558 results about "Superoxide dismutases" patented technology

The invention provides a nano transdermal delivery system based on superoxide dismutase, the nano transdermal delivery system is of a capsule composite structure of a small molecule active component-superoxide dismutase-polymer shell layer, the small molecule active component is loaded in superoxide dismutase, the surface of the superoxide dismutase is coated by the polymer shell layer, and the superoxide dismutase is loaded in the capsule composite structure. The diameter of the superoxide dismutase-based active ingredient transdermal delivery system is 20-100 nm, and the thickness of the polymer shell layer is 7.5-52.5 nm. In the delivery system, the polymer shell layer provides a protection effect for the superoxide dismutase and the micromolecular functional substances with the anti-oxidation effect, the capsule composite structure is in a nanogel form, and the nanogel not only promotes the penetration depth of the capsule, but also reduces the irritation effect of the capsule on the skin. The raw materials are biologically friendly, the preparation method is simple, the yield is high, and industrial large-scale production can be realized.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a tumor cholesterol metabolism regulation microneedle patch and a preparation method, the microneedle patch comprises a needle tip and a backing which are connected, the needle tip comprises a needle tip body and nanoparticles loaded on the needle tip body, the nanoparticle is a manganese ion-doped organic metal framework, the outer layer of the manganese ion-doped organic metal framework is modified with a targeting agent, cholesterol oxidase and superoxide dismutase are carried on the manganese ion-doped organic metal framework, the targeting agent can target a CD44 receptor, and the organic metal framework can carry drugs. The targeted drug can enter tumor cells in a targeted mode, superoxide dismutase catalyzes superoxide anions overexpressed in the tumor cells to generate H2O2 and O2, O2 needed by catalysis is provided for cholesterol oxidase, cholesterol at the tumor site is consumed by the cholesterol oxidase, accumulation of 7-DHC is reduced, meanwhile H2O2 can be generated, and the cholesterol oxidase can be used for catalyzing the tumor site. H2O2 is catalyzed by manganese ions through a Fenton-like reaction to generate OH to induce tumor cells to generate ferroptosis, so that ferroptosis-immune synergistic treatment is realized.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention relates to ultra-small prussian blue nano-particles (T-USPB-C) carrying tirofian, a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for reducing MVO and MIRI. According to the preparation method, the T-USPB-C is prepared by three steps. The T-USPB-C provided by the invention can carry tirofiban, and has catalase and superoxide dismutase simulated nano-enzyme activity. Moreover, the size of the nano-scale drug is required to reach a nano-scale size, and the drug can be efficiently cleared through kidney excretion, so that the potential long-term toxicity problem is minimized. The ultra-small prussian blue nanoparticle carrying the tirofian has an excellent active oxygen scavenging capability. Microvascular perfusion can be rapidly improved through the antithrombotic effect, then the protection effect is continuously achieved through the anti-oxidation and anti-inflammatory mechanism, and microvascular injury and MIRI are effectively prevented.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

The invention belongs to the technical field of catalytic materials, and particularly relates to high-entropy alloy nanoparticles used as a biocatalyst and application of the high-entropy alloy nanoparticles. The PtFeCuCoNi high-entropy alloy nano-particles with pH adaptability are developed through a one-step solvothermal synthesis method, it is found that the PtFeCuCoNi high-entropy alloy nano-particles can serve as potent oxidase, can generate reactive oxygen species (ROS) and effectively kill bacteria such as MRSA under the acidic condition, and can play the functions of superoxide dismutase and catalase under the neutral condition, so that the PtFeCuCoNi high-entropy alloy nano-particles with the pH adaptability can be used for effectively killing bacteria such as MRSA. Active oxygen in cells is efficiently removed, oxidative stress is relieved, and the cell protection effect is achieved.

