715 results about "Superoxide dismutases" patented technology

The invention discloses a Pichia kluyveri yeast and its application. The Pichia kluyveri yeast is classified and named as Pichia kluyveri JLG30201, which has been deposited in the China Center for Type Culture Collection, with a deposit number of CCTCC NO: M 20242295 and a deposit date of October 22, 2024. The Pichia kluyveri JLG30201 has strong killer affinity and high sugar tolerance, unique aroma production, high production of extracellular superoxide dismutase (SOD), and low alcohol production; the strain is applied to the processing of fruit wine or edible plant enzymes, and has the effects of increasing ester and flavor, improving juice yield and product antioxidant activity.

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to a composite hydrogel dressing for promoting wound healing as well as preparation and application thereof, and belongs to the technical field of biological medicines. The hydrogel disclosed by the invention is prepared by mixing gelatin, oxidized dextran and metal ion modified metal-organic framework compounds (MOF-Ag and MOF-Cu). In the mixed solution, amino groups in the gelatin and aldehyde groups of the oxidized dextran are subjected to a Schiff base cross-linking reaction, and the composite hydrogel is formed after standing. The loaded MOF-Ag has antibacterial and anti-biofilm effects on staphylococcus aureus and escherichia coli, and the loaded MOF-Cu has similar superoxide dismutase activity and similar glutathione peroxidase activity, so that excessive active oxygen can be removed, and inflammatory response can be relieved. The obtained hydrogel has good biocompatibility, proper mechanical properties and biological adhesion, stable rheological properties, excellent antibacterial and anti-inflammatory functions and a certain hemostatic function.

The invention provides a nano transdermal delivery system based on superoxide dismutase, the nano transdermal delivery system is of a capsule composite structure of a small molecule active component-superoxide dismutase-polymer shell layer, the small molecule active component is loaded in superoxide dismutase, the surface of the superoxide dismutase is coated by the polymer shell layer, and the superoxide dismutase is loaded in the capsule composite structure. The diameter of the superoxide dismutase-based active ingredient transdermal delivery system is 20-100 nm, and the thickness of the polymer shell layer is 7.5-52.5 nm. In the delivery system, the polymer shell layer provides a protection effect for the superoxide dismutase and the micromolecular functional substances with the anti-oxidation effect, the capsule composite structure is in a nanogel form, and the nanogel not only promotes the penetration depth of the capsule, but also reduces the irritation effect of the capsule on the skin. The raw materials are biologically friendly, the preparation method is simple, the yield is high, and industrial large-scale production can be realized.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a tumor cholesterol metabolism regulation microneedle patch and a preparation method, the microneedle patch comprises a needle tip and a backing which are connected, the needle tip comprises a needle tip body and nanoparticles loaded on the needle tip body, the nanoparticle is a manganese ion-doped organic metal framework, the outer layer of the manganese ion-doped organic metal framework is modified with a targeting agent, cholesterol oxidase and superoxide dismutase are carried on the manganese ion-doped organic metal framework, the targeting agent can target a CD44 receptor, and the organic metal framework can carry drugs. The targeted drug can enter tumor cells in a targeted mode, superoxide dismutase catalyzes superoxide anions overexpressed in the tumor cells to generate H2O2 and O2, O2 needed by catalysis is provided for cholesterol oxidase, cholesterol at the tumor site is consumed by the cholesterol oxidase, accumulation of 7-DHC is reduced, meanwhile H2O2 can be generated, and the cholesterol oxidase can be used for catalyzing the tumor site. H2O2 is catalyzed by manganese ions through a Fenton-like reaction to generate OH to induce tumor cells to generate ferroptosis, so that ferroptosis-immune synergistic treatment is realized.

The invention relates to the field of biomedical materials, and particularly discloses a manganese dioxide nano-enzyme for treating inflammatory bowel disease as well as a preparation method and application of the manganese dioxide nano-enzyme. The preparation method of the manganese dioxide nano-enzyme comprises the following steps: uniformly mixing an organic solution containing manganese nitrate hexahydrate with a montmorillonite aqueous solution to obtain a mixed reaction solution; the pH value of the mixed reaction solution is adjusted to be under the alkaline condition through alkali liquor, a synthetic reaction is carried out, then centrifugation and washing are carried out, the montmorillonite-loaded manganese dioxide nano-enzyme is obtained, and the manganese dioxide nano-enzyme is of a lamellar structure with the length being 20-400 nm. The manganese dioxide nano enzyme has excellent activity similar to superoxide dismutase, catalase and glutathione peroxidase; meanwhile, the compound also has the capability of removing various free radicals such as DPPH and ABTS, can be used for treating the inflammatory bowel disease, and has a wide application prospect.

