808 results about "Pectinase" patented technology

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention relates to the field of microorganisms, and discloses an Aspergillus oryzae strain and application thereof.The Aspergillus oryzae can produce protease, aminopeptidase, glutaminase, cellulase, amylase and pectinase at high yield, and when the Aspergillus oryzae is applied to soy sauce brewing, the utilization rate of raw materials can be increased, the content of amino acid in soy sauce can be increased, and the flavor of the soy sauce can be increased.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The preparation method comprises the following steps: S1, crushing cordate houttuynia rhizomes, adding the crushed cordate houttuynia rhizomes into a mixed acid aqueous solution, and carrying out immersion treatment to obtain a cordate houttuynia mixed solution; s2, adjusting the pH value of the herba houttuyniae mixed solution to 4-5, adding pectinase, carrying out primary enzymolysis and enzyme deactivation, adjusting the pH value to 6-7, adding compound enzyme, carrying out secondary enzymolysis and enzyme deactivation, so as to obtain an enzymatic hydrolysate; s3, menthol is added into the enzymatic hydrolysate, water extraction is conducted, and water extract and filter residues are obtained; s4, the filter residues are added into ethyl alcohol, then salicylic acid and diacetyl tartaric acid ester of mono (di) glycerides are added, alcohol extraction and filtering are conducted, and an alcohol extraction solution is obtained; s5, mixing the water extract with the alcohol extract, and performing reduced pressure distillation to obtain the herba houttuyniae extract. According to the method, the extraction rate of polysaccharide and flavone in houttuynia cordata can be effectively increased, and meanwhile, the prepared houttuynia cordata extract has better oxidation resistance and has a good application prospect.

The invention provides an ultrasonic-assisted red pomelo polyphenol extraction method and application. The polyphenol component in the red pomelo is extracted through the following steps: firstly, performing activation pretreatment on red pomelo dry powder by adopting a low-temperature plasma device, and destroying a cell wall structure so as to improve the accessibility of combined polyphenol; then cellulase, pectinase, hemicellulase and beta-glucosidase are added into a biodegradable deep eutectic solvent to synergistically hydrolyze the cell wall polysaccharide, and combined polyphenol is released; then applying pulse type ultrasonic wave to assist extraction under a low-temperature condition, so that polyphenol is fully dissolved out and enters the eutectic solvent; and finally, carrying out solid-liquid separation to obtain the red pomelo polyphenol extract. Compared with a conventional heat extraction method, the method has the advantages that the extraction rate of the polyphenol of the red pomelo is remarkably increased at a lower temperature, the degradation of the polyphenol is effectively avoided, and the content of the total polyphenol and the antioxidant activity are increased; the method is green and environment-friendly, and can be applied to the health industry as an antioxidant.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention belongs to the technical field of biology, and relates to a combined method for improving the taste of cerasus humilis clear juice and increasing the content of available calcium and zinc, which comprises the following operation steps: S1, removing impurities from cerasus humilis fruits, cleaning, denucleating and crushing to obtain cerasus humilis pulp; s2, performing primary enzymolysis on the cerasus humilis fruit pulp to obtain primary enzymolysis cerasus humilis fruit pulp; s3, fermenting the cerasus humilis fruit pulp to obtain fermented cerasus humilis fruit pulp; s4, performing secondary enzymolysis on the fermented cerasus humilis fruit pulp to obtain secondary enzymolysis cerasus humilis fruit pulp; and S5, filtering, sterilizing and filling the secondary enzymolysis cerasus humilis pulp to obtain a cerasus humilis clear juice product. The combined method of high-speed dispersion machine release, pectinase enzymolysis, issatchenkia terricola fermentation and joint enzymolysis of cellulase, beta-glucosidase and alpha-rhamnosidase is utilized, the product taste is effectively improved while the yield of the cerasus humilis clear juice product is increased, and the cerasus humilis clear juice product is palatable in sourness and sweetness, clear, transparent and bright, full in flavor and suitable for large-scale production. And the contents of calcium and zinc are increased by more than 65% and 59%. The product has good calcium and zinc supplementing effects.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention belongs to the technical field of biological fermentation and cosmetics, and particularly relates to a cortex magnoliae officinalis fermented product with oil-controlling and dandruff-removing effects as well as preparation and application of the cortex magnoliae officinalis fermented product. The preparation method comprises the following steps: performing ethanol ultrasonic extraction on officinal magnolia bark powder to obtain an officinal magnolia bark extract; adding mangnolia officinalis, honeysuckle, green tea and pericarpium citri reticulatae powder into pure water, performing enzymolysis treatment by adopting cellulase and pectinase, then adding a carbon source, prebiotics and inorganic salt, performing high-temperature sterilization, performing cooling, then adding a mangnolia officinalis extract, and performing uniform mixing so as to obtain a fermentation culture medium; the method comprises the following steps: inoculating an aspergillus seed solution and a saccharomyces cerevisiae seed solution into a fermentation culture medium for first-step fermentation, heating and inactivating, cooling, inoculating fruit wine yeast for second-step fermentation, and sterilizing at high temperature, cooling and filtering after fermentation to obtain filtrate which is the cortex magnoliae officinalis fermented product with the effects of controlling oil and removing dandruff. According to the preparation method, enzymolysis and fermentation technologies are combined, and the honeysuckle, the green tea and the pericarpium citri reticulatae are fermented together, so that the antibacterial, anti-dandruff and oil-control effects of the mangnolia officinalis can be synergistically improved.