The invention relates to the technical field of computers, in particular to a parameter optimization method and system for processing a skin abnormal area through SOD, according to the method, a reference skin area image can be flexibly selected according to an actual application scene, an SOD basic parameter vector used for assisting SOD component parameter adjustment can be determined through an image analysis and data processing process, and the SOD basic parameter vector can be used for assisting SOD component parameter adjustment. The SOD basic parameter vector does not need to be obtained through an experiment mode, the application scene of the SOD basic parameter vector can be flexibly adjusted by replacing a plurality of processed reference skin area images, so that the SOD component parameter optimization can be carried out by taking the SOD basic parameter vector as a reference, and the SOD component parameter optimization efficiency is improved. Compared with the prior art that experiments are carried out by traversing the SOD component parameters, the method has the advantages that the number of experiments in the SOD component research process can be reduced, so that the efficiency of optimizing the SOD component parameters is improved, corresponding SOD basic parameter vectors can be provided for different scenes, and the efficiency of optimizing the SOD component parameters is further improved.

The invention relates to the technical field of biological medicine, and discloses a culture medium for human metapneumovirus and application, the culture medium comprises the following components by mass: 850-920 parts of a basic culture medium; the metabolism reprogramming module is prepared from the following components in parts by weight: 0.05 to 0.15 part of 5-methyltetrahydrofolic acid, 0.3 to 0.7 part of N-acetyl-D-mannosamine and 1.0 to 3.0 parts of sodium dichloroacetate; the immune regulation and control module is prepared from 0.005 to 0.015 part of an RIG-I / MAVS pathway inhibitor; the receptor induction module is prepared from 0.5 to 1.5 parts of all-trans retinoic acid; the redox balance module is prepared from the following components in parts by weight: 0.1 to 0.5 part of pegylated superoxide dismutase and 0.002 to 0.008 part of sodium selenite. According to the method, the proportion of infectious virus particles can be remarkably increased, the generation of defective particles is reduced, meanwhile, the production cycle of host cells is prolonged, large-scale continuous culture is supported, and a high-quality and high-efficiency solution is provided for industrial production of gene therapy vectors and virus vaccines.

Provided is a superoxide dismutase-based nanoscale transdermal delivery system, which is a capsule composite structure consisting of a small molecule active ingredient, superoxide dismutase, and a polymer coating. The small molecule active ingredient is loaded in the superoxide dismutase, and the surface of the superoxide dismutase is coated with the polymer coating. The superoxide dismutase-based active ingredient transdermal delivery system has a diameter of 20-100 nm, and the polymer coating has a thickness of 7.5-52.5 nm. In the delivery system, the polymer coating protects the superoxide dismutase and the small molecule functional substance having an antioxidant effect, and the capsule composite structure is in the form of nanogel. The nanogel not only promotes the penetration depth of the delivery system, but also mitigates the stimulation of the delivery system to the skin. The delivery system features biofriendly starting materials, simple preparation method, and high yield, and thus can be produced in large scale industrially.

The invention provides a culture medium and a culture method for culturing human primary B lymphoma cells. The culture medium for culturing the human primary B lymphoma cells, disclosed by the invention, is prepared from an anti-immune globulin M antibody, a recombinant human CD40 ligand, CpG oligodeoxynucleotide 2006, superoxide dismutase, interleukin-2, interleukin-21 and interleukin-4. By using the culture medium and the culture method disclosed by the invention, effective and rapid amplification of the B lymphoma cells can be realized, the B lymphoma cells obtained by amplification maintain pathological characteristics of patients, the culture success rate and the amplification rate of the B lymphoma cells are improved, and a research basis can be provided for personalized treatment of the patients. The invention also provides B lymphoma cells obtained by in-vitro culture of the culture medium, and a method and application of the B lymphoma cells in curative effect evaluation and screening of drugs.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a codon optimized superoxide dismutase AfSOD gene and application thereof in degradation of aflatoxin B1 and zearalenone. Under the conditions that the pH is 7, the temperature is 40 DEG C and the enzyme addition amount is 10 micrograms, the degradation rate of the AFB1 reaches 99.08% within 24 hours, and the degradation rate is kept to be 80% or above within 40-80 DEG C; under the conditions that the pH is 8, the temperature is 60 DEG C and the enzyme addition amount is 20 micrograms, the degradation rate of ZEN reaches 85.1% in 24 hours, and the degradation rate is kept to be 80% or above within 50-80 DEG C; the result shows that AfSOD is a toxin degrading enzyme which can tolerate a certain degree of high temperature; the degradation rates of AFB1 and ZEN in polluted peanut powder or corn powder are 73.7% and 88.2% respectively; the invention provides a new candidate for detoxification of AFB1 and ZEN in food or feed, and has a good application prospect.

