742 results about "Superoxide dismutases" patented technology

The invention is concerned with a kind of Rosa roxburghii Tratt candy produce. It produces the syrup of pure Rosa roxburghii Tratt or compound Rosa roxburghii Tratt into horniness candy, horniness candy with filling, soft candy, milk lipid candy, polishing candy, glue-based candy, charging candy, chocolate candy or jelly, and so on. It increases the stability of vitamin C, vitamin P and superoxide dismutase and affords a kind of high quality candy food with double functions of nutritional health care and relaxing. The produce method relates to prepare clarified Rosa roxburghii Tratt concentrated syrup with good stability, add assistant stuff as the amount of 1 to 50 percent of whole amount into the said syrup, prepare Rosa roxburghii Tratt grain or Rosa roxburghii Tratt fast-dissolving powder and follow the routine technology to produce horniness candy, horniness candy with filling, gel candy, glue-based candy, charging candy, chocolate candy or jelly, and so on.

The invention discloses a preparation method of a mushroom ferment beverage with effect of improving immunity, and belongs to the technical field of food beverages. According to the method disclosed by the invention, mushrooms and/or medicinal plants, fruit and ginseng are adopted as raw materials. The method comprises the following steps: slicing, chopping or crushing the raw materials; uniformly mixing the sliced, chopped or crushed raw materials so as to obtain a mixture; after adding sugar in the mixture, fermenting the mixture in ferment microorganisms; adding sugar when the sugar content is lowered; continuing fermentation so as to obtain the mushroom ferment beverage. According to the method disclosed by the invention, ferment bacteria can regulate intestinal flora and generate SOD (superoxide dismutase), so that the raw materials can be successfully fermented; the prepared ferment beverage contains protease, lipase, SOD, pectinase, lucid ganoderma, cauliflower fungus, pleurotus eryngii polysaccharide, rare ginsenoside, Rb2, C-K and the like. According to the method disclosed by the invention, the fermentation liquor and mycelium of the mushrooms can be directly used for fermentation; compared with sporophore, the raw materials are easier to obtain, and the method has the advantages of being short in fermentation cycle, easy in formula, simple in fermentation process, and the like.

The invention relates to blood lipid-lowering active fractions of forsythia suspensa and a preparation method and use of the same. The active fractions are ethyl acetate fractions obtained by concentrating an extract which is extracted from forsythia suspensa leaves by acetone and water. The compound of phillygenin prepared by the invention and the active fractions, namely the ethyl acetate fractions, at which the phillygenin is positioned have good antioxidant activity in vitro. In-vivo experiments show that the ethyl acetate fractions and the phillygenin can lower total cholesterols and low-density lipoprotein cholesterin in the blood serum of a mouse with hyperlipidemia and can lower blood lipid level by an oxidative approach (raising superoxide dismutase SOD and lowering malonydialdehyde MDA). In addition, the ethyl acetate fractions have a better effect of reducing the total cholesterols in the blood serum of the mouse than the phillygenin and cassia seed blood lipid-lowering tablets. The ethyl acetate fractions of the forsythia suspensa leaves can be further developed to play a role in the multi- target co-prevention and treatment of hyperlipidemia and other diseases and have a good application prospect.

The invention discloses river crab feed, which comprises fish meal, vegetable dregs, corn, soya bean meal, inferior meal, soya-bean oil, shrimps, cuttlefish ointment, monocalcium phosphate, mineral substances for crabs, vitamin for the crabs, a binder, 0.7 to 14.0 mg/kg of cordycepin, 131.0 to 2,620.0 mg/kg of cordycepic acid, 0.1 to 2.0 mg/kg of adenosine and 290.0 to 5,800.0 IU/kg of superoxide dismutase. The river crab feed has the high utilization rate, reduces the pollution of feed residues to environment, avoids the negative effects of antibiotic residues and tolerance caused by the antibiotic residues, is safe and harmless to the craps and the environments and can enhance the immunity of the crabs and accelerate the growth of the crabs. The invention also provides a production process of the river crab feed, which has the characteristics of low cost and simple and convenient operation.

The invention discloses a platinum nano-material with the activity of four mimic enzymes and a preparation method thereof. The preparation method comprises the following steps: using citrate ions as a modifier, reducing chloroplatinic acid by utilizing sodium borohydride, and preparing the platinum nano-material modified by citric acid. The platinum nano-material is simply and quickly prepared, the particle size is small, and the dispersibility and stability of a water solution are high. The obtained platinum nano-material has the activity of four mimic enzymes at the same time, and comprises peroxidase, oxidase, catalase and superoxide dismutase. The activity of the four mimic enzymes of the platinum nano-material can be adjusted and controlled through the concentration of the citrate ions used in the preparation process.

