77 results about "Proliferation rate" patented technology

The invention discloses a culture method for improving proliferation and differentiation capacity of NK (Natural Killer) cells based on angelica sinensis-astragalus membranaceus exosomes, which comprises the following steps: firstly extracting exosomes in angelica sinensis and astragalus membranaceus, then adding the exosomes into an NK cell culture system according to a specific concentration, and regulating and controlling an NK cell signal channel by virtue of active ingredients carried by the exosomes so as to improve the proliferation and differentiation capacity of the NK cells. Therefore, the proliferation rate and differentiation maturity of the NK cells are remarkably improved. Experimental results show that compared with a traditional culture method, the proliferation multiple of the NK cells cultured through the method is increased by 28.99%-33.69%, the proportion of differentiated mature cells is increased by 15.91%-28.30%, and the cell killing activity is not remarkably reduced. The method can be widely applied to the field of immune cell treatment, provides a high-quality NK cell source for clinic, effectively solves the problems of slow cell proliferation rate, low differentiation maturity, easy activity reduction after large-scale culture and the like in traditional NK cell in-vitro culture, and has important clinical application value and industrialization prospect.

The invention discloses a tissue dissociation liquid, a tissue dissociation method and application thereof, and relates to the technical field of cell biology. In a DMEM / F12 culture medium, the tissue dissociation liquid only contains the following raw materials in concentration: 0.5 to 3 mg / mL of type II collagenase, 0.5 to 2 mg / mL of type IV collagenase, 0.5 to 2 mg / mL of hyaluronidase, 10 to 18 [mu] M of Y27632 and 80 to 150 [mu] g / mL of primocin. The tissue dissociation liquid provided by the invention can dissociate normal tissues or tumor tissues into single cells or cell clusters, the single cells obtained by the digestion method are high in proliferation rate and easy to successfully culture, and the tissue dissociation liquid has the technical advantages that the cell yield and survival rate are maintained at a relatively high level. Therefore, the tissue dissociation liquid provided by the invention has good application prospects in tumor tissue dissociation, cell line construction and organoid model construction.

The invention discloses an EST protease activity detection method which comprises the following steps: a, setting an EST experimental group, a negative control group and a blank control group; b, carrying out incubation reaction on the EST experimental group, the negative control group and the blank control group to obtain respective reaction mixtures; c, detecting the influence of the reaction mixture on breast cancer cell proliferation to obtain the proliferation rate of each group; and d, calculating the enzymatic activity of the EST protein. The method disclosed by the invention is simple and easy to implement, good in repeatability and capable of effectively detecting the activity of the EST protease.

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

This invention belongs to the field of biomedical research, specifically relating to the use of the human CFAP65 gene as a target in the preparation of therapeutic or diagnostic drugs for gastric cancer. Extensive and in-depth research has revealed that downregulating the expression of the human CFAP65 gene using RNAi methods can effectively inhibit the proliferation of gastric cancer cells and effectively control the growth process of gastric cancer. The siRNA or nucleic acid constructs containing the siRNA sequence, or lentiviruses provided by this invention, can specifically inhibit the proliferation rate and growth of gastric cancer cells, thereby treating gastric cancer and opening up new directions for gastric cancer treatment.

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belongs to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

Methods are provided for generating unlimited immune cells with increased lifespan and high proliferation rate by modifying them to express BCL6 and genes that promote cell survival. Further methods are provided for generating and using said unlimited immune cells to treat diseases such as cancer.

The invention relates to a method for efficiently increasing the somatic embryo occurrence rate in the later stage of picea crassifolia embryonic tissue proliferation and continuously obtaining regenerated plants. The method mainly comprises the following steps of pretreatment before differentiation of embryonic tissues subjected to long-term subculture preservation, somatic embryo differentiation and somatic embryo germination and somatic embryo seedling transplanting. In order to solve the technical problem that excellent cell lines are difficult to continuously use due to the fact that the proliferation rate and the somatic embryo occurrence rate are obviously reduced after the picea crassifolia embryogenic tissue is subcultured for a long time, the invention constructs a set of efficient somatic embryo generation and plant regeneration technology suitable for the later proliferation period of the picea crassifolia embryogenic tissue. According to the method, the proliferation rate and somatic embryo occurrence rate of the embryogenic tissue are greatly improved by pretreating the embryogenic tissue in the later proliferation stage, and the problem that the proliferation rate and somatic embryo differentiation rate of the picea crassifolia embryogenic tissue are rapidly reduced along with the prolongation of subculture time is effectively solved by combining with an optimized culture medium formula in the somatic embryo differentiation stage.

