87 results about "Clinacanthus nutans" patented technology

The invention relates to a series Clinacanthus nutans tea beverage and a preparation method thereof, belongs to the technical field of food processing, and aims to solve the problems of flocculation precipitation of the Clinacanthus nutans tea beverage, color changes of tea soup and bad smell of the tea soup. The series Clinacanthus nutans tea beverage disclosed by the invention relates to the series of a primary taste tea beverage, a fruit juice tea beverage, a milk tea beverage, a composite tea beverage and the like. The preparation method comprises the following steps of firstly, performing fixation on the Clinacanthus nutans leaves, baking the Clinacanthus nutans leaves after fixation, and stir-frying the baked Clinacanthus nutans leaves so as to obtain the Clinacanthus nutans tea; and mixing the Clinacanthus nutans tea and purified water so as to obtain a mixture, adding beta-cyclodextrin, preparing aqueous extract liquid, then performing enzymolysis treatment, and then adding vitamin C, citric acid, a stabilizing agent and seasoning, performing homogenizing treatment, and then performing disinfection so as to obtain the series Clinacanthus nutans tea beverage. The series Clinacanthus nutans tea beverage disclosed by the invention can still keep clear and transparent after being preserved for 12 months at normal atmospheric temperature after disinfection, flocculent precipitation and a supernatant do not exist, the smell has delicate fragrance, the series Clinacanthus nutans tea beverage has the flavor characteristic of the tea beverage, and the and organoleptic investigation result is good.

The invention relates to a series Clinacanthus nutans functional beverage and a preparation method thereof, belongs to the technical field of food processing, and aims to solve the problems of flocculation precipitation of the Clinacanthus nutans functional beverage, color changes of tea soup and bad smell of the tea soup. The series Clinacanthus nutans functional beverage disclosed by the invention comprises Clinacanthus nutans aqueous extract enzymatic hydrolysate, vitamin C and varied minerals, and plant water solubility extracts or active substances can also be added. The preparation method comprises the following steps of firstly, preparing Clinacanthus nutans powder, compounding a Clinacanthus nutans aqueous solution, adding beta-cyclodextrin, adjusting the pH of the systems, then performing enzymolysis treatment, then adding various active substances, performing homogenizing treatment, and then performing disinfection so as to obtain the series Clinacanthus nutans functional beverage. The prepared series Clinacanthus nutans functional beverage disclosed by the invention can still keep clear and transparent after being preserved for 9 months at normal atmospheric temperature after disinfection, the smell has delicate fragrance, and the series Clinacanthus nutans functional beverage can have the effects of regulating the balance of body fluid and improving hypoxia resistance.

The invention provides an extracting method of a clinacanthus nutans anti-cancer active part. The extracting method is as follows: refluxing and extracting a clinacanthus nutans medicinal material by hot water, and then obtaining clinacanthus nutans polysaccharide by alcohol-precipitating, centrifuging and separating; eluting the centrifuged liquid supernatant by macroporous adsorption resin and 80%-95%(v / v) alcohol to obtain a water-soluble part; refluxing and extracting the clinacanthus nutans medicinal material by 50%-80% (v / v) alcohol, extracting water-containing suspension liquid of alcohol extract by petroleum ether, dichloromethane and ethyl acetate in a fractional manner to obtain an ethyl acetate active part, and extracting residual water-phase extracting liquor by n-butyl alcohol, and evaporating out the solvent to obtain a n-butyl alcohol part. The clinacanthus nutans anti-cancer active part disclosed by the invention is at least one of the clinacanthus nutans polysaccharide, the water-soluble part, the ethyl acetate part and the n-butyl alcohol part, can be used for preparing an anti-tumor drug, and has characteristics of being high in life rate of elongation, and small in toxic or side effect.

