4913 results about "Pectinase" patented technology

The invention disclosed relates to a method for the extraction of sweet glycosides from the Stevia rebaudiana Bertoni plant and recovery of individual rebaudioside A and stevioside. The extraction is developed in the presence of pectinase, and the extract is purified using cyclodextrin and bentonite. High purity rebaudioside A is obtained by crystallization and recrystallization from ethanol. High purity stevioside is prepared from the filtrate by purification with cyclodextrin, bentonit, and ion exchange resins. The enzymatic modification of the rebaudioside A, stevioside and the purified extract is carried out using the transferring enzymes derived from Thermoactinomyces vilgaris and Bacillus halophilus.

Enhanced removal and/or control of adhesives or sticky materials, “stickies”, from recovered paper stock or virgin pulp fibers is achieved using a combination of enzyme treatment with adsorbents and/or absorbents. Pulp stock to be treated is typically obtained from old magazines, newspapers, household waste, but may include corrugated boxes and office waste. Virgin pulps may include mechanical, chemical, or semi-chemical pulps. Enzymes typically include hydrolases such as cellulases, hemicellulases, pectinases, amylases, and lipases such as esterases, lyases such as pectate lyases, and oxidoreductases. Adsorbents include activated bentonite, microparticles, talc, clay and modified silica. Absorbents typically include water soluble polymers, dispersants, coagulatants and agglomerants.

The present invention relates to a passiflora coerulea and honey peach complex juice and a preparation method thereof. The complex juice consists of 20-30% passiflora coerulea juice, 10-15% honey peach juice, 3-5% honey and water and the preparation method of the complex juice is made of the following steps: washing, cleaning and cutting the passiflora coerulea, removing the fruit pulp with seeds to squeeze juice, washing and cleaning honey peaches, peeling honey peaches to squeeze juice, mixing the two juices evenly and heating to a temperature of 20-60 DEG C, adding pectinase and amylase to conduct enzymolysis, filtering the juice and adding water and honey to flavor, removing parts of bacteria with sterilizing filtration membranes; then homogenizing and degassing the sterilized juice, and vacuum packaging and treating the packaged juice with super-high pressure sterilization and enzyme inactivation treatment, thereby obtaining the complex juice. The preparation method of the complex juice can maintain the unique flavor of passiflora coerulea and delicate fragrance of honey peaches, thus the prepared complex juice has authentic taste of the raw juice, natural and lingering flavor, and improved the quality of fruit juice, and meet the pursuit of natural fruit juice of the majority of consumers.

The invention relates to the technical field of biology, and discloses a moringa extract as well as a preparation method of the moringa extract and a moringa feed additive. According to the preparation method, water is added into moringa leaves, the pH value and the temperature are reduced, then, biological enzymes are adopted for enzymolysis, next, heating digestion is adopted to obtain water extraction liquid, ethanol precipitation is carried out after water extraction liquid concentration, obtained precipitates are the moringa extract, the biological enzymes are protease, cellulose and pectinase, and the protease is papain, pepsase, trypsin or serrapeptass. The enzymolysis effects of the specific protease, cellulose and pectinase is utilized for hydrolyzing large blocks of complicated-molecular-structure ingredients of the moringa leaves per se into smaller water-soluble high-bioavailability small molecular active substances, and then, high-yield and high-quality moringa extracts are obtained through a water extraction and ethanol precipitation method.

