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Extracting method of clinacanthus nutans anti-cancer active part and application thereof

A technology of anti-cancer activity and extraction method, which is applied in anti-tumor drugs, medical raw materials derived from angiosperm subphylum, drug combination, etc. It can solve the problem of unclear anti-cancer active parts of crocodile crocodile and unsatisfactory anti-cancer effects, etc. problem, to achieve the effect of less toxic side effects and high life extension rate

Active Publication Date: 2014-06-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an extraction method and application of the anti-cancer active part of Alligator's mouth flower, aiming to solve the problem that the anti-cancer active part of Alligator's mouth flower is not clear and the anti-cancer effect is not ideal

Method used

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  • Extracting method of clinacanthus nutans anti-cancer active part and application thereof
  • Extracting method of clinacanthus nutans anti-cancer active part and application thereof

Examples

Experimental program
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Embodiment 1

[0023] A method for extracting the anticancer active part of crocodile flower, comprising the following steps:

[0024] (1) Take 20 meshes and 60 g of crocodile crocodile powder and extract twice at 100°C with 480 mL of water for 3 hours each time. After concentrating the extract under reduced pressure, add 600 mL of absolute ethanol to precipitate overnight. Centrifuge at 3000 r / min for 20 min, freeze-dry (<-50°C, 0.7 mbar, 14 h) to obtain crocodile polysaccharide (CNPS) from the precipitate; the supernatant is concentrated to 1 / 30 of the original volume and passed through HP-20 Pore ​​adsorption resin, washed with water until colorless, then eluted with 2 BV 95% (v / v) ethanol at a flow rate of 20 mL / min, collected the eluate, concentrated, and freeze-dried to obtain the water-soluble fraction (CN-H);

[0025] (2) Take 20 meshes and 90 g of crocodile flower medicinal powder and add 1080 mL of 80% (v / v) ethanol for reflux extraction, and evaporate the solvent. Disperse the et...

Embodiment 2

[0029] (1) Take 20 meshes and 80 g of crocodile crocodile powder and extract twice with 800 mL of water at 100°C for 3 hours each time.

[0030] Concentrate the extract under reduced pressure, add 1000 mL of absolute ethanol, precipitate overnight, and centrifuge at 3000 r / min for 20 min, and freeze-dry (<-50 °C, 0.7 mbar, 14 h) to obtain the polysaccharide of crocodile flower (CNPS ); the supernatant was concentrated to 1 / 10 of the original volume, passed through HP-20 macroporous adsorption resin, washed with water until colorless, then eluted with 10 BV 80% (v / v) ethanol at a flow rate of 10 mL / min, and collected The eluate was concentrated and freeze-dried to obtain the water-soluble fraction (CN-H);

[0031] (2) Take 20 meshes and 70 g of crocodile flower powder and add 560 mL of 50% (v / v) ethanol to reflux for extraction, and evaporate the solvent. Disperse the ethanol extract of crocodile flower with water, then sequentially use 60 mL×2 petroleum ether, dichloromet...

Embodiment 3

[0033] A method for extracting the anticancer active part of crocodile flower, comprising the following steps:

[0034] (1) Take 20 meshes and 70 g of crocodile crocodile powder and extract twice at 100 °C with 700 mL of water for 3 hours each time. After concentrating the extract under reduced pressure, add 700 mL of absolute ethanol to precipitate overnight. Centrifuge at 3000 r / min for 20 min, and freeze-dry (<-50°C, 0.7 mbar, 14 h) to obtain crocodile polysaccharide (CNPS); the supernatant is concentrated to 1 / 5 of the original volume and then adsorbed by XAD16N macropores The resin was washed with water until it was colorless, and then eluted with 5 BV of 90% (v / v) ethanol at a flow rate of 5 mL / min. The eluate was collected, concentrated, and freeze-dried to obtain the water-soluble fraction (CN-H);

[0035](2) Take 20 meshes and 80 g of crocodile crocodile powder and add 800 mL of 70% (v / v) ethanol to reflux for extraction, and evaporate the solvent. Disperse the ethan...

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Abstract

The invention provides an extracting method of a clinacanthus nutans anti-cancer active part. The extracting method is as follows: refluxing and extracting a clinacanthus nutans medicinal material by hot water, and then obtaining clinacanthus nutans polysaccharide by alcohol-precipitating, centrifuging and separating; eluting the centrifuged liquid supernatant by macroporous adsorption resin and 80%-95%(v / v) alcohol to obtain a water-soluble part; refluxing and extracting the clinacanthus nutans medicinal material by 50%-80% (v / v) alcohol, extracting water-containing suspension liquid of alcohol extract by petroleum ether, dichloromethane and ethyl acetate in a fractional manner to obtain an ethyl acetate active part, and extracting residual water-phase extracting liquor by n-butyl alcohol, and evaporating out the solvent to obtain a n-butyl alcohol part. The clinacanthus nutans anti-cancer active part disclosed by the invention is at least one of the clinacanthus nutans polysaccharide, the water-soluble part, the ethyl acetate part and the n-butyl alcohol part, can be used for preparing an anti-tumor drug, and has characteristics of being high in life rate of elongation, and small in toxic or side effect.

Description

technical field [0001] The invention belongs to the field of extracting anticancer active parts, and in particular relates to an extraction method and application of anticancer active parts of crocodile flowers. Background technique [0002] Alligator ( Clinacanthus nutans (Burm. f.) Lindau), commonly known as excellent (worry) Duncao, Zhuzhihuang, twisted flower, etc., belongs to the genus Alligatoria in the family Asteraceae, and is a medicinal plant widely used in traditional medicine in Southeast Asia, distributed in Indochina Peninsula , the Malay Peninsula, and Guangxi, Guangdong, Yunnan, and Hainan in mainland China (Chinese Materia Medica (7), Shanghai Science and Technology Press, 1999, 456-457). In Malaysia, Thailand and other places, crocodile mouth flower is used as a traditional medicinal material for the treatment of mental stress, diabetes, rheumatoid arthritis, snake bites, hepatitis and antiviral (Wanikiat P, et al., J. Ethnopharmacol. , 2008, 116(2): 234-...

Claims

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Application Information

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IPC IPC(8): A61K36/19C08B37/00A61P35/00
Inventor 黄丹民陈钧刘旭高静汤建
Owner JIANGSU UNIV
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