The invention provides a traditional Chinese medicinal mixture for preventing fatty liver and a preparation method and application thereof. The preparation method comprises the following steps of mixing 5-15 parts of salvia miltiorrhiza, 3-5 parts of garlicin and 3-5 parts of crataegus pinnatifida, soaking in 20-30 parts of water for 1 hour, decocting at 180-200DEG C for 30-60 minutes, filtering, adding 10-20 parts of water to filter residue, decocting at 180-200DEG C for 30-60 minutes, filtering, mixing decoctions, adding 10-13 parts of clinacanthus nutans powder of 20 meshes, reflux-extracting at 100DEG C twice (3 hours each time), concentrating the extractive solution under reduced pressure, adding anhydrous ethanol, precipitating overnight at 4-20 DEG C, collecting filtrate, and concentrating at 70-80DEG C to 1 / 3 to 1 / 4 of the original volume to obtain the traditional Chinese medicinal mixture for preventing fatty liver. The traditional Chinese medicinal mixture for preventing fatty liver can be administered as liquid, capsules, tablets and other dosage forms, or administered by water decoction with teal leaves to prevent fatty liver by virtue of everyday life habits of people. The traditional Chinese medicinal mixture is convenient and has a good effect.

The invention provides a preparation method of high-purity paclitaxel. The preparation method comprises the following steps: (1) establishing a stable taxus chinensis forming layer stem cell culture system; (2) adding taxus chinensis endophytic fungi extract liquid into a suspension culture medium, inoculating taxus chinensis cells, performing the ventilation culture for 6 to 8 days under an artificial lighting condition, and adding jasmonic acid methyl ester and a clinacanthus nutans extract into the liquid suspension culture medium to continue the culture; (3) collecting the taxus chinensiscells, and performing the coarse extraction of paclitaxel; (4) purifying a crude extract; and (5) enabling a concentrated solution II to pass through a C18 high-performance liquid phase chromatographic purification column, eluting by using methanol, collecting and combining paclitaxel elution parts, decompression evaporating the solvent methanol, crystallizing and releasing the paclitaxel, centrifugally collecting crystals, collecting and combining the paclitaxel elution part, decompression evaporating the solvent methanol to crystallize and precipitate the paclitaxel, centrifugally collectingthe crystals, and vacuum freeze drying the crystals, thus obtaining the high-purity paclitaxel concentrated solution II. The preparation method has the characteristics of high induction success rate,high paclitaxel extraction purity and the like.

The invention discloses a preparation method for clinacanthus nutans extract. The preparation method specifically comprises the following steps: drying, smashing and sieving the plant part of the clinacanthus nutans, extracting by using ethanol, decompressing to obtain ethanol coarse extract by using an ethanol recycling agent, adding water to the ethanol extract and diluting, extracting by usingan organic solvent, decompressing and concentrating extract liquid to obtain the extract, vacuum-drying the extract to obtain powder which is the clinacanthus nutans extract namely. The extract has the remarkable inhibiting effect to human leukemia, liver cancer, lung cancer, breast cancer and colon cancer cells through an in-vitro cytotoxic activity test, and has the better anti-tumor activity, and can be used for preparing an anti-tumor drug.

The invention discloses a clinacanthus nutans functional tea beverage formulation and a preparation method thereof and relates to the technical field of functional tea preparation. The formulation iscomposed of, by weight, clinacanthus nutans, sweetener, water, enzyme, vitamin C, osmanthus fragrans, rose, flos chrysanthemi, saffron, fructus lycii extract, fructus ziziphi jujubae extract, fig extract, radix glycyrrhizae extract, large-fruited Chinese hawthorn extract and green tea. After adopting the above technical scheme, the formulation has the advantages that the formulation contains various natural raw materials, thereby being capable of meeting needs of modern people on beverages capable of moistening throat to relieve thirst and having nutritional and healthcare functions; the formulation has fragrant smell, typical beverage flavor and multiple flavor options, and market needs are expanded; the preparation method meets needs of industrial production, and the formulation has widemarket prospect.

