773 results about "Superoxide dismutases" patented technology

The invention provides a method for treating a medical condition, disease, or disorder mediated by a misfolded form of superoxide dismutase (SOD) in a subject in need of treatment. The method optionally comprises administering to the subject a composition comprising a pharmaceutically acceptable vehicle and an agent selected from (1) an exogenous antibody or fragment thereof that binds selectively to the misfolded form of SOD, and / or (2) an immunogen that elicits production of an endogenous antibody that binds selectively to the misfolded form of SOD, and / or (3) a nucleic acid sequence encoding (1) or (2). In certain embodiments, the invention provides methods of treating diseases such as Alzheimer's Disease, Parkinson's Disease or amyotrophic lateral sclerosis using amyotrophic disease-specific epitopes, and compositions including these epitopes. The invention also provides antibodies that bind to monomeric or misfolded SOD1, and not on the molecular surface of native homodimeric SOD1. In addition, the invention includes methods of diagnosing Alzheimer's Disease, Parkinson's Disease or amyotrophic lateral sclerosis in a subject. Also, the invention provides methods of identifying substances for the treatment or prevention of Alzheimer's Disease, Parkinson's Disease or amyotrophic lateral sclerosis and kits using the binding proteins of the invention.

The invention is concerned with a kind of Rosa roxburghii Tratt candy produce. It produces the syrup of pure Rosa roxburghii Tratt or compound Rosa roxburghii Tratt into horniness candy, horniness candy with filling, soft candy, milk lipid candy, polishing candy, glue-based candy, charging candy, chocolate candy or jelly, and so on. It increases the stability of vitamin C, vitamin P and superoxide dismutase and affords a kind of high quality candy food with double functions of nutritional health care and relaxing. The produce method relates to prepare clarified Rosa roxburghii Tratt concentrated syrup with good stability, add assistant stuff as the amount of 1 to 50 percent of whole amount into the said syrup, prepare Rosa roxburghii Tratt grain or Rosa roxburghii Tratt fast-dissolving powder and follow the routine technology to produce horniness candy, horniness candy with filling, gel candy, glue-based candy, charging candy, chocolate candy or jelly, and so on.

The present invention relates to multifunctional steroid compounds combining a steroid component with SOD mimic component and optionally also with NO donor component, and to their use in treating and preventing disorders associated with oxidative stress and free radical injury, or disorders in which treatment with steroids is indicated, whereas such combination increases the efficacy of treatment and reduces side effects associated with steroid treatment. The invention further relates to methods and devices for administering the compounds.

The technology is a veterinary pharmaceutical formulation of two vaccines, one from an RNA viral vector system constituted by an RNA recombinant particle that codifies for a Cu / Zn superoxide dismutase protein of Brucella abortus, and the other based on naked RNA constituted by a recombinant molecule of naked RNA that carries a sequence for the synthesis of at least one recombinant Cu / Zn superoxide dismutase protein of Brucella abortus and some Semliki Forest virus genes. An expression system based on the Semliki Forest virus and a use of this system, in addition to a method for the preparation of the pharmaceutical formulations.

Single amino acid compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The single amino acid compounds may be used in compositions and methods to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals.

The invention discloses a preparation method of a mushroom ferment beverage with effect of improving immunity, and belongs to the technical field of food beverages. According to the method disclosed by the invention, mushrooms and / or medicinal plants, fruit and ginseng are adopted as raw materials. The method comprises the following steps: slicing, chopping or crushing the raw materials; uniformly mixing the sliced, chopped or crushed raw materials so as to obtain a mixture; after adding sugar in the mixture, fermenting the mixture in ferment microorganisms; adding sugar when the sugar content is lowered; continuing fermentation so as to obtain the mushroom ferment beverage. According to the method disclosed by the invention, ferment bacteria can regulate intestinal flora and generate SOD (superoxide dismutase), so that the raw materials can be successfully fermented; the prepared ferment beverage contains protease, lipase, SOD, pectinase, lucid ganoderma, cauliflower fungus, pleurotus eryngii polysaccharide, rare ginsenoside, Rb2, C-K and the like. According to the method disclosed by the invention, the fermentation liquor and mycelium of the mushrooms can be directly used for fermentation; compared with sporophore, the raw materials are easier to obtain, and the method has the advantages of being short in fermentation cycle, easy in formula, simple in fermentation process, and the like.

