Skin care matrix with effects of regulating skin micro-ecology and preventing hair loss and cosmetics thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a skin care matrix with skin micro-ecology regulating and hair loss preventing effects and cosmetics thereof. The invention discloses a skin care matrix with effects of regulating skin micro-ecology and preventing hair loss. Comprising the following components in parts by weight: 60 to 70 parts of galactose yeast-like bacterium fermentation product filtrate, 0.05 to 0.5 part of Chinese arborvitae twig and leaf extract, 0.05 to 0.5 part of citrus fruit extract, 0.05 to 0.5 part of white willow bark extract, 0.05 to 0.5 part of officinal magnolia bark extract, 0.05 to 0.5 part of lotus leaf extract, 0.05 to 0.5 part of sea buckthorn fruit extract, 0.05 to 0.1 part of ceramide NS / ceramide NG and 0.05 to 0.5 part of Italian helichrysum extract. 1.0 to 5.0 parts of lactobacillus / soybean extract fermentation product filtrate, 0.5 to 1.0 part of vitex trifolia fruit extract, 0.05 to 0.3 part of hydrolyzed keratin and 0.05 to 0.3 part of hydrolyzed vegetable protein; according to the skin care matrix, the microbial fermentation product and the plant extract are used as skin probiotics, and ceramide and other components are used as auxiliary materials to generate a synergistic effect, so that the skin care matrix shows excellent effects of balancing scalp micro-ecology, repairing hair follicles, refreshing and preventing hair loss on the premise of ensuring safety and mildness.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, in particular to a skin care matrix and cosmetics thereof having the functions of regulating skin microecology and preventing skin loss. Background Art

[0002] Hair loss refers to the phenomenon of hair loss. Normally shed hair is in the catagen and telogen phases. Since the hair entering the catagen and the newly entering the growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. Pathological hair loss refers to abnormal or excessive hair loss. Common causes include bacterial infection of hair follicles, inflamed and damaged hair follicles, and dysbiosis of the microecology. Traditional technology generally uses chemical reagents such as ketoconazole, salicylic acid, and selenium disulfide to inhibit microorganisms and inflammation, thereby achieving an anti-hair loss effect. However, in recent years, people have put forward higher requirements for health and environmental protection, and some products have begun to use natural ingredients to achieve anti-hair loss effects.

[0003] For example, a Chinese patent discloses a natural anti-hair loss composition, a preparation method and an application thereof, with application publication number 202310655302.1, and its specification records: a natural anti-hair loss composition and a preparation method thereof, the effective ingredients of the composition comprising nine natural extract components: Polygonum multiflorum, Angelica sinensis, Ligustrum lucidum, Drynaria fortunei, ginger, Platycladus orientalis leaves, Nasturtium, capsicum and citrus extracts; the preparation method of the nine natural extract components obtains Polygonum multiflorum, Angelica sinensis, Ligustrum lucidum, Drynaria fortunei, ginger, Platycladus orientalis leaves, Nasturtium, capsicum and citrus by different physical or chemical extraction and purification methods; the natural anti-hair loss composition of the present invention can be added to conventional scalp care ingredients or products, and significantly exerts its effects of inhibiting the reproduction of microorganisms, repairing damaged hair follicles, and reducing hair loss.

[0004] The above technical solution uses Platycladus orientalis to remove dandruff and promote hair growth and inhibit bacteria, and then uses citrus to strengthen the nutritional supply of hair roots and has a certain anti-dandruff effect, and finally strengthens the nutritional supply of hair follicles to repair hair follicles. The shortcomings of the existing technology are that it only focuses on hair roots and hair follicles, while ignoring the repair of the scalp. Obviously, when the scalp is damaged, it will inevitably be difficult to transport sufficient nutrients to the hair roots and hair follicles. In addition, when the skin is damaged, it will inevitably be more susceptible to microbial invasion. Summary of the invention

[0005] In view of the shortcomings of the prior art, one of the objects of the present invention is to provide a skin care matrix with the function of regulating skin microecology and preventing hair loss. The second object of the present invention is to provide a cosmetic containing a skin care matrix with the function of regulating skin microecology and preventing hair loss. The third object of the present invention is to provide a method for preparing a cosmetic containing a skin care matrix with the function of regulating skin microecology and preventing hair loss.

