64 results about "Thrombin" patented technology

Disclosed are a fibrinogen solution and a thrombin solution. The fibrinogen solution comprises fibrinogen at a concentration of at least 40 mg / ml, factor XIII, pharmaceutically acceptable additives and water. The dynamic viscosity of the fibrinogen solution measured at 20° C. increases at most by 35% after storing the solution at 20° C. for 30 days. The thrombin solution comprises thrombin, pharmaceutically acceptable additives and water.

The present invention is related to the field of tissue regeneration. It concerns more particularly new processes, tubes and devices for thrombin, platelet concentrate and wound healant preparations, alone or in combination with cell extracts, cell compositions and uses thereof.

The application relates to the technical field of medical dressing preparation, and discloses a hemostatic, healing-promoting and antibacterial foam dressing and a preparation method thereof. The method uses sodium alginate and carboxymethyl chitosan as a composite polysaccharide matrix, mixes the two, adds a calcium chloride solution to crosslink a porous gel support, and then carries out freeze-drying to obtain a porous framework; gelatin and polyvinyl alcohol can be further added to enhance the mechanical strength, zeolite particles loaded with silver ions are added to achieve antibacterial performance, epidermal growth factor release microspheres are added to promote healing, and the dressing can be further subjected to heparinization, plasma surface modification, thrombin addition, salt precipitation pore formation and sterilization treatment. The dressing prepared by the method has the functions of synergistic hemostasis, antibiosis and healing promotion, has good biocompatibility and suitable mechanical strength, and has a simple preparation process, and can be used for various wound care.

The invention provides special gel for medical surgical site ice compress and a preparation method thereof, and relates to the technical field of medical treatment. The preparation raw materials at least comprise: a main polymer: polyvinyl alcohol (PVA), bacterial nanocellulose (BNC), and poly N-isopropylacrylamide; the functional additives comprise double modified starch (OSA + phosphate), nano-silver (the particle size is 20-50 nm), a thrombin-fibrinogen compound and EGF / bFGF microspheres (PLGA); and the auxiliary components comprise glycerol, citric acid and purified water. According to the multifunctional ice compress gel, a structure which is loose outside and tight inside is formed through freeze drying, the cold compress uniformity and the air circulation performance are improved, the double modified starch and the cross-linking agent cooperate, the degradation period is prolonged to 72 h, the replacement frequency is reduced, the multifunctional ice compress gel has the functions of physical cooling, hemostasis repair, antisepsis and anti-inflammation, the cooperative requirement of postoperative cold compress and wound surface nursing is met, and the multifunctional ice compress gel is worthy of popularization and application. The device is suitable for postoperative incision cold compress, burn / acute stage cooling and hemostasis of wounds and auxiliary repair of chronic wounds.

The present application relates to a method for preparing and expanding a TSCM cell in vitro. Specifically, the method comprises: 1) obtaining an isolated CD8+ T cell; 2) activating the CD8+ T cell; 3) mixing the activated CD8+ T cell, a fibrinogen solution and a thrombin solution, and culturing same under conditions suitable for cell growth, so as to obtain a TSCM cell culture; and 4) bringing the culture into contact with a dispase so as to obtain a TSCM cell. The present application also relates to a kit for preparing and expanding a TSCM cell in vitro, the use of fibrinogen and thrombin in the preparation of a kit for preparing and expanding a TSCM cell in vitro, and a TSCM cell obtained by means of the method or kit of the present application.

The invention belongs to the technical field of biomedical material engineering, and discloses a hydrogel scaffold used for 3D tumor sphere culture and drug screening, the scaffold is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition crosslinking and biopolymerization synergistically, the elastic modulus of 10-1000 Pa can be adjusted by regulating and controlling the proportion of all the components, and the hydrogel scaffold can be used for 3D tumor sphere culture and drug screening. Gel forming is completed within 5 min at the room temperature. The scaffold has the biological activity of fibrous protein and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-activity tumor balls, accurately simulate the in-vivo tumor microenvironment and remarkably improve the accuracy and repeatability of drug screening, is easy and convenient to operate, is compatible with a standard pore plate high-throughput platform and is suitable for drug sensitivity detection of personalized treatment of tumors.