The invention provides a feed for improving the immunity of a black muscovy duck and a preparation method thereof. The feed is obtained by grinding and mixing the following raw materials: 100-120 parts of wheat brans, 80-100 parts of bean cake, 10-20 parts of lysine, 5-10 parts of tryptophan, 5-10 parts of methionine, 5-10 parts of Chinese angelica roots, 10-20 parts of clinacanthus nutans, 1-2 parts of bacillus, 1-2 parts of lactic acid bacteria, 1-2 parts of vitamin A, 1-2 parts of vitamin E and 1-2 parts of vitamin C. According to the feed, amino acid, Chinese angelica roots, clinacanthus nutans, bacillus, lactic acid bacteria and vitamins are added into daily ration of the black muscovy duck, the different requirements on the nutrition of the black muscovy duck during different growth and physiology periods can be met, meanwhile, the feed nutrition is balanced in an emphasis manner, the quality of the black muscovy duck can be effectively improved, and the immunity is improved, so that the feeding cost is lowered, and the culture benefits are improved.

The invention discloses a method for extracting clinacanthus nutans active substances through ultrasonic assistance. The method mainly comprises the steps that 1, raw materials are cleaned up, drained, dried, ground through a superfine grinding mill and screened with a 200-mesh sieve, and then clinacanthus nutans powder is obtained; 2, after being subpackaged, the clinacanthus nutans powder is placed into a refrigerator and preserved at 5 DEG C for standby application; 3, the ultrasonic power, ultrasonic temperature and ultrasonic time of an ultrasonic cleaner are regulated; 4, a certain amount of the clinacanthus nutans powder is accurately weighed, and an appropriate amount of water is added according to the material-liquid ratio; 5, the clinacanthus nutans powder is put into ultrasonic waves of which the ultrasonic conditions are regulated in advance for ultrasonic treatment, wherein the conditions are set as followed: the ultrasonic time is set for 10-50 min, the ultrasonic temperature is set at 30 DEG C-70 DEG C, the material-liquid ratio is set to be 1:20-1:60, and the ultrasonic power is set at 72-216 W; 6, the solution processed through the ultrasonic waves are centrifuged at 6000 r / min for 15 min, and a supernatant solution, that is, a clinacanthus nutans active substance extracting solution is obtained.

The invention discloses a Chinese herbal medicine gargle for nursing after oral surgery. The Chinese herbal medicine gargle is prepared from the raw materials in parts by weight: 5-15 parts of clinacanthus nutans, 4-12 parts of blumea balsamifera, 8-10 parts of Chinese fevervine herb, 7-10 parts of radix ophiopogonis, 7-9 parts of wedelia chinensis, 6-9 parts of callicarpa nudiflora, 1-5 parts oflivistona chinensis, 1-5 parts of green tea, 10-15 parts of benincasa hispida, and 50-75 parts of water. By adopting the Chinese herbal medicine gargle for nursing after the oral surgery, the effective ingredients of formula Chinese herbal medicine are contained, and thus the effect of relieving swelling and pain can be achieved; benincasa hispida tea water is added, on the one hand, the smell ofChinese herbal medicine is adsorbed, the refreshed and fragrant effects are achieved, on the other hand, the content of botanical glycosides is increased, and the taste is improved; and the Chinese herbal medicine gargle is used for nursing after the oral surgery and has the obviously good prevention and treatment effects on complications of inflammation and pain, nausea, dry mouth and bitter taste, oral ulcer, gingival hemorrhage and the like.

The invention discloses a tissue culture and propagation method for clinacanthus nutans. The method can acquire a large number of clinacanthus nutans cultivating seedlings in a short term and achieveeffective protection and propagation of the clinacanthus nutans. The method is simple in operation and easy to carry out, the used culture medium and reagent are easy to prepare, the cost is low, andthe method can be popularized in a wide range.