The invention relates to the technical field of deep processing of paeonia ostii, in particular to paeonia ostii peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis as well as a preparation method and application of the paeonia ostii peptide. The paeonia ostii flower peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis is prepared through water bath, homogenization, alkali extraction and acid precipitation pretreatment, three times of enzymolysis through compound enzyme of acid protease, cellulase and pectinase and alkaline protease and solid-liquid separation, operation is easy, the requirement for equipment is low, and the paeonia ostii flower peptide is suitable for industrial large-scale production. The prepared paeonia ostii flower peptide has good inhibition capability on tyrosinase, hyaluronidase and elastinase, and also has excellent anti-saccharification and anti-inflammatory effects. Besides, five paeonia ostii active peptide fragments are screened out, are high in activity and non-toxic, can be combined with tyrosinase, hyaluronidase, MMP-1, MMP-3 and COX-2 receptor protein, and have the effects of whitening, moisturizing, tightening and promoting collagen synthesis.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention relates to the technical field of breeding feed, and discloses an efficient feed additive based on emblic leafflower residue component optimization and a preparation method thereof. Comprising the following components in parts by weight: 30-60 parts of emblic leafflower residues, 10-20 parts of amino acids, 5-15 parts of a vitamin premix, 3-8 parts of a mineral premix, 5-20 parts of probiotics, 0.6-4 parts of cellulase, 0.45-3 parts of pectinase and 0.75-4 parts of amylase. By adding the emblic leafflower residues, the nutrition of the feed additive is enriched, the emblic leafflower residues are rich in cellulose, polyphenol, flavone and other components, have an antioxidant effect, can help animals to remove free radicals in bodies, enhance the antioxidant capacity of the bodies and improve the immunity, meanwhile, the emblic leafflower residues are wide in source and relatively low in cost, and as a feed additive component, the feed cost is reduced; the resource utilization rate and culture benefits are improved.

The invention relates to the technical field of illicium verum wine production, in particular to fermented illicium verum wine and a preparation process thereof. Comprising the following components in parts by weight: 60-80 parts of water, 5-10 parts of acidified star anise, 0.5-2 parts of a balm extract, 0.1-0.5 part of sulfurous acid, 0.1-0.5 part of activated pectinase, 0.5-2 parts of active dry yeast, 1-3 parts of a thiamine nutritional agent, 10-20 parts of white granulated sugar, 0.5-2 parts of bentonite GB and 0.1-0.5 part of casein. In the design, after the star anise is acidified, the taste of the wine can be improved, the wine is mellower and softer, and the complexity and layering sense of aroma can be increased; carbonic anhydrase mainly acts on generation and decomposition of carbonate ions, so that the risk of acid residue can be reduced, and other beneficial components or flavor substances in illicium verum are not interfered; the melissa officinalis extract is rich in various volatile aromatic compounds, so that the wine body can be endowed with unique elegant fragrance and complex layering sense, and the overall flavor experience is enhanced.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention discloses a preparation method of a whole-bean rumex hanus nutrient plant enzyme, which comprises the following steps: S1, taking soybeans, and preparing whole-bean minced soybean; taking rumex hanus to prepare rumex hanus whole pulp; taking grains, and preparing whole grain pulp paste; taking fruits and vegetables, and preparing fruit and vegetable puree; s2, mixing the whole-bean minced soybean, the rumex hanus whole pulp, the whole-grain minced pulp and the fruit and vegetable primary pulp obtained in the step S1 to obtain whole-bean rumex hanus nutrient plant enzyme primary pulp, and then sequentially performing enzymolysis by combining cellulase with pectinase, amylase, saccharifying enzyme and protease; and S3, taking the whole-bean rumex hanus nutrient plant enzyme primary pulp subjected to enzymolysis in the step S2, performing anaerobic fermentation by using saccharomycetes and / or distiller's yeast under the negative pressure condition of-0.1 to-0.06 MPa, and filtering to obtain the whole-bean rumex hanus nutrient plant enzyme. The invention further provides the whole-bean rumex hanus nutrient plant enzyme. According to the technical scheme, the defects of traditional bean products, rumex hanus food and edible plant enzymes can be overcome.