The present invention relates to magnetic, catalytically active, and radiosensitizing nanoparticles (NanoXcats) and the method for their production. A method is disclosed for the production of functionalized greigite nanoparticles with a narrowband size distribution (10-30 nm) for use in multimodal cancer therapy. NanoXcats are superparamagnetic Fe3S4 nanoparticles whose surfaces are equipped with polar functional groups. The surface structures of NanoXcats are suitable for conjugation with, or for covalent or electrostatic binding of, active substances, which may be cancer drugs, cell growth inhibitors, or NO donors. Due to the EPR effect, NanoXcats are preferentially internalized by tumor cells. The targeted accumulation of NanoXcats in tumor tissue is achieved by an external inhomogeneous magnetic field. NanoXcats exhibit enhanced catalytic activity in tumor cells.The catalytic activity of intracellular NanoXcats can be triggered, controlled, and enhanced by low-dose X-rays. Surface-bound drugs are additionally activated in tumor cells by the catalytic activity of NanoXcats. NanoXcats act as superoxide dismutase, nitric oxide synthase, and Fenton catalyst. They catalyze, for example, reactions of the superoxide anion radical to hydrogen peroxide, of L-arginine to NO, and of hydrogen peroxide to the hydroxyl radical.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention provides a polypeptide and application thereof in preparation of a medicine for preventing and / or treating alcoholic liver injury. The polypeptide is safe and free of side effects, can remarkably reduce the content of malondialdehyde (MDA), improve the activity of superoxide dismutase (SOD), improve the content of glutathione (GSH), reduce the activity of glutamic-pyruvic transaminase (ALT), reduce the activity of aspartate transaminase (AST) and reduce the activity of lactic dehydrogenase (LDH), and is very suitable for preparing the medicine for preventing and / or treating the alcoholic liver injury.

The invention relates to a skin care composition containing white truffle and plant exosome and application thereof. The skin care composition containing white truffle and plant exosome comprises white truffle extract, chlorella exosome, DNA sodium, superoxide dismutase and fermentation product filtrate, the fermentation product filtrate comprises any one or a combination of at least two of lactobacillus / soybean milk fermentation product filtrate, lactobacillus / mung bean seed extract / sodium glutamate fermentation product filtrate, bifidobacterium fermentation product filtrate or saccharomycetes / rice fermentation product filtrate. According to the invention, the white truffle extract, the chlorella exosome, the DNA sodium, the superoxide dismutase and the fermentation product filtrate are creatively combined, and the composition has unexpected synergistic interaction in the aspects of inhibiting melanin synthesis, accelerating melanin metabolism and resisting oxidation and aging. The composition is mild in performance and free of irritation and toxic and side effects, and can be applied to cosmetic products more safely.

The invention discloses parabacteroides faecium capable of relieving senescence and application of the parabacteroides faecium, and belongs to the technical field of microorganisms and the technical field of medicines. Animal experiments prove that Parabacteroides vervae CCFM1428 has the effects of improving the weakness index of a mouse, increasing peroxide dismutase in the liver of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px), increasing the level of catalase, reducing an inflammatory factor IL-6, reducing an inflammatory factor TNF-alpha and increasing the level of acetylcholine in the brain of the mouse, and has the effects of improving the weakness index of the mouse, increasing the liver peroxide dismutase of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px) and the level of catalase. The gripping force of the mouse is improved. The strain disclosed by the invention has a huge application prospect in preparation of products for relieving senescence.