The invention discloses clinacanthus nutans herbal granulated tea and a preparation method. The clinacanthus nutans herbal granulated tea is prepared from, by weight, 20-85 parts of clinacanthus nutans, 10-30 parts of herb and 15-45 parts of fruit and vegetable peel, wherein the herb comprises rosemary, balm, Basil leaves, thyme and stevia rebaudiana. The fruit and vegetable peel comprises watermelon peel, exocarpium benincasae, bitter gourd peel, momordica grosvenori fruit peel and orange peel. The same cool attribute is selected, scientific blending is carried out, a synergic effect is achieved, defects are removed, advantages are reserved, cleaning and supplementing are achieved together, and the function of improving immunity is achieved. No preservative or liquid glucose is added, thesafety of the product is improved, nutritional ingredients are easy to absorb, and the sensory flavor is good. According to the preparation method, a specified submicron powder process, mixing process, blending process, extrusion forming process and the like are adopted, granular particles are bonded through self adhesive force, and an excellent process is achieved.

The invention provides a traditional Chinese veterinary medicine premix and a preparation method thereof. The traditional Chinese veterinary medicine premix comprises the following raw materials in parts by weight: 10 to 20 parts of lucid ganoderma, 30 to 40 parts of clinacanthus nutans, 10 to 20 parts of seaweed, 10 to 20 parts of radix morindae officinalis, 10 to 20 parts of betel nuts, 1 to 6 parts of probiotics and 2 to 8 parts of an auxiliary material. The traditional Chinese veterinary medicine premix disclosed by the invention can be used for treating or preventing swine viral infectious diseases like swine fever, African swine fever and highly pathogenic porcine reproductive and respiratory syndrome, and solves the important problems of the pig industry.

The invention provides a traditional Chinese medicine mixture capable of delaying skin ageing, a preparation method thereof and applications thereof. The traditional Chinese medicine mixture is prepared by decocting 15-50 parts of pilose asiabell root, 20-30 parts of astragalus root and 10-20 parts of poria cocos to obtain a decoction liquid, mixing the decoction liquid with 5-10 parts of clinacanthus nutans powder, extracting with water by refluxing, subjecting obtained extract to concentration under vacuum, layering with ethanol to obtain the filtrate, subjecting 5-10 parts of donkey-hide gelatin to enzymolysis by an animal proteolytic enzyme to obtain a donkey-hide gelatin raw material, and finally combining the filtrate and the donkey-hide gelatin raw material. By studying reasonable proportion and synergistic effects of the medicine material components, the traditional Chinese medicine mixture has good curative effects in the field of delaying skin ageing.

The invention provides a processing technology for preparing coconut milk by a whole coconut meat wet crushing method. The processing technology comprises the following steps that S1, fresh and maturecoconut meat is taken and soaked in a sodium chloride solution for 8-12 minutes, the coconut meat is washed with water, and crushed to obtain crushed coconut meat; S2, the crushed coconut meat, nonienzyme and coconut water are mixed, and low-temperature fermentation is carried out to obtain a coconut fermentation product; S3, the coconut fermentation product is added into a sodium carboxymethylcellulose aqueous solution for mixing, coarse grinding is carried out for 4-8 minutes in a wet-method superfine fine grinding machine under the conditions that the coarse grinding frequency is set tobe 20-40 Hz, the rotating speed is 3000-8000 rpm and a grinding disc is 0.3-0.6 mm, and fine grinding is carried out for 2-3 minutes under the conditions that the fine grinding frequency is set to be30-50 Hz, the rotating speed is 9000-12000 rpm and the grinding disc is 0.08-0.12 mm to obtain ground pulp; and S4, the ground pulp is mixed with an annona squamosa extract, a clinacanthus nutans extract and trehalose, uniform stirring is carried out, and after homogenizing is carried out twice, sterilization and vacuum concentration are carried out to obtain the coconut milk. The coconut milk prepared by the processing technology is good in overall stability and has relatively high oxidation resistance and antibacterial property.

