429 results about "Superoxide dismutases" patented technology

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a tumor cholesterol metabolism regulation microneedle patch and a preparation method, the microneedle patch comprises a needle tip and a backing which are connected, the needle tip comprises a needle tip body and nanoparticles loaded on the needle tip body, the nanoparticle is a manganese ion-doped organic metal framework, the outer layer of the manganese ion-doped organic metal framework is modified with a targeting agent, cholesterol oxidase and superoxide dismutase are carried on the manganese ion-doped organic metal framework, the targeting agent can target a CD44 receptor, and the organic metal framework can carry drugs. The targeted drug can enter tumor cells in a targeted mode, superoxide dismutase catalyzes superoxide anions overexpressed in the tumor cells to generate H2O2 and O2, O2 needed by catalysis is provided for cholesterol oxidase, cholesterol at the tumor site is consumed by the cholesterol oxidase, accumulation of 7-DHC is reduced, meanwhile H2O2 can be generated, and the cholesterol oxidase can be used for catalyzing the tumor site. H2O2 is catalyzed by manganese ions through a Fenton-like reaction to generate OH to induce tumor cells to generate ferroptosis, so that ferroptosis-immune synergistic treatment is realized.

The invention relates to ultra-small prussian blue nano-particles (T-USPB-C) carrying tirofian, a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for reducing MVO and MIRI. According to the preparation method, the T-USPB-C is prepared by three steps. The T-USPB-C provided by the invention can carry tirofiban, and has catalase and superoxide dismutase simulated nano-enzyme activity. Moreover, the size of the nano-scale drug is required to reach a nano-scale size, and the drug can be efficiently cleared through kidney excretion, so that the potential long-term toxicity problem is minimized. The ultra-small prussian blue nanoparticle carrying the tirofian has an excellent active oxygen scavenging capability. Microvascular perfusion can be rapidly improved through the antithrombotic effect, then the protection effect is continuously achieved through the anti-oxidation and anti-inflammatory mechanism, and microvascular injury and MIRI are effectively prevented.

The invention belongs to the technical field of catalytic materials, and particularly relates to high-entropy alloy nanoparticles used as a biocatalyst and application of the high-entropy alloy nanoparticles. The PtFeCuCoNi high-entropy alloy nano-particles with pH adaptability are developed through a one-step solvothermal synthesis method, it is found that the PtFeCuCoNi high-entropy alloy nano-particles can serve as potent oxidase, can generate reactive oxygen species (ROS) and effectively kill bacteria such as MRSA under the acidic condition, and can play the functions of superoxide dismutase and catalase under the neutral condition, so that the PtFeCuCoNi high-entropy alloy nano-particles with the pH adaptability can be used for effectively killing bacteria such as MRSA. Active oxygen in cells is efficiently removed, oxidative stress is relieved, and the cell protection effect is achieved.

Provided is a superoxide dismutase-based nanoscale transdermal delivery system, which is a capsule composite structure consisting of a small molecule active ingredient, superoxide dismutase, and a polymer coating. The small molecule active ingredient is loaded in the superoxide dismutase, and the surface of the superoxide dismutase is coated with the polymer coating. The superoxide dismutase-based active ingredient transdermal delivery system has a diameter of 20-100 nm, and the polymer coating has a thickness of 7.5-52.5 nm. In the delivery system, the polymer coating protects the superoxide dismutase and the small molecule functional substance having an antioxidant effect, and the capsule composite structure is in the form of nanogel. The nanogel not only promotes the penetration depth of the delivery system, but also mitigates the stimulation of the delivery system to the skin. The delivery system features biofriendly starting materials, simple preparation method, and high yield, and thus can be produced in large scale industrially.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a codon optimized superoxide dismutase AfSOD gene and application thereof in degradation of aflatoxin B1 and zearalenone. Under the conditions that the pH is 7, the temperature is 40 DEG C and the enzyme addition amount is 10 micrograms, the degradation rate of the AFB1 reaches 99.08% within 24 hours, and the degradation rate is kept to be 80% or above within 40-80 DEG C; under the conditions that the pH is 8, the temperature is 60 DEG C and the enzyme addition amount is 20 micrograms, the degradation rate of ZEN reaches 85.1% in 24 hours, and the degradation rate is kept to be 80% or above within 50-80 DEG C; the result shows that AfSOD is a toxin degrading enzyme which can tolerate a certain degree of high temperature; the degradation rates of AFB1 and ZEN in polluted peanut powder or corn powder are 73.7% and 88.2% respectively; the invention provides a new candidate for detoxification of AFB1 and ZEN in food or feed, and has a good application prospect.