The invention discloses application of burdock root polysaccharide in preparation of a medicine for treating cognitive impairment, and belongs to the technical field of traditional Chinese medicines. Experiments prove that the burdock root polysaccharide can improve cognitive impairment and autonomous exploration ability of D-galactose induced cognitive impairment model mice, increase the activity of superoxide dismutase in serum of the mice, reduce the content of malondialdehyde, reduce the content of hippocampus interleukin-1beta and tumor necrosis factor-alpha in brains of the mice, and improve the cognitive impairment activity of the mice. According to the present invention, with the composition, the loss of the neuron in the hippocampal region of the cognitive impairment model mouse can be reduced so as to achieve the neuron protection effect, such that the good treatment effect on the cognitive impairment can be provided, and the new approach is provided for the development of the cognitive impairment-related drug.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention relates to ultra-small prussian blue nano-particles (T-USPB-C) carrying tirofian, a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for reducing MVO and MIRI. According to the preparation method, the T-USPB-C is prepared by three steps. The T-USPB-C provided by the invention can carry tirofiban, and has catalase and superoxide dismutase simulated nano-enzyme activity. Moreover, the size of the nano-scale drug is required to reach a nano-scale size, and the drug can be efficiently cleared through kidney excretion, so that the potential long-term toxicity problem is minimized. The ultra-small prussian blue nanoparticle carrying the tirofian has an excellent active oxygen scavenging capability. Microvascular perfusion can be rapidly improved through the antithrombotic effect, then the protection effect is continuously achieved through the anti-oxidation and anti-inflammatory mechanism, and microvascular injury and MIRI are effectively prevented.

The invention provides a tissue protection solution and application thereof. The tissue protection liquid comprises a basic culture medium; vitamins, wherein the vitamins comprise vitamin E; the antioxidant is prepared from at least one of N-acetylcysteine amide, superoxide dismutase and catalase; and a specific cell factor, wherein the specific cell factor comprises at least one of a ROCK inhibitor Y27632 and insulin. Therefore, when the tissue protection liquid is used for storing and transporting a tissue sample, the loss in the process from tissue sample separation to experiment starting can be reduced, the function and activity of tissue cells are ensured, and the success rate of establishment of various primary tissue cultures is effectively improved.

The invention belongs to the technical field of catalytic materials, and particularly relates to high-entropy alloy nanoparticles used as a biocatalyst and application of the high-entropy alloy nanoparticles. The PtFeCuCoNi high-entropy alloy nano-particles with pH adaptability are developed through a one-step solvothermal synthesis method, it is found that the PtFeCuCoNi high-entropy alloy nano-particles can serve as potent oxidase, can generate reactive oxygen species (ROS) and effectively kill bacteria such as MRSA under the acidic condition, and can play the functions of superoxide dismutase and catalase under the neutral condition, so that the PtFeCuCoNi high-entropy alloy nano-particles with the pH adaptability can be used for effectively killing bacteria such as MRSA. Active oxygen in cells is efficiently removed, oxidative stress is relieved, and the cell protection effect is achieved.

The invention provides a plant lactobacillus IOB602 for improving the protection mechanism of retinal cells under blue light and application of the plant lactobacillus IOB602, and relates to the technical field of microorganisms. The lactobacillus plantarum is classified and named as lactobacillus plantarum, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.16021. The strain improves the activity of retinal epithelial cells, reduces the content of cell proinflammatory factors IL-6, IL-1beta and TNF-alpha, relieves inflammation, is beneficial to recovery of the expression of antioxidant enzymes, and effectively improves macular region edema by reducing the content of vascular endothelial growth factors; the activity of catalase, superoxide dismutase and glutathione peroxidase is improved; the content of proinflammatory factors IL-6, IL-1beta and TNF-alpha in mouse serum is reduced, and the immune system of a body is regulated, so that the protection mechanism of retinal cells in a blue light environment is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus plantarum (Lactobacillus plantarum) and an application of the lactobacillus plantarum. The preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.24756. The lactobacillus plantarum has good DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging capacity, hydroxyl radical scavenging capacity and reducing capacity, and can remarkably reduce the level of malondialdehyde (MDA) as an oxidative stress marker in serum and liver of an oxidative stress body, the pathological damage level of liver tissue and the level of inflammatory factors in serum; the level of an antioxidant substance glutathione (GSH) in serum and liver of an oxidative stress body and the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in the serum and liver can be remarkably improved. In addition, fire extinguishing bacteria of the lactobacillus plantarum have more excellent antioxidant activity than viable bacteria.