The utility model provides a thrombin determination kit fixing frame convenient to expand, which relates to the technical field of medical instruments and comprises a fixing base. A fixing frame is inserted into the top end face of the fixing base, a partition plate is slidably connected into the fixing frame, a control rod is rotatably connected to the middle of the top end face of the partition plate, walking gears are symmetrically and rotatably connected to the left and right sides of the upper and lower end faces of the partition plate, and a locking pin is slidably connected into the partition plate in the middle of the top of the control rod. According to the thrombin determination kit fixing frame convenient to expand, the fixing frames are rapidly spliced through the connecting combination blocks, the connecting combination grooves and the butterfly nuts at the top and the connecting lug blocks and the connecting lug grooves at the bottom, a user can conveniently and rapidly expand the scale of the fixing frame according to actual requirements, and it is ensured that the overall structure is stable; therefore, the problems that a fixing frame is fixed in structure, single in mounting position, difficult to adapt to quantity change, low in space utilization rate and difficult to flexibly adjust a partition plate are solved.

The application belongs to the technical field of biotechnology, and particularly relates to a kind of snake venom polypeptide and application thereof.The snake venom polypeptide is polypeptide DACU-1, polypeptide DACU-2 or polypeptide DACU-3.The application provides a kind of new snake venom polypeptide, including three polypeptides, and the three polypeptides all have anti-platelet aggregation activity, and have the activity of inhibiting platelet aggregation under the stimulation of ADP, arachidonic acid, collagen, thrombin and other various activators, and have the potential to be developed into anti-platelet drugs, functional foods and health products.

The invention discloses a 5 / 8 / 5 chitosan clostridium diterpenoid compound, a biosynthetic gene cluster and application of the biosynthetic gene cluster, and relates to the technical field of biological medicines. A 5 / 8 / 5 chitin type diterpenoid biosynthetic gene cluster Thm in trichoderma harzianum is identified through a genome mining system, chitin diterpenoid compounds with diversified structures are obtained by means of heterologous expression, the obtained compounds are subjected to anti-thrombotic activity comprehensive evaluation, and the result shows that the compounds 6 and 31 can obviously inhibit vascular thrombosis, the compound 6 has a remarkable inhibition effect on collagen-induced platelet aggregation, and the compound 31 has a better inhibition capability on thrombin-induced platelet activation. The compounds 6 and 31 show powerful antithrombotic effects in artery, vein and pulmonary artery thrombosis models, so that powerful technical support is provided for development of antithrombotic drugs.

The present invention relates to a polypeptide derived from natural tridegin by structural modification, which may serve as an inhibitor of plasma coagulation factor XIIIa, and to pharmaceutical compositions comprising such polypeptide. The present invention further refers to such polypeptide or pharmaceutical composition for use in a method for treating or preventing a thrombotic event in a patient. Moreover, the invention relates to an endoprosthesis or an enzyme-linked immunosorbent assay (ELISA) plate coated with a polypeptide of the present invention. Furthermore, the invention also encompasses a recombinant fusion protein comprising this polypeptide linked to a fibrinolytic enzyme such as streptokinase, nattokinase or tPAs via a flexible peptide linker containing a thrombin-cleavable site. This construct allows selective inhibition of FXIIIa within a thrombus, while enabling thrombin-triggered release of the fibrinolytic enzyme to induce localized fibrinolysis, thereby achieving targeted dual-action thrombolytic therapy.

