87results about How to "Short amplification time" patented technology

The invention discloses a H3 subtype flu quick-detecting type classifying method based on an RT-LAMP technique. The method comprises the following steps: firstly, analyzing the HA gene sequence of the H3 subtype flu virus in a flu virus gene group sequence database, designing highly special and conserved RT-LAMP special primers and six primers corresponding to eight areas of target genes, wherein four primers are FIP, BIP, F3 and B3, and the other two primers are loop primers LP1 and LP2; secondly, treating a sample to be detected in advance, and extracting the total RNA of the sample to be used as a reaction template; thirdly, configuring an RT-LAMP reaction system; fourthly, mixing the RNA template into the RT-LAMP reaction system, arranging the RT-LAMP reaction system in a water bath, and carrying out RT-LAM enlargement at constant temperature; and fifthly, identifying the enlarged reaction product. The result shows that the reaction product contains H3 subtype flu virus when the reaction product is positive; and the reaction product is H3 subtype flu virus when the reaction product is negative.

The invention provides a method and system for individual recognition and paternity identification of an unknown sample. The method includes: extracting the DNA of the unknown sample; acquiring the typing result of 17 loci contained by the DNA, i.e. 14 autosome STR loci, 2 Y-chromosome loci, and the sex determination locus Amelogenin, with the STR loci being D6S1043, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51 and FGA, the Y-STR locus being DYS448, and the Y-chromosome insertion / deletion site being M134; and carrying out individual recognition and paternity identification according to the genotypes of the 17 loci of the unknown sample. The invention also provides the system for individual recognition and paternity identification of the unknown sample. The scheme involved in the invention can realize simultaneous detection of autosome STR loci and Y-chromosome loci, and also can shorten the detection time, thus improving the efficiency of individual recognition.

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL / mL, human Source AB type serum 5‑15 μL / mL, human transferrin 5‑15 μg / mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.

In the case that a refresh operation is carried out which is independent from an external access operation, both a data access method of a semiconductor memory device, and a semiconductor memory device are provided by which time suitable of each of these external access operation and refresh operation is set. While a time-measuring start signal “SIN” is entered into a path switching means, the path switching means is connected to either a first timer section or a second timer section under control of an external-access-operation-start-request signal REQ(O) and a refresh-operation-start-request signal REQ(I). Both the first and second timer sections measure both time “τO” and time “τI” to output a time-measuring stop signal “SOUT.” The measuring time “τO” corresponds to differential amplification time of a bit line pair when the external access operation is carried out, whereas the measuring time “τI” corresponds to differential amplification time when the refresh operation is carried out. Alternatively, the measuring time “τO” may be varied by reading / writing operations so as to be set. As a consequence, proper amplification time can be set every operation mode.

The invention discloses a human autosomal STR polymorphic site compound amplification kit and application thereof. The kit contains specific amplimers for amplifying the following 25 genetic locus: D3S1358, D13S317, D7S820, D16S539, D16S539, D1S1656, PentaE, TPOX, TH01, D2S1338, CSF1PO, Penta D, D19S433, vWA, D21S11, D18S51, D6S1043, Amelogenin, D8S1179, D8S1179, D5S818, D12S391, FGA, D22S1045, Yindel, D2S441 and D10S1248. The kit disclosed by the invention has the advantages that on the premise that the cumulative identification ability of the genetic loci is enhanced, the sensitivity is improved, all the genetic 25 loci can be detected under the condition that the DNA template amount is below 50pg and 29 cycle amplification is carried out, so that a complete genotype can be obtained. According to the human autosomal STR polymorphic site compound amplification kit disclosed by the invention, the quantity of the genetic loci can better satisfy continuous expansion of database construction and detection requirements of the genetic loci are added; the kit is efficient and stable, so that the detection rate of a detection material in a field case is improved and the expansion time isshortened.

