456 results about "Pectinase" patented technology

The present application belongs to the technical field of biological fermentation, and discloses a natural plant fermentation liquor, which is prepared from acanthopanax, angelica sinensis, rosa davurica, phellinus baumii, tea leaves, water, mixed enzymes and mixed bacterial agents; wherein the mixed enzymes include cellulase, pectinase and tannase, and the mixed bacterial agents include lactobacillus plantarum, schizosaccharomyces and lactobacillus acidophilus.The natural plant fermentation liquor has high safety, good clarity after adjusting the concentration of crude drugs, and can be added into a cosmetic matrix.The preparation method is simple, and the effective active ingredients of the natural plants and tea leaves are extracted by the synergistic effect of bacteria and enzymes to the maximum extent.In addition, the present application also discloses the application of the natural plant fermentation liquor in preparing cosmetics for treating oily sensitive skin.

The invention belongs to the technical field of food packaging and fresh-keeping materials, and discloses an ethylene adsorption and pH / pectinase double-response intelligent antibacterial composite film as well as a preparation method and application thereof. The composite film comprises a base film and a functional carrier distributed in the base film, the functional carrier is a material ZIF-8-cinnamyl aldehyde-pectin capable of adsorbing ethylene and responding to pH and pectin, and the base film comprises a film-forming polymer and a natural antibacterial agent. According to the composite film, efficient adsorption of ethylene released in the storage period of fruits and vegetables is achieved through a porous structure of ZIF-8, meanwhile, a synergistic effect is formed by an acid environment naturally generated in the fruit and vegetable ripening and rotting process and pectinase, and intelligent slow release of cinnamyl aldehyde loaded on ZIF-8 is accurately triggered; cinnamyl aldehyde can form a double-channel intelligent antibacterial system with the natural antibacterial property of chitosan in a film base material and the metal ion antibacterial property of ZIF-8, maturation and aging of typical respiratory climacteric fruits and vegetables can be remarkably delayed, and the antibacterial film has extremely high industrial application potential.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention relates to the technical field of traditional Chinese medicine effective component extraction, and provides an efficient extraction and purification process and application of icariin in plateau herba epimedii, the efficient extraction and purification process of icariin in plateau herba epimedii comprises the following steps: S1, epimedium leaves are crushed and sieved to obtain epimedium powder; s2, adding an ethanol solution into the herba epimedii powder, then adding a compound enzyme, performing ultrasonic treatment, inactivating the enzyme, performing suction filtration, and collecting filtrate to obtain an icariin crude extract; s3, diluting the crude icariin extract, adjusting the pH value to 5-6, carrying out chromatographic purification and elution, and collecting the eluate; s4, performing vacuum concentration on the eluent, and performing spray drying to obtain a pure icariin product; the compound enzyme comprises cellulase, pectinase and alpha-mannosidase in a mass ratio of 3: 1: (0.2-1.0). By means of the technical scheme, the problem that in the prior art, the extraction rate of icariin in plateau herba epimedii is low is solved.

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The invention relates to a multi-component ginseng extract containing ginseng polypeptide and a preparation method of the multi-component ginseng extract. The preparation method comprises the following steps: S1, preparing ginseng powder from a ginseng raw material; mixing the ginseng powder with an acidic buffer solution, and carrying out presoaking treatment and homogenate dissolution promoting treatment to obtain ginseng primary pulp; s2, mixing the wall-breaking enzyme system with the ginseng primary pulp, and performing acid wall-breaking treatment to obtain wall-broken primary pulp; mixing an invertase system with the wall-broken primary pulp, and carrying out enzymolysis synchronous glucoside conversion treatment to obtain a crude extract; s3, filtering and separating the crude extracting solution to obtain an extract containing the ginseng polypeptide; the pH values of the ginseng primary pulp, the wall-broken primary pulp and the crude extract are in a range of 4.0-6.0; the wall breaking enzyme system comprises pectinase and cellulase; the invertase system comprises beta-glucosidase and acid protease. The technical problems that in the prior art, a ginseng extract is complex in extraction process, multiple active ingredients are difficult to efficiently extract at the same time, the product quality is low, and the biological activity is poor are solved.

The invention belongs to the technical field of natural product extraction and enzyme engineering, and particularly relates to a method for extracting red pomelo polyphenol based on an enzyme method. The method aims at solving the problems of high cost and the like caused by easy inactivation and difficult recovery of enzymes in most red pomelo extraction processes. Waste such as pericarp and pomelo seeds generated by red pomelo processing are used as carbon sources, a biochar material with a hierarchical porous structure is prepared through programmed heating carbonization activation, pectinase, cellulase and naringinase are immobilized by adopting a spatial zoning sequential immobilization strategy and then react with the red pomelo, and the biochar material with the hierarchical porous structure is prepared. The immobilized enzyme is rapidly recovered through magnetic separation to realize repeated utilization of the enzyme, and finally red pomelo polyphenol powder is obtained through multi-step treatment, so that recycling is realized, and the yield of polyphenol extracted by an enzyme method is improved.

