4930 results about "Pectinase" patented technology

The invention disclosed relates to a method for the extraction of sweet glycosides from the Stevia rebaudiana Bertoni plant and recovery of individual rebaudioside A and stevioside. The extraction is developed in the presence of pectinase, and the extract is purified using cyclodextrin and bentonite. High purity rebaudioside A is obtained by crystallization and recrystallization from ethanol. High purity stevioside is prepared from the filtrate by purification with cyclodextrin, bentonit, and ion exchange resins. The enzymatic modification of the rebaudioside A, stevioside and the purified extract is carried out using the transferring enzymes derived from Thermoactinomyces vilgaris and Bacillus halophilus.

The invention disclosed relates to a method for the extraction of sweet glycosides from the Stevia rebaudiana Bertoni plant and recovery of individual rebaudioside A and stevioside. The extraction is developed in the presence of pectinase, and the extract is purified using cyclodextrin and bentonite. High purity rebaudioside A is obtained by crystallization and recrystallization from ethanol. High purity stevioside is prepared from the filtrate by purification with cyclodextrin, bentonit, and ion exchange resins. The enzymatic modification of the rebaudioside A, stevioside and the purified extract is carried out using the transferring enzymes derived from Thermoactinomyces vulgaris and Bacillus halophilus.

Enhanced removal and / or control of adhesives or sticky materials, “stickies”, from recovered paper stock or virgin pulp fibers is achieved using a combination of enzyme treatment with adsorbents and / or absorbents. Pulp stock to be treated is typically obtained from old magazines, newspapers, household waste, but may include corrugated boxes and office waste. Virgin pulps may include mechanical, chemical, or semi-chemical pulps. Enzymes typically include hydrolases such as cellulases, hemicellulases, pectinases, amylases, and lipases such as esterases, lyases such as pectate lyases, and oxidoreductases. Adsorbents include activated bentonite, microparticles, talc, clay and modified silica. Absorbents typically include water soluble polymers, dispersants, coagulatants and agglomerants.

The invention relates to a fluorescent pseudomonad DA4 strain as well as an acquisition method and application thereof. The fluorescent pseudomonad DA4 strain has pectase and hemicellulase producing capacity and flax degumming activity. The fluorescent pseudomonad strain screened by the invention has fast propagation, high antipollution capacity and good heat and alkali resistance; since a composite degumming enzyme solution is used for flax degumming, the degumming period is shortened greatly, and the system and the culturing process is safe for operation without toxicity or environmental pollution; the invention can shorten the flax degumming period, enhance the flax yield and the strength of flax fibers, improve the quality of the flax fibers by adopting the composite degumming enzyme solution for flax microbial degumming, thereby being beneficial to popularization; in addition, the invention can not only be used for flax raw stem degumming, roving scouring and the biological pretreatment of flax fabrics, but also can be used for the degumming and the biological pretreatment of hemp bast fibers, i.e. ramie, jute, hemps, and the like.

The present invention relates to a passiflora coerulea and honey peach complex juice and a preparation method thereof. The complex juice consists of 20-30% passiflora coerulea juice, 10-15% honey peach juice, 3-5% honey and water and the preparation method of the complex juice is made of the following steps: washing, cleaning and cutting the passiflora coerulea, removing the fruit pulp with seeds to squeeze juice, washing and cleaning honey peaches, peeling honey peaches to squeeze juice, mixing the two juices evenly and heating to a temperature of 20-60 DEG C, adding pectinase and amylase to conduct enzymolysis, filtering the juice and adding water and honey to flavor, removing parts of bacteria with sterilizing filtration membranes; then homogenizing and degassing the sterilized juice, and vacuum packaging and treating the packaged juice with super-high pressure sterilization and enzyme inactivation treatment, thereby obtaining the complex juice. The preparation method of the complex juice can maintain the unique flavor of passiflora coerulea and delicate fragrance of honey peaches, thus the prepared complex juice has authentic taste of the raw juice, natural and lingering flavor, and improved the quality of fruit juice, and meet the pursuit of natural fruit juice of the majority of consumers.

