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358 results about "Medicinal plants" patented technology

Medicinal plants, also called medicinal herbs, have been discovered and used in traditional medicine practices since prehistoric times. Plants synthesise hundreds of chemical compounds for functions including defence against insects, fungi, diseases, and herbivorous mammals. Numerous phytochemicals with potential or established biological activity have been identified. However, since a single plant contains widely diverse phytochemicals, the effects of using a whole plant as medicine are uncertain. Further, the phytochemical content and pharmacological actions, if any, of many plants having medicinal potential remain unassessed by rigorous scientific research to define efficacy and safety.

Application of medicinal plant synergistic repair technology in functional cosmetics

The invention relates to application of a medicinal plant synergistic repair technology in functional cosmetics. The functional cosmetics comprise the following components in percentage by mass: 0.05-3 parts of peony root bark extract; 0.01 to 3 parts of magnolia flower extract; 0.005 to 0.5 part of a radix scutellariae extract; 0.005 to 0.5 part of a centella asiatica extract; the synergistic component is 0.001-2 parts of radix ophiopogonis root extract, and the preferable mass ratio of the peony root bark extract to the magnolia flower extract to the scutellaria baicalensis extract to the centella asiatica extract is (6-10): (2-4): 1: (1-8). All plant components are natural extracts, do not contain harmful chemical components and are suitable for various skin types, especially sensitive skin, and the soothing and repairing effects are greatly improved through the matching design and synergistic effect of the plant extracts. For example, due to the synergistic effect of the peony root bark extract and the radix scutellariae extract in the anti-inflammatory and antibacterial aspects, the relieving effect on external stimulation is enhanced; the permeability of the compound is enhanced by the radix ophiopogonis root extract, so that the effect is more long-acting.
Owner:BEIJING RIMENG BIOTECHNOLOGY CO LTD

Medicinal plant growth cycle evaluation method based on AI image recognition

The invention relates to the field of intelligent evaluation and management of a medicinal plant growth cycle, and provides a medicinal plant growth cycle evaluation method based on AI image recognition, which comprises the following steps: acquiring a multi-dimensional image sequence of a plant growth process through a multispectral imaging device, and synchronously recording environment illumination intensity and temperature and humidity data; performing space-time registration on the image sequence, and eliminating pixel offset caused by plant swing and shooting angle change; de-noising by adopting an adaptive hybrid filtering algorithm, and reserving leaf edge texture details; performing multi-scale contrast enhancement on the de-noised image, and reconstructing a high-frequency feature map; constructing a double-branch deep network, and respectively extracting a leaf morphological dynamic feature set and a physiological parameter change feature set; performing cross-modal fusion on the two feature sets, and calculating a dynamic growth index matrix in combination with environmental parameters; and based on matching degree analysis of the dynamic growth index matrix and a preset growth stage model, outputting a stage identifier and a stage transition probability curve of the current growth cycle.
Owner:DAZHOU VOCATIONAL & TECH COLLEGE

Application of uncaria acidic polysaccharide URP-2 in preparation of anti-esophageal cancer drugs

The invention discloses application of uncaria acidic polysaccharide URP-2 in preparation of an anti-esophageal cancer drug, and belongs to the technical field of antitumor of medicinal plant polysaccharide. According to the method, a method for extracting the uncaria total polysaccharide by boiling water and precipitating the uncaria total polysaccharide by ethanol is adopted, and a DEAE DE-52 cellulose column and sephadex column purification technology is combined, so that the separation of the uncaria acidic polysaccharide component is realized, and the uncaria acidic polysaccharide URP-2 is obtained. The uncaria acidic polysaccharide URP-2 comprises the following components in mole percent: 14.11% of arabinose, 12.64% of rhamnose, 6.48% of galactose, 5.00% of glucose and 61.77% of galacturonic acid. Experiments show that the uncaria acidic polysaccharide URP-2 has remarkable anti-esophageal cancer activity, so that a potential experimental basis and a new strategy are provided for applying the uncaria acidic polysaccharide URP-2 to clinical treatment of esophageal cancer.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE

SmCLE mature peptide and application thereof in plant stress resistance

The invention discloses an SmCLE mature peptide and application thereof in plant stress resistance, and belongs to the technical field of plant biology. According to the technical scheme, the SmCLE mature peptide is provided, and the amino acid sequence of the SmCLE mature peptide is SEQ ID NO: 1. The CLE peptide is applied to the aspect of plant stress resistance, the problem that an existing CLE peptide cannot be applied to stress resistance of plants, especially medicinal plants or non-mode crops is solved, the CLE peptide has high biocompatibility and targeting performance, and the biomass and yield of the crops in a stress environment can be remarkably improved.
Owner:HEBEI UNIV OF ENG

Establishment method of medicinal plant litsea cubeba tissue culture seedling system

The invention discloses a method for establishing a medicinal plant litsea cubeba tissue culture seedling system. Aiming at the problems of difficulty in explant pollution and browning control, low proliferation and rooting efficiency, insufficient transplanting and domesticating survival rate and the like in establishment of a litsea cubeba tissue culture system in the prior art, the invention provides the establishment method of the medicinal plant litsea cubeba tissue culture seedling system, the germination rate of explants is 90% or above, and the transplanting survival rate in 30 days reaches 90.6%.
Owner:GUANGXI ACADEMY OF SPECIALTY CROPS GUANGXI ZHUANG AUTONOMOUS REGION

Application of SmCLE mature peptide in inhibiting plant aging

The invention discloses application of SmCLE mature peptide in inhibiting plant aging, and belongs to the technical field of plant biology. The invention discloses application of a SmCLE mature peptide in inhibiting plant aging. The amino acid sequence of the SmCLE mature peptide is SEQ ID NO: 1. The CLE peptide is applied to the aspect of inhibiting plant aging, solves the problem that the existing CLE peptide cannot be applied to anti-aging of plants, especially medicinal plants or non-mode crops, has the advantages of small molecular weight, easiness in synthesis, low action concentration and good biocompatibility, can realize accurate regulation and control of leaf aging through exogenous application, and has a wide application prospect. And the safety problem of transgenic organisms is avoided.
Owner:HEBEI UNIV OF ENG

Organic fertilizer suitable for increasing yield and killing pests of tea leaves and preparation method of organic fertilizer

The invention discloses an organic fertilizer suitable for tea yield increase and insect killing and a preparation method thereof, and relates to the technical field of organic fertilizer and microbial fertilizer manufacturing. The organic fertilizer is prepared from straw, chicken manure, radix inulae, clinacanthus nutans, affine cudweed, periploca forrestii schltr, ellagic acid-rice protein zymolyte, sodium laurate modified diatomite, sodium humate, urea, compound microorganism bacteria and water. The compound microorganism bacterium is composed of candida utilis saccharomycetes and neurospora intermedia. The compound microorganism bacteria in the organic fertilizer are beneficial to accelerating fermentation decomposition of the straw, the chicken manure, the sodium humate, the urea and the traditional Chinese medicine plants and promoting macromolecular substances to be converted into micromolecular active ingredients, then ellagic acid-rice protein zymolyte and sodium laurate modified diatomite are supplemented, and the yield of tea tree planting is increased through reasonable proportion of nutritional ingredients.
Owner:郴州市农业科学研究所

Application of LmbZIP11 gene in regulating and controlling content of chlorogenic acid and total flavonoids

The invention relates to a method and application of an LmbZIP11 gene and a homologous NbbZIP11 gene of the LmbZIP11 gene in regulating and controlling the content of chlorogenic acid and total flavonoids in plants. The method comprises the following steps: extracting RNA (Ribonucleic Acid) of leaves of lonicera macranthoides, designing a specific primer, cloning to obtain an LmbZIP11 gene, further constructing a recombinant overexpression vector and a VIGS (Vinyl Insulin Growth Sequence) silencing vector of the LmbZIP11 gene, and respectively transforming arabidopsis thaliana and nicotiana benthamiana; experiments show that the content of total flavonoids and chlorogenic acid in arabidopsis thaliana is remarkably increased through overexpression of the LmbZIP11 gene, and the content of metabolites in tobacco is remarkably reduced through silence of the NbbZIP11 gene homologous with the LmbZIP11 in Benen tobacco; the invention provides a new strategy for regulating the synthesis of plant secondary metabolites by using the LmbZIP11 gene, and can be used for improving the quality of medicinal plants.
Owner:HUNAN AGRI UNIV