The invention discloses a leontopodium alpinum callus extract as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. The leontopodium alpinum callus extract is prepared by the following steps: mixing leontopodium alpinum callus freeze-dried powder with a buffer solution, adding cellulase, pectinase and beta-glucanase, uniformly mixing, reacting to obtain primary enzymolysis slurry, adjusting the primary enzymolysis slurry to be neutral, adding phospholipase A2, stirring, reacting to obtain deep enzymatic hydrolysate, filtering, washing, and drying to obtain the leontopodium alpinum callus extract. Transferring into an extraction kettle, adding an entrainer, and extracting to obtain a leontopodium alpinum extract; dissolving soybean phospholipid and cholesterol in ethanol to obtain an organic phase; dispersing the leontopodium alpinum extract in a buffer solution to obtain an active matter water phase; and injecting the organic phase into the active matter water phase, stirring, carrying out reduced pressure distillation, and homogenizing. The preparation method provided by the invention can efficiently and completely obtain the active component, and the nano-liposome formed by phospholipid molecules can effectively improve the stability of the active component.

The invention belongs to the technical field of plant fermented beverages, and particularly relates to a synergistically fermented composite plant beverage and a preparation method thereof. The preparation method comprises the following steps: sieving platycodon grandiflorum and liquorice, and then carrying out ultrasonic pretreatment and cold extraction to obtain a platycodon grandiflorum and liquorice cold extraction solution; adding pectinase and beta-glucosidase into the pear juice, and carrying out enzymolysis composite treatment, so as to obtain pear juice after enzymolysis; mixing the platycodon grandiflorum and liquorice cold extraction liquid and the pear juice subjected to enzymolysis to obtain a cold extraction mixed liquid, and then inoculating a combined strain for fermentation to obtain a fermented mixed liquid; and performing molecular distillation and concentration on the fermentation mixed solution, and then performing high-pressure carbon dioxide sterilization and photodynamic sterilization in sequence to obtain the product. According to the preparation method disclosed by the invention, the preparation time is effectively shortened, the energy consumption is reduced, the preparation efficiency is improved, and the prepared composite plant beverage is high in content of active ingredients and nutrient elements such as GABA, platycodin and the like, long in storage time and high in storage stability.

The invention relates to an extraction process of total flavonoids of gold-edge roses, and aims to solve the technical problems of low extraction rate, easiness in pyrolysis inactivation of components and poor stability in the existing extraction technology. The invention provides an enzymatic wall breaking-in-situ inclusion synergistic extraction scheme, which is technically characterized in that in a pure water system, the directional degradation effect of pectinase on a cell wall pectin matrix is utilized, and in-situ encapsulation protection of beta-cyclodextrin on dissolved active molecules is matched; enzymolysis and clathration processes are synchronously executed under a mild temperature condition, so that instantaneous steady-state locking is carried out on heat-sensitive flavone while physical wall breaking is realized. According to the process, the yield of total flavonoids is remarkably increased, oxidative degradation in the extraction process is effectively inhibited, and the obtained product is good in water solubility and physical and chemical stability. No organic solvent is needed in the whole process, conditions are mild, and a new path is provided for efficient extraction of active ingredients of the gold-edge roses.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The invention relates to the technical field of biology, and discloses high-activity black fungus dry powder as well as a preparation and a preparation method thereof, and the black fungus dry powder is prepared by the following steps: selecting high-quality black fungus in northeast as a raw material, and performing primary washing, fine washing, color protection and draining pretreatment at normal temperature in a dark place; carrying out compound enzymolysis by using cellulase and pectinase under a low-temperature acidic condition; power frequency ultrasonic-assisted extraction is set; drying under a vacuum freezing condition until the moisture is less than or equal to 8%; according to the process disclosed by the invention, the raw materials are selected, and multi-step cooperative treatment of low-temperature enzymolysis wall breaking, ultrasonic enhanced mass transfer, vacuum freeze-drying keep-alive and sterile post-treatment is combined, so that active ingredients of polysaccharide, melanin and triterpenoids are fully reserved, and the process is simple and convenient to operate, low in cost and suitable for large-scale production. Therefore, the extraction efficiency and bioavailability are improved, the hygienic safety and storage stability of a finished product are guaranteed, and a foundation is laid for subsequent development of diversified black fungus preparations.

