51 results about "Gelatin sponge" patented technology

The embodiment of the utility model provides a puncture suite, which relates to the field of puncture equipment and comprises a sleeve needle, a solid needle core, a hollow biopsy needle, a pusher and an iodine-containing gelatin sponge rod. The sleeve needle is provided with a positioning channel, and the solid needle core and the hollow biopsy needle can be selectively inserted into the positioning channel; the iodine-containing gelatin sponge rod is used for being inserted into the positioning channel after the solid needle core and the hollow biopsy needle are separated from the sleeve needle; and the pusher is matched with the positioning channel in a sliding manner and is used for pushing the iodine-containing gelatin sponge rod into the puncture channel from the positioning channel. The puncture suite is good in hemostatic effect, high in puncture success rate and high in puncture safety.

The invention relates to the technical field of medical instruments, and discloses a tympanic stent and a using method thereof.The tympanic stent comprises a top ring, a first connecting rod, a length adjusting rod, a bottom ring, a second connecting rod, a height adjusting rod and a supporting rod, a first notch is formed in the front side of the top ring, and the two ends of the first notch are connected with first free silk threads respectively; the two ends of the first notch are connected with the length adjusting rod, a second notch is formed in the front side of the bottom ring, the two ends of the second notch are connected with second free silk threads respectively, and the height adjusting rod is arranged on the rear side between the top ring and the bottom ring. The whole tympanic cavity stent is in a drum shape, the stent is arranged in a tympanic cavity, and the tympanic cavity does not need to be filled with absorbable materials such as gelatin sponge; the size of the top ring and the size of the bottom ring can be adjusted through the length adjusting rod to be matched with the tympanic chamber, and the inclination angle of the top ring can be adjusted to be matched with the residual tympanic membrane through the height adjusting rod and knotting of the first free silk thread and the second free silk thread. The invention further provides a using method of the support.

The invention discloses a multi-section gelatin sponge filling type puncture channel plugging device and a plugging method thereof.The multi-section gelatin sponge filling type puncture channel plugging device comprises a sleeve which is of a long and thin tubular structure, a cylindrical base is integrally formed at the rear end of the sleeve, a wedge-shaped inlet is formed in the base, and scale marks are arranged on the outer surface of the sleeve; the inner core is formed by sequentially arranging a plurality of sections of independent short columnar medical gelatin sponge units, and the inner core is accommodated in the front section of the cannula; the diameter of the pushing rod is matched with the inner diameter of the sleeve, the pushing rod is inserted into the sleeve, and an expanded handheld part is arranged at the rear end of the pushing rod; according to the scheme, the puncture channel can be safely, conveniently and efficiently blocked after the percutaneous intervention puncture channel is established, a small amount of bleeding of the puncture channel is effectively treated, and the risks of secondary bleeding and massive bleeding are reduced or eradicated, so that the related operation safety is improved, and the occurrence rate of related adverse events is reduced.

The application discloses a method for inducing NK cells based on a porous material. The method for inducing NK cells provided by the application is based on a device containing a mesh culture tank and porous gelatin sponge and a culture medium, and can efficiently induce iPSCs-NK. The method solves the problems that current differentiation culture technologies of iPSC-NK rely on feeder layer cells of animal origin, the differentiation efficiency is low, and the purity is insufficient and needs to be further sorted. The method has the potential for large-scale industrial production.

The invention discloses a high-bionic in-vivo immune microenvironment in-vitro skin model and application, and belongs to the technical field of biological medicine. The preparation method of the in-vitro skin model of the high-bionic in-vivo immune microenvironment comprises the following steps: firstly, adding a culture medium into the lower layer of a six-pore plate, then adding a mouse bone marrow cell suspension, then putting the six-pore plate into a Transwell chamber, flatly laying separated mouse skin on an absorptive gelatin sponge, putting the absorptive gelatin sponge into the Transwell chamber, adding the culture medium into the Transwell chamber, and then putting the mouse skin into the transwell chamber to obtain the in-vitro skin model of the high-bionic in-vivo immune microenvironment. Placing the six-hole plate in a cell incubator for culturing to obtain the in-vitro skin model. According to the in-vitro skin-bone marrow cell co-culture system, an immune environment is provided for the in-vitro skin by introducing bone marrow cells, so that the culture environment of the in-vitro skin is more complete, the difficulties that cell components are simple and the immune environment is deficient at present can be solved, and the in-vitro skin-bone marrow cell co-culture system is more in line with the 3R principle and is high in bionic property and low in cost.