The application belongs to the field of thrombin apparatus and equipment, and relates to a thrombin apparatus, which comprises a frame structure, a reaction cup assembly, a reagent assembly, a sample assembly, a suction head assembly, an incubation and detection assembly and a mechanical arm group. The components of the application are integrated and installed on the mounting plate of the frame structure, the reaction cup assembly adopts gear and rack driving to realize automatic transfer of the cup rack, the reagent assembly realizes accurate temperature control of two kinds of reagents through a heating belt and a refrigeration module respectively, and the sample assembly and the suction head assembly adopt a pull-out structure, which is convenient for loading and unloading consumables. The application is compact in structure, convenient in operation and accurate in detection, and can be widely applied to thrombin detection scenes of medical institutions and scientific research laboratories at all levels.

The invention belongs to the technical field of medical diagnosis, and particularly relates to a visual column diagram construction method for identifying and diagnosing suppurative and tuberculous lumbar major muscle abscesses and interactive application. According to the invention, it is found that six indexes including serum uric acid (UA), thrombin time (TT), neutrophil absolute value (NEUT #), age (Age), fever (Fever) and erythrocyte sedimentation rate (ESR) are related to the type of the lumbar major muscle abscess, and a visual column diagram construction method for identifying and diagnosing suppurative and tuberculous lumbar major muscle abscess and interactive application are invented. And a first visual and quantifiable column diagram (Nomogram) prediction model for differential diagnosis of lumbar major muscle abscess is provided for the field. The technical problem that an abstract mathematical prediction model is converted into a visual graphic diagnosis tool which is intuitive in form and convenient to operate is solved, so that the visual graphic diagnosis tool can be separated from dependence on specific computing equipment, and clinical medical staff can quickly and accurately apply the visual graphic diagnosis tool on a diagnosis and treatment site conveniently.

The present invention relates to an absorbable internal hemostasis kit and an absorbable internal hemostatic agent, the kit comprising: a first syringe containing a powder mixture including gelatin, thrombin, and calcium chloride; a second syringe containing a mixed solution including a thermo-responsive gel, purified water, polyvinylpyrrolidone, polyethylene glycol, sodium chloride, and a protein extract obtained through a multi-stage decellularization process; and a connector for connecting the first syringe and the second syringe so that the powder mixture and the mixed solution can be combined, wherein the absorbable internal hemostatic agent according to the present invention promotes and activates primary physiological hemostasis through thrombin and provides a secondary physical hemostatic effect by gelling in a temperature range close to body temperature, while also offering additionally wound-healing effects, so as to achieve faster and more effective internal hemostasis.

The invention discloses an osteochondral integrated sponge scaffold as well as a preparation method and application thereof, and belongs to the field of osteochondral repair materials. The preparation method comprises the following steps: (1) mixing a collagen dispersion liquid and a polyvinyl alcohol solution, then adding a phosphate buffer solution and beta-tricalcium phosphate, and then obtaining lower-layer hydrogel through a pouring method; (2) mixing the collagen dispersion liquid with a polyvinyl alcohol solution, then adding a phosphate buffer solution, and then obtaining upper-layer hydrogel through a pouring method; and (3) pouring the upper-layer hydrogel on the lower-layer hydrogel, constructing a sponge scaffold through a multi-time freeze-thawing method and a freeze-drying method, and finally soaking the sponge scaffold in thrombin to obtain the osteochondral integrated sponge scaffold. The scaffold can adsorb platelet-rich plasma, has certain adhesion capacity, can realize in-situ fixation and biological integration at a defect part, can well recover physical and biological dysfunctions of bone joints and osteochondral bones, and provides a potential application idea for clinical treatment of osteochondral defects.