Described herein are methods of inhibiting an allele that contains a mutation in a cell (e.g., treating a subject with an allele that contains a mutation). The method includes delivering to the cell an antisense oligonucleotide that targets a variant allele that is in cis with the mutation (e.g., the mutation is a gain of function mutation or a dominant negative mutation), wherein the cell or subject is heterozygous for the target variant, and the antisense molecule is effective at knocking down expression the target variant allele (e.g., effective at preventing, treating, or ameliorating at least one symptom associated with the mutation). Also described herein are methods of haplotyping a cell or subject, and antisense oligonucleotides that target mutant superoxide dismutase 1 (SOD1) and other genes.

The invention relates to the technical field of biological medicine. The invention aims to provide an application of Mn3O4 nanoparticles in relieving intervertebral disc degeneration. The manganous-manganic oxide nanoparticles Mn3O4NPs are added into an interventional drug for treating intervertebral disc degeneration as a nano-enzyme. According to the invention, Mn3O4 nanoparticles are used as a nano active catalytic enzyme, have multienzyme catalytic activity similar to superoxide dismutase, catalase and glutathione peroxidase, can effectively remove reactive oxygen species (ROS) and regulate ECM metabolism, significantly reduce cell apoptosis, delay cell senescence, inhibit inflammation and enhance autophagy, and can be used for preparing a medicine for treating hyperplasia. Therefore, annulus fibrosus AF repair in intervertebral disc degeneration treatment is promoted. The Mn3O4 nano particles have good biocompatibility, metabolites of the Mn3O4 nano particles can be used for other physiological processes, and no toxic or side effect is generated in in-vivo circulation. Meanwhile, the enzyme is stable in low-oxygen and low-pH adverse microenvironment of the intervertebral disc, and has in-vivo stability which is not possessed by other active catalytic enzymes.

The invention provides application of qi-blood foundation strengthening oral liquid in preparation of a medicine for building body and resisting aging, and belongs to the technical field of medicine preparations. According to the invention, the mass ratio relationship among the components and the preparation method thereof are researched, and especially in the preparation process, the traditional Chinese medicine components are extracted by adopting the modes of high-pressure steam heating, vacuum concentration and the like under different pressures in batches and stages, so that the obtained oral liquid for consolidating qi and blood not only has the effective component content, but also has the health-care effect. The oral liquid for consolidating qi and blood has the effects of improving the activity of superoxide dismutase of red blood cells and reducing the content of lipofuscin in tissues, can improve the exercise tolerance of rats, and has the effects of resisting aging and building the body.

The invention belongs to the technical field of molecular biology, and particularly relates to application of an OsSCL25 gene in regulating and controlling salt stress resistance of rice. Under the stress of salt, the survival rate, chlorophyll, fresh weight and dry weight of strain seedlings of the overexpressed OsSCL25 gene are higher than those of a wild type, the content of superoxide dismutase, peroxidase and malondialdehyde is lower than that of the wild type, and the content of proline is remarkably higher than that of the wild type and a mutant. The soluble sugar content of the wild type, the soluble sugar content of the OsSCL25 mutant and the soluble sugar content of the overexpression system are not obviously different under normal treatment, and the soluble sugar content of the overexpression system is obviously higher than that of the wild type and the soluble sugar content of the gene mutant after salt stress, so that the OsSCL25 gene participates in rice salt tolerance regulation. After the gene is over-expressed, the salt tolerance of the rice is obviously improved, so that a rice material suitable for saline-alkali soil planting improvement can be cultivated by utilizing the OsSCL25 through a biotechnology, and a new thought is provided for improving the planting rate of the rice in the saline-alkali soil.