The invention relates to the field of extraction of sacha inchi oil, in particular to a preparation method and application of sacha inchi oil, and aims to solve the problems of large loss of heat-sensitive components, solvent residue and low oil yield in the traditional process. The ratio of cellulase to pectinase to xylanase to lipase is 3: 2: 1: 0.5, and carrying out enzymolysis for 2-3 hours at the pH value of 4.5-5.5 and the temperature of 45-50 DEG C; the obtained enzymolysis product is subjected to 28-40kHz ultrasonic treatment for 20-30 minutes, and then is subjected to two-stage gradient squeezing (pre-squeezing at 50-55 DEG C to final squeezing at 35-40 DEG C) in combination with 0.1 mu m ceramic membrane filtration, so that efficient oil extraction and active ingredient protection are realized. The oil yield of the sacha inchi oil is increased to 50.0%, the content of alpha-linolenic acid is larger than or equal to 42%, the content of vitamin E reaches 158 mg / 100 g, no solvent is left, and the sacha inchi oil is suitable for the fields of functional food and cosmetics.

The invention discloses a preparation method of water-soluble white tomato freeze-dried powder, which comprises the following steps: S1, pretreating fresh white tomatoes, and then crushing and pulping to obtain white tomato pulp; s2, adding cellulase and pectinase into the white tomato pulp obtained in the step S1, and then performing low-temperature enzymolysis treatment to obtain white tomato enzymatic hydrolysate; s3, the white tomato enzymatic hydrolysate obtained in the step S2 is filtered to remove residues, and white tomato enzymatic hydrolysate filtrate is obtained; and S4, adding soluble plant fibers into the white tomato enzymolysis filtrate obtained in the step S3, performing low-temperature mixing, performing low-temperature sterilization treatment, and then performing freeze-drying to prepare powder, thereby obtaining the water-soluble white tomato freeze-dried powder. The process is relatively simple, nutritional ingredients of the white tomatoes can be completely reserved, and the prepared freeze-dried powder is excellent in water solubility and can be applied to the fields of oral liquid, cosmetics and the like.

The invention belongs to the technical field of preparation of food additives, and particularly relates to a method for extracting sa-hao seed gum and oligosaccharide thereof by using a compound enzyme method. According to the method provided by the invention, complete artemisia desertorum seeds are taken as a raw material, enzymolysis is carried out by using a compound system consisting of xylanase, cellulase and pectinase, and after enzyme deactivation and alcohol precipitation, the artemisia desertorum gum or the artemisia desertorum gum oligosaccharide can be obtained. Compared with a traditional water extraction method and an acid-base extraction method, the extraction yield is remarkably increased through the enzymolysis synergistic effect, the process is green and safe, no chemical residue exists, the molecular weight of the product is controllable, and high purity and functional activity are both achieved. The method is easy and convenient to operate, low in cost and suitable for large-scale production, and has wide application prospects in the fields of food, medicine and functional oligosaccharide development.

The present application belongs to the technical field of biological fermentation, and discloses a natural plant fermentation liquor, which is prepared from acanthopanax, angelica sinensis, rosa davurica, phellinus baumii, tea leaves, water, mixed enzymes and mixed bacterial agents; wherein the mixed enzymes include cellulase, pectinase and tannase, and the mixed bacterial agents include lactobacillus plantarum, schizosaccharomyces and lactobacillus acidophilus.The natural plant fermentation liquor has high safety, good clarity after adjusting the concentration of crude drugs, and can be added into a cosmetic matrix.The preparation method is simple, and the effective active ingredients of the natural plants and tea leaves are extracted by the synergistic effect of bacteria and enzymes to the maximum extent.In addition, the present application also discloses the application of the natural plant fermentation liquor in preparing cosmetics for treating oily sensitive skin.