[0006] One of the purposes of the present invention is achieved through the following technical solutions:

[0007] A skin care matrix with the function of regulating skin microecology and preventing skin loss, comprising the following components in parts by weight:

[0008] Active ingredient A: 60-70 parts of filtrate of galactosyl yeast fermentation product;

[0009] Active ingredient B: 0.05-0.5 parts of Thuja orientalis leaf extract, 0.05-0.5 parts of citrus fruit extract, 0.05-0.5 parts of Salix alba bark extract, 0.05-0.5 parts of Magnolia officinalis bark extract, 0.05-0.5 parts of Nelumbo nucifera leaf extract, 0.05-0.5 parts of Hippophae rhamnoides fruit extract, ceramide NS / ceramide NG 0.05-0.1 parts, HELICHRYSUMITALICUM extract 0.05-0.5 parts, Lactobacillus / soybean extract fermentation product filtrate 1.0-5.0 parts, VITEXTRIFOLIA fruit extract 0.5-1.0 parts, hydrolyzed keratin 0.05-0.3 parts, hydrolyzed vegetable protein 0.05-0.3 parts.

[0010] Specifically, the preparation method of the lotus leaf extract comprises the following steps:

[0011] First, the mature lotus leaves were washed and immersed in an enzyme solution for pretreatment for 4 hours. The enzyme solution was a mixture of lipase powder and water in a weight ratio of 0.5:99.5, with a pretreatment temperature of 60°C, pH 8.0, and a stirring rate of 50 rpm.

[0012] Then the lotus leaves were taken out, rinsed, drained, crushed and air-dried at room temperature (25°C, the same below) to a water content of 20wt%;

[0013] Finally, the lotus leaf powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the lotus leaf extract.

[0014] Specifically, the preparation method of the Italian immortelle extract comprises the following steps:

[0015] First, the mature Italian immortelle plants (roots removed, the same below) were washed and immersed in an enzyme solution for pretreatment for 2 hours. The enzyme solution was a mixture of lipase powder, water and alkyl glycoside in a weight ratio of 1:98.7:0.3, with a pretreatment temperature of 60°C, pH 8.0 and a stirring rate of 150 rpm;

[0016] Then, the Italian immortelle plants were taken out, rinsed, drained, crushed, and air-dried at room temperature until the water content reached 20 wt %;

[0017] Finally, the Italian Immortelle plant powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the Italian Immortelle plant extract.

[0018] The second object of the present invention is achieved by the following technical solutions:

[0019] A cosmetic containing a skin care matrix having the function of regulating skin microecology and preventing skin loss, comprising the following components in parts by weight:

[0020] Skin care base 62-77.2 parts, guar hydroxypropyltrimonium chloride 0.2-0.3 parts, sodium laureth sulfate 13-16 parts, sodium lauroyl sarcosinate 1.5-3.0 parts, sodium lauroyl glutamate 1.5-3.0 parts, ammonium lauryl sulfate 1.5-2.0 parts, sodium lauryl sulfate 0.3-0.5 parts, cocamide methyl MEA 1.0-1.5 parts, glycerin 0.5-1 parts, allantoin 0.2-0.3 parts, citric acid 0.1-0.15 parts, dichlorobenzyl alcohol 0.1-0.11 parts, sodium isostearyl lactylate 0.05-0.1 parts, cocamidopropyl betaine 3.0-5.0 parts, menthol 0.2-0.25 parts, piroctone olamine salt 0.08-0.1 parts, flavor (daily use) 0.5-0.8 parts, cocamidopropyl PG-dimethyl ammonium chloride 0.5-0.8 parts, PPG-3 caprylyl ether 0.5-1.0 parts, phenoxyethanol 0.4-0.5 parts, sodium benzoate 0.2-0.4 parts, cocodimonium hydroxypropyl hydrolyzed wheat protein 0.2-0.3 parts and polyester-11 0.11-0.15 parts.