Nardostachyos root and rhizome endophytic fungus strain and application thereof

The invention provides a rhizoma nardostachyos endophytic fungus strain and application thereof, the rhizoma nardostachyos endophytic fungus strain is derived from an endophytic fungus of a medicinal plant rhizoma nardostachyos, is separated from the medicinal plant rhizoma nardostachyos by adopting an endophytic fungus separation and purification technology, and is named Fusarium sp.GSJ1 through microbial taxonomic identification, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2025, and the preservation number is CGMCC NO.41765. The invention further discloses a preparation method of the strain. The fungus strain can be used for producing the wollaman penicillin through solid-state fermentation of rice, provides a new way for the source of the wollaman penicillin, and has important production practice value.
Owner:TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE

Method for repairing salt stress damage of cutting seedlings of aromatic medicinal plants

The invention relates to the technical field of plant stress regulation and medicinal plant cultivation. A salt stress damage repair method for cutting seedlings of aromatic medicinal plants comprises the following steps: after asexual cutting seedlings of the aromatic medicinal plants suffer from salt stress, spraying a melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in a repair window period of 3-6 days after the salt stress occurs, spraying 1-2 times a day, continuously spraying for 2-3 days, and continuously spraying the melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in the repair window period of 3-6 days after the salt stress occurs. Melatonin is used for repairing salt damage of the asexual cuttage seedlings of the aromatic medicinal plants. The melatonin can be accurately applied before the salt damage critical point to repair the physiological damage of the cutting seedlings of the aromatic medicinal plants, and the salt damage influence of the cutting seedlings of the aromatic medicinal plants is greatly reduced; the problems that cutting seedlings of the aromatic medicinal plants are prone to salt damage under salt stress, the survival rate is low, and the content of main medicinal components is reduced are solved, and the dual effects of seedling physiological restoration and medicinal component content stabilization can be achieved.
Owner:PINGDINGSHAN IND TECHNOLOGY RESEARCH INSTITUTE OF HENAN ACADEMY OF SCIENCES

Pinellia ternata NAC transcription factor PtNAC61 and coding gene and application thereof

The invention discloses a Pinellia ternata NAC transcription factor PtNAC61 as well as a coding gene and application thereof, belongs to the technical field of genetic engineering, and provides the Pinellia ternata NAC transcription factor PtNAC61 which is any one of the following proteins: (1) a protein consisting of an amino acid residue sequence in SEQ ID NO.1; (2) a protein which is obtained by substituting and / or deleting and / or adding one to ten amino acid residues on the amino acid residue sequence in the SEQ ID NO.1, has a pinellia ternata NAC transcription factor function and is derived from (1); the invention also provides a PtNAC61 gene for coding the NAC transcription factor Pt NAC61 of the pinellia ternate. The PtNAC61 gene is separated and cloned from pinellia ternate, the PtNAC61 gene is introduced into the pinellia ternate through an agrobacterium tumefaciens-mediated transformation method, a transgenic plant is obtained, it is verified that the drought stress tolerance of the transgenic pinellia ternate is improved, the anti-aging capacity of leaves is improved, an important gene resource is provided for plant stress-resistant gene engineering, and the PtNAC61 gene has a wide application prospect. The important significance is realized on improving the yield of medicinal plants.
Owner:HUAIBEI NORMAL UNIVERSITY

Method for cloning medicinal plant stem cells and obtaining high-yield cell line

The invention relates to the technical field of biology, in particular to a method for cloning medicinal plant stem cells and obtaining a high-yield cell line, and the cell line with high yield of specific active substances is obtained through establishment of a suspension cell line, obtaining of single cells, cell cloning culture and screening of cloned cells.
Owner:ZHEJIANG FINDYOU BIOTECHNOLOGY CO LTD

Nondestructive detection method and system for pesticide residues of field drinking chrysanthemum