The invention relates to a modified superoxide dismutase pH response type nano-liposome and a synergistic anti-photoaging after-sun repair composition capable of achieving accurate targeting. The modified superoxide dismutase pH response type nano-liposome is obtained by modifying a superoxide dismutase nano-liposome with hyaluronic acid, and the modified superoxide dismutase pH response type nano-liposome has the advantages that the modified superoxide dismutase pH response type nano-liposome can be accurately targeted by the aid of the synergistic anti-photoaging after-sun repair composition; the superoxide dismutase nano-liposome is of a core-shell structure, a core layer is superoxide dismutase, a shell layer is a ternary composite liposome, and the ternary composite liposome is formed by compounding HSPC, DSPC and cholesterol. By constructing a pH response type nano delivery system, the pH drop characteristic of an ultraviolet damage area can be accurately recognized, controlled release of SOD at a target site is triggered, O2 <-> and H2O2 are synchronously removed, an oxidative stress chain reaction is blocked, and in cooperation with the inhibition of tetrahydrocurcumin on a tyrosinase signal channel and the anti-inflammatory repair effect of the aloe extract, the anti-inflammatory repair effect of the aloe extract on the tyrosinase signal channel is achieved. A three-dimensional action network of targeted removal-activity regeneration-barrier reconstruction is formed, and the photoaging prevention and treatment effect is improved.

The present invention discloses a preparation method of CFS nanozyme. This nanozyme has broad-spectrum bactericidal property, has low toxicity to normal cells, and there is no problem of bacterial drug resistance, and is expected to be used in the field of antibiotic-free sterilization. The CFS-ion-MN wound repair microneedle patch provided by the present invention, as a wound dressing, can transdermally deliver the antibacterial CFS nanozyme to the deep part of the wound, effectively kill bacteria. The CFS nanozyme can also inhibit inflammation in the body through its superoxide dismutase-like activity, promote wound healing, and promote the antibacterial and healing of the wounds of mice infected with Staphylococcus aureus. Among them, Co 2+ and Fe 3+ can inhibit the IL-17 and NF-κB signaling pathways, thereby accelerating wound closure.

The present invention discloses a buffer for tissue separation and / or preservation and its application, and a method for culturing tumor organoids, and relates to the field of organoid culture. The buffer is used to preserve tumor tissue samples, which are of liquid origin; the buffer includes: a basal culture medium and specific additive factors, and the specific additive factors include: superoxide dismutase, catalase, penicillin, and streptomycin; based on the volume of the basal culture medium, the concentration of superoxide dismutase is 1 to 100 μg / mL, the concentration of catalase is 50 to 500 μg / mL, the concentration of penicillin is 50 to 200 U / mL, and the concentration of streptomycin is 50 to 200 μg / mL. The tissue preservation solution of the present invention is used for the transportation and protection of liquid-derived tumor tissue samples, and has a high tumor cell activity retention rate. It is used to construct tumor organoids with a high success rate and a large number, which can meet the needs of clinical detection and disease research.

The present invention relates to intracellular copper zinc superoxide dismutase inhibitors for protecting a subject from damage caused by radiation outbreak. The invention also relates to kits comprising SOD1 inhibitors and radiosensitizers for cancer cells, to the in vitro use of SOD1 inhibitors for protecting non-cancer cells from ionizing radiation, and to methods, compositions and uses related thereto.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

The invention belongs to the technical field of food and medicine, and relates to application of corn germ concentrated powder in improvement of osteoarthritis. The invention particularly relates to application of corn germ concentrated powder, namely corn antioxidant multienzyme, in preparation of medicines or health-care foods for treating rheumatoid arthritis and osteoporosis. In the corn germ concentrated powder, superoxide dismutase / (U / g) is greater than or equal to 8500, glutathione peroxidase / (U / g) is greater than or equal to 5000, and catalase / (U / g) is greater than or equal to 750. The corn germ concentrated powder can also be prepared by the following method: cleaning corn, germinating, adding water into the germinated corn, grinding and pulping, adding a settling agent into the corn steep liquor, standing, taking supernate, adding part of sodium ascorbate, performing temperature-controlled concentration, adding the rest sodium ascorbate and a stabilizer into the concentrated solution, and uniformly mixing to obtain the corn germ concentrated powder. And drying at controlled temperature, crushing, and mixing with a proper amount of silicon dioxide. The prepared corn germ concentrated powder has the effect of resisting rheumatoid arthritis.