I have discovered that trace metals such as copper, zinc, iron, and manganese that are necessary for the proper functioning of superoxide dismutase (SOD) and other deactivators of active-oxygen molecules (which cause aging of skin and other skin disorders), can be delivered from the topical compositions. This is achieved by the preparation of copper and other trace metal complexes with phosphorylated nucleosides, such as nucleotides, and phosphorylated monosaccharides, such as phosphorylated glycosides which act as small molecular weight (SMW) transporter molecules. These trace metal complexes of nucleotides and glycosides can be prepared by an in-situ method in water, water-miscible organic solvent, or a mixture of water and water-miscible organic solvent from commonly available ingredients in concentrations that are desirable and can be accurately controlled. I have additionally discovered compositions to achieve the transport of copper from the surface layers of skin into the deeper layers of skin utilizing SMW transporter molecules; and the intra-cellular storage of copper ions in the cell, for example in a bound form with glutathione; and the intra-cellular transport of copper from glutathione to SOD apoprotein by metallochaperones; and the supply of energetic molecules, such as ATP, ADP, or phosphorylated saccharides for SOD metallochaperones to perform their intra-cellular metal transfer function. These cosmetic or pharmaceutical compositions are useful for antiaging and antiviral benefits.

The invention relates to blood lipid-lowering active fractions of forsythia suspensa and a preparation method and use of the same. The active fractions are ethyl acetate fractions obtained by concentrating an extract which is extracted from forsythia suspensa leaves by acetone and water. The compound of phillygenin prepared by the invention and the active fractions, namely the ethyl acetate fractions, at which the phillygenin is positioned have good antioxidant activity in vitro. In-vivo experiments show that the ethyl acetate fractions and the phillygenin can lower total cholesterols and low-density lipoprotein cholesterin in the blood serum of a mouse with hyperlipidemia and can lower blood lipid level by an oxidative approach (raising superoxide dismutase SOD and lowering malonydialdehyde MDA). In addition, the ethyl acetate fractions have a better effect of reducing the total cholesterols in the blood serum of the mouse than the phillygenin and cassia seed blood lipid-lowering tablets. The ethyl acetate fractions of the forsythia suspensa leaves can be further developed to play a role in the multi- target co-prevention and treatment of hyperlipidemia and other diseases and have a good application prospect.

The invention discloses river crab feed, which comprises fish meal, vegetable dregs, corn, soya bean meal, inferior meal, soya-bean oil, shrimps, cuttlefish ointment, monocalcium phosphate, mineral substances for crabs, vitamin for the crabs, a binder, 0.7 to 14.0 mg / kg of cordycepin, 131.0 to 2,620.0 mg / kg of cordycepic acid, 0.1 to 2.0 mg / kg of adenosine and 290.0 to 5,800.0 IU / kg of superoxide dismutase. The river crab feed has the high utilization rate, reduces the pollution of feed residues to environment, avoids the negative effects of antibiotic residues and tolerance caused by the antibiotic residues, is safe and harmless to the craps and the environments and can enhance the immunity of the crabs and accelerate the growth of the crabs. The invention also provides a production process of the river crab feed, which has the characteristics of low cost and simple and convenient operation.

The invention discloses a platinum nano-material with the activity of four mimic enzymes and a preparation method thereof. The preparation method comprises the following steps: using citrate ions as a modifier, reducing chloroplatinic acid by utilizing sodium borohydride, and preparing the platinum nano-material modified by citric acid. The platinum nano-material is simply and quickly prepared, the particle size is small, and the dispersibility and stability of a water solution are high. The obtained platinum nano-material has the activity of four mimic enzymes at the same time, and comprises peroxidase, oxidase, catalase and superoxide dismutase. The activity of the four mimic enzymes of the platinum nano-material can be adjusted and controlled through the concentration of the citrate ions used in the preparation process.

The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla astrisanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O2-. scavenging activity remains same before and after autoclaving; scavenges O2-. from sub-zero temperature of -20° C. to high temperature of +80° C.; O2-. scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2-. scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and / or macro-organism after autoclaving; possesses temperature optima at 0° C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53x104% per nmol per min at 0° C.; and requires Cu / Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

There is provided use of a compound in the manufacture of a medicament to inhibit superoxide dismutase (SOD) or for use in the therapy of a condition or disease associated with SOD wherein the compound is of Formula I wherein: X is a ring having at least 4 atoms in the ring; K is a hydrocarbyl group; R<1 >is a halogen or a group of the formula -L<1>-Z-R<1>, wherein L<1 >is an optional linker group, Z is O or S and R<1 >is a hydrocarbyl group or H; with the proviso that the compound is other than 2-methoxy-17beta-oestradiol, 2-methoxyoestrone and 2-hydroxyestradiol.