The invention relates to a method for breeding lean-type Chinese Huai pigs in a multi-gene pyramiding manner based on growth traits thereof. The method comprises the following operating steps: 1, carrying out DNA (deoxyribonucleic acid) extraction on the genome of the pig; 2, designing primers, more particularly, designing the PCR (polymerase chain reaction) amplification primers according to the gene sequences of insulin-like growth factor-I (IGF-I), pituitary specific transcription factor-I (PIT-I), liver X receptor alpha (LXR alpha) and melanocortin-4 receptor (MC4R); 3, carrying out the PCR; 4, carrying out restriction fragment length polymorphism (RFLP); and 5, carrying out the correlation analysis on the polymorphism and growth traits of genes. According to the analysis, the method can determine that the single growth rate of the pyramided gene with the gene type thereof being AADDGGFF is the highest one; the method can prevent the genes of other offsprings from being isolated after the selective reservation for breeding, thus achieving the optimal effect of the growth traits of the offsprings on the four gene types; and the method can stabilize the inheritance, particularly lead the genes of the growth traits to become homozygous within one generation, thus accelerating the cultivation of the lean-type Chinese Huai pigs.

The invention discloses an inhibition method for induced differentiation of hair follicle stem cells into vascular endothelial cells, comprising: (1), isolating rat hair follicle stem cell; (2), culturing the rat hair follicle stem cells; (3), purifying the rat hair follicle stem cells; (4), inhibiting the induced differentiation of the rat hair follicle stem cells into vascular endothelial cells. In the inhibition method provided herein, vascular endothelial cell growth factor 165 is used for the first time as an inducing factor so that the rat hair follicle stem cells efficiently and directionally differentiate into vascular endothelial cells; the formation and growth of vascular endothelial cells generated by induced differentiation of the hair follicle stem cells can be adjusted, an effective healing of a wound is promoted; it is also possible to provide seed cell sources for solving the problems in vascularization of tissue engineering skin, cell-transplant therapy of ischemic disease and the like.

The invention relates to a primer composition, kit and method for detecting the novel coronavirus. The primer composition includes a first primer pair and a second primer pair which are used for detecting the N gene of the novel coronavirus, and also includes a third primer pair and a fourth primer pair which are used for detecting the ORFlab gene of the novel coronavirus. The method comprises the steps: using the primer composition, adopting the genomic DNA of a to-be-tested sample as a template, performing a constant-temperature amplification reaction under the action of thymine DNA glycosylase, MLV reverse transcriptase and Bst DNA polymerase, and performing quantitative analysis on the test results under the action of fluorescent dye so as to detect the novel coronavirus with high specificity and high sensitivity. The four primers are used, bases of the primers are specially modified, and the thymine DNA glycosylase and Bst DNA polymerase are added to the reaction, so that exponential amplification of target sequences of the N gene and the ORFlab gene are realized under the condition of constant temperature, and the sensitivity and specificity of detection are improved greatly.

The invention discloses a swine acute diarrhea syndrome coronavirus primer combination and a kit and method thereof. The swine acute diarrhea syndrome coronavirus primer combination and the kit and method thereof aim at providing an SADS (swine acute diarrhea syndrome) detecting primer and method high in sensitivity and specificity. The swine acute diarrhea syndrome coronavirus primer combinationis composed of a forward inner primer of SADS-FIP, a backward inner primer of SADS-BIP, a forward outer primer of SADS-F3, a backward outer primer of SADS-B3, a forward loop primer of SADS-LF and a backward loop primer of SADS-LB. The detecting method of the swine acute diarrhea syndrome coronavirus primer combination comprises 1) extracting RNA (ribonucleic acid) of virus from a sample; 2) takingthe extracted RNA as a template, and establishing an RT-LAMP(reverse transcription-loop-mediated isothermal amplification) system through the RT-LAMP primer combination of SADS to perform RT-LAMP ata constant temperature of 60-65 DEG C; 3) detecting amplification products through a DEAOU-308C thermostatic fluorescence detector. The swine acute diarrhea syndrome coronavirus primer combination andthe kit and method thereof belong to the field of biological detecting technology.