The invention belongs to the technical field of feed additives, and in particular relates to complex enzyme for a feed. The complex enzyme comprises 7,500 to 15,000U/g of xylanase, 1,000 to 3,000U/g of mannose, 50 to 80U/g of galactosidase, 200 to 700U/g of cellulose, 500 to 1,500U/g of beta-glucanase, 150 to 1,000U/g of alpha-amylase, 2,000 to 10,000U/g of protease and 100 to 200U/g of pectinase. By adding corresponding enzyme preparations, the anti-nutrition function of the complex enzyme is reduced, the feed utilization is improved, and the animal production efficiency is improved, so the problems that the utilization rate of animal feed raw materials is low and the animal production performance is inhibited are effectively solved; meanwhile, the complex enzyme has the advantages of reasonable raw material mixing, good effect, and low overall cost.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention relates to a natural tea essence prepared by using tea and a preparation method thereof. The tea is taken as a raw material; high-purity natural tea essences with different tea aroma characteristics are prepared through compound enzymolysis, releasing, steam distillation under reduced pressure, cold trap recycling, low temperature freezing concentration, centrifugal oil-water separation, and trace moisture adsorption by using anhydrous sodium sulfate; and the compound enzymolysis and the releasing are realized by adding pectinase, cellulase or pectinase and cellulose complex enzyme, and beta-glucosidase. In the preparation method, a pectinase and cellulose complex enzyme method is adopted for enzymolysis to facilitate cracking cell walls of the tea so as to facilitate liberation and separation of aroma components from the tea, and improve the yield of the essence; therefore, the method is a method for industrially producing the natural tea essence which is energy-saving, low in cost, environmentally friendly and high in efficiency.

A bio-enzyme consists of pectinase, protease, lipase, cellulose, catalase and amylase; the application of bio-enzyme catalysis in residual sludge treatment is that: the residual sludge is treated by utilizing the high-efficiency biocatalysis of the bio-enzyme, odor molecules in the sludge are decomposed and deodorized through enzyme technology, pathogenic bacteria, ova and larvae are killed by utilizing an acid environment formed by inorganic acid generated by the sludge and a high temperature environment in the fermentation process, heavy metal ions in the sludge are stabilized by utilizing the chelation of the bio-enzyme, and the sludge is catalyzed by the enzyme, so that the sludge and water are quickly separated, a large amount of supernate is discharged, the sludge volume is reduced, and the sludge in inland river is reduced and is subjected to innocent treatment; meanwhile, through the bio-enzyme catalysis, long-chain molecules in the sludge are converted into short-chain molecules, polysaccharide and cellulose are converted into protein and amino acid, nutritional components of organic fertilizers are remained, the fertilizer efficiency is improved and the fermentation period can be shortened.

The invention discloses a laying hen composite premix, and belongs to the technical field of feed composition. A composite enzyme preparation containing beta-mannase, beta-glucanase, acidic proteinase, phytase, pectinase, and amylase is adopted to eliminate the anti-nutrient factors in soybean meal. Yeast cell wall type mycotoxin absorbent is used to effectively eliminate the hazardous factors that may affect the intestine health and are caused by using a large amount of corns. A stomach fortifying acid is used to effectively adjust the pH value of intestines. The used wood chips in the premix are soft and high in fiber content, do not hurt the intestines, do not pollute the environment, can effectively strengthen the digesting and absorbing abilities of laying hens, and have a stronger bearing and water-absorbing performance. The materials mentioned above are systematically and scientifically used to guarantee that the intestines of the laying hens be in the best state. The premix can improve the immunity and disease resistant performance of laying hens, and makes the laying hens exert the maximal laying ability. Moreover, the water content of manure is reduced, and the economic profit of farms that use the premix is prominently improved.

The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention discloses a nutritional compound enzyme used for crops, and the application thereof. The nutritional compound enzyme comprises proteases, phytase, amylases, cellulase enzymes and pectinase, and the like, is a compound formula of a plurality of enzyme preparations, can replace a plant growth promoter and improve soil texture, can provide nutritional substances, such as nitrogen sources and phosphorus and the like, for the counterbalanced growth of crops, lead the crops to become strong and absorb sufficient nutrient at the later growth stage, can improve the resistance against diseases of the crops, is beneficial to the growth and maturation of the crops and improves the output of the crops; the nutritional compound enzyme can convert cellulose into a part of oligosaccharides for improving the immunity of the crops and increasing the chlorophyll content of functional leaves of the crops, thereby improving the quality and output of the crops. The nutritional compound enzyme brings significant economic benefits for peasants.