The invention provides topical compositions and methods for using the compositions. The compositions can be used for the treatment of fibrotic or connective tissue disorders involving scarring, sub-dermal plaque accumulations, or fibrosis of muscle tissue. The disorders can be painlessly treated by the topical application of a composition described herein. One or more calcium channel blocker agents can serve as an active ingredient of the compositions, optionally in combination with, for example, one or more of emu oil and superoxide dismutase. The composition can further include pharmaceutically acceptable carriers that can facilitate the non-invasive transdermal delivery of the active(s) to subdermal sites.

The invention discloses a blueberry SOD (Superoxide Dismutase) red wine and a preparation method thereof. The blueberry SOD red wine comprises 75ml of blueberry primary extraction solution, 100,000-200,000 units of blueberry SOD crude enzyme and 675ml of red wine. The preparation method of the blueberry SOD red wine comprises the following steps of: (1) cleaning blueberry; (2) draining water on the surface of the blueberry; (3) crushing and pulping by using a crushing and pulping machine, adding one time of volume of 4DEG C de-ionized water and carrying out ultrasound crushing at the constant temperature for 2 hours; (4) centrifuging to obtain supernate as the primary extraction solution; (5) intercepting the primary extraction solution to obtain a concentrated solution with the molecular weight of 20,000-60,000; (6) adding fucose as an SOD protecting agent and carrying out vacuum freezing and drying to obtain the blueberry SOD crude enzyme; and (7) mixing the blueberry SOD crude enzyme with the primary extraction solution, then adding the mixture into the red wine, sterilizing and bottling. The blueberry SOD wine obtained by the preparation method disclosed by the invention has the advantages of abundant nutrient components, favorable mouth feeling and high SOD survival rate.

The invention provides a SOD gene from animal and a method for cloning and expressing the SOD gene in colibacillus and yeast cells, wherein a nucleotide sequence of superoxide dismutase is shown as SEQ NO.1; an amino acid sequence of the superoxide dismutase is shown as SEQ NO.2; carriers of nucleotide molecules are colibacillus plasmids or yeast plasmids; cells of the nucleotide molecules are formed by carrier conversion; and cells of the nucleotide molecules of the superoxide dismutase contain colibacillus containing the nucleotide molecules or converted by the carriers or pichia yeast containing the nucleotide molecules or converted by the carriers. The invention can prepare recombinant production strains which can efficiently express and secrete Cu / Zn-SOD, realizes the production industrialization of the Cu / Zn-SOD, and achieves good pH stability, favorable thermal stability and Cu / Zn-SOD products with anti-protease hydrolyzation capacity.

The invention belongs to the technical field of biological monitoring environment, and specifically relates to a shellfish monitoring method for ocean oil spill pollution. The shellfish monitoring method comprises the steps of utilizing typical bivalve mollusk such as short-necked clam and chlamys farreri in China to determine the shellfish visceral mass autioxidant enzyme activities including superoxide dismutase, glutathione glutathione S-transferase, peroxidase, glutathione peroxidase and catalase activity, integrating the enzyme activities so as to form an integration biomarker responding index (IBR), establishing a dose-effect relationship between the IBNNR and petroleum concentration, adopting a one-dimensional variance components method (ANOVA) to carry out notable difference analysis, determining the forcing degree of petroleum pollution on organisms, and finally judging pollution level of the ocean oil spill.

The invention belongs to the field of environmental toxicology, and relates to a method for testing typical antibiotics wastewater toxicity by using zebra fishes. According to the method, zebra fishes are used as to-be-tested organisms, and are exposed in typical antibiotics wastewater, the short-term or long-term toxic effect of the typical antibiotics wastewater to the zebra fishes is quantitatively tested through analyzing the acute toxicity and subacute toxicity of the typical antibiotics wastewater to the zebra fishes, the acute toxicity is described by using the 96h medium lethal concentration (96h LC50) (typical antibiotics wastewater percent by volume percent), and the subacute toxicity is described by fish body biochemical indexes such as superoxide dismutase (SOD) activity, peroxidase (POD) activity and malondialdehyde (MDA) content. By adopting the method, the toxicity characteristics and toxicity levels of the typical antibiotics wastewater can be analyzed, tested and quantitatively described, and the problem that conventional physicochemical indexes can not reflect biotoxicity of the typical antibiotics wastewater can be solved, and results obtained by analyzing, testing and describing can be used as biotoxicity monitoring and evaluating indexes of the typical antibiotics wastewater.