The invention discloses a culture medium capable of promoting the division growth of mesenchymal stem cells, which comprises a serum-free basic culture medium and an additive added on the basis of theserum-free basic culture medium, wherein the additive comprises a hibiscus mutabilis extract, seaweed polysaccharide, astragaloside IV, human serum albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and vitamin A; the hibiscus mutabilis extract has antioxidant and cell activating activity, and can effectively promote the growth of cell metabolic by compounding seaweed polysaccharide and astragaloside IV; human serum albumin, transferrin, glutamine and vitamin A provide essential nutrient substances for the growth of stem cells; platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and other cytokines together promote the rapid growth and proliferation ofstem cells; the culture medium not only can improve the growth activity of mesenchymal stem cells, shorten the culture time, promote the expression of cell growth factors, but also can maintain the stem cell activity of the differentiation potential of mesenchymal stem cells; stem cells are not differentiated in daily culture, thereby providing convenience for scientific research.

The invention provides a PCR fluorescent detector. In the detector, a PCR reaction tube is arranged in a first through hole, a second through hole and a third through hole, so the bottom of the PCR reaction tube is positioned at the third through hole, the level of PCR reaction liquid in the PCR reaction tube is positioned at the first through hole, the bottom of the PCR reaction tube is heated by a 95 DEG C heating plate, and the level of the PCR reaction liquid in the PCR reaction tube is heated by a 60 DEG C heating plate, thus producing temperature difference between the upper and lower ends of the PCR reaction tube. The temperature difference causes the PCR reaction liquid in the PCR reaction tube to flow from the bottom high-temperature region to the top low-temperature region to form countercurrent. Due to the temperature difference and countercurrent, DNA can be continuously amplified in the PCR fluorescent detector, continuous heating and cooling for reaching the temperature required by different stages of DNA amplification are not needed, therefore, the PCR fluorescent detector provided by the invention can save the amplification time and further shorten the detection time of the PCR detector.

The invention belongs to the technical field of edible fungi cultivation and specifically relates to a cultivation method for oyster mushrooms. The cultivation method comprises the following steps: S1, strain activation; S2, strain amplification; S3,inoculation; S4, hairy fungus cultivation; S5, mushroom producing management; and S6 harvesting. A culture medium is prepared from reasonable raw materials, and a nutrient solution is sprayed after former-stubble mushrooms are harvested, so that the yield of the oyster mushrooms is remarkably increased.

The invention relates to the technical field of biological gene detection, and aims to provide an ovarian premature senility related gene whole exon amplification and detection method. The method comprises the following steps: extracting genome DNA from a detection material; using totally 120 primers shown in SEQ ID NO:1-120, carrying out an amplification test on all exons of 9 genes, namely FMR1,FOXL2, FSHR, POF1B, INHA, NOBOX, GDF9, BMP15 and FIGLA, wherein totally 61 PCR reactions in the amplification test and the negative control are carried out synchronously, and the Tm value range of the 120 primers is 57-62 DEG C; detecting an amplification product by adopting agarose electrophoresis and a gel imaging system; and analyzing the sequencing result of the amplification product by usingan automatic sequencer. All exons of 9 genes related to premature ovarian failure are synchronously amplified through optimized PCR amplification specificity, and a detection raw material is providedfor downstream detection. The method is simple and convenient to operate, short in amplification time, high in specificity and good in repeatability. The detected positive rate is greatly improved, and the application prospect is wide.

The invention relates to a gene chip, a primer set and a kit for single-tube detection of non-deletion alpha-thalassemia and beta-thalassemia, and belongs to a molecular diagnosis technology. According to the present invention, based on the direct multiplex PCR and reverse dot blot combined detection principle, the corresponding amplification primers and the corresponding probes are designed according to the mutation or deletion sites of each genotype, the primer is labeled with biotin, the probe is labeled with amino, a gene chip is used as substrate, the probe is immobilized on the DNA chip,the PCR product amplified by the specific primer is hybridized with the probe, and the diagnosis of thalassemia is performed by interpreting a signal coloring box.