The invention provides application of qi-blood foundation strengthening oral liquid in preparation of a medicine for building body and resisting aging, and belongs to the technical field of medicine preparations. According to the invention, the mass ratio relationship among the components and the preparation method thereof are researched, and especially in the preparation process, the traditional Chinese medicine components are extracted by adopting the modes of high-pressure steam heating, vacuum concentration and the like under different pressures in batches and stages, so that the obtained oral liquid for consolidating qi and blood not only has the effective component content, but also has the health-care effect. The oral liquid for consolidating qi and blood has the effects of improving the activity of superoxide dismutase of red blood cells and reducing the content of lipofuscin in tissues, can improve the exercise tolerance of rats, and has the effects of resisting aging and building the body.

The invention relates to a modified superoxide dismutase pH response type nano-liposome and a synergistic anti-photoaging after-sun repair composition capable of achieving accurate targeting. The modified superoxide dismutase pH response type nano-liposome is obtained by modifying a superoxide dismutase nano-liposome with hyaluronic acid, and the modified superoxide dismutase pH response type nano-liposome has the advantages that the modified superoxide dismutase pH response type nano-liposome can be accurately targeted by the aid of the synergistic anti-photoaging after-sun repair composition; the superoxide dismutase nano-liposome is of a core-shell structure, a core layer is superoxide dismutase, a shell layer is a ternary composite liposome, and the ternary composite liposome is formed by compounding HSPC, DSPC and cholesterol. By constructing a pH response type nano delivery system, the pH drop characteristic of an ultraviolet damage area can be accurately recognized, controlled release of SOD at a target site is triggered, O2 <-> and H2O2 are synchronously removed, an oxidative stress chain reaction is blocked, and in cooperation with the inhibition of tetrahydrocurcumin on a tyrosinase signal channel and the anti-inflammatory repair effect of the aloe extract, the anti-inflammatory repair effect of the aloe extract on the tyrosinase signal channel is achieved. A three-dimensional action network of targeted removal-activity regeneration-barrier reconstruction is formed, and the photoaging prevention and treatment effect is improved.

The present invention relates to intracellular copper zinc superoxide dismutase inhibitors for protecting a subject from damage caused by radiation outbreak. The invention also relates to kits comprising SOD1 inhibitors and radiosensitizers for cancer cells, to the in vitro use of SOD1 inhibitors for protecting non-cancer cells from ionizing radiation, and to methods, compositions and uses related thereto.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention belongs to the technical field of food and medicine, and relates to application of corn germ concentrated powder in improvement of osteoarthritis. The invention particularly relates to application of corn germ concentrated powder, namely corn antioxidant multienzyme, in preparation of medicines or health-care foods for treating rheumatoid arthritis and osteoporosis. In the corn germ concentrated powder, superoxide dismutase / (U / g) is greater than or equal to 8500, glutathione peroxidase / (U / g) is greater than or equal to 5000, and catalase / (U / g) is greater than or equal to 750. The corn germ concentrated powder can also be prepared by the following method: cleaning corn, germinating, adding water into the germinated corn, grinding and pulping, adding a settling agent into the corn steep liquor, standing, taking supernate, adding part of sodium ascorbate, performing temperature-controlled concentration, adding the rest sodium ascorbate and a stabilizer into the concentrated solution, and uniformly mixing to obtain the corn germ concentrated powder. And drying at controlled temperature, crushing, and mixing with a proper amount of silicon dioxide. The prepared corn germ concentrated powder has the effect of resisting rheumatoid arthritis.