This invention discloses an integrated micro-aqueous degumming and peeling method for fresh coffee cherries, belonging to the field of fresh coffee cherry processing technology. The method includes: micro-aqueous degumming of fresh cherries using a pretreatment solution containing sodium citrate and sodium chloride; biological degumming using a specialized composite degumming enzyme composed of pectinase, cellulase, and calcium chloride; adding chitosan to the wastewater after rinsing for composite coagulation, followed by solid-liquid separation to obtain recycled water; and finally drying to obtain coffee beans in their shells. This invention utilizes sodium citrate to chelate calcium bridges to degrade the pectin cross-linking structure and sodium chloride to compress the double electric layer and reduce slurry viscosity, achieving efficient mass transfer and rapid degumming under micro-aqueous conditions. Furthermore, it utilizes residual salts to achieve pH anchoring and ionic strength regulation, promoting the formation of a dense aggregated phase between pectin degradation products and chitosan, solving the industry problems of difficult micro-aqueous system treatment and unstable recycled water reuse. This invention reduces water consumption by more than 90% compared to traditional washing methods, achieves thorough degumming, preserves the complete flavor of coffee beans, has low equipment costs, and enables sustainable closed-loop production, making it suitable for industrial application in small-scale coffee processing plants.

The invention belongs to the field of food engineering and traditional Chinese medicine functional foods, and discloses a preparation method of a tremella and ginseng composite freeze-dried product, which comprises the following steps: S1, preparing a decoction, weighing poria cocos, Chinese yam and other medicinal materials and ginseng slices according to a ratio, soaking, taking out the ginseng slices for later use, decocting the remaining medicinal materials, and filtering to obtain the decoction; s2, preparing a radix ophiopogonis zymolyte, namely crushing radix ophiopogonis, dissolving, adding a compound enzyme (cellulase: pectinase = 2: 1) for enzymolysis, and performing enzyme deactivation and concentration to obtain the zymolyte; s3, the tremella is boiled to produce glue, the soaked tremella and the medicine soup are mixed and boiled, and the radix ophiopogonis zymolyte, the ginseng powder and the sweetening agent are added; s4, molding and filling into a mold: filling the white fungus thick soup into the mold, and embedding the soaked ginseng slices, Chinese wolfberry fruits and red date slices; and S5, freeze-drying and packaging, namely sealing after pre-freezing and sublimation drying. According to the preparation method, the rehydration time of the product is less than or equal to 67 seconds, the retention rate of ginsenoside is greater than or equal to 90% and the distribution of ingredients is uniform and visual through segmented ginseng treatment (slice embedding and powder supplementing), radix ophiopogonis enzymolysis and process parameter standardization, so that the preparation method is suitable for industrial production of health-care foods and ready-to-eat foods.

This invention discloses a low-temperature enzymatic hydrolysis compound beverage made from edible and medicinal plants, and its preparation method. Using jujube powder as a base, at least two kinds of dried powders from medicinal and edible plants are compounded. Enzymatic hydrolysis is performed using a compound enzyme containing cellulase, pectinase, and acidic protease at 45-50℃ and pH 4.0-5.0. The product is then subjected to enzyme inactivation and solid-liquid separation (filtration / centrifugation or a combination thereof). Amylase can be added for high-starch / polysaccharide raw materials. The product contains no added flavorings, colorings, sweeteners, or preservatives. After 12 months of storage at room temperature, the sedimentation rate is ≤0.5%, and the retention rate of active ingredients is 26%-31% higher than that of traditional high-temperature processes. The process is highly versatile and can be adapted to different separation methods, enabling the industrial production of clean-label, highly stable plant-based beverages.

The invention discloses a preparation method of a breathable antibacterial composite fabric, and particularly relates to the field of layered fabrics. The method comprises the following steps: by taking poplar fallen leaves as a raw material, crushing, immobilizing by glutaraldehyde, and sequentially treating by pectinase, cellulase and alkaline hydrogen peroxide to form an immobilized cell template with a cell wall microstructure; then, a mixed solution of chitosan, an antibacterial agent and a cross-linking agent permeates into gaps of the template, the antibacterial agent is covalently grafted to the chitosan through a Michael addition reaction, the cell template is removed through EDTA dissolution, and a porous antibacterial layer is reserved; and finally, compounding the keratin microspheres and the silk fibroin-hydroxyethyl methylacrylate copolymer, coating two sides of the antibacterial layer with the compounded keratin microspheres and silk fibroin-hydroxyethyl methylacrylate copolymer, and initiating crosslinking by ultraviolet light to form a compact and breathable microporous coating layer. By means of bionic structure design and a covalent grafting technology, efficient unification of the antibacterial function and the air permeability is achieved.