The invention discloses a recombinant enzyme amplification detection method for HPV16 and HPV18 genotypes, a test strip and application thereof. The sequences of a primer pair for the HPV16 genotype are shown in SEQ ID NO.1 and SEQ ID NO.2 respectively. The sequences of a primer pair for the HPV18 genotype are shown as SEQ ID NO.3 and SEQ ID NO.4 respectively. The double lateral flow chromatography test strip comprises a supporting plate, wherein the upper end face of the supporting plate comprises a sample loading zone, a labeling zone, a marking-off zone and a water absorption zone which arearranged in sequence; the labeling zone is bound with latex microspheres coupled with streptavidin, and detection lines T1 and T2 and a quality control line C which are bound with an FITC antibody, adigoxin and a second antibody respectively. The detection method is good in specificity, high in sensitivity, good in specificity, good in repeatability, easy to operate and high in applicability.

The invention provides an InDel molecular marker for identifying a radish flower color control gene and application thereof. A forward primer sequence of the InDel molecular marker is as shown in SEQ ID NO.4, reverse primers comprises a first reverse primer and a second reverse primer, a sequence of the first reverse primer is as shown in SEQ ID NO.5, and a sequence of the second reverse primer is as shown in SEQ ID NO.6. Compared with the prior art, the InDel molecular marker has the beneficial effects that the radish flower color can be detected in the seedling stage by detecting the deletion or insertion of the provided specific fragment, and the InDel molecular marker can be used for subsequent identification and breeding.

The invention belongs to the field of biomedicines, relates to a colorectal cancer detection kit, and more particularly relates to a colorectal cancer detection kit taking excrement as a detection sample. The detection kit is characterized in that an Alu detection reagent and PvPP are contained. Through multi-time exploration in numerous colorectal cancer markers, Alu is discovered that the sample can be directly diluted for PCR amplification detection without going through complex steps of cell disruption, DNA extraction and the like on the premise of proper simple pretreatment, and good sensitivity and specificity are obtained.

The invention relates to a detection method for sensitively and rapidly detecting staphylococcus aureus enterotoxin B, comprising the following steps: heating H1 and H2 with concentration of 5Mum at 95 DEG C for denaturation for 5min, heating an SEB aptamer at 85-95 DEG C for denaturation for 5-10min, then taking 6.7-33.3muL of SEB aptamer with concentration of 1muM, adding 100muL of SEB, mixing and then adding 80-100muL of hybrid buffer solution, and reacting for 30min at 37 DEG C; adding 6.7-33.3muL of 1muM H0, and reacting for 30min at 37 DEG C; adding 10-30muL of mixed reaction system of 100muL of H1 with concentration of 5muM and 100muL of H2 with concentration of 5muM; reacting for 45min at 37 DEG C constant temperature, and measuring the fluorescence value; compared with a conventional detection technology based on nucleic acid aptamer, the detection sensitivity is reduced by 2 orders of magnitudes.

The present invention relates to a PCR amplification mechanism for a dPCR integrated microfluidic chip. The PCR amplification mechanism comprises a preheating flow channel and a circulating flow channel communicating in sequence, wherein the preheating flow channel is used to heat droplets to a first preset temperature; the circulating flow channel includes a plurality of high temperature sectionsand a plurality of low temperature sections arranged at intervals, so that the droplets are subjected to high temperature, low temperature... high temperature and low temperature circulating flow inthe circulating flow channel; the beginning end of the preheating flow channel communicates with the tail end of a droplet output pipeline; and the tail end of the circulating flow channel communicates with a droplet detection mechanism. The tiny droplets are firstly preheated to the first preset and then subjected to high temperature, low temperature...high temperature and low temperature circulating flow in the circulating flow channel, and rapid amplification of the droplets is realized. The amplification time is shortened, and the detection efficiency is greatly improved on the premise ofensuring accuracy.

The invention discloses primer pairs and probes for detecting thalassemia genes. The primer pairs and probes include primer pairs and probes of beta-thalassemia CD41 / 42 and alpha-thalassemia-SEA; theprimer pair of the beta-thalassemia CD41 / 42 is a sequence 1 and a sequence 2, and the probes are a sequence 3 and a sequence 4; and the primer pair of thealpha-thalassemia-SEA is a sequence 5 and a sequence 6 or a sequence 5 and a sequence 7, and the probes are a sequence 8 and a sequence 9. The invention further provides a use method of the primer pairs and probes for detecting the thalassemia genes. The use method comprises the following steps of S1, collecting a sample and extracting DNA; S2, carrying out an amplification reaction by utilizing one kind of primer pair and probes; S3, detecting the fluorescence intensity and determining the genotype; and S4, repeating the process by utilizing the other kind of primer pair and probes. The method has the beneficial effects that the fluorescence detection accuracy is enhanced through the designed primer pairs and probes and the two kinds of primer pairs of the same type; and rapid, accurate and harmless detection is realized by extracting free DNA of plasma and blastocyst culture media of pregnant women.

