24 results about "Diabetic ulcer" patented technology

The invention discloses multifunctional hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method of the multifunctional hydrogel comprises the following steps: preparing an HAMA solution, a PVA solution and a MnO2 solution, constructing C18 / SDS / NaCl micelles, concentrating, mixing the components, adding a photoinitiator, carrying out ultraviolet curing, and carrying out circulating freeze thawing and cationic crosslinking to obtain a final product. The hydrogel disclosed by the invention has physiological-adaptive mechanical properties, strong adhesion in air / water, 99.8% of staphylococcus aureus resistance rate and 23.6% of ROS clearance rate in 12 hours, nearly 100% healing of mouse diabetic ulcer can be realized in 16 days, and the hydrogel is suitable for repairing chronic difficult-to-heal wounds and has remarkable clinical application value.

The invention belongs to the technical field of biomedical materials. The invention provides a composite nano preparation for treating diabetic ulcer and promoting wound healing and a preparation method thereof. The preparation method comprises the following steps: mixing tetra (4-carboxyl phenyl) porphyrin and FeCl3. 6H2O for reaction, then adding a mixed solution composed of benzoic acid and 3, 5-dicarboxyphenylboronic acid for reaction, and then performing centrifugal drying; dispersing in water, adding a chloroplatinic acid solution, stirring, adding a sodium borohydride solution, continuously stirring, and centrifugally drying; stirring with a mixture of curcumin, flavonoid glycoside and astragaloside in ethanol, and carrying out centrifugal drying; and finally, stirring with glucose oxidase in water, and centrifugally drying. The composite nano preparation provided by the invention has an excellent antibacterial effect on multi-drug-resistant bacteria, can effectively destroy a biological membrane, can well treat diabetic ulcer and promote wound healing, and has a relatively good application prospect.

The invention provides a traditional Chinese medicine composition as well as a preparation method and application thereof, and particularly belongs to the technical field of medicine preparation. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises mulberry leaf water and a second component, the second component comprises mulberry leaf powder and / or cortex phellodendri powder; all the components are independently subpackaged; the preparation method of the mulberry leaf powder comprises the following steps: performing charcoal burning and crushing on mulberry leaves to obtain mulberry leaf powder; the preparation method of the cortex phellodendri powder comprises the following steps: calcining and crushing cortex phellodendri to obtain cortex phellodendri powder; the preparation method of the mulberry leaf water comprises the following steps: mixing mulberry leaves and water, decocting, and filtering to obtain the mulberry leaf water. The traditional Chinese medicine composition disclosed by the invention can play a local hypoglycemic role, promote rapid healing of an ulcer surface, realize treatment of diabetic ulcer, and relieve pain and economic burden of a patient suffering from diabetic skin ulcer.

The application discloses a kind of fusion polypeptide SynExo for targeted delivery and its engineering exosome preparation method.The fusion polypeptide includes PDGFR beta transmembrane domain (FIASFFLAVVTLLVLLYF), photocontrol cross-linking site 4-azidophenylalanine (AzF) and double-targeting peptide CRGDKGPDC, is connected in series by flexible linker GGGGS, realizes the efficient anchoring, accurate targeting and space-time controllable delivery of exosome.The high hydrophobicity (GRAVY value +2.3) of PDGFR beta transmembrane domain makes its anchoring efficiency increase by 2.7 times, AzF generates azinome intermediate under 365nm light to realize irreversible cross-linking, double-targeting peptide targets integrin αvβ3 and ECM1 by cRGD (Kd=25nM) and KGPDC (Kd=1.2nM) synergistically, and penetration depth reaches 180 μm.Engineering exosome realizes AzF accurate incorporation by lentivirus packaging combined with orthogonal tRNA system, and in diabetic ulcer model, targeted enrichment efficiency reaches 6.8 times of unmodified group, and wound closure rate is 92.5%, which is significantly better than that of control group.The application provides an innovative platform for targeted treatment of diseases such as tumors and diabetic ulcers.