The present application belongs to the technical field of pulping and papermaking, and provides a method for preparing a cotton-stalk biochemomechanical pulp. The present application provides a method for preparing a cotton-stalk biochemomechanical pulp, the method comprising the following steps: cutting cleaned and dried cotton stalks, and then sequentially performing pre-steaming and a screw extrusion fiberization treatment; immersing the defibrated cotton stalks into a buffer solution of cellulase to perform an enzymatic treatment for a set period of time, and then immersing same into a hot alkali solution; sequentially subjecting the cotton stalks, which have been subjected to a hot alkali impregnation treatment, to high-consistency pulp refining and a latency removal treatment, and then subjecting the resulting coarse pulp to a second-stage enzymatic treatment by using a compound enzyme of xylanase and pectinase; and subjecting the pulp, which has been subjected to the second-stage enzymatic treatment, to pulp screening and PFI beating, so as to obtain a cotton-stalk biochemomechanical pulp.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

This disclosure provides a method for extracting bioactive substances from microalgae in high-salt-alkali environments, specifically including low-temperature plasma pretreatment; mixed enzymatic hydrolysis to break down cell walls, the mixed enzymes including cellulase and pectinase; ultrasonic extraction with a salt-tolerant extract; and purification by aqueous two-phase extraction followed by hydrophobic chromatography. The technical solution disclosed in this invention solves the problems of low extraction rate, high cost, and salt-alkali interference in the existing extraction of bioactive substances from high-salt-alkali microalgae, achieving (1) increasing the extraction rate of bioactive substances from high-salt-alkali microalgae to ≥90%; (2) shortening the purification process to ≤24 hours, with a target product purity of ≥95%; and (3) reducing the interference of the salt-alkali environment on the extraction process and reducing the amount of organic solvent used by more than 30%.

The invention provides a quinoa flavone extracting solution as well as a preparation method and application thereof, and belongs to the technical field of natural plant extraction. The preparation method of the quinoa flavone extracting solution provided by the invention comprises the following steps: mixing quinoa, compound enzyme and water to obtain a mixed solution; performing ultrasonic treatment on the mixed solution to obtain a quinoa flavone extracting solution; the compound enzyme comprises cellulase and pectinase. According to the method, the flavone in the chenopodium quinoa willd is extracted by adopting ultrasonic-compound enzyme synergistically, so that the method has the characteristics of high yield, short extraction time, low energy consumption, large raw material treatment capacity, low possibility of damaging active substances, environmental friendliness, reduction of environmental pollution and the like, the comprehensive utilization of the chenopodium quinoa willd can be enhanced, and the additional value of the chenopodium quinoa willd can be improved.

The invention relates to the technical field of biological waste treatment, in particular to a preparation method and application of a silkworm sand feed additive. The invention provides a technical scheme for preparing a silkworm and mulberry sand feed additive rich in acid protease, pectinase and cellulase by taking silkworm sand as a core matrix and performing directional pretreatment, adaptive strain fermentation and standardized post-treatment. According to the invention, silkworm sand-corncob powder is used as a composite substrate, and the silkworm sand and the corncob powder complement each other in function, so that the problems of unbalanced nutrition and poor air permeability of a single substrate are solved. Specific aspergillus niger is used for segmented solid-state fermentation, parameters are set in a differentiated mode, and full-amount utilization of the silkworm sand is achieved; the product quality and enzyme activity are improved by optimizing the process, and the resource value is improved from the low-added-value field to the high-added-value enzyme preparation industry.

The invention discloses a walnut kernel skin active component enrichment method based on differential pressure blasting puffing and gradient biotransformation, and relates to the technical field of agricultural product deep processing and plant active component extraction, and the method comprises the following steps: S1, carrying out differential pressure blasting puffing treatment on dried walnut kernel skin, maintaining the pressure of the material at 0.8-1.5 MPa for 10-60 seconds, and then instantaneously releasing the pressure to normal pressure within 0.05-0.2 seconds, the porosity is increased, cell walls are broken, and the specific surface area is increased; s2, mixing the puffed walnut kernel skin and water according to a solid-liquid mass ratio of 1: 10-1: 30 to form slurry, adding a compound enzyme system containing cellulase, hemicellulase, pectinase, tannase and / or protease, and performing enzymolysis for 1-4 hours under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-6.0, so that cell wall components are partially hydrolyzed and bound phenols are released; and S3, sequentially carrying out first-stage fermentation and second-stage fermentation on the materials subjected to enzymolysis.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.

