1215 results about "Pectinase" patented technology

The invention relates to oral liquid for tonifying kidney and bone and a preparation method thereof, and belongs to the technical field of medicine, the oral liquid contains rhizoma drynariae, polygonum multiflorum, poria cocos, teasel root, radix paeoniae alba, angelica sinensis, codonopsis pilosula, prepared rehmannia root, polygonatum kingianum, fructus lycii, native copper and pericarpium citri reticulatae; the rhizoma drynariae is a product obtained by performing enzymolysis on a whole rhizoma drynariae plant through hemicellulase and pectinase, performing enzymolysis through bromelain and papain and performing enzymolysis through nattokinase; polygonum multiflorum is a product obtained by carrying out enzymolysis on Polygonum multiflorum tuberous roots through hemicellulase and pectinase, then carrying out enzymolysis through bromelain and papain and then carrying out enzymolysis through gelatinase. Polygonatum sibiricum is a product obtained by performing enzymolysis on rhizome of Polygonatum sibiricum through hemicellulase and pectinase, performing enzymolysis through bromelain and papain, and performing fermentation through bacillus licheniformis and saccharomyces boulardii. All the active components are matched for use, kidney function metabolism can be well enhanced, bone mineral density is increased, the treatment course is short, the effect taking speed is high, safety and reliability are achieved, and medication compliance is effectively improved.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention relates to the field of microorganisms, and discloses an Aspergillus oryzae strain and application thereof.The Aspergillus oryzae can produce protease, aminopeptidase, glutaminase, cellulase, amylase and pectinase at high yield, and when the Aspergillus oryzae is applied to soy sauce brewing, the utilization rate of raw materials can be increased, the content of amino acid in soy sauce can be increased, and the flavor of the soy sauce can be increased.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The preparation method comprises the following steps: S1, crushing cordate houttuynia rhizomes, adding the crushed cordate houttuynia rhizomes into a mixed acid aqueous solution, and carrying out immersion treatment to obtain a cordate houttuynia mixed solution; s2, adjusting the pH value of the herba houttuyniae mixed solution to 4-5, adding pectinase, carrying out primary enzymolysis and enzyme deactivation, adjusting the pH value to 6-7, adding compound enzyme, carrying out secondary enzymolysis and enzyme deactivation, so as to obtain an enzymatic hydrolysate; s3, menthol is added into the enzymatic hydrolysate, water extraction is conducted, and water extract and filter residues are obtained; s4, the filter residues are added into ethyl alcohol, then salicylic acid and diacetyl tartaric acid ester of mono (di) glycerides are added, alcohol extraction and filtering are conducted, and an alcohol extraction solution is obtained; s5, mixing the water extract with the alcohol extract, and performing reduced pressure distillation to obtain the herba houttuyniae extract. According to the method, the extraction rate of polysaccharide and flavone in houttuynia cordata can be effectively increased, and meanwhile, the prepared houttuynia cordata extract has better oxidation resistance and has a good application prospect.

The invention relates to the technical field of fruit wine, in particular to herbal fresh fruit wine and a brewing process thereof. The preparation method comprises the following steps: juicing juicy peaches, apples and raspberries, and adding water for blending; the preparation method comprises the following steps of: adding cellulase and pectinase for primary enzymolysis, adding papain and bromelain for secondary enzymolysis, performing enzyme deactivation and sterilization, inoculating bacillus licheniformis CI CC 24553, lactobacillus plantarum CI CC 21794 and saccharomyces cerevisiae CI CC 1406 for fermentation, and performing post-treatment to prepare the herbal fresh fruit wine. The herbal fresh fruit wine prepared by the invention has the effects of excellent oxidation resistance, high total sugar content and excellent smell and taste, and has better use experience.

The invention provides an ultrasonic-assisted red pomelo polyphenol extraction method and application. The polyphenol component in the red pomelo is extracted through the following steps: firstly, performing activation pretreatment on red pomelo dry powder by adopting a low-temperature plasma device, and destroying a cell wall structure so as to improve the accessibility of combined polyphenol; then cellulase, pectinase, hemicellulase and beta-glucosidase are added into a biodegradable deep eutectic solvent to synergistically hydrolyze the cell wall polysaccharide, and combined polyphenol is released; then applying pulse type ultrasonic wave to assist extraction under a low-temperature condition, so that polyphenol is fully dissolved out and enters the eutectic solvent; and finally, carrying out solid-liquid separation to obtain the red pomelo polyphenol extract. Compared with a conventional heat extraction method, the method has the advantages that the extraction rate of the polyphenol of the red pomelo is remarkably increased at a lower temperature, the degradation of the polyphenol is effectively avoided, and the content of the total polyphenol and the antioxidant activity are increased; the method is green and environment-friendly, and can be applied to the health industry as an antioxidant.