The invention relates to the technical field of biology, and discloses an efficient extraction process of SOD in silphium perfoliatum, which comprises the following steps: mixing a silphium perfoliatum superoxide dismutase crude extract, polyethylene glycol and a first salt, and adjusting the pH value to form an aqueous two-phase system to enrich SOD; adding polysaccharide hydrolase and protease into the system to carry out in-situ enzymolysis so as to degrade macromolecular impurities; after the enzymolysis is finished, adding a second salt for competitive distribution, and selectively removing the added functional enzyme to a salt phase; and finally, separating and collecting a supernatant phase rich in superoxide dismutase. By integrating aqueous two-phase extraction, in-situ enzymolysis and competitive distribution into a single reaction system, a tedious intermediate separation unit in a traditional process is replaced, and by continuous and targeted removal of endogenous macromolecular impurities and exogenous functional enzymes, the purity and specific activity of a final product are remarkably improved, so that the method is suitable for industrial production. And the biological activity of SOD is effectively protected by the mild separation conditions in the whole process.

The invention belongs to the technical field of biological medicines, and particularly relates to an MXene nano material with high SOD (superoxide dismutase) enzyme activity as well as a preparation method and application thereof. The MXene nano material with high SOD (superoxide dismutase) enzyme activity, disclosed by the invention, comprises Mo2C MXene and a metal atom anchored on the Mo2C MXene. The MXene nano material with high SOD enzyme activity has the beneficial effects that the MXene nano material with high SOD enzyme activity has relatively high SOD enzyme activity and a strong ROS removal characteristic, and oxidative stress injury can be relieved; the compound has the effect of treating ROS related diseases such as traumatic brain injury (TBI), Parkinson's syndrome (PD) and chronic obstructive pulmonary disease.

The invention discloses an application of recombinant human copper / zinc superoxide dismutase in preparation of a medicine for treating triple negative breast cancer. The amino acid sequence of the recombinant human copper / zinc superoxide dismutase is shown as SEQ ID NO.1. The invention further discloses a preparation method of the recombinant human copper / zinc superoxide dismutase. The medicine for treating the triple negative breast cancer contains recombinant human copper / zinc superoxide dismutase. The medicine for treating the triple-negative breast cancer, which is prepared by recombining and transforming the human copper / zinc superoxide dismutase, can effectively control in-vitro migration of a triple-negative breast cancer cell line and tumor growth of a triple-negative breast cancer tumor-bearing mouse, so that the lifetime of the tumor-bearing mouse is effectively prolonged; therefore, the medicine for treating the triple-negative breast cancer has a very positive effect on treatment of the triple-negative breast cancer.

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

Complexes of polyoxometalate (POM) cluster anions and metal oxide nanocrystal (NC) cores (POM-NC) with tunable NC surface protonation are disclosed. These protonated POM-NC complexes enable precise control over catalytic properties, enhancing catalytic activity under near-neutral pH conditions while improving stability and solubility across various applications. The protonated POM- metal oxide complexes facilitate the activation of airborne O2 under ambient conditions and the production of reactive oxygen species (ROS), including hydroxyl radicals, superoxide, and peroxides. In certain catalytic processes, these complexes mimic enzymatic functions, such as oxidase, peroxidase, catalase, and superoxide dismutase activity, making them valuable for various biological, therapeutic, chemical and environmental applications such as applications related to oxidative catalysis and ROS scavenging.

The invention discloses a preparation method and application of a multifunctional layered nanosheet. The biomimetic hydrogel is prepared through a hydrothermal method, folic acid (FA) is modified on the surface of the biomimetic hydrogel, and the biomimetic hydrogel has the capacity of targeted recognition of pro-inflammatory phenotype macrophages, has the biomimetic activity of multiple enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and can effectively remove ROS; meanwhile, a succinate dehydrogenase (SDH) inhibitor dimethylmalonic acid (DMM) is loaded in an intercalation mode of the nano material, SDH activity is inhibited in a slow release mode, ROS outbreak caused by mitochondrial reverse electron transfer (RET) is blocked from the source, then a mitochondrial autophagy pathway mediated by BNIP3-LC3B is started, and dynamic and steady state recovery of mitochondria is achieved. The system finally realizes macrophage mitochondrial function reconstruction, oxidation environment improvement and transfer of healthy mitochondria to osteoblasts, so that the osteogenic differentiation capability is recovered, and the effect of treating osteoporosis is achieved.