The invention discloses a complex enzyme preparation for feeding piglets. The complex enzyme preparation for feeding piglets comprises the following seven enzymes: acid protease, amylase, xylanase, beta-glucanase, cellulase, pectinase and phytase; and the enzymatic activity ratio of the seven enzymes is 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02). By applying the complex enzyme preparation disclosed by the invention, various anti-nutritional factors in feed can be degraded, the viscosity of chyme in an intestinal tract can be reduced, and the metabolic energy of the feed can be improved; the protein and starch digestibility of the piglets can be improved; excessive reproduction of harmful microorganisms in the intestinal tract can be reduced, damage to the intestinal wall can be reduced, and the microecological balance of the intestinal tract can be regulated; the survival rate and disease resistance of the piglets can be improved, the diarrhea rate can be reduced, and the overall uniformity is improved; and the weight gaining of the piglets can be promoted, and the cultivation cost is reduced.

The invention belongs to the technical field of feed additives, and in particular relates to complex enzyme for a feed. The complex enzyme comprises 7,500 to 15,000U / g of xylanase, 1,000 to 3,000U / g of mannose, 50 to 80U / g of galactosidase, 200 to 700U / g of cellulose, 500 to 1,500U / g of beta-glucanase, 150 to 1,000U / g of alpha-amylase, 2,000 to 10,000U / g of protease and 100 to 200U / g of pectinase. By adding corresponding enzyme preparations, the anti-nutrition function of the complex enzyme is reduced, the feed utilization is improved, and the animal production efficiency is improved, so the problems that the utilization rate of animal feed raw materials is low and the animal production performance is inhibited are effectively solved; meanwhile, the complex enzyme has the advantages of reasonable raw material mixing, good effect, and low overall cost.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention relates to a natural tea essence prepared by using tea and a preparation method thereof. The tea is taken as a raw material; high-purity natural tea essences with different tea aroma characteristics are prepared through compound enzymolysis, releasing, steam distillation under reduced pressure, cold trap recycling, low temperature freezing concentration, centrifugal oil-water separation, and trace moisture adsorption by using anhydrous sodium sulfate; and the compound enzymolysis and the releasing are realized by adding pectinase, cellulase or pectinase and cellulose complex enzyme, and beta-glucosidase. In the preparation method, a pectinase and cellulose complex enzyme method is adopted for enzymolysis to facilitate cracking cell walls of the tea so as to facilitate liberation and separation of aroma components from the tea, and improve the yield of the essence; therefore, the method is a method for industrially producing the natural tea essence which is energy-saving, low in cost, environmentally friendly and high in efficiency.

A bio-enzyme consists of pectinase, protease, lipase, cellulose, catalase and amylase; the application of bio-enzyme catalysis in residual sludge treatment is that: the residual sludge is treated by utilizing the high-efficiency biocatalysis of the bio-enzyme, odor molecules in the sludge are decomposed and deodorized through enzyme technology, pathogenic bacteria, ova and larvae are killed by utilizing an acid environment formed by inorganic acid generated by the sludge and a high temperature environment in the fermentation process, heavy metal ions in the sludge are stabilized by utilizing the chelation of the bio-enzyme, and the sludge is catalyzed by the enzyme, so that the sludge and water are quickly separated, a large amount of supernate is discharged, the sludge volume is reduced, and the sludge in inland river is reduced and is subjected to innocent treatment; meanwhile, through the bio-enzyme catalysis, long-chain molecules in the sludge are converted into short-chain molecules, polysaccharide and cellulose are converted into protein and amino acid, nutritional components of organic fertilizers are remained, the fertilizer efficiency is improved and the fermentation period can be shortened.

The invention discloses a laying hen composite premix, and belongs to the technical field of feed composition. A composite enzyme preparation containing beta-mannase, beta-glucanase, acidic proteinase, phytase, pectinase, and amylase is adopted to eliminate the anti-nutrient factors in soybean meal. Yeast cell wall type mycotoxin absorbent is used to effectively eliminate the hazardous factors that may affect the intestine health and are caused by using a large amount of corns. A stomach fortifying acid is used to effectively adjust the pH value of intestines. The used wood chips in the premix are soft and high in fiber content, do not hurt the intestines, do not pollute the environment, can effectively strengthen the digesting and absorbing abilities of laying hens, and have a stronger bearing and water-absorbing performance. The materials mentioned above are systematically and scientifically used to guarantee that the intestines of the laying hens be in the best state. The premix can improve the immunity and disease resistant performance of laying hens, and makes the laying hens exert the maximal laying ability. Moreover, the water content of manure is reduced, and the economic profit of farms that use the premix is prominently improved.