The invention relates to the technical field of computers, in particular to a parameter optimization method and system for processing a skin abnormal area through SOD, according to the method, a reference skin area image can be flexibly selected according to an actual application scene, an SOD basic parameter vector used for assisting SOD component parameter adjustment can be determined through an image analysis and data processing process, and the SOD basic parameter vector can be used for assisting SOD component parameter adjustment. The SOD basic parameter vector does not need to be obtained through an experiment mode, the application scene of the SOD basic parameter vector can be flexibly adjusted by replacing a plurality of processed reference skin area images, so that the SOD component parameter optimization can be carried out by taking the SOD basic parameter vector as a reference, and the SOD component parameter optimization efficiency is improved. Compared with the prior art that experiments are carried out by traversing the SOD component parameters, the method has the advantages that the number of experiments in the SOD component research process can be reduced, so that the efficiency of optimizing the SOD component parameters is improved, corresponding SOD basic parameter vectors can be provided for different scenes, and the efficiency of optimizing the SOD component parameters is further improved.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum fermentation liquor and application thereof. The lactobacillus plantarum fermentation liquor provided by the invention is obtained by taking roses as a substrate and fermenting screened plant milk stalks under specific conditions. Experimental results show that the content of effective active ingredients such as total polyphenol, total polysaccharide and superoxide dismutase in rose fermentation liquor fermented by the lactobacillus plantarum is relatively high, and the fermentation effect of the rose fermentation liquor is far superior to that of commercially available common lactobacillus plantarum and other strains; besides, the rose fermentation liquor also shows obvious DPPH free radical and hydroxyl free radical scavenging ability, can effectively inhibit the activity of tyrosinase and improve the cell viability, has anti-aging and whitening effects, and can be widely applied to various foods and skin care products.

The invention provides a composition with a function of stabilizing skin enzyme activity as well as a preparation method and application of the composition, and the composition is prepared from the following components in parts by mass: 0.001 to 0.01 part of superoxide dismutase, 0.001 to 0.05 part of nicotinamide adenine dinucleotide, 0.11 to 12 parts of a polypeptide compound a and 0.005 to 0.5 part of a polypeptide compound b. The invention also provides a preparation method of the composition and application of the composition in preparation of skin care products for improving skin enzyme activity, repairing skin and resisting aging. The composition can be prepared into emulsion, solution, paste, gel or spray. The composition can effectively improve the enzyme activity of skin, improve the texture and appearance of the skin and repair the skin, and is beneficial to delaying the aging of the skin at the same time.

The invention discloses a construction method of an anti-oxidation adhesion type genetically engineered bacterium, the engineering bacterium and application of the engineering bacterium, and relates to the technical field of construction of the anti-oxidation adhesion type genetically engineered bacterium. According to the invention, superoxide dismutase and catalase are subjected to constitutive overexpression in a probiotic chassis by using a synthetic biological technology, and meanwhile, intestinal trefoil factor peptide fused pilin is subjected to overexpression. According to the present invention, the research results show that the active oxygen scavenging ability of the anti-oxidation adhesion type gene engineering probiotic is significantly improved, the adhesion ability of the anti-oxidation adhesion type gene engineering probiotic in the animal intestinal tract is significantly enhanced, and the radiation enteritis symptom caused by the whole abdomen ionizing radiation irradiation can be effectively relieved; the strain can be used as a potential safe viable bacterium medicine for preventing and treating radiation enteritis and other active oxygen related enteritis. The anti-oxidation adhesion type genetic engineering probiotics can be prepared into viable bacteria preparations such as viable bacteria suspension, dairy products, viable bacteria powder, bacterial tablets or capsules, can be proliferated and colonized in human intestinal tracts after being orally taken, and is applied to prevention and treatment of radiation enteritis.

The invention provides an SOD (superoxide dismutase) and CAT (catalase) double-enzyme-activity fusion protein as well as a construction method and application thereof, and belongs to the technical field of mixed enzymes. The invention provides a fusion protein of SOD (superoxide dismutase) enzyme and CAT (catalase) enzyme connected by a connecting peptide. The invention provides a recombinant expression vector and a recombinant expression method for expressing the fusion protein, and also verifies that the fusion protein has relatively strong stability and acid-base tolerance after being expressed, and the fusion protein has relatively high SOD enzyme activity and CAT enzyme activity. The fusion protein provides a new choice for combined application of SOD and CAT, and can be widely applied to food, beauty and biological medicine industries.