The invention discloses a nondestructive testing method and system for pesticide residues of field drinking chrysanthemum, and the method comprises the steps: obtaining hyperspectral reflectivity data of leaves of the drinking chrysanthemum in a key growth period after pesticide drip irrigation, and carrying out data preprocessing; a quantitative estimation model of the leaf hyperspectral reflectivity and the leaf clothianidin actually measured content is constructed through statistical modeling; determining a regression model equation of the clothianidin content in the leaves and inflorescence by using actually measured data, and calculating the inflorescence residue level according to a leaf detection result; a drinking chrysanthemum clothianidin residue early warning threshold value is set, the hyperspectral reflectivity of drinking chrysanthemum leaves is collected through hyperspectral equipment and input into the model, and drinking chrysanthemum clothianidin residue early warning is achieved; according to the method, early warning of pesticide residues is achieved before chrysanthemum harvesting, especially field rapid screening and quantitative analysis of novel pesticides such as clothianidin are achieved, the technical blank of an existing hyperspectral technology in pesticide residue detection and application of medicinal plants is filled up, and the chrysanthemum quality safety guarantee level is improved.
Owner:NANJING AGRICULTURAL UNIVERSITY

Extraction method of genome DNA of plant rich in secondary metabolites and buffer solution

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.
Owner:广西农业职业技术大学

Tripterygium wilfordii exosome, preparation method and application of tripterygium wilfordii exosome in preparation of medicine for treating cervical tumor

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.
Owner:QUFU NORMAL UNIV

Culture medium for inhibiting browning of fructus amomi explants

The invention belongs to the technical field of medicinal plant tissue culture, and particularly discloses a culture medium for inhibiting browning of fructus amomi explants, the pH value of the culture medium is 5.0-6.0, the culture medium contains KNO3, NH4NO3, KH2PO4, MgSO4. 7H2O, CaCl2. 2H2O, MnSO4. 4H2O, ZnSO4. 7H2O, H3BO3, VB1, VB6, VB5, soybean mash filtrate, salicylic acid and the like, the formula of the culture medium aims at solving the problem of tissue culture browning of fructus amomi, and extremely low ammonium, organic nitrogen synergism and microelement regulation are adopted. By adopting the culture medium, the browning rate in the villous amomum fruit tissue culture process can be effectively inhibited, the seedling quality and the production efficiency are remarkably improved, the seedling culture period and the seedling culture cost can be greatly reduced in the villous amomum fruit seedling rapid propagation culture process, and a technical guarantee is provided for commercial seedling supply.
Owner:SOUTHWEST FORESTRY UNIVERSITY

New species of brevibacterium root rot resistance of Huanglonglong and application thereof

The invention discloses a novel brevibacterium root rot resistant species of Huanglonglong and application of the novel brevibacterium root rot resistant species. The invention provides a Huanglonglong brachybacterium root rot resistant strain, which is a Huanglonglong brachybacterium root rot resistant strain, the strain number of the Huanglonglong brachybacterium root rot resistant strain is BS409, and the registration number of the Huanglonglong brachybacterium root rot resistant strain in the China General Microbiological Culture Collection Center is CGMCC No.34201. The invention further provides a preparation method of the Huanglonglong brachybacterium root rot resistant strain. The strain BS409 provided by the invention is a new species of brevibacterium, can generate chitinase and hydrolyze chitin, and has inhibitory activity on root rot pathogenic bacteria fusarium equiseti (Fusarium equiseti). The results indicate that the strain BS409 has good potential in the aspect of biological prevention and control of root rot of medicinal plants (such as bighead atractylodes rhizome, pseudo-ginseng and ginseng), and can also be used in the aspects of soil remediation, soil improvement and the like of planting fields of bighead atractylodes rhizome, pseudo-ginseng and ginseng.
Owner:YANCHENG LIMIN CHEM

Seedling breeding and under-forest cultivation method for Schefflera octophylla

The invention discloses a seedling breeding and under-forest cultivation method for Schefflera octophylla. Relates to the technical field of medicinal plant cultivation. Comprising the following steps: seed treatment; sowing and germinating; seedling cultivation; performing under-forest field planting; and managing after planting. The germination rate is high and neat; seedlings are robust and strong in stress resistance; the field planting survival rate is obviously improved, and the under-forest field planting survival rate is stabilized to be 85% or above; the whole technical route does not need to use chemical pesticides and chemical fertilizers, the Yunnan pine forest land resources are utilized, bionic cultivation of the stichopus japonicus is achieved, the ecological environment is protected, and technical support is provided for production of high-quality medicinal materials of the stichopus japonicus.
Owner:LIJIANG FORESTRY SCI INST (LIJIANG FORESTRY SCI & TECH PROMOTION STATION)