The invention discloses a double tetrahydrofuran lignin, a preparation method and an application thereof. The preparation of the lignin comprises the following steps: (1) crushing clinacanthus nutans and then soaking in ethyl alcohol, refluxing and extracting, thereby acquiring total ethyl alcohol extract, and then uniformly dispersing in distilled water and using petroleum ether and ethyl acetate for extracting in turn; (2) treating the petroleum ether part extract with silica gelcolumn chromatography, adopting a petroleum ether-ethyl acetate solvent system for gradient eluting, collecting, concentrating and mixing the same flow components; (3) performing reverse phase silica gel column chromatography separation, Sephadex LH-20 sephadex column chromatography separation and high performance liquid chromatography separation on the flow component acquired by eluting with petroleum ether and ethyl acetate at ratio of 5:1, thereby acquiring the double tetrahydrofuran lignin. According to the invention, the double tetrahydrofuran lignin extracted from clinacanthus nutans has inhibitory activity to cervical cancer Hela cells, breast cancer MCF-7 cells and lung adenocarcinoma A549 cells.

The invention discloses a traditional Chinese medicinal composition for treating primary dysmenorrhea. According to the principle of traditional Chinese medicine, the pathogenesis of primary dysmenorrhea is that due to pathogenic factors in menstrual period, Chong and Ren channels are obstructed or pathogenic cold accumulates in channels, qi and blood circulation is not smooth, and menstrual blood circulation in uterus is obstructed, thus causing pain; or Chong and Ren channels and uterus are not nourished, thus causing pain. Traditional Chinese medicinal materials with effects of promoting blood circulation, dispelling blood stasis, promoting qi circulation, dispelling cold and relieving pain, including Rosa banksiae, Ophiorrhiza japonica, Stylophorum lasiocarpum, Tetrastigma obtectum, Murraya exotica, Clinacanthus nutans, Radix mandragorae caulescentis, Carlesia sinensis, Rosa taiwanensis and Swertia bifolia, are decocted in water to obtain decoction, and according to the clinical trials, the total effective rate is up to 96.7%, and the therapeutic effect is remarkable.

The invention discloses a traditional Chinese medicine patching agent for treating hyperostosis. The hyperostosis belongs to the range of paralysis and heumatism according to clinical manifestation and onset age. According to inducements of wind, cold, dampness, heat and strain, loaded inflammatory pathological changes of bones are caused, so that disease symptoms such as qi and blood loss, blood stasis and stagnation and unsmooth main and collateral channels are caused. The medicine is prepared by the following steps: selecting traditional Chinese medicines such as eclipta, diversifolious osmanthus branchlet leaf and bark, Clinacanthus nutans, fragile blueberry root, pyrola, Yunnan begonia herb, scabrous doellingeria root, pedunculate acronychia wood or root, Centranthera cochinchinensis, red psychotria root and Japanese pagodatree bark, with the effects of warming liver and kidney, activating blood circulation and dispersing blood stasis, relaxing muscles and tendons and removing obstruction from meridians, softening bony spur and eliminating cold and dampness, adding vinegar, distilled spirit and water, thereby obtaining the patching agent. According to the clinical test in the institute, the total effective rate is 97.4 percent, and the curative effect is good.

The invention provides a fodder capable of improving the egg laying capacity of black muscovy ducks and a preparing method of the fodder. The fodder is obtained by grinding and mixing raw materials which comprise 100-120 parts of wheat bran, 80-100 parts of bean cake, 10-20 parts of bone meal, 5-10 parts of edible salt, 5-10 parts of chicken powder, 5-10 parts of angelica sinensis, 10-20 parts of clinacanthus nutans, 1-2 parts of bacillus and 1-2 parts of lactic acid bacterium. According to the fodder capable of improving the egg laying capacity of the black muscovy ducks and the preparing method of the fodder, the mode of adding the bone meal, the edible salt, the chicken powder, the angelica sinensis, the clinacanthus nutans, the bacillus and the lactic acid bacteria in the daily ration of the black muscovy ducks is used for improving the blood circulation of the black muscovy ducks, delaying senescence and improving reproductive performance and immunity, so that the egg laying capacity of the black muscovy ducks and the nutritive value and the taste of duck eggs are improved, the eliminating time of the black muscovy ducks is delayed, the utilization years of female muscovy ducks is prolonged, and thus the feeding cost is reduced, and the breeding benefits are improved.