The invention relates to the field of microorganisms, and discloses an Aspergillus tubingensis strain and application thereof in preparation of meal seasonings, the Aspergillus tubingensis strain is not subjected to mutagenesis and acclimatization and has the capacity of high yield of glutaminase, feruloyl esterase, glucosidase, pectinase and protease, and when the Aspergillus tubingensis strain is applied to soy sauce brewing, the yield of soy sauce is increased. The high-value utilization of the meal raw materials can be realized, the amino acid content in the soy sauce is increased, and the flavor and nutritional value of the soy sauce are enhanced.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses a method for extracting curcumin from turmeric, which comprises the following steps: adding a deep eutectic solvent solution into turmeric powder, adding pectinase, uniformly mixing, adding terpilenol, performing magnetic stirring extraction and centrifugation, and taking an upper solution, thereby obtaining the curcumin. According to the method, a solvent system combining a deep eutectic solvent (DES), pectinase and terpilenol is adopted, extraction and enrichment of curcumin in turmeric can be achieved through a simple one-step method, the process is greatly simplified, recycling and reutilization of reagents are achieved, and compared with an existing extraction method, the extraction method has the advantages of being easy to operate, green, environmentally friendly, high in yield and the like. The method is short in extraction time, low in production cost and suitable for large-scale industrial production.

The invention belongs to the technical field of biology, and relates to a combined method for improving the taste of cerasus humilis clear juice and increasing the content of available calcium and zinc, which comprises the following operation steps: S1, removing impurities from cerasus humilis fruits, cleaning, denucleating and crushing to obtain cerasus humilis pulp; s2, performing primary enzymolysis on the cerasus humilis fruit pulp to obtain primary enzymolysis cerasus humilis fruit pulp; s3, fermenting the cerasus humilis fruit pulp to obtain fermented cerasus humilis fruit pulp; s4, performing secondary enzymolysis on the fermented cerasus humilis fruit pulp to obtain secondary enzymolysis cerasus humilis fruit pulp; and S5, filtering, sterilizing and filling the secondary enzymolysis cerasus humilis pulp to obtain a cerasus humilis clear juice product. The combined method of high-speed dispersion machine release, pectinase enzymolysis, issatchenkia terricola fermentation and joint enzymolysis of cellulase, beta-glucosidase and alpha-rhamnosidase is utilized, the product taste is effectively improved while the yield of the cerasus humilis clear juice product is increased, and the cerasus humilis clear juice product is palatable in sourness and sweetness, clear, transparent and bright, full in flavor and suitable for large-scale production. And the contents of calcium and zinc are increased by more than 65% and 59%. The product has good calcium and zinc supplementing effects.

The invention discloses a ganoderma lucidum edgeworthia chrysantha promoting agent for promoting efficient edgeworthia chrysantha of aquilaria plants, and belongs to the technical field of edgeworthia chrysantha of aquilaria plants. The component A is prepared from the following components in parts by weight: 20 to 40 parts of aspergillus niger microbial fermentation liquor, 15 to 30 parts of verticillium fulvum microbial fermentation liquor, 10 to 15 parts of fusarium oxysporum microbial fermentation liquor, 5 to 10 parts of ganoderma lucidum extract, 6 to 8 parts of seaweed extract, 2 to 8 parts of malic acid, 3 to 6 parts of fructose, 1 to 3 parts of pectinase and 0.5 to 2 parts of cellulase. Through the synergistic effect of the component A and the component B, the agilawood formation rate and quality of agilawood are effectively improved, the synergistic effect of microorganisms stimulates the agilawood formation potential of plants, accurate regulation and control of the plant hormone xylan emulsion provide conditions for synthesis and accumulation of agilawood substances, and compared with a traditional agilawood formation method, the agilawood formation method has the advantages that the agilawood formation rate is increased, and the agilawood formation quality is improved. According to the method, the agilawood with higher quality can be obtained within shorter time, and the yield is increased.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention belongs to the technical field of biological fermentation and cosmetics, and particularly relates to a cortex magnoliae officinalis fermented product with oil-controlling and dandruff-removing effects as well as preparation and application of the cortex magnoliae officinalis fermented product. The preparation method comprises the following steps: performing ethanol ultrasonic extraction on officinal magnolia bark powder to obtain an officinal magnolia bark extract; adding mangnolia officinalis, honeysuckle, green tea and pericarpium citri reticulatae powder into pure water, performing enzymolysis treatment by adopting cellulase and pectinase, then adding a carbon source, prebiotics and inorganic salt, performing high-temperature sterilization, performing cooling, then adding a mangnolia officinalis extract, and performing uniform mixing so as to obtain a fermentation culture medium; the method comprises the following steps: inoculating an aspergillus seed solution and a saccharomyces cerevisiae seed solution into a fermentation culture medium for first-step fermentation, heating and inactivating, cooling, inoculating fruit wine yeast for second-step fermentation, and sterilizing at high temperature, cooling and filtering after fermentation to obtain filtrate which is the cortex magnoliae officinalis fermented product with the effects of controlling oil and removing dandruff. According to the preparation method, enzymolysis and fermentation technologies are combined, and the honeysuckle, the green tea and the pericarpium citri reticulatae are fermented together, so that the antibacterial, anti-dandruff and oil-control effects of the mangnolia officinalis can be synergistically improved.