The invention relates to a multienzyme microorganism and plant growth promotion additive. An active regulator containing multienzyme microorganism and leavening, amylase, protease, cellulose, pectase and polyase are used as base stocks, are added with chitin, various elements and vitamins in micro quantity, amino acid and are added with a microorganism and leavening inoculum Em bacterial solution, are mixed and are fermented to prepare the multienzyme microorganism and plant growth promotion additive. The additive can effectively improve soil, strength land capacity, reduce plant diseases and insect pests, fully excavate the soil potential, bring organic nutritive compositions for plants, promote the healthy and rapid growth of the plants, remove negative influence of pesticide and fertilizer, protect soil and entironment, is beneficial for society and people, adopt waste utilization, and has low investment, low cost, rapid effect and good effect.

The invention discloses a preparation method of a mushroom ferment beverage with effect of improving immunity, and belongs to the technical field of food beverages. According to the method disclosed by the invention, mushrooms and/or medicinal plants, fruit and ginseng are adopted as raw materials. The method comprises the following steps: slicing, chopping or crushing the raw materials; uniformly mixing the sliced, chopped or crushed raw materials so as to obtain a mixture; after adding sugar in the mixture, fermenting the mixture in ferment microorganisms; adding sugar when the sugar content is lowered; continuing fermentation so as to obtain the mushroom ferment beverage. According to the method disclosed by the invention, ferment bacteria can regulate intestinal flora and generate SOD (superoxide dismutase), so that the raw materials can be successfully fermented; the prepared ferment beverage contains protease, lipase, SOD, pectinase, lucid ganoderma, cauliflower fungus, pleurotus eryngii polysaccharide, rare ginsenoside, Rb2, C-K and the like. According to the method disclosed by the invention, the fermentation liquor and mycelium of the mushrooms can be directly used for fermentation; compared with sporophore, the raw materials are easier to obtain, and the method has the advantages of being short in fermentation cycle, easy in formula, simple in fermentation process, and the like.

The invention relates to the technical field of straw degradation, in particular to a straw degradation actinomycete and application thereof. The straw degradation actinomycete belongs to streptomyces griseorubens (Streptomyces griseorubens), and the preservation serial number is CGMCC N0.5706. Compared with the prior art, the straw degradation actinomycete and the application have the advantagesthat the streptomyces griseorubens has strong capacity for producing cellulose, hemicellulase, pectinase and ligninase, is free of negative effects on carbon cycling of the nature, and is free of damage to the environment; the streptomyces griseorubens has strong straw degradation capability, enables straw weightlessness rate to achieve 76% after staying in a straw degradation culture medium for 30 days, and is low in culture cost, convenient to operate and easy to industrially apply; and the streptomyces griseorubens has certain antagonism on plant disease microorganism in the soil.

The invention mainly discloses a cotton type woven fabric continuous biochemical pretreatment technology, relating to the technical field of composite preparation of a composite biological enzyme preparation and a composite pretreatment preparation and the application of the composite preparation in the cotton type woven fabric pretreatment technology. The technology comprises the steps of: usingthe composite biological enzyme preparation (the preparation comprises amylase, pectinase, neutral cellulose enzyme, penetrating agent JFC and stabilizing agent) to pretreat the woven fabric, therebypartially or wholly removing starch size, pectin and the other natural or artificial impurities on the woven fabric, and improving the wettability of the woven fabric; and water washing and padding the composite pretreatment liquid (the liquid comprises composite pretreatment agent, hydrogen peroxide and refined penetrating agent); and water washing with steam, wherein the whiteness, the capillary effect, the size removing rate and the strength of a product are better than the normal technology. The technology also solves the problems of high sewage alkalinity, large energy consumption and bad product quality, and the like, in a normal pretreatment technology which takes caustic soda as a main raw material.