This invention relates to the field of biomedical polymer materials and surface modification engineering, specifically to a posterior scleral reinforcement material and its preparation method. It comprises a polymer matrix and a bioactive modified layer formed by the oxidative crosslinking of a heterobifunctional polymer coupling compound. This copolymer structure includes anchoring groups, hydrophilic linkers, and bioactive groups. Its core principle is to utilize the flexible extension of the hydrophilic linkers to reduce steric hindrance, fully exposing the bioactive groups to the material surface, thereby achieving efficient recognition and binding to integrin receptors. This invention effectively solves the problems of bioinertness of the synthesized polymer matrix and lack of cell recognition sites, achieving a cell proliferation rate more than twice that of the unmodified matrix, demonstrating the material's excellent bioactivity and cell compatibility.

The invention discloses a composition of type III collagen and beta-glucan and application of the composition in repairing vaginal mucosa injury, and belongs to the technical field of biological medicine, and the composition comprises the following effective components: type III collagen with the concentration of 0.3-1.8 mg / mL and beta-glucan with the mass concentration of 0.1-0.8%. The collagen III provides structural support required by tissue repair, the beta-glucan improves inflammation microenvironment, regulates immune response and jointly promotes proliferation and migration of damaged vaginal epithelial cells, the cell proliferation rate and the scratch healing rate are far higher than those of a group using any component independently, and rapid repair of mucosal lesions can be achieved.

The invention discloses a rumen fibroblast line as well as a construction method, a culture method and application thereof, the rumen fibroblast line G8 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: C2025332, the rumen fibroblast line G8 has a typical fibroblast-like form and can be stably passaged, and the rumen fibroblast line G8 can be used as a rumen fibroblast cell line. The cell culture medium also has the characteristic of highly expressing fibroblast marker genes and the function of tolerating and utilizing rumen core metabolite butyric acid, and the proliferation rate and metabolic activity of the cell culture medium under the condition that 15mmol / L butyric acid is used as a main energy source are obviously superior to those of glucose culture; according to the construction method of the rumen fibroblast line, the steps of function screening and molecular identification are introduced, so that the identity authenticity of the constructed cell line can be ensured; the culture method of the rumen fibroblast line is simple and effective, culture medium materials are easy to obtain, and culture conditions are easy to realize; the rumen fibroblast line can be used for rumen in-vitro research, and is beneficial to clarification of a mechanism for regulating rumen interstitial metabolism and tissue repair by butyric acid.

The invention discloses a medical-grade PEEK composite material for 3D printing and a preparation method of the medical-grade PEEK composite material, relates to the technical field of 3D printing, and solves the problem of low biological activity of an existing PEEK material. The medical-grade PEEK composite material is prepared from the following raw materials in parts by weight: 80-95 parts of a polyether-ether-ketone matrix, 5-15 parts of a bioactive filler, 0.5-5 parts of an interface modifier and 0.1-3 parts of a heat conduction and nucleation enhancer, the bioactive filler comprises nano-hydroxyapatite and bioactive glass in a weight ratio of (1-5): (5-1). The natural bone environment can be effectively simulated, the biological activity of the composite material is improved, and cell adhesion and proliferation are promoted, so that the cell proliferation rate is increased, and the antibacterial effect and the mechanical property of the composite material can be effectively improved.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The invention belongs to the technical field of food-borne functional factors, and particularly relates to a bioactive glycopeptide as well as a preparation method and application thereof. According to the application, casein glycomacropeptide is taken as a substrate, specific protease is adopted for carrying out directional enzymolysis reaction on the casein glycomacropeptide, and after primary separation and purification by hydrophilic interaction chromatography and secondary purification by cation exchange chromatography are carried out, the bioactive glycopeptide with the molecular weight of less than 1,500 Da and more than 80% of components and the random coil secondary structure content of more than 35% is obtained. A system performance test shows that in an in-vitro cell experiment, the bioactive glycopeptide prepared by the embodiment of the invention can remarkably improve the proliferation rate of preosteoblasts and lay a sufficient cell quantity foundation for bone cell generation; meanwhile, the activity of an osteogenic early differentiation key marker ALP is effectively up-regulated, and the differentiation process of preosteoblasts towards the osteoblast direction is accelerated; in addition, the expression quantity of osteogenesis-related regulatory protein calcium-sensitive receptors is remarkably increased, and the function improvement of cells to mature bone cells is further promoted.