The invention discloses an SOD2 knockout cell line and application of the SOD2 knockout cell line in anti-poxvirus, and relates to the technical field of antiviral research. According to the invention, sgRNA of targeted superoxide dismutase 2 (SOD2) is designed, the sequence of the sgRNA is shown as SEQ ID NO.1-SEQ ID NO.2, then a stable SOD2 knockout human-derived non-small cell lung cancer A549 cell line is established by combining CRISPR / Cas9 gene editing with a lentiviral vector delivery technology, single-cell cloning is obtained through multiple rounds of puromycin screening, and the SOD2 gene knockout A549 cell strain is successfully constructed. Vaccinia virus Tian Tan strain infection shows that the number of plaques of SOD2 knockout cells is obviously greater than that of the plaques (about 2.3 times) of normal cell infection, and the plaques are relatively large, so that fine SOD2 has the effect of limiting intercellular transmission of poxvirus. The invention lays a foundation for research and preparation of antiviral drugs.

Provided herein are compositions, systems, kits, and methods for treating a patient with a thromboembolism by administering nanoconjugates comprising nanoparticles encapsulating and / or conjugated to: i) tissue-type plasminogen activator (tPA), and ii) at least one antioxidant enzyme selected from the group consisting of: superoxide dismutase, glutathione peroxidase, glutathione reductase, and a catalase.

The invention is suitable for the technical field of pesticide residue detection, and provides a method for distinguishing and detecting pesticide based on copper-platinum bimetallic nano-enzyme. According to the method, a colorimetric sensing array with three sensing channels is constructed by synthesizing a copper-platinum bimetallic nano-enzyme (CuPtNPs) with oxidase-like activity, laccase-like activity and superoxide dismutase-like activity. A fingerprint spectrum is obtained based on specific effects of different pesticides and enzyme-like activities, and qualitative distinguishing and quantitative detection of various pesticides are realized by combining a machine algorithm. According to the method, nine pesticide residues can be measured at a time, six of the nine pesticide residues can be quantitatively analyzed, and the problem that a traditional method only can be used for single detection is solved. A strategy of constructing three channels based on a single nano-enzyme receptor CuPtNPs has the remarkable advantages of simple synthesis process, convenient operation flow and low detection cost, is good in applicability in actual agricultural product samples such as Chinese cabbages and apples, and provides an efficient solution for high-throughput detection of pesticide residues.

The invention discloses preparation of an antibacterial copper-based nano-enzyme fiber dressing capable of promoting wound healing, and belongs to the technical field of medical use. According to the method, 5, 6-dimethylbenzimidazole and copper salt are used as raw materials, the copper-based nano-enzyme is synthesized through a hot bath reaction method of a solvent, reactants are uniformly dispersed in a solution, and the defects that traditional nano-enzyme element components are not uniform, and active sites are low in density are overcome. The prepared copper-based nano enzyme has a unique metal active center, and has oxidase-like activity, catalase-like activity and superoxide dismutase-like activity. According to the copper-based nano-enzyme Cu-NPs II, the activity of oxidase can be achieved without hydrogen peroxide, active oxygen can be generated to achieve the bactericidal effect, when excessive ROS is generated, superoxide dismutase-like activity of the copper-based nano-enzyme Cu-NPs II converts the excessive ROS into H2O2, and due to the fact that the copper-based nano-enzyme Cu-NPs II has catalase-like activity and can decompose H2O2 into water and oxygen, the copper-based nano-enzyme Cu-NPs II has good microenvironment adjusting capacity.

The invention belongs to the field of new daily chemical materials, and discloses a composition with a skin improving effect, application and cosmetics, the composition comprises acetylhydroxyproline and a white water lily extract, and the invention further discloses a whitening composition. The composition comprises ergothioneine, superoxide dismutase, acetyl hydroxyproline, a white water lily extract and a malt extract. The whitening composition realizes the whitening performance through double saccharification resistance, double oxidation resistance and inhibition of melanin and tyrosinase pathways, and also has potential anti-wrinkle and firming effects at the same time.