The invention provides a coaxial puncture needle beneficial to implantation of gelatin sponge, which comprises a needle tube assembly and a needle rod assembly, and the needle rod assembly is nested in the needle tube assembly; the needle tube assembly comprises a needle tube body and a needle base, the needle base and the needle tube body are arranged in a sleeved mode, and the needle rod assembly penetrates into the needle base and the needle tube body. A horn mouth is formed in the end, away from the needle tube body, of the needle base and used for the needle rod assembly to plug the gelatin sponge into the needle tube body. A visible window is formed in the connecting portion of the needle tube body and the needle base. The needle has the advantages that the flat-head needle rod is additionally arranged on the basis of an original pointed-head needle rod, gelatin sponge is conveniently stuffed into the coaxial needle through the flat-head needle rod, the aim of stopping bleeding rapidly, conveniently and effectively is achieved, and the most common complication bleeding in needle biopsy is greatly reduced.

The invention belongs to the technical field of medicine, and discloses a medicinal sponge sheet for inhibiting growth of nasal polyp, the medicinal sponge sheet is composed of a medical grade absorptive gelatin sponge base material, triamcinolone acetonide and hydroxypropyl methyl cellulose, the medicinal sponge sheet adopts the medical gelatin base material with the porosity of 95% and the water absorption expansion rate of less than or equal to 30%, the size is 60mm * 20mm * 5mm, each sheet loads 60mg of triamcinolone acetonide, and the hydroxypropyl methyl cellulose is added into the medicinal sponge sheet. And 10% of hydroxypropyl methyl cellulose with the molecular weight of 35000 Da and the viscosity of 700 mPa.s is supplemented to construct a slow release system. Efficient and safe treatment of chronic nasosinusitis with nasal polyp is achieved through multi-dimensional innovative design, the three-dimensional interpenetrating pore structure of the medical gelatin base material provides an ideal carrier for drug release, 60%-70% of triamcinolone acetonide is continuously released within 7 days through the gradient slow release technology, a local high-concentration microenvironment is constructed, and the effect of treating chronic nasosinusitis with nasal polyp is achieved. The device directly acts on the polyp root to block an inflammation cascade reaction; in the formula, hydroxypropyl methyl cellulose is used as a core slow-release carrier.

The invention discloses a synthetic method of gelatin sponge particles. The synthetic method comprises the following steps: preparing a gelatin solution; adding a water-soluble developing agent, and uniformly stirring to obtain gelatin gel; drying and crushing to obtain pretreated gelatin powder; putting the pretreated gelatin powder and water into a high-pressure reaction kettle together, and uniformly stirring to obtain gelatin dispersion liquid; adding a cross-linking agent and a physical foaming agent into the gelatin dispersion liquid to increase the pressure in the reaction kettle to a first pressure; stopping stirring and releasing the pressure in the high-pressure reaction kettle to prepare the gelatin sponge particles. According to the method provided by the invention, firstly, gelatin gel with a developing effect is prepared, and then the gelatin gel is dried and crushed to obtain pretreated gelatin powder with uniform particles, so that the gelatin powder, a cross-linking agent and a physical foaming agent are mixed in water and then cross-linked to form developable gelatin sponge particles with relatively uniform particle sizes; the problems that in the background technology, foam structures are prone to being damaged, and the particle morphology is uncontrollable when gelatin sponge particles are manufactured are solved.