The invention belongs to the technical field of biological medicines, and relates to application of centella asiatica exosomes in preparation of medicines for preventing, relieving or treating diabetic ulcers. The centella asiatica exosome can reduce inflammatory response, increase secretion of collagen, promote migration of epithelial cells, inhibit scar formation, inhibit growth of fibroblasts, promote matrix deposition, promote epithelial regeneration of wounds and effectively promote healing of diabetic ulcers.

The invention relates to the technical field of biology, in particular to a compound polysaccharide-fish skin source collagen scaffold as well as a preparation method and application thereof. According to the preparation method, tilapia skin is taken as a raw material, collagen is extracted and prepared under the condition that the temperature is lower than the denaturation temperature of the collagen, then astragalus polysaccharide and angelica polysaccharide are selected as drugs carried by the collagen scaffold, and the fish-derived collagen scaffold with biological activity is constructed and used for loading bone marrow mesenchymal stem cells (BMSCs). The oxidative stress injury of the BMSCs can be effectively reduced, the proliferation of the BMSCs can be promoted, the diabetic ulcer wound surface can be repaired, and the wound surface healing can be promoted. The stem cell scaffold with biological activity is constructed by utilizing the traditional Chinese medicines astragalus polysaccharide and angelica polysaccharide for the first time, and the advantages of the traditional Chinese medicines are exerted; meanwhile, the collagen adopted by the invention is derived from fish skin instead of mammals, so that religious barriers, mammalian infectious diseases and the like are avoided.

This invention discloses an engineered probiotic for promoting wound healing, its construction method, and its application. The engineered probiotic is Escherichia coli Nissle 1917 (… Escherichia coli This invention provides an engineered probiotic for promoting wound healing. The engineered bacteria, derived from the Nissle 1917 (EcN) strain with its ThyA gene knocked out, has a nucleotide sequence shown in SEQ ID NO.1. The engineered bacteria constructed in this invention retain the beneficial immunomodulatory activity of EcN (particularly its potential ability to promote the transformation of macrophages into a pro-repair phenotype), while allowing for precise control of its colonization in vivo. This allows for the safe and controllable correction of the abnormal inflammatory microenvironment of diabetic ulcers, thereby effectively promoting wound healing.

The application discloses a novel brown algae polysaccharide SARP for promoting healing of diabetic ulcers and a preparation and application thereof. A novel SARP is obtained through Sephacryl S-300 / HR gel column separation and purification, and the molecular weight of the SARP is about 45.4 kDa, which is composed of mannuronic acid (76.56%), guluronic acid (18.89%) and a small amount of glucuronic acid (4.55%). Compared with alginate separated from other brown algae, the SARP has a higher content of mannuronic acid. The SARP shows a better in-vitro antioxidant capacity, and can obviously reverse the cell damage of HUVECs caused by H2O2 and high sugar in the HUVECs. In addition, the SARP can promote the migration and tubular formation of the HUVECs. In-vivo experiments further show that the SARP can promote the healing of diabetic ulcer in rats, and can reduce the oxidative stress, inflammation level and lipid disorder in the rats. The SARP has a simple purification route, can be prepared in a large amount, and is a very promising novel drug for treating diabetic foot ulcers.

The invention discloses a pharmaceutical composition (a gel) for diabetic ulcer. The gel comprises Fusidic acid and a biopolymer in the form of Chitosan, and a peptide in the form of hydrolyzed Collagen. Specifically, the gel comprises an antibacterial, Fusidic acid with a biopolymer, Chitosan and natural peptide of Type I Collagen. The composition is found to be therapeutically highly effective as a result of the unique and desirable physical, chemical and therapeutic properties of collagen embedded in chitosan gel framework with Fusidic acid.The composition is stable despite incorporation of Chitosan & hydrolyzed Collagen into a process of Fusidic acid of very low particle size. The composition has 0.1% (w / w) to 5% (w / w) of Sodium Fusidate and Chitosan of molecular weight in the range of 250,000 Da to 500,000 Da in the weight proportion of 1.25% (w / w), and the hydrolyzed Collagen of molecular weight in the range of 3000 Da to 6000 Da in the weight proportion of 0.2% (w / w).