The present invention relates to a prebiotic feed additive for promoting intestinal health of pigs, belonging to the technical field of feed, the prebiotic feed additive of the present invention comprises prebiotic components and biological adjuvants, the prebiotic components comprise oligofructose, oligoisomaltose, inulin and resistant dextrin, the biological adjuvant is a product obtained by co-enzymatic hydrolysis of dandelion and houttuynia cordata by hemicellulase and pectinase, then fermented by Lactobacillus acidophilus and Propionibacterium freudenreichii in sequence, and finally enzymatic hydrolyzed by bromelain and trypsin step by step. The components in the additive are used in combination to have a good effect of promoting intestinal health of pigs, can balance and improve the intestinal microenvironment, improve feed nutrient utilization, enhance immunity, reduce diarrheal diseases, and promote growth and weight gain.

The invention relates to an enzyme and bacterium combined feed additive for pre-digestion of feed raw materials as well as preparation and application of the enzyme and bacterium combined feed additive. The feed additive comprises: (a) an enzyme preparation, which is composed of protease, cellulase, xylanase, mannase, phytase, pectinase and keratinase, and has a total enzyme activity of 42500-45000 U / g; the probiotics are composed of bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae, and the viable count is larger than or equal to 107 CFU / g; and (c) the mass ratio of enzyme to bacteria is (1-2): 1. According to the additive, the enzyme preparation and the probiotics are jointly used, and the raw materials are effectively pretreated, so that the nutritional value and the digestibility of the feed are improved. The feed additive disclosed by the invention comprises a specific enzyme preparation and probiotic combination, and predigestion treatment on the feed raw materials is realized through an optimized preparation process and fermentation process, so that the nutritional value and the utilization value of the feed raw materials are improved. The additive is simple in preparation method, low in cost and environment-friendly, and has a wide application prospect.

The invention belongs to the technical field of food packaging and fresh-keeping materials, and discloses an ethylene adsorption and pH / pectinase double-response intelligent antibacterial composite film as well as a preparation method and application thereof. The composite film comprises a base film and a functional carrier distributed in the base film, the functional carrier is a material ZIF-8-cinnamyl aldehyde-pectin capable of adsorbing ethylene and responding to pH and pectin, and the base film comprises a film-forming polymer and a natural antibacterial agent. According to the composite film, efficient adsorption of ethylene released in the storage period of fruits and vegetables is achieved through a porous structure of ZIF-8, meanwhile, a synergistic effect is formed by an acid environment naturally generated in the fruit and vegetable ripening and rotting process and pectinase, and intelligent slow release of cinnamyl aldehyde loaded on ZIF-8 is accurately triggered; cinnamyl aldehyde can form a double-channel intelligent antibacterial system with the natural antibacterial property of chitosan in a film base material and the metal ion antibacterial property of ZIF-8, maturation and aging of typical respiratory climacteric fruits and vegetables can be remarkably delayed, and the antibacterial film has extremely high industrial application potential.