The invention relates to the technical field of enzyme engineering and bioactive peptide preparation, and discloses a preparation method of horse myocardial peptide with antioxidant and antithrombotic effects, and the preparation method comprises the following steps: S1, pretreating horse heart tissue to obtain horse myocardial pulp; s2, composite enzymatic hydrolysis and guiding agent regulation and control: adding a composite enzyme system and an anti-thrombus guiding agent into the horse myocardial plasma; and S3, affinity chromatography enrichment. According to the method disclosed by the invention, a composite enzymolysis, guide regulation and control and affinity enrichment synergistic process is constructed, so that the technical problems of incomplete tissue crushing, low enzymolysis efficiency, insufficient bioactive peptide yield and easiness in oxidation and inactivation caused by high proportion of IIa type muscle fibers and compact connective tissues of horse myocardium are solved in a targeted manner, and a compound enzyme system with a specific ratio is adopted to adapt to the characteristics of the horse myocardium; the enzymolysis efficiency is greatly improved, a cationic guiding agent is introduced to directionally improve the generation proportion of antithrombotic peptide fragments, efficient enrichment of target peptides is achieved by combining thrombin affinity chromatography, and the antithrombotic function is remarkably enhanced while high antioxidant activity is guaranteed.

The present invention provides a method for preventing, treating or alleviating bleeding caused by an anticoagulant using an anticoagulant reversal agent. The anticoagulant reversal agent has a structure as shown in formula I, can well inhibit bleeding complications caused by anticoagulants, for example, bleeding complications caused by anticoagulants such as a thrombin direct inhibitor, and has the characteristics of broad spectrum and high efficiency.

The application provides an emergency department wound hemostasis device, and relates to the technical field of wound hemostasis. The emergency department wound hemostasis device comprises an inflation hemostasis air bag, and the lower surface of the inflation hemostasis air bag is fixedly connected with a constant-temperature film bag. When working, the second liquid pump is started, the second liquid pump extracts water in the constant-temperature water storage tank through the second liquid pipeline, the water enters the inside of the constant-temperature film bag through the fourth liquid pipeline, the temperature of the wound is ensured, meanwhile, the first liquid pump is started, the first water valve on the first liquid pipeline is opened, the water in the constant-temperature film bag is extracted through the first liquid pipeline, and finally the water enters the inside of the constant-temperature water storage tank, so that the water in the constant-temperature film bag is always in a constant-temperature state, the temperature is close to the optimum point of the thrombin reaction, the platelet aggregation and fibrin formation can be accelerated, the method is safe and efficient, the tissue damage can be minimized, and the hemostasis speed is accelerated.

The invention relates to the technical field of biomedical materials, in particular to plasma matrix gel as well as a preparation method and application thereof. The preparation method comprises the following steps: centrifuging a whole blood sample in an environment containing negative oxygen ions to obtain the plasma matrix gel; or, the preparation method comprises the following steps: incubating the liquid plasma matrix in an environment containing negative oxygen ions to obtain the plasma matrix gel. According to the preparation method provided by the invention, the whole blood or liquid plasma matrix is treated in the negative oxygen ion environment, a platelet surface receptor mediated calcium signal channel can be efficiently activated, an endogenous blood coagulation cascade reaction is triggered to form the stable three-dimensional fibrin gel, the preparation method is simple and convenient to operate, and the preparation method is suitable for industrial production. The preparation method has the advantages that the addition of exogenous thrombin is completely avoided, the immunogenicity risk is avoided, the requirement on the material of the tube for preparing the plasma matrix is reduced, and a new solution is provided for the preparation of the plasma matrix gel.

This invention belongs to the field of surgical wound repair technology, and provides a multifunctional composite hydrogel, its preparation method, and its applications. The invention involves mixing fibrinogen, mussel byssal protein, calcium chloride, methacrylated dextran (Dextran-MA), thrombin, dithiothreitol, and a dispersing solvent. The resulting mixture is then gelled to obtain a multifunctional composite hydrogel. Thrombin specifically promotes the cross-linking of fibrinogen to form a biomolecular network; dithiothreitol efficiently mediates the formation of an organic macromolecular network through a Michael addition reaction of Dextran-MA; the introduction of Mfp5, utilizing its abundant catechol groups to form multiple coordination and covalent cross-links with organic / biomolecular macromolecules, significantly enhances the interfacial adhesion strength of the multifunctional composite hydrogel; simultaneously maintaining the biocompatibility, biodegradability, hemostatic properties, anti-inflammatory effects, and healing-promoting capabilities of the multifunctional composite hydrogel.