The invention achieves control over mixing controlled atmosphere storage of fruit vegetables by preparing an air-conditioned cold store, selecting fruits and vegetables, precooling, and stacking. Fruits and vegetables, upon pickup. are precooled to 9-11° C. in a vacuum pre-cooler for 12-24 hours. They are then stacked in a controlled atmosphere storage house. O2, CO2, N2 and Ar with different proportions are used for modified atmosphere packaging. The invention can extend the storage of mixing storage of various kinds of typical fruits and vegetables to more than 50 days, during which the fruits and vegetables are maintained in good color and flavor and have higher peroxidase, superoxide dismutase, catalase and cellulose activity; also, accumulation of hazardous substances such as superoxide anion free radical, hydrogen peroxide and malonaldehyde is effectively reduced, the retention rate of Vc is up to 80%-90%, and the weight loss ratio can be controlled to be below 8%.

The invention relates to a Chinese wolfberry enzyme and a preparation method thereof. For the first time, traditional medicinal and edible Chinese wolfberries are used as raw materials for preparing the enzyme, according to preparation conditions, natural generation conditions of the enzyme are stimulated, the principle is scientific, equipment is simple, and the Chinese wolfberry enzyme is suitable for mass production. Enzyme products obtained through fermentation not only have rich proteases, lipases, amylases, cellulases, superoxide dismutase, metabolism products and a large number of probiotic cells, but also have the efficacies of nourishing bodies and health care of Chinese wolfberries; the enzyme products not only fill in the blank of the prior art but also have high nutrient value and good health care efficacies; after being eaten for a long term, the enzyme products can have the medical value and the health-care effects of expelling toxins, nourishing faces, softening blood vessels, improving microcirculation, improving the antioxidant ability of organisms, delaying ageing, alleviating asthenopia and the like.

A stabilized superoxide dismutase (SOD1) analogue, wherein the side chains of two amino acids on two different SOD1 monomers are connected is provided. A method of producing a stabilized superoxide disumutase (SOD1) analogue comprises reacting a first SOD1 monomer, a second SOD1 monomer, and a cross-linker.

The invention discloses a method detecting activity of plant superoxide dismutase, catalase and peroxidase, which comprises the steps of grinding a sample by using a liquid nitrogen method, extracting three enzymes simultaneously by adopting one buffer solution, detecting enzyme activity and the like. In the method, SOD, CAT and POD in plants can be simultaneously extracted by adopting one buffer solution, so that the method fulfills the aim of simply and quickly detecting the enzyme activity of the SOD, CAT and POD, simplifies operation, saves time and labor, reduces the using amount of samples and reagent and the like, and contributes to the realization of batch operation, and the detection result is similar to that of the conventional method. Furthermore, the sample is ground by adopting a liquid nitrogen grinding method before enzyme solution is extracted, so that the method has the advantages of high grinding speed, good cell crushing effect and high extraction efficiency.

The invention discloses a bacillus coagulans strain capable of resisting streptococcus and applications of the bacillus coagulans strain. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.12196. The strain is prepared into fungus powder which is added to an animal feed, the fungus powder also can beapplied as a microbial preparation for resisting streptococcus, and after the feed containing the strain is used for feeding tilapia mossambica, streptococcicosis can be effectively prevented and treated. The strain is mixed with bacillus subtilis and lactobacillus johnsonii, then a compound microbial preparation is prepared, and the compound microbial preparation can improve the growth performance of tilapia mossambica, and improve the activity of superoxide dismutase and catalase of the liver and lysozyme in serum. In addition, the fermentation broth of the strain can be adopted as a breeding environment modifier, after the fermentation liquor of the strain is sprayed to an aquaculture pond of tilapia mossambica, the quantity of streptococcus in the aquaculture water can be reduced, andnitrite in the water can be eliminated under the low oxygen condition.