The utility model relates to a non-contact type multifunctional neurosurgery brain cotton and gelatin sponge transfer tray which comprises an upper tray and a lower tray which can be combined, a hollow cylinder is arranged at one corner of the upper tray, a pin hole column matched with the hollow cylinder through a pin shaft is arranged at one corner of the lower tray, a plurality of partition plates are arranged in the upper tray, and the partition plates are arranged in the lower tray. The upper tray is divided into a plurality of brain cotton storage areas through the partition plates, and a downward-concave circular groove used for storing an aneurysm temporary clip is formed in the lower tray. The utility model designs a double-layer opening and closing type handheld tray which can be opened in a fan-shaped manner when in use. Four kinds of brain cotton of different sizes and models can be placed on the first layer, and meanwhile brain cotton threads can be stretched and cannot be wound mutually; gelatin sponges with different sizes and models can be placed on the second layer, and an aneurysm temporary clip can be placed on the circular sunken part. When a doctor uses the device, a nurse can deliver the brain cotton and the gelatin sponge at the same time, delivery time is saved, and the device is of great significance in dealing with cerebral hemorrhage in an emergency.

The present application relates to the technical field of biology, and particularly relates to an anti-oxidation gelatin sponge and a preparation method thereof. The preparation method comprises the following steps: preparing a lipid nanoparticle containing three nucleic acids of Oct4, Sox2 and Klf4; grafting phenylboronic acid to a gelatin skeleton to obtain a phenylboronic acid gelatin; dissolving the phenylboronic acid gelatin in a lipid nanoparticle solution to form a mixed solution, then adding a tannic acid solution and a hyaluronic acid solution into the mixed solution in sequence to obtain the anti-oxidation gelatin sponge. The anti-oxidation gelatin sponge can be directly implanted or injected into a high fracture site (such as a vertebral body or a femoral neck) of an osteoporosis patient, and can achieve the purposes of treating osteoporosis and preventing first or secondary fracture by long-term regulation of local bone metabolism, reduction of bone resorption and increase of bone density.

The invention relates to the technical field of materials, in particular to a gelatin sponge microsphere product and a preparation method thereof. The preparation method comprises the following steps: dissolving gelatin into deionized water to obtain a dispersed phase solution; vegetable oil and a surfactant are mixed to prepare a continuous phase solution; vegetable oil and a surfactant are mixed, and a receiving phase solution is obtained; respectively injecting the dispersed phase solution and the continuous phase solution into a microfluidic device to obtain gelatin liquid drops; introducing the gelatin liquid drops into the receiving phase solution, cooling and solidifying to obtain a crude product; adding the crude product into a first cross-linking solution, carrying out primary cross-linking immobilization, removing an upper-layer solution, and continuously adding a second cross-linking solution for further cross-linking immobilization to obtain a semi-finished microsphere product; and removing the upper-layer solution from the microsphere semi-finished product, and cleaning to obtain the microsphere. According to the invention, the alcohol-containing cross-linking liquid is adopted to carry out microsphere cross-linking, so that the cross-linking agent is in rapid contact with the microspheres to carry out cross-linking; the preparation of small-size liquid drops is facilitated, and the morphology of the liquid drops can be maintained, so that the surfaces of the cross-linked microspheres are smooth and have no wrinkles.

The application relates to the technical field of biological medicines, and particularly discloses a gelatin embolization particle and a preparation method and application thereof. The preparation method of the gelatin embolization particle comprises the following steps: taking gelatin as raw material, and performing the following steps of dissolving, foaming, freeze-drying, heat crosslinking, crushing and screening, and sterilization; the foaming step is as follows: nitrogen is introduced into a gelatin aqueous solution with a concentration of 20-100 g / L under a liquid surface, foaming is performed at a rotating speed of 2500-3500 rpm, until no obvious liquid is present at the bottom of a container, and the foaming volume is 1-10 times the original liquid volume; the heat crosslinking step comprises the following steps: the gelatin sponge body is vacuum dried at 60-90 DEG C for 20-30 min, and then vacuum dried at 110-130 DEG C for 10-36 h, and the gelatin embolization particle is obtained. The gelatin embolization particle obtained by the preparation method has high porosity, appropriate water absorption, good in-vitro degradation, excellent suspension performance and catheter passing performance.