The invention relates to a probiotic fermented fruit and vegetable juice beverage and a preparation method thereof, and belongs to the technical field of food. The invention provides a preparation method of a probiotic fermented fruit and vegetable juice beverage, which mainly comprises the following steps: mixing fruit and vegetable raw materials with deionized water, homogenizing at high pressure, adding polydextrose and resistant starch, stirring for dissolving, sieving, adjusting the pH value, adding pectinase and cellulase, performing enzymolysis, inoculating compound bacteria into enzymatic hydrolysate, performing two-stage fermentation, and obtaining the probiotic fermented fruit and vegetable juice beverage. And adding a fructus momordicae extract into the obtained fermentation liquor, sieving, carrying out pasteurization, immediately cooling to 4 DEG C, filtering, and carrying out sterile filling, thereby obtaining the fructus momordicae health-care wine. The apple, the kale, the jerusalem artichoke, the mandarin orange, the momordica grosvenori extract and the like are compounded to provide multiple trace element supplementation for a human body, lactic acid and short-chain fatty acid are generated in the synergistic double-bacterium fermentation process, and intestinal tract movement is effectively promoted.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses a method and a system for producing high-purity medium-chain fatty acid (MCFA) based on waste molasses two-stage regulation fermentation. Comprising the following steps: (1) diluting waste molasses, then adding a compound enzyme agent of pectinase, beta-glucanase and cellulase, and coupling microwave to assist treatment; (2) inoculating the pretreated waste molasses with a composite flora containing a clostridium kluyveri mutant strain and a Megasphaera elsdeni engineering bacterium, and carrying out a fermentation treatment on the waste molasses, wherein the composite flora contains the clostridium kluyveri mutant strain and the Megasphaera elsdeni engineering bacterium; (3) sequentially performing two-stage dynamic regulation and control fermentation on the waste molasses inoculated with the composite flora through a pH-stat control strategy to realize efficient conversion from butyric acid to hexanoic acid / octanoic acid; and (4) a hydrophobic hollow fiber membrane module is adopted to realize on-line removal of the product, and the inhibition effect of the product is relieved. According to the technology, the yield of the MCFA produced from the waste molasses reaches 0.45 g / g COD, the cost is reduced by 68% compared with that of a traditional substrate, and a new way is provided for recycling of sugar manufacturing waste.

The invention discloses a passion fruit juice clarification process which comprises the following steps: S1, pectinase treatment: adding pectinase into passion fruit juice, and stirring and reacting for 24-72 hours under the conditions that the temperature is 20-30 DEG C and the pH value is 3.5-4.5; the addition amount of the pectinase is 0.5-1.5% of the mass of the passion fruit juice; s2, bentonite treatment: adding bentonite into the fruit juice treated in the step S1, and standing and adsorbing for 24-48 hours under the condition that the temperature is 25-35 DEG C; the addition amount of the bentonite is 0.1-0.5% of the mass of the passion fruit juice; and S3, performing centrifugal treatment: putting the fruit juice treated in the step S2 into a centrifugal machine, performing centrifugal treatment for 6-12min under the conditions that the temperature is 4-6 DEG C and the rotating speed is 3500-4500r / min, and collecting supernate, thereby obtaining the clear passion fruit juice. By accurately regulating and controlling the combination ratio and process parameters of the clarifying agent, the turbid substances in the passion fruit juice can be efficiently removed.

The invention relates to a preparation method and application of an environment-friendly silicate scale pipeline cleaning treatment agent, and discloses preparation and application of a silicate scale cleaning treatment agent which is used in a water treatment process and is formed in oil-water wells, strata, ground gathering and transportation treatment pipelines and various devices in petroleum and petrochemical industries in the fields of industrial water treatment and petrochemical engineering. The cleaning treatment agent comprises the following components: edible radishes, pectinase, methanol, an acid-base regulator, a penetrating agent, a dispersing agent and water. The scale remover disclosed by the invention is nearly neutral and can be used for rapidly dissolving silicate scale; the biodegradability is realized, and the environmental pollution is extremely low; the treatment cost of a post-treatment agent can be effectively reduced. Edible radishes are adopted as raw materials, and the green and environment-friendly pipeline cleaning treatment agent capable of rapidly dissolving silicate scales is prepared. The scale inhibitor is applied to multiple fields of industrial and civil water treatment, petroleum and petrochemical engineering, mechano-electronics and the like, and insoluble silicate scales in pipelines and equipment can be rapidly dissolved.

The invention discloses a pH and pectinase dual-response type pesticide microcapsule and a preparation method and application thereof.The pH and pectinase dual-response type pesticide microcapsule is of a core-shell structure, the core structure is prochloraz ionic liquid, and the shell structure sequentially comprises a metal-phenol network layer and a pectin layer from inside to outside; and the metal-phenol network layer is a tannic acid-copper network layer. In order to further strengthen long-term autonomous management of plant diseases and reduce the problem of pesticide leakage in a traditional spraying method to the maximum extent, the invention further provides an adhesive gel patch, agar hydrogel is used for stabilizing pesticide microcapsules, silicone rubber is used as a protective layer, and stable attachment of the patch to leaves is achieved by means of magnets. The preparation process of the pH and pectinase double-response type pesticide microcapsule and the adhesive gel patch thereof has the advantages of rapidness, economy, environmental protection and suitability for industrial production, and has huge potential in sustainable management of plant diseases and precision agriculture.