[0021] The third object of the present invention is achieved by the following technical solutions:

[0022] A method for preparing a cosmetic containing a skin care matrix having the function of regulating skin microecology and preventing skin loss, comprising the following steps:

[0023] Step 1, weighing the formula components, mixing the active component A in the skin care matrix with guar gum hydroxypropyltrimethylammonium chloride to obtain a first group of materials, mixing sodium laureth sulfate, sodium lauroyl sarcosinate, sodium lauroyl glutamate, ammonium lauryl sulfate, sodium lauryl sulfate, cocamide methyl MEA, glycerin, allantoin, citric acid, dichlorobenzyl alcohol and sodium isostearyl lactylate to obtain a second group of materials, mixing cocamidopropyl betaine, menthol and piroctone olamine salt to obtain a third group of materials, and mixing the active component B in the skin care matrix with essence, cocamidopropyl PG-dimethyl ammonium chloride, PPG-3 octyl ether, phenoxyethanol, sodium benzoate, cocodimonium hydroxypropyl hydrolyzed wheat protein and polyester-11 to obtain a fourth group of materials;

[0024] Step 2, adding the first group of materials during stirring, and then homogenizing to obtain a first slurry;

[0025] Step 3, stirring the first slurry and heating it, adding the second group of materials during stirring, and obtaining the second slurry after keeping the temperature;

[0026] Step 4, cooling the second slurry, adding the third group of materials during cooling, and stirring to obtain a third slurry;

[0027] Step 5, cooling the third slurry to 45° C., adding the fourth group of materials, and stirring to obtain a fourth slurry;

[0028] Step 6, stirring the fourth slurry for the first time and cooling it to 30-40° C., and then stirring it for the second time before discharging.

[0029] Specifically, in step 2, the stirring temperature is 25-35° C., the stirring frequency is 60-80 Hz, and the stirring time is 5 min; the homogenizing speed is 1800-2000 rpm, and the homogenizing time is 3-5 min.

[0030] Specifically, in step 3, the stirring frequency is 200-300 Hz, the temperature is raised to 80-85° C., and the insulation time is 30 minutes.

[0031] Specifically, in step 4, the stirring frequency is 60-80 Hz and the stirring time is 10-15 min.

[0032] Specifically, in step 5, the stirring frequency is 60-80 Hz and the stirring time is 10-15 min.

[0033] Specifically, in step 6, the first stirring speed is 30-60 rpm, and the temperature is lowered to 30-40° C.; the second stirring speed is 30-60 rpm, and the stirring time is 5-30 min.

[0034] The beneficial effects of the present invention are as follows: the skin care matrix uses microbial fermentation products and plant extracts as skin probiotics, supplemented by ceramide and other ingredients to produce a synergistic effect, while ensuring safety and mildness, and exhibits excellent effects of balancing scalp microecology, repairing hair follicles, revitalizing and preventing hair loss. DETAILED DESCRIPTION

[0035] The following is further described in conjunction with specific implementation methods:

[0036] Table 1 Example formula table

[0037]

[0038]

[0039]

[0040] The filtrate of the fermentation product of galactosyl yeast-like bacteria is prepared by conventional methods, and the lotus leaf extract and the Italian immortelle extract are prepared by conventional methods as follows. The remaining components are conventional raw materials in the field and are purchased through normal channels.

[0041] The preparation method of the lotus leaf extract comprises the following steps:

[0042] First, the mature lotus leaves were washed and immersed in an enzyme solution for pretreatment for 4 hours. The enzyme solution was a mixture of lipase powder and water in a weight ratio of 0.5:99.5, with a pretreatment temperature of 60°C, pH 8.0, and a stirring rate of 50 rpm.

[0043] Then the lotus leaves were taken out, rinsed, drained, crushed and air-dried at room temperature (25°C, the same below) to a water content of 20wt%;

[0044] Finally, the lotus leaf powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the lotus leaf extract.