This invention provides a natural wound ointment dressing to address the problem of applying natural ingredients to the treatment of pressure sores, diabetic ulcers, and burn wounds; the natural wound ointment dressing is composed of the following natural ingredients: curcumin, Manuka honey, tea tree oil, and aloe vera gel.

The application belongs to the field of biomedical polymer materials and regenerative medicine, and particularly relates to an intelligent hydrogel for treating skin tissue damage, a preparation method and a medicine thereof, and application thereof. The intelligent hydrogel comprises a hydrogel support and a functional module. The hydrogel support takes methacrylated gelatin as a matrix and forms a stable three-dimensional network structure through photo-crosslinking. The functional module comprises two core DNA chains, one of which is a ZnR-Cage chain with a sulfhydryl group directly or indirectly connected to one end of the chain, and the other is a CD44-link chain. The two chains form a non-active assembly through base complementary pairing. The intelligent hydrogel has many advantages such as strong spatiotemporal controllability, overcoming drug burst defects, efficient cascade repair, realizing targeted synergistic treatment, efficient integration, excellent stability and clinical transformation potential. It is particularly suitable for clinical treatment of complex wounds such as diabetic ulcers and infected wounds.

This invention discloses a visual, multi-dimensional monitoring hydrocolloid dressing for wounds, along with its preparation and application methods. Belonging to the field of medical dressing technology, the dressing comprises, from the wound contact side to the outer side, a wound adhesion layer, an isolation and protective layer, an indicator strip layer, and a surface protective film, all four layers integrally cross-linked. The wound adhesion layer features a zoned gradient absorbent structure, a hydrophilic silicone oil film, and a modular, customizable main core + auxiliary core structure, and is loaded with antibacterial components. The isolation and protective layer eliminates interference from the hydrocolloids in detection and achieves antibacterial and sustained-release effects. The indicator strip layer integrates multiple independent functional areas for qualitative and quantitative exudate analysis and microenvironment monitoring. This invention also discloses a method for preparing the dressing in layers and for integral cross-linking. This invention integrates nursing care, monitoring, and antibacterial and anti-inflammatory functions, providing accurate monitoring, strong adaptability, and avoiding secondary wound damage. It is suitable for the nursing monitoring of chronic wounds such as diabetic ulcers, pressure ulcers, and burns, and has extremely high clinical application value.

The invention discloses a space-time regulation hydrogel dressing for rapid scar-free healing of diabetic ulcer and a preparation method of the space-time regulation hydrogel dressing. The preparation method of hydrogel comprises the following steps: synthesizing polydopamine coated hollow manganese dioxide (pMnO2) nanoparticles; synthesizing a temperature-sensitive copolymer with an adjustable low critical phase transition temperature (LCST); a solvent-anti-solvent precipitation method is adopted to prepare a vinipofen nano-microsphere (Vet NPs); through dynamic chemical bonds, the hydrogel dressing with precise space-time regulation and control is prepared. The gel can realize multi-stage programmable release of pMnO2 and Vet NPs, can resist bacteria and oxidation in an early stage, can improve an inflammatory microenvironment, can inhibit fibrosis in repair and remodeling stages, can dynamically regulate and control fibroblast behaviors, and can promote scar-free regeneration of diabetic ulcers.

The present application relates to the use of gypenoside A in the preparation of a medicament for treating diabetic ulcers. The compounds of the present application are useful for treating diabetic ulcers.

