and the conjugate in preparation of drugs for treating or preventing diseases related to expression or overexpression of genes in hepatocytes. # imgabs0 #

The invention belongs to the technical field of analysis and detection, and particularly relates to a beta-galactosidase response type hemicyanine near-infrared fluorescent probe, a preparation method thereof and application of the beta-galactosidase response type hemicyanine near-infrared fluorescent probe as a tumor organ imaging probe. The near-infrared fluorescent probe provided by the invention takes a water-soluble hemicyanine skeleton as a fluorophore, so that the biocompatibility of the near-infrared fluorescent probe is improved; based on the principle that beta-galactose can be selectively cut by beta-galactosidase, a galactose structure with a self-leaving group is used as a response group, selective quick response to the beta-galactosidase is achieved, and high sensitivity to the beta-galactosidase is shown. The near-infrared fluorescent probe provided by the invention can emit near-infrared fluorescence with long wavelength, has lower background fluorescence and good penetration depth, and can be directly used for detecting organoids containing the matrigel without eliminating the matrigel, so that the organoids are prevented from being damaged or additional experimental variables are prevented from being introduced in the process of eliminating the matrigel.

The invention provides a genetically engineered bacterium for producing lactose-N-neotetraose as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium meets the following conditions: (1) exogenous expression of an MFS transport protein, beta-1, 3-N-acetylglucosamine transferase and beta-1, 4-galactosyl transferase is carried out; and (2) over-expression of a galactoside osmose enzyme and a UDP-galactose-4-epimerase is carried out. According to the genetically engineered bacterium disclosed by the invention, the MFS transporter, the beta-1, 3-N-acetylglucosamine transferase and the beta-1, 4-galactosyl transferase are exogenously introduced, and the expression quantity of galactosidase and UDP-galactose-4-epimerase in a genome is enhanced, so that the residual quantity of LNT II in an LNnT synthesis route can be effectively reduced, and the fermentation yield of the LNnT is increased.

The invention discloses a galactose-deficient IgA1 specific antibody or an antigen binding fragment thereof, and a related biological material and application thereof, and belongs to the technical field of antibodies. The main problem to be solved in the invention is how to obtain the anti-Gd-IgA1 antibody with high specificity. In order to solve the technical problem, the invention provides the antibody or the antigen binding fragment thereof, and the antibody or the antigen binding fragment thereof comprises a heavy chain variable region as shown in the 1st-122nd sites of SEQ ID NO: 1 and a light chain variable region as shown in the 1st-109th sites of SEQ ID NO: 2. Compared with the existing antibody KM55, the obtained antibody has the advantage of high antigen affinity, and is expected to be more effectively used for diagnosis, treatment effect monitoring, prognosis judgment and the like of IgA nephropathy.