[0045] The preparation method of the Italian immortelle extract comprises the following steps:

[0046] First, the mature Italian immortelle plants (roots removed, the same below) were washed and immersed in an enzyme solution for pretreatment for 2 hours. The enzyme solution was a mixture of lipase powder, water and alkyl glycoside in a weight ratio of 1:98.7:0.3, with a pretreatment temperature of 60°C, pH 8.0 and a stirring rate of 150 rpm;

[0047] Then, the Italian immortelle plants were taken out, rinsed, drained, crushed, and air-dried at room temperature until the water content was 20 wt %;

[0048] Finally, the Italian Immortelle plant powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the Italian Immortelle plant extract.

[0049] Example 1

[0050] The preparation of cosmetics containing a skin care base having the function of regulating skin microecology and preventing skin loss comprises the following steps:

[0051] Step 1, weighing the formula components, mixing the active component A (galactosyeast fermentation product filtrate and guar hydroxypropyl trimethyl ammonium chloride) in the skin care matrix with guar hydroxypropyl trimethyl ammonium chloride to obtain a first group of materials, sodium laureth sulfate, sodium lauroyl sarcosinate, sodium lauroyl glutamate, ammonium lauryl sulfate, sodium lauryl sulfate, cocamide methyl MEA, glycerin, allantoin, citric acid, dichlorobenzyl alcohol and sodium isostearyl lactylate to obtain a second group of materials, and mixing cocamidopropyl betaine, menthol and piroctone olamine salt to obtain a third group of materials. There are three groups of materials, and the active component B in the skin care matrix (Platycladus orientalis leaf extract, citrus fruit extract, white willow bark extract, Magnolia officinalis bark extract, lotus leaf extract, sea buckthorn fruit extract, ceramide NS / ceramide NG, Helichrysum italiensis extract, lactobacillus / soybean extract fermentation product filtrate, Vitex crenata fruit extract, hydrolyzed keratin and hydrolyzed vegetable protein) is mixed with essence, cocamidopropyl PG-dimonium chloride, PPG-3 caprylyl ether, phenoxyethanol, sodium benzoate, cocodimonium hydroxypropyl hydrolyzed wheat protein and polyester-11 to obtain the fourth group of materials.

[0052] Step 2, adding the first group of materials during stirring, with a stirring temperature of 25° C., a stirring frequency of 60 Hz, and a stirring time of 5 min; and then homogenizing to obtain the first slurry, with a homogenizing speed of 1800 rpm and a homogenizing time of 3 min.

[0053] Step 3, stirring the first slurry and heating it up, adding the second group of materials during stirring, stirring at a frequency of 200 Hz, and heating to 80° C.; obtaining the second slurry after heat preservation, and the heat preservation time is 30 minutes.

[0054] Step 4, cooling the second slurry, adding the third group of materials during cooling, and stirring to obtain the third slurry, with a stirring frequency of 60 Hz and a stirring time of 10 min.

[0055] Step 5, after cooling the third slurry to 45° C., add the fourth group of materials and stir evenly to obtain the fourth slurry, with a stirring frequency of 60 Hz and a stirring time of 10 min.

[0056] Step 6, stir the fourth slurry for the first time and cool it down to 30°C, the first stirring speed is 30rpm, and the temperature is cooled to 30°C; then stir it for the second time and discharge it, the second stirring speed is 30rpm, and the stirring time is 5min.

[0057] Example 2

[0058] The preparation of cosmetics containing a skin care base having the function of regulating skin microecology and preventing skin loss comprises the following steps:

[0059] Step 1, weighing the formula components, mixing the active component A (galactosyeast fermentation product filtrate and guar hydroxypropyl trimethyl ammonium chloride) in the skin care matrix with guar hydroxypropyl trimethyl ammonium chloride to obtain a first group of materials, sodium laureth sulfate, sodium lauroyl sarcosinate, sodium lauroyl glutamate, ammonium lauryl sulfate, sodium lauryl sulfate, cocamide methyl MEA, glycerin, allantoin, citric acid, dichlorobenzyl alcohol and sodium isostearyl lactylate to obtain a second group of materials, and mixing cocamidopropyl betaine, menthol and piroctone olamine salt to obtain a third group of materials. There are three groups of materials, and the active component B in the skin care matrix (Platycladus orientalis leaf extract, citrus fruit extract, white willow bark extract, Magnolia officinalis bark extract, lotus leaf extract, sea buckthorn fruit extract, ceramide NS / ceramide NG, Helichrysum italiensis extract, lactobacillus / soybean extract fermentation product filtrate, Vitex crenata fruit extract, hydrolyzed keratin and hydrolyzed vegetable protein) is mixed with essence, cocamidopropyl PG-dimonium chloride, PPG-3 caprylyl ether, phenoxyethanol, sodium benzoate, cocodimonium hydroxypropyl hydrolyzed wheat protein and polyester-11 to obtain the fourth group of materials.