This invention discloses a culture medium, preparation method, and culture method for the industrial-scale preparation of mesenchymal stem cells, belonging to the field of cell therapy technology. The culture medium comprises a basal medium, cell growth factors, cell proliferation promoters, a vitamin mixture, a nucleoside mixture, and an amino acid mixture; wherein the cell growth factors are selected from one or more of TGF-β, bFGF, VEGF, PDGF, EGF, and IGF. Through the synergistic effect of the composite nutrient system, this invention significantly improves the proliferation rate and cell viability of mesenchymal stem cells, maintains stem cell stemness, trilineage differentiation ability, and secretion levels of key cytokines (VEGF, HGF, TGF-β, IL-10, etc.); it can be stably and continuously passaged up to the 15th generation without abnormal changes in genetic material. This culture medium contains no animal-derived components, has high batch-to-batch stability, and is cost-controllable, making it suitable for GMP-level large-scale industrial preparation of mesenchymal stem cells from various sources such as umbilical cord, bone marrow, adipose tissue, and placenta.

This invention discloses a strain of Rhodotorula glutinis I7Y2 that promotes cell proliferation in a serum-free environment and its applications. Rhodotorula glutinis ( Rhodotorula mucilaginosa The yeast strain I7Y2 was deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on March 13, 2026, with accession number GDMCC No: 67951. This invention has a good effect on promoting Vero cell proliferation, specifically manifested in: (1) the yeast involved in this invention can produce various active substances such as amino acids, polypeptides, sanshool, and inositol to meet the nutritional needs of cell growth; (2) it can effectively improve the viability and density of Vero cells in the basal culture medium; (3) it can effectively improve the proliferation rate and migration rate of Vero cells in the basal culture medium; and (4) it can effectively reduce the apoptosis rate of Vero cells and the expression of apoptosis-related genes in the basal culture medium. Therefore, Rhodotorula glutinis I7Y2 has great application potential in promoting cell proliferation, especially in serum-free culture products.

The invention relates to the technical field of collagen, in particular to expression and application of recombinant humanized collagen. The recombinant collagen comprises AXBYCZ (AXBYCZ); wherein X and Z are independently selected from 0 or 1, and Y is an integer greater than or equal to 1; x and Z are not 0 at the same time; the structural domain A is a collagen N-terminal peptide or a peptide fragment thereof, the structural domain B is a Gly-Xaa-Yaa structure, and the structural domain C is a collagen C-terminal peptide or a peptide fragment thereof; the recombinant collagen not only shows excellent cell compatibility including relatively high cell viability, cell adhesion rate and cell proliferation rate, but also has the effects of resisting inflammation and inhibiting melanogenesis, and can promote synthesis of type I collagen, type III collagen and elastin, so that the recombinant collagen can be used for preparing the anti-inflammatory and anti-melanogenesis collagen. The method can be widely applied to the technical fields of biological medicine, medical equipment, cosmetics and biological engineering.

The present application belongs to the field of cell biology, specifically by adding recombinant human DFF45 protein, Z-VAD-FMK, Cyclosporin A, bFGF, N-Acetylcysteine, regulating the activity and effect of apoptosis-specific nuclease, reducing the apoptosis rate of umbilical cord mesenchymal stem cells, and also can improve the proliferation effect of umbilical cord mesenchymal stem cells, the proliferation rate of umbilical cord mesenchymal stem cells is increased by 17.53%, and the apoptosis rate of umbilical cord mesenchymal stem cells by ordinary conventional culture method is reduced from 14.32% to 9.81%, effectively solving the core problem of stem cell proliferation attenuation, apoptosis rate increase in the existing culture technology, maintaining the biological activity and quality stability of stem cells in the long-term subculture process, providing reliable technical support and experimental basis for the large-scale culture, in vitro expansion and subsequent clinical transformation application of umbilical cord mesenchymal stem cells.

This utility model belongs to the technical field of cell culture equipment, specifically relating to a mesenchymal stem cell hypoxia culture device. It includes a culture chamber, which consists of an outer door, an inner partition door, a chamber body, and a shelf. The chamber body has a hollow structure, and the shelf is installed inside the chamber. The inner partition door is connected to both sides of the front end of the chamber body and closes it. The outer door is connected to the chamber body and closes the inner partition door. The sides of the chamber body also have connecting holes that penetrate the cavity. A gas filter is installed on the pipe connected to the connecting holes. The bottom of the chamber body also has a flow guide mechanism for collecting condensate. This utility model's mesenchymal stem cell hypoxia culture device reduces the impact of opening and closing the door on the culture environment by using a partitioned cabinet door, while simultaneously disinfecting and filtering the gas entering the culture chamber, thereby improving the proliferation rate of stem cell culture.