The invention discloses a traditional Chinese medicinal composition containing clinacanthus nutans and a preparation method thereof, and belongs to the technical field of medicines or health foods. The traditional Chinese medicinal composition comprises the following components in parts by mass: 10-15 parts of clinacanthus nutans, 2-4 parts of Chinese date, and 1-2 parts of fresh ginger. The preparation method comprises the following preparation steps: A, respectively cleanly washing the clinacanthus nutans, the Chinese date, and the fresh ginger, and performing impurity removal; B, mixing theChinese date and the fresh ginger with appropriate water and performing boiling, and performing filtering to obtain a boiled liquid; C, performing alcohol extraction of the clinacanthus nutans to obtain an extraction liquid; D, concentrating the extraction liquid to obtain a concentrated liquid; E, uniformly mixing the boiled liquid and the concentrated liquid to obtain a mixed liquid; and F, mixing the mixed liquid with an appropriate amount of maltodextrin, and completing granulation. Problems generally existing in clinacanthus nutans products at present such as high sugar content, low effective content and poor effects can be effectively solved.

The invention discloses a preparation method of honeysuckle flower compound tea. The preparation method comprises the following steps: carrying out pile fermentation on primary auxiliary materials including folium perillae, clinacanthus nutans herb, herba andrographitis and lophatherum herb; then steaming the fermented primary auxiliary materials and secondary auxiliary materials including radix platycodonis, radix isatidis and fructus lycii to obtain a steaming solution; after steaming, airing and baking the secondary auxiliary materials; adding honeysuckle flowers and honey into the steaming solution and immersing; fishing out the immersed honeysuckle flowers, and airing and baking; finally, mixing the backed secondary auxiliary materials and the baked honeysuckle flowers to obtain the honeysuckle flower compound tea. The preparation method disclosed by the invention is simple; the honeysuckle flower compound tea has a better outward appearance and lasting and rich aroma; after the honeysuckle flower compound tea is brewed, soup is bright green, glittering and translucent; the taste is better and the pharmacological health-care effect is obvious; the honeysuckle flower compound tea is suitable for storage and has a moth-proof effect, and does not need to be additionally smoked by sulfur so that the honeysuckle flower compound tea meet quality standard requirements of green foods.

The invention relates to the technical field of probiotic fermentation, in particular to enzyme liquid, application and a preparation method thereof, and an anti-aging product. The enzyme liquid is prepared from more than 40 raw materials such as coconut water, passion fruits, pineapples, fructus alpiniae oxyphyllae, clinacanthus nutans and opuntia ficus-indica. According to the invention, variousvegetables, fruits and herbaceous plants are selected as raw materials to prepare enzyme liquid, wherein the tropical fruits in the formula of the product not only have good taste, but also have multiple health-care effects; the enzyme beverage prepared by compounding clinacanthus nutans, fructus alpiniae oxyphyllae and the like with the anti-aging effect can generate rich nutritional ingredientssuch as enzymes, vitamins, minerals, amino acids, various organic acids and polysaccharides; therefore, antioxidant components such as alpinia oxyphylla can be completely released, new small molecules are generated, growth of probiotics in the intestinal tract is promoted, free radicals in the body are removed in time, senile dementia is prevented, and aging is resisted.

The invention discloses traditional Chinese medicine powder for treating postoperative upper limb edema of a breast cancer. Traditional Chinese medicine believes the disease is caused by injuried blood vessels and nerves to result in unsmooth qi and blood circulation, subcutaneous water flow, blood stasis and internal retention, a patient has insufficient vital qi originally, and vitality is injured by postoperative radiotherapy and chemotherapy, and the patient is incapable of promoting blood circulation to result in the blood stasis, phlegm dampness and retention, thereby causing the disease. Accordingly, the traditional Chinese medicine powder adopts begonia fimbristipula, clinacanthus nutans, a discadenoid cherry root, gynura divaricata, a dichrocephala root, clerodendrum indicum, splendid achnatherum, desmodium heterocarpon, flemingia macrophylla, a radix fici simplicissimae root, railway beggarticks herb, and primrose willow which are ground, bagged and heated for external application. Clinical trials show the powder has a total effective rate up to 93.33%, and a significant curative effect.