[0060] Step 2, adding the first group of materials during stirring, with a stirring temperature of 30°C, a stirring frequency of 70HZ, and a stirring time of 5min; and then homogenizing to obtain the first slurry, with a homogenizing speed of 1900rpm and a homogenizing time of 4min.

[0061] Step 3, stirring the first slurry and heating it up, adding the second group of materials during stirring, stirring at a frequency of 250 Hz, and heating to 82° C.; obtaining the second slurry after heat preservation, and the heat preservation time is 30 minutes.

[0062] Step 4, cooling the second slurry, adding the third group of materials during cooling, and stirring to obtain the third slurry, with a stirring frequency of 70 Hz and a stirring time of 12 min.

[0063] Step 5, after cooling the third slurry to 45° C., add the fourth group of materials and stir evenly to obtain the fourth slurry, with a stirring frequency of 70 Hz and a stirring time of 12 min.

[0064] Step 6, stir the fourth slurry for the first time and cool it to 35°C, the first stirring speed is 45 rpm, and the temperature is cooled to 35°C; then stir it for the second time and discharge it, the second stirring speed is 45 rpm, and the stirring time is 15 minutes.

[0065] Example 3

[0066] The preparation of cosmetics containing a skin care base having the function of regulating skin microecology and preventing skin loss comprises the following steps:

[0067] Step 1, weighing the formula components, mixing the active component A (galactosyeast fermentation product filtrate and guar hydroxypropyl trimethyl ammonium chloride) in the skin care matrix with guar hydroxypropyl trimethyl ammonium chloride to obtain a first group of materials, sodium laureth sulfate, sodium lauroyl sarcosinate, sodium lauroyl glutamate, ammonium lauryl sulfate, sodium lauryl sulfate, cocamide methyl MEA, glycerin, allantoin, citric acid, dichlorobenzyl alcohol and sodium isostearyl lactylate to obtain a second group of materials, and mixing cocamidopropyl betaine, menthol and piroctone olamine salt to obtain a third group of materials. There are three groups of materials, and the active component B in the skin care matrix (Platycladus orientalis leaf extract, citrus fruit extract, white willow bark extract, Magnolia officinalis bark extract, lotus leaf extract, sea buckthorn fruit extract, ceramide NS / ceramide NG, Helichrysum italiensis extract, lactobacillus / soybean extract fermentation product filtrate, Vitex crenata fruit extract, hydrolyzed keratin and hydrolyzed vegetable protein) is mixed with essence, cocamidopropyl PG-dimonium chloride, PPG-3 caprylyl ether, phenoxyethanol, sodium benzoate, cocodimonium hydroxypropyl hydrolyzed wheat protein and polyester-11 to obtain the fourth group of materials.

[0068] Step 2, adding the first group of materials during stirring, with a stirring temperature of 35° C., a stirring frequency of 80 Hz, and a stirring time of 5 min; and then homogenizing to obtain the first slurry, with a homogenizing speed of 2000 rpm and a homogenizing time of 5 min.

[0069] Step 3, stirring the first slurry and heating it up, adding the second group of materials during stirring, stirring at a frequency of 300 Hz, and heating to 85° C.; obtaining the second slurry after heat preservation, and the heat preservation time is 30 minutes.

[0070] Step 4, cooling the second slurry, adding the third group of materials during cooling, and stirring to obtain the third slurry, with a stirring frequency of 80 Hz and a stirring time of 15 min.