The application provides a key preparation method of bird's nest peptide and bird's nest peptide for pregnant women, and the preparation method of the bird's nest peptide comprises the following steps: soaking dry bird's nest in pure water at 0-4 DEG C for 2 h at a material-to-liquid ratio of 1:10, beating and then drying to obtain soaked bird's nest; mixing the soaked bird's nest with pure water at a mass ratio of 1:15-20, sealing and then stewing at 121-123 DEG C for 12-15 min, repeating the stewing once after cooling to room temperature to obtain fresh stewed bird's nest; carrying out in-vitro digestion experiment on the fresh stewed bird's nest to simulate the digestion process of the fresh stewed bird's nest in the gastrointestinal tract, and obtaining digested bird's nest; centrifuging the digested bird's nest to obtain supernatant, treating the supernatant, and then carrying out protein sequencing; comparing the protein sequencing with an online database, screening out a peptide segment with high matching degree and a response value greater than 10 9 The bird's nest peptide can significantly improve the proliferation rate of nerve cells, and provides a cell basis for the development of the nervous system.

This invention belongs to the field of psoriasis diagnosis and treatment technology, specifically involving the application of circNCAPH and diagnostic / therapeutic agents for psoriasis. Abnormally high expression of circNCAPH in psoriatic lesions was detected by qRT-PCR, suggesting that circNCAPH holds promise as a diagnostic marker for psoriasis. Due to the excellent silencing effect of siRNA, after ensuring that circNCAPH was suppressed, CCK8 and apoptosis experiments were performed on HaCaT keratinocyte cell lines with silenced circNCAPH. Compared to the NC group, the proliferation rate of cells in the siRNA group was significantly slower, and the proportion of late-stage apoptotic cells was significantly increased, indicating that silencing circNCAPH inhibited keratinocyte proliferation. Therefore, inhibiting circNCAPH can treat psoriasis, which has profound clinical significance and important prospects for widespread application.

This application discloses a muscle stem cell resuscitation and protection washing solution, a quality assessment kit, and a method of use, belonging to the field of cell biology technology. In this application, vitamin E succinate and mannan peptide are used as core antioxidant components, synergistically combined with Gynostemma pentaphyllum polysaccharide, Portulaca oleracea polysaccharide, trehalose, L-proline, and magnesium sulfate heptahydrate to construct a four-in-one resuscitation and protection washing solution that provides isotonic buffering, extracellular protection, intracellular protein stabilization, antioxidant effects, and mitochondrial stabilization. This increases the survival rate of muscle stem cells after resuscitation to over 93%. The kit integrates a multi-dimensional detection system for cell resuscitation and protection washing solution, cell viability, proliferation rate, Pax7 / MyoD double-positive stemness assessment, and cryopreservation damage, achieving closed-loop management of the entire process of muscle stem cell resuscitation, protection, and quality assessment. The double-positive rate of stemness markers reaches over 90%, providing reliable quality assurance for cell bank management and clinical applications.

The invention belongs to the technical field of medical materials, and particularly relates to a preparation method of medical high-biocompatibility porous hydrogel, and the preparation method comprises preparation of a hydrogel photocuring system, coating and Faraday reaction. In the Faraday reaction process, sodium ions or potassium ions in the hydrogel sheet move towards the cathode to form a concentration difference in the hydrogel, chloride ions and hydrogen ions in the hydrogel sheet generate a large amount of gas at the same time, bubbles are generated in the hydrogel by utilizing the gas or substance concentration change generated by the Faraday reaction, and the bubbles are uniformly distributed; therefore, a uniform aperture structure is formed. According to the method, the cytotoxicity is remarkably reduced, in the Faraday reaction process, high-activity oxidation and reduction generated by the electrode can be subjected to rapid and efficient chemical reaction with unpolymerized monomers in uv polymerization, polymerization is promoted to be initiated again, and the situation that the residual monomers are adverse to wounds is avoided. The porous hydrogel prepared by the invention is verified by an MTT (Methyl Thiazolyl Tetrazolium) method cytotoxicity test method, and the relative proliferation rate of cells is greater than or equal to 90%.