Culture medium for inhibiting browning of paris polyphylla explants

PendingCN121080385APlant tissue cultureHorticulture methodsParis polyphylla var. yunnanensisCell membrane
The invention belongs to the technical field of medicinal plant tissue culture, and particularly discloses a culture medium for inhibiting browning of paris polyphylla explants, and the culture medium is prepared from the following components: 450 to 1300 mg / L of KNO3, 350 to 1220 mg / L of NH4NO3, 50 to 240 mg / L of KH2PO4, 135 to 560 mg / L of MgSO4. 7H2O, 220 to 920 mg / L of CaCl2. 2H2O, 15.2 to 30.5 mg / L of MnSO4. 4H2O, 500 to 5000 mg / L of sodium bentonite and the like. The phyllosilicate structure is utilized to efficiently adsorb phenolic substances, and the enzymatic browning chain reaction is blocked; meanwhile, high calcium salt concentration is maintained to enhance the stability of cell membranes and inhibit exosmosis of phenolic substances. By adopting the culture medium disclosed by the invention, browning can be effectively inhibited, the seedling quality and the production efficiency can be remarkably improved, the seedling culture period and the seedling culture cost can be greatly reduced in the rapid propagation culture process of the paris polyphylla seedlings, and a technical guarantee is provided for commercial seedling supply.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Production method of medicinal plant additive for suckling pig feed containing vitamin B12

The present invention discloses a method containing VB 12 The production method of the medicinal plant additive for suckling pig feed and the product thereof relate to the field of suckling pig breeding. The production method of the medicinal plant additive for suckling pig feed of the present invention comprises the following steps: (1) crushing cardamom, tricholoma, oregano and inulin respectively and enzymolyzing them with cellulase, and then mixing them in proportion, or first mixing cardamom, tricholoma, oregano and inulin in proportion, and then crushing and enzymolyzing them with cellulase to obtain a primary enzymolysis material; (2) adding potassium dihydrogen phosphate and oxalic acid to the primary enzymolysis material, and continuing to degrade to obtain a hot-cooked enzymolysis material; (3) mixing the hot-cooked enzymolysis material with molasses, wormwood, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material; (4) adding pretreated Sanguisorba officinalis and molasses to the primary fermentation material, and continuing to ferment. The medicinal plant additive for suckling pig feed of the present invention can significantly reduce the feed-to-meat ratio of suckling pigs and significantly increase daily weight gain.
Owner:AGRICHINA HUAWEI BIOPHARMACEUTICAL HUBEI CO LTD

Method of obtaining a shelter from platyacantha schrenk (rosa platyacantha schrenk)

UndeterminedKZ12524UBiotechnologyAlcohol
The abstract relates to the field of pharmacy, in particular, to a method for obtaining extracts from medicinal plants and can be used for the production of biologically active additives, cosmetics and medicines with antioxidant, rejuvenating and anti-inflammatory effects. The essence of the utility model is the development of a technology for obtaining a liquid extract rich in biologically active compounds by ultrasonic processing of Rosa platyacantha Schrenk leaf raw materials. The method includes the following steps: 1. Preparation of plant raw materials (grinding to 5 mm); 2. Preparation of extractant (50% ethyl alcohol); 3. Extraction of raw materials and extractant in a ratio of 1:10 (Elma Elmasonic Easy 30H); 4. Filtration of the resulting liquid extract; 5. Packaging and labeling of the finished product. Extraction is carried out at a temperature of 35°C and a frequency of 37 kHz for 30 minutes. The technical result of the proposed approach is the development of a resource-saving technology for obtaining a liquid extract from the leaves of Rosa platyacantha Schrenk, ensuring a reduction in extraction time, a low temperature regime while preserving thermolabile biologically active substances, and an expansion of the raw material base through the use of previously unused parts of the plant.
Owner:NON PROFIT JOINT CO KAZAKH NAT MEDICAL UNIV NAMED AFTER S D ASFENDIYAROV