The invention relates to an isothermal amplification method for detecting cry1Ac-transfected sugarcane. The method comprises the steps of extracting sugarcane template DNA (Deoxyribonucleic Acid), establishing an isothermal amplification system and identifying an isothermal amplification product. According to the isothermal amplification method for detecting the cry1Ac-transfected sugarcane, four specific primers are designed aiming at base sequences of an exogenous target gene cry1Ac of stem-borer-resistant transgenic sugarcane, and a chain-displacement amplification reaction is carried out under the action of polymerase Bst, so that the specificity is high; meanwhile, the amplification reaction can be completed by only a water bath kettle and a normal-temperature low-speed centrifuge capable of completing instant centrifugation, so that instruments and equipment required are simple, the amplification cost is relatively lower compared with that of the conventional PCR (Polymerase Chain Reaction) technology, which needs a gel scanning system and a PCR (or real-time PCR) instrument, which are expensive, and the like, the amplification time is short, the amplification efficiency is high, and the result viewing is visual and convenient due to visual color reaction; and after the system is established, extracted DNA of a sugarcane genome directly serves as a template, so that the method is applicable to the cry1Ac-transfected sugarcane screening of laboratories and fields and the detection and tracking of cry1Ac ingredients and has the advantages of low cost, rapidness, sensitivity, simplicity and accuracy.

The invention discloses a method for digitally and quantitatively detecting nucleic acid based on a DNA (deoxyribonucleic acid) chip. The method comprises the following steps: fixing an amplification primer to the chip; amplifying target nucleic acid through a single nucleic acid molecule template; marking information on a probe and amplified nucleic acid hybridization detection marked gene or reading out molecule information of amplified nucleic acid through a minisequencing process to read out signals of all cloned points, wherein each signal point represents the number of original target DNA molecules; performing statistics on the number of signal points and calculating the number of nucleic acid. A new method is provided for quantitative analysis of nucleic acid molecules, and a quick, accurate and cheap nucleic acid detection technology is built.

The invention discloses a method for increasing the compatibility of a multi-PCR (Polymerase Chain Reaction) primer and belongs to the field of biological detection. The method comprises the followingsteps: designing a pair of specific primers Fs and Rs and common primers Fg and Rg aiming at each target gene; carrying out enrichment and amplification on specific primers Fg+Fs and Rg+Rs with common primer labels; then carrying out index amplification by adopting the common primer labels Fg and Rg; and finally, detecting by adopting a probe hybridization or non-probe hybridization manner according to a detection platform. According to the method provided by the invention, each reaction component concentration, such as a primer concentration and an Mg<2+> concentration, in each step of PCR amplification is controlled to control reaction conditions of the PCR amplification, such as annealing temperature and annealing time, so that specific and sensitive amplification of multi-target genesis realized. The method has strong specificity and can ensure that the amplification efficiency of different pathogen nucleic acids is similar, and the deviation of the traditional multi-PCR amplification is avoided; and denaturalization does not need to be carried out to realize melting in a detection process.

The invention provides a breast cancer 21 gene detection kit. A constant-temperature fluorescent quantitative PCR amplification enzyme mixed solution is added into a breast cancer 21 gene detection kit, the newest constant-temperature fluorescent quantitative PCR amplification technology is introduced, the problem that traditional fluorescent quantitative PCR consumes long time is solved, the whole amplification time is shortened to 40 min to 1 h from 2 h to 3 h, operation is simpler and more convenient than that of a traditional method, and the time cost of medical inspectors is greatly saved. Meanwhile, the time for doctors and patients to wait for results is shortened, and an optimal scheme can be provided for subsequent chemotherapy of the patients earlier and faster.

