14 results about "Gelatin sponge" patented technology

The application discloses a method for inducing NK cells based on a porous material. The method for inducing NK cells provided by the application is based on a device containing a mesh culture tank and porous gelatin sponge and a culture medium, and can efficiently induce iPSCs-NK. The method solves the problems that current differentiation culture technologies of iPSC-NK rely on feeder layer cells of animal origin, the differentiation efficiency is low, and the purity is insufficient and needs to be further sorted. The method has the potential for large-scale industrial production.

The utility model relates to a non-contact type multifunctional neurosurgery brain cotton and gelatin sponge transfer tray which comprises an upper tray and a lower tray which can be combined, a hollow cylinder is arranged at one corner of the upper tray, a pin hole column matched with the hollow cylinder through a pin shaft is arranged at one corner of the lower tray, a plurality of partition plates are arranged in the upper tray, and the partition plates are arranged in the lower tray. The upper tray is divided into a plurality of brain cotton storage areas through the partition plates, and a downward-concave circular groove used for storing an aneurysm temporary clip is formed in the lower tray. The utility model designs a double-layer opening and closing type handheld tray which can be opened in a fan-shaped manner when in use. Four kinds of brain cotton of different sizes and models can be placed on the first layer, and meanwhile brain cotton threads can be stretched and cannot be wound mutually; gelatin sponges with different sizes and models can be placed on the second layer, and an aneurysm temporary clip can be placed on the circular sunken part. When a doctor uses the device, a nurse can deliver the brain cotton and the gelatin sponge at the same time, delivery time is saved, and the device is of great significance in dealing with cerebral hemorrhage in an emergency.

The present application relates to the technical field of biology, and particularly relates to an anti-oxidation gelatin sponge and a preparation method thereof. The preparation method comprises the following steps: preparing a lipid nanoparticle containing three nucleic acids of Oct4, Sox2 and Klf4; grafting phenylboronic acid to a gelatin skeleton to obtain a phenylboronic acid gelatin; dissolving the phenylboronic acid gelatin in a lipid nanoparticle solution to form a mixed solution, then adding a tannic acid solution and a hyaluronic acid solution into the mixed solution in sequence to obtain the anti-oxidation gelatin sponge. The anti-oxidation gelatin sponge can be directly implanted or injected into a high fracture site (such as a vertebral body or a femoral neck) of an osteoporosis patient, and can achieve the purposes of treating osteoporosis and preventing first or secondary fracture by long-term regulation of local bone metabolism, reduction of bone resorption and increase of bone density.

The invention discloses a tissue engineering vascular smooth muscle remodeling method based on a conducting polymer and application thereof. The invention finds that the conductive polymer plays a role in promoting the migration of vascular smooth muscle cells and guiding the functional remodeling of the vascular smooth muscle cells. According to the method, a conducting polymer synthesized by biological enzyme is stably modified on a tissue engineering blood vessel in three modes of direct soaking, click chemical grafting or gelatin sponge stent loading. The conductive modified intravascular stent can reconstruct an electrophysiological microenvironment similar to a natural blood vessel, can significantly guide smooth muscle cells to orderly migrate and arrange along the circumferential direction of the blood vessel, and promotes the smooth muscle cells to be transformed to contraction phenotypes expressing markers such as alpha-SMA, SM22alpha, CNN1 and the like, so that a smooth muscle layer close to an autologous blood vessel is structurally and functionally reconstructed. According to the invention, the bottleneck of insufficient functional remodeling of the tissue engineering vascular smooth muscle layer in the prior art is broken through, and a new strategy is provided for regeneration treatment of cardiovascular diseases.

The utility model discloses a gelatin sponge cutting device, solved two clamps only can be to the both sides of gelatin sponge and carry out the location, and the location effect is not good problem, it includes the base, the top of base is provided with sponge limiting component, and sponge limiting component includes the support plate of fixed mounting in the top of base, the top of support plate is provided with sponge cutting assembly, and the top of support plate isohedral angle is provided with four side portion limiting units, and the top of support plate isohedral angle is provided with four sliding slots, and four sliding slots form cross shape, and side portion limiting unit includes the limiting plate, and the outer wall of one end of limiting plate and one side limiting plate is close, and the bottom of limiting plate is provided with push adjustment unit, the utility model discloses, in the work, through the square limiting area of formation between the four limiting plates of setting, can to square limiting area carry out length and width adjustment through the movement of relative two limiting plates, and the convenient limiting of different size gelatin sponge.