The invention relates to the technical field of hippophae rhamnoides fruit craft beer, and discloses a hippophae rhamnoides fruit craft beer production process which comprises five steps of fresh hippophae rhamnoides fruit pretreatment, sterilization and corrosion prevention, sugar degree adjustment, fermentation treatment and post-treatment. In the pretreatment stage, clear fruit juice is obtained through centrifugal impurity removal, chemical and biological step-by-step deacidification, compound pectinase enzymolysis and light-shielding clarification; medium and low temperature pasteurization is adopted for sterilization, and a food-grade preservative reagent is matched to guarantee safety; the sugar degree is accurately regulated and controlled in batches by using compound saccharides to meet the fermentation requirements; the fermentation adopts a low-temperature mode and yeast-lactic acid bacteria combined biological deacidification, and beta-glucosidase and a nano-scale SiO composite reagent are added to optimize the flavor; the finished product is obtained through purification, dynamic clarification, low-temperature freezing, filtering and filling after post-treatment, and VC and EDTA-2Na are compounded in the whole process to prevent browning. The process overcomes the pain points of high acid, low sugar, non-enzymatic browning and the like of the sea-buckthorn, realizes synergy of fruit fragrance and beer flavor, remarkably improves the fermentation efficiency and the yield of finished products, is suitable for industrial production, and retains the nutrition and original flavor of the sea-buckthorn.

The invention provides a paper mulberry fruit natural product efficient preparation method based on ultrasonic extraction, which comprises the following steps: S1, raw material pretreatment: crushing dried paper mulberry fruits, and sieving with a 40-60-mesh sieve to obtain paper mulberry fruit powder; s2, composite enzymatic hydrolysis pretreatment: adding deionized water of which the mass is 0.5-1.5 times of that of the fructus broussonetiae powder into the fructus broussonetiae powder in the S1, uniformly stirring, then adding a compound enzyme of which the mass is 0.1-0.5% of that of the powder, and carrying out enzymatic hydrolysis for 30-90 minutes under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-5.5; the compound enzyme is formed by compounding pectinase and cellulase according to the mass ratio of 1: 1-3: 1, the pectinase and the cellulase are subjected to compounding pretreatment, impurity structures are accurately disintegrated, dissolution of pectin and polysaccharide is reduced from the source, a single-frequency mode is abandoned in an ultrasonic link, and flavone is protected through low-frequency strong cavitation wall breaking, high-frequency mild mass transfer and pulse temperature control. The two methods are not simply superposed, enzymolysis is a foundation for ultrasonic directional extraction, ultrasonic amplification of the enzymolysis effect finally solves the traditional problems of many impurities and low efficiency, and a green and efficient flavone extraction scheme is formed.

The invention provides a preparation method and application of high-palatability carrot dietary fibers. The method comprises the following steps: (1) washing and dicing carrots; (2) cellulase and saccharifying enzyme enzymolysis; (3) constructing a two-phase system, adding a compound enzyme and pectinase, and carrying out gradient enzymolysis; (4) carrying out enzyme deactivation and centrifugation to obtain a water phase; (5) performing gradient centrifugation to obtain mixed fibers; (6) precipitating, and collecting precipitated fibers; (7) adding carrot source nano cellulose to obtain a composite fiber with a core-shell structure; and (8) drying, crushing and sieving. According to the preparation method disclosed by the invention, the core problems of rough taste (large particle size), astringent taste (residual acetyl groups and terpene substances), weak function and low purity in the traditional carrot dietary fiber preparation process are solved through a water-alcohol two-phase gradient composite enzymolysis and nano cellulose composite purification process; and finally, the high-palatability carrot dietary fiber which is fine, smooth, free of rough feeling, natural, fresh and sweet, high in purity and strong in function is prepared.

The invention relates to the technical field of enzyme preparation preparation and fragrance-retaining beads, in particular to an enzyme preparation, a preparation method thereof and a deodorizing and odor-removing fragrance-retaining bead. Wherein the enzyme preparation comprises a compound enzyme microcapsule and a protease microcapsule in a mass ratio of (3-6): 1; a core material of the compound enzyme microcapsule is prepared from the following components in parts by mass: 12 to 15 parts of lipase, 2 to 4 parts of amylase, 5 to 8 parts of fig lysozyme, 2 to 4 parts of cellulase, 1 to 3 parts of pectinase and 1 to 3 parts of phosphodiesterase (PDE). The enzyme preparation disclosed by the invention is added into the aroma-retaining beads for use, so that the antibacterial, deodorizing and peculiar smell removing effects of the aroma-retaining beads can be effectively improved.