[0071] Step 5, after cooling the third slurry to 45° C., add the fourth group of materials and stir evenly to obtain the fourth slurry, with a stirring frequency of 80 Hz and a stirring time of 15 min.

[0072] Step 6, stir the fourth slurry for the first time and cool it to 40°C, the first stirring speed is 60rpm, and the temperature is cooled to 40°C; then stir it for the second time and discharge it, the second stirring speed is 60rpm, and the stirring time is 30min.

[0073] Comparative Example 1

[0074] The lotus leaf extract is replaced by lotus leaf broken material, which is lotus leaf and water in a weight ratio of 1:10 put into a wall breaking machine, broken at a speed of 20000 rpm, and then passed through a 100 mesh sieve after standing for 4 hours, and the filtrate is air-dried at room temperature to a water content of 20wt%.

[0075] The remaining steps are the same as those in Example 2.

[0076] Comparative Example 2

[0077] The Italian immortelle extract is replaced by Italian immortelle broken wall material, wherein the Italian immortelle broken wall material is Italian immortelle plant and water in a weight ratio of 1:10, put into a wall breaking machine, broken at a speed of 20000 rpm, left to stand for 4 hours, and then passed through a 100 mesh sieve, and the filtrate is air-dried at room temperature to a water content of 20wt%.

[0078] The remaining steps are the same as those in Example 2.

[0079] Comparative Example 3

[0080] The lotus leaf extract and the Italian immortelle extract are both replaced with deionized water.

[0081] The remaining steps are the same as those in Example 2.

[0082] Comparative Example 4

[0083] Weigh the components of the formula and put them all into the emulsifier. Stir at a frequency of 70HZ for 5 minutes at a temperature of 30℃; then homogenize at a speed of 1900rpm for 4 minutes; then stir at a frequency of 200-300HZ and heat to 82℃, and keep warm for 30 minutes; stir at a frequency of 70HZ and cool to 45℃ and continue stirring for 12 minutes; stir at a speed of 45rpm and cool to 35℃; finally stir at a speed of 45rpm for 15 minutes and discharge.

[0084] Hair growth experiment

[0085] The purpose of the experiment is to verify that the present invention has a promoting effect on hair growth.

[0086] Experimental method: 9 adult rats were selected, 8 1cm*1cm areas were randomly selected from the back after hair removal, and 0.2mL of each of Examples 1-3, Comparative Examples 1-4 and tap water were smeared respectively. After 20 days, 9 hairs were randomly selected from each area to measure the length and take the average value.

[0087] The experimental results are shown in Table 2.

[0088] Table 2 Hair length record

[0089]

[0090] Conclusion, Examples 1-3 make the growth rate of mouse hair much higher than that of using tap water, that is, the present invention has the effect of promoting hair growth, and then has the effect of preventing hair loss. In addition, the experiment proves that when either lotus leaf extract or Italian helichrysum extract is missing, its therapeutic effect is almost the same as that of Comparative Example 3 without adding either of them, so they must have a synergistic effect. Comparative Example 4 proves that the preparation process can have a decisive influence on the therapeutic effect.

[0091] Antibacterial experiment

[0092] The purpose of the experiment is to verify that the present invention has an inhibitory effect on bacteria.

[0093] Experimental method: Take 0.3 mL of each of Examples 1-3, apply it on pig skin (cut into 4 cm*4 cm after sterilization), rinse with water for 2 minutes after 2 minutes, and then air-dry in a clean bench until there are no water droplets. Place the pig skin on a contaminated plate (LN plate, add 100 μL of 3×105 CFU / mL Malassezia suspension), take away the pig skin after 20 minutes and culture the plate at 32°C for 2 days, and calculate the inhibition rate.

[0094] The experimental results are shown in Table 3.

[0095] Table 3 Antibacterial rate statistics

[0096] sample Example 1 Example 2 Example 3 Antibacterial rate (%) 97.7 98.1 98.0

[0097] Conclusion: The antibacterial rate of all embodiments of the present invention after 48 hours is over 97%, indicating that they have excellent antibacterial performance.

[0098] Repair experiment

[0099] The purpose of the experiment is to verify that the present invention has a repairing effect on the skin.