The utility model relates to gelatin sponge production technical field, and disclose a kind of feeding device for gelatin sponge production, solve the problem of current raw material mixing time is longer, it includes stirring bucket, the top of stirring bucket is equipped with adjusting mechanism and inclination mechanism, the upper of stirring bucket is equipped with raw material barrel, adjusting mechanism includes two slide bars fixedly in the top of stirring bucket, the outside of two slide bars respectively movably sleeved with rear sliding plate and front sliding plate, rear sliding plate and front sliding plate between fixedly connected with guide column, the outside of guide column movably sleeved with guide plate, raw material barrel is arranged in the top of guide plate, rear sliding plate side close to front sliding plate is rotatably connected with upper screw rod, the end of upper screw rod away from rear sliding plate is fixedly connected with side shaft;The utility model, it is convenient for guide plate to slide along guide column to make raw material barrel move back and forth, to make raw material be conveniently poured evenly in the inside of stirring bucket, shorten mixing time.

The application discloses a composite hemostatic sponge and a preparation method and application thereof. The sponge takes gelatin sponge as a base body, and is coated with a polymer coating formed by copolymerization of acrylic acid-N-succinimidyl ester and a hydrophilic modified monomer on the surface; the hydrophilic modified monomer is selected from one or more of acrylic acid, 4-acryloylmorpholine or dimethylaminoethyl acrylate. The preparation method comprises the following steps: dissolving monomers in a solvent to initiate a copolymerization reaction, precipitating, cleaning and drying the reaction solution to obtain a copolymer, uniformly coating the copolymer on the surface of the gelatin sponge after redissolving the copolymer, and drying. The composite hemostatic sponge can break away from the dependence on the blood coagulation cascade reaction, can still maintain high adhesion strength in a wet and anticoagulation environment, and does not sacrifice the porous blood absorption characteristics of the sponge itself.

The utility model belongs to the technical field of stirring mixing kettle, disclose a kind of stirring mixing kettle for gelatin sponge production, including kettle body, the inner wall of kettle body is fixedly arranged with heater, stirring mechanism is arranged in kettle body, still fixedly arranged with connecting barrel and hot air blower on the kettle body, and the inner top of kettle body is communicated with one end of hot air blower, and the other end of hot air blower is communicated with connecting barrel;Stirring mechanism is provided with shaft and outer stirring rod, and outer stirring rod is fixedly arranged on the shaft, wherein inner passage is formed in the shaft, and inner through hole is formed in outer stirring rod, and inner through hole is communicated with connecting barrel by inner passage;The utility model, by the cooperation of the hot air blower, shaft and outer stirring rod, the hot air flow generated by hot air blower can play the role of auxiliary heating for the dissolution of gelatin, and the hot air flow can also play the role of impact on gelatin in water, to avoid the laminar flow phenomenon in the stirring process.

Provided is a method for constructing a chronic thromboembolic pulmonary hypertension animal model, comprising: taking 180-200 gram adult healthy male SD rats, allowing the rats to freely take water and food; injecting a soluble gelatin sponge solution into the jugular vein of the rats; after 1 day, subcutaneously injecting a Sugen5416 solution; then allowing the rats to freely take water and food for 28 days, to obtain a chronic thromboembolic pulmonary hypertension rat model.

The invention discloses a treatment method for intractable pneumothorax under the guidance of CT (Computed Tomography), which comprises the following steps of: 1, carrying out clinical examination before an operation on an intractable pneumothorax patient needing to be treated through clinical consultation, carrying out consultation judgment according to the clinical examination, and signing an informed consent before the operation; the position and the form of the broncho pleural fistulae are accurately displayed through CT three-dimensional reconstruction, the position of the fistulae is accurately punctured through a PTCB needle under local anesthesia, the purpose of plugging the fistulae is achieved by selecting proper plugging materials such as a medical adhesive / iodized oil mixture, a spring ring and gelatin sponge, and the clinical applicability of the intractable pneumothorax plugging operation under CT guidance is high; required CT and plugging materials are conventionally available in primary hospitals; the device is simple in structure, convenient to operate, accurate in plugging and capable of being carried out only under local anesthesia, most patients can tolerate multiple operations, minimally invasive, safe, efficient and instant in effect, has very important diagnosis and treatment significance on treatment of most refractory pneumothorax, and is worthy of clinical application and popularization.