The invention belongs to the technical field of medicines, and particularly relates to tetrahedral framework nucleic acid hydrogel as well as a preparation method and application thereof. The invention provides tetrahedral framework nucleic acid hydrogel which is formed by self-assembly of tetrahedral framework nucleic acid, chemical modification is not needed, and the preparation method is simple, mild and efficient; compared with Y-type DNA hydrogel, the hydrogel has the advantages that the mechanical property is more excellent, and the stability is higher; antibacterial drugs or burn drugs do not need to be added, and the hemostatic performance is more excellent than that of gelatin sponge; the antioxidant performance is equivalent to that of a powerful antioxidant; compared with commercially available burn cream, the burn cream has better performance of promoting healing of burn wounds; the biodegradability and the biological safety are excellent. The hydrogel disclosed by the invention has a good application prospect in the aspect of treating chronic wounds, such as burn wounds.

The invention relates to the technical field of three types of implanted embolism medical instruments, in particular to gelatin sponge embolism particles as well as a preparation process and application thereof. The preparation process of the gelatin sponge embolism particles mainly comprises the following steps: preparing a gelatin solution and a formaldehyde solution; stirring and foaming the gelatin solution to obtain gelatin foam; adding a formaldehyde solution, and increasing the rotating speed to continue foaming; freezing and solidifying, unfreezing and crushing to obtain coarse sponge particles 1; extracting, centrifuging, cleaning and dehydrating the coarse sponge particles to obtain finely-cleaned sponge particles; and carrying out freeze drying, and sieving and grading the sponge particles to obtain the gelatin sponge embolism particles. The gelatin sponge embolism particles prepared by the invention are uniform in structure, low in residue, high in biological safety, strong in clinical operability and obvious in embolism effect.

The invention relates to a three-dimensional culture method for hair papilla cells and application of supernate of the hair papilla cells in hair regeneration. The invention relates to a three-dimensional culture method for hair papilla cells. The method comprises the following steps: (1) preparing hair follicle hair papilla cells into a cell suspension; (2) cutting the gelatin sponge, and dropwise adding polylysine; (3) wetting the gelatin sponge with the culture medium; (4) sucking out the culture medium, adding a cell suspension, and incubating, so that the cells are fully colonized; and (5) adding a complete culture medium after colonization, immersing all the sponge, culturing for a period of time, then replacing with a culture medium containing L-ascorbic acid, culturing until cells are aggregated into balls, then replacing with a serum-free culture medium, culturing for 24 hours, and then collecting supernate. According to the method, the in-vivo inductivity of the hair papilla cells subjected to 3D culture by using the gelatin sponge scaffold is maintained, the survival ability and differentiation potential of the cells are improved, and the secretion ability of growth factors for promoting hair regeneration is remarkably improved, so that the effect of promoting hair regeneration is achieved.

The utility model provides a gelatin sponge compression cutter which comprises a base, a rocker, a pressing block, a male die, a female die and a push rod, the base is provided with a supporting frame, the rocker is in threaded connection with the supporting frame and penetrates through one end of the supporting frame to make contact with the pressing block, the female die is fixedly installed on the base, the male die is movably installed on the base, and the push rod is installed on the base. The female die and the male die are oppositely arranged, the push rod penetrates through a side supporting frame installed on the base to make contact with the male die, and the push rod is used for pushing the male die to be close to or away from the female die along the surface of the base. And one specification or multiple specifications can be cut at a time. The gelatin sponge can be compressed and cut accurately, efficiently, safely and conveniently, the operation requirements of doctors are met, the operation effect is improved, operation time is shortened, and the satisfaction degree of the doctors is improved.

The invention relates to the field of animal models, in particular to a method for constructing a chronic tympanic membrane perforation rat model. The construction method comprises the following steps: under a microscope, perforating a rear quadrant of a tympanic membrane of a unilateral ear of a rat, embedding a gelatin sponge into the perforated part for a period of time, and then removing the gelatin sponge to obtain a chronic tympanic membrane perforated rat model; wherein the gelatin sponge is a gelatin sponge infiltrated with a drug solution, and the drug solution is a dexamethasone solution. According to the construction method of the chronic tympanic membrane perforation rat model, the success rate of modeling can be increased on the basis of reducing the death rate of animals, animal waste caused by drug toxicity and the risk of cancerogenic substance exposure of experimenters are reduced, and the construction method has important significance in tympanic membrane perforation research.