There is provided use of a compound in the manufacture of a medicament to inhibit superoxide dismutase (SOD) or for use in the therapy of a condition or disease associated with SOD wherein the compound is of Formula I wherein: X is a ring having at least 4 atoms in the ring; K is a hydrocarbyl group; R<1 >is a halogen or a group of the formula -L<1>-Z-R<1>, wherein L<1 >is an optional linker group, Z is O or S and R<1 >is a hydrocarbyl group or H; with the proviso that the compound is other than 2-methoxy-17beta-oestradiol, 2-methoxyoestrone and 2-hydroxyestradiol.

The invention discloses a blueberry SOD (Superoxide Dismutase) red wine and a preparation method thereof. The blueberry SOD red wine comprises 75ml of blueberry primary extraction solution, 100,000-200,000 units of blueberry SOD crude enzyme and 675ml of red wine. The preparation method of the blueberry SOD red wine comprises the following steps of: (1) cleaning blueberry; (2) draining water on the surface of the blueberry; (3) crushing and pulping by using a crushing and pulping machine, adding one time of volume of 4DEG C de-ionized water and carrying out ultrasound crushing at the constant temperature for 2 hours; (4) centrifuging to obtain supernate as the primary extraction solution; (5) intercepting the primary extraction solution to obtain a concentrated solution with the molecular weight of 20,000-60,000; (6) adding fucose as an SOD protecting agent and carrying out vacuum freezing and drying to obtain the blueberry SOD crude enzyme; and (7) mixing the blueberry SOD crude enzyme with the primary extraction solution, then adding the mixture into the red wine, sterilizing and bottling. The blueberry SOD wine obtained by the preparation method disclosed by the invention has the advantages of abundant nutrient components, favorable mouth feeling and high SOD survival rate.

The invention provides a SOD gene from animal and a method for cloning and expressing the SOD gene in colibacillus and yeast cells, wherein a nucleotide sequence of superoxide dismutase is shown as SEQ NO.1; an amino acid sequence of the superoxide dismutase is shown as SEQ NO.2; carriers of nucleotide molecules are colibacillus plasmids or yeast plasmids; cells of the nucleotide molecules are formed by carrier conversion; and cells of the nucleotide molecules of the superoxide dismutase contain colibacillus containing the nucleotide molecules or converted by the carriers or pichia yeast containing the nucleotide molecules or converted by the carriers. The invention can prepare recombinant production strains which can efficiently express and secrete Cu/Zn-SOD, realizes the production industrialization of the Cu/Zn-SOD, and achieves good pH stability, favorable thermal stability and Cu/Zn-SOD products with anti-protease hydrolyzation capacity.

The invention achieves control over mixing controlled atmosphere storage of fruit vegetables by preparing an air-conditioned cold store, selecting fruits and vegetables, precooling, and stacking. Fruits and vegetables, upon pickup. are precooled to 9-11° C. in a vacuum pre-cooler for 12-24 hours. They are then stacked in a controlled atmosphere storage house. O₂, CO₂, N₂ and Ar with different proportions are used for modified atmosphere packaging. The invention can extend the storage of mixing storage of various kinds of typical fruits and vegetables to more than 50 days, during which the fruits and vegetables are maintained in good color and flavor and have higher peroxidase, superoxide dismutase, catalase and cellulose activity; also, accumulation of hazardous substances such as superoxide anion free radical, hydrogen peroxide and malonaldehyde is effectively reduced, the retention rate of Vc is up to 80%-90%, and the weight loss ratio can be controlled to be below 8%.

The invention discloses a method detecting activity of plant superoxide dismutase, catalase and peroxidase, which comprises the steps of grinding a sample by using a liquid nitrogen method, extracting three enzymes simultaneously by adopting one buffer solution, detecting enzyme activity and the like. In the method, SOD, CAT and POD in plants can be simultaneously extracted by adopting one buffer solution, so that the method fulfills the aim of simply and quickly detecting the enzyme activity of the SOD, CAT and POD, simplifies operation, saves time and labor, reduces the using amount of samples and reagent and the like, and contributes to the realization of batch operation, and the detection result is similar to that of the conventional method. Furthermore, the sample is ground by adopting a liquid nitrogen grinding method before enzyme solution is extracted, so that the method has the advantages of high grinding speed, good cell crushing effect and high extraction efficiency.

The invention discloses a cultivation method for a fruit tree bearing fruit rich in superoxide dismutase. The cultivation method is a planting method which employs one or more microbial preparations to produce fruit rich in copper-zinc superoxide dismutase (Cu/Zn-SOD). Compared with the prior art, the cultivation method provided by the invention has the advantage that the content of SOD is greatly increased. By eating the fruit, the health conditions of people can be greatly improved; elimination of sub-health can be facilitated; immunity can be enhanced; excess superoxide anion free radicals in human bodies can be scavenged; and occurrence of a plurality of diseases can be prevented.