[0100] Experimental method: A9 mouse subcutaneous connective tissue cells (purchased from Henan Industrial Microbial Strain Engineering Technology Research Center) were diluted with cell culture medium and cultured to a density of 50%, inoculated into 96-well plates, with a liquid volume of 200 μL per well, and placed in a CO2 incubator for 24 hours. The culture medium in the 96-well plate was discarded and the administration operation was carried out. A culture medium (composition content of 1.25 mg / mL) containing the test substance (one of the compositions obtained in Examples 1-3) was added to the test substance well, and normal cell culture medium was added to the blank / solvent control well, 200 μL per well. After the administration is completed, the 96-well plate is placed in a CO2 incubator for 24 hours. After the incubation and culture are completed, 200 μL of cell culture supernatant is collected in a 1.5 mL sterile centrifuge tube and placed in a -80°C ultra-low temperature refrigerator for freezing. Detection was performed according to the instruction manual of the mouse hyaluronic acid (HA) ELISA kit. Calculate the upregulation rate of hyaluronic acid expression.

[0101] The experimental results are shown in Table 4.

[0102] Table 4 Repair effect statistics

[0103] sample Example 1 Example 2 Example 3 Hyaluronic acid expression up-regulation rate (%) 6.78 6.81 7.09

[0104] In conclusion, the present invention can significantly increase the expression of hyaluronic acid in the skin, thus having a repairing effect on the scalp.

[0105] The above embodiments and descriptions are only for illustrating the principles and best embodiments of the present invention. Without departing from the spirit and scope of the present invention, the present invention may be subject to various changes and improvements, all of which fall within the scope of the present invention to be protected.

Claims

1. A skin care matrix having the effect of regulating skin microecology and preventing skin loss, characterized in that: The composition comprises the following components in parts by weight: Active ingredient A: 60-70 parts of filtrate of galactosyl yeast fermentation product; Active component B: 0.05-0.5 parts of Platycladus orientalis leaf extract, 0.05-0.5 parts of citrus fruit extract, 0.05-0.5 parts of white willow bark extract, 0.05-0.5 parts of magnolia bark extract, 0.05-0.5 parts of lotus leaf extract, 0.05-0.5 parts of sea buckthorn fruit extract, 0.05-0.5 parts of ceramide NS / ceramide NG, 0.05-0.1 parts of Italian helichrysum extract, 1.0-5.0 parts of lactobacillus / soybean extract fermentation product filtrate, 0.5-1.0 parts of vitex rotundifolia extract, 0.05-0.3 parts of hydrolyzed keratin, and 0.05-0.3 parts of hydrolyzed vegetable protein.

2. The skin care matrix having the effect of regulating skin microecology and preventing skin loss according to claim 1, characterized in that: The preparation method of the lotus leaf extract comprises the following steps: First, the mature lotus leaves were washed and immersed in an enzyme solution for pretreatment for 4 hours. The enzyme solution was a mixture of lipase powder and water in a weight ratio of 0.5:99.5, with a pretreatment temperature of 60°C, pH 8.0, and a stirring rate of 50 rpm. Then the lotus leaves were taken out, rinsed, drained, crushed and air-dried at room temperature until the water content was 20wt%; Finally, the lotus leaf powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the lotus leaf extract.

3. The skin care matrix having the effect of regulating skin microecology and preventing skin loss according to claim 1, characterized in that: The preparation method of the Italian immortelle extract comprises the following steps: First, the mature Italian immortelle plants were washed and immersed in an enzyme solution for pretreatment for 2 hours. The enzyme solution was a mixture of lipase powder, water and alkyl glycoside in a weight ratio of 1:98.7:0.3, with a pretreatment temperature of 60°C, a pH of 8.0 and a stirring rate of 150 rpm. Then, the Italian immortelle plants were taken out, rinsed, drained, crushed, and air-dried at room temperature until the water content reached 20 wt %; Finally, the Italian Immortelle plant powder was mixed with anhydrous ethanol in a weight ratio of 1:12, and the mixture was heated under reflux for extraction for 3 times, each time for 1.5 h. The extracts were combined and decompressed to obtain an extract. The extract was added with water to form a suspension, which was loaded onto a macroporous resin column and eluted with a 40wt% ethanol aqueous solution. The effluent was concentrated under reduced pressure and then dried to obtain the Italian Immortelle plant extract.