The utility model relates to the technical field of cutting devices, and discloses a gelatin sponge cutting device which solves the problems that an existing gelatin sponge cutting device is low in cutting speed, cannot rapidly and continuously cut a large number of gelatin sponges and reduces the cutting efficiency. A mounting frame is fixedly mounted in the middle of the top of the bottom plate, supporting plates are fixedly mounted on the two sides of the top of the bottom plate, a connecting frame is fixedly mounted on one side of the mounting frame, a driving motor is fixedly mounted on one side of the connecting frame, and conveying belts are fixedly mounted on the tops of the two supporting plates. Two supporting legs are fixedly mounted at one ends of the bottoms of the two conveying belts, two cutting knives are symmetrically arranged on the upper portion and the lower portion in the mounting frame, a transmission assembly is arranged at the output end of the driving motor, and the transmission assembly is in transmission connection with the two cutting knives; the gelatin sponge cutting device is high in cutting speed and capable of rapidly and continuously cutting a large number of gelatin sponges, and the cutting efficiency is improved.

This invention relates to the field of neurosurgical technology and discloses a method for preparing a gelatin sponge-brain pad composite material for neurosurgical procedures. The method includes preparing a gelatin sponge subunit: mixing medical-grade gelatin, glutaraldehyde, and deionized water, and preparing a cross-linked medical gelatin sponge using a freeze-drying method, which is then categorized into three sizes: S, M, and L. The method also includes preparing a brain pad subunit: sewing medical-grade absorbent cotton with barium contrast agent, and then hydrophilically treating the brain pad. This method for preparing a gelatin sponge-brain pad composite material for neurosurgical procedures standardizes and integrates the gelatin sponge and brain pad according to the surgical type. Preoperatively, there is no need to separately count the two instruments; postoperatively, the quantity is checked according to the packaging unit. This significantly simplifies the instrument management process, reduces the workload of medical staff, and decreases the error rate in instrument counting. Simultaneously, it can accurately match the component specifications and quantities according to different surgical types—basic, delicate, and emergency—avoiding material waste or insufficient supply.

The invention relates to the technical field of biological materials, in particular to a carboxymethyl cellulose gelatin composite multifunctional hemostatic sponge and a preparation method thereof.The carboxymethyl cellulose gelatin composite multifunctional hemostatic sponge comprises a carboxymethyl cellulose / gelatin sponge base material and a combined antibacterial agent, and the antibacterial agent is polylysine; the preparation method comprises the following steps: chemically crosslinking carboxymethyl cellulose and gelatin to prepare a carboxymethyl cellulose / gelatin sponge base material, immersing the carboxymethyl cellulose / gelatin sponge base material into an antibacterial agent solution, and freeze-drying to obtain the antibacterial sponge. The antibacterial agent is combined, so that the antibacterial and bacteriostatic effects are remarkably improved, the broad-spectrum antibacterial effect is achieved, and the infection risk of a wound is reduced; through cross-linking of carboxymethyl cellulose and gelatin, the mechanical stability of the composite sponge is improved, and the composite sponge has good elasticity, shape memory performance and biocompatibility and has a good effect on filling and hemostasis of irregular and narrow deep wounds.

The invention relates to fluid gelatin particles with combined sizes as well as a preparation method and application of the fluid gelatin particles. The specific preparation method of the fluid gelatin particles with the combined size comprises the following steps: carrying out physical or chemical crosslinking to obtain gelatin particles or gelatin sponge, then carrying out mechanical grinding, then sieving by using screens with different pore diameters, collecting gelatin particles with different sizes, and finally combining the particles with different sizes according to a proper and determinable volume ratio to obtain the fluid gelatin particles with the combined size. The combined size fluid gelatin particles provided by the invention are pre-swelled to obtain the hemostatic fluid gelatin. And the pre-swelled gelatin particles have good flowability and extremely low swelling property. The fluid gelatin particles with the combined size have good biocompatibility, biological activity and lacuna plugging effect, and can be used for development of fluid hemostatic materials.