The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention relates to a multienzyme microorganism and plant growth promotion additive. An active regulator containing multienzyme microorganism and leavening, amylase, protease, cellulose, pectase and polyase are used as base stocks, are added with chitin, various elements and vitamins in micro quantity, amino acid and are added with a microorganism and leavening inoculum Em bacterial solution, are mixed and are fermented to prepare the multienzyme microorganism and plant growth promotion additive. The additive can effectively improve soil, strength land capacity, reduce plant diseases and insect pests, fully excavate the soil potential, bring organic nutritive compositions for plants, promote the healthy and rapid growth of the plants, remove negative influence of pesticide and fertilizer, protect soil and entironment, is beneficial for society and people, adopt waste utilization, and has low investment, low cost, rapid effect and good effect.

The invention discloses a preparation method of a mushroom ferment beverage with effect of improving immunity, and belongs to the technical field of food beverages. According to the method disclosed by the invention, mushrooms and / or medicinal plants, fruit and ginseng are adopted as raw materials. The method comprises the following steps: slicing, chopping or crushing the raw materials; uniformly mixing the sliced, chopped or crushed raw materials so as to obtain a mixture; after adding sugar in the mixture, fermenting the mixture in ferment microorganisms; adding sugar when the sugar content is lowered; continuing fermentation so as to obtain the mushroom ferment beverage. According to the method disclosed by the invention, ferment bacteria can regulate intestinal flora and generate SOD (superoxide dismutase), so that the raw materials can be successfully fermented; the prepared ferment beverage contains protease, lipase, SOD, pectinase, lucid ganoderma, cauliflower fungus, pleurotus eryngii polysaccharide, rare ginsenoside, Rb2, C-K and the like. According to the method disclosed by the invention, the fermentation liquor and mycelium of the mushrooms can be directly used for fermentation; compared with sporophore, the raw materials are easier to obtain, and the method has the advantages of being short in fermentation cycle, easy in formula, simple in fermentation process, and the like.

The invention relates to the technical field of straw degradation, in particular to a straw degradation actinomycete and application thereof. The straw degradation actinomycete belongs to streptomyces griseorubens (Streptomyces griseorubens), and the preservation serial number is CGMCC N0.5706. Compared with the prior art, the straw degradation actinomycete and the application have the advantagesthat the streptomyces griseorubens has strong capacity for producing cellulose, hemicellulase, pectinase and ligninase, is free of negative effects on carbon cycling of the nature, and is free of damage to the environment; the streptomyces griseorubens has strong straw degradation capability, enables straw weightlessness rate to achieve 76% after staying in a straw degradation culture medium for 30 days, and is low in culture cost, convenient to operate and easy to industrially apply; and the streptomyces griseorubens has certain antagonism on plant disease microorganism in the soil.

The invention mainly discloses a cotton type woven fabric continuous biochemical pretreatment technology, relating to the technical field of composite preparation of a composite biological enzyme preparation and a composite pretreatment preparation and the application of the composite preparation in the cotton type woven fabric pretreatment technology. The technology comprises the steps of: usingthe composite biological enzyme preparation (the preparation comprises amylase, pectinase, neutral cellulose enzyme, penetrating agent JFC and stabilizing agent) to pretreat the woven fabric, therebypartially or wholly removing starch size, pectin and the other natural or artificial impurities on the woven fabric, and improving the wettability of the woven fabric; and water washing and padding the composite pretreatment liquid (the liquid comprises composite pretreatment agent, hydrogen peroxide and refined penetrating agent); and water washing with steam, wherein the whiteness, the capillary effect, the size removing rate and the strength of a product are better than the normal technology. The technology also solves the problems of high sewage alkalinity, large energy consumption and bad product quality, and the like, in a normal pretreatment technology which takes caustic soda as a main raw material.

The invention belongs to the technical field of environmentally water treatment and particularly relates to a water purifier for treating production wastewater of a rubber accelerator NOBS and a wastewater treatment method. The water purifier comprises a flocculating water purifier, a microbial water purifier and an enzyme preparation, wherein the flocculating water purifier consists of polyaluminum ferric chloride, polyacrylamide, ferrous sulfate and aluminum sulfate; the microbial water purifier consists of nitrobacteria powder, paracoccus denitrificans powder, thiobacteria powder, aniline degradation bacteria powder, flocculating bacteria powder, bacillus siliceous powder and bacillus subtilis powder; the enzyme preparation consists of pectinase, cellulose, oxidordeuctase, xylanase, alkaline protease and monoamine oxidase. The water purifier for treating sewage provided by the invention is good in water purification effect, quick in water purification speed, high in safety and free of secondary pollution. By using a material with a desorption capacity as a raw material, sewage is treated by being assisted by microorganisms, so that the water purifier is wide in application range, and the treated water is extremely high in transparency and can be recycled.