This invention provides a multifunctional nanoparticle formulation with thrombin targeting capability, comprising a gold nanoparticle matrix, a paramagnetic small molecule, and a thrombin-targeting nucleic acid aptamer. The thrombin-targeting nucleic acid aptamer endows the nanoparticle formulation with thrombin-targeting binding ability; the paramagnetic small molecule endows the nanoparticle formulation with T1-weighted magnetic resonance imaging capability and reactive oxygen species scavenging capability; the gold nanoparticle matrix endows the nanoparticle formulation with CT imaging capability and near-infrared photothermal conversion capability; the nanoparticle formulation can achieve thrombin-dependent enrichment within the thrombus.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of ginseng polypeptide. The ginseng active polypeptide is prepared by carrying out enzymolysis reaction on ginseng protein. The ginseng active polypeptide can effectively inhibit the activity of thrombin, so that the ginseng active polypeptide can be used as a thrombosis inhibitor to treat cardiovascular and cerebrovascular diseases such as myocardial infarction and ischemic stroke. The plant extract disclosed by the invention not only has an antithrombotic effect, but also has the advantages of no toxicity, no side effect, simplicity in preparation, easiness in absorption and the like, and the adverse effects of side effects of traditional chemical medicines such as bivalirudin and other thrombus inhibitors on human bodies are avoided.

This invention provides a composite biological dressing, its preparation method, and its application, belonging to the field of medical dressing technology. By employing a specific preparation method, this invention successfully combines components such as endometrial stem cells, human fibrinogen, human plasma cryoprecipitate, and thrombin solution with a silk scaffold material. The resulting composite biological dressing overcomes the problems of slow repair speed and poor efficacy of existing medical dressings. The composite biological dressing provided by this invention has a significant repair effect, avoiding unnecessary damage to the body. Repair is achieved simply by application, and it also enhances tissue regeneration and promotes the repair of existing tissue, providing comprehensive and rapid wound repair. The composite biological dressing obtained by this invention gradually dissolves after application, releasing endometrial stem cells that differentiate into new tissue, overcoming the drawback of significant cell loss after intravenous injection of existing stem cell injections.

The application relates to the field of biological medicine, and discloses a high-activity stable thrombin powder preparation and a preparation method thereof. The preparation is composed of spherical particles with a micron-nanometer hierarchical porous structure, and components include thrombin, a biocompatible skeleton material, a cryoprotective agent, a composite pore-forming agent and a surface amphiphilic modifier. The preparation method locks the activity through ultrasonic atomization and deep cold solidification of liquid nitrogen, and performs in-situ pore-forming through stepwise temperature control and sublimation drying. Through the synergistic effect of the hierarchical pore structure and the surface amphiphilic modification, the moisture resistance and flowability of the powder preparation are enhanced, the blood is rapidly perfused by utilizing capillary force, and the high-activity maintenance and second-level hemostasis response of the thrombin are realized.

The present invention relates to novel salts of Compound (II) and their crystals, as well as methods for preparing said salts and their crystals. The present invention further provides pharmaceutical compositions comprising the hydrochloride salt of Compound (II), and the use of said salts in the preparation of medicaments for the prevention and treatment of thrombin-mediated and / or thrombin-related diseases. TIFF2026504578000018.tif41170

The utility model discloses a kind of platelet-rich plasma spray gun devices, it relates to medical instrument technical field;To solve the secondary pollution, easy flocculation, mixing uneven, operator operation is not suitable for the problem existing in the mixing method of current platelet plasma and coagulation drug, the following technical scheme is proposed: a kind of platelet-rich plasma spray gun device, main syringe is detachably installed to handle, auxiliary syringe is parallel to main syringe setting, and the push end of both main syringe and auxiliary syringe and push plate are detachably fixed, the injection end of two and three-way joint two ports are connected, three-way joint other end is connected to spray head, and spray head spray outlet is provided with atomizing plug.The helical mixing column arranged in the spray head of the utility model makes platelet plasma and thrombin be immediately sprayed to wound after being fully mixed when using, which can prevent secondary pollution and maintain the therapeutic effect of platelets, and the design of push plate effectively reduces the pulling sensation of operator palm tiger mouth.