The utility model discloses a percutaneous bile duct puncture channel plugging device, and relates to the technical field of medical instruments. The device comprises a puncture tube, a sheath tube is arranged in the puncture tube, an air bag is arranged in the sheath tube, and a protective layer is arranged on the surface of the air bag. The blocking assembly is arranged on the surface of the puncture tube, and the blocking assembly comprises a supporting tube. By arranging the air bag, the air bag can be moved into the puncture channel through the puncture tube after puncture treatment is finished, the air bag is communicated with external inflation equipment through the air inlet tube, and the air bag can be inflated and expanded to support a protective layer compressed gelatin sponge for plugging and enable the gelatin sponge to be attached to the tube wall, so that the rapid plugging effect is achieved; through the arrangement of the blocking assembly, blocking and shielding can be conducted on the external wound position of a patient through the first blocking pad and the second blocking pad in the puncture treatment process, bacteria are prevented from entering, and the protection effect is achieved.

A hemostatic material with near-infrared light-controlled shape memory properties, its preparation method, and its application. Due to the shape memory effect, the sponge's shape can be restored to 90% within 25 seconds. By switching the near-infrared light source on and off, the sponge's expansion can be controlled and adjusted. The sponge's hemostatic ability has been verified in vitro and in vivo. As expected, compared with commercial medical gelatin sponges with insufficient expansion capacity and commercial medical PVA sponges with excessive expansion capacity, the PDA-TPI-PU shape memory sponge can quickly and effectively control bleeding under near-infrared light irradiation, showing potential application prospects as a hemostatic material. With excellent mechanical properties, blood compatibility, cytocompatibility, and photothermal conversion performance, the PDA-TPI-PU sponge provides a new approach to using light-controlled shape memory materials as coagulation materials for treating incompressible bleeding.

The invention provides a coaxial puncture needle supporting advanced introduction of hemostatic gelatin sponge. The coaxial puncture needle comprises a needle tube assembly, a needle rod assembly and an introduction device, the needle tube assembly comprises an outer needle tube, an outer needle seat and a Luer interface; the outer needle tube is connected with the outer needle seat, the outer needle seat is connected with the Luer interface, and the Luer interface is connected with the leading-in device; a sponge channel is formed in the guide-in device, the sponge channel is communicated with the outer needle tube, and hemostatic gelatin sponge is stuffed into the sponge channel; the needle rod assembly comprises a flat-end inner needle, a pointed-end inner needle and an inner needle seat; and the flat-head inner needle penetrates into the outer needle tube through the sponge channel. The guide-in device has the beneficial effects that the guide-in device is adopted, the gelatin sponge is stuffed into the guide-in device in advance, and the guide-in device is immediately connected to the needle tube after the puncture needle is pulled out, so that the guide-in action of the hemostatic gelatin sponge is faster, the guide-in position is more accurate, and the guide-in process is smoother.

The present invention discloses a method for synthesizing gelatin sponge particles, comprising the following steps: preparing a gelatin solution; adding a water-soluble developer and stirring uniformly to obtain a gelatin gel; drying and crushing the solution to obtain a pretreated gelatin powder; placing the pretreated gelatin powder and water in an autoclave and stirring uniformly to obtain a gelatin dispersion; adding a crosslinking agent and a physical foaming agent to the gelatin dispersion to raise the pressure in the autoclave to a first pressure; stopping stirring and releasing the pressure in the autoclave to obtain gelatin sponge particles. The method provided by the present invention first prepares a gelatin gel with a developing function, then dries and crushes the gelatin gel to obtain a pretreated gelatin powder with uniform particles, and then mixes the gelatin powder, the crosslinking agent, and the physical foaming agent in water and crosslinks to form developable gelatin sponge particles with relatively uniform particle size. This method solves the problems of the prior art in which the production of gelatin sponge particles easily causes the destruction of the pore structure and the uncontrollable particle morphology.