The invention discloses a method for brewing dendrobium nobile wine. Dendrobium nobile is used as one of raw materials, corns and potatoes are used as matrixes, and the dendrobium nobile wine is prepared by the procedures of pretreating, mixing, medicine feeding, primary fermenting, secondary fermenting, squeezing, clarifying, wine decocting, inspecting, bottling and storing. During production, the dendrobium nobile serving as the raw material is subjected to combined hydrolysis by using cellulose and pectinase, so that nutrients beneficial to a human body, contained in the Dendrobium nobile,can be separated out fully; and meanwhile, the corns and the potatoes are used as the matrixes, so that the production cost is reduced and simultaneously the prepared dendrobium nobile wine is red brown, has a unique scorch flavor, is mellow, palatable and slightly bitter and has a long-lasting aftertaste. A product of the dendrobium nobile wine has multiple obvious health-care functions of removing lead, enhancing memory ability and immunity, deferring senility, increasing anoxia resistance, preventing osteoporosis and the like. The method is easy to master and can be applied to scale production.

The invention discloses a method for producing table vinegar by fermenting yellow serofluid and bean dregs, which comprises the following steps: dehydrating bean dreg byproducts from bean product processing, and performing puffing pretreatment; sterilizing or boiling yellow serofluid at a high temperature, cooling, adding the treated bean dregs and a cellulase/pectinase composite enzyme preparation, and performing enzymolysis for 24 hours; and adding bean milk and 95% edible alcohol into the enzymolysis solution, inoculating with a Saccharomyces cerevisiae seed solution and an acetic acid bacterium Huniang A.S1.01 seed solution, performing aerobic fermentation at 20-30 DEG C for 5-10 days, centrifuging the matured fermentation solution, filtering, and sterilizing to obtain the finished table vinegar product. The method is low in cost, high in return, short in production cycle, high in equipment utilization rate and huge in market potential, avoids using grain materials, can relieve argument and scruple of all levels in the society about safety of table vinegar (industrial glacial acetic acid blending phenomenon), and saves grains.

The invention discloses an alkaline pectinase production method and application in papermaking pulping. The alkaline pectinase production method comprises the steps of: selective breeding of a bacterial strain, culturing of the bacterial strain, fermentation through shaking a flask, and preparation of an enzyme liquid. The method is characterized in that the bacterial strain subjected to the selective breeding is achieved through separated screening of a naturally acquired bacterial sample and mutagenesis of the bacterial strain at cellular level, i.e. heat-resistant Bacillussubtillis with a laboratory number of MAPLE61 and preservation number of CCTCC NO:M2010004; and when the heat-resistant Bacillussubtillis is used for papermaking pulping, raw materials are soaked with an alkaline pectinase solution after the pickling treatment. When the invention is used for papermaking pulping, the prepared chemical pulp has favorable strength property and bleaching property; and after simple oxidation chlorine bleaching, the whiteness of the pump can reach 80-90 percent, breaking length can reach 6000-7000m, and meanwhile, alkali consumption is reduced about 20-40 percent and the production cost is reduced about 20 percent.

The invention discloses a preparation method of yak bone collagen peptide. The method is characterized by comprising the following technological processes: pretreatment of raw materials, gelatin extraction, separation of a collagen extracting solution, composite enzymatic hydrolysis, enzyme deactivation and sterilization, separation of a peptide solution, decoloring and deodorizing, concentrating and drying. The compound enzyme for composite enzymatic hydrolysis is prepared from protease, amylase, pectinase, cellulose and xylanase. The method has the advantages that the gelatin extraction process is simple, oil and gelatin are completely separated by using flow of a mixed solution, the enzymatic hydrolysis temperature is 48-52 DEG C, the time is 4-6 hours, the temperature is reduced in comparison with common enzymatic hydrolysis temperature, the preparation time of the product is shortened, and the method comprises enzyme deactivation and sterilization procedures, and decoloring and deodorizing procedures, so that the product is good in taste, and applicable to preparation of the yak bone collagen peptide.