The invention relates to the technical field of biological medicine, and discloses a culture medium for human metapneumovirus and application, the culture medium comprises the following components by mass: 850-920 parts of a basic culture medium; the metabolism reprogramming module is prepared from the following components in parts by weight: 0.05 to 0.15 part of 5-methyltetrahydrofolic acid, 0.3 to 0.7 part of N-acetyl-D-mannosamine and 1.0 to 3.0 parts of sodium dichloroacetate; the immune regulation and control module is prepared from 0.005 to 0.015 part of an RIG-I / MAVS pathway inhibitor; the receptor induction module is prepared from 0.5 to 1.5 parts of all-trans retinoic acid; the redox balance module is prepared from the following components in parts by weight: 0.1 to 0.5 part of pegylated superoxide dismutase and 0.002 to 0.008 part of sodium selenite. According to the method, the proportion of infectious virus particles can be remarkably increased, the generation of defective particles is reduced, meanwhile, the production cycle of host cells is prolonged, large-scale continuous culture is supported, and a high-quality and high-efficiency solution is provided for industrial production of gene therapy vectors and virus vaccines.

Provided is a superoxide dismutase-based nanoscale transdermal delivery system, which is a capsule composite structure consisting of a small molecule active ingredient, superoxide dismutase, and a polymer coating. The small molecule active ingredient is loaded in the superoxide dismutase, and the surface of the superoxide dismutase is coated with the polymer coating. The superoxide dismutase-based active ingredient transdermal delivery system has a diameter of 20-100 nm, and the polymer coating has a thickness of 7.5-52.5 nm. In the delivery system, the polymer coating protects the superoxide dismutase and the small molecule functional substance having an antioxidant effect, and the capsule composite structure is in the form of nanogel. The nanogel not only promotes the penetration depth of the delivery system, but also mitigates the stimulation of the delivery system to the skin. The delivery system features biofriendly starting materials, simple preparation method, and high yield, and thus can be produced in large scale industrially.

The invention relates to a probiotic stock solution capable of producing folic acid and SOD (superoxide dismutase) and having the functions of reducing uric acid and reducing blood sugar and a preparation method of the probiotic stock solution. Existing probiotic products are inconvenient to eat and do not have the effects of producing folic acid and SOD (superoxide dismutase). The probiotic stock solution contains the bifidobacterium animalis fermentation liquor, the lactobacillus paracasei fermentation liquor, the lactobacillus casei fermentation liquor, the lactobacillus fermentum fermentation liquor, the lactobacillus rhamnosus fermentation liquor and the lactobacillus reuteri fermentation liquor, and the final product is in a liquid state, can be packaged by small strip bags, and is relatively convenient to refrigerate, store and use. The probiotic stock solution disclosed by the invention not only has the effects of reducing uric acid and reducing blood sugar, but also generates water-soluble vitamin folic acid and superoxide dismutase SOD in the preparation and fermentation process, has the effects of resisting oxidation and aging after being taken into a human body, is suitable for pregnant women to take, and can reduce the risk of fetal neural tube defect.

The invention discloses a method for preparing recombinant human copper-zinc superoxide dismutase. The method comprises the following steps: (a) designing and synthesizing a gene sequence for coding the recombinant human copper-zinc superoxide dismutase; (b) cloning the gene sequence into an expression vector to construct a recombinant expression vector; (c) transforming the recombinant expression vector into an expression host cell to obtain a recombinant strain; (d) inducing the expression host cell to express the recombinant human copper-zinc superoxide dismutase under proper conditions; and (e) collecting an expression product, and carrying out multi-step purification to obtain the high-purity recombinant human copper-zinc superoxide dismutase. According to the superoxide dismutase provided by the invention, the expression quantity and activity of recombinant protein are remarkably improved, the purification process is simplified, the production cost is reduced, and meanwhile, the high-quality standard of a product is ensured.