The embodiment of the utility model relates to a gelatin sponge slicer, and belongs to the technical field of medical instruments. The gelatin sponge slicer comprises a left leaf and a right leaf, the left leaf and the right leaf are connected in a folded mode so that the left leaf and the right leaf can be oppositely opened, the left leaf and the right leaf are each formed by stacking a plurality of separation blades at intervals, at least one side edge of each separation blade is a scattered edge, and gelatin sponge grooves are formed in the opposite faces of the left leaf and the right leaf. The gelatin sponge groove is used for placing gelatin sponge; the device further comprises a cutting tool. According to the gelatin sponge slicer provided by the embodiment of the invention, the effect of efficiently and safely preparing gelatin sponge sheets is achieved.

The invention discloses a tissue engineering vascular smooth muscle remodeling method based on a conducting polymer and application thereof. The invention finds that the conductive polymer plays a role in promoting the migration of vascular smooth muscle cells and guiding the functional remodeling of the vascular smooth muscle cells. According to the method, a conducting polymer synthesized by biological enzyme is stably modified on a tissue engineering blood vessel in three modes of direct soaking, click chemical grafting or gelatin sponge stent loading. The conductive modified intravascular stent can reconstruct an electrophysiological microenvironment similar to a natural blood vessel, can significantly guide smooth muscle cells to orderly migrate and arrange along the circumferential direction of the blood vessel, and promotes the smooth muscle cells to be transformed to contraction phenotypes expressing markers such as alpha-SMA, SM22alpha, CNN1 and the like, so that a smooth muscle layer close to an autologous blood vessel is structurally and functionally reconstructed. According to the invention, the bottleneck of insufficient functional remodeling of the tissue engineering vascular smooth muscle layer in the prior art is broken through, and a new strategy is provided for regeneration treatment of cardiovascular diseases.

The utility model discloses a gelatin sponge cutting device, solved two clamps only can be to the both sides of gelatin sponge and carry out the location, and the location effect is not good problem, it includes the base, the top of base is provided with sponge limiting component, and sponge limiting component includes the support plate of fixed mounting in the top of base, the top of support plate is provided with sponge cutting assembly, and the top of support plate isohedral angle is provided with four side portion limiting units, and the top of support plate isohedral angle is provided with four sliding slots, and four sliding slots form cross shape, and side portion limiting unit includes the limiting plate, and the outer wall of one end of limiting plate and one side limiting plate is close, and the bottom of limiting plate is provided with push adjustment unit, the utility model discloses, in the work, through the square limiting area of formation between the four limiting plates of setting, can to square limiting area carry out length and width adjustment through the movement of relative two limiting plates, and the convenient limiting of different size gelatin sponge.

Provided is a thrombin-carrying hemostatic sheet that is suitable for hemostasis during surgery, in particular, for hemostasis during spine surgery, that is convenient without preparation before use, and that is bioabsorbable and can be embedded in the body as it is. The hemostatic sheet is composed of a gelatin sponge carrying an effective amount of thrombin, wherein (A) the density is 30 to 55 mg / cm3, and (B) the shape maintaining angle in wet conditions is 55 to 120°.

The hemostatic gauze for hemodialysis comprises a gauze body, and a plurality of restraint straps are arranged on the gauze body. A contact layer, a hemostasis layer, an isolation layer and a fixing layer are sequentially arranged on the gauze body from bottom to top; the contact layer is made of a hydrophilic biocompatible material; thrombin, chitosan microspheres and gelatin sponge are arranged in the hemostatic layer; the isolating layer is made of a polytetrafluoroethylene film, and the fixing layer is made of elastic medical non-woven fabric. According to the multi-layer structural design that the gauze body is sequentially provided with the contact layer, the hemostasis layer, the isolation layer and the fixing layer from bottom to top, and all the layers are clear in function and cooperate with one another; the contact layer is in direct contact with the wound and is responsible for primary blood coagulation promotion and adhesion prevention; the hemostatic layer can stop bleeding efficiently; the isolating layer plays a dual role in blocking outside bacteria and preventing adhesion; the fixing layer ensures that the whole gauze is stably fixed at the wound part; due to the layered structural design, the hemostatic gauze can effectively meet various requirements for hemostasis and follow-up nursing of a hemodialysis puncture part.