The invention relates to the technical field of deep processing of paeonia ostii, in particular to paeonia ostii peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis as well as a preparation method and application of the paeonia ostii peptide. The paeonia ostii flower peptide capable of whitening, moisturizing, tightening and promoting collagen synthesis is prepared through water bath, homogenization, alkali extraction and acid precipitation pretreatment, three times of enzymolysis through compound enzyme of acid protease, cellulase and pectinase and alkaline protease and solid-liquid separation, operation is easy, the requirement for equipment is low, and the paeonia ostii flower peptide is suitable for industrial large-scale production. The prepared paeonia ostii flower peptide has good inhibition capability on tyrosinase, hyaluronidase and elastinase, and also has excellent anti-saccharification and anti-inflammatory effects. Besides, five paeonia ostii active peptide fragments are screened out, are high in activity and non-toxic, can be combined with tyrosinase, hyaluronidase, MMP-1, MMP-3 and COX-2 receptor protein, and have the effects of whitening, moisturizing, tightening and promoting collagen synthesis.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention relates to the technical field of breeding feed, and discloses an efficient feed additive based on emblic leafflower residue component optimization and a preparation method thereof. Comprising the following components in parts by weight: 30-60 parts of emblic leafflower residues, 10-20 parts of amino acids, 5-15 parts of a vitamin premix, 3-8 parts of a mineral premix, 5-20 parts of probiotics, 0.6-4 parts of cellulase, 0.45-3 parts of pectinase and 0.75-4 parts of amylase. By adding the emblic leafflower residues, the nutrition of the feed additive is enriched, the emblic leafflower residues are rich in cellulose, polyphenol, flavone and other components, have an antioxidant effect, can help animals to remove free radicals in bodies, enhance the antioxidant capacity of the bodies and improve the immunity, meanwhile, the emblic leafflower residues are wide in source and relatively low in cost, and as a feed additive component, the feed cost is reduced; the resource utilization rate and culture benefits are improved.

The invention relates to the technical field of illicium verum wine production, in particular to fermented illicium verum wine and a preparation process thereof. Comprising the following components in parts by weight: 60-80 parts of water, 5-10 parts of acidified star anise, 0.5-2 parts of a balm extract, 0.1-0.5 part of sulfurous acid, 0.1-0.5 part of activated pectinase, 0.5-2 parts of active dry yeast, 1-3 parts of a thiamine nutritional agent, 10-20 parts of white granulated sugar, 0.5-2 parts of bentonite GB and 0.1-0.5 part of casein. In the design, after the star anise is acidified, the taste of the wine can be improved, the wine is mellower and softer, and the complexity and layering sense of aroma can be increased; carbonic anhydrase mainly acts on generation and decomposition of carbonate ions, so that the risk of acid residue can be reduced, and other beneficial components or flavor substances in illicium verum are not interfered; the melissa officinalis extract is rich in various volatile aromatic compounds, so that the wine body can be endowed with unique elegant fragrance and complex layering sense, and the overall flavor experience is enhanced.

The invention provides an enzymolysis method for efficiently preparing seaweed oligosaccharide from sargassum, and belongs to the technical field of high-value utilization of seaweed. According to the enzymolysis method provided by the invention, an enzyme composition consisting of algin degrading enzyme, pectinase and cellulase is used for carrying out enzymolysis reaction on the sargassum powder, the enzymolysis rate of the obtained sargassum solid matter reaches 70% or above, and the proportion of high-activity oligomeric alginate oligosaccharide in the enzymatic hydrolysate reaches 80%. According to the method, the synergistic effect of three seaweed degrading enzymes in the same reaction system is realized, the enzyme dosage and cost are reduced, the technical process of sargassum enzymatic degradation is greatly simplified, and the yield of oligomeric alginate oligosaccharide is also effectively improved while the enzymolysis rate of sargassum solids is effectively improved.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The invention provides a cottonrose hibiscus extract as well as an extraction method and application thereof. The extraction method comprises the following steps: S1, performing crushing treatment on hibiscus mutabilis calyx, and then performing extraction treatment to obtain hibiscus mutabilis flower residues; s2, sequentially performing fermentation treatment, enzymolysis treatment and filtration treatment on the hibiscus mutabilis flower residues to obtain the hibiscus mutabilis extract, a strain used in the fermentation treatment is selected from at least one of saccharomyces cerevisiae, bacillus subtilis and lipomyces starkeyi; wherein an enzyme used in the enzymolysis treatment is selected from one of alkaline protease, cellulase and pectinase. According to the extraction method disclosed by the invention, active ingredients are extracted from the hibiscus mutabilis flower residues, and the obtained hibiscus mutabilis extract has relatively high yield and relatively high polypeptide content.