Methods for diagnosing atopic dermatitis (AD) by using thrombin and / or fibrinogen as biomarkers to determine the presence or severity of AD. Also provided are methods for treating AD by administering to a subject an effective amount of a thrombin inhibitor.

This invention relates to an IRAK4 degrading agent and its uses. This degrading agent can be used to treat or prevent diseases such as cancer, neurodegenerative diseases, viral diseases, autoimmune diseases, inflammatory diseases, hereditary diseases, hormone-related diseases, metabolic diseases, organ transplant-related diseases, immunodeficiency diseases, destructive bone diseases, proliferative diseases, infectious diseases, cell death-related conditions, thrombin-induced platelet aggregation, liver diseases, pathological immune conditions involving T cell activation, cardiovascular diseases, or CNS diseases.

This invention relates to the fields of enzyme engineering and bioactive peptide preparation technology, and discloses a method for preparing equine cardiac muscle peptides with antioxidant and antithrombotic effects, comprising the following steps: S1: Pre-treating equine heart tissue to obtain equine cardiac muscle plasma; S2: Compound enzymatic hydrolysis and directing agent regulation: Adding a compound enzyme system and an antithrombotic directing agent to the equine cardiac muscle plasma; S3: Affinity chromatography enrichment. This invention constructs a synergistic process of compound enzymatic hydrolysis, directing regulation, and affinity enrichment, specifically addressing the technical problems of incomplete tissue fragmentation, low enzymatic hydrolysis efficiency, insufficient yield of active peptides, and easy oxidative inactivation in equine cardiac muscle due to the high proportion of type IIa myofibrous fibers and dense connective tissue. Furthermore, it employs a specific ratio of compound enzyme system adapted to the characteristics of equine cardiac muscle, significantly improving enzymatic hydrolysis efficiency; introduces a cationic directing agent to directionally increase the proportion of antithrombotic peptides generated; and combines thrombin affinity chromatography to achieve efficient enrichment of target peptides, significantly enhancing antithrombotic function while ensuring high antioxidant activity.

Disclosed herein are methods of treatment for diseases and disorders with modulators of full-length or thrombin-cleaved osteopontin. Particularly disclosed herein are methods comprising treatment of tissue fibrosis (e.g., pulmonary, cardiac, liver, kidney, skin) with antibodies, or antigen-binding fragments thereof, that inhibit thrombin-cleavage of osteopontin or block the activity of thrombin cleavage fragments of osteopontin.

This invention relates to the fields of biomedical engineering and regenerative medicine, and particularly to a plasma matrix composite membrane activated by autologous keratinized epithelial tissue fragments, its preparation method, and its applications. The composite membrane is obtained by mixing autologous keratinized epithelial tissue fragments with a liquid autologous plasma matrix in a specific ratio, utilizing tissue factors on the surface of the tissue fragments to trigger platelet activation, spontaneously forming a gel, and then pressing it into a membrane. This invention is the first to utilize the biointerface of autologous keratinized epithelial tissue fragments to activate platelets, eliminating the need for exogenous thrombin and achieving completely autologous in-situ gelation. The composite membrane formed after pressing possesses excellent mechanical properties and barrier function, and can be prepared in a single operation in the operating room without the need for in vitro cell culture. The composite membrane of this invention is used for oral soft tissue augmentation, such as widening of keratinized gingiva and alveolar ridge soft tissue augmentation, and has advantages such as no donor site damage, high biosafety, and ease of operation.