The present application relates to a kind of recombinant human type III collagen and its preparation method and application, belong to the field of bioengineering technology.The amino acid sequence of the recombinant human type III collagen described in the present application is as shown in SEQ ID No.7, is through the directional arrangement of five characteristic triple helix domain and the expansion of polymerization, combined with high-density fermentation process of Pichia pastoris, successfully break through the technical barrier of 4.5g / L target protein concentration.The present application research shows that the recombinant protein at 0.5% concentration not only maintains more than 80% cell viability, more exhibits significant synergistic effect-promote fibroblast migration rate to increase more than 29% compared with blank control group, keratinocyte proliferation rate increases 40.62%, adhesion rate increases 6.53%, provides innovative solution for developing new collagen product with high-efficiency expression and multiple biological activities.

The application discloses functions and application of KIF5B gene in regulating physiological activity of myoblasts, and belongs to the technical field of animal cell culture. By investigating influences of KIF5B knockdown and overexpression on proliferation of myoblasts, it is found that KIF5B knockdown can significantly inhibit DNA synthesis speed of myoblasts, thereby inhibiting proliferation. In addition, overexpression of KIF5B can significantly inhibit expression of MYOD and MYOG, improve DNA synthesis speed of myoblasts, thereby significantly improving proliferation rate of myoblasts. The application provides an important key molecular target and technical support for efficient and rapid culture of myogenic cells.

The invention discloses a medicine for inhibiting gastric cancer cell proliferation based on fatty acid metabolism regulation, and a composition, a preparation method and application thereof, and belongs to the technical field of anti-tumor medicines and metabolism regulation medicines. The drug comprises an FA2H catalytic product which is (R)-2-hydroxy fatty acid or a pharmaceutically acceptable salt thereof; the composition comprises an FA2H catalytic product, an SCD1 inhibitor and an FASN inhibitor, and by synergistically inhibiting fatty acid uptake, synthesis and oxidative metabolism, the proliferation rate and migration ability of gastric cancer cells are remarkably reduced. The core problems that in the prior art, single target inhibition is prone to escape, chemotherapy drug resistance is high, and clinical transformation is difficult are solved.

This disclosure belongs to the field of cell culture technology, and specifically relates to a serum-free culture medium for MSCs and its preparation method. The culture medium contains a fixed ratio of basic nutrients and six key regulatory factors, namely transferrin, ascorbic acid, human serum albumin, human fibroblast growth factor rhFGF, platelet-derived growth factor PDGF-BB, and transforming growth factor TGF-β1. By optimizing the combination and concentration of these six key regulatory factors, the proliferation rate and activity of MSCs are significantly improved. It also provides four suitable formulations: high proliferation, high activity, balanced, and economical. The culture effect is stable and reproducible, with high safety and a simple process, making it suitable for laboratory research and large-scale clinical-grade MSC culture.

The application relates to a stem cell culture density optimization control method and system, and relates to the technical field of stem cell culture, and comprises the following steps: K culture boxes and P culture dishes are configured; when mesenchymal stem cells are cultured, the P culture dishes are randomly and equally distributed to the K culture boxes; P cell imaging sequences in a historical time window are monitored; P real-time cell proliferation rates and morphological characteristics are obtained through analysis; the P culture dishes are similar clustered based on the same; K similar culture dish sets and K cell proliferation rate averages are determined; the K similar culture dish sets are mapped and placed in the K culture boxes; and the K culture boxes are respectively optimized and adjusted in culture environment parameters based on the K cell proliferation rate averages, so that K adaptive environment parameters are obtained, and the K culture boxes in a future time window are controlled. The application solves the problems that in traditional stem cell culture, a unified culture environment is adopted, targeted culture is lacked, and the culture density control precision is low and the cell quality is unstable.

The invention discloses a fibronectin-based bioactive fragment and application thereof. The amino acid sequence of the bioactive fragment is shown as SEQ ID NO: 1, 2 or 3. The invention also relates to a nucleotide sequence (as shown in SEQ ID NO: 4, 5 or 6) for coding the fragment, a recombinant expression vector containing the nucleotide and a host cell. According to the application of the obtained bioactive fragment in culture of primary mesenchymal stromal cells (MSCs), the fragment can significantly improve the adherence efficiency and proliferation rate of the MSCs, effectively solves the technical problems of difficult in-vitro culture, slow amplification speed and easy differentiation of the MSCs, provides a new tool for large-scale production of high-quality MSCs, and has wide application prospects. Wide application prospects are realized in the fields of tissue engineering and regenerative medicine.