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Application of American cockroach extract skimmed cream in DDP, anti-tumor medicine for toxicity reduction and efficacy enhancement

An anti-tumor drug, the technology of Periplaneta americana, applied in the anti-tumor drug cisplatin with attenuated toxicity and enhanced efficiency, in the field of degreasing cream of Periplaneta americana extract, can solve neutropenia, easily produce nephrotoxicity, and cause side effects And other issues

Pending Publication Date: 2020-09-25
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cisplatin has certain toxicity when used in the treatment of cancer and can cause side effects. Moreover, cisplatin is a strong accumulative drug that is prone to nephrotoxicity. Gastrointestinal reactions are more common. Recoverable within days

Method used

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  • Application of American cockroach extract skimmed cream in DDP, anti-tumor medicine for toxicity reduction and efficacy enhancement
  • Application of American cockroach extract skimmed cream in DDP, anti-tumor medicine for toxicity reduction and efficacy enhancement
  • Application of American cockroach extract skimmed cream in DDP, anti-tumor medicine for toxicity reduction and efficacy enhancement

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The degreasing cream of the present invention is preferably obtained by self-preparation, and the preparation method preferably includes: extracting the Periplaneta americana with an ethanol aqueous solution with a volume percentage of 55 to 65%, reclaiming ethanol to obtain an extract; After dissolving, extract with petroleum ether, collect the lower liquid phase after extraction, and concentrate under reduced pressure to obtain the degreasing paste. In the present invention, when the volume percentage of the ethanol aqueous solution is 55%, it is preferred to mix the Periplaneta americana with the ethanol aqueous solution according to the ratio of 3000g: 10000mL, soak and extract at room temperature, extract 3 times, each time for 24h ; Combine the extracts, recover ethanol, and obtain extracts. In the present invention, when the volume percentage of the ethanol aqueous solution is 65%, it is preferred to mix and extract the Periplaneta americana with 8 times the amou...

Embodiment 1

[0042] C57BL / 6 mice were taken to establish the Lewis lung cancer tumor-bearing mouse model, and the successfully modeled mice were divided into 8 groups; another group of normal mice without modeling was set up, with 20 mice in each group, and the mice in each group were treated continuously for 15 days rear.

[0043] Treatment plan: DDP alone administration group 3: three doses of DDP of 1.5, 3, and 6 mg / kg were given by intraperitoneal injection for treatment, once a day;

[0044] Degreasing ointment administration group alone: ​​according to 4g / kg gavage administration, administration once a day; / kg)+DDP (3mg / kg), skim cream (4g / kg)+DDP (6mg / kg) three doses of intragastric administration (skim cream) + intraperitoneal injection (DDP), administered once a day;

[0045] Blank control (model group): intragastric administration of normal saline at a dose of 0.2mL / 10g;

[0046] Normal control (normal group): mice in this group were not modeled, and were given 0.2 mL / 10 g of ...

Embodiment 2

[0076] C57BL / 6 mice were taken to establish a Lewis lung cancer tumor-bearing mouse model, and each group was treated continuously for 15 days.

[0077] Therapeutic scheme: with embodiment 1.

[0078] Detect the number of mouse peripheral blood cells (white blood cells (WBC), neutrophils (GR), monocytes (MO), lymphocytes (LY), red blood cells (RBC) and platelets (PLT)); the number of bone marrow nucleated cells The femur of the mouse was stained with HE, and pathological examination was carried out; the proliferation of bone marrow cells was detected: the single cell suspension of bone marrow cells was prepared, and the bone marrow cell cycle of mice in each group was detected by flow cytometry, and the proliferation rate PI of bone marrow cells was calculated . PI=(S+G 2 M / G 0 / G 1 +S+G 2 M).

[0079] 2.1 The results of peripheral blood cell counts are shown in Table 6. Compared with the normal group, the numbers of white blood cells (WBC), neutrophils (GR), lymphocytes...

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Abstract

The invention provides application of American cockroach extract skimmed cream in DDP, an anti-tumor medicine for toxicity reduction and efficacy enhancement and relates to the technical field of agents for toxicity reduction and efficacy enhancement. The agent for toxicity reduction and efficacy enhancement comprises an American cockroach extract, wherein the American cockroach extract is skimmedcream. The effect of toxicity reduction and efficacy enhancement of the American cockroach extract skimmed cream is manifested in restraining transfer of cachexia cancer cells in lungs by combining the DDP; relieving liver damage caused by the DDP; prolonging survival time of a Lewis lung cancer mouse; recovering a lung index and a thymus index of the mouse, which are reduced by the DDP; recovering a B lymphocyte proliferation capacity reduced by the DDP; and recovering the NK cell killing activity reduced by the DDP. Marrow depression caused when the agent for toxicity reduction and efficacyenhancement is combined with the DDP in treatment of the Lewis lung cancer tumor-bearing mouse is improved, and manifestations include increase of the quantities of WBC, GR and RBC, which are reducedby the DDP, increase of reduction of marrow nucleated cells caused by the DDP and promotion of marrow cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of detoxifying and synergistic agents, and in particular relates to the application of Periplaneta americana extract degreasing ointment in the detoxifying and synergistic antitumor drug cisplatin. Background technique [0002] Cisplatin, also known as cisplatin, cisplatin, DDP, tin platinum, ethiplatin, cis-dichlorodiammine platinum, is a commonly used metal platinum complex, and the platinum atom in the molecule resists it. Tumor function is important. But only the cis form makes sense, and the trans form is invalid. Can cross-link with DNA strands, showing cytotoxicity. After dissolution, it can pass through the charged cell membrane without carrier transport in vivo. Due to the low concentration of chloride ions in the cell (4mmol / L), the chloride ion is replaced by water, and the charge is positive. It has the function of a bifunctional group similar to an alkylating agent, and can combine with the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K33/243A61P35/00A61P35/04
CPCA61K35/64A61K33/243A61P35/00A61P35/04A61K2300/00
Inventor 刘晓波黄丫丫施米丽刘光明李成功刘衡何正春何苗彭芳
Owner DALI UNIV
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