The invention relates to a preparation method for synthetizing a NaA type molecular sieve membrane in seed crystal aerosol. An out-of-phase SOD (Superoxide Dismutase) type molecular sieve seed crystal is adopted as induction to synthetize a nano NaA type molecular sieve crystal in fluorine-containing aerosol, a support modified by a silane coupling imidazolium cation based ionic liquid ethanol solution is put in synthesis aerosol containing the nano NaA type molecular sieve crystal, and a compact and continuous NaA type molecular sieve crystal layer is formed on the surface of the modified support by adopting a hydrothermal synthesis method. The surface of the prepared NaA type molecular sieve crystal membrane is continuous and compact, the separation performance is good and the repeatability is good, and the method is suitable for being used in large-scale preparation of the NaA type molecular sieve crystal membrane and application of industrial solvent dehydration of the NaA type molecular sieve membrane.

The invention provides a coproduction process for extracting various bioactivators from pig placenta, which comprises the following steps: taking the pig placenta (containing farrowing blood) as a raw material, utilizing components rich in the raw material such as immune globulin (Ig), immunocompetent peptide, superoxide dismutase (SOD), lipopolysaccharide, placental protein and the like, and applying modern biochemistry technology and biological integration technology such as salting in, salting out, chromatography, ultra-filtration and the like according to different structures, molecular weights, isoelectric points, physical and chemical properties, biological activities of the components to make the coproduction process suitable for industrial production. Therefore, in the same flow of the production process, the effective components in the pig placenta with market development value can be produced, namely five products of the immune globulin, the immunocompetent peptide, the superoxide dismutase, the lipopolysaccharide and the placental protein. The process combines the conventional and modern processes, produces various products through coproduction, and not only makes full use of placenta resources to change the waste into the treasure, does not discharge waste liquid and prevents the environment, but also can greatly reduce the production cost and improve the economic benefit.

The invention discloses a hydrolat moisturizing facial mask. The method comprises the following steps: putting a hydrolat with moisturizing efficacy into a container, then adding hyaluronic acid, fish collagen, water soluble vitamin and lauric acid modified superoxide dismutase into the container, and stirring the solution until the solution is completely dissolved; then adding aloe extracting solution and supguard GM-BP preservative into the solution, and uniformly stirring, uniformly dripping the obtained liquid onto a non-woven fabric facial mask, then conducting sealed packaging to obtain a product, namely the hydrolat moisturizing facial mask. The hydrolat with moisturizing efficacy includes rose hydrolat, witch hazel hydrolat, jasmine hydrolat, marigold hydrolat, sandalwood hydrolat and the like, and any one of the hydrolat or combination of any two or more of the hydrolat can be adopted. The hydrolat moisturizing facial mask prepared by adopting the method disclosed by the invention has the advantages that the moisturizing effect is prominent, and the mask is safe and can not irritate a human body, and the like.

The invention provides a method for cultivating wheat seedlings by using nitrogen stress nutrient solution. The method comprises the following steps of: repeatedly soaking wheat seeds in nitrogen stress solution until the wheat seeds sprout and grow to the height of between 5 and 20cm; and accumulating active oxygen in cells of the wheat seedlings by nitrogen stress to increase the quantity and activity of protective enzyme and enrich a polyphenolic substance. In order to prevent excessive active oxygen from damaging the cells, a method for alternating repeated and short-term nitrogen stress and nutrient solution cultivation is adopted to make the active oxygen slowly generated. By the method, more superoxide dismutase, catalase, peroxidase and the polyphenolic substance can be obtained, the yield of the wheat seedlings is improved, and new species of wheat seedlings rich in antioxidants are produced.

The invention discloses a composition with skin-whitening and anti-ageing effects, a skin care product containing the composition as well as a preparation method and an application of the composition. The composition is prepared from the following components: 0.2%-10.0% of L-ascorbic acid 2-glucoside, 0.1%-0.2% of superoxide dismutase, 0.1%-0.5% of a grape seed extract, 0.1%-0.5% of a soybean fermentation extract, 10%-30% of propanediol, 0.1%-0.2% of aloe powder and the balance amount of water. An experimental result shows that the composition has the skin-whitening and anti-ageing effects, the method is easy in operation, the production cost is low, and the product stability is high; an evaluation on an in vitro test and a human body skin test proves that the composition has good skin-whitening and anti-ageing effects and is safe and free from side effects; meanwhile, the composition and the skin care product containing the composition, provided by the invention, also have better long-lasting hydrating effects.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

The invention discloses a method for preparing superoxide dismutase (SOD)-loaded gamma-poly glutamic acid hydrogel. The method comprises the following steps: adopting a 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) activation system, and forming an SOD-gamma-polyglutamic acid (PGA) immobilized product by using lysine residues and poly glutamic acid side chain carboxyl of SOD; crosslinking the SOD-gamma-PGA obtained by the reaction by using a diamine crosslinking agent cysteamine hydrochloride, so as to form an SOD-gamma-PGA gel system. The gel system can be applied to wound dressing or mask, and can play the antioxidant damage action, and the skin wound is quickly repaired.