The utility model relates to gelatin sponge production technical field, and disclose a kind of feeding device for gelatin sponge production, solve the problem of current raw material mixing time is longer, it includes stirring bucket, the top of stirring bucket is equipped with adjusting mechanism and inclination mechanism, the upper of stirring bucket is equipped with raw material barrel, adjusting mechanism includes two slide bars fixedly in the top of stirring bucket, the outside of two slide bars respectively movably sleeved with rear sliding plate and front sliding plate, rear sliding plate and front sliding plate between fixedly connected with guide column, the outside of guide column movably sleeved with guide plate, raw material barrel is arranged in the top of guide plate, rear sliding plate side close to front sliding plate is rotatably connected with upper screw rod, the end of upper screw rod away from rear sliding plate is fixedly connected with side shaft;The utility model, it is convenient for guide plate to slide along guide column to make raw material barrel move back and forth, to make raw material be conveniently poured evenly in the inside of stirring bucket, shorten mixing time.

The invention relates to a preparation method and application of a neural tissue engineering blood vessel. The preparation method comprises the following steps: preparing neural stem cell / neural precursor cell neural spheres; preparing a gelatin sponge into a hollow cylinder, adding neural spheres, and incubating to prepare a neural gelatin sponge; and penetrating the decellularized artificial blood vessel or other tissue engineering blood vessels into the neurogelatin sponge for incubation and culture to obtain the neurotized tissue engineering blood vessel. The vascular graft with a nerve secretion function is constructed by optimizing preparation and dynamic culture parameters of neural spheres and combining a cooperative processing technology of a gelatin sponge stent and an acellular vascular matrix, and is used for repairing coronary artery, carotid artery and peripheral vascular defects; the method has the advantages that rapid re-endothelialization, innervation and anti-inflammatory microenvironment formation of transplanted blood vessels can be promoted, repair and fusion of the blood vessels can be accelerated, a new neurotization scheme is provided for tissue engineering blood vessels, and a new method is provided for solving the difficult problems of reconstruction and long-term stability of transplanted blood vessels clinically.

The application discloses a composite hemostatic sponge and a preparation method and application thereof. The sponge takes gelatin sponge as a base body, and is coated with a polymer coating formed by copolymerization of acrylic acid-N-succinimidyl ester and a hydrophilic modified monomer on the surface; the hydrophilic modified monomer is selected from one or more of acrylic acid, 4-acryloylmorpholine or dimethylaminoethyl acrylate. The preparation method comprises the following steps: dissolving monomers in a solvent to initiate a copolymerization reaction, precipitating, cleaning and drying the reaction solution to obtain a copolymer, uniformly coating the copolymer on the surface of the gelatin sponge after redissolving the copolymer, and drying. The composite hemostatic sponge can break away from the dependence on the blood coagulation cascade reaction, can still maintain high adhesion strength in a wet and anticoagulation environment, and does not sacrifice the porous blood absorption characteristics of the sponge itself.

The utility model belongs to the technical field of stirring mixing kettle, disclose a kind of stirring mixing kettle for gelatin sponge production, including kettle body, the inner wall of kettle body is fixedly arranged with heater, stirring mechanism is arranged in kettle body, still fixedly arranged with connecting barrel and hot air blower on the kettle body, and the inner top of kettle body is communicated with one end of hot air blower, and the other end of hot air blower is communicated with connecting barrel;Stirring mechanism is provided with shaft and outer stirring rod, and outer stirring rod is fixedly arranged on the shaft, wherein inner passage is formed in the shaft, and inner through hole is formed in outer stirring rod, and inner through hole is communicated with connecting barrel by inner passage;The utility model, by the cooperation of the hot air blower, shaft and outer stirring rod, the hot air flow generated by hot air blower can play the role of auxiliary heating for the dissolution of gelatin, and the hot air flow can also play the role of impact on gelatin in water, to avoid the laminar flow phenomenon in the stirring process.