The present disclosure is concerned with compositions comprising insulin and a pharmaceutically acceptable topical carrier. The present disclosure is also concerned with methods of using these compositions for the treatment of a skin ailment such as a diabetic ulcer. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The invention provides a preparation method of pH-responsive injectable algal polysaccharide hydrogel for drug delivery based on dynamic covalent bonds and application of the pH-responsive injectable algal polysaccharide hydrogel in chronic wounds, especially diabetic ulcer wounds. The preparation method of the dressing is simple and easy to implement, the prepared hydrogel has good water absorption and swelling rate, the hydrogel has pH responsiveness, intelligent drug release of the hydrogel according to the pH change of the wound microenvironment is facilitated, tissue regeneration is effectively promoted, and infection is inhibited. The hydrogel dressing not only has the characteristics of good air permeability, good hygroscopicity and the like, but also has the capability of inhibiting the growth of bacteria, and the hydrogel has injectability and can fill irregular wound areas and promote in-situ tissue regeneration so as to finally promote the healing of chronic wounds. The traditional Chinese medicine composition is especially suitable for clinically widely existing chronic difficult-to-heal wounds, especially for treatment and nursing of diabetic ulcers.

The invention discloses a composite gel for promoting wound healing. The composite gel comprises at least one growth factor and at least one therapeutic gas. The composite gel can maximize the synergistic effect of the gas and the growth factors by selectively adjusting the combination of the gas and the growth factors, so as to meet the treatment requirements of different types of wounds and different healing stages. The therapeutic gases can be selected from oxygen, hydrogen, nitric oxide, hydrogen sulfide and the like, are distributed in the gel in the form of microbubbles and are slowly released to improve the local microenvironment of the wound. In addition, the growth factors can comprise epidermal growth factors (EGF), fibroblast growth factors (FGF), vascular endothelial growth factors (VEGF) and the like, and cell proliferation, migration and new angiogenesis are promoted. The preparation method of the composite gel comprises the following steps: selecting a proper matrix material, dissolving the matrix material with growth factors, and then injecting therapeutic gas to form gel containing bubbles. The composite gel can effectively improve the wound healing speed, and is especially suitable for chronic or refractory wounds such as diabetic ulcers, burns, surgical incisions and the like.

This invention belongs to the field of pharmaceutical technology, specifically disclosing an aerogel material loaded with turmeric-derived extracellular vesicle nanoparticles and its preparation and application. The material comprises an aerogel and turmeric-derived extracellular vesicle nanoparticles loaded on the aerogel. The loading amount of extracellular vesicle nanoparticles in each 10 mg of aerogel ranges from 10 to 6000 micrograms. The aerogel material possesses advantages such as a wide range of adjustable loading amounts of turmeric-derived extracellular vesicle nanoparticles, high release efficiency, good permeability, good biocompatibility, lightweight, and room-temperature storage. It is a biomaterial with practical application potential and can be made into medical wound dressings for application in wound repair, especially in diabetic ulcers, effectively solving the problem of poor wound healing caused by the inflammatory and oxidative stress microenvironment of diabetic wounds.

The invention discloses a fusion polypeptide SynExo for targeted delivery and a preparation method of an engineered exosome of the fusion polypeptide SynExo. The fusion polypeptide comprises a PDGFR [beta] transmembrane domain (FIASFFLVTLLVLLYF), a light-operated crosslinking site 4-azido phenylalanine (AzF) and a dual-targeting peptide CRGDKGPDC, which are connected in series through a flexible linker GGGGS, so that efficient anchoring, precise targeting and space-time controllable delivery of an exosome are realized. The anchoring efficiency of the PDGFR beta transmembrane domain is improved by 2.7 times due to high hydrophobicity (GRAVY value + 2.3), AzF generates a nitrogen-based intermediate under 365nm illumination to realize irreversible crosslinking, the dual-targeting peptide synergistically targets integrin alpha v beta 3 and ECM1 through cRGD (Kd = 25nM) and KGPDC (Kd = 1.2 nM), and the penetration depth reaches 180 microns. The engineered exosome realizes accurate doping of AzF through lentivirus packaging in combination with an orthogonal tRNA system, the targeted enrichment efficiency in a diabetic ulcer model is 6.8 times that of an unmodified group, and the wound closure rate is 92.5%, which is significantly superior to that of a control group. The invention provides an innovative platform for targeted therapy of diseases such as tumors and diabetic ulcers.