The invention discloses a cultivation method for a fruit tree bearing fruit rich in superoxide dismutase. The cultivation method is a planting method which employs one or more microbial preparations to produce fruit rich in copper-zinc superoxide dismutase (Cu / Zn-SOD). Compared with the prior art, the cultivation method provided by the invention has the advantage that the content of SOD is greatly increased. By eating the fruit, the health conditions of people can be greatly improved; elimination of sub-health can be facilitated; immunity can be enhanced; excess superoxide anion free radicals in human bodies can be scavenged; and occurrence of a plurality of diseases can be prevented.

The invention relates to a health preparation rich in lutein and Beta-carotene, which is fruit pulp or fruit powder prepared with 50% to 90% of carrot and 10% to 50% of fresh petals of marigold in weight ratio, or capsule or tablets prepared further with the fruit powder. The health preparation is characterized in that, considering the preparation method, as the Beta-carotene in carrot and the lutein in marigold are fat-soluble vitamin and significantly different from the plant cytoderm in mass, both the Beta-carotene and the lutein are mixed and extracted centrifugally after cell disruption, thereby, the lutein and the Beta-carotene are enriched in the extracted fruit pulp economically and effectively, and allow to be directly used for development and utilization of functional food. The health preparation has the advantages that the combined medicinal function of natural plants of marigold and carrot is fully functioned; experiments prove the combination of Beta-carotene and the lutein enables to enhance the activity of superoxide dismutase in blood, compared to taking fruit pulp of Beta-carotene or fruit pulp of lutein individually, the health prevention effect is improved obviously.

The invention relates to a preparation method for synthetizing a NaA type molecular sieve membrane in seed crystal aerosol. An out-of-phase SOD (Superoxide Dismutase) type molecular sieve seed crystal is adopted as induction to synthetize a nano NaA type molecular sieve crystal in fluorine-containing aerosol, a support modified by a silane coupling imidazolium cation based ionic liquid ethanol solution is put in synthesis aerosol containing the nano NaA type molecular sieve crystal, and a compact and continuous NaA type molecular sieve crystal layer is formed on the surface of the modified support by adopting a hydrothermal synthesis method. The surface of the prepared NaA type molecular sieve crystal membrane is continuous and compact, the separation performance is good and the repeatability is good, and the method is suitable for being used in large-scale preparation of the NaA type molecular sieve crystal membrane and application of industrial solvent dehydration of the NaA type molecular sieve membrane.

The invention provides a coproduction process for extracting various bioactivators from pig placenta, which comprises the following steps: taking the pig placenta (containing farrowing blood) as a raw material, utilizing components rich in the raw material such as immune globulin (Ig), immunocompetent peptide, superoxide dismutase (SOD), lipopolysaccharide, placental protein and the like, and applying modern biochemistry technology and biological integration technology such as salting in, salting out, chromatography, ultra-filtration and the like according to different structures, molecular weights, isoelectric points, physical and chemical properties, biological activities of the components to make the coproduction process suitable for industrial production. Therefore, in the same flow of the production process, the effective components in the pig placenta with market development value can be produced, namely five products of the immune globulin, the immunocompetent peptide, the superoxide dismutase, the lipopolysaccharide and the placental protein. The process combines the conventional and modern processes, produces various products through coproduction, and not only makes full use of placenta resources to change the waste into the treasure, does not discharge waste liquid and prevents the environment, but also can greatly reduce the production cost and improve the economic benefit.

The invention discloses a hydrolat moisturizing facial mask. The method comprises the following steps: putting a hydrolat with moisturizing efficacy into a container, then adding hyaluronic acid, fish collagen, water soluble vitamin and lauric acid modified superoxide dismutase into the container, and stirring the solution until the solution is completely dissolved; then adding aloe extracting solution and supguard GM-BP preservative into the solution, and uniformly stirring, uniformly dripping the obtained liquid onto a non-woven fabric facial mask, then conducting sealed packaging to obtain a product, namely the hydrolat moisturizing facial mask. The hydrolat with moisturizing efficacy includes rose hydrolat, witch hazel hydrolat, jasmine hydrolat, marigold hydrolat, sandalwood hydrolat and the like, and any one of the hydrolat or combination of any two or more of the hydrolat can be adopted. The hydrolat moisturizing facial mask prepared by adopting the method disclosed by the invention has the advantages that the moisturizing effect is prominent, and the mask is safe and can not irritate a human body, and the like.