The invention discloses an application of a pyrrosia lingua aqueous extract in preparation of a medicine for preventing and treating radioactive intestinal injury. Radioactive intestinal injury is a common complication of ionizing radiation, and has main pathological characteristics of intestinal mucosal barrier destruction, excessive release of inflammatory factors, increase of oxidative stress level and hematopoiesis and immune function inhibition. At present, no effective and safe prevention and treatment medicine is available. It is found for the first time that the pyrrosia lingua aqueous extract can inhibit expression (IL-6, TNF-alpha and IL-1beta) of inflammatory factors of radiation-induced MODE-K cells and relieve the oxidative stress level (malondialdehyde (MDA) is reduced, and the content of superoxide dismutase (SOD) and glutathione (GSH) is increased). In animal experiments, the pyrrosia lingua aqueous extract can reduce the levels of TNF-alpha and IL-1beta inflammatory factors in serum of irradiated mice and improve oxidative stress disorders such as MDA increase, SOD and GSH decrease and the like; the reduction of leukocytes and lymphocytes is relieved; the length of intestinal villus is promoted and recovered; the proportion of Ki67 positive cells in a crypt region is increased, so that the regeneration capacity of intestinal epithelial cells is enhanced. The pyrrosia lingua aqueous extract disclosed by the invention provides an experimental basis and an application prospect for developing a novel medicine for preventing and treating the radioactive intestinal injury.

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

The invention provides a nano-enzyme with sterilization, tooth desensitization and remineralization functions as well as a preparation method and application thereof, and relates to the technical field of nano-enzymes. Berberine, hemin and calcium fluoride are used as raw materials and are self-assembled into the multifunctional BHCF nano-enzyme with sterilization, tooth desensitization and remineralization functions through multi-dimensional interaction of ionic bonds, Ca < 2 + > coordination bonds, pi-pi accumulation and hydrogen bonds. The BHCF nano-enzyme has a clear peroxidase-like and superoxide dismutase-like double-enzyme catalytic capability and a photodynamic effect, the antibacterial activity of the BHCF nano-enzyme is obviously activated under an illumination condition, and the sterilization rate can reach 99.995%; meanwhile, the BHCF nano-enzyme has a good immediate and lasting sealing effect, and has excellent acid resistance, wear resistance and remineralization capacity. The BHCF nano-enzyme provided by the invention has huge application potential in preparation of drugs for treating periodontitis.

The invention relates to the technical field of oxygen free radical detection, in particular to a manufacturing process of a portable and reliable oxygen free radical detector. Comprising the following steps: mixing superoxide dismutase, potassium ferricyanide, an isothiazolinone preservative, hexammine ruthenium chloride, polyethylene glycol octylphenol ether, trehalose and bovine serum albumin, and uniformly stirring to prepare an enzyme preparation; mixing an enzyme preparation with the high polymer material solution to form reagent layer slurry with viscosity; uniformly coating the reagent layer slurry on a substrate material, and drying; printing an electrode material on the substrate material to form a working electrode and a counter electrode; the silver electrode is placed in an electrolyte containing chloride ions, potential is applied, the silver surface is subjected to a chlorination reaction, and a silver / silver chloride electrode is formed; covering the reagent layer above the electrode, tightly combining the reagent layer with the electrode in a sticking or hot pressing manner, and cutting to obtain the test paper; the prepared portable oxygen free radical detector can be used for conveniently and efficiently detecting oxygen free radicals of a human body at low cost.

The present invention relates to the field of microbial technology, and provides a Pediococcus acidilactici YYS-J2 that can degrade acrylamide and produce high levels of phenyllactic acid, and its application. Pediococcus acidilactici The preservation number of YYS-J2 is CGMCC No. 28183. This *Pediococcus lactis* YYS-J2 exhibits excellent acrylamide removal capacity, high efficiency in L-phenylalanine utilization and metabolism, high-quality phenyllactic acid production capacity, and excellent *Streptococcus mutans* antibacterial activity. It also produces superoxide dismutase (SOD) and shows good tolerance in simulated gastric and intestinal fluids. This *Pediococcus lactis* YYS-J2 can provide a new probiotic source for the development of functional products such as acrylamide removal products, antibacterial products, and SOD-producing products, and has significant application value.