The invention relates to the technical field of pollen polysaccharides, and discloses a preparation method and application of rape pollen polysaccharides, and the preparation method comprises the following steps: treating degreased rape pollen with a mixed enzyme solution of cellulase and pectinase, and centrifuging to obtain a polysaccharide-containing supernatant; mixing the supernate with absolute ethyl alcohol, centrifuging, and dissolving the precipitate with distilled water to obtain a polysaccharide solution; the polysaccharide solution is sulfonated and modified by a chlorosulfonic acid and pyridine mixed solution, and then carboxymethylated with a monochloroacetic acid and NaOH mixed solution to obtain a modified polysaccharide solution. And finally, dialyzing to remove impurities, and freeze-drying to obtain the rape pollen polysaccharide. Through the extraction and modification technology, the application range of polysaccharide products in agriculture is expanded, crop growth can be more effectively promoted, the yield and quality are improved, and meanwhile negative influences on the environment are reduced.

The invention provides a modification method of a ramie fabric, which comprises the following steps: sequentially treating and modifying the ramie fabric by three stages of pectinase, complex enzyme comprising xylanase, hemicellulase, cellulase and laccase, and alkaline protease, so that hairiness on the surface of the ramie fabric is greatly reduced, and the scratchiness is greatly reduced. The wearing experience feeling of the textile taking the ramie fabric as the raw material is greatly improved.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention provides a preparation method of dendrobium leaf-Kangpu tea, which comprises the following steps: crushing dendrobium leaves, adding cellulase and pectinase for enzymolysis treatment, mixing with tea leaves and purified water, heating and boiling, cooling, filtering to remove residues to obtain a leaching solution, and fermenting with a kombucha solution to obtain the Kangpu tea. The obtained Kangpu tea is simple and efficient in processing and making method, unique in flavor, rich in bubbles and rich in nutrition, and can be put into industrial production.

The invention belongs to the technical field of feed additives, and particularly provides a compound preparation for improving the digestibility of dry coconut shreds, which comprises a compound enzyme preparation, the compound enzyme preparation comprises xylanase with the enzyme activity of 0.5 million U / g to 25 million U / g, glucanase with the enzyme activity of 0.8 million U / g to 200 million U / g, mannase with the enzyme activity of 10,000 U / g to 200 million U / g, cellulase with the enzyme activity of 1.2 million U / g to 25 million U / g, pectinase with the enzyme activity of 10,000 U / g to 25 million U / g, galactosidase with the enzyme activity of 10,000 U / g to 300 million U / g and phytase with the enzyme activity of 0.8 million U / g to 200 million U / g. According to the compound preparation provided by the invention, macromolecular substances such as starch in the dry coconut shreds can be degraded into micromolecular substances which are easy to digest, so that the digestion utilization rate is greatly increased; fat is degraded, fatty acid components such as palmitic acid, oleic acid and lauric acid in the coconut shreds are released, and the effects of reducing blood pressure, promoting digestion and the like are achieved; meanwhile, the generated active substances can improve the disease resistance of animals, improve the health state of organisms, improve the nutritional value of the dried shredded coconut and increase the variety of unconventional feed raw materials.

The invention discloses a preparation method of a whole-bean rumex hanus nutrient plant enzyme, which comprises the following steps: S1, taking soybeans, and preparing whole-bean minced soybean; taking rumex hanus to prepare rumex hanus whole pulp; taking grains, and preparing whole grain pulp paste; taking fruits and vegetables, and preparing fruit and vegetable puree; s2, mixing the whole-bean minced soybean, the rumex hanus whole pulp, the whole-grain minced pulp and the fruit and vegetable primary pulp obtained in the step S1 to obtain whole-bean rumex hanus nutrient plant enzyme primary pulp, and then sequentially performing enzymolysis by combining cellulase with pectinase, amylase, saccharifying enzyme and protease; and S3, taking the whole-bean rumex hanus nutrient plant enzyme primary pulp subjected to enzymolysis in the step S2, performing anaerobic fermentation by using saccharomycetes and / or distiller's yeast under the negative pressure condition of-0.1 to-0.06 MPa, and filtering to obtain the whole-bean rumex hanus nutrient plant enzyme. The invention further provides the whole-bean rumex hanus nutrient plant enzyme. According to the technical scheme, the defects of traditional bean products, rumex hanus food and edible plant enzymes can be overcome.