The invention discloses a CDA primer group and a kit for detecting African swine fever virus (ASFV), and an application of the CDA primer group and the kit. The CDA primer group is a primer group for amplifying a conserved region fragment of the African swine fever virus, namely ASFV-MF-3 / ASFV-MR-3, wherein the nucleotide sequence of the ASFV-MF-3 is as shown in SEQ ID NO. 5, and the nucleotide sequence of the ASFV-MR-3 is as shown in SEQ ID NO. 6. The primer group provided by the invention is high in sensitivity and strong in specificity, and the kit prepared from the primer group can quickly and accurately detect whether a sample to be detected contains the African swine fever virus or not. In addition, the visual kit provided by the invention can provide great convenience for on-site detection in places such as farms, customs, ports and community vegetable markets with low professional degrees.

The invention provides a primer composition for detecting clostridium difficile. The primer composition is designed based on a conserved sequence of a toxin gene tcdB of the clostridium difficile. Theinvention further provides a reagent kit for detecting the clostridium difficile, and the reagent kit comprises the primer composition, enzymes for identifying and excising an unconventional DNA basein a strand in double-strand DNA, and DNA polymerase having the function of strand displacement. The invention further provides a method for detecting the clostridium difficile. According to the method provided by the invention, target sequences can be correctly detected, the specificity is good, and the detection sensitivity and the detection efficiency are greatly improved.

The invention relates to a preparation method of artificial skin by taking VEGF165 gene modified hair follicle stem cells as seed cells. The preparation method comprises following step: 1) hair follicle stem cells, which possess excellent cellular morphology and are in growing period, are collected; 2) the collected hair follicle stem cells are purified; 3) the hair follicle stem cells are identified; 4) lentivirus are packaged; 5) the hair follicle stem cells are infected by lentivirus; 6) gelatin sponge three-dimensional tissue scaffolds are prepared; 7) the gelatin sponge three-dimensional tissue scaffolds which are preserved at a temperature of 4 DEG C are taken, and the modified hair follicle stem cells are inoculated in the gelatin sponge three-dimensional tissue scaffolds or on the surfaces of the gelatin sponge three-dimensional tissue scaffolds by injection so as to obtain the artificial skin. According to the preparation method, the VEGF165 gene modified hair follicle stem cells are taken as the seed cells, rapid and large-scaled amplification of the hair follicle stem cells can be realized by in vitro culture, resources of the hair follicle stem cells are quite abundant, and the hair follicle stem cells are capable of reducing immunological rejection. In addition, the hair follicle stem cells are transfected and modified by VEGF165 gene, so that novel engineering blood vessel systems with expansion and contraction functions can be formed, and problems of easily caused artificial skin necrosis in transplanting and low survival rate are solved perfectly.

The invention discloses a serum-free medium for expanding human mesenchymal stem cells, which is based on the medium for culturing stem cells, adding linoleic acid, human AB type serum, and platelet-derived growth factors. The invention also discloses a culture method for human mesenchymal stem cells. The serum-free medium of the present invention uses multiple cytokines in combination reasonably, which not only improves the purification effect of mesenchymal stem cells, but also shows that the morphology of human mesenchymal stem cells is still uniform even after 30 passages, thus ensuring The safety of human mesenchymal stem cells improves the expansion speed of cells, reduces the expansion time and reduces the cost.

The invention relates to a mycoplasma pneumoniae (MP) rapid detection primer group and a kit. The mycoplasma pneumoniae rapid detection primer group comprises an upper stream primer, a lower stream primer and a probe which detect a mycoplasma pneumoniae gene group P1 gene sequence. The kit comprises a lysate solution, a reconstitution fluid, an EMA reaction tube containing primer probes, a positive quality control product and a negative quality control product. The primer group can be specifically combined with the mycoplasma pneumoniae P1 gene, and cooperate with EMA (Enzymes Mediated Amplification, a multienzyme mediated nucleic acid amplification technology, namely a normal temperature nucleic acid amplification technology) to prepare the mycoplasma pneumoniae detection kit, and the mycoplasma pneumoniae rapid detection primer group and kit have the advantages of easy to operate, short in consumed time, high in sensitivity and specificity and the like.