An armillaria contact type induction device and control system for improving the inoculation rate of gastrodia elata seed germination

PendingCN122296194ABiotechnologyControl system
This invention discloses a *Armillaria mellea* contact induction device and control system for improving the inoculation rate after *Gastrodia elata* seed germination. It relates to the field of co-cultivation technology of edible and medicinal fungi and medicinal plants, and includes a shell, a germination support module, a mycelial induction module, a contact bridge module, a nutrient pulse module, an environmental modulation module, a dual-evidence detection module, and a main control module. The main control module generates an inoculation window index and, after trial contact, switches between a maintained contact state and a withdrawal state based on the recovery curve to complete the contact induction and colonization maintenance of *Armillaria mellea* with *Gastrodia elata* protocorms.
Owner:昭通学院

A method for extracting tripterygium wilfordii quinone A from the root of jasmine

The present invention relates to the technical field of extracting active ingredients from medicinal plants. Specifically, a method for separating and extracting tripterygium wilfordii quinone A from the root of the Chinese herb "Torreya grandis" is disclosed. The method uses the root of the Chinese herb "Torreya grandis" as the raw material and obtains tripterygium wilfordii quinone A monomer through pulverization, reflux extraction, extraction, reverse-phase medium-pressure C18 column chromatography, and reverse-phase high-pressure C18 column chromatography. The method is simple, rapid, and has high socioeconomic value. The obtained tripterygium wilfordii quinone A has a high purity (99.72%), laying the foundation for subsequent research on its pharmacological activity and the development of new immunotherapy drugs.
Owner:CHONGQING YAOYANYUAN PHARM CO LTD

SNP site related to content and proportion of ginsenoside Rg1 and Rb1 in panax notoginseng, combination and application of KASP marker

The application discloses a SNP site and KASP marker combination related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and application thereof, and belongs to the technical field of medicinal plant molecular breeding and quality evaluation. In view of the problems of lack of molecular markers synchronously related to contents and proportions of ginsenosides Rg1 and Rb1 in Panax notoginseng and long breeding cycle in the prior art, nine SNP site combinations located on a Chr11 chromosome of Panax notoginseng genome are screened, and a KASP marker combination and primer combination are developed based on four sites of SNP3-SNP6 among them. The KASP marker combination has strong specificity, is convenient to detect, has high throughput, is low in cost, and can accurately identify the contents and proportions of Rg1 and Rb1 in Panax notoginseng samples. The application can be used for Panax notoginseng germplasm resource screening and molecular marker assisted breeding, early identification of target traits is realized, the breeding cycle is shortened, the breeding efficiency is improved, and technical support is provided for directional cultivation of Panax notoginseng high-quality varieties.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Edible and medicinal plant low-temperature enzymatic complex beverage and preparation method thereof

PendingCN122320142ABiotechnologyAmylase
This invention discloses a low-temperature enzymatic hydrolysis compound beverage made from edible and medicinal plants, and its preparation method. Using jujube powder as a base, at least two kinds of dried powders from medicinal and edible plants are compounded. Enzymatic hydrolysis is performed using a compound enzyme containing cellulase, pectinase, and acidic protease at 45-50℃ and pH 4.0-5.0. The product is then subjected to enzyme inactivation and solid-liquid separation (filtration / centrifugation or a combination thereof). Amylase can be added for high-starch / polysaccharide raw materials. The product contains no added flavorings, colorings, sweeteners, or preservatives. After 12 months of storage at room temperature, the sedimentation rate is ≤0.5%, and the retention rate of active ingredients is 26%-31% higher than that of traditional high-temperature processes. The process is highly versatile and can be adapted to different separation methods, enabling the industrial production of clean-label, highly stable plant-based beverages.
Owner:李彤

Industrialized seedling raising method for shortening growth period of scutellaria baicalensis