This invention belongs to the field of catalytic materials technology, specifically relating to a porous carbon biocatalytic material loaded with rhodium-iron bimetallic active sites, its preparation method, and its applications. The porous carbon biocatalytic material comprises a spiky porous carbon substrate, and rhodium clusters and iron single atoms uniformly loaded on its surface, forming Rh-Fe bimetallic active sites with charge transfer effects. The porous carbon biocatalytic material of this invention exhibits peroxidase-like and NADH oxidase-like activities under acidic conditions, and glucose oxidase-like, catalase-like, and superoxide dismutase-like activities under neutral conditions. It can efficiently kill bacteria and adsorb bacterial antigens, preventing macrophages from excessively polarizing to a pro-inflammatory state, ultimately promoting tissue regeneration and achieving ultra-rapid healing of inflammatory diabetic ulcers, with good biocompatibility.

A medicinal composition in the form of an aqueous solution contains 1-deoxy-1-N-methylammonium-D-glucitol succinate, inosine, nicotinamide and methionine in the following proportions: 7.5-15.0 g / l 1-deoxy-1-N-methylammonium-D-glucitol succinate; 1.50-3.0 g / l inosine; 0.53-1.05 g / l methionine; 0.19-0.38 g / l nicotinamide; water for injection to 1 litre. Also disclosed is a method of producing a parenteral preparation containing the claimed medicinal composition. The composition exhibits anti-diabetic, nephroprotective and wound-healing activity and can be used in the long-term treatment of diabetes mellitus and attendant complications, particularly diabetic nephropathy and chronic skin wounds caused by soft tissue damage (diabetic ulcers).

1. The name of the design product: diabetic ulcer wound monitoring device. 2. The use of the design product: for dynamically monitoring the wound data of diabetic ulcer wounds. 3. The design points of the design product: in the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view.

The application discloses a marine organism peptide capable of effectively promoting skin wound repair, and a preparation method and application thereof. The marine organism peptide has good skin compatibility, can significantly promote cell proliferation, migration and vascular formation, effectively reduces inflammatory reaction, promotes high-quality repair of wounds, and reduces scar formation. The preparation method of the marine organism peptide comprises the following steps: taking a fresh and live soft body of a marine shellfish, performing protease enzymolysis, ultrafiltration and dextran gel separation, discarding an elution single peak in the last step, collecting or mixing other elution components, and freeze-drying to obtain the marine organism peptide. The method is simple, low in cost, green and environment-friendly, short in production period, and suitable for industrial production. The marine organism peptide can be applied to medicines, medical biomaterials and cosmetics, has a wide application range, and has a remarkable effect on promoting regeneration and repair of burn, scald, cutting, traffic, surgical, war injury, firearm injury combined with seawater immersion injury, diabetic ulcer, inflammatory ulcer, pressure ulcer and chronic refractory wounds.

The invention belongs to the technical field of biomedical application, and particularly relates to a 3D dynamic culture method of extracellular vesicles and application of the 3D dynamic culture method. High-yield extracellular vesicles capable of remarkably promoting vascular neural network repair are obtained by adopting root tip tooth papilla stem cells and a zinc-doped bioactive glass extraction environment in combination with a 3D dynamic culture mode, SCAPs serve as EVs parent cells, the individual specificity of the obtained EVs can be guaranteed, vascular neural differentiation and repair of target tissue can be effectively promoted, and the vascular neural network repair effect is improved. The yield of the EVs protein is obviously improved; and the vascular / nerve repairing function is directionally enhanced. Besides, SD rat type II diabetes pressure sores and ulcers are adopted to simulate DU formation of clinical patients, the vascular neural network regeneration promoting performance, ECM and collagen maturation promoting performance and DU healing accelerating performance of the ZnBG-EVs-loaded dressing are evaluated, and the application prospect in the EVs preparation and DU treatment directions is huge.