The invention discloses a method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine, belonging to the field of food nutrition and the technical fields of microorganisms and feed. The method comprises the following steps: extracting Chinese herbal medicine by adopting asynchronous fermentation and enzymolysis technologies, namely adding water and nutritive salt in proportion to prepare a Chinese herbal medicine fermentation liquid after a plurality of Chinese herbal medicines are subjected to impurity removal and super-micro smashing; firstly inoculating Aspergillus niger for aerobic fermentation, thus cellulose and pectinase are prepared; and then inoculating enterococcus faecium and lactobacillus plantarum for anaerobic fermentation; and then carrying out enzymolysis on damage cell walls, and dissolving out active substances of saponin, alkaloid, phytosterol, enzymic preparations, organic acid and the like. A plate-and-frame filter press is adopted to filter the fermentation liquid, the filtration liquid is directly subjected to low-temperature spray drying to prepare antibiotic substituent powder A; a filtration cake is dried by adopting a roller drier to prepare antibiotic substituent powder B, and the power A and the powder B are mixed and formulated in proportion to replace fermented enzymolysis Chinese herbal medicine extracts of antibiotic for feed.

The invention discloses a method for brewing dendrobium nobile wine. Dendrobium nobile is used as one of raw materials, corns and potatoes are used as matrixes, and the dendrobium nobile wine is prepared by the procedures of pretreating, mixing, medicine feeding, primary fermenting, secondary fermenting, squeezing, clarifying, wine decocting, inspecting, bottling and storing. During production, the dendrobium nobile serving as the raw material is subjected to combined hydrolysis by using cellulose and pectinase, so that nutrients beneficial to a human body, contained in the Dendrobium nobile,can be separated out fully; and meanwhile, the corns and the potatoes are used as the matrixes, so that the production cost is reduced and simultaneously the prepared dendrobium nobile wine is red brown, has a unique scorch flavor, is mellow, palatable and slightly bitter and has a long-lasting aftertaste. A product of the dendrobium nobile wine has multiple obvious health-care functions of removing lead, enhancing memory ability and immunity, deferring senility, increasing anoxia resistance, preventing osteoporosis and the like. The method is easy to master and can be applied to scale production.

The invention provides a method that takes fresh tea leaves as raw materials to process instant tea powder, which can prevent loss and deterioration of nutrition substance when in the tea manufacturing process. The technique is that: when the raw material is extracted, pectinase is added to enhance extraction rate of the tea leaves and to extract solid substance from solution after the processes of extracting, enzymatic hydrolysis, filtration, condensation and drying, etc. of the raw materials; then tannase is added for enzymatic hydrolysis, so as to resolve the deposition problem of the tea soup and to reduce the bitterness of the instant tea powder and improve the dissolution ability of the tea powder in cold water. The technologies of membrane separation and condensation are adopted in filtration and condensation process; and spray drying or vacuum freeze drying is adopted in drying process; therefore, the whole production process is carried out at relatively low temperature, which maximally preserves the nutrition substances and flavor substances, and the produced instant tea powder is abundant in nutrition and has fresh and soft taste.

The invention discloses a method for producing table vinegar by fermenting yellow serofluid and bean dregs, which comprises the following steps: dehydrating bean dreg byproducts from bean product processing, and performing puffing pretreatment; sterilizing or boiling yellow serofluid at a high temperature, cooling, adding the treated bean dregs and a cellulase / pectinase composite enzyme preparation, and performing enzymolysis for 24 hours; and adding bean milk and 95% edible alcohol into the enzymolysis solution, inoculating with a Saccharomyces cerevisiae seed solution and an acetic acid bacterium Huniang A.S1.01 seed solution, performing aerobic fermentation at 20-30 DEG C for 5-10 days, centrifuging the matured fermentation solution, filtering, and sterilizing to obtain the finished table vinegar product. The method is low in cost, high in return, short in production cycle, high in equipment utilization rate and huge in market potential, avoids using grain materials, can relieve argument and scruple of all levels in the society about safety of table vinegar (industrial glacial acetic acid blending phenomenon), and saves grains.