The invention provides an asparagus anti-haze beverage and a preparation method thereof. Every 1,000 parts by weight of the asparagus anti-haze beverage is prepared from, by mass, 80-120 parts of asparagus, 40-80 parts of radix ophiopogonis, 40-80 parts of radix polygonati officinalis and 10-50 parts of clinacanthus nutans and further comprises 30-70 parts of honey. The preparation method includes the steps that A, the raw materials are selected and cleaned; B, the raw material components of asparagus, radix ophiopogonis, radix polygonati officinalis and clinacanthus nutans are extracted with water, a water extracting solution is concentrated to obtain thick paste, the honey and other accessories are added into the thick paste, and the finished product is prepared. The beverage is a drinking product with smooth and refreshing taste, slightly fragrant smell and the clear color and luster, has the functions of clearing away the lung-heat and relieving the sore-throat, and can effectively relieve symptoms such as dry coughs, throat dryness and pain and pasty taste, and due to the fact that the Hunan Yuan River asparagus serves as one of the raw materials, the good anti-haze effect is achieved.

The invention provides a method for producing paclitaxel by using Chinese yew cambium stem cells, comprising the following steps: 1)establishing a stable Chinese yew cambium stem cell culture system;2) adding a Chinese yew endophytic fungus extract solution into a suspension culture medium, inoculating Chinese yew cells, performing aerobic culture for 6-8 days under the conditions of artificial illumination, and adding methyl jasmonate and clinacanthus nutans extract to a liquid suspension medium to continue culturing; 3) collecting the Chinese yew cells, and performing crude extraction of paclitaxel; 4) purifying the crude extract; 5) adding a tetrahydrofuran-acetone mixed solution to the extract II, heating and dissolving, cold-cutting to room temperature, performing natural crystallization, re-dissolving the crystal into the tetrahydrofuran-acetone mixed solution for recrystallization for 2 to 3 times for obtaining high-purity paclitaxel. The method for producing paclitaxel by using Chinese yew cambium stem cells has the characteristics of high induction success rate and high paclitaxel content in unit cell.

The invention discloses a pharmaceutical composition for treating impotence as well as a preparation method, a preparation and application thereof. The pharmaceutical composition for treating the impotence is prepared from the following raw materials in parts by weight: 100 to 400 parts of radix Crepis rigescens, 100 to 300 parts of herba cistanches, 100 to 200 parts of radix ginseng and 100 to 300 parts of radix Clinacanthus nutans; the pharmaceutical composition is prepared through the steps of pre-treatment, extraction and post-treatment. The preparation is of tablets, powder, capsules, granules and pills which are prepared by adding pharmaceutically acceptable auxiliary materials into the pharmaceutical composition for treating the impotence. The invention provides the application of the pharmaceutical composition for treating the impotence to preparation of medicines for treating the impotence. According to the pharmaceutical composition disclosed by the invention, all the medicines are combined, have a synergistic effect and commonly have the effects of invigorating qi and tonifying kidney and tonifying yang and reinforcing the vital essence; the pharmaceutical composition is used for treating the impotence, soreness and weakness of waist and knees, frequent urination and the like.