4. A cosmetic, characterized in that: The composition comprises the following components in parts by weight: 62-77.2 parts of the skin care matrix according to claim 1, 0.2-0.3 parts of guar hydroxypropyltrimonium chloride, 13-16 parts of sodium laureth sulfate, 1.5-3.0 parts of sodium lauroyl sarcosinate, 1.5-3.0 parts of sodium lauroyl glutamate, 1.5-2.0 parts of ammonium lauryl sulfate, 0.3-0.5 parts of sodium lauryl sulfate, cocamide methyl MEA 1.0-1.5 parts, glycerin 0.5-1 parts, allantoin 0.2-0.3 parts, citric acid 0.1-0.15 parts, dichlorobenzyl alcohol 0.1-0.11 parts, sodium isostearyl lactylate 0.05-0.1 parts, cocamidopropyl betaine 3.0-5.0 parts, menthol 0.2-0.25 parts, piroctone olamine salt 0.08-0.1 parts, flavor 0.5-0.8 parts, cocamidopropyl PG-dimethyl ammonium chloride 0.5-0.8 parts, PPG-3 caprylyl ether 0.5-1.0 parts, phenoxyethanol 0.4-0.5 parts, sodium benzoate 0.2-0.4 parts, cocodimonium hydroxypropyl hydrolyzed wheat protein 0.2-0.3 parts and polyester-11 0.11-0.15 parts.

5. A method for preparing cosmetics according to claim 4, characterized in that: The following steps are involved: Step 1, weighing the formula components, mixing the active component A in the skin care matrix with guar gum hydroxypropyltrimethylammonium chloride to obtain a first group of materials, mixing sodium laureth sulfate, sodium lauroyl sarcosinate, sodium lauroyl glutamate, ammonium lauryl sulfate, sodium lauryl sulfate, cocamide methyl MEA, glycerin, allantoin, citric acid, dichlorobenzyl alcohol and sodium isostearyl lactylate to obtain a second group of materials, mixing cocamidopropyl betaine, menthol and piroctone olamine salt to obtain a third group of materials, and mixing the active component B in the skin care matrix with essence, cocamidopropyl PG-dimethyl ammonium chloride, PPG-3 octyl ether, phenoxyethanol, sodium benzoate, cocodimonium hydroxypropyl hydrolyzed wheat protein and polyester-11 to obtain a fourth group of materials; Step 2, adding the first group of materials during stirring, and then homogenizing to obtain a first slurry; Step 3, stirring the first slurry and heating it, adding the second group of materials during stirring, and obtaining the second slurry after keeping the temperature; Step 4, cooling the second slurry, adding the third group of materials during cooling, and stirring to obtain a third slurry; Step 5, cooling the third slurry to 45° C., adding the fourth group of materials, and stirring to obtain a fourth slurry; Step 6, stirring the fourth slurry for the first time and cooling it to 30-40° C., and then stirring it for the second time before discharging.

6. The preparation method according to claim 5, characterized in that: In the step 2, the stirring temperature is 25-35° C., the stirring frequency is 60-80 Hz, and the stirring time is 5 min; the homogenizing speed is 1800-2000 rpm, and the homogenizing time is 3-5 min.

7. The preparation method according to claim 5, characterized in that: In step 3, the stirring frequency is 200-300 Hz, the temperature is raised to 80-85° C., and the heat preservation time is 30 minutes.

8. The preparation method according to claim 5, characterized in that: In step 4, the stirring frequency is 60-80 Hz and the stirring time is 10-15 min.

9. The preparation method according to claim 5, characterized in that: In step 5, the stirring frequency is 60-80 Hz and the stirring time is 10-15 min.

10. The preparation method according to claim 6, characterized in that: In step 6, the first stirring speed is 30-60 rpm, and the temperature is lowered to 30-40° C.; the second stirring speed is 30-60 rpm, and the stirring time is 5-30 min.

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