The invention relates to the technical field of medicinal plant seedling culture, in particular to an industrialized seedling culture method for shortening the growth period of scutellaria baicalensis, which adopts a multi-layer seedling culture rack integrated sensor network, a tidal irrigation system, a vermiculite and perlite matrix recycling technology and a digital management platform to reduce the matrix cost and improve the space utilization rate. Cultivation environment parameters and water and fertilizer supply are dynamically regulated and controlled, and three-dimensional, precise and efficient scutellaria baicalensis seedling culture is achieved. According to the method, the scutellaria baicalensis seedling raising period is remarkably shortened to 1 / 2 of that of a traditional method, the seedling survival rate is increased to 85% or above, common diseases and pests in traditional seedling raising are reduced, meanwhile, 80% of planting space is saved, and the method is suitable for large-scale production.
Owner:远大种业(甘肃)有限公司

Application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs

ActiveCN121005799Bachieve separationSolve the technical problems of extraction and separationOrganic active ingredientsNervous disorderCelluloseMonosaccharide composition
This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE

Medicinal plant genome assembly method and system based on high-throughput sequencing

The invention relates to the technical field of genome assembly, in particular to a medicinal plant genome assembly method and system based on high-throughput sequencing. The method comprises the following steps: acquiring a target sequencing sequence, and screening a function influence sequence; performing repetitive classification on the target sequencing sequence to obtain a non-repetitive sequence cluster and a repetitive sequence cluster, and dividing the repetitive sequence cluster into a functional repetitive sequence and a non-functional repetitive sequence; determining dynamic association strength by combining expression abundance of functional genes, content detection of medicinal components and base overlapping characteristics of adjacent sequences; constructing an association network; and according to the dynamic association strength in the association network, aiming at the functional association type repetitive sequence, the non-functional association type repetitive sequence and the adjacent non-repetitive sequence, respectively carrying out sequence splicing by adopting a differential assembly path to obtain a complete genome assembly result. According to the method, the problems of splicing errors, fragment breakage and redundancy caused by repeated sequences can be solved, and the assembly accuracy and integrity are remarkably improved.
Owner:XIAN BOTANICAL GARDEN SHAANXI PROV

Medicinal lotus cell suspension factory system and method for directionally producing high-activity components based on healthy target spots

The invention discloses a medicine lotus cell suspension factory system and method for directionally producing high-activity components based on a healthy target spot, and the system is characterized in that a health-oriented cell line design module, an intelligent scale culture module, a precise metabolism induction module and a targeted separation and product development module are in closed-loop linkage; and an innovative mode of reversely designing a production process from health requirements is realized. According to the method, aiming at specific targets of epidemic resistance, depression resistance, reproductive health and the like, a high-yield cell line is created through multi-omics analysis screening or gene editing, and large-scale culture is performed in an optimally controlled bioreactor; and the elicitor is utilized to accurately stimulate a metabolic pathway to directionally enrich high-value components such as liensinine, nuciferine, epigallocatechin gallate and the like. And finally, preparing a standardized extract by adopting a green extraction technology (such as ultrasonic extraction and supercritical CO2 extraction), wherein the standardized extract is used for functional foods, dietary supplements or medical products.
Owner:HUBEI ZHONGLIAN WETLAND RESEARCH INSTITUTE CO LTD

Method for increasing content of sesquiterpene glycoside in dendrobium nobile

The invention belongs to the technical field of medicinal plant cultivation, and particularly provides a method for increasing the content of sesquiterpene glycoside in dendrobium nobile. The method comprises the step of applying a magnesium ion-containing solution with effective concentration at the growth stage of dendrobium nobile. Preferably, the magnesium ion-containing solution is a magnesium chloride solution, the application concentration is 10-500 mg / L, the magnesium ion-containing solution is applied in a foliage spraying or root irrigation mode, and the key period of application is the rapid growth period or the effective component accumulation period of the dendrobium nobile. The promotion effect of exogenous magnesium ions on biosynthesis of the sesquiterpene glycoside of the dendrobium nobile is disclosed for the first time, and the content of sesquiterpene glycoside components in the dendrobium nobile medicinal material can be remarkably increased under the condition that a basic cultivation mode is not changed, so that the medicinal potential and market value of immunoregulation, anti-inflammation and the like of the dendrobium nobile medicinal material are enhanced. The method is simple, efficient, low in cost, free of pollution and suitable for industrial application.
Owner:ZUNYI MEDICAL UNIVERSITY