The invention discloses a traditional Chinese medicine external application agent for treating postoperative cystospasm. According to the traditional Chinese medicine external application agent, the traditional Chinese medicines namely celastrus orbiculatus, euonymus oxyphyllus, clinacanthus nutans, west china hypericum, radix achyranthis bidentatae, latifolious rush rhizome, perilla stem, angelica polymorpha maxim, alpinia japonica and japanese spicebush bark are selected according to the etiology and pathogenesis of postoperative cystospasm, grinded into fine powders, the various medicine powders are uniformly mixed, blended into a thick paste by mature vinegar, directly apply the umbilical fossea of patients, and covered by a gauze for hot compress; through clinical tests, the traditional Chinese medicine external application agent is remarkable in curative effect, and worthy of clinical popularization and application.

The invention discloses clinacanthus nutans enzyme liquid cataplasm and a preparation method thereof, and belongs to the technical field of cataplasm. The clinacanthus nutans enzyme liquid cataplasm comprises the following components in parts by mass: 40-80 parts of a clinacanthus nutans enzyme liquid, a water-soluble matrix and a protection membrane, wherein the water-soluble matrix comprises the following components: 5-8 parts of sodium polyacrylate, 0.15-0.25 part of dihydroxy aluminum aminoacetate, 1.5-4 parts of ployvinylpynolidone, 35-48 parts of glycerinum, 0.15 part of ceramic powder, 0.01 part of ethylenediamine tetraacetic acid and 0.05 part of citric acid. Compared with the prior art, the clinacanthus nutans enzyme liquid cataplasm has the beneficial effects that as the cataplasm is prepared from clinacanthus nutans, the cataplasm is convenient to use, and remarkable and stable in medicinal effect, the medicinal effect can be greatly improved, on one hand, a wound can be covered to prevent infectious matters, on the other hand, a penetration enhancer can be added for percutaneous drug delivery, and thus bruise swelling can be treated.

The invention discloses clinacanthus nutans anti-cancer liquid, which is prepared through the following steps of (1) picking fresh leaves of clinacanthus nutans, and cleanly cleaning the leaves by clean water; (2) performing cleaning twice by double distilled water; performing freeze-drying; crushing the leaves into fragments being 10 to 20mm; performing heat insulation for 1 to 2h at 50 DEG C; (3) adding distilled water being 100 to 120 times of the volume of the fresh leaf fragments; adding a complex enzyme preparation being 0.02 to 0.05 time of the mass of the fresh leaves for enzymolysis; (4) adding EDTA (ethylene diamine tetraacetic acid) until the final concentration is 0.5mmol / L; adding glycerin until the final concentration is 0.1g / L; adding linalool until the final concentration is thousandth; (5) performing storage at 4 to 8 DEG C. The clinacanthus nutans anti-cancer liquid has the advantages that the preparation method is simple; the inhibition ratio on cancer cells of liver cancer, stomach cancer, breast cancer, lung cancer and the like reaches 50 percent or higher; the taking for a long time is not needed; various cardiovascular and cerebrovascular diseases, and various complications such as hypertension, hyperlipidemia and nephropathy can be effectively controlled; the microcirculation of each organ of the human body is comprehensively conditioned; the metabolic function is improved; no toxic or side effects exist; the clinacanthus nutans anti-cancer liquid can be taken for a long time; the safety is reliable.

The invention discloses a preparation method for a feed for preventing and treating pet obesity. The feed disclosed by the invention is prepared by the following steps: washing 6 to 8 mass parts of clinacanthus nutans and 6 to 8 mass parts of alfalfa for 1 to 3 times in boiled water, and draining, drying and grinding the clinacanthus nutans and the alfalfa into powder; boiling 14 to 20 parts of minced pork with water, making soup, adding clinacanthus nutans powder and alfalfa powder which are obtained in the step (1), 15 to 25 mass parts of corn flour, 13 to 15 mass parts of wheat gluten, 1 to 3 mass parts of chromium yeast and 10 to 15 mass parts of bone powder into the soup, mixing uniformly, boiling and drying the mixture with big fire, allowing standing still for 5 to 10 days in a sterile environment at room temperature, taking the mixture out, and drying and granulating to obtain the feed. The feed disclosed by the invention can provide nutrient substances with enough nutrition, and also has a certain effect on preventing and treating the pet obesity.