The present application discloses a filamentous fungal mutant strain with high-efficient expression of superoxide dismutase and its application. The filamentous fungal mutant strain of the present application is formed by gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 in the wild-type filamentous fungus Podospora anserina. The filamentous fungal mutant strain of the present application is a mutant strain with gene knockout of phytochrome gene PaPhy1, a mutant strain with gene knockout of phytochrome gene PaPhy2, or a mutant strain with gene knockout of phytochrome genes PaPhy1 and PaPhy2. The present application first discovers that the filamentous fungus Podospora anserina with gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 can express superoxide dismutase and peroxidase more efficiently, which is of great significance for the high-efficient expression and preparation of superoxide dismutase and peroxidase.

The invention relates to the technical field of biology, and discloses an efficient extraction process of SOD in silphium perfoliatum, which comprises the following steps: mixing a silphium perfoliatum superoxide dismutase crude extract, polyethylene glycol and a first salt, and adjusting the pH value to form an aqueous two-phase system to enrich SOD; adding polysaccharide hydrolase and protease into the system to carry out in-situ enzymolysis so as to degrade macromolecular impurities; after the enzymolysis is finished, adding a second salt for competitive distribution, and selectively removing the added functional enzyme to a salt phase; and finally, separating and collecting a supernatant phase rich in superoxide dismutase. By integrating aqueous two-phase extraction, in-situ enzymolysis and competitive distribution into a single reaction system, a tedious intermediate separation unit in a traditional process is replaced, and by continuous and targeted removal of endogenous macromolecular impurities and exogenous functional enzymes, the purity and specific activity of a final product are remarkably improved, so that the method is suitable for industrial production. And the biological activity of SOD is effectively protected by the mild separation conditions in the whole process.

The invention belongs to the technical field of biological medicines, and particularly relates to an MXene nano material with high SOD (superoxide dismutase) enzyme activity as well as a preparation method and application thereof. The MXene nano material with high SOD (superoxide dismutase) enzyme activity, disclosed by the invention, comprises Mo2C MXene and a metal atom anchored on the Mo2C MXene. The MXene nano material with high SOD enzyme activity has the beneficial effects that the MXene nano material with high SOD enzyme activity has relatively high SOD enzyme activity and a strong ROS removal characteristic, and oxidative stress injury can be relieved; the compound has the effect of treating ROS related diseases such as traumatic brain injury (TBI), Parkinson's syndrome (PD) and chronic obstructive pulmonary disease.

The invention discloses an application of recombinant human copper / zinc superoxide dismutase in preparation of a medicine for treating triple negative breast cancer. The amino acid sequence of the recombinant human copper / zinc superoxide dismutase is shown as SEQ ID NO.1. The invention further discloses a preparation method of the recombinant human copper / zinc superoxide dismutase. The medicine for treating the triple negative breast cancer contains recombinant human copper / zinc superoxide dismutase. The medicine for treating the triple-negative breast cancer, which is prepared by recombining and transforming the human copper / zinc superoxide dismutase, can effectively control in-vitro migration of a triple-negative breast cancer cell line and tumor growth of a triple-negative breast cancer tumor-bearing mouse, so that the lifetime of the tumor-bearing mouse is effectively prolonged; therefore, the medicine for treating the triple-negative breast cancer has a very positive effect on treatment of the triple-negative breast cancer.

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

The invention provides two-dimensional bimetal organic framework nano-enzyme hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological materials. According to the preparation method, a FeCuMOF bimetal organic framework is adopted as a nano-enzyme, tannic acid and lipoic acid are used for constructing a composite hydrogel system with antibacterial and antioxidant capacities, and the composite hydrogel is combined with FeCuMOF to form the two-dimensional bimetal organic framework nano-enzyme hydrogel. The two-dimensional bimetal organic framework nano-enzyme hydrogel disclosed by the invention has antibacterial and anti-biofilm properties, and can be used for removing various ROS (Reactive Oxygen Species) by simulating superoxide dismutase and the capability of removing ABTS free radicals thereof. In addition, proinflammatory cytokines can be reduced, and anti-inflammatory cytokines can be increased, so that periodontitis inflammation is relieved, and tissue regeneration is accelerated. The two-dimensional bimetal organic framework nano-enzyme hydrogel disclosed by the invention is used as a multifunctional nano-enzyme hydrogel slow-release platform, and an ideal strategy is provided for treating periodontitis.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.