The invention relates to a wine and a preparation method thereof, and in particular relates to a wine containing an SOD (Superoxide Dismutase) component and a preparation method thereof. The preparation method of the wine containing the SOD component comprises the following steps of: (1) preparing phyllanthus emblica wine juice; (2) preparing phyllanthus emblica fermenting juice; (3) blending and disinfecting; and (4) bottling and packaging. The prepared wine per liter contains 800-1200 thousand international units of SOD. The original anthocyanin in percentage by weight in the wine is 0.01-0.0375%. The wine containing the SOD component prepared by the preparation method not only maintains taste of wine, but also increases various nutritional substances and SOD, and has the edible, beautifying and health-care functions.

The invention discloses stratiomyiid meal-containing pelteobagrus fulvidraco feed and an application thereof. The stratiomyiid meal-containing pelteobagrus fulvidraco feed comprises the following components in parts by weight: 24-40 of fish meal, 6-23 parts of stratiomyiid meal, 27 parts of soybean meal, 22-24 parts of high protein flour, 0.5 parts of fish oil, 0.2-3.4 parts of soybean oil, 0.5 part of choline chloride, 0.4 part of vitamin premix, 0.4 part of mineral premix, 1 part of phagostimulant and 1-2.5 parts of microcrystalline cellulose. The stratiomyiid meal-containing pelteobagrus fulvidraco feed has the advantages that the novel pelteobagrus fulvidraco feed is prepared by replacing part of fish meal with the stratiomyiid meal and can effectively supply all nutrient substances required for pelteobagrus fulvidraco by matching of the stratiomyiid meal and other raw materials, so that the survival rate and weight gain rate of the pelteobagrus fulvidraco can be effectively ensured; the nonspecific immune factor serum lysozyme activity, serum and hepatopancreas SOD (Superoxide Dismutase) active content and leukocyte phagocytic capacity of the pelteobagrus fulvidraco are obviously improved, and the immunity of the pelteobagrus fulvidraco is remarkably improved.

The invention relates to an oral liquid with high SOD (superoxide dismutase) activity and a preparation method of the oral liquid. The method comprises the following steps: juicing edible plant raw materials, performing centrifuging at 1000-4000 rpm for 0-30 min to remove large particles, and performing separation with two ultrafiltration membranes, including a tubular membrane with pore diameterof 60-120 kD for primary separation and a coiled membrane with pore diameter of 8-30 kD for secondary separation; then performing ultra-high pressure treatment at pressure of 200-600 MPa for 1-20 min.The SOD is activated after being treated with the method, the enzyme activity is improved to 10000 U/ml or higher, and the problem of low SOD activity in the product is effectively solved. The product prepared with the method is more beneficial to human health.

The invention discloses a method for preparing longan ferment from a longan kernel. The method comprises the following steps: processing longan, and pasting; performing enzymolysis, sterilizing, fermenting, and spray-drying, thereby finally obtaining the longan ferment which is rich in nutrients. The longan ferment prepared by adopting the method is rich in nutritional substances such as carbohydrate, protein, superoxide dismutase, protease, lactic acid, phenolic substance, lactic acid viable count and the like. The longan ferment can be used as a novel health care product and a cosmetic additive, and thus nutritional and medicinal values of the longan kernel are fully utilized, and the variety of a longan processed product is increased.

The invention discloses an intelligent nanoparticle drug release system capable of delivering biomacromolecules and small organic molecules to cells, a preparation method of the intelligent nanoparticle drug release system, and application of the intelligent nanoparticle drug delivery system in cell delivering of biomacromolecules and small organic molecules (including drugs). By delivering biochemical substances of drug molecules, cell nutrients and cell expellant, physiological metabolism and pathological metabolism are intervened. Molecule delivery of an embodiment of the intelligent nanoparticle drug delivery system includes but is not limited to the following molecules: telomerase cell delivery; antioxidant enzyme (such as SOD (superoxide dismutase) and HPX (hydrogen peroxidase)) cell delivery; cancer suppressor protein (such as p53 and PTEN(phosphatase and tensin homolog deleted on chromosome ten)) cell delivery; and organic chemical and biochemical small molecules (such as atorvastatin-lipitor and coenzyme Q-10) cell delivery. The molecule slow release and control of the nanoparticle drug delivery system has uniqueness and targeting so that the nanoparticle drug delivery system is intelligent.