The invention discloses an alkaline pectinase production method and application in papermaking pulping. The alkaline pectinase production method comprises the steps of: selective breeding of a bacterial strain, culturing of the bacterial strain, fermentation through shaking a flask, and preparation of an enzyme liquid. The method is characterized in that the bacterial strain subjected to the selective breeding is achieved through separated screening of a naturally acquired bacterial sample and mutagenesis of the bacterial strain at cellular level, i.e. heat-resistant Bacillussubtillis with a laboratory number of MAPLE61 and preservation number of CCTCC NO:M2010004; and when the heat-resistant Bacillussubtillis is used for papermaking pulping, raw materials are soaked with an alkaline pectinase solution after the pickling treatment. When the invention is used for papermaking pulping, the prepared chemical pulp has favorable strength property and bleaching property; and after simple oxidation chlorine bleaching, the whiteness of the pump can reach 80-90 percent, breaking length can reach 6000-7000m, and meanwhile, alkali consumption is reduced about 20-40 percent and the production cost is reduced about 20 percent.

The invention discloses a complex enzyme preparation for a growing and fattening pig feed. The complex enzyme preparation for the growing and fattening pig feed comprises the following eight enzymes: acid protease, amylase, beta-mannase, xylanase, beta-dextranase, cellulase, pectinase and phytase; and the enzyme activity ratio of the eight enzymes is sequentially 1: (0.8-14.5): (0.19-0.3): (33.7-35): (25-30): (3.2-3.5): (0.19-2.0): (0-0.025). The complex enzyme preparation can degrade various anti-nutritional factors in the feed, reduce the chime viscosity of the intestinal tract, improve the metabolic energy of the feed, improve the digestion rate of growing and fattening pigs on protein and starch, reduce the nutrient content of the chime entering the intestinal tract, effectively inhibit the reproduction of harmful microbes in the intestinal tract, reconcile the micro-ecological balance of the intestinal tract, promote the health of the pigs, improve the average daily gain of the growing and fattening pigs, reduce the feed-meat ratio, shorten the maintenance period and improve the economic benefit.

The invention discloses a preparation method of yak bone collagen peptide. The method is characterized by comprising the following technological processes: pretreatment of raw materials, gelatin extraction, separation of a collagen extracting solution, composite enzymatic hydrolysis, enzyme deactivation and sterilization, separation of a peptide solution, decoloring and deodorizing, concentrating and drying. The compound enzyme for composite enzymatic hydrolysis is prepared from protease, amylase, pectinase, cellulose and xylanase. The method has the advantages that the gelatin extraction process is simple, oil and gelatin are completely separated by using flow of a mixed solution, the enzymatic hydrolysis temperature is 48-52 DEG C, the time is 4-6 hours, the temperature is reduced in comparison with common enzymatic hydrolysis temperature, the preparation time of the product is shortened, and the method comprises enzyme deactivation and sterilization procedures, and decoloring and deodorizing procedures, so that the product is good in taste, and applicable to preparation of the yak bone collagen peptide.

A concentrated sweet potato juice as a functional food with unique taste is prepared from sweet potato through removing peel, adding water, breaking to become jam, squeezing to obtain juice, regulating pH=4-6, hydrolyzing by beta-amylase, decomposing by pectinase, ultrafiltering, concentrating and loading in cans.

The invention relates to a method for extracting chlorogenic acid from honeysuckle by using a compound enzyme method and researches a novel process for extracting a main medicinal effective component of the chlorogenic acid from the honeysuckle by a cellulose and pectinase compounding enzyme method. Through a single factor experiment and an orthogonal test, optimal process conditions for extracting the chlorogenic acid from the honeysuckle by using the compound enzyme method can be optimized: the enzymolysis temperature is 45DEG C, the pH is 4.5, the compound ratio of the cellulose to the pectinase is 1.0:0.3 and the enzymolysis time is 1.5 hours. The test result shows that the process repeatability is favorable and the result is stable. The extraction rate of the chlorogenic acid extracted from the honeysuckle by using the compound enzyme method can reach 83.06 percent which is improved by 16.93 percent in comparison with the extraction rate of the chlorogenic acid extracted by using a water extraction process.